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This paper examines business model implementations in three leading European smart cities: London, Amsterdam, and Berlin. Through a systematic literature review and comparative analysis, the study identifies and analyzes various business models employed in these urban contexts. The findings reveal a diverse array of models, including public–private partnerships, build–operate–transfer arrangements, performance-based contracts, community-centric models, innovation hubs, revenue-sharing models, outcome-based financing, and asset monetization strategies. Each city leverages a unique combination of these models to address its specific urban challenges and priorities. The study highlights the role of PPPs in large-scale infrastructure projects, BOT arrangements in transportation solutions, and performance-based contracts in driving efficiency and accountability. It also explores the benefits of community-centric models, innovation hubs, revenue-sharing models, outcome-based financing, and asset monetization strategies in enhancing the sustainability, efficiency, and livability of smart cities. The paper offers valuable insights for policymakers, urban planners, and researchers seeking to advance smart city development worldwide.

Abstract The aim of this study was to evaluate the changes in the serum and salivary inflammatory markers induced by Diabetes mellitus (DM) in dogs and to assess the possible confounding effect of gingivitis. A panel of 13 cytokines was measured in the serum and saliva of dogs diagnosed with DM and compared with healthy dogs without gingivitis (control group 1; CG1) and dogs with gingivitis but otherwise healthy (control group 2; CG2). The results of the present study showed statistically significantly higher levels of IL-8, KC-like and MCP1 in the serum of dogs with DM compared to CG1 dogs. In the case of saliva, the DM group presented statistically higher GM-CSF, IL6, IL15, and MCP1 levels compared to CG1, and lower KC-like chemokine compared to CG2. Finally, gingivitis produced changes in saliva, with salivary levels of GM-CSF, IL-6, IL-7, IL-15, IP-10, KC-like, IL-10, IL-18, MCP1, TNFα being statistically significantly higher in the saliva of CG2 dogs compared to CG1. The results of the present study indicate that dogs with DM have altered cytokine levels in serum and saliva compared to healthy dogs. In addition, this study highlights the importance of taking oral health into account when determining cytokines in dogs, as gingivitis can significantly alter their concentrations. .

Studying the influence of the host plant genotype on the spectral reflectance of crops infected by a pathogen is one of the key directions in the development of precision methods for monitoring the phytosanitary state of wheat agrocenoses. The purpose of this research was to study the influence of varietal factors and disease development on the spectral characteristics of winter wheat varieties of different susceptibility to diseases during the growing seasons of 2021, 2022 and 2023. The studied winter wheat crops were represented by three varieties differing in susceptibility to phytopathogens: Grom, Svarog and Bezostaya 100. Over three years of research, a clear and pronounced influence of the varietal factor on the spectral characteristics of winter wheat crops was observed, which in most cases manifested itself as an immunological reaction of specific varieties to the influence of pathogen development. The nature of the influence of the pathogenic background and the spectral characteristics of winter wheat crops were determined by the complex interaction of the development of individual diseases under the conditions of a particular year of research. A uniform and clear division of the spectral characteristics of winter wheat according to the intensity of the disease was recorded only at a level of pathogen development of more than 5%. Moreover, this gradation was most clearly manifested in the spectral channels of the near-infrared range and at a wavelength of 720 nm.

Abstract The main aim of this report was to investigate and compare the response of serum C-reactive protein (CRP) and ferritin, two positive acute phase proteins (APPs) which usually show an increase in inflammatory processes, in dogs with pyometra. For this purpose, two different studies were made. In the first one , both proteins were measured together in an APPs profile in 25 dogs with pyometra, 25 dogs with pancreatitis (as an example of a positive inflammatory control group), and in 25 healthy dogs. In the second study, to advance the knowledge of the changes and evolution of serum ferritin and CRP in dogs with pyometra after treatment, the concentrations of both APPs were analyzed in 30 dogs with pyometra at diagnosis and after ovariohysterectomy and in 10 clinically healthy female dogs before and after elective spaying. In both studies, bitches with pyometra showed significant increases in serum CRP, indicating an inflammatory condition, but not in serum ferritin despite being a moderate positive APP. This divergence between the dynamics of these APPs could be a useful tool for the suspicion of cases of canine pyometra.

