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AbstractTwelve monoterpene indole glycoalkaloids, comprising of three new ones, 19-epi-rhynchophylloside A (1), 7-epi-rhynchophylloside A (2), and 7-epi-anthocephalusine A (3), were isolated from the hook-bearing branches of Uncaria rhynchophylla. The structures and absolute configurations of 1–3 were elucidated by analysis of MS, NMR, ECD, and single-crystal X-ray diffraction or TDDFT-ECD calculations. Glycoalkaloids 1 and 3 showed significant immunosuppressive activity against the proliferation of B lymphocyte induced by LPS with broad selective index. [ABSTRACT FROM AUTHOR]

Introduction: This study presents the first complete plastome (cpDNA) sequence of Psydrax latifolia, a member of the Rubiaceae family, which includes small trees, smooth shrubs, and occasionally lianas. This specimen was collected near the Red Sea coast in Jazan province, Saudi Arabia, specifically in the paleotropical Fifa Mountains. The research aimed to characterize and compare the plastome of Psydrax latifolia with other species in the Rubiaceae family to enhance our understanding of its evolutionary dynamics and phylogenetic relationships. Methods: The plastome of P. latifolia was sequenced and reconstructed using whole-genome next-generation sequencing (NGS) techniques. Comparative analyses were performed between the plastome of P. latifolia and 16 other species within the Rubiaceae family to identify genomic features and evolutionary patterns. The plastome structure, gene content, and codon usage were analyzed, with a focus on the Relative Synonymous Codon Usage (RSCU) in different regions of the plastome. Results: The plastome of P. latifolia was found to be 153,242 base pairs (bp) in length, including a large single copy (LSC) region of 83,603 bp, a small single copy (SSC) region of 18,115 bp, and a pair of inverted repeats (IRs) of 25,762 bp each. It contained 87 protein-coding genes, 8 rRNA genes, and 33 tRNA genes, with an overall GC content of 37.30%. The RSCU analysis revealed regional variation, with the protein-coding region being more conserved than the intergenic spacer. Discussion: This study provides the first complete plastome sequence of Psydrax latifolia, offering insights into its genomic structure and phylogenetic position within the Rubiaceae family. Comparative analyses with 16 Rubiaceae species highlighted distinct genomic features and evolutionary patterns. These findings contribute to the understanding of plastome evolution in the Rubiaceae family and provide a valuable resource for future phylogenetic and evolutionary studies. [ABSTRACT FROM AUTHOR]

A phytochemical investigation on the 80% EtOH extract of the leaves of Paederia scandens (Lour.) Merr. resulted into the isolation of three undescribed iridoid glycosides, 10-O-trans-p-coumaroyl-(4R,6R)-3,4-dihydro-3α-methylthiopaederoside (1), 10-O-trans-feruloyl-(4S,6R)-3,4-dihydro-2′-O-3α-paederoside (2), and 10-O-trans-caffeoyl-paederosidic acid ethyl ester (3). The structures of the new compounds were elucidated by spectral methods such as 1D and 2D (1H-1H COSY, HMQC, and HMBC) NMR spectroscopy, as well as high resolution mass spectrometry. The isolated compounds were tested in vitro for cytotoxic activity against five endocrine tumor cell lines. As a result, compound 1 exhibited some cytotoxicities against all the tested tumor cell lines with IC50 value less than 20.0 μM. [ABSTRACT FROM AUTHOR]

Two uncharacterized monoterpenoids (1 – 2) and six known analogues (3 – 8) were isolated from the fruit of Gardenia jasminoides. Their structures were determined by comprehensive spectroscopic data analysis, and the absolute configuration of 2 was elucidated by comparing the calculated electronic circular dichroism (ECD) spectrum with experimental ECD spectrum. Additionally, the results of preliminary activity evaluation indicated that these compounds showed slightly α -glucosidase inhibitory activity. [Display omitted] • Two undescribed monoterpenoids were isolated from the fruit of Gardenia jasminoides Ellis. • The isolated compounds were evaluated for their α-glucosidase inhibitory activity. [ABSTRACT FROM AUTHOR]

Background: Lasianthus species are widely used in traditional Chinese folk medicine with high medicinal value. However, source materials and herbarium specimens are often misidentified due to morphological characteristics and commonly used DNA barcode fragments are not sufficient for accurately identifying Lasianthus species. To improve the molecular methods for distinguishing among Lasianthus species, we report the complete chloroplast (CP) genomes of Lasianthus attenuatus, Lasianthus henryi, Lasianthus hookeri, Lasianthus sikkimensis, obtained via high-throughput Illumina sequencing. Results: These showed CP genomes size of 160164-160246 bp and a typical quadripartite structure, including a large single-copy region (86675–86848 bp), a small single-copy region (17177–17326 bp), and a pair of inverted repeats (28089–28135 bp). As a whole, the gene order, GC content and IR/SC boundary structure were remarkably similar among of the four Lasianthus CP genomes, the partial gene length and IR, LSC and SSC regions length are still different. The average GC content of the CP genomes was 36.71–36.75%, and a total of 129 genes were detected, including 83 different protein-coding genes, 8 different rRNA genes and 38 different tRNA genes. Furthermore, we compared our 4 complete CP genomes data with publicly available CP genome data from six other Lasianthus species, and we initially screened eleven highly variable region fragments were initially screened. We then evaluated the identification efficiency of eleven highly variable region fragments and 5 regular barcode fragments. Ultimately, we found that the optimal combination fragment' ITS2 + psaI-ycf4' could authenticated the Lasianthus species well. Additionally, the results of genome comparison of Rubiaceae species showed that the coding region is more conservative than the non-coding region, and the ycf1 gene shows the most significant variation. Finally, 49 species of CP genome sequences belonging to 16 genera of the Rubiaceae family were used to construct phylogenetic trees. Conclusions: Our research is the first to analyze the chloroplast genomes of four species of Lasianthus in detail and we ultimately determined that the combination fragment' ITS2 + psaI-ycf4' is the optimal barcode combination for identifying the genus of Lasianthus. Meanwhile, we gathered the available CP genome sequences from the Rubiaceae and used them to construct the most comprehensive phylogenetic tree for the Rubiaceae family. These investigations provide an important reference point for further studies in the species identification, genetic diversity, and phylogenetic analyses of Rubiaceae species. [ABSTRACT FROM AUTHOR]

