Search

Atopic dermatitis (AD) is a chronic skin disease that significantly impacts patient quality of life (QoL). It is unknown whether patients and physicians have the same interpretation of AD burden. Unmet needs and AD disease burden were evaluated by comparing terminology used in social media with terminology used in scientific literature. AD terminology in social media was identified using the NetBase platform, and natural language processing was performed. Topics and words driving negative sentiment were evaluated overall and in relation to specific symptoms. The systematic review of scientific literature identified publications that included AD and QoL terms was identified from PubMed. Term analysis of titles and abstracts was conducted via natural language processing. The occurrence of topics and co-occurrence of words associated with QoL terms were evaluated. More than 3 million social media mentions (2018-2020) and 1519 scientific publications (2000-2020) were evaluated. There were more negative than positive social media mentions, and flare and pain were common symptoms driving negative sentiment. Face and hands were major drivers of negative sentiment in relation to AD symptoms in social media. Sleep and pain were often mentioned together. In scientific literature, pruritus and depression were the most frequently occurring symptoms. Similarly, pruritus was the most common AD symptom co-occurring with QoL terms in the assessed scientific literature. Social media analyses provide a unique view into the patient experience of AD. Symptoms driving negative sentiment in social media appear to be discordantly represented in scientific literature. Incorporating patient perspectives may improve disease understanding and management.

El objetivo del presente trabajo fue evaluar el efecto de la inclusión de hojas de Acacia cochliacantha en una dieta de mantenimiento para cabritos de la raza Boer en el conteo fecal de huevos (CFH) de Haemonchus contortus, consumo de agua y materia seca. Diez cabritos recién destetados (16.850 ± 1.630 kg de peso vivo inicial y 3 meses de edad), fueron experimentalmente infestados con larvas (L3) de H. contortus (350 larvas por kilogramo de peso vivo), para probar dos tratamientos: T1: testigo (animales infectados con larvas L3 de H. contortus, sin inclusión de hojas de A. cochliacantha) y T2: animales infectados con larvas L3 de H. contortus, con inclusión de 5% hojas de A. cochliacantha en la dieta. Antes de asignar los tratamientos a los animales, se realizó un CFH por gramos de heces en cada uno de ellos y de esta manera agruparlos de mayor a menor en relación a la cantidad de huevos presentes en las heces, en los dos animales con valores más altos se asignaron aleatoriamente T1 o T2 y así sucesivamente hasta completar cinco repeticiones verdaderas para cada tratamiento. Las variables medidas fueron: CFH (por gramo de heces), consumo de agua y consumo de materia seca (CMS). Los resultados encontrados demostraron reducción (P 0.05). Se concluye que la adición de hojas de A. cochliacantha en dietas para cabritos tienen actividad antihelmíntica, por lo que esta leguminosa arbórea podría representar una opción en el manejo integral de la nematodiasis de cabritos Boer en crecimiento.

To describe the creation of an original 3D-printed liquid latex model designed for laparoscopic pyeloplasty (LP) simulation in infants, and to assess its usefulness.A 3D model of a dilated pelvis and a ureter with ureteropelvic junction obstruction (UPJO) was designed. It was covered in liquid latex, which allowed flexible models to be achieved in order to conduct pyeloplasty in a pelvitrainer. The total price of each model was 6 euros. A nearly-experimental, non-randomized, blind study was carried out, while measuring operating times and OSATS (Objective Structured Assessment of Technical Skills) scores. Following simulation completion, a survey based on Likert scale was conducted to assess overall appearance, texture, usefulness, and probability of recommending the model for regular training.8 pediatric surgeons spent a median of 71.5 minutes (range: 50-86), and rated the model with a median 20.1/30 (range: 17-24) OSATS score. The model received a 4.25 (range: 3-5) score in terms of overall appearance, a 4.37 (range: 3-5) score in terms of texture, a 4.5 (range: 4-5) score in terms of usefulness, and a 4.6 (range: 4-5) score in terms of probability of recommending the model for regular training.Our liquid latex model for laparoscopic pyeloplasty simulation is feasible, with favorable preliminary results. Its usefulness in laparoscopic pyeloplasty training is promising.Describir la creación de un modelo original de látex líquido diseñado para la simulación de la pieloplastia laparoscópica (PL) en lactantes, construido a partir de una impresión tridimensional (3D), y valorar su utilidad.Se diseñó un modelo 3D de una pelvis dilatada y un uréter con estenosis pieloureteral (EPU), que fue recubierto por látex líquido obteniendo modelos flexibles para realizar la pieloplastia en un pelvitrainer. El precio total de cada modelo fue de 6 euros. Se realizó un estudio cuasiexperimental, ciego y no aleatorizado, midiendo el tiempo quirúrgico y la puntuación OSATS (Objective Structured Assessment of Technical Skills). Tras completar la simulación, se realizó una encuesta utilizando la escala de Likert, en la cual se valoró el aspecto general, la textura, la utilidad del modelo y el grado de recomendación en el entrenamiento habitual.8 cirujanos pediátricos dedicaron una mediana de 71,5 minutos (R 50-86), y puntuaron una mediana de 20,1/30 (R 17-24) en la escala OSATS. El modelo obtuvo una valoración de 4,25 (R3-5) en aspecto general, 4,37 (R3-5) en textura, 4,5 (R 4-5) en utilidad y 4,6 (R 4-5) en recomendación para incorporar al entrenamiento habitual.El uso de nuestro modelo de látex líquido para la simulación de la pielopalstia laparoscópica es factible y los resultados preliminares han sido favorables. Su utilidad como herramienta en el entrenamiento de la pieloplastia laparoscópica es prometedora.

