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Quantitative predictions of natural and induced phenomena in fractured rock is one of the great challenges in the Earth and Energy Sciences with far-reaching economic and environmental impacts. Fractures occupy a very small volume of a subsurface formation but often dominate fluid flow, solute transport and mechanical deformation behavior. They play a central role in CO2 sequestration, nuclear waste disposal, hydrogen storage, geothermal energy production, nuclear nonproliferation, and hydrocarbon extraction. These applications require predictions of fracture-dependent quantities of interest such as CO2 leakage rate, hydrocarbon production, radionuclide plume migration, and seismicity; to be useful, these predictions must account for uncertainty inherent in subsurface systems. Here, we review recent advances in fractured rock research covering field- and laboratory-scale experimentation, numerical simulations, and uncertainty quantification. We discuss how these have greatly improved the fundamental understanding of fractures and one's ability to predict flow and transport in fractured systems. Dedicated field sites provide quantitative measurements of fracture flow that can be used to identify dominant coupled processes and to validate models. Laboratory-scale experiments fill critical knowledge gaps by providing direct observations and measurements of fracture geometry and flow under controlled conditions that cannot be obtained in the field. Physics-based simulation of flow and transport provide a bridge in understanding between controlled simple laboratory experiments and the massively complex field-scale fracture systems. Finally, we review the use of machine learning-based emulators to rapidly investigate different fracture property scenarios and accelerate physics-based models by orders of magnitude to enable uncertainty quantification and near real-time analysis.

On behalf of the journal, AGU, and the scientific community, the editors would like to sincerely thank those who reviewed the manuscripts for Geophysical Research Letters in 2019. The hours reading and commenting on manuscripts not only improve the manuscripts but also increase the scientific rigor of future research in the field. We particularly appreciate the timely reviews in light of the demands imposed by the rapid review process at Geophysical Research Letters. With the revival of the “major revisions” decisions, we appreciate the reviewers' efforts on multiple versions of some manuscripts. With the advent of AGU's data policy, many reviewers have helped immensely to evaluate the accessibility and availability of data associated with the papers they have reviewed, and many have provided insightful comments that helped to improve the data presentation and quality. We greatly appreciate the assistance of the reviewers in advancing open science, which is a key objective of AGU's data policy. Many of those listed below went beyond and reviewed three or more manuscripts for our journal, and those are indicated in italics.

On behalf of the journal, AGU, and the scientific community, the Editors would like to sincerely thank those who reviewed manuscripts for Geophysical Research Letters in 2018. The hours reading and commenting on manuscripts not only improves the manuscripts but also increases the scientific rigor of future research in the field. We particularly appreciate the timely reviews, in light of the demands imposed by the rapid review process at Geophysical Research Letters. With the revival of the “major revisions” decisions, we appreciate the reviewers' efforts on multiple versions of some manuscripts. Many of those listed below went beyond and reviewed three or more manuscripts for our journal, and those are indicated in italics. In total, 4,484 referees contributed to 7,557 individual reviews in journal. Thank you again. We look forward to the coming year of exciting advances in the field and communicating those advances to our community and to the broader public.

Two outstanding questions for the future of the Greenland Ice Sheet are (a) how enhanced meltwater draining beneath the ice will impact the behavior of large tidewater glaciers, and (b) to what extent tidewater glacier velocity is driven by changes at the terminus versus changes in sliding velocity due to meltwater. We present a two‐way coupled framework to simulate the nonlinear feedbacks of evolving subglacial hydrology and ice dynamics using the Subglacial Hydrology And Kinetic, Transient Interactions (SHAKTI) model within the Ice‐sheet and Sea‐level System Model (ISSM). Through coupled simulations of Helheim Glacier, we find that terminus effects dominate the seasonal velocity pattern up to 15 km from the terminus, while hydrology drives the velocity response upstream. With increased melt, the hydrology influence yields seasonal acceleration of several hundred meters per year in the interior, suggesting that hydrology will play an important role in future mass balance of tidewater glaciers. Plain Language Summary: Water draining under glaciers and ice sheets affects the friction between the ice and the bed, and controls how fast the ice can slide into the ocean, contributing to sea‐level rise. We present a framework for simulating the feedbacks between hydrology and ice flow. We investigate the relative influence of changes at the terminus of the glacier where it meets the ocean, versus changes in meltwater drainage, in determining how fast the glacier moves. Our modeling of Helheim Glacier in southeast Greenland highlights the importance of terminus effects up to 15 km from the terminus, and hydrology farther upstream, with increased melt yielding higher inland acceleration. These results suggest that meltwater will play an increasingly important role in the future behavior of glaciers. Key Points: We couple a subglacial hydrology model with an ice flow model to simulate the relationship between sliding velocity and effective pressureTerminus effects at Helheim Glacier drive velocity up to 15 km upstream, but seasonal hydrology controls velocity patterns further inlandIncreased melt accelerates ice inland of the main trunk, implying importance of hydrology in tidewater glacier future mass balance [ABSTRACT FROM AUTHOR]

