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1. Tay-Sachs' and Sandhoff's diseases: the assignment of genes for hexosaminidase A and B to individual human chromosomes

Author

Raju Kucherlapati, M. J. Murnane, Frank H. Ruddle, R.P. Creagan, Gretchen J. Darlington, and F. Gilbert

Subjects
Male, Genetic Linkage, Pyruvate Kinase, Somatic Cell Genetics, Hybrid Cells, Biology, Lipidoses, Cell Line, Mice, Genetic linkage, Animals, Humans, Hexosaminidase, Chromosomes, Human, 4-5, Gene, Synteny, Genetics, Multidisciplinary, Autosome, Chromosome Mapping, Chromosome, Molecular biology, Hexosaminidase B, Clone Cells, Hexosaminidases, Genes, Female, Carbohydrate Epimerases, Mannose, Research Article
Abstract

The techniques of somatic cell genetics have been used to establish the linkage relationships of loci coding for two forms (A and B) of hexosaminidase (EC 3.2.1.30; 2-acetamido-2-deoxy-beta-D-glucoside acetamidodeoxyglucohydrolase) and to determine whether a structural relationship exists between these forms. In a series of human-mouse hybrid cell lines, hexosaminidase A and B segregated independently. Our results and those reported by other investigators are used to analyze the proposed structural models for hexosaminidase. We have also been able to establish a syntenic relationship between the gene locus responsible for the expression of hexosaminidase A and those responsible for mannosephosphate isomerase and pyruvate kinase-3 and to assign the gene for hexosaminidase B to chromosome 5 in man. There is thus a linkage between specific human autosomes and enzymes implicated in the production of lipid storage diseases.

Published
1975
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2. Assignment of the gene for galactokinase to human chromosome 17 and its regional localisation to band q21-22

Author

Raju Kucherlapati, E.A. Nichols, Richard E. Giles, Frank H. Ruddle, K. Willecke, R.P. Creagan, S.M. Elsevier, and J.K. McDougall

Subjects
Genetics, Multidisciplinary, Phosphotransferases, Somatic Cell Genetics, Chromosome Mapping, Chromosome, Hybrid Cells, Biology, Galactokinase, Cell Line, Clone Cells, Chromosome 17 (human), Leloir pathway, Mice, Genes, Thymidine kinase, Animals, Humans, Human genome, Gene, Chromosomes, Human, 16-18
Abstract

PROGRESS in somatic cell genetics now allows more rapid and precise localisation of genes within the human genome. A large number of mouse × human hybrid cell lines are available, permitting the investigator to choose, for phenotype assay, lines with a particular reduced human chromosome complement. An increasingly large number of hybrid lines carrying rearranged chromosomes are also becoming available for regional localisation studies. We have used such hybrid cell lines to assign a gene coding for galactokinase (EC 2.7.1.6) to human chromosome 17 and to further localise the gene to band 21–22 on the long arm of the chromosome. Results from this study have provided new information Regarding the functioning of the genes coding for the Leloir pathway enzymes in man1, and have revealed a relatively close and potentially useful linkage between the genes for galactokinase and for thymidine kinase (EC 2.7.1.21).

Published
1974
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3. Assignment of human esterase-D gene to chromosome 13

Author

Frank H. Ruddle, E.A. Nichols, R.P. Creagan, and Suzie Chen

Subjects
Male, Genetics, Polymorphism, Genetic, Esterases, Chromosome Mapping, Hybrid Cells, Biology, Cell Line, Phenotype, Humans, Female, Esterase D, Molecular Biology, Gene, Chromosomes, Human, 13-15, Genetics (clinical), Chromosome 13
Published
1975
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4. Localization of the human α-globin structural gene to chromosome 16 in somatic cell hybrids by molecular hybridization assay

Author

Raju Kucherlapati, Frank H. Ruddle, Patricia Turner, Arthur W. Nienhuis, R.P. Creagan, Jeanne Catherine Lawrence, W. French Anderson, Ramon Velez, and Albert B. Deisseroth

