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Introduction: Hepatitis B Virus (HBV) infection remains a significant public health concern globally, necessitating accurate and timely diagnostic methods. Immunochromatographic Tests (ICTs) and Electrochemiluminescence Assays (ECLIAs) are widely used assays for HBV detection due to their rapidity and cost-effectiveness. However, their diagnostic performance should be evaluated to ascertain their reliability. Aim: To detect the presence of Hepatitis B Surface Antigen (HBsAg) in the selected samples using ICT and ECLIA and to compare it with HBV Deoxyribonucleic Acid (DNA) using a molecular assay. Materials and Methods: A cross-sectional study was done with serum samples collected from patients visiting the hospital over a period of six months with prior ethical clearance. Serum samples were obtained from 57 patients suspected of HBV infection. The results of ICT, ECLIA, and HBV DNA viral load (by Polymerase Chain Reaction (PCR)) were cross-tabulated and assessed for differences in diagnostic sensitivity. The positivity and correlation of the ICT and ECLIA with PCR were estimated. All statistical analyses were performed using the R programming language. Results: Out of 57 samples, 53 (92.98%) tested positive in the ICT card test, and 54 (94.74%) were positive in the ECLIA method. McNemar’s test showed that the sensitivity of ICT and ECLIA differed significantly compared to HBV DNA PCR. There was a significant positive correlation between ECLIA and HBV-DNA PCR (Spearman correlation, r-value=0.28, p-value=0.035). Conclusion: The findings suggest that in settings where accurate diagnosis is critical, particularly for screening and monitoring treatment efficacy, molecular assays remain the preferred choice despite their higher cost and complexity. However, in resource-limited settings, ECLIAs can still play a valuable role in HBV screening programs.

Tolerance design has been identified as one of the key areas to realize the objective of producing quality goods at affordable cost. The impact of tolerance on the cost of manufacture for various metal-removal processes are shown by cost models. These cost models are in turn used in tolerance design to arrive at the relative cost of manufacture to various tolerances. Most of the cost models available untill recently were based on data from industries in the USA. However, Gunasekaran et al. (Gunasekaran, K., Neelakrishnan, S., Ragu, K. and Mohanram, P.V., Influence of tolerance on cost for various manufacturing processes, in National Conference on CAGQ - 2004, Thiagarajar College of Engineering, Madurai, India, 2004) presented the relative cost of tightening manufacture in Indian industries for attaining various tolerance values in Indian rupees and the results have been presented in graphical format. These formats are useful in determining the cost involved when tolerance synthesis methods are used to allocate tolerances to parts. In order to have an understanding of the costs involved to manufacture to a specific tolerance among the job shop-type and organized sector found in Indian industry, the cost versus tolerance data presented by Gunasekaran et al. (job-shop industry) have been compared with further data gathered by the present authors. In addition, the comparison includes the cost curves presented by Chase et al. (Chase, K.W., Greenwood, W.H., Loosli, B.G. and Hauglund, L.F., Least cost tolerance allocation for mechanical assemblies with automated process selection, J. Manuf. Rev. ASME, 1989, 2(4), 49-59). This is especially useful in analysing the differences in sourcing from a job shop versus the organized sector. In addition, the results that are displayed graphically are also compared with the cost models that already exist. In addition, one of the benefits of the cost curves is illustrated with a case study of a simple component. [ABSTRACT FROM AUTHOR]

Axial play between the impeller and its casing is one of the critical dimensions in a multistage submersible pump assembly. The axial play is introduced in the assembly using a coupling that connects the motor and pump sub assemblies. The length of the coupling could not be standardized by the industry where this analysis was conducted. Tolerance analysis is conducted on the pump subassembly to identify the causes of variation of coupling length. It has been found that there are n possible tolerance loops in a n stage pump. Since the tolerance design is to be conducted for the worst case, the assembly condition that yields the largest tolerance loop has been considered for the analysis. The methodology currently adopted by industry to overcome the problem of variation of coupling length involves machining operation during the assembly stage. It results in unreasonably longer assembly time. The concept of selective assembly is suggested in this paper to overcome the problem of coupling length variation. [ABSTRACT FROM AUTHOR]

Current progress on the mechanism and substrate recognition by sterol methyl transferase (SMT), the role of mechanism-based inactivators, other inhibitors of SMT action to probe catalysis and phytosterol synthesis is reported. SMT is a membrane-bound enzyme which catalyzes the coupled C-methylation-deprotonation reaction of sterol acceptor molecules generating the 24-alkyl sterol side chains of fungal ergosterol and plant sitosterol. This C-methylation step can be rate-limiting in the post-lanosterol (fungal) or post-cycloartenol (plant) pathways. A series of sterol analogs designed to impair SMT activity irreversibly have provided deep insight into the C-methylation reaction and topography of the SMT active site and as reviewed provide leads for the development of antifungal agents.