Ice shelf fractures frequently terminate where they encounter suture zones, regions of material heterogeneity that form between meteoric inflows in ice shelves. This heterogeneity can consist of marine ice, meteoric ice with modified rheological properties, or the presence of fractures. Here, we use radar observations on the Larsen C Ice Shelf, Antarctica, to investigate (i) the termination of a 25 km long rift in the Churchill Peninsula suture zone, which was found to contain ~60 m of accreted marine ice, and (ii) the along-flow evolution of a suture zone originating at Cole Peninsula. We determine a steady state field of basal melting/freezing rates and apply it to a flowline model to delineate the along-flow evolution of layers within the ice shelf. The thickening surface wedge of locally accumulated meteoric ice, which likely has limited lateral variation in its mechanical properties, accounts for ~60% of the total ice thickness near the calving front. Thus, we infer that the lower ~40% of the ice column and the material heterogeneities present there are responsible for resisting fracture propagation and thereby delaying tabular calving events, as demonstrated in the >40 year time series leading up to the 2004/2005 calving event for Larsen C. This likely represents a highly sensitive aspect of ice shelf stability, as changes in the oceanic forcing may lead to the loss of this heterogeneity. ©2014. American Geophysical Union. All Rights Reserved.

A key mechanism for the rapid collapse of both the Larsen A and B Ice Shelves was meltwater-driven crevasse propagation. Basal crevasses, large-scale structural features within ice shelves, may have contributed to this mechanism in three important ways: i) the shelf surface deforms due to modified buoyancy and gravitational forces above the basal crevasse, creating >10 m deep compressional surface depressions where meltwater can collect, ii) bending stresses from the modified shape drive surface crevassing, with crevasses reaching 40 m in width, on the flanks of the basal-crevasse-induced trough and iii) the ice thickness is substantially reduced, thereby minimizing the propagation distance before a full-thickness rift is created. We examine a basal crevasse (4.5 km in length, ∼230 m in height), and the corresponding surface features, in the Cabinet Inlet sector of the Larsen C Ice Shelf using a combination of high-resolution (0.5 m) satellite imagery, kinematic GPS and in situ ground penetrating radar. We discuss how basal crevasses may have contributed to the breakup of the Larsen B Ice Shelf by directly controlling the location of meltwater ponding and highlight the presence of similar features on the Amery and Getz Ice Shelves with high-resolution imagery. © 2012. American Geophysical Union. All Rights Reserved.