Subjects
Genetics, Chromosome 7 (human), Genetic Linkage, Chromosome Mapping, Nucleic Acid Hybridization, DNA, Hybrid Cells, Biology, Molecular biology, General Biochemistry, Genetics and Molecular Biology, Clone Cells, Globins, Chromosome 17 (human), Chromosome 4, Chromosome 16, Genes, Chromosome 3, Chromosome 18, Humans, Chromosome 21, Chromosome 22, Chromosomes, Human, 16-18
Abstract

We have used 16 human × mouse somatic cell hybrids containing a variable number of human chromosomes to demonstrate that the human α-globin gene is on chromosome 16. Globin gene sequences were detected by annealing purified human α-globin complementary DNA to DNA extracted from hybrid cells. Human and mouse chromosomes were distinguished by Hoechst fluorescent centromeric banding, and the individual human chromosomes were identified in the same spreads by Giemsa trypsin banding. Isozyme markers for 17 different human chromosomes were also tested in the 16 clones which have been characterized. The absence of chromosomal translocation in all hybrid clones strongly positive for the α-globin gene was established by differential staining of mouse and human chromosomes with Giemsa 11 staining. The presence of human chromosomes in hybrid cell clones which were devoid of human α-globin genes served to exclude all human chromosomes except 6, 9, 14 and 16. Among the clones negative for human α-globin sequences, one contained chromosome 2 (JFA 14a 5), three contained chromosome 4 (AHA 16E, AHA 3D and WAV R4D) and two contained chromosome 5 (AHA 16E and JFA14a 13 5) in >10% of metaphase spreads. These data excluded human chromosomes 2, 4 and 5 which had been suggested by other investigators to contain human globin genes. Only chromosome 16 was present in each one of the three hybrid cell clones found to be strongly positive for the human α-globin gene. Two clones (WAIV A and WAV) positive for the human α-globin gene and chromosome 16 were counter-selected in medium which kills cells retaining chromosome 16. In each case, the resulting hybrid populations lacked both human chromosome 16 and the α-globin gene. These studies establish the localization of the human α-globin gene to chromosome 16 and represent the first assignment of a nonexpressed unique gene by direct detection of its DNA sequences in somatic cell hybrids.

Published
1977
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5. Subject Index Vol. 16, 1976

