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The diminishing nutritional quality of rice with increasing concentrations of atmospheric CO2 is currently a major global concern. The present study was designed with the objective of assessing the impact of biofertilisers on grain quality and iron homeostasis in rice under elevated CO2. A completely randomised design with four treatments ([KAU, POP (control), POP+Azolla, POP+PGPR, and POP+AMF]), each replicated three times under ambient and elevated CO2 conditions, was followed. The analysed data revealed that yield, grain quality, and iron uptake and translocation were modified in an unfavourable manner under elevated CO2, which was reflected in the lower quality and iron content of the grains. The response of iron homeostasis in the experimental plants to the application of biofertilisers, especially plant-growth-promoting rhizobacteria (PGPR), under elevated CO2 strongly suggests the possibility of utilising them for designing iron management strategies for achieving higher quality in rice.

Eco-friendly anti-algal agents are in demand for preventing the growth of unwanted algae. Green synthesized nanoparticles exhibit antimicrobial properties and have been used as a better alternative against chemical and physical processes. In the present study, treatment of silver nitrate with leaf extracts (5% w/v) of Tinospora cordifolia, a plant with proven antimicrobial effects, exhibited UV-visible absorption maxima between 440-460 nm after 1h indicating bioreduction of silver to nanoparticles. The green synthesised silver nanoparticles (5 mgl-1) exhibited inhibition zones against Chlamydomonas reinhardtii in in vitro agar assays. Treatment with green synthesised silver nanoparticles during exponential phase of algal growth resulted in significant reduction in algal population, carbohydrate, protein and chlorophyll contents confirming the anti-algal potential. This is the first report on the growth inhibitory potential of green synthesised silver nanoparticles against green algae.

Many researchers focused on green synthesis of nanomaterials, due to its simplicity, sustainability, environmental-friendly aspect and cost effectiveness. This technique, to a large extent reduce the harmful effects caused by adopting traditional and chemical methods utilized in synthesizing these nanomaterials. A two-dimensional carbon sheet – Graphene has generated huge curiosity in material researchers owed to its unique characteristics suiting varied applications. Graphene, graphene oxide and reduced graphene oxide nano sheets easily can be combined with several metals, metal oxides, nanoparticles of inorganic nature, organic polymers, semiconductors, biomolecules etc. forming a diversity of nanocomposites based on graphene for applications in power, medical therapy, sensing as well as environmental remediation. An analysis prospecting Graphene, reduced graphene oxide and solo layer graphite-based nanocomposites, numerous mechanisms of graphene-based nanocomposites (chemical along with green method), variety of utilization owned by them is presented in this paper. The photocatalytic behaviour of graphene-rooted metal, oxides of metal and oxygen group elements and its composites in nano order prepared through chemical practices and green techniques has been discussed. A remarkable improvement in photocatalytic efficiency has been reported in the composites prepared by green method which can be useful in waste water treatment, industrial and medical applications. This mini review also emphasize that at par with chemically synthesized nanocomposites of Graphene, green synthesized one has highly effective application in antibacterial, cancer therapy, along with sensing utilization. [ABSTRACT FROM AUTHOR]

The damage caused by high temperature is one of the most important abiotic stress affecting rice production. Reproductive stage of rice is highly susceptible to high temperature. The present investigation was undertaken to identify polymorphic microsatellite markers (SSR) associated with heat tolerance. The rice cultivars NERICA– L 44 (heat tolerant) and Uma (heat susceptible) were crossed to generate F1 and F2 populations. The F2 population was subjected to heat stress at >38°C and the 144 F2 plants were evaluated for their tolerance. The results note that the mean of the F2 population was influenced by the tolerant parent with regards to the traits of plant height, membrane stability index, photosynthetic rate, stomatal conductance, evapotranspiration rate, pollen viability, spikelet fertility and 1000 grain weight. Ten each of the extremely susceptible and tolerant plants were selected based on the spikelet fertility percentage. Their DNA was pooled into tolerant and susceptible bulks and Bulked Segregant Analysis (BSA) was carried out using 100 SSR markers to check for polymorphism. The survey revealed a polymorphism of 18% between the parents. RM337, RM10793, RM242, RM5749, RM6100, RM490, RM470, RM473, RM222 and RM556 are some of the prominent markers that were found to be polymorphic between the parents and the bulks. We performed gene annotation and enrichment analysis of identified polymorphic markers. Result revealed that the sequence specific site of that chromosome mostly enriched with biological processes like metabolic pathway, molecular mechanism, and subcellular function. Among that RM337 was newly reported marker for heat tolerance. Expression analysis of two genes corresponds to RM337 revealed that LOP1 (LOC_Os08g01330) was linked to high temperature tolerance in rice. The results demonstrate that BSA using SSR markers is useful in identifying genomic regions that contribute to thermotolerance.

