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105 results on '"Rahman, Sabita Rezwana"'

9. A comprehensive immunoinformatic analysis of chitin deacetylase’s and MP88 for designing multi-epitope vaccines against Cryptococcus neoformans

Author

Khan, Md Abu Sayem, Miah, Md Ibrahim, and Rahman, Sabita Rezwana

Abstract

AbstractCryptococcus neoformanscauses life-threatening pneumonia and meningitis and is regarded as one of the leading killers of immunocompromised individuals. There is currently no vaccine against this pathogen. Recently, WHO placed it at the top among the critical priority groups in the fungal priority pathogens to accelerate the development of effective treatments. Numerous studies suggested the potential of subunit vaccines to overcome the challenges associated with live and inactivated whole-cell vaccines. Therefore, this study exploited integrated reverse vaccinology and immunoinformatic approach to construct and characterize multi-epitope vaccines targeting chitin deacetylases (Cda1, Cda2, Cda3) and MP88 of C. neoformans. 4 CTL, 8 HTL and 6 B cell epitopes were fused with different adjuvants and appropriate linkers to design two multi-epitope vaccines (VC1 and VC2). Both chimeric constructs were predicted to be highly antigenic, non-allergenic, non-toxic, soluble and had satisfactory physicochemical properties. Molecular docking and binding free energy calculation revealed strong binding interactions between vaccine constructs and human TLRs (TLR-2 and TLR-4). Classical MD Simulation and Normal mode analysis verified the stability of the vaccine-TLR complex in the biological environment. Codon adaptation, cloning and in silicoexpression suggested the efficient expression of recombinant vaccine proteins in E. coli. Both candidates also generated robust immune profiles comprising innate, adaptive and humoral immune responses. Taken together, experimental validations of our findings through extensive in vitroand in vivotesting might provide an effective vaccine for prophylactic control of C. neoformans.Communicated by Ramaswamy H. Sarma

Published
2024
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10. Shotgun metagenomic analysis reveals the emergence of plasmid-encoded mcr-5.1gene in hospital wastewater in Bangladesh

Author

Khan, Abu Sayem, Afrin, Sunjida, Ahmed, Firoz, and Rahman, Sabita Rezwana

Abstract

•First report on the detection of colistin resistance conferring mcr-5.1 in hospital wastewater in Bangladesh.•The plasmid encoded mcr-5.1 belonged to the bacteria from the Enterobacteriaceae family.•The mcr-5.1operon was embedded in a Tn3 transposon.

Published
2024
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15. Characterization of biofilm formation and multi-drug resistance among Pseudomonas aeruginosa isolated from hospital wastewater in Dhaka, Bangladesh.

Author

Sayem Khan, Md Abu, Islam, Zahidul, Shah, S. M. Tanjil, and Rahman, Sabita Rezwana

Subjects
QUORUM sensing, MULTIDRUG resistance, PSEUDOMONAS aeruginosa, BIOFILMS, SEWAGE, GENTIAN violet, ANTIBIOTIC residues
Abstract

Hospital wastewater has been identified as a hotspot for the emergence and transmission of multidrug-resistant (MDR) pathogens that present a serious threat to public health. Therefore, we investigated the current status of antibiotic resistance as well as the phenotypic and genotypic basis of biofilm formation in Pseudomonas aeruginosa from hospital wastewater in Dhaka, Bangladesh. The disc diffusion method and the crystal violet assay were performed to characterize antimicrobial resistance and biofilm formation, respectively. Biofilm and integron-associated genes were amplified by the polymerase chain reaction. Isolates exhibited varying degrees of resistance to different antibiotics, in which .80% of isolates showed sensitivity to meropenem, amikacin, and gentamicin. The results indicated that 93.82% of isolates were MDR and 71 out of 76 MDR isolates showed biofilm formation activities. We observed the high prevalence of biofilm-related genes, in which algDþpelFþpslDþ (82.7%) was found to be the prevalent biofilm genotypic pattern. Sixteen isolates (19.75%) possessed class 1 integron (int1) genes. However, statistical analysis revealed no significant association between biofilm formation and multidrug resistance (χ2 = 0.35, P = 0.55). Taken together, hospital wastewater in Dhaka city may act as a reservoir for MDR and biofilm-forming P. aeruginosa, and therefore, the adequate treatment of wastewater is recommended to reduce the occurrence of outbreaks. [ABSTRACT FROM AUTHOR]

Published
2024
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20. Exploring Microbial Diversity and Functional Potential along the Bay of Bengal Coastline in Bangladesh: Insights from Amplicon Sequencing and Shotgun Metagenomics

Author

Akter, Salma, primary, Rahman, M. Shaminur, additional, Ali, Hazrat, additional, Minch, Benjamin, additional, Mehzabin, Kaniz, additional, Siddique, Md. Moradul, additional, Galib, Syed Md., additional, Yasmin, Farida, additional, Azmuda, Nafisa, additional, Adnan, Nihad, additional, Hasan, Nur A, additional, Rahman, Sabita Rezwana, additional, Moniruzzaman, Mohammad, additional, and Ahmed, Md. Firoz, additional

Published
2023
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23. Genome Sequence of Genotype 1A Hepatovirus A Isolated from Plasma from a Haitian Child

Author

Alam, M. Mahbubul, Elbadry, Maha A., Loeb, Julia C., Stephenson, Caroline J., Louis, Rigan, Mavian, Carla, Charrel, Remi, Rahman, Sabita Rezwana, Morris, J. Glenn, and Lednicky, John A.