Opicapone is a catechol-O-methyl-transferase (iCOMT) inhibitor authorized in Europe in 2016 and indicated as adjunctive therapy to preparations of levodopa/ DOPA decarboxylase inhibitors in adult patients with Parkinson's disease and end-of-dose motor fluctuations who cannot be stabilised on those combinations. The efficacy of opicapone in these patients has been demonstrated in two pivotal randomized clinical trials, BIPARK I and BIPARK II, in which it has demonstrated its superiority versus placebo and non-inferiority versus entacapone. Although they constitute the gold standard for the evaluation of interventions, randomized clinical trials present limitations of external validity due to the use of strict eligibility criteria. Therefore, it is considered necessary to have a more comprehensive evaluation of the efficacy of the drug, complementing the information obtained from randomized clinical trials with that of "real world or real clinical practice" studies. The objective of this review has been to collect and put into perspective the information available on opicapone coming from real clinical practice studies in Spain. The data from Spain with opicapone in 18 series with more than 1,000 patients in total, confirm the safety and efficacy previously reported with this iCOMT. Furthermore, they show that opicapone is especially useful in patients with a less advanced stage of the disease and mild motor fluctuations, which would suggest that the earlier its introduction in the therapeutic scheme for the management of motor fluctuations, the better is the benefit-risk ratio for the drug.Opicapona para el tratamiento de la enfermedad de Parkinson: datos de vida real en España.Resumen. La opicapona es un inhibidor de la catecol-O-metiltransferasa (iCOMT) autorizado en Europa en 2016 como terapia adyuvante a las preparaciones de levodopa/inhibidores de la dopa descarboxilasa en pacientes adultos con enfermedad de Parkinson y fluctuaciones motoras de final de dosis que no puedan ser estabilizados con esas combinaciones. La eficacia de la opicapona en estos pacientes ha sido demostrada en dos ensayos clínicos pivotales, BIPARK I y BIPARK II, en los que se ha demostrado la superioridad frente al placebo y la no inferioridad frente a la entacapona. A pesar de que constituyen el estándar para la evaluación de intervenciones, los ensayos clínicos aleatorizados presentan limitaciones de validez externa debidas a la utilización de criterios estrictos de elegibilidad. Por tanto, se considera necesario disponer de una evaluación más amplia de la eficacia general del fármaco, complementando la información de los ensayos clínicos aleatorizados con estudios de ‘vida real o práctica clínica real’. El objetivo de esta revisión ha sido recopilar y poner en perspectiva la información disponible sobre los resultados de la opicapona en estudios de práctica clínica real en España. Los datos acumulados en España con opicapona en 18 series con más de 1.000 pacientes confirman la seguridad y la eficacia de este iCOMT comunicadas previamente. Además, muestran que la opicapona es especialmente útil en pacientes en un estadio de la enfermedad menos avanzado y fluctuaciones motores leves, lo que sugeriría una mejor relación beneficio-riesgo cuanto más temprana sea su introducción en el esquema terapéutico para el tratamiento de las fluctuaciones motoras.

Leaf mottle and wilt (LM) of sunflower caused by Verticillium dahliae is the most important disease in Argentina. LM is a monocyclic disease where microsclerotia in the soil are the primary inoculu...

Twenty-four Dorper x Pelibuey lambs were housed in individual pens during a 31-d feeding period and assigned to four treatments (n = 6) under a randomized complete block design with different daily doses of zilpaterol hydrochloride (ZH): 0 (control), 0.1, 0.2, and 0.3 mg/kg BW to determine the effects of ZH supplementation on productive performance, meat quality, and wholesale cut yields. Final BW (p = 0.02) and average daily gain (ADG, p = 0.04) were greater in lambs supplemented with 0.2 mg/kg BW. Supplemental ZH tended to improve dry matter intake (DMI, p = 0.008) and ADG:DMI ratio (p = 0.078). Wholesale cut yields were not affected by ZH supplementation. Percentage of head was greater (p = 0.04) in lambs treated with ZH. The ZH supplementation did not affect carcass characteristics. However, longissimus thoracis et lumborum (LTL) presented a linear trend (p = 0.08) of increasing with ZH supplementation. Percentage of blood presented a trend (p = 0.051) of decreasing with ZH supplementation. Also, liver decreased in size (p &lt, 0.05) for treatments where ZH was included. Values of luminosity decreased (p &lt, 0.02) when ZH dosage increased. The value of protein Lowry was greater, with 0.3 mg kg&minus, 1 (p = 0.04). Cathepsin B + L was greater in the lambs from the control treatment (p = 0.05). In conclusion, using a daily ZH dosage of 0.2 mg per kg of BW produced the best productive performance, carcass characteristics, and some changes in the meat of hair-breed lambs.