The vaginal tract comprises commensal microorganisms, which play an essential role in the health of the reproductive tract. Any dysbiosis in the vaginal microenvironment may lead to severe urinary tract infections or even infertility. This study aimed to evaluate the aerobic bacterial flora isolated from vaginal samples from 100 lactating bitches in the antepartum period (n = 3), postpartum period (n = 80), and with Lactatio sine graviditate (n = 17). Before vaginal swabs, all the bitches went through a gynecology consult, along with milk and blood sampling. Standard microbiological techniques were used for bacterial isolation. Among the 100 vaginal samples analyzed, 82% had a positive microbiological outcome, while 18% were negative. The microbiologic profile listed 17 different genera. The main isolated bacterial families were Micrococcaceae, Staphylococcaceae, Morganellaceae, Bacillaceae, and Rhizobiaceae. At the same time, strains like Agrobacterium radiobacter, Ochrobactrum anthropi, Chromobacterium violaceum, Burkholderia mallei, Bacillus pumllus, or Staphylococcus xylosus were isolated for the first time from the vaginal secretion of lactating bitches. The microbiological data demonstrates that lactating bitches’ vaginal discharge is heterogeneous and may be affected by coitus, sampling season, age, and reproductive status.

The aim of the study was to evaluate the concentrations of Interleukin-6 (IL-6) in milk and serum of healthy cows (HE) and cows with mastitis caused by Streptococcus spp. The blood and milk samples were obtained from Holstein-Friesian cows (Lublin region, Poland). A total of 43 milk and serum samples from 28 cows with mastitis and 15 healthy cows were selected for study. IL-6 levels in milk from HE cows ranged from 6.09–80.24 pg/mL (median 26.6 pg/mL) and were significantly lower than in milk from both cows with clinical and subclinical mastitis (487.09 pg/mL vs. 26.6 pg/mL in CM, p < 0.001; and 165.31 pg/mL vs. 26.6 pg/mL in SCM, p < 0.001). The IL-6 concentration in the serum of HE was not significantly different from the serum IL-6 of the entire group of mastitis cows, regardless of whether the inflammation proceeded in a clinical or subclinical form (44.37 pg/mL vs. 78.09 pg/mL; 128.29 pg/mL vs. 78.09 pg/mL, respectively). The present study indicates that cows with mastitis caused by Streptococcus spp. develop a local immune response in the mammary gland in response to the pathogen. Monitoring of IL-6 levels in milk can allow early detection of mastitis, which is especially important in cases of subclinical inflammation.

This study assessed the impact of a mixture of garlic (Allium sativum) and oregano (Origanum vulgare) essential oils (EOGOs) on in vitro dry matter digestibility (IVDMD) and in vivo apparent nutrient digestibility. Different EOGO inclusion levels were evaluated to assess the dose response and potential effects of the mixture. Three EOGO inclusion levels (0.5, 0.75, and 1 mL/kg of incubated dry matter) were evaluated in vitro, while four treatments (0.5, 0.75, and 1 mL/day of EOGO and a control group) were tested in vivo on 12 West African sheep. A randomized controlled trial was conducted using a 4 × 4 design. Blood parameters (glucose, blood urea nitrogen, and β-hydroxybutyrate) were measured to observe the effect of EOGO on the metabolism. The results showed that the inclusion of EOGO significantly enhanced IVDMD at low levels (p < 0.052) compared with the highest levels in treatments containing 0.5 and 0.75 mL/kg of EOGO dry matter. A higher intake of dry matter (DM), crude protein (CP), and neutral detergent fiber (NDF) (p < 0.05) was observed in the in vivo diets with the inclusion of EOGO. In terms of in vivo apparent digestibility, significant differences were found among treatments in the digestibility coefficients of DM, CP, and NDF. EOGO inclusion increased the digestibility of DM. CP digestibility displayed a cubic effect (p < 0.038), with the lowest values of digestibility observed at 1 mL EOGO inclusion. Additionally, NDF digestibility showed a cubic effect (p < 0.012), with the highest value obtained at 0.75 mL of EOGO inclusion. The inclusion levels above 0.75 mL EOGO showed a cubic effect, which indicates that higher concentrations of EOGO may not be beneficial for the digestibility of CP and NDF. Although no significant difference was observed in total digestible nutrients, a linear trend was observed (p < 0.059). EOGO improved the intake of DM, CP, and NDF. EOGO supplementation improved the digestibility of DM and NDF, with optimal levels observed at 0.5 mL/day. No significant effects were observed in the blood parameters. These results suggest that EOGO has the potential as an additive in ruminal nutrition to improve food digestibility and serve as an alternative to antibiotic additives. The use of EOGO potentially improves fiber digestion and may reduce the use of antibiotics in livestock production. Garlic (A. sativum) and oregano (O. vulgare) essential oils effectively modulated fiber digestibility at 0.75 mL/day. Garlic (A. sativum) and oregano (O. vulgare) essential oils have the potential to improve digestibility at low inclusion levels and serve as an alternative to antibiotic additives. The effectiveness of essential oils is greater in a mixture and at lower doses.

