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Bacterial spores from raw milk that survive the pasteurization process are responsible for half of all the spoilage of fluid milk. Bactofugation has received more attention as a nonthermal method that can reduce the presence of bacterial spores in milk and with it the spoilage of fluid milk. The objective of this work was to determine the effectiveness of bactofugation in removing spores from raw milk and estimate the effect the spore removal could have on shelf-life of fluid milk. The study was conducted in a commercial fluid milk processing facility where warm spore removal was performed using one-phase bactofuge followed by warm cream separation and high temperature, short time pasteurization. Samples from different stages of fluid milk processing with and without the use of bactofuge were tested for total plate count, mesophilic spore count, psychrotolerant spore count (PSC), and somatic cell count. Results were evaluated to determine the count reductions during different stages of fluid milk processing and compare counts in fluid milk processed with and without bactofugation. Bactofugation on average reduced the total plate count by 1.81 ± 0.72 log cfu/mL, mesophilic spore count by 1.08 ± 0.71 log cfu/mL, PSC by 0.86 ± 0.59 log cfu/mL, and somatic cell count by 135,881 ± 43,942 cells/mL. Psychrotolerant spore count in final pasteurized skim milk processed with and without bactofugation was used to predict the shelf-life of the pasteurized skim milk using the Monte Carlo simulation model. Although PSC in the initial raw milk was already low (-0.63 ± 0.47 log cfu/mL), the predicted values from the simulation model showed that bactofugation would extend the shelf-life of pasteurized skim milk by approximately 2 d. The results of this study will directly help fluid milk processors evaluate the benefits of using bactofugation as an intervention in their plants, and also demonstrate the benefits of using mathematical modeling in decision making.

DIII-D research is addressing critical challenges in preparation for ITER and the next generation of fusion devices through focusing on plasma physics fundamentals that underpin key fusion goals, understanding the interaction of disparate core and boundary plasma physics, and developing integrated scenarios for achieving high performance fusion regimes. Fundamental investigations into fusion energy science find that anomalous dissipation of runaway electrons (RE) that arise following a disruption is likely due to interactions with RE-driven kinetic instabilities, some of which have been directly observed, opening a new avenue for RE energy dissipation using naturally excited waves. Dimensionless parameter scaling of intrinsic rotation and gyrokinetic simulations give a predicted ITER rotation profile with significant turbulence stabilization. Coherence imaging spectroscopy confirms near sonic flow throughout the divertor towards the target, which may account for the convection-dominated parallel heat flux. Core-boundary integration studies show that the small angle slot divertor achieves detachment at lower density and extends plasma cooling across the divertor target plate, which is essential for controlling heat flux and erosion. The Super H-mode regime has been extended to high plasma current (2.0 MA) and density to achieve very high pedestal pressures (~30 kPa) and stored energy (3.2 MJ) with H 98y2 ≈ 1.6–2.4. In scenario work, the ITER baseline Q = 10 scenario with zero injected torque is found to have a fusion gain metric independent of current between q 95 = 2.8–3.7, and a lower limit of pedestal rotation for RMP ELM suppression has been found. In the wide pedestal QH-mode regime that exhibits improved performance and no ELMs, the start-up counter torque has been eliminated so that the entire discharge uses ≈0 injected torque and the operating space is more ITER-relevant. Finally, the high- (⩽3.8) hybrid scenario has been extended to the high-density levels necessary for radiating divertor operation, achieving ~40% divertor heat flux reduction using either argon or neon with P tot up to 15 MW.

A 1D radial diffusion model is developed to study the observed rapid expulsion of argon from the runaway electron plateau in the DIII-D tokamak following secondary massive low-Z ( D 2 or He) ga...

