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Foi testada metodologia que permite o acompanhamento de nódulos individuais de leguminosas ao longo do tempo. Dos sete dias da emergência até a maturidade fisiológica de plantas de caupi, realizaram-se determinações semanais de número, tamanho, matéria seca dos nódulos, área foliar, altura da planta, matéria seca da parte aérea e do sistema radicular e atividade da nitrogenase. Os nódulos foram digitalizados nas resoluções de 100, 200 e 300 pontos porpolegada, e o comprimento e diâmetro de cada nódulo foram determinados com paquímetro digital e nas imagens digitais. Obtiveram-se imagens do sistema radicular de todas as plantassemanalmente na resolução de 300 ppp nos lados mais extensos do vaso. As medições destrutivas e não destrutivas de tamanho de nódulos geralmente apresentaram-se similares. Medições não destrutivas de tamanho de nódulos demonstraram-se como preditoras válidas para medidas como matéria seca de nódulos.A method was tested to allow the study over time of individual legumenodules. From seven days after emergence to full physiological maturity of cowpea plants weekly determinations of nodule number, size and dry matter, leaf area, plant height aerial part and root system dry masses and nitrogenase activity. Nodules were digitized at 100, 200 and 300 dots per inch resolutions, and each nodule length and diameter was measured with a digital pachymeter and on the digital images. Images of the root system of all plants were obtainedweekly at 300 dpi on the larger sides of the pots. Destructive and non-destructive nodule size determinations were generally similar. Non-destructive measures of nodule size were valid predictors for measures such as nodule dry matter.

Biofertilizers use in agriculture allow to ensure culture productivity, reducing chemical fertilizers and cost. Azofert® is a bioprepared composed by rizobia strains, with a positive effect on biological nitrogen fixation. Biobras-16 and Quitomax are natural bioactive products, considered as biostimulants which help plant to face various kinds of stresses and increase yields; however there is no information about the effect of the application of these biostimulants to seeds before biofertilizer inoculation on bean crop. The objective of this work was to determinate the Biobras 16 and Quitomax effect on nodulation and growth of common bean biofertilized with Azofert®. Two concentrations of Quitomax and Biobras-16 were applied on seeds the day before sowing and with Azofert® at the sowing moment, then they were put on Ferralític Red Lixiviated típic éutrico soil. 35 days after sowing were determined in ten plants by treatment: number and dry weight of nodules, shoot and root length and dry weight. Determination of leaf chlorophyll, nitrogen and phosphorus were also made. The results showed that application of specific concentration of Biobras-16 and Quitomax plus Azofer®t inoculation; stimulate, in a general way, the growth of plants; mainly with Biobras-16, which also increased leaf chlorophyll and nitrogen contents. [ABSTRACT FROM AUTHOR]