33 results on '"Radomska D"'
Search Results
2. Rhodiola rosea L. (Roseroot): In Vitro Regeneration and the Biological Activity of Roots
- Author
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Furmanowa, M., primary, Oledzka, H., additional, Michalska, M., additional, Sokolnicka, I., additional, and Radomska, D., additional
- Published
- 1995
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3. High Resistivity AlGaAs Grown by Low Temperature MBE
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Radomska, D., primary, Ratajczak, J., additional, Regiński, K., additional, and Bugajski, M., additional
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- 1998
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4. Quasi-Molecule Model of the Spectra of Molecules in Polar Solution in Case of Time Dependent Fluorescence Shifts
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Bączyński, A., primary, Marszałek, T., additional, Radomska, D., additional, Targowski, P., additional, and Ziętek, B., additional
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- 1992
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5. LT GaAs semi-insulating layers applied in vertical-cavity surface-emitting lasers.
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Reginski, K., Malag, A., Radomska, D., and Bugajski, M.
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- 1996
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6. Franck-Condon States of Coumarins in Alcoholic Solutions
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Bączyński, A., primary, Targowski, P., additional, Ziętek, B., additional, and Radomska, D., additional
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- 1990
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7. Fluorescence Spectra and Determination of the Energy of O-O Transitions of Coumarins in Liquid Solutions
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Bączyński, A., primary, Targowski, P., additional, Ziętek, B., additional, and Radomska, D., additional
- Published
- 1990
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8. Natural cell-mediated cytotoxicity against syngeneic rat chondrocytes originating from different types of cartilage.
- Author
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Radomska, D. M., Osiecka, A., and Malejczyk, J.
- Subjects
CELL-mediated cytotoxicity ,CARTILAGE cells ,CARTILAGE ,LABORATORY rats ,KILLER cells ,CELL death - Abstract
Summary Natural anti-chondrocyte cytotoxicity of normal rat splenocytes, peritoneal cells and thymocytes was tested by means of
51 Cr-release assay. Chondrocytes derived from epiphyseal, costal, nasal, and auricular cartilages were used as target cells. In some experiments, erythroleukaemic K-562 cells, known as typical natural killer cell targets, were also used. ALL types of chondrocytes were lysed equally well by splenocytes. Peritoneal cells exerted a low cytotoxic effect, whilst very low, almost negligible, cytotoxicity was noted with thymocytes. Negative selection with antibodies and complement showed that spleen-derived anti-chondrocyte effector cells are endowed with surface ganglioside asialo-GMI. A similar result was obtained in parallel experiments with K-562 cells. Moreover, 'cold' target experiments demonstrated that the release of51 Cr from the labelled chondrocytes could he inhibited by addition of unlabelled chondrocytes and K-562 cells. [ABSTRACT FROM AUTHOR]- Published
- 1989
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9. Cellular components of the bronchoalveolar lavage correlate with lung function impairment and extrapulmonary involvement markers in active sarcoidosis
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Chorostowska-Wynimko, J., Leśniewska-Radomska, D., Krychniak-Soszka, A., Remiszewski, P., Szopiński, J., Stefan Wesołowski, Kuś, J., and Skopińska-Rózewska, E.
- Subjects
Adult ,CD4-Positive T-Lymphocytes ,Male ,Sarcoidosis ,Splenomegaly ,CD4-CD8 Ratio ,Humans ,Female ,Lymphocytes ,CD8-Positive T-Lymphocytes ,Bronchoalveolar Lavage Fluid ,Lung ,Spleen - Abstract
Sarcoidosis is a chronic inflammatory multiorgan disease of unknown origin. Our previous study demonstrated a significant correlation between the relative count of non CD4(+), non CD8(+) lymphocytes in bronchoalveolar lavage of active sarcoidosis patients and proangiogenic activity of BAL homogenates. The aim of the present study was to evaluate in a group of 40 patients with active sarcoidosis the possible relationship between the intensity of alveolitis, particularly the non CD4(+), non CD8(+) lymphocyte subset, and other parameters characterizing the level of pulmonary (lung function tests) and extrapulmonary (spleen longitudinal dimension) disease activity. We found that the relative count of non CD4(+), non CD8(+) lymphocytes in BAL correlated positively with spleen size (r=0.50, P0.01) and negatively with static compliance (r=0.43, P0.05). We concluded that the lymphocytes belonging to the non CD4(+)non CD8(+) subset participate in the inflammatory process in sarcoidosis. However, more detailed phenotypic and functional characteristics of this cellular population are needed.
10. Low temperature MBE grown GaAs applied in vertical-cavity surface-emitting lasers
- Author
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Reginski, K., Malag, A., Radomska, D., Katcki, J., Jan Muszalski, Bugajski, M., and Strupinski, W.
11. Short Communication: Novel Di- and Triselenoesters as Effective Therapeutic Agents Inhibiting Multidrug Resistance Proteins in Breast Cancer Cells.
