596 results on '"Quillaja"'
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2. Safety and efficacy of a feed additive consisting of Quillaja saponaria Molina and Yucca schidigera Roezl ex Ortgies (Magni‐PHI®) for all poultry species (to slaughter age/weight, or to the point of lay) and ornamental birds (Phibro Animal Health Corporation)
- Author
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EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP), Vasileios Bampidis, Giovanna Azimonti, Maria de Lourdes Bastos, Henrik Christensen, Mojca Durjava, Birgit Dusemund, Maryline Kouba, Marta López‐Alonso, Secundino López Puente, Francesca Marcon, Baltasar Mayo, Alena Pechová, Mariana Petkova, Fernando Ramos, Roberto Edoardo Villa, Ruud Woutersen, Paul Brantom, Andrew Chesson, Johannes Westendorf, Jaume Galobart, Jordi Ortuño, Paola Manini, Jordi Tarrés‐Call, Maria Vittoria Vettori, and Fabiola Pizzo
- Subjects
all avian species ,digestibility enhancers ,efficacy ,Quillaja ,safety ,Yucca ,Nutrition. Foods and food supply ,TX341-641 ,Chemical technology ,TP1-1185 - Abstract
Abstract Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of a feed additive consisting of Quillaja saponaria powder and Yucca schidigera powder (Magni‐Phi®) for all avian species (to slaughter age/weight, or to the point of lay) and ornamental birds, as a zootechnical additive (digestibility enhancer and other zootechnical additives). The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the additive is safe for chickens for fattening at the level of 250 mg/kg complete feed with a margin of safety of 20 assuming that the additive contains 3.58% of saponins. This conclusion was extrapolated to all growing poultry species and ornamental birds. The Panel concluded that the use of the feed additive in animal nutrition at 250 mg/kg complete feed is of no concern for the safety for the consumer and the environment. The Panel also concluded that the additive is not irritant to skin, but irritant to the eyes and to the respiratory system. Due to the lack of data, the FEEDAP Panel could not conclude on the skin sensitisation potential of the additive. The FEEDAP Panel was not in the position to conclude on the efficacy of the additive for all poultry species and ornamental birds.
- Published
- 2024
- Full Text
- View/download PDF
3. Applications of Saponin Extract from Asparagus Roots as Functional Ingredient.
- Author
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Hamdi, Amel, Viera-Alcaide, Isabel, Jiménez-Araujo, Ana, Rodríguez-Arcos, Rocío, and Guillén-Bejarano, Rafael
- Subjects
SAPONINS ,ASPARAGUS ,SURFACE active agents ,MYCOSES ,ROOT crops ,LIPASES - Abstract
When replanting an asparagus field, the roots of the previous crop are crushed and incorporated into the soil, creating problems of autotoxicity and fungal infections. Asparagus roots can be considered as a valuable byproduct, since they are very rich in saponins (3–6%), compounds currently considered as bio-emulsifiers. The objective is to evaluate the emulsifying and foaming capacity of a saponin extract from asparagus roots (ARS) and compare it with other commercial extracts. ARS was obtained using a process patented by our research group. The results have shown that ARS has activity similar to Quillaja extract. Its critical micellar concentration falls between that of Quillaja and Tribulus extracts (0.064, 0.043, and 0.094 g/100 mL, respectively). Both emulsifying and foaming activities are affected by pH, salt, and sucrose to a similar extent as the other extracts. Additionally, it has demonstrated an inhibitory effect on pancreatic lipase, which is even better than the other two studied extracts, as indicated by its IC
50 value (0.7887, 1.6366, and 2.0107 mg/mL for asparagus, Quillaja, and Tribulus, respectively). These results suggest that ARS could serve as a natural emulsifying/foaming agent for healthier and safer food products and as a potential aid in treatments for obesity and hyperlipidemia. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