Areas of agreement and disagreement with present-day models of runaway electron (RE) evolution are revealed by measuring MeV-level bremsstrahlung radiation from runaway electrons (REs) with a pinhole camera. Spatially resolved measurements localize the RE beam, reveal energy-dependent RE transport, and can be used to perform full two-dimensional (energy and pitch-angle) inversions of the RE phase-space distribution. Energy-resolved measurements find qualitative agreement with modeling on the role of collisional and synchrotron damping in modifying the RE distribution shape. Measurements are consistent with predictions of phase-space attractors that accumulate REs, with non-monotonic features observed in the distribution. Temporally resolved measurements find qualitative agreement with modeling on the impact of collisional and synchrotron damping in varying the RE growth and decay rate. Anomalous RE loss is observed and found to be largest at low energy. Possible roles for kinetic instability or spatial tr...

Small (2–3 mm, 0.9–2 Pa · m3) argon pellets are used in the DIII-D tokamak to cause rapid shutdown (disruption) of discharges. The Ar pellet ablation is typically found to be much larger than expected from the thermal plasma electron temperature alone; the additional ablation is interpreted as being due to non-thermal runaway electrons (REs) formed during the pellet-induced temperature collapse. Simple estimates of the RE seed current using the enhanced ablation rate give values of order 1–10 kA, roughly consistent with estimates based on avalanche theory. Analytic estimates of the RE seed current based on the Dreicer formula tend to significantly underestimate it, while estimates based on the hot tail model significantly overestimate it.

We report on recent DIII-D experiments that integrate edge localized mode (ELM) suppression using resonant magnetic perturbations (RMPs) with divertor heat flux reduction under radiating divertor conditions. Our results illustrate the limitations in maintaining ELM suppression at gas puffing levels that were representative of good puff and pump operation in previous experiments without RMP. The electron pressure gradient in the pedestal (∇Pe) increased steadily during gas puffing and ELMs returned once ∇Pe reached values consistent with the peeling–ballooning stability limit, as determined by edge stability analysis. Even with this return of ELMs, a radiating divertor with RMP generated higher levels of total radiated power (∼40%) than comparable standard ELMing discharges without RMP at the same density. Differences in the accumulation of the seed argon in the core plasma between RMP and non-RMP during puff and pump were less than 20%.

Locked mode disruptions with a controlled toroidal phase are produced in the DIII-D tokamak by locking to large non-axisymmetric applied magnetic perturbations with different toroidal phases. The disruption conducted heat loads are found to reach almost completely the divertor region, possibly due to not only strong inner leg detachment but also plasma motion and limiting on the outer divertor leg shelf. The outer leg conducted heat loads are found to have a significant toroidal variation of order ±30%, with a dominant n = 1 structure. The heat load phase is shifted from the initial locked mode phase in a way that is approximately consistent with heat loss into the scrape-off layer being enhanced at the mode island O-point outer midplane crossing. These measurements suggest that pre-existing locked modes can affect the conducted heat load structure during the thermal quench by affecting the thermal quench MHD phase. This is consistent with previous MHD simulations which indicated that pre-disruption locke...

Fast visible imaging of the lower divertor from above is used to study the structure and dynamics of lobes induced by resonant magnetic perturbations (RMPs) in Edge-Localized Mode (ELM) suppression experiments in DIII-D. The best compromise between the amount of light and sharp imaging was obtained using emission at 601 nm from Fulcher band molecular deuterium. Multiple spatially resolved peaks in the D2 emission, taken as a proxy for the particle flux, are readily resolved during RMPs, in contrast to the heat flux measured by infrared cameras, which shows little spatial structure in ITER-like conditions. The 25 mm objective lens provides high spatial resolution (2–4 mm/pixel) from the centerpost to the outer shelf over 40° toroidally that overlaps the field of view of the IRTV that measures the divertor heat flux, allowing direct comparison in non-axisymmetric discharges. The image is coupled to a Phantom 7.3 camera using a Schott wound fiber bundle, providing high temporal resolution that allows the lobe dynamics to be resolved between ELMs and across ELM suppression onset. These measurements are used to study the heat and particle flux in 3D magnetic fields and to validate models for the plasma response to RMPs.