Surgical site infection (SSIs) in lower extremity vascular procedures is a major contributor to patient morbidity and mortality. Despite previous advancements in preoperative and postoperative care, the surgical infection rate in vascular surgery remains high, particularly when groin incisions are involved. However, successfully targeting modifiable risk factors reduces the surgical site infection incidence in vascular surgery patients. We conducted an extensive literature review to evaluate the efficacy of various preventive strategies for groin surgical site infections. We discuss the role of preoperative showers, preoperative and postoperative antibiotics, collagen gentamicin implants, iodine impregnated drapes, types of skin incisions, negative pressure wound therapy, and prophylactic muscle flap transposition in preventing surgical site infection in the groin after vascular surgical procedures.

Agrosilvopastoral systems that integrate crops, pastures, trees and shrubs are seen as a way forward to meet future needs for food, feed, fuel, and other products, as well as for providing environmental and social benefits. Cattle production systems in the tropical and subtropical areas of Mexico have a dual-purpose (milk and beef) objective in agrosilvopastoral systems that need to improve the production of goods, as well as non-productive outputs like environmental services, enhancing their sustainability. The aim of this study was to characterise the socioeconomic, and productive characteristics of dual purpose farms based on agrosilvopastoral systems (ASPS), in a subtropical region in the southern highlands of central Mexico, to contribute in the understanding of how these ASPS operate and their differences. Forty-seven farmers answered a semi-structured questionnaire to identify aspects of farm management, structure, and land use; as well as technical and economic aspects. Information was analysed using factorial analysis of principal components, in order to reduce information, and subsequently cluster analysis that included nine variables resulting into four groups of farms differentiated by structure, size, management and productive orientation. Productive orientation of the farms was from the activity from which farms obtained most of their incomes. The four groups were beef production oriented (BPO) 46.81%, milk production oriented (MPO) 23.40%, pure breed weaned calves (PBWC) 17.02%, and traditional dual-purpose (TDP) 12.77%. Characterization of agrosilvopastoral farms identified main differences and characteristics, as well as the presence and use of trees and shrubs by farmers and cattle.

Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Rojo-Rubio R, Gonzalez-Cortazar M, Olmedo-Juarez A, Zamilpa A, Arece-Garcia J, Mendoza-Martinez GD, Lee-Rangel HA, Vazquez-Armijo JF, Mendoza-de Gives P. Caesalpinia coriaria fruits and leaves extracts possess in vitro ovicidal activity against Haemonchus contortus and Haemonchus placei . Veterinaria Mexico OA. 2019;6(4). doi: 10.22201/fmvz.24486760e.2019.4.601. The ovicidal effect of hydro-alcoholic extracts from mature fruits and dried leaves of the Caesalpinia coriariatree , against Haemonchus contortus and H. placei was evaluated by the egg hatching inhibition test (EHIT). Secondary compounds within the extracts were identified by high performance liquid chromatography. Five extract concentrations were assessed in the inhibition tests (25.0, 12.5, 6.2, 3.1 and 1.5 mg/mL), using distilled water and Thiabendazole (0.5 mg/mL) as negative and positive controls, respectively. The results were analysed under a 2 × 2 × 6 completely randomized factorial design, and mean differences were established by the Tukey’s post hoc test. The 50% (EC 50 ) and 90% (EC 90 ) effective concentrations for each extract were determined using a Probit analysis.A concentration-dependent ovicidal effect of hydro-alcoholic extracts from both leaves and fruits was observed, reaching a 100% efficacy against both nematode species with the 25.0 mg/mL concentration (p < 0.05). The EC 50 for the fruit and leaves extracts were established as 1.63 and 3.91 mg/mL, and as 3.98 and 11.68 mg/mL, against H. contortus and H. placei , respectively. The most important secondary compounds identified in extracts were free-condensed tannins, gallic acid and methyl-gallate.In conclusion,hydro-alcoholic extracts from C. coriaria mature fruits and dried leaves inhibit in vitro egg hatching of H. contortus and H. placei .

