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Although Campylobacter jejuni is the pathogen responsible for the most common foodborne illness, tracing of the infection source remains challenging due to its highly variable genome. Therefore, one of the aim of the study was to compare three genotyping methods (MLST, PFGE, and mP-BIT) to determine the most effective genotyping tool. C. jejuni strains were divided into 4 clusters based on strain similarity in the cgMLST dendrogram. Subsequently, the dendrograms of the 3 tested methods were compared to determine the accuracy of each method compared to the reference cgMLST method. Moreover, a cost-benefit analysis has showed that MLST had the highest inverse discrimination index (97%) and required less workflow, time, fewer consumables, and low bacterial sample quantity. PFGE was shown to be obsolete both because of its low discriminatory power and the complexity of the procedure. Similarly, mP‑BIT showed low separation results, which was compensated by its high availability. Therefore, our data showed that MLST is the optimal tool for genotyping C. jejuni. Another aim was to compare the antimicrobial resistance to ciprofloxacin, erythromycin, and tetracycline in C. jejuni strains isolated from human, water, air, food, and animal samples by two gene sequence-based prediction methods and to compare them with the actual susceptibility of C. jejuni strains using the disc diffusion method. Both tools, ResFinder and RGI, synchronously predict the antimicrobial susceptibility of C. jejuni and either can be used. [ABSTRACT FROM AUTHOR]

Alpine glaciers are a fundamental component of the cryosphere and are significantly sensitive to climate change. One such region is the Hindukush Karakoram Himalaya (HKH) and Tibetan Plateau (TP) region, which contains more than 40,000 glaciers. There are more than 12 glacier inventories available covering parts of (or the entire) HKH region, but these show significant uncertainties regarding the extent of glaciers. Researchers have used different glacier inventories without assessing their accuracy. This study, therefore, assessed the implications of the accuracy of global glacier inventories in hydrological modeling and future water resource planning. The accuracy assessment of most commonly used two global glacier inventories (Global Land Ice Monitoring from Space-GLIMS v 2.0 and Randolph Glacier Inventory-RGI v 6.0) has been carried out for three sub-basins of the Upper Indus Basin—the Swat, the Chitral, and the Kabul River basins (combined, this is referred to as the Great Kabul River Basin)—with a total basin area of 94,552.86 km2. Glacier outlines have been compared with various Landsat 7 ETM+, Landsat 8, high-resolution Google Earth images, and manually digitized debris-covered glacier outlines during different years. The total glacier area for the Great Kabul River Basin derived from RGI and GLIMS is estimated to be 2120.35 km2 and 1789.94 km2, respectively, which was a difference of 16.9%. Despite being sub-basins of the Great Kabul River Basin, the Swat, and the Chitral River basins were different by 54.74% and 19.71%, respectively, between the two inventories, with a greater glacierized area provided by RGI, whereas the Kabul River basin was different by 54.72%, with greater glacierized area provided by GLIMS. The results and analysis show that GLIMS underestimates glacier outlines in the Swat and the Chitral basins and overestimates glacier extents in the Kabul River basin. The underestimation is mainly due to the non-representation of debris-covered glaciers. The overestimation in GLIMS data is due to the digitization of seasonal snow as part of the glaciers. The use of underestimated GLIMS outlines may result in 5–10% underestimation of glacier-melt contribution to flows in the Swat River basin, while an underestimation of 7% to 15% is expected in the Chitral River Basin, all compared to RGI v 6.0 outlines. The overestimation of glacier-melt contribution to flows in the Kabul River basin is insignificant (1% to 2%) using GLIMS data. In summary, the use of the GLIMS inventory will lead to underestimated flows and show that the Great Kabul River Basin (particularly the Chitral River Basin) is less sensitive to climate change effects. Thus, the current study recommends the use of RGI v 6.0 (best glacier inventory) to revisit the existing biased hydro-climate studies and to improve future hydro-climate studies with the concomitant rectification of the MODIS snow coverage data. The use of the best glacier inventory will provide the best estimates of flow sensitivity to climate change and will result in well-informed decision-making, precise and accurate policies, and sustainable water resource management in the study area. The methodology adopted in the current study may also be used in nearby areas with similar hydro-climate conditions, as well as for the most recently released RGI v 7.0 data. [ABSTRACT FROM AUTHOR]

The rapid urbanization in recent decades has highlighted the impact of rural land utilization, which further affects the spatial structure and efficiency of rural ecosystems. Optimizing the structure of urban green infrastructure is an effective way to alleviate the fragmentation of rural landscapes, coordinate the relationship between rural development and ecosystem services, and ensure sustainable rural development. The purpose of this paper is to provide a clear direction for the optimization of construction for the sustainable development of rural green infrastructure (RGI). This study provides a new method for RGI identification and analysis by taking Suining County, a typical rural area on the North China Plain, as an example. Morphological spatial pattern analysis (MSPA) was used to distinguish different space scenery elements in RGI and combine them with land utilization elements, so as to obtain two types of overlapping degree data in each village and town. We further combined the overlapping degree data with ecological efficiency indicators to evaluate the spatial structure construction priorities of different land use components in the RGI system. The results show that the MSPA and ecological efficiency analysis method proposed in this paper are conducive to qualitative and quantitative analysis of the relationship between land use type, spatial structure, and ecological efficiency in the RGI system and are suitable for the construction of a green infrastructure network. This method can be used to better understand the spatial distribution and priority of green infrastructure networks to achieve sustainable rural development on the North China Plain. [ABSTRACT FROM AUTHOR]