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Radomska D, Czarnomysy R, Marciniec K, Nowakowska J, Domínguez-Álvarez E, and Bielawski K
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- Humans, Female, Drug Resistance, Neoplasm drug effects, Cell Line, Tumor, Antineoplastic Agents pharmacology, Antineoplastic Agents chemistry, Organoselenium Compounds pharmacology, Organoselenium Compounds chemistry, Drug Resistance, Multiple drug effects, ATP Binding Cassette Transporter, Subfamily G, Member 2 metabolism, ATP Binding Cassette Transporter, Subfamily G, Member 2 antagonists & inhibitors, MCF-7 Cells, Neoplasm Proteins metabolism, Neoplasm Proteins antagonists & inhibitors, Molecular Docking Simulation, Multidrug Resistance-Associated Proteins metabolism, Multidrug Resistance-Associated Proteins antagonists & inhibitors, Esters pharmacology, Esters chemistry, ATP Binding Cassette Transporter, Subfamily B metabolism, ATP Binding Cassette Transporter, Subfamily B antagonists & inhibitors, Breast Neoplasms drug therapy, Breast Neoplasms metabolism, Breast Neoplasms pathology
- Abstract
Breast cancer has the highest incidence rate among all malignancies worldwide. Its high mortality is mainly related to the occurrence of multidrug resistance, which significantly limits therapeutic options. In this regard, there is an urgent need to develop compounds that would overcome this phenomenon. There are few reports in the literature that selenium compounds can modulate the activity of P-glycoprotein (MDR1). Therefore, we performed in silico studies and evaluated the effects of the novel selenoesters EDAG-1 and EDAG-8 on BCRP, MDR1, and MRP1 resistance proteins in MCF-7 and MDA-MB-231 breast cancer cells. The cytometric analysis showed that the tested compounds (especially EDAG-8) are inhibitors of BCRP, MDR1, and MRP1 efflux pumps (more potent than the reference compounds-novobiocin, verapamil, and MK-571). An in silico study correlates with these results, suggesting that the compound with the lowest binding energy to these transporters (EDAG-8) has a more favorable spatial structure affecting its anticancer activity, making it a promising candidate in the development of a novel anticancer agent for future breast cancer therapy.
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- 2024
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12. Recent Development of Fluoroquinolone Derivatives as Anticancer Agents.
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Nowakowska J, Radomska D, Czarnomysy R, and Marciniec K
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- Humans, Structure-Activity Relationship, Neoplasms drug therapy, Animals, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Drug Repositioning, Cell Proliferation drug effects, Fluoroquinolones chemistry, Fluoroquinolones pharmacology, Antineoplastic Agents pharmacology, Antineoplastic Agents chemistry, Antineoplastic Agents chemical synthesis
- Abstract
Cancer is the second leading cause of death in the world following cardiovascular disease. Its treatment, including radiation therapy and surgical removal of the tumour, is based on pharmacotherapy, which prompts a constant search for new and more effective drugs. There are high costs associated with designing, synthesising, and marketing new substances. Drug repositioning is an attractive solution. Fluoroquinolones make up a group of synthetic antibiotics with a broad spectrum of activity in bacterial diseases. Moreover, those compounds are of particular interest to researchers as a result of reports of their antiproliferative effects on the cells of the most lethal cancers. This article presents the current progress in the development of new fluoroquinolone derivatives with potential anticancer and cytotoxic activity, as well as structure-activity relationships, along with possible directions for further development.
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- 2024
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13. Di- and Triselenoesters-Promising Drug Candidates for the Future Therapy of Triple-Negative Breast Cancer.
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Radomska D, Czarnomysy R, Szymanowska A, Radomski D, Chalecka M, Surazynski A, Domínguez-Álvarez E, Bielawska A, and Bielawski K
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- Humans, Female, Cell Line, Tumor, Membrane Potential, Mitochondrial drug effects, Organoselenium Compounds pharmacology, Organoselenium Compounds therapeutic use, Organoselenium Compounds chemistry, Cell Survival drug effects, Esters chemistry, Esters pharmacology, MCF-7 Cells, Apoptosis drug effects, Cell Proliferation drug effects, Autophagy drug effects, Antineoplastic Agents pharmacology, Antineoplastic Agents chemistry, Antineoplastic Agents therapeutic use, Triple Negative Breast Neoplasms drug therapy, Triple Negative Breast Neoplasms metabolism, Triple Negative Breast Neoplasms pathology
- Abstract
Breast cancer is a major malignancy among women, characterized by a high mortality rate. The available literature evidence indicates that selenium, as a trace element, has chemopreventive properties against many types of cancer; as such, compounds containing it in their structure may potentially exhibit anticancer activity. Accordingly, we have undertaken a study to evaluate the effects of novel selenoesters (EDAG-1, -7, -8, -10) on MCF-7 and MDA-MB-231 breast cancer cells. Our analysis included investigations of cell proliferation and viability as well as cytometric determinations of apoptosis/autophagy induction, changes in mitochondrial membrane polarity (ΔΨ
m ), caspase 3/7, 8, and 9 activities, and Bax, Bcl-2, p53, Akt, AMPK, and LC3A/B proteins. The obtained data revealed that the tested derivatives are highly cytotoxic and inhibit cell proliferation even at nanomolar doses (0.41-0.79 µM). Importantly, their strong proapoptotic properties (↑ caspase 3/7) are attributable to the effects on both the extrinsic (↑ caspase 8) and intrinsic (↓ ΔΨm and Bcl-2, ↑ Bax, p53, and caspase 9) pathways of apoptosis. Moreover, the tested compounds are autophagy activators (↓ Akt, ↑ autophagosomes and autolysosomes, AMPK, LC3A/B). In summary, the potent anticancer activity suggests that the tested compounds may be promising drug candidates for future breast cancer therapy.- Published
- 2024
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14. Evaluation of anticancer activity of novel platinum(II) bis(thiosemicarbazone) complex against breast cancer.