The classification of Mussaenda L. has been controversial due to the complex interspecific variation and hybridization within the genus. In order to explore the taxonomical significance of leaf venation characteristics between Mussaenda and its relative genera (Schizomussaenda Li and Psudomussaenda Wernham), and to provide data for the identification, development and utilization when they were regarded as the medical and garden plant resources, the leaf venation characteristics of 22 species of this group were observed by using the cleaning method. The cluster analysis was carried out based on the characteristics of leaf venation, and a classification key of the species was compiled. The results were as follows: (1) The characteristics of the leaf venation of Mussaenda and its relative genera had consistency, which were mainly reflected on that the primary veins were all pinnate, the frequency of inter-secondary veins was less than one per areolation, the angles between the major secondary veins and the midveins were acute, the tertiary veins were mostly penetrating, and the arrangement of the areolation were all irregular. (2) The characteristics of the leaf venation with taxonomic value among genera or species within genera included the type of major secondary vein venation type, the angle between major secondary vein and midvein, the major secondary vein spacing, the connection between major secondary vein and midvein, the existence of inter-secondary vein and intramarginal secondary vein, the penetration type of intercostal tertiary vein, the type of quaternary vein, the branching of freely ending veinlet, and the areole development. (3) According to the cluster analysis, 22 species of this group were clustered into 12 branches, and Schizomussaenda and Psudomussaenda were not clustered into one branch respectively, showing a relatively close genetic relationship. In conclusion, the leaf venation characteristics of Mussaenda and its related taxa can provide new information for the classification of the plants. [ABSTRACT FROM AUTHOR]

The species Bathysa gymnocarpa K.Schum is a tree belonging to the Rubiaceae family, endemic in Brazil. So far, there are reports neither of phytochemical work nor of biological evaluation of it. The analysis by High Performance Liquid Chromatography coupled to a Diode Array Detector and a tandem Mass Spectrometer with an Electrospray Ionization source (HPLC‐DAD‐ESI‐MS/MS) of its crude extract allowed to characterize in a complex mixture, without isolation, fourteen compounds, being two as cinnamic acid derivatives, and the others as mono-, di- and triglycosilated derivatives of the flavonols quercetin and kaempferol. These compounds are reported for the first time in Bathysa spp. [ABSTRACT FROM AUTHOR]

Gardenia stenophylla Merr, a member of the genus Gardenia in the family Rubiaceae, possesses significant medicinal and ornamental value and is widely distributed in China. This study reports the newly sequenced chloroplast genome of Gardenia stenophylla Merr. The complete chloroplast genome of Gardenia stenophylla Merr (155,109 bp, GC content of 37.5%) was shown to have a typical quadripartite structure, containing a pair of inverted repeat regions (IRs) of 28,802 bp separated by a large single-copy (LSC) region of 85,396 bp and a small single-copy (SSC) region of 18,109 bp. The chloroplast genome contained 151 genes encoding 106 proteins, 37 tRNAs, and eight rRNAs. The Gardenia stenophylla Merr chloroplast genome displayed the closest phylogenetic relationship to Gardenia jasminoides and Gardenia jasminoides var. grandiflora. These data will assist in future molecular phylogenetics of the Rubiaceae. [ABSTRACT FROM AUTHOR]

Abstract Background Lasianthus species are widely used in traditional Chinese folk medicine with high medicinal value. However, source materials and herbarium specimens are often misidentified due to morphological characteristics and commonly used DNA barcode fragments are not sufficient for accurately identifying Lasianthus species. To improve the molecular methods for distinguishing among Lasianthus species, we report the complete chloroplast (CP) genomes of Lasianthus attenuatus, Lasianthus henryi, Lasianthus hookeri, Lasianthus sikkimensis, obtained via high-throughput Illumina sequencing. Results These showed CP genomes size of 160164-160246 bp and a typical quadripartite structure, including a large single-copy region (86675–86848 bp), a small single-copy region (17177–17326 bp), and a pair of inverted repeats (28089–28135 bp). As a whole, the gene order, GC content and IR/SC boundary structure were remarkably similar among of the four Lasianthus CP genomes, the partial gene length and IR, LSC and SSC regions length are still different. The average GC content of the CP genomes was 36.71–36.75%, and a total of 129 genes were detected, including 83 different protein-coding genes, 8 different rRNA genes and 38 different tRNA genes. Furthermore, we compared our 4 complete CP genomes data with publicly available CP genome data from six other Lasianthus species, and we initially screened eleven highly variable region fragments were initially screened. We then evaluated the identification efficiency of eleven highly variable region fragments and 5 regular barcode fragments. Ultimately, we found that the optimal combination fragment' ITS2 + psaI-ycf4' could authenticated the Lasianthus species well. Additionally, the results of genome comparison of Rubiaceae species showed that the coding region is more conservative than the non-coding region, and the ycf1 gene shows the most significant variation. Finally, 49 species of CP genome sequences belonging to 16 genera of the Rubiaceae family were used to construct phylogenetic trees. Conclusions Our research is the first to analyze the chloroplast genomes of four species of Lasianthus in detail and we ultimately determined that the combination fragment' ITS2 + psaI-ycf4' is the optimal barcode combination for identifying the genus of Lasianthus. Meanwhile, we gathered the available CP genome sequences from the Rubiaceae and used them to construct the most comprehensive phylogenetic tree for the Rubiaceae family. These investigations provide an important reference point for further studies in the species identification, genetic diversity, and phylogenetic analyses of Rubiaceae species.

Nostolachma jenkinsii (Hook.f.) Deb & J.Lahiri, a member of the Rubiaceae family, is an endangered wild plant species with potential economic value. In this research, the complete chloroplast genome of N. jenkinsii was sequenced to gain insight into its genome feature and better understand the phylogenetic relationships among the Rubiaceae species. The chloroplast genome, with a total length of 155,036 bp, comprises two inverted repeats (IR) regions spanning 25,692 bp each, a large single-copy (LSC) region measuring 85,437 bp, and a short single-copy (SSC) region measuring 18,215 bp. There is an overall 37% GC content in the chloroplast genome. By annotation analysis,. 54 tRNA genes, 10 rRNA genes, and 107 protein-coding genes were all annotated in N. jenkinsii. Furthermore, we applied phylogenetic analysis that revealed a close relationship between N. jenkinsii, D. fruticosa and D. dubia, placing them together within the Rubiaceae family.

Psychotria phuquocensis, a new species is described and illustrated from Phu Quoc National Park, Phu Quoc Island in southern Vietnam. It can be distinguished from other Psychotria species previously known in the region by its glabrous stems, triangular, ovate-triangular to ovate stipules, elliptic, lanceolate elliptic or narrowly elliptic oblong leaf blade with small domatia between midrib and basal secondary veins, cymose inflorescence, green flower, and elliptisoid fruits. The color photographs, phenology, distribution, habitat, and preliminary conservation assessment are provided for P. phuquocensis. [ABSTRACT FROM AUTHOR]

Ophiorrhiza paralatiflora, a new species of Rubiaceae from limestone areas of Guangxi, China, is described and illustrated. The new species is morphologically similar to O. alatiflora, but differs from the latter by having an inflorescence that is erect from the youngest developmental stage (versus initially drooping then gradually erect), subulate bract (versus linear or linear‐lanceolate) absent or shorter than 3 mm (versus obvious, 5–15 mm long). It also resembles O. cantonensis, but can be distinguished from the latter by the same characters as from O. alatiflora, plus by having a corolla tube with ca 1 mm (versus 0.3 mm) wide wings outside along its entire length (versus from near apex to the middle part). The conservation status was assessed as 'Least Concern' (LC) according to the IUCN Red List categories and criteria. [ABSTRACT FROM AUTHOR]