4. Synthesis of silver nanoparticles using Quillaja saponaria Molina bark extract and its antivenom activities.
- Author
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Parua, Poulami, Saha, Kanchan, Sarkhel, Sumana, Chatterjee, Upasana, Jamal, Nuzhat Ara, and Pradhan, Simi Manna
- Subjects
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SILVER nanoparticles , *QUILLAJA , *ANTIVENINS , *SCANNING electron microscopy , *X-ray diffraction - Abstract
Snakebite is an issue of concern, especially in India which accounts for half of the global deaths due to venomous snakebites every year. Saponins are glycosides of triterpenes and steroids known for their antivenom properties. The soap bark tree or Quillaya, Quillaja saponaria Molina represents the major resource of saponins for commercial applications. Here, we investigated the antivenom property of silver nanoparticle mediated saponins (AgNP-SP) from the Q. saponaria bark extract. AgNP-SP was prepared conducive to optimal temperature, pH of extract and concentration of AgNO3. UV-VIS, FT-IR, XRD, TEM and SEM interpretations were devised to characterize AgNP-SP. AgNP-SP was tested for its efficacy to neutralize venom lethality and increase in myotoxicity biomarkers (LDH) in animal models. AgNP-SP was synthesized optimally at a concentration of 50 mg/mL, extract (pH 6.8) and temperature (80°C) with AgNO3 (1 mM). The colour change and synthesis of AgNP-SP was examined by UV-Vis analysis at 430 nm. TEM studies showed the size for AgNP-SP to be 74.4 nm. FT-IR analysis showed peaks of AgNP-SP at 3422 cm-1 and 2926 (O-H stretching), 2358 cm-1 (O=C=O stretching), 1616 cm-1 (C=C stretching), 1097 cm-1 (C-F stretching), 813 cm-1 (C-CL stretching) and 651 cm-1 (C-Br stretching). The EDAX established the purity of the AgNP-SP. The biosynthesized AgNP-SP could significantly neutralize Vipera russelli venom (VRV) mediated elevation of biomarkers lactate dehydrogenase (LDH), serum glutamate pyruvate transaminase (SGPT) serum glutamic-oxaloacetic transaminase (SGOT), serum creatinine and serum uric acid. The present study, thus promulgates the therapeutic potential of silver nanoparticle mediated saponins (AgNP-SP) in ameliorating the biochemical and pharmacological effect of haemotoxic snake venom in animal models. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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5. Surface-active natural saponins. Properties, safety, and efficacy.
- Author
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Fink, Rok and Filip, Sebastjan
- Subjects
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DRUG efficacy , *ESCHERICHIA coli , *GLYCOSIDES , *BIOFILMS , *SURFACE active agents , *ENVIRONMENTAL health , *QUILLAJA , *SURFACE properties , *ANTIBIOTICS , *BACTERIA , *PHARMACODYNAMICS - Abstract
In the future, cleaning products must fulfil the principles of green chemistry while maintaining efficacy against bacteria. This study aims to evaluate the detergent properties, ecotoxicity, and anti-biofilm potential of natural saponins compared to synthetic surfactants. We tested sodium dodecyl sulphate, quillaja saponin, escin, and sapogenin for emulsifying capacity, critical micelle concentration, ecotoxicity to yeast, and antibacterial and anti-biofilm potential against bacteria. The results show that the emulsifying capacities of quillaja saponin and sodium dodecyl sulphate are similar, while the critical micelle concentration for quillaja saponin is much lower. Furthermore, the antibacterial and antibiofilm potentials are much higher for quillaja saponin than for synthetic sodium dodecyl sulphate. Moreover, we have shown that natural saponins are less toxic to the S. cerevisiae than synthetic saponin is. All these facts indicate that quillaja is a suitable candidate to replace synthetic products as it meets the requirements of efficacy and safety. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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6. Applications of Saponin Extract from Asparagus Roots as Functional Ingredient
- Author
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Amel Hamdi, Isabel Viera-Alcaide, Ana Jiménez-Araujo, Rocío Rodríguez-Arcos, and Rafael Guillén-Bejarano
- Subjects
bio-emulgent ,foaming properties ,saponins ,steroid ,Quillaja ,asparagus root ,Chemical technology ,TP1-1185 - Abstract
When replanting an asparagus field, the roots of the previous crop are crushed and incorporated into the soil, creating problems of autotoxicity and fungal infections. Asparagus roots can be considered as a valuable byproduct, since they are very rich in saponins (3–6%), compounds currently considered as bio-emulsifiers. The objective is to evaluate the emulsifying and foaming capacity of a saponin extract from asparagus roots (ARS) and compare it with other commercial extracts. ARS was obtained using a process patented by our research group. The results have shown that ARS has activity similar to Quillaja extract. Its critical micellar concentration falls between that of Quillaja and Tribulus extracts (0.064, 0.043, and 0.094 g/100 mL, respectively). Both emulsifying and foaming activities are affected by pH, salt, and sucrose to a similar extent as the other extracts. Additionally, it has demonstrated an inhibitory effect on pancreatic lipase, which is even better than the other two studied extracts, as indicated by its IC50 value (0.7887, 1.6366, and 2.0107 mg/mL for asparagus, Quillaja, and Tribulus, respectively). These results suggest that ARS could serve as a natural emulsifying/foaming agent for healthier and safer food products and as a potential aid in treatments for obesity and hyperlipidemia.