Main conclusion: PAE8 and PAE9 have pectin acetylesterase activity and together remove one-third of the cell wall acetate associated with pectin formation in Arabidopsis leaves. In pae8 and pae9 mutants, substantial amounts of acetate accumulate in cell walls. In addition, the inflorescence stem height is decreased. Pectic polysaccharides constitute a significant part of the primary cell walls in dicotyledonous angiosperms. This diverse group of polysaccharides has been implicated in several physiological processes including cell-to-cell adhesion and pathogenesis. Several pectic polysaccharides contain acetyl-moieties directly affecting their physical properties such as gelling capacity, an important trait for the food industry. In order to gain further insight into the biological role of pectin acetylation, a reverse genetics approach was used to investigate the function of genes that are members of the Pectin AcetylEsterase gene family (PAE) in Arabidopsis. Mutations in two members of the PAE family ( PAE8 and PAE9) lead to cell walls with an approximately 20 % increase in acetate content. High-molecular-weight fractions enriched in pectic rhamnogalacturonan I (RGI) extracted from the mutants had increased acetate content. In addition, the pae8 mutant displayed increased acetate content also in low-molecular-weight pectic fractions. The pae8/pae9- 2 double mutant exhibited an additive effect by increasing wall acetate content by up to 37 %, suggesting that the two genes are not redundant and act on acetyl-substituents of different pectic domains. The pae8 and pae8/ pae9- 2 mutants exhibit reduced inflorescence growth underscoring the role of pectic acetylation in plant development. When heterologously expressed and purified, both gene products were shown to release acetate from the corresponding mutant pectic fractions in vitro. PAEs play a significant role in modulating the acetylation state of pectic polymers in the wall, highlighting the importance of apoplastic metabolism for the plant cell and plant growth. [ABSTRACT FROM AUTHOR]

Background and Aims Pectin is a complex macromolecule, the fine structure of which is influenced by many factors. It is used as a gelling, thickening and emulsifying agent in a wide range of applications, from food to pharmaceutical products. Current industrial pectin extraction processes are based on fruit peel, a waste product from the juicing industry, in which thousands of tons of citrus are processed worldwide every year. This study examines how pectin components vary in relation to the plant source (orange, lemon, lime, grapefruit) and considers the influence of extraction conditions on the chemical and macromolecular characteristics of pectin samples. Methods Citrus peel (orange, lemon, lime and grapefruit) from a commercial supplier was used as raw material. Pectin samples were obtained on a bulk plant scale (kilograms; harsh nitric acid, mild nitric acid and harsh oxalic acid extraction) and on a laboratory scale (grams; mild oxalic acid extraction). Pectin composition (acidic and neutral sugars) and physicochemical properties (molar mass and intrinsic viscosity) were determined. Key Results Oxalic acid extraction allowed the recovery of pectin samples of high molecular weight. Mild oxalic acid-extracted pectins were rich in long homogalacturonan stretches and contained rhamnogalacturonan I stretches with conserved side chains. Nitric acid-extracted pectins exhibited lower molecular weights and contained rhamnogalacturonan I stretches encompassing few and/or short side chains. Grapefruit pectin was found to have short side chains compared with orange, lime and lemon. Orange and grapefruit pectin samples were both particularly rich in rhamnogalacturonan I backbones. Conclusions Structural, and hence macromolecular, variations within the different citrus pectin samples were mainly related to their rhamnogalacturonan I contents and integrity, and, to a lesser extent, to the length of their homogalacturonan domains. [ABSTRACT FROM PUBLISHER]

It has been reported that modified forms of pectin possess anticancer activity. To account for this bioactivity, it has been proposed that fragments of pectin molecules can act by binding to and inhibiting the various roles of the mammalian protein galectin 3 (Gal3) in cancer progression and metastasis. Despite this clear molecular hypothesis and evidence for the bioactivity of modified pectin, the structural origins of the "bioactive fragments" of pectin molecules are currently ill defined. By using a combination of fluorescence microscopy, flow cytometry, and force spectroscopy, it has been possible to demonstrate, for the first time, specific binding of a pectin galactan to the recombinant form of human Gal3. Present studies suggest that bioactivity resides in the neutral sugar side chains of pectin polysaccharides and that these components could be isolated and modified to optimize bioactivity. [ABSTRACT FROM AUTHOR]

Defatted Sesamum indicum seed cake was extracted, following two separate sequences, and the effects of extraction medium on yield and composition of the extracts were compared. Polysaccharides extracted sequentially with dilute acid and alkali represented 250 mg/g of defatted meal. The isolated polymers contained arabinan, rhamnogalacturonan I (RG I) and arabinogalactan proteins. Polysaccharides extracted during chlorite treatment and with dilute alkali had a higher proportion of rhamnose, suggesting a more branched variety of polymer. Three extracts, which were further characterized by size exclusion chromatography, gave two overlapping peaks. Structural characterisation of hemicellulosic polysaccharides, isolated with KOH, using specific enzyme hydrolysis, ion exchange chromatography (HPAEC) and matrix assisted laser desorption ionisation-time-of-flight (MALDI-TOF) mass spectroscopy, showed that sesame meal xyloglucan (XG) contained XXXG, XXFG and XXLG, and XLLG (named according to ) as the major building sub-units in the ratio of 1:0.9:0.3. Hydrolysis with endo-β-(1 → 4)-d-xylanase and analysis of the xylan derived oligosaccharides showed the presence of monomeric xylose (40%), xylobiose (46%) and acidic xylan oligosaccharides containing 4-O-methyl-d-glucuronic acid residues (14%). [Copyright &y& Elsevier]