- Author
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Radomska D, Szewczyk-Roszczenko OK, Marciniec K, Książek M, Kusz J, Roszczenko P, Szymanowska A, Radomski D, Bielawski K, and Czarnomysy R
- Subjects
- Humans, Structure-Activity Relationship, Molecular Structure, Female, Dose-Response Relationship, Drug, Cell Line, Tumor, Organoplatinum Compounds pharmacology, Organoplatinum Compounds chemistry, Organoplatinum Compounds chemical synthesis, Coordination Complexes pharmacology, Coordination Complexes chemistry, Coordination Complexes chemical synthesis, Platinum chemistry, Platinum pharmacology, Autophagy drug effects, Antineoplastic Agents pharmacology, Antineoplastic Agents chemical synthesis, Antineoplastic Agents chemistry, Thiosemicarbazones pharmacology, Thiosemicarbazones chemistry, Thiosemicarbazones chemical synthesis, Drug Screening Assays, Antitumor, Breast Neoplasms drug therapy, Breast Neoplasms pathology, Apoptosis drug effects, Cell Proliferation drug effects
- Abstract
The study aims to synthesize a novel bis(thiosemicarbazone) derivative based on platinum (thioPt) and evaluate its anticancer properties against MFC-7 and MDA-MB-231 breast cancer cells. A new platinum complex was synthesised by reacting K
2 PtCl4 with 2,2'-(1,2-diphenylethane-1,2-diylidene)bis(hydrazine-1-carbothioamide) in ethanol in the presence of K2 CO3 . In the obtained complex, the platinum atom is coordinated by a conjugated system = N-NC-S-The structures of the new compound were characterised using NMR spectroscopy, HR MS, IR, and X-ray structural analysis. The obtained results of the cytotoxicity assay indicate that compound thioPt had potent anticancer activity (MCF-7: 61.03 ± 3.57 µM, MDA-MB-231: 60.05 ± 5.40 µM) with less toxicity against normal MCF-10A breast epithelial cells, even compared to the reference compound (cisplatin). In addition, subsequent experiments found that thioPt induces apoptosis through both an extrinsic (↑caspase 8 activity) and intrinsic (↓ΔΨm ) pathway, which ultimately leads to an increase in active caspase 3/7 levels. The induction of autophagy and levels of proteins involved in this process (LC3A/B and Beclin-1) were examined in MCF-7 and MDA-MB-231 breast cancer cells exposed to tested compounds (thio, thioPt, cisPt) at a concentration of 50 µM for 24 h. Based on these results, it can be concluded that thio and thioPt do not significantly affect the autophagy process. This demonstrates their superiority over cisplatin, which can stimulate cancer cell survival through its effect on stimulation of autophagy., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)- Published
- 2024
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15. The activity of pyrazolo[4,3- e ][1,2,4]triazine and pyrazolo[4,3- e ]tetrazolo[1,5- b ][1,2,4]triazine sulphonamide derivatives in monolayer and spheroid breast cancer cell cultures.
- Author
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Szymanowska A, Radomska D, Czarnomysy R, Mojzych M, Kotwica-Mojzych K, Bielawski K, and Bielawska A
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- Humans, Structure-Activity Relationship, Molecular Structure, Tumor Cells, Cultured, Pyrazoles pharmacology, Pyrazoles chemistry, Pyrazoles chemical synthesis, Female, Cell Line, Tumor, Spheroids, Cellular drug effects, Triazines pharmacology, Triazines chemistry, Triazines chemical synthesis, Antineoplastic Agents pharmacology, Antineoplastic Agents chemistry, Antineoplastic Agents chemical synthesis, Breast Neoplasms drug therapy, Breast Neoplasms pathology, Breast Neoplasms metabolism, Drug Screening Assays, Antitumor, Sulfonamides pharmacology, Sulfonamides chemistry, Sulfonamides chemical synthesis, Cell Proliferation drug effects, Apoptosis drug effects, Dose-Response Relationship, Drug
- Abstract
In the last decade, an increasing interest in compounds containing pyrazolo[4,3- e ][1,2,4]triazine moiety is observed. Therefore, the aim of the research was to synthesise a novel sulphonyl pyrazolo[4,3- e ][1,2,4]triazines ( 2a , 2b ) and pyrazolo[4,3- e ]tetrazolo[1,5- b ][1,2,4]triazine sulphonamide derivatives ( 3a , 3b ) to assess their anticancer activity. The MTT assay showed that 2a , 2b , 3a , 3b have stronger cytotoxic activity than cisplatin in both breast cancer cells (MCF-7 and MDA-MB-231) and exhibited weaker effect on normal breast cells (MCF-10A). The obtained results showed that the most active compound 3b increased apoptosis via caspase 9, caspase 8, and caspase 3/7. It is worth to note that compound 3b suppressed NF-κB expression and promoted p53, Bax, and ROS which play important role in activation of apoptosis. Moreover, our results confirmed that compound 3b triggers autophagy through increased formation of autophagosomes, expression of beclin-1 and mTOR inhibition. Thus, our study defines a possible mechanism underlying 3b -induced anti-cancer activity against breast cancer cell lines.