- Published
- 2024
- Full Text
- View/download PDF
7. Elucidation of the pathway for biosynthesis of saponin adjuvants from the soapbark tree.
- Author
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Reed, James, Orme, Anastasia, El-Demerdash, Amr, Owen, Charlotte, Martin, Laetitia B. B., Misra, Rajesh C., Kikuchi, Shingo, Rejzek, Martin, Martin, Azahara C., Harkess, Alex, Leebens-Mack, Jim, Louveau, Thomas, Stephenson, Michael J., and Osbourn, Anne
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SAPONINS , *QUILLAJA , *BIOSYNTHESIS , *VACCINES , *ENZYMES - Abstract
The Chilean soapbark tree (Quillaja saponaria) produces soap-like molecules called QS saponins that are important vaccine adjuvants. These highly valuable compounds are sourced by extraction from the bark, and their biosynthetic pathway is unknown. Here, we sequenced the Q. saponaria genome. Through genome mining and combinatorial expression in tobacco, we identified 16 pathway enzymes that together enable the production of advanced QS pathway intermediates that represent a bridgehead for adjuvant bioengineering. We further identified the enzymes needed to make QS-7, a saponin with excellent therapeutic properties and low toxicity that is present in low abundance in Q. saponaria bark extract. Our results enable the production of Q. saponaria vaccine adjuvants in tobacco and open the way for new routes to access and engineer natural and new-to-nature immunostimulants. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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8. Liquid Chromatography-Electrospray Ionization Mass Spectrometry Analysis of Quil-A: an Aqueous Extract from the Bark of Quillaja saponaria Molina.
- Author
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Tebogo, Moshegwana O., Monkgogi, Thatayaone, Moshapa, Florah, Rapaka, Deepthi, Bitra, Veera R., and Adiukwu, Paul C.
- Subjects
QUILLAJA ,LIQUID chromatography ,SAPONINS ,DATA libraries ,ACETONITRILE - Abstract
Quil-A, a commercial product of the aqueous extract of Quillaja saponaria Molina bark, indigenous to Chile has been widely reported for its immuno-modulatory and adjuvant properties. The saponin constituent of the plant extract, which is responsible for these properties, is commonly separated using liquid chromatography techniques. However, the application of this technique is impaired by the non-saponin constituents of the aqueous matrix, leading to the shortening of the column life. Therefore, the study was to develop a reverse-phase liquid chromatography technique to improve the resolution of the aqueous matrix. Applying the reverse phase liquid chromatography using optimized gradient solvent mixtures of water, acetonitrile, and 10 mM ammonium acetate, components from the sample were eluted on a C5 reverse phase column. Liquid chromatography peaks at 214 nm, and electrospray ionization mass spectrometer signals were identified as per the NIST-MS data library. The finding indicates the presence of ions of molecules previously reported. Also, ions of high molecular weight saponin-like molecules, which are yet to be reported, were eluted at early retention times of the chromatography elution. The study suggests the possible presence of molecules, which could be novel and contribute to the ultimate benefits of Quil-A. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