Recent progress in experimental and theoretical studies of edge localized mode (ELM) physics is reviewed for the reactor relevant plasma regimes, namely the high confinement regimes, that is, H-modes and advanced scenarios.Theoretical approaches to ELM physics, from a linear ideal magnetohydrodynamic (MHD) stability analysis to non-linear transport models with ELMs are discussed with respect to experimental observations, in particular the fast collapse of pedestal pressure profiles, magnetic measurements and scrape-off layer transport during ELMs.High confinement regimes with different types of ELMs are addressed in this paper in the context of development of operational scenarios for ITER. The key parameters that have been identified at present to reduce the energy losses in Type I ELMs are operation at high density, high edge magnetic shear and high triangularity. However, according to the present experimental scaling for the energy losses in Type I ELMs, the extrapolation of such regimes for ITER leads to unacceptably large heat loads on the divertor target plates exceeding the material limits. High confinement H-mode scenarios at high triangularity and high density with small ELMs (Type II), mixed regimes (Type II and Type I) and combined advanced regimes at high βp are discussed for present-day tokamaks. The optimum combination of high confinement and small MHD activity at the edge in Type II ELM scenarios is of interest to ITER. However, to date, these regimes have been achieved in a rather narrow operational window and far from ITER parameters in terms of collisionality, edge safety factor and βp.The compatibility of the alternative internal transport barrier (ITB) scenario with edge pedestal formation and ELMs is also addressed. Edge physics issues related to the possible combination of small benign ELMs (Type III, Type II ELMs, quiescent double barrier) and high performance ITBs are discussed for present-day experiments (JET, JT-60U, DIII-D) in terms of their relevance for ITER. Successful plasma edge control, at high triangularity (~0.5) and high density (~0.7nGR), in ITB scenarios in JET is reported.Active control of ELMs by edge current, pellet injection, impurities and external magnetic perturbations creating an ergodic zone localized at the separatrix are discussed for present-day experiments and from the perspective of future reactors.

The quiescent double barrier (QDB) regime is a high performance regime recently identified in DIII-D and characterized by a double transport barrier structure (core and edge) that can be maintained for several seconds, often limited only by the pulse length capabilities of the DIII-D hardware. The QDB regime has been sustained for up to 25 τE with fusion performance of up to βNH89≈7. The edge barrier is ELM-free, but modulated by low frequency MHD activity that allows density control via an external cryopump. The core barrier is similar to those seen in previous internal transport barrier experiments, but is maintained without complete stabilization of turbulence. Instead, the turbulence correlation lengths become very short so as to minimize the transport length scales. The two barriers are separated by a region of high transport that is a consequence of a zero-crossing in the E×B shearing rate. These discharges typically possess highly peaked density profiles. This has several implications: narrow bootstrap current profile, reduced beta limit and increased impurity retention. We will report on studies of each of these issues.

Experiments have been executed in the DIII-D tokamak to extend suppression of Edge Localized Modes (ELMs) with Resonant Magnetic Perturbations (RMPs) to ITER-relevant levels of beam torque. The results support the hypothesis for RMP ELM suppression based on transition from an ideal screened response to a tearing response at a resonant surface that prevents expansion of the pedestal to an unstable width [Snyder et al., Nucl. Fusion 51, 103016 (2011) and Wade et al., Nucl. Fusion 55, 023002 (2015)]. In ITER baseline plasmas with I/aB = 1.4 and pedestal ν * ∼ 0.15, ELMs are readily suppressed with co- I p neutral beam injection. However, reducing the beam torque from 5 Nm to ≤ 3.5 Nm results in loss of ELM suppression and a shift in the zero-crossing of the electron perpendicular rotation ω ⊥ e ∼ 0 deeper into the plasma. The change in radius of ω ⊥ e ∼ 0 is due primarily to changes to the electron diamagnetic rotation frequency ω e * . Linear plasma response modeling with the resistive MHD code m3d-c1 in...

