1. Sgk1 enhances RANBP1 transcript levels and decreases taxol sensitivity in RKO colon carcinoma cells
- Author
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Rodolfo Iuliano, Domenica Scumaci, Rosario Amato, Valter Agosti, Adriana Zingone, Anna Maria Mileo, M. Di Sanzo, Patrizia Lavia, Emma Colao, Florian Lang, Lucia D'Antona, Maria Concetta Faniello, Francesco Costanzo, Nicola Perrotti, Paola Malatesta, Giovanni Cuda, Miranda Menniti, and Marco G. Paggi
- Subjects
Proteomics ,Cancer Research ,Cancer therapy ,Paclitaxel ,Transcription, Genetic ,Sp1 Transcription Factor ,Apoptosis ,Biology ,Protein Serine-Threonine Kinases ,Immediate-Early Proteins ,Growth factor receptor ,RAN-binding protein 1 ,RNA interference ,Cell Line, Tumor ,Genetics ,Gene silencing ,Humans ,Phosphorylation ,Molecular Biology ,taxol ,Effector ,Cell growth ,urogenital system ,Carcinoma ,Nuclear Proteins ,Cell cycle ,Cell biology ,Gene Expression Regulation, Neoplastic ,RNA silencing ,Drug Resistance, Neoplasm ,Sgk1 kinase ,Cancer cell ,Colonic Neoplasms ,Cancer research ,RNA Interference - Abstract
The serum- and glucocorticoid-regulated kinase (Sgk1) is essential for hormonal regulation of epithelial sodium channel-mediated sodium transport and is involved in the transduction of growth factor-dependent cell survival and proliferation signals. Growing evidence now points to Sgk1 as a key element in the development and/or progression of human cancer. To gain insight into the mechanisms through which Sgk1 regulates cell proliferation, we adopted a proteomic approach to identify up- or downregulated proteins after Sgk1-specific RNA silencing. Among several proteins, the abundance of which was found to be up- or downregulated upon Sgk1 silencing, we focused our attention of RAN-binding protein 1 (RANBP1), a major effector of the GTPase RAN. We report that Sgk1-dependent regulation of RANBP1 has functional consequences on both mitotic microtubule activity and taxol sensitivity of cancer cells.
- Published
- 2012
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