The essential package of palliative care for cervical cancer (EPPCCC), described elsewhere, is designed to be safe and effective for preventing and relieving most suffering associated with cervical cancer and universally accessible. However, it appears that women with cervical cancer, more frequently than patients with other cancers, experience various types of suffering that are refractory to basic palliative care such as what can be provided with the EPPCCC. In particular, relief of refractory pain, vomiting because of bowel obstruction, bleeding, and psychosocial suffering may require additional expertise, medicines, or equipment. Therefore, we convened a group of experienced experts in all aspects of care for women with cervical cancer, and from countries of all income levels, to create an augmented package of palliative care for cervical cancer with which even suffering refractory to the EPPCCC often can be relieved. The package consists of medicines, radiotherapy, surgical procedures, and psycho-oncologic therapies that require advanced or specialized training. Each item in this package should be made accessible whenever the necessary resources and expertise are available.

Women with cervical cancer, especially those with advanced disease, appear to experience suffering that is more prevalent, complex, and severe than that caused by other cancers and serious illnesses, and approximately 85% live in low- and middle-income countries where palliative care is rarely accessible. To respond to the highly prevalent and extreme suffering in this vulnerable population, we convened a group of experienced experts in all aspects of care for women with cervical cancer, and from countries of all income levels, to create an essential package of palliative care for cervical cancer (EPPCCC). The EPPCCC consists of a set of interventions, medicines, simple equipment, social supports, and human resources, and is designed to be safe and effective for preventing and relieving all types of suffering associated with cervical cancer. It includes only inexpensive and readily available medicines and equipment, and its use requires only basic training. Thus, the EPPCCC can and should be made accessible everywhere, including for the rural poor. We provide guidance for integrating the EPPCCC into gynecologic and oncologic care at all levels of health care systems, and into primary care, in countries of all income levels.

PURPOSETo enable design of optimum palliative care for women with cervical cancer, we studied the most common types of suffering and their severity, prevalence, and duration.METHODSWe first reviewed the literature on the major types, severity, prevalence, and duration of suffering associated with cervical cancer. We then conducted a modified Delphi process with experts in cervical cancer care to supplement the literature. For each type of suffering, we distinguished between decedents (those who die from cervical cancer in a given year) and nondecedents (those who have cervical cancer in a given year but do not die). By applying the suffering prevalence and duration estimates to the number of decedents, nondecedents, and family caregivers in 2017, we were able to estimate their palliative care needs and the intensity of palliative care needed to respond adequately to this suffering.RESULTSThere is a high prevalence among decedents of moderate or severe pain (84%), vaginal discharge (66%), vaginal bleeding (61%), and loss of faith (31%). Among both decedents and nondecedents, there is a high prevalence of clinically significant anxiety (63% and 50%, respectively), depressed mood (52% and 38%, respectively), and sexual dysfunction (87% and 83%, respectively). Moderate or severe financial distress is prevalent among decedents, nondecedents, and family caregivers (84%, 74%, and 66%, respectively). More than 40% of decedents and nondecedents are abandoned by their intimate partners. Most patients experience some combination of moderate or severe physical, psychological, social, and spiritual suffering. In total, 258,649 decedents and 2,558,857 nondecedents needed palliative care in 2017, approximately 85% of whom were in low- and middle-income countries where palliative care is rarely accessible.CONCLUSIONAmong women with advanced cervical cancer, suffering is highly prevalent and often severe and multifaceted.

Introduction: As the pandemic of COVID-19 stretches, its wings across the different parts of the world over the past few months, it is very likely that mental health problems increase, particularly among the health-care workers who have higher risk of exposure to the disease and also to sufferings of the people affected with the disease. Objective: To assess the prevalence and factors associated with depression, anxiety, and stress among health-care workers from Kerala during the COVID-19 pandemic. Materials and Methods: A cross-sectional study among 544 health-care workers from Kerala was conducted by a self-administered online questionnaire in Google Forms by chain referral sampling with Depression, Anxiety, and Stress Scale-21 scale during initial phase of the COVID-19 pandemic. The tests of significance used were Mann–Whitney U-tests and Kruskal–Wallis tests. Odds ratios and 95% confidence interval are estimated. Results: During the early pandemic period, 9.7% of health-care workers had mild depression and 13.3% had moderate-to-severe depression. While 4% had mild anxiety and 3.5% had moderate-to-severe anxiety, about 6.8% had mild stress and 6.4% had moderate-to-severe stress. The anxiety symptoms were significantly higher among nurses compared to doctors. Emotional and social support from higher health authorities is a significant protective factor against stress and depression. Frontline workers have 84% higher risk to have depression. Conclusions: Frontline health-care workers who are directly involved in the screening, diagnosis, treatment, and care for patients with COVID-19 are at higher risk of experiencing poor mental health outcomes. Emotional and social support from higher health authorities is a significant protective factor against depression and stress among health-care workers.