Previous research has shown correlations between normal personality variables of the Sixteen Personality Factor Questionnaire (16PF) and Axis II personality disorder scales using the Morey, Waugh, and Blashfield Minnesota Multiphasic Personality Inventory (MMPI) and Millon Clinical Multiaxial Inventory. This study ( N = 37) compared variables from the adolescent version of the 16PF, the High School Personality Questionnaire, Revised, including the new Clinical Supplement and the MMPI scales of Morey, et al. and yielded results similar to those from earlier studies with other inventories. Extraversion scores correlated positively with those on Narcissistic and Histrionic scales, negatively with scores on Schizoid, Avoidant, and Schizotypal scales; scores on Independence had a similar pattern.

Spheroidin (SPH) is the most highly expressed gene of the entomopoxvirus isolated from Amsacta moorei (AmEPV). The level of expression of poxvirus genes is believed to be governed in large part by the promoter. Poxvirus promoters generally consist of approximately 40 bp which frequently terminate at the 3' end with a translation initiating TAAATG sequence. We have examined the requirements for high levels of SPH gene expression by constructing AmEPV recombinants containing either the SPH promoter or the late vertebrate poxvirus promoter derived from the cowpox virus A-type inclusion (ATI) gene. In addition, we have examined SPH promoter derivatives which extend beyond the 3' TAAATG to include 2 or 20 bp of the 5' coding sequence of the SPH gene. Examination of insect cells infected with these AmEPV ATI-lacZ or SPH-lacZ recombinants suggests that ATI-lacZ expression begins 12 h before and is essentially complete prior to any SPH-lacZ expression, allowing functional distinction between the ATI and SPH promoters and implying that different factors regulate the two promoters within the insect environment. SPH promoter-regulated expression is significantly enhanced within infected insect cells by including the additional 20 bp of the N-terminal SPH coding sequences as part of the promoter. However, when any of the SPH promoter constructs, including those containing the downstream sequences, were inserted into vaccinia virus, only very low levels of beta-galactosidase expression were observed. These results imply that downstream coding sequences within the SPH gene enhance SPH gene expression only within the insect environment.

What is the spread between expected and required returns on common equity for electric and combination electric and gas distribution utilities, using the market-to-book value ratio? We find that the M/B ratio is negatively associated with such factors as the level of long-term interest rates, and positively associated with the earned return on common equity and operation in a favorable regulatory climate.

This paper offers an empirical look at the corporate performance and capital structure of large enterprises from four emerging market economies of Asia. The paper combines two strands of business research: one from the international business field on corporate performance and country of origin; and the other from corporate finance research on capital structure. We study 81 corporations from Hong Kong, Malaysia, Singapore and Korea and find that both financial performance and capital structure are influenced by the country of origin. Specifically, we find that Hong Kong corporations have significantly higher returns on equity and invested capital than corporations from the other countries, possibly reflecting the concentrated conglomerate business structure typical of Hong Kong. The performance differences among firms from other countries are not statistically significant. Firms from Korea have significantly higher leverage than firms from the other countries. Leverage itself does not seem to affect corporate performance. The evidence lends only limited support to the extant capital structure theories in these emerging market economies.

This paper tests the mimicking propositions from signalling theory as they relate to stated firm objectives and firm performance. We classify the corporate objectives of a large sample of firms and evaluate firm performance relative to these objectives. We find that poorly performing firms more frequently cite shareholder wealth maximization as their primary objective than do better performing firms. There is no evidence that firms citing a shareholder wealth maximization objective perform any better than firms with alternative objectives. Similar evidence is found for other common corporate objectives. Overall, our results are consistent with signalling theory in that non‐enforceable signals, such as proclamations of corporate objectives, are not credible signals for investors.

This paper examines the determinants of capital structure of large corporations of industrialized countries (excluding financial institutions and regulated utilities), using five years of data ending in 1992. The study employs variables reflecting differing theoretical arguments on capital structure. We find evidence similar to previous empirical research using data for American companies. In particular, the pecking order theory of capital structure, with past profitability being the major determinant of leverage, is supported. For U.S. firms, but not for Japanese firms, tax factors also appear to be important determinants of capital structure. In general, variables proxying for firm size and growth also appear to be significant variables in explaining capital structure variations. Corporations from Germany and Italy appear to be most different from the American companies, with the former having lower and the latter higher leverage ratios relative to the U.S. corporations. Japanese companies appear to use less long‐term leverage, a result that is consistent with the close ties between Japanese firms and their banks — a relationship that permits a greater use of short‐term financing than is true in the U.S.