Subjects
Immunology and Microbiology (miscellaneous), Genetics, Molecular Biology
Abstract

Genotype 1A hepatovirus A was identified by quantitative reverse transcription-PCR and isolated from plasma from a Haitian child with acute undifferentiated febrile illness and malaise. The strain was most closely related to Brazilian strains, consistent with recognized patterns of virus movement in the Caribbean region.

Published
2022
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30. Antibody response to the first dose of AZD1222 vaccine in COVID-19 convalescent and uninfected individuals in Bangladesh

Author

Jamiruddin, Raeed, primary, Haq, Ahsanul, additional, Khondoker, Mohib Ullah, additional, Ali, Tamanna, additional, Ahmed, Firoz, additional, Khandker, Shahad Saif, additional, Jawad, Irfan, additional, Hossain, Rubel, additional, Ahmed, Sohel, additional, Rahman, Sabita Rezwana, additional, Mustafi, Mamun, additional, Kaitsuka, Taku, additional, Mie, Masayasu, additional, Tomizawa, Kazuhito, additional, Kobatake, Eiry, additional, Haque, Mainul, additional, and Adnan, Nihad, additional

Published
2021
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32. Draft genome analysis of a multidrug-resistant Pseudomonas aeruginosaCMPL223 from hospital wastewater in Dhaka, Bangladesh

Author

Khan, Md Abu Sayem, Nahid, Zahidul Islam, Miah, Md Ibrahim, and Rahman, Sabita Rezwana

Abstract

•An emerging ST664 clone of MDR Pseudomonas aeruginosawas found in hospital wastewater in Dhaka, Bangladesh.•This strong biofilm-forming isolate was resistant to 21 out of 23 tested antibiotics except colistin and imipenem.•Whole genome analysis revealed that this isolate carried different AMR genes (sul1, catB7, blaOXA-50, blaOXA-796, ant(4′)-IIb, crpP) and virulence markers including lasA, lasB, rhlI, plcH, exoS, exoT, toxA, and fptA.

Published
2022
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40. Antimicrobial, antioxidant, and cytotoxic activities of Bixa orellana Linn

Author

Ul Islam, S.M. Bakhtiar, Hossain, Khalid, Gomes, Isidore, Gomes, Donald J., Rahman, Sabita Rezwana, Rahman, Mohammad S., and Rashid, Mohammad A.

Subjects
Técnicas de Química Analítica, antioxidant capacity, Bixa orellana Linn, brine shrimp lethality bioassay, multidrug resistant (MDR) bacteria, total phenolic content, Farmacia, Plantas Medicinales
Abstract

Bixa orellana Linn., commonly known as "lipstick plant", is used in folk medicines to treat infections of microbial origin as well as coloring agents in food stuffs in the LDCs like Bangladesh. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the warm water extract of leaves of B. orellana were evaluated against 25 multidrug resistant (MDR) clinical isolates and 6 food-borne pathogens using the micro-dilution broth method modified to comply with the NCCLS standards. The total phenolic content and antioxidant capacity of warm water, ethanol, and methanol extracts of the seeds and leaves of B. orellana were also evaluated. The brine shrimp lethality assay was conducted to assess the toxicity of the extracts. Except Pseudomonas spp., all the MDR isolates and food-borne pathogens tested were susceptible to the warm water extract of the leaves. The MIC and MBC ranged between 8-256 μg/mL and 16 - 256 μg/mL, respectively. Among the test organisms, Streptococcus spp. and Shigella dysenteriae-1 MJ-84 showed highest susceptibility while Escherichia coli exhibited moderate susceptibility to warm water extract of the leaves. The highest total phenolic content (99.99 mg of GAE/g of extractives) and antioxidant capacity (IC50 value 13 μg/mL) were observed in ethanolic extract of seeds of B. orellana, whereas the IC50 of the reference standard BHT (tert-butyl-1-hydroxytoluene) was 59.2 μg/mL. On the other hand, in the brine shrimp lethality bioassay the methanolic extract of the seeds of B. orellana demonstrated strong cytotoxic activity with IC50 value of 19.3 μg/mL. These results suggest that the extracts of B. orellana possess bioactive compounds, Colegio de Farmacéuticos de la Provincia de Buenos Aires

Published
2011

42. Differences in lineage replacement dynamics of G1 and G2 rotavirus strains versus G9 strain over a period of 22years in Bangladesh

Author

Afrad, Mokibul Hassan, primary, Matthijnssens, Jelle, additional, Afroz, Syeda Farhana, additional, Rudra, Pranab, additional, Nahar, Lutfun, additional, Rahman, Rajibur, additional, Hossain, Mohammad Enayet, additional, Rahman, Sabita Rezwana, additional, Azim, Tasnim, additional, and Rahman, Mustafizur, additional

Published
2014
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