Abstract Background In severe cases, SARS-CoV-2 infection leads to acute respiratory distress syndrome (ARDS), often treated by extracorporeal membrane oxygenation (ECMO). During ECMO therapy, anticoagulation is crucial to prevent device-associated thrombosis and device failure, however, it is associated with bleeding complications. In COVID-19, additional pathologies, such as endotheliitis, may further increase the risk of bleeding complications. To assess the frequency of bleeding events, we analyzed data from the German COVID-19 autopsy registry (DeRegCOVID). Methods The electronic registry uses a web-based electronic case report form. In November 2021, the registry included N = 1129 confirmed COVID-19 autopsy cases, with data on 63 ECMO autopsy cases and 1066 non-ECMO autopsy cases, contributed from 29 German sites. Findings The registry data showed that ECMO was used in younger male patients and bleeding events occurred much more frequently in ECMO cases compared to non-ECMO cases (56% and 9%, respectively). Similarly, intracranial bleeding (ICB) was documented in 21% of ECMO cases and 3% of non-ECMO cases and was classified as the immediate or underlying cause of death in 78% of ECMO cases and 37% of non-ECMO cases. In ECMO cases, the three most common immediate causes of death were multi-organ failure, ARDS and ICB, and in non-ECMO cases ARDS, multi-organ failure and pulmonary bacterial ± fungal superinfection, ordered by descending frequency. Interpretation Our study suggests the potential value of autopsies and a joint interdisciplinary multicenter (national) approach in addressing fatal complications in COVID-19.

AIMS OF THE STUDY: Numerous studies from different countries have contributed to an improved understanding of blood culture-negative infective endocarditis. However, little is known about its epidemiology and microbiology in Switzerland. We aimed to assess the epidemiology and microbiology of blood culture-negative endocarditis at the University Hospital Zurich, Switzerland. METHODS: We screened all patients hospitalised between 1997 and 2020 with possible or definite endocarditis at our institution. Thereof, we identified all cases with blood culture-negative endocarditis and retrospectively retrieved patient characteristics, microbiological, histopathological, radiographic and surgical data from medical records. RESULTS: Among 861 patients screened, 66 (7.7%) cases of blood culture-negative endocarditis were identified. Thereof, 31 cases could be microbiologically documented or not documented (n = 30), and in five cases a non-infectious aetiology was confirmed. Endocarditis predominantly affected men (77%) and the left heart (79%); predisposing factors were prosthetic valves (42%), congenital heart disease (35%) and prior endocarditis (14%). The most common reasons for negative blood cultures were antibiotic treatment prior to blood culture sampling (35%), fastidious and slow growing microorganisms (30%) and definite non-infective endocarditis (8%). Coxiella burneti i and Bartonella spp. were the most common fastidious bacteria identified. In addition to serology, identification of causative microorganisms was possible by microbiological and/or histopathological analysis of tissue samples, of which polymerase chain reaction testing (PCR) of the 16S ribosomal RNA proved to be most successful. CONCLUSIONS: The present study provides a detailed analysis of blood culture-negative endocarditis over a time span of more than 20 years in Zurich, Switzerland. Antibiotic treatment prior to blood collection, and fastidious and slow growing organisms were identified as main reasons for sterile blood cultures. Typical culture-negative bacteria were mainly found by PCR and/or culture of tissue samples.

The development of remote methods for diagnosing the state of crops using spectral equipment for remote sensing of the Earth and original monitoring tools is the most promising solution to the problem of monitoring diseases of wheat agrocenoses. A research site was created on the experimental field of the Federal Research Center of Biological Plant Protection. Within the experimental field with a total area of 1 ha, test plots were allocated to create an artificial infectious background, and the corresponding control plots were treated with fungicides. The research methodology is based on the time synchronization of high-precision ground-based spectrometric measurements with satellite and unmanned remote surveys and the comparison of the obtained data with phytopathological field surveys. Our results show that the least-affected plants predominantly had lower reflectance values in the green, red, and red-edge spectral ranges and high values in the near-infrared range throughout the growing season. The most informative spectral ranges when using satellite images and multispectral cameras placed on UAVs are the red and IR ranges. At the same time, the high frequency of measurements is of key importance for determining the level of pathogenic background. We conclude that information acquisition density does not play as significant of a role as the repetition of measurements when carrying out ground-based spectrometry. The use of vegetation indices in assessing the dynamics of the spectral images of various survey systems allows us to bring them to similar values.

