1. Tip chip: Subcellular sampling from single cancer cells
- Author
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Quist, Jos, Sarajlic, Edin, Lai, Stanley C.S., Lemay, Serge G., and Bio electronics
- Subjects
Single-cell analysis ,Isotachophoresis ,Fluidic force microscopy ,Subcellular sampling - Abstract
To analyze the molecular content of single cells, cell lysis is typically required, yielding a snapshot of cell behavior only. To follow complex molecular profiles over time, subcellular sampling methods potentially can be used, but to date these methods involve laborious offline analysis. Here we report a "tip chip" device for continuous online coupling of subcellular sampling and on-chip sample analysis. The device contains a hollow, sharp, out-of-plane tip connected to a microfluidic channel structure. After positioning a cell onto the tip using a fluidic force microscope, intracellular molecules diffuse into the microchannel and are directly analyzed using isotachophoresis.
- Published
- 2016