Author

D.E. Wilson, P. Shaffer-Berman, E. Solomon, N. Shimizu, S. Goss, P. DeBona, A.G. Steinberg, R.M. Siervogel, A.S. Henderson, T.-S. Chan, J.E. Anderson, A.B. Deisseroth, J.A. Anderson, D. Weil, A.D. Merritt, M. Velivasakis, D. Cox, P. Meera Khan, K.M. Overton, G.B. Côté, E. Lovrien, J.A. Brown, S. Chen, C. Finaz, R.H. Schwartz, I.W. Craig, J. Courval, B. Olaisen, P. Teisberg, J.A. Robinson, P.L. Pearson, M.C. DuVal, N. Busby, M. Ray, T.P. Webb, R.H. Kennett, C.J. Chern, P.J. Davies, I. Schreuder, B. Kaiser McCaw, H. Wyandt, J. Mohr, A. Icén, K.E. Buckton, A.R. Dunn, A.W. Nienhuis, J.V. Higgins, R.G. Worton, P.J.L. Cook, G. Spowart, J.A. Norton, J.E. Noades, R.S. Kucherlapati, W.S. Volkers, T.B. Shows, H. Wang, R.A. Raeburn, Matthew Parks, R.E. Magenis, M. Bobrow, S. Hansen, H.M. Dick, S.H. Boyer, S.H. Hsu, Veronica van Heyningen, K.R.M. Pai, R.A. Fisher, D.P. Aden, C.A. Slaughter, A.B. Bijnen, R.D. Koler, S. Guttormsen, R. Sanger, D. Linder, F. Hecht, D. Bacon, R.B. Surana, P.L. Yu, L.A. Klobutcher, O. Smithies, T.T. Puck, D.A. Hopkinson, D. Warburton, D. Shaw, K. Berg, S.J. Goss, L.L.L. Wijnen, D.A. Meyers, A. Heiberg, T. Gedde-Dahl, E. Hackel, C. Cochet, J. Frézal, C.M. Giles, A.A. Biegel, I.P. Gormley, M. Smith, F.-T. Kao, P. Vuopio, B. Schacter, B. Clark, J.D. Minna, T.A. Tedesco, M.G. Brown, C.J. Glueck, K.G. Orkwiszewski, H. Skre, K. Hirschhorn, N. Van Cong, W.F. Bodmer, W.G. Burgerhout, B.A. Doppert, W. Bodmer, G. Skude, A. Boué, E.A. Jones, K.C. Atwood, S. McDonald, C.M. Croce, E. Eicher, E.S. Seravalli, R.C.P. Go, C.G. Palmer, E.B. Robson, K. Bender, D.A. Aitken, R.P. Creagan, W.F. Anderson, P.H. Gallimore, J.B. Dossetor, W. Putt, M. Lewis, V. Lewis, F.H. Ruddle, J. Ott, H. Eiberg, M. Rivas, T.H. Marshall, M.E. Hodes, K.D. Smith, P. Bowen, A.P.M. Jongsma, R. Bigley, N. Lea, G.F. Brooks, H. Kaita, L. Wisniewski, E.R. Giblett, H.E. Faber, R.C. Elston, P. Couillin, W.B. Bias, H. Koprowski, R. Rebourcet, C.T. Falk, M.T. Yu, L.J. Stevens, U. Francke, J.J. van Rood, G. Pontecorvo, P.E. Polani, D. Patterson, E.W. Lovrien, E.A. Nichols, J.L. Hamerton, D.P. Singal, J.K. McDougall, M.L. Rivas, T. Reber, B.H. Petersen, S. Povey, Erik Thorsby, G. Khoury, E. Tolley, W.R. Breg, T. Hassold, L. Kunkel, R.L. Eddy, L.L. Haley, J. Shuster, M.A. Ferguson-Smith, D.W. Buck, R.M. Denney, K. Lange, F.H. Allen, B.R. Migeon, P. Goodfellow, R. Kucherlapati, L.R. Weitkamp, J. de Grouchy, B.M. Turner, S.S. Wachtel, T. Mohandas, P.M. Conneally, M. Siniscalco, M.D. Poulik, R. Velez, A. Hershberg, B. Moore, G.C. Koo, V.M. Chapman, A. Westerveld, B. Hellkuhl, H.P. Klinger, D. Bootsma, K.K. Namboodiri, G. Wullems, O.J. Miller, P. Gold, P. Perry, P.J. McAlpine, P.A. Lalley, A. Brøgger, J. Schlaut, M.W. Thompson, A. de la Chapelle, B. Chown, A.G. Searle, I. Pagan-Charry, C. Jones, J. van der Horst, H. van Someren, K. Willecke, E. Johnston, J.H. Edwards, W.J. Mellman, K.-H. Grzeschik, S. Ohno, P. Teesdale, C.S.N. Lee, L. Wang, and V.S. Turner

Subjects
Index (economics), Statistics, Genetics, Subject (documents), Biology, Molecular Biology, Genetics (clinical)
Published
1976
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6. Contents, Vol. 14, 1975