The results of laboratory experiments that show the capabilities of modern georadars are presented. Analysis of GPR capabilities allowed to propose a new approach to solving the actual problem of detecting subsurface cracks. The proposed method for recording pulse signals and data processing is based on algorithms and software previously developed by the authors. With the help of this method and the experiments carried out, the possibility of agreed solution to the problem of choosing the optimal parameters of the GPR pulsed signals was shown. To confirm the effectiveness of the developed signal processing methods, a series of laboratory experiments were carried out. The new version of the program "GeoVizy-2020" made it possible to increase the efficiency of processing complex impulse signals. To check the adequacy of the results obtained, a method was used, which is based on the procedure for direct measurement of the real layer thickness in laboratory and field conditions and comparison of the data obtained with the results of numerical simulation. As a result, evidence is presented of the advantages of using pulsed signals for assessing layer parameters, searching for subsurface cracks and processing the results of surveys of road surfaces is presented.

African swine fever (ASF) is a viral disease, endemic to Africa, that causes high mortality when introduced into domestic pig populations. Since the emergence of p72-genotype II African swine fever virus (ASFV) in Georgia in 2007, an ASF epidemic has been spreading across Europe and many countries in Asia. The epidemic first reached Ukraine in 2012. To better understand the dynamics of spread of ASF in Ukraine, we analyzed spatial and temporal outbreak data reported in Ukraine between 2012 and mid-2023. The highest numbers of outbreaks were reported in 2017 (N = 163) and 2018 (N = 145), with overall peak numbers of ASF outbreaks reported in August (domestic pigs) and January (wild boars). While cases were reported from most of Ukraine, we found a directional spread from the eastern and northern borders towards the western and southern regions of Ukraine. Many of the early outbreaks (before 2016) were adjacent to the border, which is again true for more recent outbreaks in wild boar, but not for recent outbreaks in domestic pigs. Outbreaks prior to 2016 also occurred predominantly in areas with a below average domestic pig density. This new analysis suggests that wild boars may have played an important role in the introduction and early spread of ASF in Ukraine. However, in later years, the dynamic suggests human activity as the predominant driver of spread and a separation of ASF epizootics between domestic pigs and in wild boars. The decline in outbreaks since 2019 suggests that the implemented mitigation strategies are effective, even though long-term control or eradication remain challenging and will require continued intensive surveillance of ASF outbreak patterns.

The aim of the publication is to design an analytical relationship for determining the bending coefficient measurable by belt friction. Following the results of the presented measurements, the theoretical assumption is verified that in the material of the belt stress arises during the friction of the contact surfaces, due to external forces and bending of the belt. Selected experiments of drawing a polypropylene belt at a constant speed over an aluminium cylindrical surface are carried out using a servo drive on a measuring bench with computer-controlled instrumentation. The results of friction and bending coefficient measurements are interpreted in connection with the setting of various input parameters (especially friction surfaces and belt thicknesses), at a constant shaft speed. The newly identified dimensionless bending coefficient of belt friction is a material coefficient with a comparative information value concerning the flexibility of the belt, suitably supplementing the friction Euler-Eytelwein coefficient. The presented publication can be suitably applied especially to the engineering educational process.

En condiciones del trópico colombiano es necesario establecer estrategias de alimentación que permitan aumentar la productividad a bajo costo. El objetivo de esta investigación fue evaluar el efecto de la suplementación con ensilados de totumo, mango, guayabo de monte sobre la digestibilidad in vitro, degradabilidad y parámetros productivos en vacas doble propósito. El estudio realizado en una finca ubicada, municipio de Puerto Boyacá, se usaron 32 vacas (Gyr x Holstein), distribuidas en cuatro (4) grupos de ocho (8) cada uno, conformados de la siguiente manera: T1: grupo control: sin ensilajes; T2: ensilaje de Guayabo de monte; T3: ensilaje de Totumo y T4: ensilaje de Mango común. Todas las vacas permanecían en pastoreo rotacional con Brachiaria decumbens, agua a voluntad y sal mineralizada. No hubo diferencias (P

Emerging RNA virus infections are a growing concern among domestic poultry industries due to the severe impact they can have on flock health and economic livelihoods. Avian paramyxoviruses (APMV; avulaviruses, AaV) are pathogenic, negative-sense RNA viruses that cause serious infections in the respiratory and central nervous systems. APMV was detected in multiple avian species during the 2017 wild bird migration season in Ukraine and studied using PCR, virus isolation, and sequencing. Of 4090 wild bird samples collected, mostly from southern Ukraine, eleven isolates were grown in ovo and identified for APMV serotype by hemagglutinin inhibition test as: APMV-1, APMV-4, APMV-6, and APMV-7. To build One Health’s capacity to characterize APMV virulence and analyze the potential risks of spillover to immunologically naïve populations, we sequenced virus genomes in veterinary research labs in Ukraine using a nanopore (MinION) platform. RNA was extracted and amplified using a multiplex tiling primer approach to specifically capture full-length APMV-1 (n = 5) and APMV-6 (n = 2) genomes at high read depth. All APMV-1 and APMV-6 fusion (F) proteins possessed a monobasic cleavage site, suggesting these APMVs were likely low virulence, annually circulating strains. Utilization of this low-cost method will identify gaps in viral evolution and circulation in this understudied but important critical region for Eurasia.