Two previously undescribed iridoid glycosides, 6'-O-trans-feruloyl-(4S,6R)-3,4-dihydro-3β-ethoxypaederoside (1) and 6'-O-trans-caffeoyl-(4S,6R)-3,4-dihydro-2'-O-3α-paederoside (2), were isolated from the 90% EtOH extract of the air dried aerial parts of Paederia Foetida. Structural elucidation of all the compounds was performed by spectral methods such as 1D and 2D (1H-1H COSY, HMQC, and HMBC) NMR spectroscopy. The two isolated iridoid glycosides were tested in vivo for their antinociceptive properties. As a result, 2 showed potent antinociceptive effect and its ID50 value (53.4 μmol/kg) was 2-fold less than those of the positive control drugs aspirin and acetaminophen. [ABSTRACT FROM AUTHOR]

Fosbergia shweliensis (Anth.) Tirveng. & Sastre, Fosbergia petelotii Merr. ex Tirveng. & Sastre, and Fosbergia thailandica Tirveng. & Sastre are reported as in extremely small populations in China. The three tree species all belong to the Forbergia of Rubiaceae, and little is known about the structural and evolutionary features of Fosbergia chloroplast genomes. In this study, four chloroplast genomes from three Fosbergia species were assembled, subjected to comparative genomics, and phylogenetically analyzed. The complete chloroplast genomes of Fosbergia showed highly conserved structures and were 154,623–154,730 bp in size, with a GC content of 37.6%. A total of 130 genes were annotated, comprising 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. There are 119–128 long repeats and 39–42 SSRs in four chloroplast genomes. Highly variable loci trnS-trnG and psaA-ycf3 were detected as candidate markers for species identification in Fosbergia. Using 31 complete chloroplast genomes, the phylogenetic relationships among three Fosbergia species were investigated by constructing phylogenetic trees. Fosbergia and Gardenia are sister clades, and F. petelotii is more closely related to F. shweliensis than to F. thailandica. This study provided more molecular data to illuminate the genus Fosbergia evoltution in Rubiaceae. [ABSTRACT FROM AUTHOR]

Nostolachma jenkinsii (Hook.f.) Deb & J.Lahiri, a member of the Rubiaceae family, is an endangered wild plant species with potential economic value. In this research, the complete chloroplast genome of N. jenkinsii was sequenced to gain insight into its genome feature and better understand the phylogenetic relationships among the Rubiaceae species. The chloroplast genome, with a total length of 155,036 bp, comprises two inverted repeats (IR) regions spanning 25,692 bp each, a large single-copy (LSC) region measuring 85,437 bp, and a short single-copy (SSC) region measuring 18,215 bp. There is an overall 37% GC content in the chloroplast genome. By annotation analysis,. 54 tRNA genes, 10 rRNA genes, and 107 protein-coding genes were all annotated in N. jenkinsii. Furthermore, we applied phylogenetic analysis that revealed a close relationship between N. jenkinsii, D. fruticosa and D. dubia, placing them together within the Rubiaceae family. [ABSTRACT FROM AUTHOR]

Background: Uncaria tomentosa (Willd. ex Schult.) DC. (Rubiaceae) is traditionally used by Amazonian indigenous groups to treat inflammatory diseases. To date, there are no systematic reviews and meta-analyses on the use of U. tomentosa for inflammation control in animals supporting the traditional knowledge about this species. This study was conducted to evaluate the effect of U. tomentosa extracts in modulating inflammatory mediators and to determine which types of inflammatory diseases can be treated by this species. Methods: We conducted a systematic review and meta-analysis of preclinical studies published before 26 July 2023, identified in PubMed, Embase, and Scopus. Four independent reviewers extracted the data and assessed the risks of bias. The effects of U. tomentosa on inflammatory diseases and the inflammatory mediators involved were extracted from the studies. Standardized mean differences (SMD) and 95% confidence intervals (95%CI) of the outcomes were estimated. The meta-analyses were conducted using RevMan 5.4 (Cochrane Collaboration). This protocol was registered in PROSPERO (CRD42023450869). Results: Twenty-four of 523 studies were included. U. tomentosa extracts decreased the cytokines interleukin (IL)-6 (SMD: -0.72, 95%CI: -1.15, -0.29, p = 0.001) and transcription factor nuclear factor kappa-B (NF-κB) (SMD: -1.19, 95%CI: -1.89, -0.48, p = 0.001). However, the extracts did not significantly alter IL-1 (SMD: -0.16, 95%CI: -0.87, +0.56, p = 0.67), IL-10 (SMD: -0.05, 95%CI:-0.35, 0.45, p = 0.80), or tumor necrosis factor-alpha (TNF-α) levels (SMD: 0.18, 95%CI: -0.25, 0.62, p = 0.41). Conclusion: Many extracts of stem bark, roots, and leaves of U. tomentosa, mostly aqueous and hydroethanolic, exhibited anti-inflammatory and/or immunomodulatory activities and low toxicity. The extracts decreased NF-κB and IL-6. These findings suggest that this species has the potential to treat inflammatory diseases in which these markers are increased, according to the ethnopharmacological use. These activities are not related to a specific class of compounds. [ABSTRACT FROM AUTHOR]

An unprescribed nortriterpenoid with an aromatic E ring, uncanortriterpenoid A (1), together with fourteen known triterpenoids (2–15), were isolated from the hook-bearing stems of Uncaria rhynchophylla Miq. Based on extensive spectroscopic analyses, the NMR data of 2, 5, and 10 in CD3OD were assigned for the first time, and the wrongly assigned δC of C-27 and C-29 of 2 were revised. Among the known compounds, 7, 13, and 15 were isolated from this species for the first time, and 15 represents the first lanostane triterpenoid bearing an extra methylidene at C-24 for the Rubiaceae family. Additionally, compounds 6 and 14 exhibited moderate ferroptosis inhibitory activity, with an EC50 value of 14.74 ± 0.20 μM for 6 and 23.11 ± 1.31 μM for 14. [ABSTRACT FROM AUTHOR]

The use of molecular data in phylogenetic reconstruction during more than three decades has greatly improved our understanding of the macroevolutionary history of the coffee family (Rubiaceae) and has provided a solid basis for improvement of its classification. Based on the results of 130 studies, among them most recent phylogenomic works, we present a consensus phylogeny and a robust classification of Rubiaceae that shed light on the evolutionary success of this highly diverse angiosperm family and can serve as a framework for ecological and evolutionary studies. There are more than 14,000 species and about 580 accepted genera of Rubiaceae that are assigned to 71 tribes, of which 68 are classified in two subfamilies (Dialypetalanthoideae with 38 tribes and Rubioideae with 30 tribes). Three tribes (Acranthereae, Coptosapelteae, Luculieae) remain unclassified as to subfamily. Sixty‐three of these 71 tribes are assigned to nine informal alliances (four in Rubioideae and five in Dialypetalanthoideae). These tribes are listed in alphabetical order within their respective alliances. Five tribes, one (Coussareeae) in Rubioideae and four (Airospermeae, Jackieae, Retiniphylleae, Steenisieae) in Dialypetalanthoideae, are excluded from these alliances due to unclear or conflicting phylogenetic positions. Thirty‐six tribes retain their tribal status but receive new generic limits to remedy their previous para‐ or polyphyletic nature. Twenty‐nine tribes not implemented in previous classifications have been added, of which three (Chioneae, Glionnetieae, Temnopterygeae) are newly described here. Basic information on phylogenies, distributions, former classifications, and useful references to previous works are provided for all accepted tribes, and future perspectives are discussed. [ABSTRACT FROM AUTHOR]