Rising shareholder activism following poor corporate performance and a subsequent drop in shareholder value at many major U.S. corporations had rekindled interest in duality and corporate governance. Despite limited empirical evidence, duality (chairman of the board and CEO are the same individual) has been blamed, in many cases, for the poor performance, and failure of firms to adapt to a changing environment. In examining the relationship between duality and firm performance, this study considers the announcement effects of changes in duality status, accounting measures of operating performance for firms that have changed their duality structure, and long-term measures of performance for firms that have had a consistent history of a duality structure. Our results suggest that : (1) the market is indifferent to changes in a firm's duality status ; (2) there is little evidence of operating performance changes around changes in duality status ; and (3) there is only weak evidence that duality status affects long-term performance, after controlling for other factors that might impact that performance.

Injection of large shattered pellets composed of variable quantities of the main ion species (deuterium) and high-Z impurities (neon) in the DIII-D tokamak demonstrates control of thermal quench (TQ) and current quench (CQ) properties in mitigated disruptions. As the pellet composition is varied, TQ radiation fractions increase continuously with the quantity of radiating impurity in the pellet, with a corresponding decrease in divertor heating. Post-TQ plasma resistivities increase as a result of the higher radiation fraction, allowing control of current decay timescales based on the pellet composition. Magnetic reconstructions during the CQ show that control of the current decay rate allows continuous variation of the minimum safety factor during the vertically unstable disruption, reducing the halo current fraction and resulting vessel displacement. Both TQ and CQ characteristics are observed to saturate at relatively low quantities of neon, indicating that effective mitigation of disruption loads by shattered pellet injection (SPI) can be achieved with modest impurity quantities, within injection quantities anticipated for ITER. This mixed species SPI technique provides a possible approach for tuning disruption properties to remain within the limited ranges allowed in the ITER design.

A theoretical model of the role of regulatory climate in the capital structure decisions of regulated electric utilities is developed that indicates managers can mitigate the consequences of unfavorable regulation by increasing the proportion of debt in the capital structure. The increase in leverage is limited by increased bankruptcy risk with higher levels of debt. The model predicts that utilities will react to their regulatory climate by adjusting capital structure. This behavior may be an undesirable consequence of the regulatory process. Empirical support for the model, both cross sectional and over time, is provided.

Repetitive bursting instabilities with strong frequency chirping occur in highbeta, beam-heated plasmas with safety factor q > 1 in the DIII-D tokamak. Although the mode structures differ, in many ways, the off-axis fishbones are similar to the q = 1 fishbones first observed on the Poloidal Divertor Experiment (PDX). The modes are driven by energetic trapped ions at the fastion precession frequency. During a burst, the frequency changes most rapidly as the mode reaches its maximum amplitude. Larger amplitude bursts have larger growth rates and frequency chirps. Unlike PDX fishbones, the decay phase is highly variable and is usually shorter than the growth phase. Also, the waveform is highly distorted by higher harmonics during the latter portion of a burst. The radial mode structure alters its shape during the burst. Like PDX fishbones, the modes expel trapped ions in a ‘beacon’ with a definite phase relationship relative to the mode. Seven types of loss detectors measure the beacon. The losses scale linearly with mode amplitude. The neutron rate changes most rapidly at maximum mode amplitude but, depending on the loss diagnostic, the losses often peak a few cycles later. The non-ambipolar fast-ion losses cause a sudden change in toroidal rotation frequency across the entire plasma. In addition to an overall drop, the neutron signal oscillates in response to the wave. Unlike the beacon of lost particles, which maintains a fixed phase relative to the mode, the phase of the neutron oscillations steadily increases throughout the burst, with the greatest phase slippage occurring in the highly nonlinear phase near maximum mode amplitude US Department of Energy SC-G903402, DE-FC02-04ER54698, DE-FG02-07ER54917

From 1976 to the present, electric utilities have earned returns that were either appreciably less or greater than their allowed equity return. The evidence suggests that equity earnings shortfalls can be explained in large part by inflation levels and changes in demand for electric power, as well as distortions in the regulatory process.

