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5 results on '"Rachel S. Quizon"'

1. Citrullination of a phage-displayed human peptidome library reveals the fine specificities of rheumatoid arthritis-associated autoantibodies

Author

Gabriel D. Román-Meléndez, Daniel R. Monaco, Janelle M. Montagne, Rachel S. Quizon, Maximilian F. Konig, Mekbib Astatke, Erika Darrah, and H. Benjamin Larman

Subjects
Phage ImmunoPrecipitation Sequencing, Citrullination, Peptidylarginine deiminase, Rheumatoid arthritis, Medicine, Medicine (General), R5-920
Abstract

Background: Post-translational modifications (PTMs) on proteins can be targeted by antibodies associated with autoimmunity. Despite a growing appreciation for their intrinsic role in disease, there is a lack of highly multiplexed serological assays to characterize the fine specificities of PTM-directed autoantibodies. Methods: In this study, we used the programmable phage display technology, Phage ImmunoPrecipitation Sequencing (PhIP-Seq), to profile rheumatoid arthritis (RA) associated anti-citrullinated protein antibody (ACPA) reactivities. Findings: Using both unmodified and peptidylarginine deiminase (PAD)-modified phage display libraries consisting of ~250,000 overlapping 90 amino acid peptide tiles spanning the human proteome, PTM PhIP-Seq robustly identified antibodies to citrulline-dependent epitopes. Interpretation: PTM PhIP-Seq was used to quantify key differences among RA patients, including PAD isoform specific ACPA profiles, and thus represents a powerful tool for proteome-scale antibody-binding analyses. Funding: This research is based upon work supported in part by the Office of the Director of National Intelligence (ODNI), Intelligence Advanced Research Projects Activity (IARPA). The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of ODNI, IARPA, or the US Government. The US Government is authorized to reproduce and distribute reprints for governmental purposes notwithstanding any copyright annotation therein. This study was made possible by a National Institute of General Medical Sciences (NIGMS) grant R01 GM136724 (HBL). MFK was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) grant T32AR048522. ED was supported by the Rheumatology Research Foundation.

Published
2021
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2. Citrullination of a phage displayed human peptidome library reveals the fine specificities of rheumatoid arthritis-associated autoantibodies

Author

H. Benjamin Larman, Maximilian F. Konig, Daniel R. Monaco, Mekbib Astatke, Gabriel D. Román-Meléndez, Rachel S. Quizon, Erika Darrah, and Janelle M. Montagne

Subjects
Phage display, biology, Immunoprecipitation, Human proteome project, biology.protein, Autoantibody, medicine, Citrullination, Computational biology, Antibody, medicine.disease_cause, Epitope, Autoimmunity
Abstract

Post-translational modifications (PTMs) on proteins can be targeted by antibodies associated with autoimmunity. Despite a growing appreciation for their intrinsic role in disease, there is a lack of highly multiplexed serological assays to characterize the fine specificities of PTM-directed autoantibodies. In this study, we used the programmable phage display technology, Phage ImmunoPrecipitation Sequencing (PhIP-Seq), to profile rheumatoid arthritis (RA) associated anti-citrullinated protein antibody (ACPA) reactivities. Using both an unmodified and peptidylarginine deiminases (PAD)-modified phage display library consisting of ~250,000 overlapping 90 amino acid peptide tiles spanning the human proteome, PTM PhIP-Seq robustly identifies antibodies to citrulline-dependent epitopes. PTM PhIP-Seq was used to quantify key differences among RA patients, including PAD isoform specific ACPA profiles, and thus represents a powerful tool for proteome-scale antibody-binding analyses.