Nucleoside diphosphate kinase (Ndk/NDK/NDPK) is known to possess pleiotropic functions, one of which is that as a protein kinase, and has been shown to be involved in stress tolerance in plants. To assess its role in the cyanobacterium Nostoc PCC 7120, which is hitherto unreported, recombinant strain overexpressing Ndk, Anndk + was generated. Phosphoproteomic analysis of Anndk + and its comparison with that of the vector control, AnpAM, revealed differential phosphorylation at S/T/Y sites of proteins belonging to varied functional groups, with over 17 % phosphoproteins involved in photosynthesis. A total of 177 phosphopeptides and 117 phosphoproteins were identified, including newly identified phosphopeptides in any cyanobacteria. Compared to AnpAM, the Anndk + cells exhibited (i) lower photosynthetic efficiency and electron transport rate at low PAR (photosynthetically active radiation), (ii) no change in photochemical quenching across PAR, (iii) but distinct non-photochemical quenching [zero Y(NPQ) and high Y(NO) in Anndk + and high Y(NPQ) and low (NO) in AnpAM], and (iv) increased tolerance to γ-radiation, oxidative, salt and DCMU stresses. The observed modulation of phosphoproteome linked to physiological changes upon overexpression of Ndk in Nostoc could be a combination of direct protein kinase activity of Ndk and/or indirectly through other protein kinases and phosphatases whose phosphorylation status is mediated by Ndk. This is the first report on a direct correlation between Ndk levels, phosphorylation status of proteins and stress tolerance in any cyanobacteria., Competing Interests: Declaration of competing interest Authors report no conflict of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Background: Studies have shown a relationship between oestrogen and Alzheimer's disease. However, there is neither clear nor strong evidence on the use of oestrogen-only therapy in reducing the risk of Alzheimer's disease., Aims: To assess the effects of oestrogen-only therapy on reducing the risk of Alzheimer's disease., Method: Inclusion criteria was determined with the PICO framework. Outcome was cognitive function measured by neuropsychological tests and strict protocols. Exclusion criteria included non-Alzheimer's dementia, progesterone-only therapy and pre-menopausal women. Searches were conducted in nine electronic healthcare databases, last searched in July 2022. Quality assessments conducted on randomised controlled trials (RCTs) were performed with the GRADE assessment, and cohort studies and case-control studies were assessed with the Newcastle-Ottawa Scale. Extracted data were used to analyse participants, interventions and outcomes., Results: Twenty-four studies satisfied the search criteria (four RCTs, nine cohort studies, 11 case-control studies). Fifteen studies showed positive associations for oestrogen-only therapy reducing the risk of Alzheimer's disease, and the remaining nine found no evidence of association., Conclusions: Fifteen studies showed that oestrogen-only therapy effectively reduced the risk of Alzheimer's disease, whereas nine showed no correlation. Studies also investigated oestrogen-related variables such as length of oestrogen exposure, being an apolipoprotein E ε4 carrier and concomitant use of non-steroidal anti-inflammatory drugs, and their role in neuroprotection. This review was limited by the limited ranges of duration of oestrogen treatment and type of oestrogen-only therapy used. In conclusion, oestrogen-only therapy has potential for use in preventing Alzheimer's disease, although current evidence is inconclusive and requires further study.

Exopolysaccharides (EPS) produced by bacterial species are an important component of bacteria's survival strategy. Synthesis of EPS, principal component of extracellular polymeric substance, occurs through multiple pathways involving multitude of genes. While stress-induced concomitant increase in exoD transcript levels and EPS content have been shown earlier, experimental evidence for direct correlation is lacking. In the present study, role of ExoD in Nostoc sp. strain PCC 7120 was evaluated by generating a recombinant Nostoc strain AnexoD + , wherein the ExoD (Alr2882) protein was constitutively overexpressed. AnexoD + exhibited higher EPS production, propensity for formation of biofilms and tolerance to Cd stress compared to vector control AnpAM cells. Both Alr2882 and its paralog All1787 exhibited 5 transmembrane domains, with only All1787 predicted to interact with several proteins in polysaccharide synthesis. Phylogenetic analysis of orthologs of these proteins across cyanobacteria indicated that the two paralogs Alr2882 and All1787 and their corresponding orthologs arose divergently during evolution, and could have distinct roles to perform in the biosynthesis of EPS. This study has thrown open the possibility of engineering overproduction of EPS and inducing biofilm formation through genetic manipulation of EPS biosynthesis genes in cyanobacteria, thus building a cost-effective green platform for large scale production of EPS., Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest., (Copyright © 2023 Elsevier B.V. All rights reserved.)

