Search

An isocratic reversed phase high-performance liquid chromatographic method with ultraviolet detection at 220 nm has been developed for the quantification of itopride hydrochloride in tablet dosage form. The quantification was carried out using C8 column (250 mm × 4.6 mm), 5-μm particle size SS column. The mobile phase comprised of two solvents (Solvent A: buffer 1.4 mL ortho-phosphoric acid adjusted to pH 3.0 with triethyl amine and Solvent B: acetonitrile). The ratio of Solvent A: Solvent B was 75:25 v/v. The flow rate was 1.0 mL-1 with UV detection at 220 nm. The method has been validated and proved to be robust. The calibration curve was linear in the concentration range of 80-120% with coefficient of correlation 0.9995. The percentage recovery for itopride HCl was 100.01%. The proposed method was validated for its selectivity, linearity, accuracy, and precision. The method was found to be suitable for the quality control of itopride HCl in tablet dosage formulation. [ABSTRACT FROM AUTHOR]

The antimicrobial activity of different extracts from roots of Aristolochia bracteata retz was tested against Staphylococcus aureus, Escherichia coli, Salmonella typhi and Salmonella typhimurium which are pathogenic bacteria that cause gastrointestinal disorders. The root of A. bracteata retz was extracted with various solvents (petroleum ether, hexane, chloroform, ethyl acetate, methanol and water). The extracts were subjected to microbiological tests. Methanol was found to show more activity than other extracts. So the methanol extract was subjected for isolation. The isolated compound was confirmed by comparing with the standard aristolochic acid I. By using the same extract, the antioxidant activity was also tested by DPPH and Nitric oxide (NO) methods against free radicals which were present. More inhibition of the free radicals was observed in the DPPH method than NO method. [ABSTRACT FROM AUTHOR]