The accurate recognition of weeds on crops supports the spot application of herbicides, the high economic effect and reduction of pesticide pressure on agrocenoses. We consider the approach based on the quantitative spectral characteristics of plant objects to be the most appropriate for the development of methods for the spot application of herbicides. We made test plots with different species composition of cultivated and weed plants on the experimental fields of the scientific crop rotation of the Federal Research Center of Biological Plant Protection. These plants form the basis of the agrocenoses of Krasnodar Krai. Our primary subjects are sunflower crops (Helianthus annuus L.), corn (Zea mais L.) and soybean (Glycine max (L.)). Besides the test plots, pure and mixed backgrounds of weeds were identified, represented by the following species: ragweed (Ambrosia artemisiifolia L.), California-bur (Xanthium strumarium L.), red-root amaranth (Amaranthus retroflexus L.), white marrow (C. album L.) and field milk thistle (Sonchus arvensis L.). We used the Ocean Optics Maya 2000-Pro automated spectrometer to conduct high-precision ground-based spectrometric measurements of selected plants. We calculated the values of 15 generally accepted spectral index dependencies based on data processing from ground hyperspectral measurements of cultivated and weed plants. They aided in evaluating certain vegetation parameters. Factor analysis determined the relationship structure of variable values of hyperspectral vegetation indices into individual factor patterns. The analysis of variance assessed the information content of the indicators of index values within the limits of the selected factors. We concluded that most of the plant objects under consideration are characterized by the homogeneity of signs according to the values of the index indicators that make up the selected factors. However, in most of the cases, it is possible to identify different plant backgrounds, both by the values of individual vegetation indices and by generalized factorial coefficients. Our research results are important for the validation of remote aerospace observations using multispectral and hyperspectral instruments.

Leaf airborne diseases cause major shortfalls in agricultural crops. The introduction of technical means can significantly improve early-warning systems for plant diseases as well as provide timely and accurate forecasts. In this paper, we aim to evaluate the possibilities of detecting a phytopathogenic infection using a spore-catching device developed at the Federal Research Center of Biological Plant Protection (FRCBPP) on winter wheat varieties of different levels of susceptibility to major economically important leaf diseases, taking into account climatic conditions. The device captures spores in the surface layer of air among crop plants. We conducted research in the experimental fields of FRCBPP in 2019–2021. The objects of the study were four cultivars of winter wheat. They were selected according to the degree of resistance to various leaf diseases. We studied the progress of wheat diseases according to generally accepted international scales the onset of the first manifestations to their maximum development. We studied the aerogenic infection in wheat crops using the FRCBPP developed portable device for determining the infestation of plants. Sampling was carried out in the same period as the visual assessment. The samples were taken in the crops of each variety at five points. The sampling time was one minute. As a result of research on experimental crops of four varieties of winter wheat, we observed the development of such diseases as powdery mildew (Erysiphe graminis), yellow spot (Pyrenophora tritici-repentis), septoria leaf spot (Septoria tritici), yellow (Puccinia striiformis) and brown rust (Puccinia triticina). In a laboratory study of samples under a light microscope, all of the listed pathogens were found except for septoria leaf spot. Two-way analysis of variance confirmed the statistically significant separate and cumulative influence of the cultivar and year factor on winter wheat diseases. A generalized correlation analysis for three growing seasons (2019–2021) showed that an average statistically significant correlation coefficient (0.5–0.6) remains for the total groups for powdery mildew, yellow and brown rust. This indicator for the causative agent of yellow spot was equal to 0.4 with a high level of statistical significance. Thus, we conclude that by using a spore-catching device, it is possible to identify spores of economically significant pathogens in winter wheat crops and predict the further development of pathogens, taking into account the cultivar and annual climate factors.