This article explores reasons for high observed dividend payout ratios and dividend yields in regulated electric utilities. We argue that monitoring of the capital markets, forced by high dividend payout rates, substitute for the agency control mechanisms that are largely absent for utility firms. We have tested the Smith hypothesis that utility firms use dividend policy as a mechanism for controlling or responding to regulatory risk. Our results are consistent with the Smith hypothesis and suggest that the dividend policies of regulated utilities are influenced significantly by policies adopted by various regulatory commissions.

In this paper the authors examine the common stock price behavior of firms that call their non-convertible preferred stock. The findings for the entire sample of preferred stock calls are consistent with the Modigliani and Miller (MM) leverage hypothesis that preferred stock financing adds no value to the firm. However, for those firms whose preferred stock was completely eliminated from the capital structure, a significant, positive announcement effect is observed. This finding is consistent with an information signaling effect related to the earnings prospects and tax status of the calling firms and also is suggestive of a burdensome covenant effect. No evidence is found to support the free cash flow theory of common stock price reactions to preferred stock calls.

The effect of resonant magnetic perturbations on heat transport in DIII-D H-mode plasmas has been calculated by combining the TRIP3D field line tracing code with the E3D two-fluid transport code. Simulations show that the divertor heat flux distribution becomes non-axisymmetric because heat flux is efficiently guided to the divertor along the three-dimensional invariant manifolds of the magnetic field. Calculations demonstrate that heat flux is spread over a wider area of the divertor target, thereby reducing the peak heat flux delivered during steady-state operation. Filtered optical cameras have observed non-axisymmetric particle fluxes at the strike point and Langmuir probes have observed non-axisymmetric floating potentials. On the other hand, the predicted magnitude of stochastic thermal transport is too large to match the pedestal plasma profiles measured by Thomson scattering and charge exchange recombination spectroscopy. The Braginskii thermal conductivity overestimates the experimental heat transport in the pedestal because the mean free paths of both species are longer than estimates of the parallel thermal correlation lengths, and collisionless transport models are probably required for accurate description. However, even the collisionless estimates for electron thermal transport are too large by one to two orders of magnitude. Thus, it is likely that another mechanism such as rotational screening of resonant perturbations limits the stochastic region and reduces transport inside of the pedestal.

Studies of submicron dust using Mie scattering from Nd:YAG lasers and video data of micron to sub‐millimeter sized dust on DIII‐D tokamak have provided the first data of dust sources and transport during tokamak discharges. During normal operation on DIII‐D dust observation rates are low, a few events per discharge or less. The net carbon content of the dust corresponds to a carbon atom density a few orders of magnitude below the core impurity density. Statistical analysis of Mie data collected over months of operation reveal correlation of increased dust rate with increased heating power and impulsive wall loading due to edge localized modes (ELMs) and disruptions. Generation of significant amounts of dust by disruptions is confirmed by the camera data. However, dust production by disruptions alone is insufficient to account for estimated in‐vessel dust inventory in DIII‐D. After an extended entry vent, thousands of dust particles are observed by cameras in the first 2–3 plasma discharges. Individual par...

This paper integrates research on the accuracy of alternative long-term earnings forecasts, the gain in accuracy achievable from combining various forecasts, and the power of different long-term earnings forecasts to explain stock prices. The tests are performed on 82 electric utility firms because of the relative homogeneity of accounting data in that industry and because of the importance of the findings for the determination of the cost of capital in a regulatory proceeding. The results are consistent with earlier research findings that analyst forecasts of long-term earnings growth are more accurate than forecasts from extrapolative models. Combined forecasts applied to out-of-sample data, however, did not result in markedly improved forecasting accuracy. Finally, valuation tests of alternative forecasting techniques offered strong evidence that investors place the greatest weight on forecasts from Value Line.