Phytochemical investigation of Gardenia thunbergia L. f. aerial parts led to the isolation of a previously undescribed neolignan 13, designated (thunbergiside A). This compound is classified as 2,3-dihydrobenzofuran that features a unique, highly substituted skeleton with functional diversity. The absolute configuration was established by comparing its experimental ECD spectrum and optical rotation values with those of related neolignans. In addition, seventeen diverse phytoconstituents were identified for the first time from this plant, expanding our knowledge of its chemical composition. These included oleanane-type saponins, phenolic derivatives, lignans, and neolignans, which serve as valuable chemotaxonomic markers. Considering the frequent emergence of infectious diseases, it is worth exploring additional antimicrobial agents. Selected isolated compounds were assessed for their antimycotic properties in an investigation for potential anti-infective agents. Various chromatographic procedures, spectroscopic, spectrometric analyses, and molecular modelling studies were employed for phytochemical investigation and structural characterization. Furthermore, the alamarBlue-based viability assay was employed for antifungal evaluation against three pathogenic fungi. Specifically, saponin 17 , 3- O -(β-ᴅ-glucuronopyranoside) oleanolic acid, displayed moderate antifungal activity (IC 50 = 11 µg/mL) against Candida albicans. Overall, this study identified an unprecedented neolignan along with other diverse constituents from Gardenia thunbergia. Moreover, the study presented a potential scaffold for antifungal drug development. [Display omitted] • A unique previously undescribed neolignan, thunbergiside A, was isolated. • Valuable chemotaxonomic markers were reported from Gardenia thunbergia. • Antifungal activity of oleanolic acid 3-glucuronide was confirmed. [ABSTRACT FROM AUTHOR]

In this study of evolutionary relationships in the subfamily Rubioideae (Rubiaceae), we take advantage of the off-target proportion of reads generated via previous target capture sequencing projects based on nuclear genomic data to build a plastome phylogeny and investigate cytonuclear discordance. The assembly of off-target reads resulted in a comprehensive plastome dataset and robust inference of phylogenetic relationships, where most intratribal and intertribal relationships are resolved with strong support. While the phylogenetic results were mostly in agreement with previous studies based on plastome data, novel relationships in the plastid perspective were also detected. For example, our analyses of plastome data provide strong support for the SCOUT clade and its sister relationship to the remaining members of the subfamily, which differs from previous results based on plastid data but agrees with recent results based on nuclear genomic data. However, several instances of highly supported cytonuclear discordance were identified across the Rubioideae phylogeny. Coalescent simulation analysis indicates that while ILS could, by itself, explain the majority of the discordant relationships, plastome introgression may be the better explanation in some cases. Our study further indicates that plastomes across the Rubioideae are, with few exceptions, highly conserved and mainly conform to the structure, gene content, and gene order present in the majority of the flowering plants. [ABSTRACT FROM AUTHOR]

Questions: Drought events are increasingly frequent, threatening the biodiversity of tropical forests. The understorey comprises a large fraction of the total plant species richness of these systems with the presence of highly diverse angiosperm families. Here, we quantified the effects of a drought on abundance and functional structures and on the ecosystem functioning of Rubiaceae assemblages along a topographic gradient. Location: Mata das Flores State Park, an Atlantic Forest fragment in southeast Brazil. Methods: Two vegetation surveys were performed: one during an El Niño‐induced drought, and the other three years after this drought. Abundance and functional structures were assessed using the 16 most‐abundant species of Rubiaceae, which comprised 92% of the total abundance. A litter decomposition experiment was carried out to estimate the percentage of mass loss by the Rubiaceae species. We carried out Procrustes analyses on abundance and functional structures and used Generalized Linear Mixed Models to test the effects of drought and topographic habitats on taxonomic and functional compositions, and mass loss. Results: We found that the functional structure and mass loss remained constant despite significant changes in the abundance structure after the drought. Conclusion: The evidence points to the maintenance of ecosystem functioning through functional redundancy, because functionally similar less‐abundant species replaced each other after the drought. We show that swarms of species can maintain biological diversity and stability in ecosystem functioning under drought in the understorey of a tropical forest. [ABSTRACT FROM AUTHOR]

Galium genus belongs to the Rubiaceae family, which consists of approximately 14,000 species. In comparison to its well-known relatives, the plastomes of the Galium genus have not been explored so far. The plastomes of this genus have a typical, quadripartite structure, but differ in gene content, since the infA gene is missing in Galium palustre and Galium trfidum. An evaluation of the effectiveness of using entire chloroplast genome sequences as superbarcodes for accurate plant species identification revealed the high potential of this method for molecular delimitation within the genus and tribe. The trnE-UUC—psbD region showed the biggest number of diagnostides (diagnostic nucleotides) which might be new potential barcodes, not only in Galium, but also in other closely related genera. Relative synonymous codon usage (RSCU) appeared to be connected with the phylogeny of the Rubiaceae family, showing that during evolution, plants started preferring specific codons over others. [ABSTRACT FROM AUTHOR]

Cruckshanksia Hook. & Arn. and Oreopolus Schltdl. Rubiaceae (Rubioideae – Coussareeae), endemic genera of Chile and Andean Argentina, have historically been highly taxonomically unstable. Molecular analyses have confirmed Oreopolus as the sister group of Cruckshanksia; however, the relationships among its species are still not resolved because previous studies have not considered all species of the genus and the published topologies have many unsupported nodes. For this reason, we carried out a phylogenetic study with all the species currently recognized by recent revisions, using two nuclear DNA regions and five plastid regions, to elucidate the relationships between species. In addition, the evolutionary history of the group was estimated based on divergence times, and a character reconstruction was performed. The results corroborate that Oreopolus is the sister group of Cruckshanksia. Cruckshanksia is monophyletic and composed of two principal clades. Clade I is composed of Subclade I (C. pumila and C. verticillata) associated with C. montiana; Clade II is composed of Subclade II (C. palmae and C. macrantha) and Subclade III (C. lithiophila and C. hymenodon). With 81% probability the common ancestor of the genus Cruckshanksia had petaloid appendices associated with two independent lines of evolution. The probability that the common ancestor of the Oreopolus‒Cruckshanksia clade had equal calyx lobes is 50%. [ABSTRACT FROM AUTHOR]