We aimed to monitor the species diversity and the dynamics of the number of soybean pests using light traps with an original design to develop protection systems against the main phytophages. Traps lured 44 species of insects from eight orders and 27 families. The capture of 15 species of economically important phytophages was recorded—representatives of various orders and families: order Lepidoptera—Noctuidae, Crambidae, Erebidae, and Geometridae; order Hemiptera—Flatidae; order Coleoptera—Elateridae, etc. Insect identification was carried out via morphological methods. Over the study period (93 days), 4955.41 insect specimens were caught on average per one trap. Most of the attracted insects belong to harmful entomofauna: namely the cotton bollworm (Helicoverpa armigera, Hübner)—58.9%, the beet webworm (Loxostege sticticalis, L.)—12.74%, the nutmeg moth (Anarta trifolii, Hufnagel)—6.5%, the European corn borer (Ostrinia nubilalis, Hübner)—2.68%, and some other species—19.2%. In addition to economically significant phytophages, we registered some indifferent and beneficial species. The summer dynamics of the cotton bollworm and the nutmeg moth were obtained for the entire research period. Then, we calculated the values of the indices of biodiversity and the dominance of insect species. An analysis of the index values allows us to conclude a balanced entomocomplex at the research site.

Polyphenols (PPs) are a large group of phytochemicals containing phenolic rings with two or more hydroxyl groups. They possess powerful antioxidant properties, multiple therapeutic effects, and possible health benefits in vivo and in vitro, as well as reported clinical studies. Considering their free-radical scavenging and anti-inflammatory properties, these substances can be used to treat different kinds of conditions associated with metabolic disorders. Many symptoms of metabolic syndrome (MtS), including obesity, dyslipidemia, atherosclerosis, elevated blood sugar, accelerating aging, liver intoxication, hypertension, as well as cancer and neurodegenerative disorders, are substantially relieved by dietary PPs. The present study explores the bioprotective properties and associated underlying mechanisms of PPs. A detailed understanding of these natural compounds will open up new opportunities for producing unique natural PP-rich dietary and medicinal plans, ultimately affirming their health benefits.

Due to the growing demand for electrical energy generation worldwide [...]

Objetivo. Evaluar los efectos de diferentes niveles de concentrado fabricado con subproductos agrícolas del departamento del Huila-Colombia sobre el rendimiento y calidad de carne en bovinos confinados. Material y métodos. Se utilizaron 36 toretes F1 Bos taurus x Bos indicus con 347±20 kg de peso corporal y 18 meses de edad confinados bajo condiciones de bosque seco tropical. Los tratamientos experimentales fueron niveles crecientes de concentrado elaborado con subproductos agrícolas en sustitución de pasto Pennisetum spp a razón de 85% forraje:15% concentrado (T1); 75%forraje:25% concentrado (T2); 65% forraje:35% concentrado (T3) y 55%forraje:45% concentrado (T4). Se utilizó un diseño experimental completamente al azar para evaluar las eventuales diferencias entre variables evaluadas: ganancia de peso vivo (GPV), consumo de materia seca (CMS), conversión alimenticia (CA), peso de la canal caliente (PCC), rendimiento en canal (RC), metabolitos sanguíneos y perfil de ácidos grasos en la carne. Resultados. La GPV y el peso final aumentaron con un mayor nivel de concentrado en la dieta (p

Introducción: Las altas exigencias que presenta la carrera de medicina, aunado a las obligaciones laborales y personales de los estudiantes, los predispone a desarrollar ansiedad y síndrome de Burnout (SB), lo cual puede influir en su rendimiento académico. El objetivo del presente estudio fue determinar la prevalencia y la sintomatología de ansiedad y Burnout, así como identificar su impacto en el rendimiento académico de los estudiantes al término de la licenciatura en médico cirujano. Método: Se realizó un estudio transversal, observacional en estudiantes de medicina al término de la carrera, se utilizó el inventario de Ansiedad Rasgo y Estado, la escala Maslach Burnout y el promedio de las calificaciones a través de la Secretaria Académica. Resultados; Se observó una prevalencia de ansiedad estado (AE) y rasgo (AR) de 40.6% y 31.2% respectivamente, ambas con proporciones similares entre nivel medio (75%) y alto (25%), y con asociación entre las mismas. La prevalencia del SB fue de 15.6%, de los cuales 17.2 y 23.4% presentaron niveles altos de cansancio y de despersonalización respectivamente, además 18.8% mostró niveles bajos de realización personal. Conclusión: Al término de la licenciatura los estudiantes muestran sintomatología de AR y AE con alta asociación entre ambas, por lo que es importante resaltar que la ansiedad transitoria puede llegar a integrar una respuesta ansiosa como rasgo de su personalidad. La prevalencia de SB fue baja y sin relación con el rendimiento académico, la edad o el sexo.

