1. Rab4b Promotes Cytolethal Distending Toxin from Glaesserella parasuis -Induced Cytotoxicity in PK-15 Cells.
- Author
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Zhang, Yiwen, Yang, Zhen, Dai, Ke, Hu, Bangdi, Xu, Shiyu, Wang, Yu, Lei, Li, Du, Senyan, Zhao, Qin, Huang, Xiaobo, Wu, Rui, Yan, Qigui, Wang, Yiping, Cao, Sanjie, and Wen, Yiping
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CYTOTOXINS , *CELL cycle , *ENDOSOMES , *GUANOSINE triphosphatase , *CELL lines - Abstract
Glaesserella parasuis cytolethal distending toxin (GpCDT) can induce cell cycle arrest and apoptosis. Our laboratory's previous work demonstrated that GTPase 4b (Rab4b) is a key host protein implicated in GpCDT-induced cytotoxicity. This study investigated the probable involvement of Rab4b in the process. Our study used CRISPR/Cas9 technology to create a Rab4b-knockout cell line. The results showed greater resistance to GpCDT-induced cell cytotoxicity. In contrast, forced Rab4b overexpression increased GpCDT-induced cytotoxicity. Further immunoprecipitation study reveals that GpCDT may bind with Rab4b. In PK-15 cells, GpCDT is transported to the early endosomes and late endosomes, while after knocking out Rab4b, GpCDT cannot be transported to the early endosome via vesicles. Rab4b appears essential for GpCDT-induced cytotoxicity in PK-15 cells. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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