Two experiments were completed to determine the dietary optimal dose of a generic product (Grofactor®) of zilpaterol hydrochloride (ZH) in fattening male lambs. In experiment 1, 32 lambs were assigned to four daily doses of ZH (n = 8): 0 (Control), 0.10, 0.20, and 0.30 mg/kg of body weight (BW). In experiment 2, the doses of ZH with the best biological response from experiment 1 (i.e., 0.10 and 0.20 mg/kg of BW per lamb) were compared using 18 lambs (9 lambs per treatment), as well as a control group of 9 lambs. In experiment 1, growth rate, feed efficiency, hot (HCW) and cold (CCW) carcass weight and dressing percentage showed a quadratic increase (P ≤ 0.05) as the dose of ZH increased, but all these traits were similar among ZH-fed lambs. Yield of wholesale cuts was not impacted by ZH dose. Percentages of skin and foot decreased linearly (P ≤ 0.01) with increasing ZH dose. However, head, liver, and stomach had a quadratic effect (P ≤ 0.05) to increasing levels of ZH, with the lowest values at the intermediate ZH addition levels. In experiment 2, ZH-fed lambs compared with control had better (P ≤ 0.04) feedlot performance, HCW, CCW, dressing percentage, Longissimus dorsi muscle area and carcass length. Addition of ZH only increased (P ≤ 0.03) the leg yield using any of the doses. Feeding ZH decreased (P ≤ 0.03) the percentages of blood, liver, spleen, and stomach, while percentage of lungs increased (P

Objective This experiment was designed to evaluate the effect of supplementation with soybean or canola oil on milk production and the composition of long chain fatty acids as well as weight changes in the goats and their kids. Methods Thirty nine mulitparous crossed Alpine×Nubian goats (initial body weight [BW] 43.5±1.7 kg) from the day of parturition were assigned to the treatments: grazing control (n = 15); grazing plus 20 mL/goat/d of supplemental soybean oil (n = 12); and grazing plus 20 mL/goat/d of supplemental canola oil (n = 12) from November 26, 2014 to March 7, 2015. The planned contrasts were: CI (control vs supplemented with oils); CII (soybean vs canola oil) to compare the treatment effects. Results The vegetable oil supplementation reduced weight losses in lactating goats (CI: −0.060 vs 0.090 kg/d; p = 0.03) but did not improve milk production or affect kids’ growth. The content of C4, C6, C8, C10, C11, C14, and C18:1n9t in the milk was increased (p

Fourteen Dorper × Pelibuey ram lambs (initial body weight [BW] = 37.4 ± 1.0 kg and age = 4.5 mo) were housed in individual pens during a 30-d feeding period, and then slaughtered to determine the effects of zilpaterol hydrochloride (ZH) supplementation on productive performance, carcass characteristics and wholesale cut yields. Lambs were assigned under a randomized complete block design (initial BW as blocking factor) to one of two dietary treatments: basal diet without (control) or with 10 mg daily of ZH/lamb. Lambs fed ZH had greater (P ≤ .04) final BW, average daily gain and dry matter intake, but similar (P = .24) feed efficiency compared with control lambs. Hot and cold carcass weight, dressing percentage, longissimus muscle area and leg perimeter were greater (P ≤ .05) for ZH-fed lambs than for control lambs. With exception of blood percentage which decreased (P

Summary This study determined the efficacies of four classes of anthelmintics (AH) in a goat flock where anthelmintic resistance (AR) to ivermectin was suspected. We selected and randomly distributed 105 animals with >500 eggs per gram of feces into seven groups of 15 animals: an untreated control group and groups treated with ivermectin, eprinomectin, albendazole sulfoxide, albendazole, levamisole, and closantel. The goats were individually weighed and treated with the recommended dose. Fecal samples were collected 14 days post-treatment to determine the fecal egg counts and for a fecal egg count reduction test (FECRT). Coprocultures were also performed for identifying any resistant genera. A molecular assay (polymerase chain reaction, PCR) was used to confirm benzimidazole resistance. The FECRTs for the ivermectin, eprinomectin, albendazole sulfoxide, and albendazole treatments were Haemonchus spp. Levamisole had a FECRT confidence interval

Ethnopharmacology relevance Acacia cochliacantha is a small tree whose foliage is traditionally used in Mexico for treatment of kidney pain, gastrointestinal illnesses and to kill intestinal parasites. In recent decades, the study of vegetal extracts has offered other possible alternatives for the control of Haemonchus contortus . Considering that this nematode affects dramatically the health and productivity of small ruminants, the aim of this study was to identify the anthelmintic compounds from A. cochliacantha hydro-alcoholic extract (HA-E) through an ovicidal test. Material and methods In vitro egg hatch assay was conducted to determinate the anthelmintic effects of a HA-E (60 g). Liquid-liquid ethyl acetate/water extraction gave two fractions (EtOAc-F, 1.92 g; Aq-F; 58.1 g). The less polar compounds from ethyl acetate fraction were extracted by addition of dichloromethane offering a precipitate phase (Mt-F, 1.25 g) and a soluble mixture (DCMt-F 1.15 g). All fractions were evaluated for ovicidal activity obtaining the egg hatching inhibition (EHI, 0.07–25 mg/mL). Ivermectin (0.5 mg/mL) was used as a reference drug (positive control), and distilled water, 2.5% DMSO and 2% methanol were used as negative controls. The isolated compounds from the most active fractions were subjected to spectroscopic ( 1 H NMR) Spectrometric (MS) and UV HPLC analysis in order to identify the bioactive compounds. Results The less polar treatments (AcOEt-F, DCMt-F, DCMt-P) showed the highest ovicidal activities (98–100% EHI; at 0.62–1.56 mg/mL) and the major compounds found in these fractions were identified as caffeoyl and coumaroyl derivatives, including caffeic acid ( 1 ), p -coumaric acid ( 2 ), ferulic acid ( 3), methyl caffeate ( 4 ), methyl- p -coumarate ( 5 ), methyl ferulate ( 6 ) and quercetin. In case of the less active fractions (Aq-F, Mt-F) were constituted principally by glycosylated flavonoids. Conclusion These results show that caffeoyl and coumaroyl derivatives from Acacia cochliacantha leaves had promising anthelmintic activity against Haemonchus contortus . This leguminous may offer an alternative source for the control of gastrointestinal nematodes of small ruminants.