Author

T. Gedde-Dahl, P.M Conneally, G.R. Douglas, J. Prosser, E. Grace, J.L. Hamerton, K.-H. Grzeschik, M.A. Ferguson-Smith, P.L. Pearson, G. Corneo, E.A. Nichols, G.J. Wullems, M. Bobrow, N.C. Sun, J.E. Syrett, J.K. McDougall, R. Rebourcet, J. Frézal, V.A. McKusick, F.H. Ruddle, W. Keijzer, A. Westerveld, T.G. Gedde-Dahl, R.M. Baker, R.C. Miller, F. Hecht, P. Goodfellow, B. Olaisen, J. Ott, A.J.J. Reuser, A. Vust, C.W.H. Partridge, D.R. Bolling, J. Fleming, S.E. Gardiner, P.J. McAlpine, D.M. Steffensen, E.R. Giblett, D. Kacian, E.M. Wurzer-Figurelli, R.S. Kucherlapati, T.B. Shows, K. Berg, H. Bosker, K.C. Atwood, M.C. Rattazzi, D.W. Buck, K.W. Jones, A.G.J.M. van der Linden, R.P. Clayton, J. German, W. Prensky, V. Niewczas-Late, D. Bergsma, C.M. Giles, D.A. Meyers, E.M. Eicher, D.S. Borgaonkar, D. Mutton, K.P. Glen, P.M. Ellis, K. Hirschhorn, E. Jones, S. Povey, A. Hoogeveen, J. van der Horst, J.J. van Rood, P.M. Price, B. Bengtsson, W.G. Burgerhout, C.C. Chang, L.R. Weitkamp, R.E. Giles, E. Magenis, H. van Someren, H.M.A. Beyersbergen van Henegouwen, R.A. Buckland, K. Willecke, U. Francke, D. Bootsma, C. Partridge, R. Kennett, E. Johnston, D.A. Hopkinson, A.E. Greene, O. Mutchinick, R.P. Creagan, R.S.K. Chaganti, L. Komarnicki, T. Mohandas, M. Fellous, K. Fenger, J.H. Edwards, G. Beckman, A.S. Henderson, M.L. Rivas, L. Beckman, W.R.T. Los, L.L. Coriell, I. Purdom, N. van Cong, P. Meera Khan, H.A. de Wit-Verbeek, G.B. Côté, A.R. Mitchell, Veronica van Heyningen, S.A. Sørensen, L. Wang, E. Solomon, J.A. Brown, W.S. Volkers, H. Galjaard, J.E. Anderson, C. Pangalos, A.P.M. Jongsma, R. Sanger, S.M. Elsevier, E. Lovrien, A.B. Bijnen, M.K. Fagerhol, J.A. Pierce, M. Lewis, E.H.Y. Chu, W.B. Bias, D. Weil, S. Chen, J. de Wit, C.G. Palmer, P.W. Teesdale, F.A. McMorris, G. Miller, A.D. Merritt, R.G. Davidson, J.R. Gosden, I. Schreuder, W.F. Bodmer, P.J.L. Cook, J.E. Gray, N. Busby, H. Kaita, R.C. Elston, Y.-S. Teng, S.M. Bowser Riley, R.A. Oosterbaan, P.L. Townes, E.B. Robson, H.J. Evans, L.E. Nijenhuis, and A. Hagemeijer

Subjects
Botany, Genetics, Biology, Molecular Biology, Genetics (clinical)
Published
1975
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7. Synteny relationships of four human genes: mannose phosphate isomerase to pyruvate kinase-3 and triose phophate isomerase to lactate dehydrogenase-B

Author

Raju Kucherlapati, Frank H. Ruddle, D.S. Borgaonkar, R.P. Creagan, and E.A. Nichols

Subjects
Pyruvate decarboxylation, Pyruvate dehydrogenase kinase, Pyruvate Kinase, Isomerase, Hybrid Cells, Biology, Pyruvate dehydrogenase phosphatase, Cell Line, Triosephosphate isomerase, Cricetinae, Genetics, Animals, Humans, Molecular Biology, Genetics (clinical), Recombination, Genetic, L-Lactate Dehydrogenase, Mannose phosphate isomerase, Chromosome Mapping, Pyruvate dehydrogenase complex, Molecular biology, Genes, Biochemistry, Carbohydrate Epimerases, Pyruvate kinase, Triose-Phosphate Isomerase
Published
1975
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8. References to the committee reports