LexA, a well-characterized transcriptional repressor of SOS genes in heterotrophic bacteria, has been shown to regulate diverse genes in cyanobacteria. An earlier study showed that LexA overexpression in a cyanobacterium, Anabaena sp. PCC7120 reduces its tolerance to Cd stress. This was later shown to be due to modulation of photosynthetic redox poising by LexA under Cd stress. However, due to the global regulatory nature of LexA and the prior prediction of AnLexA-box in a few heavy metal-responsive genes, we speculated that LexA has a broad role in Cd tolerance, with regulation over a variety of Cd stress-responsive genes in addition to photosynthetic genes. Thus, to further expand the knowledge on the regulatory role of LexA in Cd stress tolerance, a cytosolic proteome profiling of Anabaena constitutively overexpressing LexA upon Cd stress was performed. The proteomic study revealed 25 differentially accumulated proteins (DAPs) in response to the combined effect of LexA overexpression and Cd stress, and the other 11 DAPs exclusively in response to either LexA overexpression or Cd stress. The 36 identified proteins were related with a variety of functions, including photosynthesis, C-metabolism, antioxidants, protein turnover, post-transcriptional modifications, and a few unknown and hypothetical proteins. The regulation of LexA on corresponding genes, and six previously reported Cd efflux transporters, was further validated by the presence of AnLexA-boxes, transcript, and/or promoter analyses. In a nutshell, this study identifies the regulation of Anabaena LexA on several Cd stress-responsive genes of various functions, hence expanding the regulatory role of LexA under Cd stress., Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Regulation of DNA repair genes in cyanobacteria is an unexplored field despite some of them exhibiting high radio-resistance. With RecF pathway speculated to be the major double strand break repair pathway in Nostoc sp. strain PCC7120, regulation of recF, recO and recR genes was investigated. Bioinformatic approach-based identification of promoter and regulatory elements was validated using qRT-PCR analysis, reporter gene and DNA binding assays. Different deletion constructs of the upstream regulatory regions of these genes were analysed in host Nostoc as well as heterologous system Escherichia coli. Studies revealed: (1) Positive regulation of all three genes by NtcA, (2) Negative regulation by LexA, (3) Involvement of contiguous heptamer repeats with/without its yet to be identified interacting partner in regulating (i) binding of NtcA and LexA to recO promoter and its translation, (ii) transcription or translation of recF, (4) Translational regulation of recF and recO through non-canonical and distant S.D. sequence and of recR through a rare initiation codon. Presence of NtcA either precludes binding of LexA to AnLexA-Box or negates its repressive action resulting in higher expression of these genes under nitrogen-fixing conditions in Nostoc. Thus, in Nostoc, expression of recF, recO and recR genes is intricately regulated through multiple regulatory elements/proteins. Contiguous heptamer repeats present across the Nostoc genome in the vicinity of start codon or promoter is likely to have a global regulatory role. This is the first report detailing regulation of DSB repair genes in any algae., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Owing to their crucial role in genome maintenance, RecQ helicases are ubiquitous and present across organisms. Though the multiplicity of RecQ helicases is well known in higher organisms, it is rare among bacteria. The ancient cyanobacterium Nostoc sp. strain PCC7120 was found to have three annotated RecQ helicases. This study aims at understanding its structural differences and evolution through bioinformatics approach and functionality through expression analysis studies. Nostoc RecQ helicases were found to be transcriptionally regulated by LexA and DNA damage inducing stresses. Bioinformatic analysis revealed that all three RecQ helicases of Nostoc possess helicases_C and Zn +2 -binding domains. Two of the helicases (AnRecQ and AnRecQ2) lacked the complete RQC and HRDC domains, and AnRecQ2 had an additional Phosphoribosyl transferase domain (Pribosyltran), also seen in RecQ-like helicase (RqlH) protein of Mycobacterium smegmatis. AnRecQ1, which was similar to most bacterial RecQ helicases, differed in having a long C-terminal tail. STRING analysis revealed that the proteins also differed in their predicted protein interactome. Phylogenetic analysis suggested that the multiple recQ genes may have been acquired through duplication and acquisition of additional domains from the smallest of the RecQ helicases (AnRecQ) to cater multiple functions required to deal with the harsh environmental conditions. In course of evolution, however, the multiplicity was lost with the modern-day bacteria and lower eukaryotes which retained fewer RecQ helicases, while further duplication of the acquired RECQ occurred in higher animals and plants to deal with cellular complexity., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

High radioresistance of the cyanobacterium, Anabaena sp. PCC7120 has been attributed to efficient DNA repair, protein recycling, and oxidative stress management. However, the regulatory network involved in these batteries of responses remains unexplored. In the present study, the role of a global regulator, LexA in modulating gamma (γ)-radiation stress response of Anabaena was investigated. Comparison of the cytosolic proteome profiles upon γ-radiation in recombinant Anabaena strains, AnpAM (vector-control) and AnlexA + (LexA-overexpressing), revealed 41 differentially accumulated proteins, corresponding to 29 distinct proteins. LexA was found to be involved in the regulation of 27 of the corresponding genes based on the presence of AnLexA-Box, EMSA, and/or qRT-PCR studies. The majority of the regulated genes were found to be involved in C-assimilation either through photosynthesis or C-catabolism and oxidative stress alleviation. Photosynthesis, measured in terms of PSII photophysiological parameters and thylakoid membrane proteome was found to be affected by γ-radiation in both AnpAM and AnlexA + cells, with LexA affecting them even under control growth conditions. Thus, LexA functioned as one of the transcriptional regulators involved in modulating γ-radiation stress response in Anabaena. This study could pave the way for a deeper understanding of the regulation of γ-radiation-responsive genes in cyanobacteria at large., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)