Resumen Objetivos Presentamos nuestra casuistica de urolitiasis infantiles, tecnicas empleadas en su tratamiento y sus resultados. Material y metodos Estudio retrospectivo de urolitiasis infantiles de tracto urinario superior (TUS) tratadas en nuestro centro entre 2003-2014. Recogimos datos demograficos, clinicos, diagnosticos, terapeuticos y complicaciones. El plan terapeutico fue recogido como procedimiento aislado (litotricia extracorporea, ureterorrenoscopia, nefrolitotomia o cirugia) o terapia combinada. Resultados Estudiamos 41 unidades renoureterales en 32 pacientes. La mediana de edad se situo en 5 anos (rango 11 meses-14 anos). El tamano medio fue de 12,9 mm (± 7,3 mm). La localizacion: 23 (56%) en pelvis renal (coraliformes en 15 casos), 10 (24%) en caliz inferior y 8 (20%) ureterales. Realizamos 80 procedimientos, sin diferencias en los grupos de edad, encontrando 12 complicaciones (15%), sin presencia de ningun cuadro septico secundario a litotricia. Las litiasis en localizacion ureteral tuvieron un 100% de tasa de exito con la ureterorrenoscopia. El porcentaje de curacion global fue del 90%. Conclusion El abordaje de la urolitiasis en la infancia ofrece multiples alternativas, por lo que es importante individualizar en funcion del tamano, localizacion y composicion del calculo. En nuestro centro la utilizacion de LEOC en ninos es segura. La URS, semirrigida o flexible, obtiene excelentes resultados en el ureter. La NLP con minimo acceso se puede realizar en ninos pequenos y lactantes.

Objectives We present our case studies on pediatric urolithiasis, the techniques employed in its treatment and its results. Material and methods A retrospective study of pediatric urolithiasis of the upper urinary tract (UUT) treated at our center between 2003 and 2014. We recorded demographic, clinical, diagnostic and therapeutic data and the complications. The therapeutic plan was recorded as isolated (extracorporeal lithotripsy, ureterorenoscopy, nephrolithotomy or surgery) or combined therapy. Results We examined 41 renoureteral units in 32 patients. The median age was 5 years (range, 11 months–14 years). The mean size was 12.9 cm (±7.3 mm). The locations were as follows: 23 (56%) in the renal pelvis (staghorn in 15 cases), 10 (24) in lower calyx and 8 (20%) in the ureter. We performed 80 procedures, with no differences in the age groups, which resulted in 12 complications (15%) but no septic condition secondary to lithotripsy. Stone removal from the ureter had a 100% success rate with the ureterorenoscopy. The overall cure rate was 90%. Conclusion The pediatric urolithiasis approach offers multiple alternatives. It is therefore important to tailor the procedure according to the size, location and composition of the stone. In our center, the use of pediatric extracorporeal shock wave lithotripsy is safe. Ureterorenoscopy, semirigid or flexible, provides excellent results in ureters. Percutaneous nephrolithotomy with minimal access can be performed on small children and nursing infants.

The objective of this study was to evaluate the effects of natural zeolite on the performance and nitrogen metabolism of Rambouillet ewe lambs. Forty Rambouillet ewe lambs with an initial weight of 41.06 ± 3.9 kg were randomly assigned to one of four experimental diets, namely 0, 20, 40 and 60 g of natural zeolite (Comercializadora Omega® Mexico) per kg dietary dry matter in a completely randomized design and sixteen ewe lambs were used in a 4 x 4 Latin square metabolism study. Performance was not modified by natural zeolite levels in the diet, and daily gain showed a quadratic response. Ammonia concentration at 8 and 12 hours increased linearly with zeolite. Zeolite increased ruminal pH linearly at 8 and 12 hours. Acetate and butyrate presented a quadratic response, and total ruminal volatile fatty acid concentration was linearly increased by zeolite. Nitrogen retention showed a linear and quadratic response to zeolite. The estimated fractions of protein digested in the small intestine, when rumen-fermentable energy was limiting (PDIE) and when rumen-fermentable nitrogen was limiting, showed a linear and quadratic response to zeolite. PDIE was positively correlated (r = 0.96) with average daily gain. Keywords : Sheep, ammonium, ruminal fermentation, productive, soluble nitrogen