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- 2024
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16. Vaccinium uliginosum and Vaccinium myrtillus -Two Species-One Used as a Functional Food.
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Kopystecka A, Kozioł I, Radomska D, Bielawski K, Bielawska A, and Wujec M
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- Anthocyanins pharmacology, Functional Food, Fruit, Antioxidants pharmacology, Plant Extracts pharmacology, Vaccinium myrtillus, Blueberry Plants
- Abstract
Vaccinium uliginosum L. (commonly known as bog bilberry) and Vaccinium myrtillus L. (commonly known as bilberry) are species of the genus Vaccinium (family Ericaceae ). The red-purple-blue coloration of blueberries is attributed largely to the anthocyanins found in bilberries. Anthocyanins, known for their potent biological activity as antioxidants, have a significant involvement in the prophylaxis of cancer or other diseases, including those of metabolic origin. Bilberry is the most important economically wild berry in Northern Europe, and it is also extensively used in juice and food production. A review of the latest literature was performed to assess the composition and biological activity of V. uliginosum and V. myrtillus . Clinical studies confirm the benefits of V. uliginosum and V. myrtillus supplementation as part of a healthy diet. Because of their antioxidant, anti-inflammatory, anti-cancer, and apoptosis-reducing activity, both bog bilberries and bilberries can be used interchangeably as a dietary supplement with anti-free radical actions in the prevention of cancer diseases and cataracts, or as a component of sunscreen preparations.
- Published
- 2023
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17. Thiosemicarbazide Derivatives Targeting Human TopoIIα and IDO-1 as Small-Molecule Drug Candidates for Breast Cancer Treatment.
- Author
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Kaproń B, Czarnomysy R, Radomska D, Bielawski K, and Plech T
- Subjects
- Female, Humans, Apoptosis, ATP Binding Cassette Transporter, Subfamily G, Member 2, Cell Line, Tumor, Cell Proliferation, MCF-7 Cells, Neoplasm Proteins metabolism, Semicarbazides pharmacology, Antineoplastic Agents pharmacology, Antineoplastic Agents therapeutic use, Antineoplastic Agents chemistry, Breast Neoplasms drug therapy, Breast Neoplasms metabolism
- Abstract
In 2020, breast cancer became the most frequently diagnosed type of cancer, with nearly 2.3 million new cases diagnosed. However, with early diagnosis and proper treatment, breast cancer has a good prognosis. Here, we investigated the effect of thiosemicarbazide derivatives, previously identified as dual inhibitors targeting topoisomerase IIα and indoleamine-2,3-dioxygenase 1 (IDO 1), on two distinct types of breast cancer cells (MCF-7 and MDA-MB-231). The investigated compounds ( 1 - 3 ) selectively suppressed the growth of breast cancer cells and promoted apoptosis via caspase-8- and caspase-9-related pathways. Moreover, these compounds caused S-phase cell cycle arrest and dose-dependently inhibited the activity of ATP-binding cassette transporters (MDR1, MRP1/2 and BCRP) in MCF-7 and MDA-MB-231 cells. Additionally, following incubation with compound 1, an increased number of autophagic cells within both types of the investigated breast cancer cells was observed. During preliminary testing of ADME-Tox properties, the possible hemolytic activities of compounds 1 - 3 and their effects on specific cytochrome P450 enzymes were evaluated.
- Published
- 2023
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18. Novel Selenoesters as a Potential Tool in Triple-Negative Breast Cancer Treatment.
- Author
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Radomska D, Czarnomysy R, Szymanowska A, Radomski D, Domínguez-Álvarez E, Bielawska A, and Bielawski K
- Abstract
Disturbing cancer statistics, especially for breast cancer, are becoming a rationale for the development of new anticancer therapies. For the past several years, studies have been proving a greater role of selenium in the chemoprevention of many cancers than previously considered; hence, a trend to develop compounds containing this element as potential agents with anticancer activity has been set for some time. Therefore, our study aimed to evaluate the anticancer activity of novel selenoesters (EDA-71, E-NS-4) in MCF-7 and MDA-MB-231 human breast cancer cells. The assays evaluating proliferation and cell viability, and flow cytometer analysis of apoptosis/autophagy induction, changes in mitochondrial membrane potential, disruption of cell cycle phases, and protein activity of mTOR, NF-κB, cyclin E1/A2, and caspases 3/7, 8, 9, 10 were performed. The obtained results indicate that the tested selenoesters are highly cytotoxic and exhibit antiproliferative activity at low micromolar doses (<5 µM) compared with cisplatin. The most active compound—EDA-71—highly induces apoptosis, which proceeds via both pathways, as evidenced by the activation of all tested caspases. Furthermore, we observed the occurrence of autophagy (↓ mTOR levels) and cell cycle arrest in the S or G2/M phase (↓ cyclin E1, ↑ cyclin A2).
- Published
- 2022
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19. Platinum and Palladium Complexes as Promising Sources for Antitumor Treatments.