Author

L.R. Weitkamp, J.E. Anderson, F.A. McMorris, L. Komarnicki, O. Mutchinick, S.M. Elsevier, T. Mohandas, J.L. Hamerton, G. Miller, J.R. Gosden, E. Lovrien, D.R. Bolling, J. Fleming, H.J. Evans, G.J. Wullems, J. Prosser, N.C. Sun, H. Bosker, K.C. Atwood, W. Keijzer, H.M.A. Beyersbergen van Henegouwen, A.B. Bijnen, M.K. Fagerhol, J. Ott, J. van der Horst, E. Grace, C.W.H. Partridge, R.C. Miller, Y.-S. Teng, P.J. McAlpine, J.E. Gray, J. de Wit, H. van Someren, K. Willecke, K. Hirschhorn, V. Niewczas-Late, T.B. Shows, D.A. Hopkinson, A.E. Greene, L. Beckman, J.A. Pierce, H.A. de Wit-Verbeek, P.W. Teesdale, R. Kennett, E. Johnston, R.E. Giles, G.B. Côté, P.L. Pearson, E. Magenis, A.G.J.M. van der Linden, M. Bobrow, V.A. McKusick, N. van Cong, J.H. Edwards, A.D. Merritt, P. Meera Khan, R.G. Davidson, P.L. Townes, I. Schreuder, S.A. Sørensen, K.W. Jones, J. Frézal, M.L. Rivas, E.B. Robson, D. Weil, A. Hoogeveen, R.A. Buckland, J.J. van Rood, K.-H. Grzeschik, E. Jones, E.A. Nichols, W.F. Bodmer, P.J.L. Cook, D.S. Borgaonkar, L. Wang, E. Solomon, B. Olaisen, K. Fenger, A.S. Henderson, A.J.J. Reuser, A. Vust, G. Beckman, E.M. Wurzer-Figurelli, J.E. Syrett, W.G. Burgerhout, C.C. Chang, D. Kacian, Veronica van Heyningen, S.E. Gardiner, A.R. Mitchell, W.B. Bias, C.M. Giles, S. Chen, F. Hecht, J.A. Brown, N. Busby, H. Kaita, C.G. Palmer, D.A. Meyers, R.C. Elston, T.G. Gedde-Dahl, R.M. Baker, A.P.M. Jongsma, K. Berg, R.S. Kucherlapati, L.E. Nijenhuis, I. Purdom, R. Sanger, R.P. Clayton, U. Francke, J.K. McDougall, J. German, P.M. Ellis, S.M. Bowser Riley, P.M. Price, M. Lewis, S. Povey, E.H.Y. Chu, R.A. Oosterbaan, L.L. Coriell, P. Goodfellow, D. Mutton, K.P. Glen, C. Partridge, R.S.K. Chaganti, D. Bootsma, M. Fellous, W. Prensky, W.R.T. Los, D.M. Steffensen, G. Corneo, D.W. Buck, E.M. Eicher, A. Westerveld, B. Bengtsson, M.C. Rattazzi, F.H. Ruddle, E.R. Giblett, W.S. Volkers, R.P. Creagan, T. Gedde-Dahl, P.M Conneally, G.R. Douglas, M.A. Ferguson-Smith, H. Galjaard, C. Pangalos, R. Rebourcet, D. Bergsma, and A. Hagemeijer

Subjects
Genetics, Library science, Biology, Bioinformatics, Molecular Biology, Genetics (clinical)
Published
1975
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9. Assignment of the genes for human peptidase A to chromosome 18 and cytoplasmic glutamic oxaloacetate transaminase to chromosome 10 using somatic-cell hybrids

Author

F. Ricciuti, M. Hirschi, Suzie Chen, T.R. Chen, Frank H. Ruddle, R.P. Creagan, Jay A. Tischfield, and F.A. McMorris

Subjects
Peptide Biosynthesis, Cytoplasm, Mitosis, Transaminase, Mice, Chromosome 18, Genetics, Animals, Humans, Citrate synthase, Molecular Biology, Gene, Transaminases, Genetics (clinical), Chromosomes, Human, 16-18, Chromosomes, Human, 6-12 and X, biology, Chromosome Mapping, Chromosome, Karyotype, Genes, Biochemistry, Karyotyping, biology.protein, Peptides
Abstract

Evidence based on analysis of mouse-human somatic-cell hybrids is presented that supports the assignment for the structural loci of peptidase A to human chromosome 18 and of cytoplasmic glutamate oxaloacetate transaminase (GOT) (EC 2.6.1.1) to chromosome 10. The medical and evolutionary significance of these assignments is discussed.

