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18 results on '"Raipuria, Ritesh Kumar"'

5. Isolation, Characterization, and Expression Analysis of NAC Transcription Factor from Andrographis paniculata (Burm. f.) Nees and Their Role in Andrographolide Production

Author

Kumar, Ramesh, primary, Kumar, Chavlesh, additional, Roy Choudhury, Debjani, additional, Ranjan, Aashish, additional, Raipuria, Ritesh Kumar, additional, Dubey, Kaushik Kumar Dhar, additional, Mishra, Ayushi, additional, Kumar, Chetan, additional, Manzoor, Malik Muzafar, additional, Kumar, Ashok, additional, Kumari, Abha, additional, Singh, Kuldeep, additional, Singh, Gyanendra Pratap, additional, and Singh, Rakesh, additional

Published
2024
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13. Plant growth‐promoting rhizobacteria Shewanella putrefaciens and Cronobacter dublinensis enhance drought tolerance of pearl millet by modulating hormones and stress‐responsive genes

Author

Manjunatha, Booragamakalapalli Subbarayappa, primary, Nivetha, Nagarajan, additional, Krishna, Gopinathan Kumar, additional, Elangovan, Allimuthu, additional, Pushkar, Suchitra, additional, Chandrashekar, Narayanappa, additional, Aggarwal, Chetana, additional, Asha, Arambam Devi, additional, Chinnusamy, Viswanathan, additional, Raipuria, Ritesh Kumar, additional, Watts, Anshul, additional, Bandeppa, Sonth, additional, Dukare, Ajinath Shridhar, additional, and Paul, Sangeeta, additional

Published
2022
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17. <italic>Arabidopsis thaliana DHS2</italic> (<italic>AT4G33510</italic>) gene promoter is highly wound responsive and requires a part of the first exon sequences for its function.

Author

Raipuria, Ritesh Kumar, Kumar, Vajinder, Guruprasad, Kadur Narayan, and Bhat, Shripad Ramachandra

Abstract

Wound-responsive promoters are critical for developing the next generation of pest-tolerant transgenics, which would defend host by producing defensive factors at the site of attack. As shikimate pathway genes are known to be induced upon wounding, we evaluated the promoter of Arabidopsis thaliana DHS2 gene, which encodes for the first enzyme of this pathway. Transcription start site mapping showed DHS2 transcript has 89 nucleotide long 5′ UTR and RT-PCR revealed constitutive expression of DHS2 gene. Promoter analysis in transgenic A. thaliana using uidA reporter gene revealed that a part of the first exon of DHS2 is essential for promoter function. Histochemical GUS expression studies showed DHS2 expression in all green parts with the least expression in seedling roots and pollens. DHS2 promoter was found to be highly responsive to wounding giving 2-4 log fold up regulation within 10 min of wounding. Elevated expression level remained stable for 2-3 h and returned to normal after 24 h. In silico search identified wound responsive cis elements within the first exon of the DHS2 gene further underscoring the role of coding sequences in wound response and promoter activity. This is the first report demonstrating that coding sequence of a gene is an essential part of its promoter. [ABSTRACT FROM AUTHOR]

Published
2018
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18. Development of genome-specific SSR markers for the identification of introgressed segments of Sinapis albain the Brassica junceabackground

Author

Singh, Kaushal Pratap, Kumari, Preetesh, Raipuria, Ritesh Kumar, and Rai, Pramod Kumar

Abstract

Sinapis albaL. (white mustard) is recognized for carrying host resistance against several biotic stresses including, Alternaria brassicae, which is responsible for blight disease in cultivated Brassica. However, another cultivated Brassicahas a dearth for genetic resistance for these stresses due to its narrow genetic base. Therefore, we performed introgression of the genomic regions of S. albainto backcross progenies of B. juncea+ S. albasomatic hybrids. These advanced generations with S. albachromosomal segments are named B. juncea-S. albaintrogression lines (ILs). In the present study, we developed the S. albagenome-specific microsatellites from the draft genome to track the S. albagenome introgressions and responsible regions for resistance to A. brassicae. For developing these SSR markers, the unique contigs of S. albadraft genome were identified through BLASTN with B. juncea, B. rapa, B. nigra, and B. oleraceareference genome assemblies, including mitochondrial and chloroplast genomes, and further used for marker development. Out of 403,423 contigs, we have identified 65,343 non-hit contigs of S. albathat yielded a total of 1231 genome-specific microsatellites, out of which 1107 were expected to produce a single allele upon amplification. Out of the total SSRs, 234 primer pairs were randomly picked from whole-genome and validated between B. junceaand S. albagenomes for their specificity. In the validation experiment, these markers gave a single amplicon into S. alba, while they did not amplify in B. junceagenome. Of these, 59 microsatellites were used to track S. albaintrogressions in 80 BC2F3lines. To the best of our knowledge, this is the first time that these two genetic resources are developed in the form of B. juncea-S. albaILs and S. alba-specific markers. Therefore, both the resources unlock a new avenue of Brassicabreeding for biotic and abiotic stresses along with quality traits.

Published
2022
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