High temperature induced by climatic fluctuations are an important threat for plant growth, development and quality of agricultural produces. Adaptableness to environmental changes generally derives from a large set of genetic traits affecting physio-morphological, biochemical and agronomic parameters. Therefore, the identification of genotypes with higher yield and good quality parameters at high temperatures is becoming increasingly necessary for future breeding programs. Here, we analyzed the performance of different tomato genotypes grown under elevated temperatures in terms of yield and nutritional quality of the fruit. High temperature stress was induced from flower initiation to maturity stage by keeping the pots in a temperature controlled green house facility for 45 days. The quality and yield parameters were taken at the harvesting stage. Starch and soluble sugar concentration in the leaves of tomato genotypes showed significant reduction in its amount under heat stress. Titrable acidity (TA), total soluble solids (TSS) and ascorbic acid content of tomato fruits were highest under high temperature conditions compared to ambient condition but lycopene content decreased with rise in temperature. The yield attributes viz., number of fruits/plant, fruit set %, average fruit weight (g), yield per plant (g/plant) were significantly lower for Arka Saurabh, Arka Rakshak and Pusa Rohini when compared to other genotypes under study. Molecular characterization of selected 22 tomato genotypes were assessed using 25 simple sequence repeat (SSR) markers. Phylogenetic tree was constructed by the unweighted neighbour-joining method (UPGMA) using NTSYSpc cluster analysis software. The Jaccard's similarity matrix was constructed using the SIMQUAL method using UPGMA algorithm in NTSYSpc. Jaccard's similarity matrix among these tomato genotypes ranged from a minimum of 0.22 to a maximum of 1 with an average genetic similarity of 0.67. Hence this study has importance in identifying genotypes that could maintain good quality and higher yield under high temperature condition.

Limited information exists regarding the ideal packaging and storage conditions for optimum quality and storage life of rambutan. In this study, the physicochemical properties of rambutan fruits were monitored during storage. Fruit were packaged in Ventilated LDPE (150 gauge), Ventilated Polypropylene (PP) (100 gauge), Polystyrene tray with cling film wrap, Shrink wrapping and stored under room (30±2?C and RH 80-85%) or refrigerated (10±2?C and RH 90-95%) conditions were investigated for their potential to enhance the quality and extend the storage life of the fruit. Rambutan pre-packaged in ventilated polypropylene (PP) reduced weight loss. Refrigerated storage extended storage life of PP packed fruits compared to un-refrigerated fruits up to 14 days. Result of study revealed that Rambutan pre-packaging of rambutan in ventilated polypropylene (PP) reduced weight loss, while refrigerated storage extended storage life of PP packed fruits compared to un-refrigerated fruits up to 14 days. These results provide important information regarding the storage behavior of rambutan fruits and indicate that packaging and storage conditions are effective for reducing moisture loss and extending storage life of fresh rambutan fruit.

A study was undertaken to evaluate the physiological and biochemical parameters and to quantify yield of twenty-two contrasting tomato genotypes when exposed to high temperature stress. Experiment was conducted in the Department of Plant Physiology, College of Agriculture, Vellayani during two growing seasons i.e., September to December, 2019 and March to June, 2020. The experiment was laid out in completely randomized design with two treatment levels i.e. control and high temperature stress (36+/-2oC) with three replications each under first season and 38+/-2oC during the second season. The high temperature stress was induced from flower initiation to maturity stage by keeping the pots in a temperature controlled green house facility for 45 days. The presence of stigma exertion, reduction in chlorophyll content and photosynthetic rate, reduced starch content and soluble sugar content, reduced chlorophyll fluorescence, low leaf membrane thermostabilty (LMT) were the major limitations for reproductive success and good yield. The correlation and regression analysis showed that yield under control conditions had strong positive correlation between LMT, pollen viability, photosynthetic rate, chlorophyll fluorescence, starch, soluble sugar and chlorophyll content. A negative correlation was observed between yield and stomatal conductance and transpiration rate. Under high temperature stress condition, all the parameters showed a positive correlation with yield. Based on the performances of various tomato genotypes under control and heat stress conditions, we were able to identify traits that could be manipulated to improve the high temperature tolerance in tomato. [ABSTRACT FROM AUTHOR]

Background: In brick kiln and construction industry the exposure to carbon monoxide and silica dust is the most common occupational hazard to the workers in these industries. A study on occupational health hazards of working women in these two unorganized sectors was undertaken by Lokmanya Medical Research Centre. Objectives: To study the effect of work site environment on the health of the women working in brick kiln and construction industry. An attempt was also made to study the seasonal changes in the concentration of carbon monoxide and dust at the worksite. Material and Methods: A cross-sectional study was conducted among the working women (age 18-40 years) at brick kilns and construction sites during summer and winter season. They were examined primarily to assess the effect of working environment on health. Gasteck Detector Pump of model 800 and air sampling instrument (SKC Air Check–52) were used to measure concentration of carbon monoxide and dust in the air respectively. Results: There were 66% of women who were in the age group of 18-40 years and most of them (94%) were married. At brick kiln sites, average CO exposure was 62.8 ppm and 55.5 ppm and average dust exposure was 3 3 146.1 mg/m and 91.4 mg/m in summer and winter season respectively.At construction sites, average dust exposure was 41.5 ppm and 90.8 ppm in summer and winter. Conclusion: Both exposure to CO and dust were more in summer than in winter in brick kiln industry whereas in construction industry the exposure to dust was more in winter season. A high level of morbidity in the form of headache, bodyache, problems with vision, cough and breathlessness were observed in both industries. It is strongly recommended to take pollution control measures.