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Introduction: Municipal solid waste (MSW) incineration fly ash is a harmful residue formed during the incineration process. It contains high concentrations of hazardous heavy metals, such as lead, zinc, aluminum, and iron.Methodology: In this study, bioleaching integrated with an electrokinetic approach for heavy metal remediation from MSW incineration fly ash using Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans bacteria was tested.Results and discussion: The physicochemical properties of fly ash included a particle size of 26.1 μm, with the presence of heavy metals. A. ferrooxidans and A. thiooxidans produced sulphuric acid (0.0289 M and 0.0352 M) during the proliferation; this acid enhances the bioleaching of heavy metals from fly ash. The results of an integrated approach showed an 85%, 47%, 92%, 85%, 46%, 67% 11%, and 55% removal of the heavy metals K, Na, Ca, Mg, Al, Zn, Pb, and Mg, respectively, in the presence of A. ferrooxidans. Overall, these results evidenced that heavy metals were completely removed from the fly ash using an integrated approach. Therefore, this integrated approach can be used as an effective heavy metal removal method for treating fly ash in MSW.

Objectives: The current study looks towards reporting the chemical compounds present in the panchagavya (PG), free radicals scavenging and mosquitocidal activity of PG in the laboratory condition. Methods: The existence of chemical compounds in the PG were studied by GC–MS analysis. Free radicals scavenging activity of PG was studied by using various invitro assays. Mosquitocidal efficacy of PG was studied by the experiment on larvicidal, pupicidal, adulticidal, fecundity, longevity, and ovicidal activity against An. stephensi, Ae. aegypti and Cx. quinquefasciatus. Results: GC–MS analysis revealed fifteen chemical compounds present in the PG. Free radical scavenging was done by 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), hydroxyl, and superoxide assays, and the IC50 was calculated as 37, 37.5, 35, and 38 μg/mL respectively. PG exhibited better larvae and pupae mortality against I-IV instar of Cx. quinquefasciatus (LC50: 148.765, 162.534, 187.619, 210.835 and 234.624 ppm, LC90: 286.636, 306.390, 350.276, 390.735 and 419.195 ppm). The highest adult mortality was found against An. stephensi (91.10 ± 1.74%) with the IC50 and IC90 values of 128.114 and 260.609 ppm. An. stephensi showed highly decreased fecundity and longevity even at a low concentration of PG. Inhibition of 100% egg hatchability of An. stephensi was obtained at 250 ppm followed by Ae. aegypti, and Cx. quinquefasciatus at 300 ppm respectively. On comparing with other mosquito vectors An. stephensi was effectively inhibited by PG at each stage of their life cycle. Conclusion: The results provide the first proof that PG could be a successful natural agent for controlling different mosquito vectors. Furthermore, our findings pave the way for more research into the efficacy of natural materials' mosquitocidal activities.

Background: Uranium is a toxic heavy metal, naturally present in soil and water, that causes several health effects due to free radical generation and damage to DNA. Gynmenma sylvestre is a pharmacologically important medicinal plant that has been used in the treatment of diabetes and several other ailments. Accordingly, in this analysis, we intended to examine the protective effect of G. sylvestre leaf extract (GSE) against uranium toxicity. Methods: Aqueous leaf extract of Gymnema sylvestre was prepared and its qualitative and quantitative phytochemical analysis was carried out. The antioxidant potential of the extract was analysed. Human lymphocytes isolated from freshly collected blood samples were treated with uranyl nitrate and GSE independently and in combination. The toxic effects of uranium and the protective effect of the leaf extract have been analyzed by MTT assay, micronucleus and γH2AX DNA damage response detection. Results: Phytochemical analysis revealed the presence of major and important phytochemical groups such as alkaloids, flavonoids, saponins, tannins, etc. 15 μg/mL of GSE significantly reduced toxicity in the 3 different uranium concentrations (0.25, 0.50 & 0.75 mM). Cell viability percentage increased from 90.44 to 94%, 80.40 to 86% and 72.03 to 77.40% in the GSE and uranium co-exposure groups. GSE treatment led to statistically significant reduction in the average number of micronuclei observed per 1000 binucleate cells in all three groups with values 30.66, 40 and 48.33 micronuclei respectively. GSE was successful in reducing the γH2AX percentages in the treated cell groups with statistically significant protection with values 22.11 ± 1.56, 48.43 ± 0.83 and 51.14 ± 1.78 respectively. Conclusion: This study is the first to report the in vitro toxic effects of uranium in human peripheral blood mononuclear cells as well as the protective effect of Gymnema sylvestre against uranium induced damages. GSE could alleviate DNA damage in cells treated with uranium in a dose-dependent way. Together these findings emphasize that GSE could be a promising protective agent against uranium-induced damages and should be further evaluated for the identification of active principle(s).