9. Effect of emulsifiers on the discoloration of chlorophyll and their potential for use in green beverages.
- Author
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Yasuda, Midori and Tabata, Masaaki
- Subjects
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CHLOROPHYLL , *DISCOLORATION , *BEVERAGES , *SUCROSE esters , *FATTY acid esters , *QUILLAJA - Abstract
The discoloration of chlorophyll (Chl) by light is an ongoing issue for green beverages in the food industry. To suppress the discoloration of Chl in aqueous solution, the effects of different emulsifiers were investigated on the discoloration of Chl under ultraviolet (UV) irradiation to determine their potential application for use as food additives. Sucrose fatty acid ester (SE), sorbitan fatty acid ester (TW), and quillaja saponin (QS) were used as emulsifiers, while Triton X‐100 (TX) was used for reference. The discoloration of Chl was measured using a color difference meter. The species of Chl in solution were determined using ultraviolet–visible (UV–Vis), fluorescence, and circular dichroism (CD) spectroscopy, and the particle size of Chl in solution was determined using dynamic light scattering. The Chl aggregates were observed by the observation of increased peak areas at longer wavelengths in the UV spectra of Chl, in addition to a reduced fluorescence intensity. The CD spectra showed that the Chl aggregates were arranged in a random structure. Furthermore, the average particle size of the Chl aggregates was determined to be approximately 100 nm. SE and QS were found to significantly enhance the formation of self‐aggregates due to their high hydrophilicities compared to those of TW and TX. As a result, SE and QS protect themselves from light to suppress the discoloration of Chl. The present results therefore suggest that SE and QS are suitable emulsifiers to address the problem of Chl discoloration in beverages, such as green tea and vegetable juices. Practical Application: Chlorophyll (Chl), a green pigment present in vegetables and green tea, is discolored by light. In this study, it was found that emulsifiers (sucrose fatty acid ester and quillaja saponin) suppress the discoloration of Chl. The implementation of these emulsifiers as food additives would enable green tea or green vegetable juices to maintain their colors for long periods and could contribute significantly to the beverage industry. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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10. The Effects of a Combination of Quillaja saponaria and Yucca schidigera on Eimeria spp. in Broiler Chickens.
- Author
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Bafundo, K. W., Johnson, A. B., and Mathis, G. F.
- Subjects
BROILER chickens ,EIMERIA ,TRITERPENOID saponins ,EIMERIA tenella ,AVIAN coccidiosis - Abstract
Copyright of Avian Diseases is the property of American Association of Avian Pathologists, Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2020
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11. In vitro evaluation and molecular docking of QS-21 and quillaic acid from Quillaja saponaria Molina as gastric cancer agents.
- Author
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Guzmán, Leda, Villalón, Katherine, Marchant, María José, Tarnok, María Elena, Cárdenas, Pilar, Aquea, Gisela, Acevedo, Waldo, Padilla, Leandro, Bernal, Giuliano, Molinari, Aurora, and Corvalán, Alejandro
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QUILLAJA , *MOLECULAR docking , *SAPONINS , *ANTINEOPLASTIC agents , *IN vitro studies - Abstract
The cytotoxic mechanism of the saponin QS-21 and its aglycone quillaic acid (QA) was studied on human gastric cancer cells (SNU1 and KATO III). Both compounds showed in vitro cytotoxic activity with IC50 values: 7.1 μM (QS-21) and 13.6 μM (QA) on SNU1 cells; 7.4 μM (QS-21) and 67 μM (QA) on KATO III cells. QS-21 and QA induce apoptosis on SNU1 and KATO III, as demonstrated by TUNEL, Annexin-V and Caspase Assays. Additionally, we performed in silico docking studies simulating the binding of both triterpenic compounds to key proteins involved in apoptotic pathways. The binding energies (∆Gbin) thus calculated, suggest that the pro-apoptotic protein Bid might be a plausible target involved in the apoptotic effect of both triterpenic compounds. Although QA shows some antiproliferative effects on SNU1 cells cultured in vitro, our results suggest that QS-21 is a more powerful antitumor agent, which merits further investigation regarding their properties as potential therapeutic agents for gastric cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
12. Formation and stability of emulsions stabilized by Quillaja saponin–egg lecithin mixtures.
- Author
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Salminen, Hanna, Bischoff, Sonja, and Weiss, Jochen
- Subjects
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EMULSIONS , *QUILLAJA , *LECITHIN , *SAPONINS , *STABILIZING agents - Abstract
Knowledge of binary emulsifiers' influence on the formation and stability of emulsion‐based products is still limited. The aim of this study was to investigate the emulsifying properties of Quillaja saponin–egg lecithin mixtures at different concentration ratios (r = 5:0, 4:1, 3:2, 2:3, 1:4, and 0:5) with total emulsifier concentration set to 0.5% or 1.0% (w/w). For this, oil‐in‐water emulsions (10% oil, pH 7) were prepared via high‐pressure homogenization. Furthermore, emulsion stability against different environmental stresses was tested. All the binary emulsifier mixtures formed submicron sized emulsions upon homogenization. The most stable emulsions among the mixed emulsifiers were obtained at low Quillaja saponin concentration at r = 1:4 that showed similar physical stability over time to emulsions stabilized by Quillaja saponins and egg lecithin alone. The data suggested that the mixtures of Quillaja saponins and egg lecithins built mixed interfacial layers that were prone to changes over time. Emulsions stabilized by the binary mixtures were in general less stable against changes in pH and ionic strength than the emulsions stabilized by the individual emulsifiers. An exception were the emulsions at r = 1:4 that showed improved stability at pH 2 over the phase separated Quillaja saponin–stabilized emulsions at the same pH. Moreover, all the emulsions were heat stable up to 90 °C. On the other hand, none of the emulsions were stable upon freeze‐thawing. These results increase our understanding of technofunctionality of binary emulsifier systems. Practical Application: Food‐grade and natural emulsifier mixtures composed of Quillaja saponins and egg lecithin may be used in selected emulsion‐based food or personal care product applications to replace synthetic surfactants due to issues with consumer acceptance and regulatory restrictions. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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13. A booster for vaccines from plants.