Published
1973
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11. Assignment of a Gene for Adenosine Deaminase to Human Chromosome 20

Author

Frank H. Ruddle, Jay A. Tischfield, R.P. Creagan, and E.A. Nichols

Subjects
Adenosine, Erythrocytes, Genetic Linkage, Somatic cell, Chromosomes, Human, 19-20, Electrophoresis, Starch Gel, Hybrid Cells, Somatic Cell Hybridization, Isozyme, Mice, Adenosine deaminase, Aminohydrolases, Genetics, Animals, Humans, Gene, Genetics (clinical), Staining and Labeling, biology, Chromosome Mapping, Molecular biology, Clone Cells, Isoenzymes, Biochemistry, Karyotyping, biology.protein, Chromosome 20, Combined immunodeficiency syndrome
Abstract

Evidence, based on five series of mouse-human somatic cell hybrid clones, is presented for assignment of a human gene for adenosine deaminase (ADA: EC 3.5.4.4) to chromosome 20. The human form of adenosine deaminase expressed by the hybrid cells apeared to be identical to the isozyme found in erythrocytes, judging from its electrophoretic mobility and reactions with p-chloromercuribenzoic acid (PCMB). Absence of ADA activity has been associated with a combined immunodeficiency syndrome. The relationship of our data to hypotheses concerning this association is discussed.

Published
1974
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14. Confirmation of the Synteny of the Human Genes for Cytoplasmic Isocitrate Dehydrogenase and Cytoplasmic Malate Dehydrogenase and Assignment to Chromosome 2

Author

Raju Kucherlapati, Frank H. Ruddle, R.P. Creagan, Y. H. Tan, B. Carritt, Suzie Chen, F.A. McMorris, F. Ricciuti, and Jay A. Tischfield

Subjects
Cytoplasm, Chromosomes, Human, 1-3, Chromosome Mapping, Chromosome, Hybrid Cells, Biology, Molecular biology, Malate dehydrogenase, Isocitrate Dehydrogenase, Clone Cells, Mice, Isocitrate dehydrogenase, Biochemistry, Malate Dehydrogenase, Genetics, Animals, Humans, Human genome, Molecular Biology, Genetics (clinical), Synteny
Published
1974
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15. Standard karyotype of the Norway rat, Rattus norvegicus

Author

Karen Meilinger, Kamlesh Madan, S. Pathak, J.V. Neel, Martin Bobrow, Frank H. Ruddle, Jay A. Tischfield, R.P. Creagan, Suzie Chen, F.A. McMorris, T.R. Chen, M. Hirschi, T. Tsuchimoto, F. Ricciuti, T.C. Hsu, D.M. Cox, A.D. Bloom, and T. Utakoji

Subjects
Genetics, Zoology, Karyotype, Biology, Molecular Biology, Genetics (clinical)
Published
1973
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16. Chromosome Assignments in Man of the Genes for Two Hexosephosphate Isomerases

Author

F. Ricciuti, Frank H. Ruddle, T.R. Chen, R.P. Creagan, Jay A. Tischfield, and F.A. McMorris

Subjects
Chromosomes, Human, 19-20, Electrophoresis, Starch Gel, Isomerase, Hybrid Cells, Biology, Chromosomes, Mice, Neuroblastoma, Chromosome 15, L Cells, Glucosephosphate Isomerase, Leukocytes, Animals, Humans, Gene, chemistry.chemical_classification, Genetics, Multidisciplinary, Staining and Labeling, Somatic Cell Hybrids, Chromosomes, Human, 1-3, Glucose-6-Phosphate Isomerase, Chromosome, Fibroblasts, Parainfluenza Virus 1, Human, Enzyme, Genes, chemistry, Electrophoresis, Polyacrylamide Gel, Carbohydrate Epimerases, Oxidoreductases, Mannose, Chromosome 22, Peptide Hydrolases
Abstract

Thirty-seven clones of somatic cell hybrids between human and mouse cells were examined for retention of human chromosomes and expression of human constitutive enzymes. Human glucosephosphate isomerase and chromosome F-19 were retained or lost concordantly, as were human mannosephosphate isomerase and chromosome C-7. The genes for the enzymes are thus assigned to these two chromosomes.