Cancer is the second largest killer in the world. Even though the treatment and diagnosis of cancers have increased manifold, the survival rate has not increased proportionately. This discrepancy is mainly due to the toxicity of the chemotherapeutic agents. Natural herbs possessing significant antitumour activity can be utilized to address this issue. Asparagus racemosus is a widely distributed medicinal plant having reported antitumor properties. A concomitant use of the aqueous extract of Asparagus racemous (AEAR) along with Cyclophosphamide (CTX) was studied for the reversal of myelosuppression and hemotoxicity induced by CTX and to test whether AEAR possess significant antitumor activity against Dalton’s Lymphoma Ascites (DLA) tumour in BALB/c mice model. The study consisted of preliminary physico-chemical character determination and phytochemical screening. AEAR was used at 216, 432 and 864 g/kg body weight doses designated as lower, medium and high doses respectively and CTX was used at 25 mg/kg body weight. Hemogram, total leucocyte count, differential leucocyte count, bone marrow cellularity and – esterase positive cells were assessed. Malondialdehyde (MDA), glutathione, superoxide dismutase (SOD) and catalase (CAT) levels were estimated as oxidative stress markers. Tumour volume, size, mean survival time, increase in life span and in vitro cytotoxicity assays involving trypan blue exclusion test and MTT assay were done for antitumor study. AEAR showed significant elevation in hemogram, total leucocyte count, differential count, bone marrow cellularity and -esterase positive cell activity. AEAR at medium and high dose levels showed significant elevation in anti-oxidant enzyme levels of MDA, Glutathione, SOD and CAT enzymes. They also reduced the tumour volume, size and significantly increased the life span and mean survival time of DLA bearing mice. In in vitro cytotoxicity tests also, the medium and high dose of AEAR showed significant cytotoxicity. AEAR at doses of 432 and 864 mg/kg body weight doses significantly reverses the myelosuppression and hemotoxicity. It enhances the levels of antioxidant enzymes and is also an antitumor agent and exerts significant cytotoxicity against DLA tumour cells. Asparagus racemosus provides a ray of hope in addressing the toxicity while using chemotherapeutic agents when used as an adjuvant therapy.

Search for medicinal plants to treat liver disorders is an important research topic on herbs. Acute toxicity study is a prerequisite for safety and dose fixation for further pharmacological actions. In the present study, aqueous and 95% ethanolic extract of whole plant of Helicanthus elastica were subjected to acute oral toxicity. The aqueous and ethanolic extract revealed no observable changes in the rats up to the dose level of 2,000 mg /kg body weight. The extracts were then screened for paracetamol-induced hepatic injury at dose levels of 200 and 400 mg/kg body weight (1/10 and 1/5 LD50 based on toxicity study). The aqueous extract of whole plant of H. elastica was found to produce significant (p