Aleutian disease (AD) is a chronic disease of mink caused by the Aleutian Mink Disease Virus (AMDV) that results in dysfunction of the immune system. The prevalence of asymptomatic AMDV infections suggests a necessity to explore their effects on the cellular mechanisms of non-specific immunity in farmed mink. The study evaluated the phagocytic activity and oxygen metabolism of peripheral blood granulocytes and monocytes in mink with chronic subclinical AMDV infection. Moreover, the intensity of inflammatory processes was assessed based on the serum amyloid A (SAA) concentration. The analyses involved 24 brown mink females aged 12–24 months. The experimental group (group I) consisted of mink with chronic subclinical AMDV infections, and the control group (group II) included healthy animals. The statistical analysis was performed using the Mann-Whitney U rank test. Phagocytic activity of granulocytes and monocytes was carried out using flow cytometry, and SAA concentration was determined with enzyme-linked immunosorbent assay (ELISA). Compared with the control group, there was a significant decrease in the phagocytic activity and oxygen metabolism of granulocytes and monocytes in the AMDV-infected mink (p < 0.05). Additionally, it was found that the mean SAA value was significantly higher in the group infected with AMDV than in the control group (p < 0.05). The obtained data indicate that monitoring the serum SAA levels in mink with asymptomatic inflammation may help assess the health of mink and detect asymptomatic inflammation caused by AMDV infection.

The necessity of increasing the efficiency of primary oil purification in a drilling station or an offshore platform has been substantiated. We consider the problems that arise during the primary processing of oil mixtures. Important conditions for increasing the efficiency of primary purification (separation) of oil mixtures include measuring the consumption and determining the content of various impurities (water, undissolved particles) and air in them, with an error of no more than 2%. We analyzed the possibilities of using various designs of flowmeters to measure the consumption of the oil mixture coming from a well. It is also necessary to use other measuring instruments to control the state of this mixture, which creates additional problems (searching for an appropriate locations to place them, providing the required operating conditions). Various designs of nuclear magnetic flowmeters–relaxometers were considered, making it possible to measure the consumption of a liquid medium and its times of longitudinal T1 and transverse T2 relaxation with one device. The measured values of T1 and T2 determine the state of the medium. The design of the industrial nuclear magnetic flowmeter–relaxometer M-Phase 5000, which is used to control the flow and quality of oil and oil products, was considered in more detail. Problems were identified that did not allow using this design of a nuclear magnetic flowmeter–relaxometer in a drilling rig or offshore platform. A new design of a nuclear magnetic flowmeter–relaxometer was developed, implementing the methods for measuring q, T1, and T2. These methods and various technical solutions make it possible to use this device at a drilling station or offshore platform. The measurement errors of the consumption q, T1, and T2 were determined. The results of various media studies are presented and compared with q, T1, and T2 measurements on other devices and measured volume (to confirm the adequacy of q measurements). The application scopes of the developed nuclear magnetic flowmeter–relaxometer were determined, in addition to the systems of primary oil processing.

de Luis Roman DA, Bachiller P, Izaola O, et al. Eur J Clin Nutr 2001;55:1048-1052. OBJECTIVE: Dietary counseling and intervention based on application of conventional criteria have been ineffective in [...]

Objective: to evaluate the effects of organic copper (Cu) and selenium (Se) on milk production, milk quality and reproductive parameters of Normande dairy cows. Materials and methods: 36 multiparous cows (BW = 600 ± 32 kg) were used, distributed in completely randomized blocks. Treatments were: T0: without mineral supplementation, T1: inorganic mineral supplementation with Cu and Se, T2: 50% Inorganic supplementation + 50% organic supplementation, with an experimental period of 150 days (30 days before calving and 120 days of lactation). The variables evaluated were milk production and quality (total solids, proteins, fat, somatic cells and MUN), blood metabolites (glucose, betahydroxybutyrate and BUN) and reproductive parameters (open days, days to first service and services per conception). Results: No differences were observed in milk production and quality (P> 0.05). However, open days and services per conception were shorter when cows were supplemented with organic minerals (P

