1. Calibration of optical tweezers with differential interference contrast signals
- Author
-
Giovanni Romano, Marco Capitanio, D. Dunlap, Francesco S. Pavone, L. Finzi, R. Ballerini, and M. Giuntini
- Subjects
Physics ,Time of flight ,Optics ,Optical tweezers ,Differential interference contrast microscopy ,business.industry ,Emphasis (telecommunications) ,Calibration ,business ,Interference (wave propagation) ,Anisotropy ,Instrumentation ,Noise (electronics) - Abstract
A comparison of different calibration methods for optical tweezers with the differential interference contrast (DIC) technique was performed to establish the uses and the advantages of each method. A detailed experimental and theoretical analysis of each method was performed with emphasis on the anisotropy involved in the DIC technique and the noise components in the detection. Finally, a time of flight method that permits the reconstruction of the optical potential well was demonstrated.
- Published
- 2002