- Author
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Nunes, Helena H. Chubatsu and Dang, Thu-Thuy T.
- Subjects
- *
QUILLAJA , *VACCINE immunogenicity , *TRITERPENOID saponins , *SUSTAINABILITY , *CHEMICAL synthesis , *IMMUNE response - Abstract
The article discusses QS-7, a promising vaccine adjuvant derived from the rare Chilean soapbark tree or Quillaja saponaria. The QS adjuvants belong to a group of secondary metabolites called triterpenoid glycosides or saponins. Due to the complexity of the QS structures, which has challenged traditional chemical synthesis, understanding QS biosynthesis in the native tree presents an opportunity to obtain these pharmaceuticals sustainably.
- Published
- 2023
- Full Text
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14. Little Vaccine Essentials.
- Author
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Subramanian, Samanth
- Subjects
COVID-19 vaccines ,MEDICAL supply manufacturing ,BOROSILICATES ,VIALS ,HYPODERMIC syringes ,QUILLAJA - Abstract
The article reports on the future demand for ancillary medical supplies for the distribution of a COVID-19 vaccine. It mentions the need for medical-grade borosilicate glass to make vials for vaccines, soapbark for vaccine adjuvants, and hypodermic syringes to deliver the vaccines.
- Published
- 2020
15. Enhancing the formation and stability of emulsions using mixed natural emulsifiers: Hydrolyzed rice glutelin and quillaja saponin.
- Author
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Xu, Xingfeng, Sun, Qingjie, and McClements, David Julian
- Subjects
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QUILLAJA , *SAPONINS , *EMULSIONS , *MEDICINAL plants , *STABILIZING agents - Abstract
Abstract Emulsifiers can often be used in combination to improve their functional performance. In this study, we examined the potential of combining two natural emulsifiers, hydrolyzed rice glutelin (HRG) and quillaja saponin (QS), on the formation and stability of oil-in-water emulsions. In particular, we investigated the impact of total emulsifier level, QS-to-HRG ratio, pH, ionic strength, and temperature on the mean particle diameter (d 3,2), ξ-potential, creaming stability, and microstructure of the emulsions. The size of the oil droplets produced by homogenization decreased as the QS-to-HRG ratio increased from 1:4 to 1:1, but remained constant after that. The ξ-potential and dimensions of the droplets coated by the mixed emulsifiers were reasonably similar to those coated by QS alone, suggesting that the adsorbed saponins dominated the interfacial properties in the mixed systems. We also determined the physical stability of emulsions prepared using HRG, QS, or 1:1 QS-HRG after exposure to pH changes (pH 3–7), salt addition (0–500 mM NaCl, pH 7) and thermal treatment (30–90 °C 30 min, 200 mM NaCl, pH 7). QS-emulsions were stable to aggregation and creaming over a wider pH range than the other emulsions. Conversely, the mixed emulsions had better stability to high salt levels and temperatures (pH 7), which was attributed to a thicker interfacial layer causing stronger steric repulsion. This research may increase the utilization of plant-based emulsifiers in food and beverage applications. Graphical abstract Image 1 Highlights • Hydrolyzed rice glutelin (HRG) and quillaja saponin (QS) were used to form emulsions. • These plant-based emulsifiers had synergistic effects on improving emulsion stability. • The mixed emulsifiers (1:1) gave improved stability at high ionic strengths and temperatures. • These plant-based emulsifiers may be suitable for application in foods and beverages. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
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16. Effects of Quillaja saponin supplementation on growth performance, nutrient digestibility, fecal microbiota, and fecal gas emission in growing pigs
- Author
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In Ho Kim and De Xin Dang
- Subjects
chemistry.chemical_classification ,Nutrient digestibility ,Saponin ,Biology ,Fecal microbiota ,Body weight ,biology.organism_classification ,Crossbreed ,Fecal coliform ,Animal science ,Food Animals ,chemistry ,Quillaja ,Animal Science and Zoology ,Feces - Abstract
