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Abstract Background The Harris hawk is a bird of prey susceptible to traumatic injuries because it is useful for several purposes such as conservancy, biological control and falconry. Once received in rehabilitation centres or specialized clinics, it is necessary to provide proper analgesia. Objectives The aim of this study is to demonstrate the analgesic efficacy of tramadol in Harris hawks (PISADOL 50 PiSA Agropecuaria, S.A. de C.V. Calle 1 Norte, Manzana 2‐25 Parque Industrial Tula Atitalaquia, Hgo, México), by the assessment of nociceptive threshold. Methods A total of 24 adult Harris hawks were selected from a rehabilitation centre. The birds were randomly divided into four groups: control (saline solution), 5.0, 15.0 and 30.0 mg/kg of intramuscular tramadol. Nociception was produced with electrical stimuli of 9 V, applied in propatagial skin at 1, 5, 10, 20, 30, 45, 60, 90, 120, 180, 240, 300 and 360 min, assessing the nociceptive threshold and sedative effects produced by each treatment. Results No difference was observed between control and tramadol group 5 mg/kg. At 15 mg/kg, the pain threshold increased from 20 to 240 min, with minimal sedative effects. At 30 mg/kg, there was a marked increase in pain threshold from 10 to 300 min, and sedative effects like wing and head drooping for a period of 90 min. Conclusions Tramadol can be an analgesic alternative for Harris's hawks, as it decreases the response to painful stimuli in this species when administered by intramuscular route.

Abstract Background Blood biochemistry and reference intervals help to differentiate between healthy and ill dogs as well as to provide information for the prognosis, evaluation, and monitoring; however, these intervals are often obtained from adult animals. It is essential to understand that puppies and adults are physiologically different, which justifies the need to obtain age-specific biochemical reference intervals. The aim of this research was to assess the potential effect of age, sex, body size, and their interaction on routine biochemical analytes and physiological constants (body temperature, heart rate, and respiratory rate). To carry out the research, we selected 197 healthy dogs of both sexes and different body sizes (small, medium and large) classified by age: group I (4–8 wk), group II (9–24 wk), group III (25–52 wk), and group IV (> 52 wk). The biochemical analysis included the measurement of the enzymatic activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), and the concentrations of cholesterol, triglycerides, total proteins, albumin, globulins, glucose, urea, and creatinine. Statistical analyses used analysis of variance (ANOVA) and a general linear model (GLM), which allows the comparison of multiple factors at two or more levels (p 0.05). Conclusions Some biochemical components are influenced by age. For this reason, this manuscript contributes with additional data for the clinical interpretation of blood biochemical results in puppies.

Background. Propylene glycol has been used successfully since the 1950’s for him acute and prophylactic treatment of ketosis in dairy cows however; its use has been poorly evaluated in beef cattle and meat sheep. Objective. Evaluate the effects of different doses of propylene glycol on the productive parameters, blood metabolites and ruminal parameters in fattening lambs. Methodology. Twenty lambs 14.70 ±0.57 kg of weigh, 2 months old, males and Katahdin-Black Belly breed, were randomly assigned to one of four treatments, a control mixed ration and an experimental diet with three different levels of propylene glycol: 1.0, 1.5 and 2.0 g/20 kg live weight/day. The effects of propylene glycol on blood metabolites, ruminal and productive parameters were assessed. Results. Propylene glycol supplementation did not affect (P>0.05) glucose, total lipids, triglycerides and cholesterol concentration as well as increased (P0.05) on feed intake, weight gain and feed conversion ratio of lambs but carcass yield was improved without increasing the feeding cost of production. Implications. Propylene glycol is a viable option as an energy source in fattening lambs. Conclusion. Inclusion of PPG increased the pH and the population of ruminal protozoa, reduced the formation of ketone bodies and improved the carcass yield of lambs without increasing the meat production cost.