In south Florida, the rough-leaf velvetseed, Guettarda scabra, is limited to fragments of the endangered pine rockland habitat. Its flowers exhibit traits typically associated with attraction of nocturnal hawkmoths (Sphingidae), although they are abundantly visited by diurnal lepidopterans. These observations question whether the sphingophilous nature of this species represents actual dependence on nocturnal visitors for sexual reproduction. A previous study found diurnal pollination in G. scabra; however, the results may have been skewed by extensive plant manipulations. To address this, we conducted a more comprehensive investigation to re-examine the role of diurnal visitors in the plant's reproduction. We evaluated the relative contribution of diurnal and nocturnal flower visitors to female reproductive success of G. scabra in its natural habitat. We selectively exposed flowers to these visitor groups and identified all the lepidopteran visitors observed. We found that plants exposed exclusively to nocturnal visitors exhibited significantly higher fruit-set than those only exposed to diurnal visitors, whose fruit-set was minimal and equivalent to that of plants never exposed to visitors. These findings indicate that nocturnal visitors are essential for successful sexual reproduction of G. scabra. Our field observations revealed that at night, flowers of G. scabra are visited by the hawkmoth Xylophanes tersa, suggesting a specialized pollination system in which hawkmoths are effective pollen vectors of this plant. Contrary to earlier conclusions, we demonstrate that flowers of G. scabra are exclusively pollinated by nocturnal moths, despite more frequent legitimate visitation by diurnal lepidopterans, which therefore act as nectar and pollen thieves. [ABSTRACT FROM AUTHOR]

Copyright of Ecosistemas y Recursos Agropecuarios is the property of Universidad Juarez Autonoma de Tabasco and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The characterization of colleters in Rubiaceae is crucial for understanding their role in plant function. Analyzing colleters in Palicourea tetraphylla and Palicourea rudgeoides aims to deepen the understanding of these structures morphoanatomical and functional characteristics. The study reveals colleters with palisade epidermis and a parenchymatic central axis, classified as standard type, featuring vascularization and crystals. Colleter secretion, abundant in acidic mucopolysaccharides, proteins, and phenolic compounds, protects against desiccation. The ontogenesis, development, and senescence of the colleters are quite rapid and fulfill their role well in biotic and abiotic protection because these structures are present at different stages of development in the same stipule. Pronounced protrusions on the colleters surface, coupled with the accumulation of secretion in the intercellular and subcuticular spaces, suggest that the secretory process occurs through the wall, driven by pressure resulting from the accumulation of secretion. The microorganisms in the colleters' secretion, especially in microbiota-rich environments such as the Atlantic Forest, provide valuable information about plant–microorganism interactions, such as resistance to other pathogens and organisms and ecological balance. This enhanced understanding of colleters contributes to the role of these structures in the plant and enriches knowledge about biological interactions within specific ecosystems and the family taxonomy. [ABSTRACT FROM AUTHOR]

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ABSTRACT: The genipap (Genipa americana L.) is a non-endemic species native to Brazil belonging to the family Rubiaceae. It is a species that unites socioeconomic and environmental pillars. The study objective was to establish the induction of indirect somatic embryogenesis in foliar explants of genipap genotypes. Leaf explants of UMB, SAL, JSA, SC, and CER accessions cultivated in half the salt concentration of Murashige & Skoog (MS), 30 g/L sucrose, and 3g/L PhytagelTM with the following combinations of NAA × BA regulators were used: M1: 4.0/4.0 mg/L NAA and BA, M2: 4.0/6.0 mg/L NAA and BA, and M3: 6.0/4.0 mg/L NAA and BA. At 60 days of culture, they were transferred to secondary embryogenic callus multiplication medium supplemented with 2.21 mg/L of 2,4-D. At 30 and 60 days, the increment (%) and fresh mass (g) of primary callus and at 120 days the presence of embryogenic callus were evaluated. The primary medium with 4.0 mg/L of NAA and 6.0 mg/L of BA induced embryogenic primary callus in leaf explants of the genipap accessions SAL, SC, and JSA. The secondary medium was promising for the multiplication of embryogenic callus. Cytochemical analysis confirmed the presence of embryogenic cells in SAL, SC, and JSA accessions.

Nauclea pobeguinii is a plant in the Rubiaceae family endemic to the swamp forest region of the world. Its extract is widely used in traditional medicine for the treatment of a wide variety of ailments such as malarial, Jaundice, gonorrhea, fever, and stomach discomfort. While other parts of the plant have been examined for the bioactive principles responsible for the medicinal properties, limited information is available in the literature as regards the leaves, hence this study. N. pobeguinii leaves were collected, air-dried, and pulverized. The pulverized sample was extracted with solvents (n-hexane, ethyl acetate, and ethanol) of varying polarity to obtain the crude extracts. Repeated column and thin layer chromatographic separation of the crude extracts afforded three compounds, which were characterized by their IR, 1H, 13C-NMR, and 2D-NMR spectral data. A comparison of the data with literature confirmed the compounds to be 2-hydroxylstigmastane acetate (1), Ergosta-5,6-epoxy-22-en-3-yl-acetate (2), and β-sitosterol (3). Compounds 1 and 2 are novel to N. pobeguinii.

Two new species of eriophyoid mites, Shevtchenkella holoptele sp. nov. from Holoptelea integrifolia (Roxb.) (Ulmaceae) and Diptilomiopus cordifoliae sp. nov. from Haldina cordifolia (Roxb.) (Rubiaceae) were described and illustrated from New Alluvial Zone of West Bengal, India. The two new mite species were found to be vagrant on under surface of the leaves without causing visible damage symptoms. [ABSTRACT FROM AUTHOR]

Two new bisanthraquinones, glabraquinone A and B (1–2) were isolated from the root of Prismatomeris glabra (Korth.) Valeton. In addition to the new glabraquinones, six known anthraquinones, that is, 1-hydroxy-2-methoxy-6-methylanthraquinone (3), 1,2-dimethoxy-7-methylanthraquinone (4), lucidin (5), nordamnacanthal (6), damnacanthal (7) and 2-carboxaldehyde-3-hydroxyanthraquinone (8)) and an aromatic compound, that is, catechol diethyl ether (9) were isolated and characterized in this study. Compounds 1, 4 and 9 showed mild activity, reducing N2A cell viability to 77%, 82% and 77%, respectively, in anti-neuroblastoma assay. [ABSTRACT FROM AUTHOR]