Acacia farnesiana pods are rich in secondary metabolites and their biological activities have been recorded as antibacterial, antioxidant and anthelmintic. Previously, an in vitro bioguided study showed the important ovicidal and larvicidal effects of an organic fraction (EtOAc-F) from a hydroalcoholic extract of A. farnesiana pods against Haemonchus contortus. The present study aimed to assess the in vivo anthelmintic effect of EtOAc-F from A. farnesiana pods on the H. contortus faecal egg elimination in female lambs and on the infective larvae (L3) population reduction in coprocultures. The EtOAc-F was obtained from a hydroalcoholic extract from A. farnesiana pods through chromatographic procedures; additionally, some secondary compounds were identified using high-performance liquid chromatography (HPLC). Twenty-one ‘Katahdin’ crossbred female lambs ranging from three to four months of age, with body weights 21.9 ± 0.39 kg were used. Animals were orally infected with H. contortus (L3) by a single dose of 350 L3/kg BW. Three experimental groups (n = 7) were assigned as follows: 1) Control (untreated), 2) Albendazole, as a positive control (at 7.5 mg/kg BW, unique dose) and 3) EtOAc-F (at 100 mg/kg BW, once every third day, with three applications in total). Individual faecal samples were collected once a week for 5 weeks (at days 38, 45, 52, 59 and 66) post-treatment, to measure the faecal egg counts (FEC) and to obtain the H. contortus (L3) population from faecal cultures. The highest FEC reduction caused by EtOAc-F was 67.7%; meanwhile, albendazole showed a total FEC reduction after the second week post-treatment (day 45). On the other hand, the fraction caused an important reduction in the larval population in coprocultures (54.3–68.5%). The phytochemical analysis revealed the presence of galloyl derivatives and flavonoids as major compounds. The A. farnesiana pods could serve as a natural anthelmintic for the control of H. contortus, and perhaps for controlling other parasites of veterinary importance.

The objective of this study was to evaluate the effects of three fibrolytic enzyme products (cellulase (CEL), xylanase (XYL) and a 1:1 mixture of CEL and XYL (MIX)) at three dose levels (0, 1 and 3 μL/0.5 g DM) on the in vitro fermentation of a diet for growing lambs. Bottles were incubated for 96 h at 39 °C. A mathematical model was used to estimate the parameters describing the gas production (GP) curve (b, c and L). Dry matter degradability (DMD) and fibre (NDFD and ADFD) degradability were determined at the end of the incubation period. Metabolisable energy (ME) and short chain fatty acids (SCFA) were calculated at 24 h of incubation. The asymptotic GP (parameter b) was affected (p

Resumen Objetivo Identificar el efecto que tiene la terapia combinada de compresion isquemica con estiramiento poscontraccion isometrica aplicada en puntos gatillo ubicados en el musculo trapecio superior, en la recuperacion del rango de movimiento cervical. Material y metodo Estudio de tipo cuantitativo, cuasi experimental. La muestra la componen 26 trabajadoras de oficina previamente diagnosticadas con puntos gatillo en el musculo trapecio superior y que no presentaran alguna patologia cervical previa. Primero se les midio el rango de movimiento cervical, luego se aplico tecnica placebo o experimental al azar y, posteriormente, se volvio a medir el rango de movimiento. Luego de 7 dias se aplico la tecnica no realizada en la primera intervencion, midiendo nuevamente el rango de movimiento antes y despues de aplicada la tecnica. En el analisis estadistico se compararon los resultados del antes y despues intertecnica e intratecnica, considerando una p Resultados La tecnica experimental mostro tener un aumento significativo, al igual que la tecnica placebo, ambos para una p de 0,05, pero al comparar la tecnica placebo con la tecnica experimental esta ultima mostro ser significativamente mayor a la placebo para una p de 0,05. Conclusiones La tecnica experimental aplicada sobre puntos gatillo ubicados en el musculo trapecio superior tiene mayor efectividad en la recuperacion del rango de movimiento cervical de inclinacion lateral frente a la tecnica realizada en el grupo placebo.

Accounting and mathematical modeling methods were combined to analyze the cost structure and economic optimization of small-scale dairy production systems in the present study. Information on the technical, economic, and production characteristics of 72 farms was obtained over a two-year period. Four strata of farms were defined using a random stratified sampling design with proportional allocation. To evaluate production costs, two accounting methods were used: (1) the activity-based budgeting (AB) method, which counted the actual cost of feed cultivation and family labor (FL) as a percentage of total feed and labor costs for each stratum, and (2) the standard accounting (SA) method, which estimated production costs according to the cost of purchasing 100% of feed and hiring 100% of labor. Based on the results of the AB method, four scenarios were developed and adapted to a mixed integer linear programming model to predict the percentage of feed cultivation and FL that maximizes economic benefits. The base scenario evaluated the actual percentages of feed cultivation and FL employed by each farm category, and scenarios I, II, and III simulated the purchase of feed and hire of labor at 20%, 50%, and 100%, respectively. The results showed that 100% dependence on the purchase of feed and hire of labor increases the daily production cost by 20% compared to the base scenario in the four evaluated strata. The mathematical model indicated that the production of less than 90% of feed and use of less than 80% family labor significantly decrease the income in each analyzed stratum. Accordingly, production under the simulated conditions of scenarios I, II, and III is economically and financially non-viable.

