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9 results on '"RONGÉ-COLLARD, E."'

3. Lactoferrin: no evidence for its role in regulation of CSA production by human lymphocytes and monocytes

Author

Stryckmans, P, Delforge, A, Amson, R, Prieels, J, Telerman, Adam, Bieva, C, Deschuyteneer, M, Rongé-Collard, E, Université libre de Bruxelles (ULB), and Telerman, Adam

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [SDV]Life Sciences [q-bio], Bone Marrow Cells, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Lactoglobulins, Monocytes, [SDV] Life Sciences [q-bio], Colony-Forming Units Assay, Lactoferrin, Microscopy, Electron, Colony-Stimulating Factors, Liver, Species Specificity, Bone Marrow, Animals, Humans, Cattle, Lymphocytes, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Cell Division, Cells, Cultured
Abstract

International audience; Lactoferrin (LF) has been recently proposed as a physiologic regulator of the granulocyte monocyte progenitor (CFU-GM). This glycoprotein, when saturated with iron, has been said to limit CFU-GM growth by decreasing production and release of colony stimulating activity (CSA) by monocytes and macrophages. Human milk LF saturated with iron, at concentrations ranging from 10(-18) to 10(-8) M was added either to endogenously stimulated bone marrow cells or to mononucleated cells used as feeder layers for adherent cell-depleted marrow. Irrespective of the concentration of LF within the culture system used, no significant inhibition of CFU-GM growth was observed. Moreover, the CFU-GM stimulating activity of medium conditioned by a 4-day incubation of 1 X 10(6) mononucleated blood cells in the presence or in the absence of LF was the same. Various possible explanations for not confirming the reported inhibiting activity of iron saturated LF were explored: 1) masking inhibition of the system by prostaglandin E2 (PGE2), 2) masking inhibition of the system by bovine LF still detectable in the fetal calf serum after heating, 3) preinhibition of the system by leukemic-associated inhibitory activity (LIA) possibly present in the culture system, 4) the iron and calcium content of the culture medium used, 5) the fixation of LF to plastic compounds, 6) the source of the human LF used, 7) the marrow cell separation methods used. None of these factors was shown to play a role in vitro in the activity of LF and thus no evidence was found for a significant role of LF in the regulation of CSA production by monocytes. Peripheral blood human monocytes isolated by elutriation and incubated in albumin free medium in the presence of either 125I-LF or colloidal gold-labeled LF showed no LF binding.

Published
1984

7. Effect of 4-hydroperoxycyclophosphamide on leukemic and normal human myeloid progenitor cells.

Author

Delforge A, Loos M, Stryckmans P, Socquet M, Debusscher L, and Rongé-Collard E

Subjects
Acute Disease, Bone Marrow pathology, Cyclophosphamide administration & dosage, Cyclophosphamide pharmacology, Cyclophosphamide therapeutic use, Dose-Response Relationship, Drug, Hematopoietic Stem Cells drug effects, Humans, Leukemia drug therapy, Leukemia, Myeloid, Acute drug therapy, Leukemia, Myeloid, Acute pathology, Tumor Stem Cell Assay, Cyclophosphamide analogs & derivatives, Granulocytes pathology, Hematopoietic Stem Cells pathology, Leukemia pathology
Abstract

The sensitivity of myeloid progenitor cells from normal subjects (N-CFU-GM) and from leukemic patients in complete remission (LR-CFU-GM) to 4-hydroperoxycyclophosphamide (4-HC) were compared to the sensitivity of leukemic progenitor cells (L-CFU) to this drug. The results were expressed as the dose of 4-HC needed to kill 90% (TD 90) of the progenitor cells. The mean TD 90 were respectively for N-CFU-GM : 59 (+/- 11 S.E.M.) nM ml-1 and for L-CFU 79 (+/- 6 S.E.M.) nM ml-1. Thus, L-CFU were equally sensitive to 4-HC as N-CFU-GM. Moreover, the mean TD 90 for LR-CFU-GM was 87 (+/- 5 S.E.M.) nM ml-1. Thus, the sensitivity of N-CFU-GM and LR-CFU-GM did not differ significantly from that of L-CFU. These results are not encouraging for the use of 4-HC in vitro to eliminate the residual leukemic cells from autologous bone marrow of AML patients in complete remission. The sensitivity of L-CFU was modified neither by previous cytoreductive therapy (different from cyclophosphamide) nor by the time elapsed since diagnosis of AML.

Published
1985
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8. Increased levels of myeloid progenitor cells in the blood of patients with metastatic invasion of the marrow.

Author

Delforge A, De Caluwe JP, Rongé-Collard E, Mattelaer MA, Spiro T, Van den Heule B, and Stryckmans PA

Subjects
Colony-Forming Units Assay, Hematopoietic Stem Cells cytology, Humans, Neoplastic Cells, Circulating, Bone Marrow pathology, Breast Neoplasms blood, Lung Neoplasms blood
Abstract

Granulocyte-macrophage clusters and colony-forming cells (CFU-C) in the peripheral blood have been studied in 26 cancer patients with neoplastic bone marrow involvement. The concentration of CFU-C in the blood of normal individuals and of cancer patients with no bone marrow invasion, ranged from 0 to 99 ml. In contrast, out of 27 cancer patients with marrow invasion, 9 (35%) showed a significant increase of blood CFU-C (100 to 21000/ml) and of those 5 (19%) showed an increase of blood colonies (41 to 9000/ml). There was a strong correlation between increased CFU-C or colony concentration and the presence of myeloid or/and erythroid immature cells in the peripheral blood. On the other hand, there was no apparent correlation between an increased CFU-C level and anaemia or abnormal blood leucocyte count or marrow fibrosis. These observations suggest that bone marrow involvement by neoplastic cells may cause spatial redistribution of the granulocyte macrophage progenitor cells.

Published
1983
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