The Rubiaceae family, comprising 550 species in the Philippines, is a significant source of bioactive components with ethnopharmacological uses. This study assessed the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity of six Rubiaceae species, collected in Ilocos Norte, Philippines: Guettardella microphylla (Bartl. ex DC.) Merr., Timonius ternifolius (Bartl. ex DC.) Fern.-Vill., Kanapia monstrosa (A. Rich.) Arriola & Alejandro, Pyrostria triflora Arriola, Calaramo & Alejandro, Pyrostria subsessilifolia (Merr.) Arriola & Alejandro, and Psychotria luzoniensis (Cham. & Schltdl.) Fern.-Vill. Identification of the plant species was done using morphological characterization. The TFC results ranged between 1.86-3.81 mg quercetin equivalent/g dry weight (GAE/g DW), while TPC indicated 5.47-17.17 mg gallic acid equivalent/g dry weight (QE/g DW). G. microphylla showed the highest TFC (3.81±0.20 mg QE/g DW) while P. triflora exhibited the highest TPC (17.17±0.83 mg GAE/g DW). Antioxidant profiling showed P. luzoniensis exhibiting the highest activity in the NOS, ABTS, DPPH, and FRAP assays. G. microphylla showed the highest hydrogen peroxide scavenging activity, while T. ternifolius demonstrated the highest hydroxyl radical scavenging activity. Findings suggest that the crude methanolic extracts of the Rubiaceae species have relatively high TPC and TFC values and exhibit promising antioxidant capacities. [ABSTRACT FROM AUTHOR]

Synthesis of the Alibertia edulis (Rubiaceae) interaction with the ant Camponotus rufipes. The nectary in the flowers and later in the apex of the fruit (pericalpial nectary) offers resources for the ants during the reproductive season of Alibertia. These ants are recognized for holding an aggressive behavior that might protect the plant from herbivores and fruit predators. When the fruit is ripe, the interaction ends until the next reproduction season for Alibertia edulis. [Display omitted] • Considering the phenological, anatomical and histochemical tests, we describe a new association between the plant Alibertia edulis and the ant Camponotus rufipes, both widely spread Cerrado species. • The plant structure involved in the interaction is a pericarpial nectary. • The interaction occurs during the fruit ripening phase, in which pericarpial nectaries in unripe fruits are secreting sugar. When the fruit is completely ripe, in the peak of the rainy season, the interaction ends until the next reproduction season for Alibertia. Plant-ant interaction in savanna ecosystems is an iconic example of species mutualism. In Cerrado, Brazilian savanna, it is common to find this type of interaction mediated by the offer of extrafloral rewards. This study described a new plant-ant interaction for the tree Alibertia edulis (Rubiaceae) and the ant species Camponotus rufipes and elucidated the main structure involved in the interaction. To do so, (i) we monitored the phenology of A. edulis , (ii) recorded the ant species visitation, and, (iii) collected fruits in different stages of development to perform anatomical and histochemical trials to characterize the structures and chemical components related to the secreted substances - which could be responsible for attracting the ants. Ants' visitation starts at the beginning of fruit development and stops when they are completely ripe. An extrafloral nectary – pericarpical nectary - on the top of the fruits was identified and described for the first time in the Alibertia edulis , derived from the flower structure, which remains to exude (sugar components) during the fruit development. Based on these results, C. rufipes , known for being an aggressive ant, may play a role in protecting fruits against predation until they are completely ripe and the seeds are ready to be dispersed. During seed dispersal, the fruits reduce the production of nectar, signaling the end of the partnership with the ants until the next reproductive season. Here, we shed light and bring new insights into a new plant-ant interaction for a widely spread tree and ant species. [ABSTRACT FROM AUTHOR]

The new species, Thliphthisa sapphus sp. nov. (Rubiaceae, Rubieae), a narrow endemic of the white cliffs of Lefkátas on the southwest coast of Lefkada (Greece) is described and illustrated and an IUCN assessment is presented. Vegetation relevés were performed at the single known locality, limestone cliffs facing the sea and revealed a new association, the Thliphthisa sapphus-Lomelosietum dallaportae. The chromosome number of Thliphthisa sapphus was determined as 2n = 4x = 44, being the single tetraploid species in the genus to date. The species also differs markedly morphologically from its morphologically closest relatives, two Greek steno-endemic oreophytes, Th. baenitzii and Th. muscosa by the following characters: densely setose mericarps and corolla, tetraploidy and by its distribution. An identification key for the Greek species of Thliphthisa is provided. Th. sapphus constitutes the westernmost outpost of a group of Greek steno-endemics, highlighting the importance of coastal habitats and their protection as refugia for poorly competitive chamaephytes. [ABSTRACT FROM AUTHOR]

Promecops leucothyrea Fahraeus (Coleoptera: Curculionidae), is reported for the first time in Chiapas. Adults feed on soybean plants from VC stage (unrolled unifoliolate leaves) to R2 (full flowering). Although P. leucothyrea is currently found only in a few soybean fields in Chiapas, to our knowledge, it could spread to other parts of Mexico. Other plants that were found as hosts for P. leucothyrea were: Rottboellia cochinchinensis (Lour.) W. Clayton, Sorghum halepense (L.) Persoon (Poaceae), Ipomoea trifida (Kunth) G. Don (Convolvulaceae), Azadirachta indica A. Juss. (Meliaceae), Hamelia rovirosae Wernham. (Rubiaceae), Malvastrum coromandelianum (L.) Garcke (Malvaceae). Se reporta por primera vez a Promecops leucothyrea Fahraeus (Coleoptera: Curculionidae), en Chiapas. Los adultos se alimentan en plantas de soya en la etapa VC (hojas unifoliadas desenrolladas) hasta R2 (plena floración). Aunque P. leucothyrea, se encuentra en este momento solo en ciertas localidades con cultivos de soya en Chiapas, a nuestro conocimiento, probablemente pueda esparcirse a otras partes de México. Otras plantas hospederas de P. leucothyrea que se encontraron en Chiapas son Rottboellia cochinchinensis (Lour.) W. Clayton, Sorghum halepense (L.) Persoon (Poaceae), Ipomoea trifida (Kunth) G. Don (Convolvulaceae), Azadirachta indica A. Juss. (Meliaceae), Hamelia rovirosae Wernham. (Rubiaceae), Malvastrum coromandelianum (L.) Garcke (Malvaceae). [ABSTRACT FROM AUTHOR]

Continued extensive field exploration in Zamboanga Peninsula (Philippines) revealed a new endemic Ophiorrhiza species, which is herein described from Naga‐Kabasalan Protected Landscape (NKPL) of Zamboanga Sibugay. The species is unique among Philippine Ophiorrhiza by having leaf lamina with gray to silver gray abaxial surface. The new species is morphologically similar to O. pubescens and O. erythropilosa by having hairy overall morphology, but it is easily distinguished by having a creeping to decumbent habit and inconspicuous, membranous bracts. A detailed description, color photographs, phenology, geographical distribution, habitat and IUCN conservation status are provided in this paper. [ABSTRACT FROM AUTHOR]