- Author
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Czarnomysy R, Radomska D, Szewczyk OK, Roszczenko P, and Bielawski K
- Subjects
- Animals, Antineoplastic Agents pharmacology, Antineoplastic Agents therapeutic use, Clinical Trials as Topic, Drug Synergism, Humans, Organoplatinum Compounds pharmacology, Organoplatinum Compounds therapeutic use, Antineoplastic Agents chemistry, Neoplasms drug therapy, Organoplatinum Compounds chemistry, Palladium chemistry
- Abstract
There is a need for new, safer, and more effective agents to treat cancer. Cytostatics that have transition metals at their core have attracted renewed interest from scientists. Researchers are attempting to use chemotherapeutics, such as cisplatin, in combination therapy (i.e., in order to enhance their effectiveness). Moreover, studies are being carried out to modify molecules, by developing them into multinuclear structures, linking different compounds to commonly used drugs, or encapsulating them in nanoparticles to improve pharmacokinetic parameters, and increase the selectivity of these drugs. Therefore, we attempted to organize recent drug findings that contain palladium and platinum atoms in their structures.
- Published
- 2021
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20. Selenium as a Bioactive Micronutrient in the Human Diet and Its Cancer Chemopreventive Activity.
- Author
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Radomska D, Czarnomysy R, Radomski D, Bielawska A, and Bielawski K
- Subjects
- Humans, COVID-19 metabolism, Diet, Neoplasms drug therapy, Neoplasms metabolism, SARS-CoV-2 metabolism, Selenium therapeutic use, Trace Elements therapeutic use, COVID-19 Drug Treatment
- Abstract
This review answers the question of why selenium is such an important trace element in the human diet. Daily dietary intake of selenium and its content in various food products is discussed in this paper, as well as the effects of its deficiency and excess in the body. Moreover, the biological activity of selenium, which it performs mainly through selenoproteins, is discussed. These specific proteins are responsible for thyroid hormone management, fertility, the aging process, and immunity, but their key role is to maintain a redox balance in cells. Furthermore, taking into account world news and the current SARS-CoV-2 virus pandemic, the impact of selenium on the course of COVID-19 is also discussed. Another worldwide problem is the number of new cancer cases and cancer-related mortality. Thus, the last part of the article discusses the impact of selenium on cancer risk based on clinical trials (including NPC and SELECT), systematic reviews, and meta-analyses. Additionally, this review discusses the possible mechanisms of selenium action that prevent cancer development.
- Published
- 2021
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21. Selenium Compounds as Novel Potential Anticancer Agents.
- Author
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Radomska D, Czarnomysy R, Radomski D, and Bielawski K
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- Animals, Antineoplastic Agents chemistry, Chemoprevention methods, Clinical Trials as Topic, Humans, Neoplasms metabolism, Neoplasms prevention & control, Selenium Compounds chemistry, Antineoplastic Agents therapeutic use, Neoplasms drug therapy, Selenium Compounds therapeutic use
- Abstract
The high number of new cancer incidences and the associated mortality continue to be alarming, leading to the search for new therapies that would be more effective and less burdensome for patients. As there is evidence that Se compounds can have chemopreventive activity, studies have begun to establish whether these compounds can also affect already existing cancers. This review aims to discuss the different classes of Se-containing compounds, both organic and inorganic, natural and synthetic, and the mechanisms and molecular targets of their anticancer activity. The chemical classes discussed in this paper include inorganic (selenite, selenate) and organic compounds, such as diselenides, selenides, selenoesters, methylseleninic acid, 1,2-benzisoselenazole-3[2H]-one and selenophene-based derivatives, as well as selenoamino acids and Selol.
- Published
- 2021
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22. Evaluation of the Anticancer Activities of Novel Transition Metal Complexes with Berenil and Nitroimidazole.
- Author
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Czarnomysy R, Radomska D, Muszyńska A, Hermanowicz JM, Prokop I, Bielawska A, and Bielawski K
- Subjects
- Animals, Apoptosis drug effects, Apoptosis genetics, Cell Line, Tumor, Cell Survival drug effects, Cisplatin pharmacology, Coordination Complexes chemical synthesis, Coordination Complexes chemistry, DNA drug effects, DNA metabolism, DNA Topoisomerases, Type II metabolism, Diminazene chemistry, Female, Gold pharmacology, Humans, Membrane Potential, Mitochondrial drug effects, Mitochondria drug effects, Mitochondria metabolism, Palladium pharmacology, Platinum pharmacology, Transition Elements chemistry, Xenograft Model Antitumor Assays, Zebrafish, Antineoplastic Agents pharmacology, Autophagosomes drug effects, Breast Neoplasms drug therapy, Coordination Complexes pharmacology, Diminazene analogs & derivatives, Nitroimidazoles chemistry, Organometallic Compounds pharmacology
- Abstract
Novel transition metal complexes (Au, Pd, Pt) with berenil and 2-(1-methyl-5-nitroimidazol-2-yl)ethanol were obtained through two-step synthesis. The cytotoxicity assay against MCF-7 and MDA-MB-231 breast cancer cells revealed that novel platinum and palladium complexes cause a reduction on the viability of MCF-7 and MDA-MB-231 breast cancer cells to a greater extent than cisplatin. The complexes showed lower cytotoxicity on normal MCF-10A human breast epithelial cells than on tumor cells. Furthermore, we observed that these complexes selectively concentrate in tumor cell mitochondria due to the characteristic for these cells increased membrane potential that may explain their increased proapoptotic activity. The activity of the synthesized compounds against topoisomerase type IIα and their increased impact on DNA defragmentation also were documented. The novel complexes also induced autophagosome changes and inhibited tumor growth in xenograft models (established using breast cancer cells).
