1. RNase Mitochondrial RNA Processing Cleaves RNA from the Rat Mitochondrial Displacement Loop at the Origin of Heavy-Strand DNA Replication
- Author
-
R. Michael Karwan, Walter Rossmanith, Elisabetta Sbisà, Apollonia Tullo, Esther-Maria Imre, and Cecilia Saccone
- Subjects
DNA Replication ,Mitochondrial RNA processing ,RNA-induced transcriptional silencing ,RNA, Mitochondrial ,RNase P ,Molecular Sequence Data ,RNA-dependent RNA polymerase ,In Vitro Techniques ,Biology ,Biochemistry ,Cell Line ,Substrate Specificity ,Mice ,Ribonucleases ,Animals ,Humans ,RNA Processing, Post-Transcriptional ,Binding Sites ,Base Sequence ,Intron ,RNA ,Non-coding RNA ,Molecular biology ,Mitochondria ,Rats ,Cell biology ,RNA silencing ,Nucleic Acid Conformation - Abstract
Ribonuclease mitochondrial RNA processing cleaves RNAs from the mammalian mitochondrial main non-coding regulatory region, called the displacement loop. Our data demonstrate that rat cells contain a site-specific ribonuclease mitochondrial RNA processing activity. We found that this enzyme processes the rat mitochondrial displacement-loop RNA substrate at the level of the conserved sequence block 1, a result which is different from that for mouse. This finding correlates with the in-vivo transcriptional analysis of the rat displacement-loop region. Processing by homologous and heterologous ribonuclease mitochondrial RNA enzymes occurs in the same manner, suggesting a conserved mode of substrate recognition.
- Published
- 1995
- Full Text
- View/download PDF