Foodborne pathogens continue to challenge public health due to their ability to cause severe illness and their increasing resistance to current antimicrobial treatments. Listeria monocytogenes is a resilient foodborne pathogen that poses significant risks to vulnerable populations, leading to severe infections and high hospitalization rates. The emergence of antimicrobial-resistant (AMR) strains of L. monocytogenes underscores the need for novel therapeutic strategies. In this study, we investigated the antimicrobial efficacy of the (2E)-3-(3,5-dibromo-2-hydroxylphenyl)-1-(5-methylfuran-2-yl) prop-2-en-1-one (DK06) against multidrug-resistant L. monocytogenes. DK06 exhibited a significant dose-dependent inhibition of L. monocytogenes growth, achieving a maximum inhibition of 92.9 % at 320 μM. Molecular docking and dynamics simulations revealed high binding affinities for key virulence proteins PlcB and ArgA, with stable protein-ligand interactions. DK06 also disrupted biofilm formation at sub-MIC levels, reducing extracellular polymeric substances (EPS) and biofilm mass, as observed by scanning electron microscopy (SEM) analysis. Furthermore, DK06 downregulated the expression of virulence genes (plcB, argA, and hly) and decreased hemolytic activity. In vivo zebrafish studies confirmed the safety of DK06 up to 80 μM, demonstrating its efficacy in reducing mortality and oxidative stress associated with L. monocytogenes infection. DK06 also attenuated inflammation by downregulating key inflammatory markers (tnfa, il1b, il6, and nfkb). These findings indicate that DK06 is a promising multi-target inhibitor with potential application in treating infections and combating antimicrobial resistance., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Azo dyes are the most varied class of synthetic chemicals with non-degradable characteristics. They are complex compounds made up of many different parts. It was primarily utilized for various application procedures in the dyeing industry. Therefore, it's crucial to develop an economical and environmentally friendly approach to treating azo dyes. Our present investigation is an integrated approach to the electrooxidation (EO) process of azo dyes using RuO 2 -IrO 2 -TiO 2 (anode) and titanium mesh (cathode) electrodes, followed by the biodegradation process (BD) of the treated EO dyes. Chemical oxygen demand (COD) removal efficiency as follows MB (55%) ≥ MR (45%) ≥ TB (38%) ≥ CR (37%) correspondingly. The fragment generated during the degradation process which was identified with high-resolution mass spectrometry (HRMS) and its degradation mechanism pathway was proposed as demethylation reaction and N-N and C-N/C-S cleavage reaction occurs during EO. In biodegradation studies by Aeromonas hydrophila AR1, the EO treated dyes were completely mineralized aerobically which was evident by the COD removal efficiency as MB (98%) ≥ MR (92.9%) ≥ TB (88%) ≥ CR (87%) respectively. The EO process of dyes produced intermediate components with lower molecular weights, which was effectively utilized by the Aeromonas hydrophila AR1 and resulted in higher degradation efficiency 98%. We reported the significance of the enhanced approach of electrochemical oxidation with biodegradation studies in the effective removal of the pollutants in dye industrial effluent contaminated water environment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

A new species, Bathysphyraenops radhae, is described from the equatorial western Indian Ocean. It resembles Bathysphyraenops declivifrons in the shape of snout and number of pseudobranchial filaments in adults but can be readily distinguished from all known members of Howellidae by its characteristically armed preopercle and in strong reduction or absence of spinules on scales around the pectoral fin. The genus Bathysphyraenops is rediagnosed, and the characteristics useful in diagnosing the howellid genera are briefly discussed. The nominal genus Schistoperca is synonymized with Bathysphyraenops. A key to the species of Bathysphyraenops is provided., (© 2022 Fisheries Society of the British Isles.)

Recently, researchers are concentrating on the synthesis of composite materials to enhance the efficiency of the materials in various applications. In this work, nickel vanadium oxide (NiV 2 O 6 ) nanocomposite material is prepared via two methods and the prepared samples have been characterized with basic studies to analyse the effect of preparation method and the reaction time. The XRD studies reveal a polycrystalline growth in both the methods. The broad XRD peaks obtained for samples prepared via hydrothermal method suggests the size reduction and 1D nanostructure formation. The SEM analysis shows the formation of 1D structures in hydrothermal and 3D microsphere structures in solvothermal methods. The possible formation mechanism behind this formation has been discussed in this manuscript. The FTIR peaks in the fingerprint region confirm the formation and vibration of metal-oxygen bonds. The large optical bandgap values obtained from Tauc plot again confirms the formation of nanostructures of the synthesized samples. The photocatalytic activity of nickel vanadium oxide on methylene blue dye under halogen light were performed and, the recyclability of the sample is investigated. It was found from the photocatalytic spectrum that, the samples prepared from both the methods shows a degradation efficiency of more than 80% within 150 min. It was confirmed that the prepared NiV 2 O 6 photocatalyst samples does not lose their degradation ability even after five cycles of repeated usage., (Copyright © 2022 Elsevier Inc. All rights reserved.)