- Published
- 2020
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23. Cellular phenotypes and spatio-temporal patterns of lymphatic vessel development in embryonic mouse hearts.
- Author
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Flaht A, Jankowska-Steifer E, Radomska DM, Madej M, Gula G, Kujawa M, and Ratajska A
- Subjects
- Animals, Embryo, Mammalian, Gene Expression Regulation, Developmental, Glycoproteins genetics, Glycoproteins metabolism, Homeodomain Proteins genetics, Homeodomain Proteins metabolism, Membrane Transport Proteins, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Models, Biological, Myocardium cytology, Myocardium immunology, Organogenesis genetics, Organogenesis physiology, Phenotype, Time Factors, Tumor Suppressor Proteins genetics, Tumor Suppressor Proteins metabolism, Body Patterning genetics, Body Patterning physiology, Heart embryology, Lymphangiogenesis genetics, Lymphangiogenesis physiology, Lymphatic Vessels cytology, Lymphatic Vessels embryology
- Abstract
Background: The origin of cardiac lymphatics from venous endothelial cells or from scattered lymphangioblasts has been discussed in the literature. We aimed to establish the stage when lymphatic vessels appear in the developing mouse heart, the location of the first lymphatics, and to define cellular phenotypes of growing lymphatics., Results: We found that scattered Lyve-1-positive cells located in the subepicardial area of developing heart expressed CD45, CD68, F4/80, or CD11b but not CD31. Prox-1(+)/Lyve-1(+) cellular cords or vessels were found to invade 12.5-13.5-dpc hearts via two routes: from the venous pole, i.e., dorsal atrioventricular sulcus, or on the dorsal atrial surface from mediastinum and from the arterial pole, i.e., along the great arteries. The Prox-1(+)/Lyve-1(+) vessels were located among the Prox-1(+)/Lyve-1(-) cords and among the scattered Prox-1(-)/Lyve-1(+) cells. The Prox-1(+)/Lyve-1(-) cellular cords/tubules dominate initially at the arterial pole whereas Lyve-1(+)/Prox-1(-) cellular cords/tubules dominate initially on the venous pole, i.e., dorsal atrioventricular sulcus. The Lyve-1(+)/CD45(+), Lyve-1(+)/CD11b(+), Lyve-1(+)/F4/80(+) and Lyve-1(+)/CD68(+) cells were subsequently found to be co-opted to the wall of the developing lymphatic vessels while gaining Flk-1., Conclusions: Lymphatic primordia exhibit different cellular phenotypes and different spatiotemporal pattern on the venous pole as compared with the arterial pole of the heart., (Copyright © 2012 Wiley Periodicals, Inc.)
- Published
- 2012
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24. Disturbed angiogenic activity in sera from obstructive sleep apnea patients.
- Author
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Chorostowska-Wynimko J, Radomska D, Pływaczewski R, Jończak L, Stepniewska A, Górecka D, and Skopińska-Rózewska E
- Subjects
- Adult, Aged, Angiogenic Proteins blood, Animals, Case-Control Studies, Cells, Cultured, Female, Humans, Interleukin-6 metabolism, Leptin metabolism, Male, Mice, Mice, Inbred BALB C, Middle Aged, Severity of Illness Index, Sleep Apnea, Obstructive blood, Sleep Apnea, Obstructive physiopathology, Tumor Necrosis Factor-alpha metabolism, Adipose Tissue metabolism, Angiogenic Proteins metabolism, Leukocytes, Mononuclear metabolism, Neovascularization, Physiologic, Skin blood supply, Sleep Apnea, Obstructive metabolism
- Abstract
It is increasingly recognized that obstructive sleep apnea (OSA) syndrome is a systematic rather than local disorder. There is also growing evidence that apart from the syndrome's major features: intermittent hypoxia and sleep fragmentation, functional activity of the immune system is altered in OSA patients, with several cytokines, such as tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) taking active part in sleep regulation. Little is known about the effects exerted by chronic intermittent hypoxia combined with persistent pro-inflammatory activity of the immune system on the vascular micro milieu in OSA. In this study we attempted to confirm the hypothesized imbalance between pro- and anti-angiogenic factors by evaluating direct and indirect angiogenic activity of OSA patients' sera in the in vivo serum-induced angiogenesis (SIA) and leukocyte-induced (LIA) assays, respectively, in mice. Both tests revealed significantly inhibited angiogenic activity of OSA patients' sera compared with healthy controls (P<0.001). Moreover, differences related to the subject's weight regarding in the mean number of newly-formed vessels were observed with a significantly greater inhibition in the normal-weighing apneic subjects than in the overweight or obese ones (P<0.01). The angiogenesis inhibition index was positively related to the serum IL-6 level (r=0.35; P<0.05) in the OSA group, but not to TNF-alpha, fasting serum leptin, or OSA syndrome severity as assessed by the AHI index. Our results demonstrate that OSA is accompanied by disturbed serum angiogenic activity, apparently resulting from an imbalance between pro- and anti-angiogenic factors, some of them being produced by the adipose tissue. The disordered angiogenic activity might be related to the pathophysiology of OSA and should be considered an important causative factor for the increased prevalence of cardiovascular diseases in OSA patients.
- Published
- 2005
25. Cellular components of the bronchoalveolar lavage correlate with lung function impairment and extrapulmonary involvement markers in active sarcoidosis.