Published
1973
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18. Mouse/Human Somatic Cell Hybrids Utilizing Human Parental Cells Containing a (14;22) Translocation: Assignment of the Gene for Nucleoside Phosphorylase to Chromosome 14

Author

Suzie Chen, Y. H. Tan, Frank H. Ruddle, R.P. Creagan, and Jay A. Tischfield

Subjects
Chromosome Aberrations, Genetics, Somatic cell, Chromosome Mapping, Chromosome, Purine nucleoside phosphorylase, Chromosomal translocation, Hybrid Cells, Biology, Molecular biology, Clone Cells, Mice, Somatic fusion, Chromosomes, Human, 21-22 and Y, Animals, Humans, Pentosyltransferases, Molecular Biology, Gene, Chromosomes, Human, 13-15, Genetics (clinical), Hybrid
Published
1974
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22. Genetic homology between man and the chimpanzee: syntenic relationships of genes for galactokinase and thymidine kinase and adenovirus-12-induced gaps using chimpanzee-mouse somatic cell hybrids

Author

Valerie Lewis, J.K. McDougall, Frank H. Ruddle, Suzie Chen, and R.P. Creagan

Subjects
Pan troglodytes, Biology, Hybrid Cells, medicine.disease_cause, Thymidine Kinase, Homology (biology), Chromosomes, Adenoviridae, Mice, Species Specificity, Genetics, medicine, Animals, Humans, Gene, Synteny, Chromosomal inversion, Chromosome Aberrations, Phosphotransferases, Chromosome Mapping, General Medicine, Galactokinase, Molecular biology, Chromosome 17 (human), Genes, Thymidine kinase
Abstract

The induction by adenovirus-12 of a site-specific gap and assignment of the chimpanzee genes for thymidine kinase and galactokinase were studied by utilizing chimpanzee-mouse hybrid cells. It has been shown that adenovirus-12 induces a specific gap in the long arm of human chromosome 17 (HS 17); with chimpanzee-mouse hybrid cells the specific gap appears on the short arm of the chimpanzee homolog [PTR 19 (HS 17)] of HS 17. This result supports the proposed relationship of HS 17 to PTR 19 (HS 17) by means of a pericentric inversion. The chimpanzee thymidine kinase and galactokinase genes were assigned to PTR 19 (HS 17), further confirming the homology to HS 17. Other syntenic relationships and gene assignments were consistent with proposed homologies between chimpanzee and human chromosomes.

Published
1976

23. Assignment and regional localization of a gene coding for galactokinase to human chromosome 17q21-22

Author

K. Willecke, E.A. Nichols, J.K. McDougall, S.M. Elsevier, Richard E. Giles, Raju Kucherlapati, Frank H. Ruddle, and R.P. Creagan

Subjects
Genetics, Phosphotransferases, Chromosome Mapping, Galactose, Biology, Hybrid Cells, Galactokinase, Cell Line, Mice, Chromosome (genetic algorithm), Chromosomes, Human, 21-22 and Y, Animals, Humans, Molecular Biology, Gene, Genetics (clinical), Coding (social sciences), Chromosomes, Human, 16-18
Published
1975

24. Genetic analysis of the cell surface: association of human chromosome 5 with sensitivity to diphtheria toxin in mouse-human somatic cell hybrids

Author

Suzie Chen, Frank H. Ruddle, and R.P. Creagan

Subjects
Somatic cell, Cell, Biology, Hybrid Cells, medicine.disease_cause, L Cells (Cell Line), Cell membrane, Mice, L Cells, Leucine, medicine, Animals, Humans, Diphtheria Toxin, Chromosomes, Human, 4-5, Lung, Diphtheria toxin, Multidisciplinary, Toxin, Fibroblasts, Molecular biology, Cell biology, Clone Cells, Elongation factor, medicine.anatomical_structure, ADP-ribosylation, Karyotyping, Protein Biosynthesis, Research Article, HeLa Cells
Abstract

Diphtheria toxin inhibits protein synthesis in eukaryotic cells by catalyzing inactivation of elongation factor 2. The 10,000-fold greater sensitivity in vitro to diphtheria toxin of human cells as compared to mouse cells seems to be attributable to a difference at the level of the cell membrane. Mouse-human cell hybrids are as sensitive to diphtheria toxin as human cells. We have shown that the sensitivity of the hybrid cells is due to a gene or genes located on human chromosome 5. Mouse-human hybrid cells in which chromosome 5 is present are as sensitive to the toxin as human cells, which hybrids without chromosome 5 are as resistant as mouse cells. Entry of toxin into cells seems to be a two-step process involvin, (1) binding of toxin to the cell surface and (2) endocytotic uptake of toxin. The difference in sensitivity between human and mouse cells and between hybrid cells with and without chromosome 5 does not appear to be due to a difference in endocytotic activity and may be due to presence or absence of toxin-specific receptor.

