1. Combined genetic and physical map of the complex genome of Agrobacterium tumefaciens.
- Author
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Goodner BW, Markelz BP, Flanagan MC, Crowell CB Jr, Racette JL, Schilling BA, Halfon LM, Mellors JS, and Grabowski G
- Subjects
- Chromosome Mapping, Chromosomes, Bacterial, Physical Chromosome Mapping, Agrobacterium tumefaciens genetics, Genome, Bacterial
- Abstract
A combined genetic and physical map of the Agrobacterium tumefaciens A348 (derivative of C58) genome was constructed to address the discrepancy between initial single-chromosome genetic maps and more recent physical mapping data supporting the presence of two nonhomologous chromosomes. The combined map confirms the two-chromosome genomic structure and the correspondence of the initial genetic maps to the circular chromosome. The linear chromosome is almost devoid of auxotrophic markers, which probably explains why it was missed by genetic mapping studies.
- Published
- 1999
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