Ethnopharmacology relevance Acacia farnesiana (L.) Willd is a shrub legume used as condiment, medicinal plant and bioactive herbage. This species is used in traditional medicine of several countries to relieve the symptoms of gastrointestinal diseases, diarrhoea, stomach pain and typhoid as well as astringent, antidysenteric and anthelmintic. Some studies have shown that this plant displayed anthelmintic activity against several gastrointestinal nematode parasites of livestock, and also against parasites of human beings, such as malaria. Aim of the study This work describes the isolation and chemical identification of the anthelmintic compounds of Acacia farnesiana pods against eggs and infective larvae of the sheep parasitic nematode Haemonchus contortus. The bio-guided chemical fractioning of A. farnesiana pods using ethyl acetate against H. contortus eggs and infective larvae allowed for the identification of naringenin 7-O-(6″-galloylglucoside) (flavonol group) as the compound responsible for the anthelmintic activity against this important parasitic nematode. Materials and methods Anthelmintic activity was assessed using the egg hatching inhibition assay (EHI) and mortality tests. A complete hydroalcoholic extract (HA-E) at 12.5–50 mg/mL, an aqueous fraction (Aq-F) at 3.12–25 mg/mL and an ethyl acetate fraction (EtOAc-F) at 3.12–25 mg/mL were analysed in the first selection phase. The purification of compounds through the chromatographic separation of the organic fraction resulted in nine less complex mixtures (C1F1, C1F2, C1F3, C1F4, C2F1, C2F2, C2F3, C2F4 and C2F5) that were assessed at 0.62–5 mg/mL concentrations. In addition, thiabendazole (0.6 mg/mL) and ivermectin (5 mg/mL) were used as positive controls. Likewise, distilled water and 4% methanol were used as negative controls. The bioactive compounds of EtOAc-F were obtained and characterised through chromatographic processes like open column chromatography, thin layer chromatography (TLC), high performance liquid chromatography (HPLC), ultra-performance liquid chromatography (UPLC) and gass chromatography-mass detection (GC-MS). Bioactive compounds were identified by spectroscopy (1H and 13C NMR) and mass spectrometric analysis. Additionally, the H. contortus eggs and infective larvae exposed to the bioactive compounds were observed through environmental scanning electron microscopy (ESEM) and confocal laser scanning microscopy (CLSM). Data were analysed based on a completely randomised design using ANOVA through a general linear model. Results The EtOAc-F fraction showed the highest ovicidal and larvicidal activities, at close to 100% at 3.12 and 6.25 mg/mL, respectively. The treatments C1F2, C1F3 and C2F3 displayed the main ovicidal activity (80–100%) at 2.5 mg/mL. The major compounds found in these sub-fractions were identified as galloyl derivatives and flavanones, including gallic acid (1), methyl gallate (2), ethyl gallate (3), naringin (4), naringenin 7-O-(4″, 6″-digalloylglucoside) (5), naringenin 7-O-(6″-galloylglucoside) (6) and naringenin (7). Likewise, the ESEM and CLSM images showed that the assessed compounds adhered to the eggshell and the external cuticle of the larvae. Conclusion These results indicate that A. farnesiana pods contain nematocidal compounds and might be promising natural anthelmintic agents against H. contortus. This leguminous plant could be used as a nutraceutical food source for the control of gastrointestinal nematodes in small ruminants.

Gastrointestinal nematodes (GIN) are responsible for enormous economic losses worldwide. The use of anthelmintic drugs reduces the parasitic burden in ruminants. However, the excessive use of these drugs triggers anthelmintic resistance in these parasites, which leads to a worrisome inefficacy of most of the commercially available antiparasitic drugs. Caesalpinia coriaria is an arboreal legume possessing medical properties, although the antiparasitic potential of this plant against animal parasitic nematodes has not yet been studied. The aim of this study was to assess the in vitro ovicidal activity of a hydro-alcoholic extract (HA-E) from C. coriaria fruits against GIN and to identify the compounds responsible for this activity through an egg hatch inhibition (EHI) assay. GIN eggs obtained from cattle faeces were used in bio-guided assays. The HA-E was subjected to a liquid-liquid extraction using water and ethyl acetate to obtain two fractions, an organic fraction (EtOAc-F, 27% yield) and an aqueous (Aq-F, 73% yield) fraction. The chromatographic fractionation of the EtOAc-F (2 gr) was performed on a glass column packed with silica gel and eluted with dichloromethane/methanol with 10% ascending polarity. The bioactive compounds were analysed using high-performance liquid chromatography (HPLC) with UV detection, nuclear magnetic resonance (NMR) spectroscopy and mass spectroscopy (MS). The HA-E extract and the EtOAc-F showed ovicidal activity at a LC50 of 0.92 and 0.16 mg/mL, respectively. A concentration-dependant effect was observed in both treatments. Chromatographic fractionation of the EtOAc-F, allowed for the isolation and characterisation of three important compounds: methyl gallate (1), gallic acid (2) and an unidentified compound (UC). The bioactive molecules (2 and UC) displayed an ovicidal activity close to 100% at 1 mg/mL concentration. The results of this work show that gallic acid (2) isolated from C. coriaria fruits is responsible for its ovicidal activity. The use of Caesalpinia coriaria could be explored in future studies as an environmentally-friendly alternative for the control of GIN in ruminants.