The spatial distribution of seagrass and algae communities can be difficult to determine in large, shallow lagoon systems where high turbidity prevents the use of optical methods like aerial photography or satellite imagery. Further complications can arise when algae are not permanently attached to the substratum and drift with tides and currents. A study using acoustic seafloor discrimination was conducted in the Indian River Lagoon (Florida, USA) to determine the extent of drift algae and seagrass. Acoustic surveys using the QTC View V system based on 50 and 200 kHz transducers were conducted near Sebastian Inlet. Results indicate that areas of seagrass can be identified, and are mixed with a high abundance of drift algae. Nearest-neighbor extrapolation was used to fill in spaces between survey lines and thus obtain spatially cohesive maps. These maps were then ground-truthed using data from towed video and compared using confusion matrices, The maps showed a high level of agreement (60%) with the actual distribution of algae, however some confusion existed between bare sand and algae as well as seagrass.

Intentionally triggered upward and downward vertical displacement events (VDEs) leading to disruptions were pre-emptively mitigated with neon massive gas injection (MGI) coming from either above or below the plasma. Global indicators of disruption mitigation effectiveness (conducted heat loads, radiated power, and vessel motion) do not show a clear improvement when mitigating with the gas jet located closer to the VDE impact area. A clear trend of improved mitigation is observed for earlier MGI timing relative to the VDE impact time. The plasma edge magnetic perturbation is seen to lock to a preferential phase during the VDE thermal quench, but this phase is not clearly matched by preliminary attempts to fit to the conducted heat load phase. Clear indications of plasma infra-red (IR) emission are observed both before and during the disruptions. This IR emission can affect calculation of disruption heat loads; here, the time decay of post-disruption IR signals is used to correct for this effect.

The entomopoxvirus from Amsacta moorei serves as the prototype of the group B entomopoxviruses. One of the interesting genes found in Amsacta moorei entomopoxvirus (AmEPV) is a superoxide dismutase ( sod ) (open reading frame AMV255). Superoxide dismutases (SODs) catalyze the conversion of superoxide radicals to hydrogen peroxide and oxygen. Many vertebrate poxviruses contain a sod gene, but to date, none have been demonstrated to be active. There are three families of SODs, characterized by their metal ion-binding partners, Fe, Mn, or Cu and Zn. Poxvirus enzymes belong to the Cu-Zn SOD family. Unlike inactive vertebrate poxvirus SODs, AMVSOD contains all the amino acids necessary for function. We expressed and purified a 6X-His-tagged version of the AMVSOD in Escherichia coli . The recombinant AMVSOD demonstrates superoxide dismutase activity both in an in situ gel assay and by stopped flow spectrophotometry. The k cat / K m for AMVSOD is 4 × 10 7 M −1 s −1 . In infected cells, the AMVSOD protein behaves as a dimer and is catalytically active; however, disruption of the gene in AMEPV has little or no effect on growth of the virus in cell culture. An analysis of mRNA expression indicates that AMV sod is expressed late during infection of Lymantria dispar (Ld652) cells and produces a discrete nonpolydisperse transcript. Characterization of protein expression with a monoclonal antibody generated against AMVSOD confirms that the AMVSOD protein can be classified as a late, postreplicative gene. Therefore, AMVSOD is the first example of an active poxvirus SOD.

The scientific goal of the UCSD-DIII-D Collaboration during this period was to understand the coupling of the core plasma to the plasma-facing components through the plasma boundary (edge and scrape-off layer). To achieve this goal, UCSD scientists studied the transport of particles, momentum, energy, and radiation from the plasma core to the plasma-facing components under normal (e.g., L-mode, H-mode, and ELMs), and off-normal (e.g., disruptions) operating conditions.