Peptide-based drug development is an emerging and promising approach in cancer therapeutics. The present study focuses on understanding the mechanism of MP12 peptide (MDNHVCIPLCPP) derived from cysteine-rich trypsin inhibitor protein of virulence factor of pathogenic fungus Aphanomyces invadans. MP12 is involved in antiproliferative activity against the human laryngeal epithelial cell (Hep-2), demonstrated in this study. MP12 sequence showed a significant binding score and has multiple hydrogen bond interactions with the proteins that play a vital role in apoptotic pathways such as Bcl-2, caspase-3, caspase-7, and XIAP. Based on the bioinformatics characterization and molecular docking result, further study was focused on MP12 antiproliferative activity. The peptide showed a dose-dependent inhibition against Hep-2 cell line proliferation, analyzed over MTT and neutral red uptake assays. The IC 50 value of the MP12 peptide was calculated based on the antiproliferative property (24.7 ± 0.34 μM). MP12 treated Hep-2 cells showed significant shrinkage in cell morphology compared to untreated cells, inhibiting the cell cycle. The gene expression analysis validated that the MP12 significantly upregulates the caspase-3, caspase-7, and caspase-9 genes. The developmental toxicity study using zebrafish embryos as in vivo model proved that the MP12 is nontoxic. Based on the obtained results, we proposed that the peptide MP12 derived from cysteine-rich trypsin inhibitor protein of virulence molecule of pathogenic fungus have a potential antiproliferative activity. However, further clinical trials need to be focused on the mechanism and therapeutic application against laryngeal cancer., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The phenomenal increase in the demand of herbal drugs, leads to over exploitation of medicinal plants which ultimately resulted in the scarcity and endangerment of many valuable plant species. On observing the difficulties in procuring genuine herbal drugs arose the concept of substitution which was documented in many classical Ayurvedic texts. The present study made a comparative evaluation of the gastroprotective potential of hydroalcoholic extracts of an original drug Aconitum heterophyllum (HAAH) and its substitute Cyperus rotundus (HACR) in the treatment of gastric ulcer under in vivo experimental conditions. The anti-ulcer property of the plant extracts was investigated against pylorus ligation induced ulcer in Wistar albino rats. The results confirmed that both A. heterophyllum and C. rotundus deliver comparable significant protection against gastric ulcer, indicated by a decrease in the free and total acidity, volume of gastric content, total proteins and increase in pH of gastric content, total carbohydrates and total carbohydrates to total proteins ratio. The observed anti-ulcer potential of both the drugs is attributed mainly to prevention of the generation of damaging free radical cascades and oxidant radical release., (© 2019 The Authors.)

Pedalium murex L. is a medicinal herb that has been used for the treatment of diseases related to kidney in the traditional system of medicine. The current study aims to study the effect of ethyl acetate extract of P. murex (EAEP) and its fractionated compound pedalitin against urease production and UreC gene expression in Proteus mirabilis . The selected reference strain Proteus mirabilis (MTCC 425) and the isolates culture of Proteus mirabilis were subjected to study the antibacterial efficacy of P. murex . Expression analysis of P. mirabilis urease gene was successfully done by QPCR. The ethyl acetate extract effectively inhibit the reference Proteus mirabilis and bacterial isolates of Proteus mirabilis in the clinical samples studied . EAEP has showed more potent activity (56.7%) against urease enzyme and pedalitin also exhibited potent activity (30.1%). Using qPCR, the expression of UreC gene of P. mirabilis was controlled by EAEP and also its bioactive compound pedalitin. The present study clearly demonstrated the potency of P. murex in controlling the growth of pathogenic P. mirabilis and to control the expression of urease enzyme production as well as to restrict the urease gene expression in P. mirabilis ., (© 2020 The Authors.)