SUMMARYTwenty four French Alpine goats (39 ± 2·0 kg) were individually housed in a completely randomized design and fed a basal diet containing 146 g crude protein and 356 g neutral detergent fibre (NDF)/kg in the absence (control – CTRL) or presence (CELL) of 2 ml of cellulase/kg dry matter intake (DMI) for 70 days, which included a 10-day adaptation period. The feed was offered three times daily at 07·00, 13·00 and 19·00 h, but the single daily dose of cellulase was only fed at 07·00 h. Goats were hand-milked daily; milk production recorded and samples taken for compositional analysis. During the last 5 days of the experimental period, goats from each group were individually housed in stainless steel metabolic cages to enable separate and total collection of faeces and urine for nutrient digestibility and ruminal fermentation determinations. Goats fed CELL had greater DMI and greater digestibility of dry matter (DM), organic matter and NDF than CTRL goats. CELL goats had greater ruminal pH, concentration of acetic acid and concentration of propionic acid than CTRL goats. However, the concentration of ruminal butyric was lower in CELL goats compared with CTRL goats. CELL goats had greater milk yield, energy corrected milk, milk energy content, milk energy output and milk density than CTRL goats and the milk content for total solids, fat, protein and lactose were also greater for CELL goats than for the CTRL goats. The milk of CELL goats had greater palmitoleic acid, cis-10-heptadecanoic acid content and mono-saturated acids than the milk of CTRL goats and lower linoleic acid, linolenic acid contents and saturated fatty acids than the milk of CTRL goats. These results suggest that addition of 2 ml cellulase/kg DM of feed in the diet of lactating French Alpine goats elevated their milk production and improved its composition probably due to improved feed utilization.

Thirty-six goats: 15 Alpine (AG), 13 Saanen (SG) and 8 Anglo-Nubian (ANG) were used to evaluate dry matter intake (DMI), body weight (BW) changes and milk production to validate Wood's model in lactation curves. The kids pre-weaning weight, weaning weight (WW) and daily weight gain (DWG) were determined. Milk production was measured daily for a period of 90 d. The DWG was quantified daily, while BW was recorded weekly. Performance of 48 kids (20 AG, 15 SG and 13 ANG), aged 15 d were evaluated. Wood's model was utilised to describe goat's lactation curve in the different studied goat breeds. Milk production was differed between breeds and according to the number of kids suckled (P < 0.05). Both of DMI and BW showed a linear increase (P < 0.05) during the studied period. Both of WW and DWG were unaffected by type of kidding and breed and were higher in males than in females. It is suggested that equation of Wood's model is adequate to describe the lactation curve in studied goat breeds. The ANG and AG breed...

The aim of this study was to evaluate effects of free ferulic acid (FA) supplementation on pro- ductive performance, some blood metabolite concentra- tions, and carcass characteristics of ewe lambs finished in a feedlot. Dorper × Pelibuey ewe lambs (n = 20; BW = 28.5 ± 0.5 kg; age = 5 mo) were individually housed in pens and assigned under a randomized complete block design to the following dietary treatments (n = 10): daily feeding without (control) or with 300 mg of FA/ animal. The feedlot feeding period lasted 34 d and then all ewe lambs were slaughtered. Free FA did not affect (P ≥ 0.16) BW gain, ADG, DMI, and G:F during the first 17 d, but BW gain (P = 0.10) and ADG (P = 0.10) tended to decrease for FA from d 17 to 34 and from d 1 to 34 without affecting (P ≥ 0.16) DMI and G:F in ewe lambs. Serum concentrations of glucose, cholesterol, triglycer- ide, total protein, and urea were not affected (P > 0.05) by FA at d 1, 17, and 34 of the feeding period. Carcass char- acteristics were not affected (P > 0.05) by FA. Stomach percentage tended (P = 0.08) to decrease and leg yields increased (P = 0.02) for FA. Other noncarcass compo- nents and wholesale cut yields were not affected (P > 0.10) by FA. In conclusion, FA supplementation did not improve productive performance, metabolic status, and carcass characteristics of ewe lambs receiving a feedlot finishing diet.