Los avestruces tienen capacidad para digerir la fibra de los alimentos, pero no se han realizado estudios sobre la utilidad de incluir ingredientes de uso común en México, en la formulación de raciones integrales. Con el objetivo de evaluar la digestibilidad aparente (DA) o proporción en que son retenidos los nutrientes de los alimentos, se formularon dietas con concentraciones crecientes de ensilaje de maíz (EM: A: 0.00, B: 10.0, C: 20.0 y D: 30.0 %). Todas tenían la misma concentración de proteína cruda (12.5 %), fibra (16.0 %) y energía metabolizable (2.6 Mcal/kg). Con un diseño de cuadro latino 4x4, cada dieta se suministró consecutivamente a cada uno de cuatro avestruces de 12 meses de edad, en etapa de finalización, género macho. Después de un periodo de tres días de acondicionamiento, durante siete días se colectaron muestras de las dietas y de las heces, se estimó DA de materia seca (DAMS) y de proteína (DAP) y digestibilidad de fibra detergente neutro (FDN) y detergente ácido (FDA). No se observaron diferencias significativas entre el consumo de alimento, incremento de peso y conversión alimenticia. Sin embargo, la inclusión de EM mejoró DAMS, DAP y FDA (P

La contaminación de productos agrícolas y pecuarios con aflatoxinas (AF) está distribuida mundialmente. Las AF son tóxicas, inmunodepresoras y carcinogénicas, pero en México es escasa la información sobre Aspergillus flavus, principal hongo que las produce. El objetivo fue caracterizar morfológica, molecular y aflatoxigénicamente aislados de A. flavus y cuantificar AF en pienso y en leche de vacas Holstein en Aguascalientes (México). Se seleccionó por conveniencia una unidad de producción lechera (2,749 vacas) y se recolectaron muestras mensuales (24 meses) de ingredientes alimenticios y ración total mezclada (n= 267), leche cruda (n= 288) y suelo agrícola (n= 40), las cuales se cultivaron (PDA) mediante vaciado en placa con diluciones seriadas. Los hongos se caracterizaron mediante MEB, TLC y vapores de amonio en agar coco; se secuenciaron los genes de calmodulina y regulador de la ruta biosintética de AF, así como la región de los espaciadores internos de la transcripción. Se cuantificaron AF en pienso con HPLC y en leche con ELISA. Se caracterizaron molecularmente 283 aislados fúngicos; 88 resultaron ser Aspergillus spp, de los que 5 fueron A. flavus con capacidad aflatoxigénica y uno no aflatoxigénico. El 99.3 % de las muestras de alimento y 39.9 % de las muestras de leche presentaron niveles detectables de AF (14.8 y 0.021 µg/kg). Las vacas ingirieron diariamente 621 µg de AF y eliminaron el 0.09 % como aflatoxina M1 en leche. Lo anterior sugiere que la ocurrencia A. flavus aflatoxigénico en el alimento de vacas lecheras conduce a una amplia contaminación por AF de las dietas y de la cadena alimenticia.

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Since its first patent (1897), commercial dry feed (CDF) for dogs has diversified its formulation to meet the nutritional needs of different breeds, age, or special conditions and establish a foundation for integration of these pets into urban lifestyles. The risk of aflatoxicosis in dogs has increased because the ingredients used to formulate CDF have also proliferated, making it difficult to ensure the quality required of each to achieve the safety of the entire CDF. This review contains a description of the fungi and aflatoxins detected in CDF and the ingredients commonly used for their formulation. The mechanisms of action and pathogenic effects of aflatoxins are outlined; as well as the clinical findings, and macroscopic and microscopic lesions found in aflatoxicosis in dogs. In addition, alternatives for diagnosis, treatment, and control of aflatoxins (AF) in CDF are analyzed, such as biomarkers of effect, improvement of blood coagulation, rate of elimination of AF, control of secondary infection, protection of gastric mucosa, reduction of oxidative stress, use of chemo-protectors, sequestrants, grain-free CDF, biocontrol, and maximum permitted limits, are also included.