The wild Prunus korshinskyi has a restricted distribution in small scattered areas of Jabal Al-Lauz (2580 m above sea level), a mountain in northwestern Saudi Arabia. Major objective of current study is to provide information about P. korshinskyi by analyzing its ecology in terms of floristic diversity, plant associations, environmental features, and its size structure and nutritional value. For this purpose, 12 stands of 20 m 2 were selected along the Jabal Al-Lauz mountain ridge. Three plant associations and their environmental variables were identified and characterized after application of a two-way indicator species analysis (TWINSPAN), detrended correspondence analysis (DCA). And canonical correspondence analysis (CCA) as follows: VGI: P. korshinskyi - Astracantha echinus , VGII: Artemisia siberi-P. korshinskyi , and VGIII: Retama raetam - Artemisia sieberi . The edaphic factor affecting the distribution of the associations were pH, potassium and manganese content. The size class frequency distribution of P. korshinskyi shows a J-shape in spring and fall for the whole population; there was no regeneration via seed. Nutritional evaluation showed the fruits had a higher content of total carbohydrate, fats, crude protein, phosphorus, and potassium, and lower content of ash, Fe, Ca, Mn, Mg, and Zn than that in stems and leaves. This ecological knowledge gained through this study would be beneficial for managing and conserving of P. korshinskyi in a Jabal Al-Lauz area with its distinct and unique vegetation., Competing Interests: The authors declared that there is no conflict of interest., (© 2019 Production and hosting by Elsevier B.V. on behalf of King Saud University.)

Plants in arid regions are exposed to various abiotic stresses and the presence of the waxy cuticular layer acts as a defensive barrier, which consists mainly of long chain fatty acids, hydrocarbons and other derived compounds. Studies on the chemical composition and properties of cuticles of arid plants are scanty. The present study deals with the analysis of cuticular wax composition and effect of temperature on some ecophysiological parameters of an important arid plant Ziziphus nummularia . A total of 59 different wax compounds were detected from the leaf cuticle by capillary GC-MS. 4-Hydroxycyclohexanone, Heptacosane and 2,7-Dimethyloctane-3,5-dione were the dominant wax compounds in Z. nummularia . The variation of photosynthetic rate varied from 0.70 to 7.70 µmol CO2 m-2s-1 against the studied temperature range of 15-55 °C. The transpiration rate varies from 1.80 to 8.40 mmol H2O m-2s-1 within the temperature range of 15-55 °C. The quantum yield of photosystem II (Fv/Fm) also exhibited much variation due to the variation of temperature. The results clearly shows that Z. nummularia is highly adapted to restrict water loss and can tolerate high temperatures and can be considered as an appropriate species for vegetating the arid areas., (© 2019 The Author(s).)

Prunus arabica (Oliv.) Meikle is an endangered shrub with a restricted distribution, which inhabits banks of flood flow channels in small sites of ancient volcanic activity dominated by black basaltic boulders within the Harrat Al-Harrah protectorate, Northern Saudi Arabia. It is with limited information about its ecology and hence, the present study is focusing on its phytosociological behavior and ecological features. The multi-methodological approach comprised species description, vegetation sampling and soil composition of 9 study sites at Harrat Al-Harrah protectorate. The application of TWINSPAN, DCA and CCA multivariate analyses led to identify 3 vegetation groups (VGI: Pulicaria undulata - Prunus arabica, VGII: Prunus arabica-Artemisia seiberi , VGIII: Artemisia seiberi-Achillea fragrantissima ) associated with the distribution of P. arabica . The environmental variables that affect P. arabica and its main ecological features had been estimated and discussed. The present study concludes that the main reasons for its threatened and endangerment are seemingly due to overgrazing, poor rainfall, drought, shifting and destruction of its suitable habitat area.

