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4 results on '"RLFPs"'

1. Increased genetic diversity in Greek populations of the genus Ligidium (Crustacea: Isopoda: Oniscidea) revealed by RFLP analysis of mtDNA segments

Subjects
12S rDNA, genetic isolation, Greece, RLFPs, mtDNA, isopods, 16S rDNA, Ligidium, Oniscidea
Abstract

We investigated mtDNA genetic differentiation and the phylogenetic relationships of 11 populations of the oniscidean genus Ligidium. We studied nine populations from Greece, assigned to three nominal species (L. euboicum, L. germanicum and L. beieri), and two from central Europe (L. germanicum and L. hypnorum), applying RFLP analysis of two mtDNA segments (12S rDNA and 16S rDNA) amplified by PCR. The analysis revealed seven monomorphic and four polymorphic populations, and 17 different haplotypes, each one present in one population only. Several restriction enzymes produced population specific patterns, enabling discrimination among the different populations. The estimated net nucleotide divergence between populations ranges from 1.06 to 16.52%, while the high NST value (0.96) indicates the existence of increased interpopulation genetic differentiation that can be attributed to the extreme isolation among Ligidium populations. The relationships between haplotypes and populations are not congruent with their geographical distribution and the established taxonomy within the genus.

Published
2005

2. Increased genetic diversity in Greek populations of the genus Ligidium (Crustacea: Isopoda: Oniscidea) revealed by RFLP analysis of mtDNA segments

Author

Klossa-Kilia, E., Kilias, G., Sfenthourakis, Spyros, and Sfenthourakis, Spyros [0000-0003-3213-2502]

Subjects
12S rDNA, Greece, mtDNA, Ligidium euboicum, Ligidium hypnorum, Oniscidea, Isopods, Ligidium germanicum, Ligidium beieri, RLFPs, Crustacea, 16S rDNA, Ligidium, Genetic isolation, Isopoda
Abstract

We investigated mtDNA genetic differentiation and the phylogenetic relationships of 11 populations of the oniscidean genus Ligidium. We studied nine populations from Greece, assigned to three nominal species (L. euboicum, L. germanicum and L. beieri), and two from central Europe (L. germanicum and L. hypnorum), applying RFLP analysis of two mtDNA segments (12S rDNA and 16S rDNA) amplified by PCR. The analysis revealed seven monomorphic and four polymorphic populations, and 17 different haplotypes, each one present in one population only. Several restriction enzymes produced population specific patterns, enabling discrimination among the different populations. The estimated net nucleotide divergence between populations ranges from 1.06 to 16.52%, while the high NST value (0.96) indicates the existence of increased interpopulation genetic differentiation that can be attributed to the extreme isolation among Ligidium populations. The relationships between haplotypes and populations are not congruent with their geographical distribution and the established taxonomy within the genus. 74 255 264 Cited By :6

Published
2006

3. Increased genetic diversity in Greek populations of the genus Ligidium (Crustacea: Isopoda: Oniscidea) revealed by RFLP analysis of mtDNA segments

Author

Klossa-Kilia, E., Kilias, G., Sfenthourakis, S., Klossa-Kilia, E., Kilias, G., and Sfenthourakis, S.

Abstract

We investigated mtDNA genetic differentiation and the phylogenetic relationships of 11 populations of the oniscidean genus Ligidium. We studied nine populations from Greece, assigned to three nominal species (L. euboicum, L. germanicum and L. beieri), and two from central Europe (L. germanicum and L. hypnorum), applying RFLP analysis of two mtDNA segments (12S rDNA and 16S rDNA) amplified by PCR. The analysis revealed seven monomorphic and four polymorphic populations, and 17 different haplotypes, each one present in one population only. Several restriction enzymes produced population specific patterns, enabling discrimination among the different populations. The estimated net nucleotide divergence between populations ranges from 1.06 to 16.52%, while the high NST value (0.96) indicates the existence of increased interpopulation genetic differentiation that can be attributed to the extreme isolation among Ligidium populations. The relationships between haplotypes and populations are not congruent with their geographical distribution and the established taxonomy within the genus.

Published
2005

4. Increased genetic diversity in Greek populations of the genus Ligidium (Crustacea: Isopoda: Oniscidea) revealed by RFLP analysis of mtDNA segments

Author

Spyros Sfenthourakis, George Kilias, E. Klossa-Kilia, and Naturalis journals & series

Subjects
12S rDNA, Genetics, education.field_of_study, Genetic diversity, genetic isolation, Greece, biology, Phylogenetic tree, mtDNA, isopods, Ligidium, Population, Oniscidea, biology.organism_classification, Isopoda, RLFPs, Evolutionary biology, 16S rDNA, Animal Science and Zoology, Taxonomy (biology), Restriction fragment length polymorphism, education, Genetic isolate, Ecology, Evolution, Behavior and Systematics
Abstract

We investigated mtDNA genetic differentiation and the phylogenetic relationships of 11 populations of the oniscidean genus Ligidium. We studied nine populations from Greece, assigned to three nominal species (L. euboicum, L. germanicum and L. beieri), and two from central Europe (L. germanicum and L. hypnorum), applying RFLP analysis of two mtDNA segments (12S rDNA and 16S rDNA) amplified by PCR. The analysis revealed seven monomorphic and four polymorphic populations, and 17 different haplotypes, each one present in one population only. Several restriction enzymes produced population specific patterns, enabling discrimination among the different populations. The estimated net nucleotide divergence between populations ranges from 1.06 to 16.52%, while the high NST value (0.96) indicates the existence of increased interpopulation genetic differentiation that can be attributed to the extreme isolation among Ligidium populations. The relationships between haplotypes and populations are not congruent with their geographical distribution and the established taxonomy within the genus.

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