Published
2021
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3. Improved Method for the Evaluation of Real-Time Biological Aerosol Detection Technologies

Author

Jason Quizon, Michael L. Wagner, Rachel S. Quizon, Neal Baker, Eric Van Gieson, Charles Kerechanin, Joshua L. Santarpia, Alex Proescher, Gerad House, Shanna Ratnesar-Shumate, Christopher Bare, and Kelly Brinkley

Subjects
Meteorology, Improved method, respiratory system, Atmospheric sciences, complex mixtures, Pollution, Aerosol, Bacillus thuringiensis kurstaki, Aerosol generator, Environmental Chemistry, Particle, Environmental science, General Materials Science, Bioaerosol
Abstract

There is a growing need to evaluate bioaerosol sensors under relevant operational conditions. New methods are needed that can mimic the temporal fluctuations of ambient aerosol backgrounds and present biological aerosol challenges in a way that simulates a plausible biological agent attack. The Dynamic Concentration Aerosol Generator was developed to address this need. The authors developed a series of aerosol challenges consisting of Bacillus thuringiensis kurstaki (Btk) spores in the presence of background aerosols using a newly developed ramp testing method. Using ramping style tests, 5-min Btk releases were overlaid on top of a background aerosol that fluctuated at varying rates. Background aerosol compositions for different tests were designed to simulate the types of aerosol in the ambient environment. Background aerosol concentration was varied between 7.0 × 103 and 1.5 × 104 particles per liter of air (ppL). Aerosol number concentrations of Btk for the challenges were approximately 2.5 × 103 ppL a...

Published
2011
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4. Rapid Characterization of Microorganisms by Mass Spectrometry

Author

Catherine Fenselau, Plamen Demirev, Franco Basile, Justin M. Hettick, Brett J. Green, Amanda D. Buskirk, James E. Slaven, Michael L. Kashon, Donald H. Beezhold, Vladimir Havlicek, Karel Lemr, Scott C. Russell, John R. Barr, Suzanne R. Kalb, James L. Pirkle, Clifton K. Fagerquist, Nathan J. Edwards, Colin Wynne, Avantika Dhabaria, Juaneka M. Hayes, Louis C. Anderson, J. Albert Schultz, Michael Ugarov, Thomas F. Egan, Ernest K. Lewis, Virginia Womack, Amina S. Woods, Shelley N. Jackson, Robert H. Hauge, Carter Kittrell, Steve Ripley, Kermit K. Murray, Matthias Frank, Eric E. Gard, Herbert J. Tobias, Kristl L. Adams, Michael J. Bogan, Keith R. Coffee, George R. Farquar, David P. Fergenson, Sue I. Martin, Maurice Pitesky, Vincent J. Riot, Abneesh Srivastava, Paul T. Steele, Audrey M. Williams, Berk Oktem, Appavu K. Sundaram, Jane Razumovskaya, Seshu K. Gudlavalleti, Thomas D. Saul, Vladimir M. Doroshenko, Nathan A. Hagan, Jeffrey S. Lin, Miquel D. Antoine, Timothy J. Cornish, Rachel S. Quizon, Bernard F. Collins, Andrew B. Feldman, Plamen A. Demir, Catherine Fenselau, Plamen Demirev, Franco Basile, Justin M. Hettick, Brett J. Green, Amanda D. Buskirk, James E. Slaven, Michael L. Kashon, Donald H. Beezhold, Vladimir Havlicek, Karel Lemr, Scott C. Russell, John R. Barr, Suzanne R. Kalb, James L. Pirkle, Clifton K. Fagerquist, Nathan J. Edwards, Colin Wynne, Avantika Dhabaria, Juaneka M. Hayes, Louis C. Anderson, J. Albert Schultz, Michael Ugarov, Thomas F. Egan, Ernest K. Lewis, Virginia Womack, Amina S. Woods, Shelley N. Jackson, Robert H. Hauge, Carter Kittrell, Steve Ripley, Kermit K. Murray, Matthias Frank, Eric E. Gard, Herbert J. Tobias, Kristl L. Adams, Michael J. Bogan, Keith R. Coffee, George R. Farquar, David P. Fergenson, Sue I. Martin, Maurice Pitesky, Vincent J. Riot, Abneesh Srivastava, Paul T. Steele, Audrey M. Williams, Berk Oktem, Appavu K. Sundaram, Jane Razumovskaya, Seshu K. Gudlavalleti, Thomas D. Saul, Vladimir M. Doroshenko, Nathan A. Hagan, Jeffrey S. Lin, Miquel D. Antoine, Timothy J. Cornish, Rachel S. Quizon, Bernard F. Collins, Andrew B. Feldman, and Plamen A. Demir

Subjects
Medical laboratory technology, Microorganisms--Identification, Mass spectrometry, Microbiological chemistry
Published
2011

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