Coronary Artery Disease (CAD) is a major killer disease throughout the world. Dyslipidemia is a major contributor to the risk of CAD. Several dietary articles traditionally used in India and other South Asian countries reduced dyslipidemia. The present study was undertaken to evaluate the combined effect of Mung bean ( Vigna radiata ) and Elephant foot yam ( Amorphophallus paeoniifolius ) on serum lipids and atherogenic indices in albino rats and to compare it with a standard drug Cholestyramine. Thirty healthy albino rats of both sexes (150-200 g) were randomized to 5 groups of 6 animals each. The grouping were done based on the following criteria: Group I: Normal Control Group, Group II: (Standard Group): Cholestyramine resin 5 mg/kg bw, Group III: (Half Dose Group): Drug powder at 540 mg/kg bw, Group IV: (Effective Dose Group): Drug powder at 1080 mg/kg bw, and Group V: (Double Dose Group): Drug powder at 2160 mg/kg bw. Lipid profile was estimated at the beginning and after 30 days of treatment. The Effective and Double doses of the drug reduced Total cholesterol along with levels of Triglycerides, Low density lipoprotein and Very low density lipoprotein levels significantly (p < 0.01) along with a significant (p < 0.01) increase in high density lipoproteins (HDL) in rats. There was also significant (p < 0.01) improvement in atherogenic indices like Castelli Risk Index I, Non HDL C/HDL, Castelli risk Index II, TG/HDL, Atherogenic coefficient and Atherogenic Index of Plasma. The combination of powdered sprouted mung bean and yam powder have excellent lipid lowering potential.

Search for medicinal plants to treat kidney disorders is an important topic on phytotherapeutical research. Plumbago zeylanica L. is an important medicinal plant with hepatoprotective, anti-inflammatory, anti-diabetic, anti-cancer and anti-hyperlipidemic activities. In the present study, the protective effect of hydroalcoholic extract of P. zeylanica (HAPZ) in cisplatin induced nephrotoxicity was analyzed in Swiss albino mice. Treatment with higher dose (400 mg/kg) of HAPZ significantly reversed the adverse effect of cisplatin on kidney weight, serum urea and creatinine, indicating their renoprotective effect. The antioxidant effect of the drug is evident from its significant effect on Catalase, Glutathione peroxidase and lipid peroxidation activities.

Helicanthes elastica (Desr.) Danser (Loranthaceae), commonly known as Indian mango mistletoe, is a parasitic shrub found widely growing on mango trees in southern India. Development of monographic quality standards is need of the hour for Pharmacopoeial/extra-Pharmacopoeial and folk medicinal plants. Systematic pharmacognostical evaluation of leaves of H. elastica has been carried out employing Pharmacopoeial procedures of testing herbal drugs. Macro-microscopic features of H. elastica leaf were recorded. Ethanolic extract was tested positive for alkaloids, steroids, carbohydrates, tannins, saponins and phenols. HPTLC fingerprint profile was developed for the identification of extracts using reference standard β -sitosterol glucoside. Results of the present investigation would serve as a source of pharmacognostical information and a document to control the quality of H. elastica (Desr.) Danser.

Mistletoes are hemiparasitic plants growing on aerial parts of other host trees. Many of the mistletoes are reported to be medicinally important. The hemiparasitic nature of these plants makes their chemical composition dependent on the host on which it grows. They are shown to exhibit morphological dissimilarities also when growing on different hosts. Helicanthus elastica (Desr.) Danser (mango mistletoe) is one such less explored medicinal mistletoe found on almost every mango tree in India. Traditionally, the leaves of this plant are used for checking abortion and for removing stones in the kidney and urinary bladder while significant antioxidant and antimicrobial properties are also attributed to this species of mistletoe. The current study was undertaken to evaluate molecular differences in the genomic DNA of the plant while growing on five different host trees using four random markers employing random amplified polymorphic DNA (RAPD) followed by similarity matrix by Jaccard's coefficient and distance matrix by hierarchal clustering analysis. Similarity and distance matrix data employing just 4 random markers, separately and the pooled data as well, revealed significant difference in the genomic DNA of H. elastica growing on five different hosts. Pooled data of similarity from all the 4 primers cumulatively showed similarity between 0.256 and 0.311. Distance matrix ranged from of 0.256 to 0.281 on pooling the data from all the four primers. The result employing a minimum number of primers could conclude that genomic DNA of H. elastica differs depending upon the host on which it grows, hence the host must be considered while studying or utilizing this mistletoe for medicinal purposes.