Previous observations that the highly conserved poxvirus-encoded serpins inhibit cytotoxic activities of alloreactive CTL via granule and/or Fas-mediated pathways was taken to indicate their involvement in immune evasion by poxviruses. We now show that interference with 51Cr release from target cells by ectromelia and cowpoxvirus is limited to alloreactive but not MHC-restricted CTL. The data are in support of the paramount importance of CTL and its effector molecule perforin in the recovery from primary ectromelia virus infection and question the role of serpins in the evasion of poxviruses from killing by CTL. Further analysis of poxvirus interference with target cell lysis by alloreactive CTL revealed that suppression primarily affects the Fas-mediated, and to a lesser extent, the granule exocytosis pathway. Serpin-2 is the main contributor to suppression for both killing pathways. In addition, inhibition of lysis was shown to be both target cell type- and MHC allotype-dependent. We hypothesize that differences in TCR affinities and/or state of activation between alloreactive and MHC-restricted CTL as well as the quality (origin) of target cells are responsible for the observed phenomenon.

Cytototoxic T lymphocyte-induced apoptosis can occur either through the directed exocytosis of granzyme B and perforin or via ligation of Fas. Both pathways involve the activation of a family of cysteine proteinases, the caspases, that cleave substrates at aspartic acid and are themselves activated by cleavage at internal aspartate residues. Fas recruits caspase 8, which initiates the death program through the subsequent activation of caspase 3. Granzyme B can process both caspase 8 and 3 in vitro, suggesting that both Fas and granzyme B access the apoptotic program in the same way. Here we demonstrate that although the two mechanisms are similar, the events that lead to activation of caspase 3 can be distinguished in vivo on the basis of their sensitivities to both pharmacological and virus-encoded caspase inhibitors. In cytotoxic T lymphocytes-mediated death the initial cleavage event on caspase 3 is insensitive to benzyloxycarbonyl-Val-Ala-Asp fluoromethyl ketone (zVAD-fmk) inhibition in both mouse and human systems. During Fas-mediated death, however, activation of caspase 3 is completely inhibited to zVAD-fmk. In addition, the viral serpin SPI-2, a homologue of cytokine response modifier A (crmA), is an effective inhibitor of the Fas but not the granzyme pathway. Our results demonstrate that whereas Fas-mediated activation of caspase 3 requires an upstream caspase activity that is zVAD-fmk-sensitive, the initial cleavage of caspase 3 during granule-mediated cell death is insensitive to zVAD-fmk, suggesting that caspase 3 is cleaved directly by granzyme B in vivo.

The effects of the serotonin agonist, quipazine, on the induction of c-fos in the suprachiasmatic nucleus of the rat was examined at different times of the 24 h cycle. Quipazine administered at night induced Fos production in a dose dependent manner (1, 3, 10, 30 mumol/kg) in the ventrolateral portion of the suprachiasmatic nucleus at ZT18. Administration of the highest dose at other times resulted in c-fos induction at ZT15 but not at other times of the day or subjective day examined (CT6 and ZT12). When compared to the effects of light pulses (2 lux/1 min), quipazine only caused c-fos induction at times when light caused induction. Our results support a role of serotonergic pathways in the transmission or modulation of photic information from the retina to the suprachiasmatic nucleus of the rat.

It is generally agreed that the primary scientific goal of the first near Sun fly-by mission is to identify the source of momentum responsible for driving the solar wind and to understand the physics of solar coronal heating. In this paper, we present an innovative concept for obtaining a unique set of data with which to better understand the physics of solar wind acceleration and coronal heating. The instrument, called the Solar Event Correlation Analyzer (SECA), operates on the principle that the topology of the open magnetic field lines can be used to causally correlate the evolution of small scale structures (events) in the Sun’s transition region with in situ solar probe measurements. These small-scale events are generally viewed as the mechanisms providing energy and momentum for the solar corona and wind, an assumption that will be conclusively tested with the SECA instrument. This paper describes the SECA concept and its scientific justification.