Background: Dental education in India predominantly relies on traditional lecture-based learning (LBL), which may hinder student engagement and learning outcomes. To address these limitations, innovative learning methodologies, such as spaced repetition learning (SRL), are imperative. SRL prioritizes active recall and can enhance long-term knowledge retention. This study aims to assess the effectiveness of SRL delivered through a mobile flashcard application, in enhancing knowledge retention among dental undergraduates., Methods: This single-blind randomized controlled trial (CTRI/2023/10/059347), conducted in Belagavi, India, involved 90 dental students who were equally distributed into control (LBL) and test (lecture followed by SRL demonstration) groups after randomization. Rigorous expert review ensured the quality of PowerPoint presentation and mobile flashcard contents. Knowledge assessments were conducted at baseline, first, and third months using a validated and reliable questionnaire. A perception survey on learning techniques was administered after the first month. Analysis methods included descriptive analysis, Pearson's chi-square test, independent t-test, and repeated measures ANOVA with Bonferroni's post hoc test., Results: The pre- and post-intervention knowledge showed no significant differences, but the SRL group exhibited significantly higher retention at both first month (p ≤ 0.001) and third months (p ≤ 0.001) than the LBL group. Repeated measures ANOVA revealed significant pairwise differences in mean knowledge scores in SRL group. Students had significantly favorable perception toward SRL than LBL group., Conclusion: SRL delivered through mobile flashcards significantly enhances knowledge retention compared to LBL among dental students. Positive student perceptions support SRL's integration into dental curricula, with implications for improving knowledge retention among them., (© 2024 American Dental Education Association.)

Objectives: Achyranthes aspera (Apamarga) and Trachyspermum ammi (Ajwain) have been used in many clinical conditions, and it displays valuable properties as an alternative to Chlorhexidine (CHX) in the management of gingivitis. Therefore, this study aims to assess the effect of Achyranthes aspera and Trachyspermum ammi (AA + TA) based herbal mouthwash, 0.2 % CHX, and placebo mouthwash on gingival health, plaque control and antibacterial activity against specific periodontal pathogens ( Porphyromonas gingivalis and Tannerella forsythia ) using quantitative real-time PCR (RT-PCR)., Methods: This was a randomized controlled non-inferiority trial involving 108 children with plaque-induced gingivitis who were randomly assigned to three groups of 36 children each: Group A, AA + TA mouthwash; Group B, CHX mouthwash; and Group C, placebo mouthwash. Gingival index and plaque index were recorded at baseline, 7 th and 21 st day. RT-PCR was employed to determine the bacterial counts of each plaque sample at baseline and after 21 days., Results: All three groups exhibited a gradual and significant reduction in both gingival and plaque scores from baseline to days 7 and 21. However, the placebo group did not demonstrate a significant difference in scores between days 7 and 21. Furthermore, a significant reduction in bacterial counts of P. gingivalis and T. forsythia was observed in the groups receiving CHX and AA + TA mouthwash after 21 days of intervention compared to the placebo group., Conclusion: AA + TA mouthwash demonstrated non-inferiority in anti-gingivitis and anti-plaque properties compared to CHX, suggesting its potential suitability as an alternative to CHX when used in conjunction with mechanical plaque control measures., (© 2024 The Authors.)

Background: Occupation significantly influences oral health, with factors like the work environment, stress levels, access to dental care, and job-related habits playing crucial roles. The oral health of construction workers, especially migrant workers, is a noteworthy concern. Understanding the oral health of this population is crucial for enhancing their quality of life through various means. This study aimed to investigate the prevalence of dental caries, oral hygiene status, and deleterious habits in this occupational group of Belagavi district, Karnataka., Materials and Methods: Study design was cross-sectional in nature. Before commencement of the study a pilot study was conducted. Multi-stage random sampling technique was employed, and 610 participants were recruited for the study. Trained and calibrated examiners recorded WHO dentition status and treatment needs (2013) and Oral Hygiene Index Simplified (OHI-S). Collected data was analyzed using descriptive analysis, chi-square, one-way ANOVA, and multiple linear regression analysis., Results: The prevalence of dental caries among construction workers was significantly high (81%), and poor oral hygiene was observed among 36.9% of them. The prevalence of smoking, the tobacco chewing habit, and alcohol consumption among the construction workers was found to be 21.6%, 59.9%, and 37.3%, respectively. The dependence of OHI-S and DMFT on predictors (age, gender and deleterious habits) was found to be 21.5% and 39.6%, respectively., Conclusions: Migrant construction workers in Belagavi had a high caries prevalence, poor oral hygiene status, and a high prevalence of deleterious habits such as tobacco use. These results emphasize the necessity of awareness and dental health education programs to improve the oral health of construction workers., Competing Interests: There was no conflict of interests associated with this original research article. IRB approval: Obtained from KLE V.K Institute of Dental Sciences Institutional Review Board., (©2024 Pacini Editore SRL, Pisa, Italy.)

Background:  The National Dental Commission Bill of 2023 introduced the National Exit Test (NExT) as a common final examination for all dental graduates in India, granting them the license to practice. This study evaluated dental undergraduates' awareness and perceptions of the newly proposed NExT., Methods:  A self-administered questionnaire in English comprising 23 close-ended questions was used to assess the awareness and perception of the students. A pilot study was conducted to determine the sample size, and 510 students were selected using a simple random sampling technique. The survey was administered to students ranging from 1st to 4th-year undergraduates and interns from two dental colleges in the Belagavi District, India. The questionnaire demonstrated good reliability (Cronbach's alpha coefficient = 0.86) and a content validity ratio 0.82., Results:  Interns had the highest mean awareness (39.56 ± 8.99) and perception (40.87 ± 5.56) scores, whereas first-year students had the lowest, with statistically significant differences among the groups (p ≤ 0.001). Although 81% of students were aware of NExT in India, only 17.3% found it student-friendly. A positive correlation was seen between the perception and awareness scores (r = + 0.242; p ≤ 0.001). The dependence of awareness and perception scores on predictors such as age, gender, and year of study were 16.7% and 15.3%, respectively., Conclusion:  Interns displayed a positive perception and higher awareness of NExT, whereas first and second-year dental students exhibited lower awareness and apprehensive perceptions. The introduction of NExT promises to enhance the overall quality of dental education on a national scale by providing high-quality care to patients., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2024, Santhosh et al.)