A study on floristic composition, community structure and biomass of Rubiaceae was carried out in Bangi Permanent Forest Reserve, Selangor. In the 0.45 ha of ecological plots, a total of 17 species from three genera of Rubiaceae was recorded for trees with a diameter = 1.0 cm DBH. Three genera viz. Canthium, Ixora and Urophyllum were represented by two species each, while remaining genera comprised of a single species each. Shrub group represented the largest total number of species with seven species followed by the understorey with four species. The range of tree DBH in the study plot was between 1.0 and 31.3 cm. The genus with highest density was Aidia, viz. 244 trees/ha, represented by Aidia densiflora. Total basal area for Rubiaceae was 1.69 m2/ha, with the genus Aidia, specifically the species Aidia densiflora, contributing the highest basal area of 0.57 m2/ha (33.91%). Total biomass estimate (above ground and below ground) for this family was 16.71 t/ha. At genus level, Timonius contributed the highest total biomass value, viz. 5.82 t/ha (34.85%), represented by the species Timonius wallichianus. [ABSTRACT FROM AUTHOR]

A new naphthoquinone derivative (1) together with twenty-three known compounds (2–24), were isolated from the aerial parts of Rubia cordifolia L. Their structures were elucidated on the basis of NMR and HR-ESIMS data. Compounds 1–13 were assessed for their inhibitory effects on NO production in LPS-stimulated RAW 264.7 macrophage cells. Compounds 2-6 exhibited significant inhibitory activities with IC50 values of 21.37, 13.81, 24.56, 20.32, and 30.08 μmol·L−1, respectively. [ABSTRACT FROM AUTHOR]

The paper provides an identification key and an annotated list of all six species of the genus Ridsdalea J.T.Pereira & K.M.Wong (Rubiaceae, Gardenieae) recorded in the flora of Vietnam, along with data on their valid names, synonyms, types, and distribution. A new species, R. backanensis, discovered in the limestone area of Bac Kan Province (northern Vietnam), is described and illustrated. Detailed data on its characters, ecology, distribution, phenology, preliminary IUCN conservation status, and taxonomical notes are given. The newly discovered species is potentially interesting for cultivation as an ornamental tree that may be effectively used in garden and urban green architecture. [ABSTRACT FROM AUTHOR]

Mutualisms have driven the evolution of extraordinary structures and behavioural traits, but their impact on traits beyond those directly involved in the interaction remains unclear. We addressed this gap using a highly evolutionarily replicated system – epiphytes in the Rubiaceae forming symbioses with ants. We employed models that allow us to test the influence of discrete mutualistic traits on continuous non‐mutualistic traits. Our findings are consistent with mutualism shaping the pace of morphological evolution, strength of selection and long‐term mean of non‐mutualistic traits in function of mutualistic dependency. While specialised and obligate mutualisms are associated with slower trait change, less intimate, facultative and generalist mutualistic interactions – which are the most common – have a greater impact on non‐mutualistic trait evolution. These results challenge the prevailing notion that mutualisms solely affect the evolution of interaction‐related traits via stabilizing selection and instead demonstrate a broader role for mutualisms in shaping trait evolution. [ABSTRACT FROM AUTHOR]

Ophiorrhiza reflexa, a new species from Guangxi, China, is described and illustrated in this study. It is morphologically similar to O. alatiflora due to the branched inflorescence, distylous flowers and the tubular-funnelform corolla with five longitudinal wings. The new species can be distinguished from O. alatiflora by its erect inflorescence, its smaller and equal-sized calyx lobes 0.5–0.7 mm long, its corolla tubes winged to the middle and the wings straight and its strongly reflexed corolla lobes at anthesis. Ophiorrhiza reflexa is assessed as least concern (LC) according to IUCN Categories and Criteria. [ABSTRACT FROM AUTHOR]

Background: Most Rubiaceae species are hermaphroditic with outcrossing often promoted by distyly and self-incompatibility, as well as by andromonoecy and dioecy in some species. However, cleistogamy has been reported in three genera, including Richardia. Aims: To characterise the reproductive biology of Richardia brasiliensis (Rubiaceae), a ruderal herb native to southern USA, Central America and large parts of South America, and determine if it exhibits preanthesis cleistogamy. Methods: In three natural populations of R. brasiliensis in Argentina, floral processes were monitored in the flower-bud stage and during anthesis until senescence. In addition, stigma receptivity and pollen viability were determined at these different floral stages in two populations, and experimental controlled pollinations were performed to check for spontaneous self-pollination, natural pollination and apomixis. Floral visiting insects were observed and collected for identification. Results: Autonomous self-pollination occurred when flowers were in bud and stigmas were receptive, with ovules fertilised prior to anthesis. These flowers were protogynous and functionally cleistogamous, though later passed through a chasmogamous phase when flowers opened and were visited by diverse insects. Conclusions: Our study demonstrates that preanthesis cleistogamy occurs in flower-buds of R. brasiliensis, indicating that this mode of self-pollination and self-fertilisation may be more common in Rubiaceae than previously realised. KEY HIGHLIGHTS: Flowers of Richardia brasiliensis exhibit two striking features in the flower-bud stage: stigmatic receptivity and self-pollination causing preanthesis cleistogamy to occur. Pollen grain germination on distinctly enlarged papillae cells and pollen tube growth in the style to the ovary were observed in 85% of fixed flower-buds. During anthesis, flowers are visited by a diversity of insects collecting pollen and nectar (Orders Hymenoptera (Apidae, Formicidae and Trigonalidae) and Diptera (Syrphidae)), but due to preanthesis cleistogamy most reproduction occurs via self-fertilisation. [ABSTRACT FROM AUTHOR]

Context: Seed banking is the most efficient and cost-effective method of preserving plant germplasm, but not all species can be conserved by conventional practices. Further, restoration of plant populations from seeds often is hindered by a lack of a priori knowledge of seed dormancy and germination requirements. Aims: Our objective was to determine seed desiccation, initial freezing tolerance and the dormancy-breaking and germination requirements of Coprosma kauensis , a dioecious shrub endemic to Kauaʻi Island in Hawaiʻi. Methods: The effects of temperature and gibberellic acid (GA3) on germination of fresh seeds were tested in light/dark at 15/5, 25/15 and 20/10°C with and without treatment with GA3. To test effects of desiccation and initial freezing on germination, seeds were dried to 15% relative humidity at 15°C and then stored at −20°C. Key results: Regardless of incubation temperature and treatment (or not) with GA3, final germination ranged from 78 to 88%; however, time to 50% germination decreased at 20/10°C for seeds treated with 500 ppm GA3. There was no significant difference in final germination percentage between freshly harvested seeds and those desiccated, nor of those desiccated and then subsequently frozen at −20°C. Conclusions: Fresh seeds of C. kauensis exhibit a low degree of nondeep physiological dormancy. They can germinate over a range of temperatures, but germination speed is increased by GA3. Fresh seeds are desiccation and freeze tolerant (after 6 months storage). Implications: This vulnerable Kauaʻi endemic species easily can be propagated from seeds. Future studies should investigate long-term longevity at various storage temperatures. Seeds of Coprosma kauensis , an IUCN Red List Vulnerable endemic Hawaiian species, germinate over a range of temperatures, but germination rate (speed) is increased by gibberellic acid (GA3). Fresh seeds are desiccation tolerant and can survive short-term storage at −18°C. However, future studies are needed to determine the optimal temperature for long-term storage. [ABSTRACT FROM AUTHOR]