High forage diets in the tropics are less efficient and more contaminating due to high energy losses as methane and the low digestibility of tropical forages. The objective of the study was to determine the effects of essential oils (EO) of Anacardium occidentale and Ricinus communis as additives in high forage diets on intake, digestibility, ruminal fermentation and synthesis of microbial protein. Four ‘Holstein’ steers fitted with ruminal cannula were used in a 4 × 4 Latin square design (21-day period). The treatments were 1, 2, 4 and 8 g day-1 EO per animal (essential oils, Oligobasics®). The diet was total mixed ration (TMR) of Brachiaria hay (Brachiaria humidicola cv. Lanero) and concentrate (ground corn, soybean meal, ammonium sulphate, urea and minerals) in an 80:20 ratio. Differences were observed in the digestibility of neutral detergent fibre (NDF) and total digestible nutrients (TDN) in levels above 2 g day -1 EO (P < 0.05). The VFA (volatile fatty acids) acetate and propionate were higher in diets with 2 g day -1 EO (P < 0.05), without affecting the acetate:propionate ratio. Levels above 2 g day -1 EO negatively affected the faecal N excretion (P < 0.05). The inclusions of A. occidentale and R. communis EOs at 2 g day-1 per day animal improved fibre digestion and decreased N excretion. These results indicate that this mixture of EOs may have strong antimicrobial activity at lower doses. The N losses, fibre digestion and digestibility could be improved with the use of cashew and castor essential oils in high forage diets.

The present study evaluated for the first time changes in the saliva proteome in bitches with pyometra through a high-throughput quantitative proteomic analysis. The aims were to explore whether saliva composition could reflect the physiopathological changes occurring in canine pyometra and to identify potential biomarkers of the disease. Saliva samples from six healthy (H) and six bitches with pyometra (P) were analyzed using tandem mass tags–based approach. Additionally, 15 samples were used for the validation of changes in haptoglobin (Hp) concentration in saliva of dogs with pyometra. Proteomic analysis quantified 707 proteins in saliva. Comparison of the two groups revealed 16 unique proteins significantly modulated in saliva, with S100A calcium-binding protein 12 (S100A12), vimentin, and Hp the most up-regulated in canine pyometra. According to PANTHER (Protein Analysis Through Evolutionary Relationships) classification tool, these proteins are mainly related to proinflammatory mediators, acute-phase proteins, and sepsis. In conclusion, it can be stated that there are changes in various proteins in saliva in canine pyometra reflecting different physiopathological changes occurring in this disease. These proteins could be a source of potential non-invasive biomarkers for this disease that should be confirmed in future studies.

The necessity of coolant flow consumption measurement accuracy increase in the nuclear reactor primary circuit has been substantiated. Additionally, the need to control the coolant condition in the current flow inside the pipeline is shown. Nowadays, the real-time coolant’s condition control function is not implemented at stationary nuclear power plants or mobile nuclear power plants used in moving objects. It is shown that a coolant consumption measurement error decreases and its condition data availability increases the heat transfer efficiency and the electrical energy generation (without the nuclear reactor and steam generator design change). Problems arising during coolant consumption control using various flowmeters models in the nuclear reactor primary circuit are considered. It has been found that nuclear magnetic flowmeters can solve these problems. New difficulties are noted as emerging when using pulsed nuclear magnetic flowmeters designs developed for measuring hydrocarbons, water, biological compounds consumption, and condition control. A new nuclear magnetic flowmeter design has been developed using a modulation technique for nuclear magnetic resonance signal recording. Methods for measuring the coolant flow’s longitudinal T1 and transverse T2 relaxation times are presented. Investigations of coolant flow parameters (consumption and relaxation times) inside the pipeline have been carried out. It is found that the measurement error for these parameters does not exceed 1%. The prospects of using the developed nuclear magnetic flowmeter-relaxometer design in the nuclear reactor first circuit are shown.

The necessity to control the feed water state in the second loop of a nuclear power plant nuclear reactor is justified. The different methods of the state control of flowing water in the pipeline are reviewed. It has been established that controlling the feed water state should not result in irreversible changes in its chemical composition and physical structure. A change in the composition or structure of feed water leads to a change in its heat capacity. The heat transfer deteriorates, the production of electrical energy in the installation decreases, and the additional release of heat into the atmosphere increases. This process also occurs during the heat capacity changes for other reasons. The method for controlling the feed water heat capacity by measuring the value of the refractive index n is developed. The design of a flow-through refractometer based on the total internal reflection for control of the feed water state in the stream is made. The dependence of the heat capacity change of feed water from the refractive index is established. The results of research on different types of water are presented.

Abstract Background The degree of differentiation of mast cell tumours (MCTs) is the most important feature and reflects the morphological characteristics and metastatic potential of the tumour and its likely response to treatment and the prognosis. The aim of this study was to epidemiologically analyse the risk of MCT development in dogs according to breed, age, sex, size and anatomical location of the tumour using the Kiupel grading system. The analysis involved 492 dogs selected based on a histopathological assessment of 2763 canine skin tumours. A logistic regression analysis was performed to determine the odds ratios (ORs) with 95% confidence intervals. Results Mast cell tumours accounted for 17.8% of all diagnosed canine skin tumours. The highest risk of high-grade MCTs was noted in the Shar-Pei (OR 28.18, P