- Author
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Chorostowska-Wynimko J, Leśniewska-Radomska D, Krychniak-Soszka A, Remiszewski P, Szopiński J, Wesołowski S, Kuś J, and Skopińska-Rózewska E
- Subjects
- Adult, Bronchoalveolar Lavage Fluid immunology, CD4-CD8 Ratio, CD4-Positive T-Lymphocytes immunology, CD4-Positive T-Lymphocytes pathology, CD8-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes pathology, Female, Humans, Lymphocytes immunology, Male, Sarcoidosis immunology, Sarcoidosis pathology, Spleen immunology, Spleen pathology, Splenomegaly immunology, Splenomegaly pathology, Bronchoalveolar Lavage Fluid cytology, Lung physiopathology, Lymphocytes pathology, Sarcoidosis physiopathology
- Abstract
Sarcoidosis is a chronic inflammatory multiorgan disease of unknown origin. Our previous study demonstrated a significant correlation between the relative count of non CD4(+), non CD8(+) lymphocytes in bronchoalveolar lavage of active sarcoidosis patients and proangiogenic activity of BAL homogenates. The aim of the present study was to evaluate in a group of 40 patients with active sarcoidosis the possible relationship between the intensity of alveolitis, particularly the non CD4(+), non CD8(+) lymphocyte subset, and other parameters characterizing the level of pulmonary (lung function tests) and extrapulmonary (spleen longitudinal dimension) disease activity. We found that the relative count of non CD4(+), non CD8(+) lymphocytes in BAL correlated positively with spleen size (r=0.50, P<0.01) and negatively with static compliance (r=0.43, P<0.05). We concluded that the lymphocytes belonging to the non CD4(+)non CD8(+) subset participate in the inflammatory process in sarcoidosis. However, more detailed phenotypic and functional characteristics of this cellular population are needed.
- Published
- 2004
26. [Influence of isoniazid on selected parameters of immunological response].
- Author
-
Demkow U, Zielonka TM, Radomska D, Chorostowska-Wynimko J, and Skopińska-Rózewska E
- Subjects
- Animals, Antibody Formation drug effects, Cell Movement drug effects, Dose-Response Relationship, Drug, Hemagglutination drug effects, Humans, Immunity, Cellular drug effects, Lupus Vulgaris chemically induced, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Middle Aged, Antigens, CD drug effects, Antitubercular Agents adverse effects, Cytokines drug effects, Isoniazid adverse effects
- Abstract
Isoniazid (INH), antituberculous drug with immunomodulatory properties, have been described as lupus-like syndrome inducer. The development of autoimmunological phenomena results from immunoregulatory disturbances. The goal of this work was to determine the influence of INH on selected parameters of the immune response in vivo and in vitro. In vivo (in B6AF1 mice) the influence of long-term treatment on primary humoral response and cellular response was evaluated. Drug dose was 25 mg/kg. In vitro (using peripheral blood of volunteers) the influence of INH on mitogen induced proliferation, metabolic activity of granulocytes and production of angiogenic cytokines by diverse subpopulation of mononuclear cells was examined. The concentrations tested were 0.5 mg/ml, 5 mg/ml and 50 mg/ml. No effect of INH could be demonstrated on the production anti-SRBC antibodies nor on the cellular response in mice. In vitro INH added to the cell cultures increased PHA and ConA stimulated proliferation. The chemiluminescence of human granulocytes increased in the presence of INH. Drug enhanced production of angiogenic cytokines by human lymphocytes CD4+ and suppressed angiogenic activity of CD8+ cells. The results suggest that INH has strong immunomodulatory properties which may explain its involvement in pathogenesis of lupus-like disease.
- Published
- 2000
27. [The influence of rifampicin on selected parameters of immunologic response].
- Author
-
Demkow U, Radomska D, Chorostowska-Wynimko J, and Skopińska-Rózewska E
- Subjects
- Animals, Blood microbiology, Granulocytes drug effects, Granulocytes immunology, Humans, In Vitro Techniques, Luminescent Measurements, Mice, Mice, Inbred BALB C, Neovascularization, Physiologic drug effects, Neovascularization, Physiologic immunology, Antibody Formation drug effects, Immunity, Cellular drug effects, Rifampin pharmacology
- Abstract
Rifampicin (RMP), antituberculous drug, has been controversially described for many years as an immunosuppressant. The goal of this work was to determine the influence of RMP on selected parameters of the immune response in vivo and in vitro. In vivo (in B6AF1 mice) the influence of long-term treatment on primary humoral response and cellular response was evaluated. Drug dose was 50 mg/kg. In vitro (using peripheral blood of volunteers) the influence of RMP on mitogen induced proliferation, metabolic activity of granulocytes and leukocyte induced angiogenesis by diverse subpopulation of mononuclear cells was examined. The concentrations tested were 7 and 70 micrograms/ml. RMP slightly stimulated production of anti-SRBC antibodies and suppressed cellular response in mice, decreased PHA and ConA induced proliferation in higher concentration and strongly inhibited chemiluminescence at concentration used. RMP inhibited also leukocytes induced angiogenesis.