The genus Cinchona L. has important medicinal, cultural, and economic value and is the emblematic tree of Peru. The genus is mainly found in the cloud forests of the Andes. However, the expansion of agriculture and livestock farming in the department of Amazonas is degrading these ecosystems and has reduced the size of the genus's populations. In this work, we model the potential distribution under current conditions of three Cinchona species (C. capuli L. Anderson, C. macrocalyx Pav. Ex DC., and C. pubescens Vahl.) to identify areas with a high likelihood of species presence and their key conservation and reforestation zones. We fitted a maximum entropy (MaxEnt) model using nineteen bioclimatic variables, three topographic variables, nine edaphic variables, and solar radiation. Under current conditions, the potential distribution of C. capuli covers 17.22% (7243.98 km2); C. macrocalyx, 29.11% (12,238.91 km2); and C. pubescens, 22.94% (9647.63 km2) of the study area, which was mostly located in central and southern Amazonas. Only 24.29% (25.51% of C. capuli, 21.02% of C. macrocalyx, and 26.35% of C. pubescens) of the potential distributions are within protected areas, while 10,987.22 km2 of the surface area of the department of Amazonas is degraded, of which 29.80% covers the area of probable occurrence of C. capuli, 38.72% of C. macrocalyx, and 34.82% of C. pubescens. Consequently, it is necessary to promote additional conservation strategies for Cinchona, including the establishment of new protected areas and the recovery of degraded habitats, in order to protect this species. [ABSTRACT FROM AUTHOR]

A polinização é um serviço ecossistêmico de grande importância para a conservação da biodiversidade. A maior parte dos alimentos consumidos por nós depende em algum grau ou totalmente da polinização realizada por abelhas. Embora o café seja autocompatível, estudos apontam que em cultivo sombreado e próximo a áreas florestadas, as flores recebem um maior número de visitantes, o que contribui para uma produção maior. O presente estudo teve como objetivo comparar a diversidade de insetos polinizadores, taxa de visitação e produção de café a pleno sol e sombreado na região do sul de Minas. As observações florais aconteceram nos meses de setembro e outubro de 2017, e setembro de 2018. As abelhas do gênero Trigona foram os visitantes florais mais abundantes no café com 116 visitas e Plebeia sp. foi a abelha mais frequente nas flores de café (70,3%). Os principais visitantes florais foram abelhas nativas. As áreas de cultivo mais sombreadas foram as que mais receberam visitas nas flores de café, e o talhão com a terceira maior porcentagem de sombreamento (17,53%), apresentou a maior média de tamanho das sementes.

Este es el cuarto artículo de una serie dedicada a la flora de Los Farallones de Cali. El propósito general de la serie es hacer un inventario de las plantas, algas y hongos estudiando localidades puntuales en Los Farallones. Este artículo está dedicado a las especies del orden Gentianales, familias Asclepiadaceae y Rubiaceae del bosque de Pico de Águila, en el departamento del Valle del Cauca, Colombia. Para hacer este inventario se recolectaron especímenes que fueron secados en el herbario CUVC y enviados a especialistas para su identificación. Se presentan datos sobre la distribución altitudinal y biogeografica de las especies para Colombia por departamentos y algunas ilustraciones para facilitar su identificación. Varias de las especies halladas tienen potencial para estudios de bioprospección.

The Rubiaceae family, comprising 550 species in the Philippines, is a significant source of bioactive components with ethnopharmacological uses. This study assessed the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity of six Rubiaceae species, collected in Ilocos Norte, Philippines: Guettardella microphylla (Bartl. ex DC.) Merr., Timonius ternifolius (Bartl. ex DC.) Fern.-Vill., Kanapia monstrosa (A. Rich.) Arriola & Alejandro, Pyrostria triflora Arriola, Calaramo & Alejandro, Pyrostria subsessilifolia (Merr.) Arriola & Alejandro, and Psychotria luzoniensis (Cham. & Schltdl.) Fern.-Vill. Identification of the plant species was done using morphological characterization. The TFC results ranged between 1.86-3.81 mg quercetin equivalent/g dry weight (GAE/g DW), while TPC indicated 5.47-17.17 mg gallic acid equivalent/g dry weight (QE/g DW). G. microphylla showed the highest TFC (3.81±0.20 mg QE/g DW) while P. triflora exhibited the highest TPC (17.17±0.83 mg GAE/g DW). Antioxidant profiling showed P. luzoniensis exhibiting the highest activity in the NOS, ABTS, DPPH, and FRAP assays. G. microphylla showed the highest hydrogen peroxide scavenging activity, while T. ternifolius demonstrated the highest hydroxyl radical scavenging activity. Findings suggest that the crude methanolic extracts of the Rubiaceae species have relatively high TPC and TFC values and exhibit promising antioxidant capacities.

BackgroundUncaria tomentosa (Willd. ex Schult.) DC. (Rubiaceae) is traditionally used by Amazonian indigenous groups to treat inflammatory diseases. To date, there are no systematic reviews and meta-analyses on the use of U. tomentosa for inflammation control in animals supporting the traditional knowledge about this species. This study was conducted to evaluate the effect of U. tomentosa extracts in modulating inflammatory mediators and to determine which types of inflammatory diseases can be treated by this species.MethodsWe conducted a systematic review and meta-analysis of preclinical studies published before 26 July 2023, identified in PubMed, Embase, and Scopus. Four independent reviewers extracted the data and assessed the risks of bias. The effects of U. tomentosa on inflammatory diseases and the inflammatory mediators involved were extracted from the studies. Standardized mean differences (SMD) and 95% confidence intervals (95%CI) of the outcomes were estimated. The meta-analyses were conducted using RevMan 5.4 (Cochrane Collaboration). This protocol was registered in PROSPERO (CRD42023450869).ResultsTwenty-four of 523 studies were included. U. tomentosa extracts decreased the cytokines interleukin (IL)-6 (SMD: −0.72, 95%CI: −1.15, −0.29, p = 0.001) and transcription factor nuclear factor kappa-B (NF-κB) (SMD: −1.19, 95%CI: −1.89, −0.48, p = 0.001). However, the extracts did not significantly alter IL-1 (SMD: −0.16, 95%CI: −0.87, +0.56, p = 0.67), IL-10 (SMD: −0.05, 95%CI:–0.35, 0.45, p = 0.80), or tumor necrosis factor-alpha (TNF-α) levels (SMD: 0.18, 95%CI: −0.25, 0.62, p = 0.41).ConclusionMany extracts of stem bark, roots, and leaves of U. tomentosa, mostly aqueous and hydroethanolic, exhibited anti-inflammatory and/or immunomodulatory activities and low toxicity. The extracts decreased NF-κB and IL-6. These findings suggest that this species has the potential to treat inflammatory diseases in which these markers are increased, according to the ethnopharmacological use. These activities are not related to a specific class of compounds.Systematic Review Registration:https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=450869, Identifier CRD42023450869.