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1. In vivo analysis of the stability and transport of nuclear poly(A)+ RNA

Author

Huang, Sui, Deerinck, Thomas J., Ellisman, Mark H., and Spector, David L.

Subjects
RNA -- Romania, Biological transport -- Analysis, Biological sciences
Abstract

Salt and detergent extraction, suppression of RNA polymerase II transcription and DNase I digestion result in stable poly(A)+ RNA elements in the nucleus of mammalian cells. The stable RNA elements regroup into small and large chromatin granule clusters containing pre-mRNA splicing factors. The poly(A)+RNA transportation to the nuclear pore complexes occurs through a controlled pathway.

Published
1994

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