- Published
- 1998
28. Band shape analysis of electronic spectra of polar dye solutions.
- Author
-
Baczyński A and Radomska D
- Abstract
A semiclassical theory of electronic spectra of polar dye solutions is presented and analytical expressions of the spectra are given. A quasi-molecular approach is applied and the quasi-molecule model of the spectra previously published is reanalyzed. It is assumed that a large-amplitude motion plays a key role in the broadening of the spectra of polar dye solutions. An energy-level diagram of a quasi-molecule, considered as a dye molecule with its nearest neighborhood, is presented. In addition, the energy of reorientation in going from a Franck-Condon to an equilibrated state is determined. The orientation energy is equal to that part of the excitation energy given by the difference of the excited Franck-Condon state of a quasi-molecule from that of its equilibrated excited state. It is shown that after excitation, not only is excess vibrational, but also part of the electrostatic interaction energy is transferred to the surroundings. This energy may be obtained directly from absorption and fluorescence spectra of polar dye solutions. Experimental verification is performed on several coumarin solutions. The mean value for the reorientation energy in this case is found to be 645 cm-1.
- Published
- 1995
- Full Text
- View/download PDF
29. [Hyperreactivity and immunity in tuberculosis].
- Author
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Demkow U and Radomska D
- Subjects
- Humans, Immunity, Cellular, Tumor Necrosis Factor-alpha immunology, Hypersensitivity, Delayed immunology, Tuberculosis immunology
- Published
- 1995
30. The effect of TPP, theophylline and theobromine on the angiogenic activity of mononuclear leucocytes obtained from diabetic patients with proliferative retinopathy.
- Author
-
Skopiński P, Zukowska M, Małkowska-Zwierz W, Radomska D, Retelska D, Kecik D, Domosławski M, Sobczyńska A, Pawińska M, and Skopińska-Rózewska E
- Subjects
- Adolescent, Adult, Animals, Child, Drug Combinations, Humans, Leukocytes, Mononuclear physiology, Mice, Mice, Inbred BALB C, Soil, Amino Acids pharmacology, Carbohydrates pharmacology, Diabetic Retinopathy pathology, Humic Substances pharmacology, Leukocytes, Mononuclear drug effects, Neovascularization, Pathologic etiology, Theobromine pharmacology, Theophylline pharmacology, Uronic Acids pharmacology
- Abstract
Tołpa Peat Preparation (TPP) administered in various doses does not influence the ability of mononuclear cells (MNC) obtained from diabetic patients with proliferative retinopathy to induce neovascularization response in H-LIA test. Methylxanthines (theophylline, theobromine) significantly decrease the angiogenic activity of these cells. The therapeutic value of these drugs in proliferative retinopathy should be evaluated.
- Published
- 1993
31. Effect of purinergic receptor antagonists suramin and theobromine on tumor-induced angiogenesis in BALB/c mice.
- Author
-
Gil M, Skopińska-Rózewska E, Radomska D, Demkow U, Skurzak H, Rochowska M, Beuth J, and Roszkowski K
- Subjects
- Animals, Carcinoma blood supply, Injections, Subcutaneous, Lung Neoplasms blood supply, Male, Mice, Mice, Inbred BALB C, Neoplasms, Experimental drug therapy, Tumor Cells, Cultured, Neoplasms, Experimental blood supply, Neovascularization, Pathologic drug therapy, Purinergic Antagonists, Suramin pharmacology, Theobromine pharmacology
- Abstract
The purinergic receptor antagonists suramin (SRN) and theobromine (TBR) were examined for their anti-angiogenic activity in BALB/c mice. SRN or TBR were subcutaneously administered to BALB/c mice in doses of 1-125 mg/kg body weight on days 0, 1 and 2 after intradermal inoculation of E14/W lung carcinoma cells. It was shown that SRN and TBR inhibited tumor-related angiogenesis. Accordingly, it may be suggested that purinoceptor antagonists may inhibit neovascularization in tumor growth and metastasis.
- Published
- 1993
32. Electronic spectra of dye solutions. I. The mirror image rule.
- Author
-
Bączyński A and Radomska D
- Abstract
The main assumptions of the quasi-molecule model of electronic spectra of liquid dye solutions are presented. The derived analytical expressions of the spectra account for the solvatochromic effects present in the solution. Based on the obtained spectral profiles of some coumarins in alcohol, new criteria for the validity of the mirror image rule are formulated.
- Published
- 1992
- Full Text
- View/download PDF
33. [Assessment of the value of stimulation from a preparation of Tołpa peat for treatment of trauma related osteomyelitis].
- Author
-
Wasikowski A, Syrówka P, Zabuska-Jabłońska K, Lipińska R, Radomska D, Demkow U, Skopińska-Rózewska E, and Roszkowski W
- Subjects
- Adolescent, Adult, Aged, Arm Injuries complications, Female, Humans, Leg Injuries complications, Male, Middle Aged, Osteomyelitis etiology, Treatment Outcome, Mud Therapy, Osteomyelitis therapy
- Abstract
Tołpa peat preparation at the dose of 5 mg per day was administered to 10 patients with chronic posttraumatic osteomyelitis for 6 weeks period of time. Good clinical result (ceasing of the inflammatory process) was found in 4 patients but 3 of them were concurrently treated surgically. Immunological examination (angiogenesis test and chemiluminescence test) performed before the onset and after completion of the treatment with Tołpa preparation did not reveal meaningful changes indicating immunomodulating action of the preparation on the course of posttraumatic osteomyelitis.
- Published
- 1992
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