Published
1975

25. Mapping of genes and adenovirus-12-induced gaps using chimpanzee-mouse somatic cell hybrids

Author

J. K. McDougall, V. Lewis, Frank H. Ruddle, R.P. Creagan, and Suzie Chen

Subjects
Genetics, Cell fusion, Pan troglodytes, Somatic Cell Hybrids, Chromosome Mapping, Biology, Hybrid Cells, medicine.disease_cause, Adenoviridae, Cell Line, Cell Fusion, Mice, Genes, Cell culture, medicine, Animals, Molecular Biology, Gene, Genetics (clinical)
Published
1976

27. Chromosome assignments of genes in man using mouse-human somatic cell hybrids: mitochondrial superoxide dismutase (indophenol oxidase-B, tetrameric) to chromosome 6

Author

Frank H. Ruddle, R.P. Creagan, Jay A. Tischfield, and F. Ricciuti

Subjects
Somatic cell, Malic enzyme, Chromosomes, Cell Line, Superoxide dismutase, Electron Transport Complex IV, chemistry.chemical_compound, Mice, Malate Dehydrogenase, Genetics, Animals, Humans, Gene, Genetics (clinical), Synteny, Chromosomes, Human, 6-12 and X, biology, Superoxide, Superoxide Dismutase, Chromosome, Chromosome Mapping, Molecular biology, Clone Cells, Mitochondria, Biochemistry, chemistry, Cytoplasm, Genetic Code, biology.protein, Hybridization, Genetic
Abstract

Evidence from mouse/human somatic cell hybrids is presented for the synteny of the genes for indophenol oxidase-B (tetrameric) and cytoplasmic malic enzyme (EC 1.1.1.40). Assignment of these two genes to chromosome 6 is further confirmed. The identification of indophenol oxidase-B (tetrameric) as mitochondrial superoxide dismutase is discussed.

Published
1973

28. Proceedings: Assignment of the gene in man for mitochondrial superoxide dismutase (indophenol oxidase-B, tetrameric) to chromosome 6

Author

F. Ricciuti, Frank H. Ruddle, R.P. Creagan, and Jay A. Tischfield

Subjects
Male, Mitochondrion, Hybrid Cells, Malate dehydrogenase, Superoxide dismutase, Electron Transport Complex IV, Mice, Mitochondrial Superoxide Dismutase, Malate Dehydrogenase, Genetics, Animals, Humans, Molecular Biology, Gene, Genetics (clinical), Chromosomes, Human, 6-12 and X, biology, Superoxide Dismutase, Chromosome, Chromosome Mapping, INDOPHENOL OXIDASE B, Molecular biology, Clone Cells, Mitochondria, biology.protein
Published
1974

29. Somatic cell genetic assignment of peptidase C and the Rh linkage group to chromosome A-1 in man

Author

F.A. McMorris, Jay A. Tischfield, Frank H. Ruddle, F. Ricciuti, R.P. Creagan, Teh Kuei Chen, and Gretchen J. Darlington

Subjects
Somatic cell, Genetic Linkage, G banding, Biology, Hybrid Cells, Mice, Quinacrine Mustard, Constitutive heterochromatin, Animals, Humans, Synteny, Genetics, Multidisciplinary, Rh-Hr Blood-Group System, Staining and Labeling, Chromosomes, Human, 1-3, Chromosome, Chromosome Mapping, Molecular biology, Phenotype, Clone Cells, Genes, Quinacrine, Karyotyping, Rh blood group system, Peptide Hydrolases
Abstract

The segregation of the human peptidase-C phenotype in five different series of human-mouse hybrid clones was examined. The chromosome constitution of these hybrids was determined by quinacrine mustard fluorescence, Giemsa banding, and constitutive heterochromatin staining. That the clones could be classified without exception either as human peptidase C positive/ A-1 positive (14 clones), or as peptidase C negative/ A-1 negative (12 clones) indicates that peptidase C can be assigned to the human A-i chromosome. Data from an extensive series of human-mouse clones used provide support for the syntenic association between peptidase C and phosphoglucomutase-1 and by inference a linkage of both to Rh factor group.

Published
1972

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