The purpose of this study was to evaluate the effects of dietary supplementation of Quillaja saponin (QS) on growth performance, nutrient digestibility, fecal microbiota, and fecal gas emission in growing pigs. A total of 50 crossbred growing pigs [(Yorkshire × Landrace) × Duroc] with an initial body weight of 23.83 ± 1.95 kg were randomly assigned to one of two treatments for a 56 d trial with five replicate pens per treatment and five pigs (two barrows and three gilts) per pen. Dietary treatments including control diet and control diet supplemented with 200 mg·kg−1 QS. The average daily gain was significantly increased during days 0–56, whereas the fecal ammonia emission on day 56 and fecal coliform bacteria counts on day 28 were significantly decreased in pigs fed with QS-containing diet. However, dietary supplementation of QS had no significant effects on apparent total tract digestibility. In conclusion, dietary supplementation of 200 mg·kg−1 QS had beneficial effects on growth performance, fecal microbiota, and fecal gas emission in growing pigs. Considering the carry-over effects, the adaption period should be at least 28 d when supplementing 200 mg·kg−1 QS to the diet of growing pigs for improving the growth performance.
- Published
- 2021
17. Quantitation of Mi-saponin A in adulterated Quillaja and contaminated Gypsophila saponin extracts by high performance liquid chromatography/quadrupole time-of-flight mass spectrometry
- Author
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Wolfgang Buchberger, Bernhard Thalhamer, Markus Himmelsbach, and Victoria Haider
- Subjects
chemistry.chemical_classification ,Residue (complex analysis) ,Chromatography ,Gypsophila ,biology ,Saponin ,Madhuca longifolia ,Plant Science ,Madhuca ,biology.organism_classification ,Biochemistry ,High-performance liquid chromatography ,Ingredient ,chemistry ,Quillaja ,Agronomy and Crop Science ,Biotechnology - Abstract
Plant saponin extracts are currently of increasing importance for the environment and climate. Saponin extracts are used for the regeneration of soils contaminated by environmental pollutants such as polycyclic aromatic hydrocarbons. Furthermore, as feed additives they may have the potential to reduce the release of the greenhouse gas methane in ruminants, or to reduce ammonia emissions in pigs and broilers. Quillaja extracts play a major role in emission reduction. Some commercially available Quillaja extracts have been adulterated by blending with an inexpensive Madhuca saponins extract. Since a quantitation has not been performed so far, the present work aims at the development of a quantitative method for the determination of the main ingredient Mi-saponin A of Madhuca. For this purpose, a semi-preparative HPLC method for the isolation of Mi-saponin A from mahua seed cake, which is the press residue of the seed kernels of Madhuca longifolia, was developed. Using 1H NMR, 13C NMR, and high resolution mass spectrometry, the identity was unambiguously confirmed and the purity was excellent. It was used as standard for quantitation of Mi-saponin A in various currently purchased commercially available Quillaja saponin extracts. The concentration of Mi-saponin A was between 1.68 and 2.65 % (w/w), which reveals that the blending of Quillaja saponin extracts with Madhuca is still relevant. Moreover, the developed method for the quantitation of Mi-saponin A is also suitable for lower concentration. Thus, Mi-saponin A was found at a concentration of 0.10 % (w/w) in Gypsophila saponin extracts, confirming that there was a contamination with this Madhuca saponin.
- Published
- 2021
18. Emulsification by vitamin E TPGS or Quillaja extract enhanced absorption of berberine without affecting its metabolism in humans
- Author
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Yavuz Yagiz, Gary P. Wang, and Liwei Gu
- Subjects
Male ,Cross-Over Studies ,Berberine ,Plant Extracts ,Tandem Mass Spectrometry ,Quillaja ,Humans ,Vitamin E ,Female ,General Medicine ,Powders ,Food Science - Abstract
Berberine is widely used for the prevention of cancers and diabetes. However, the absorption rate of berberine is less than 1% in humans. The objective of this research was to determine whether emulsification improves the absorption and affects the metabolism of orally ingested berberine. Twelve healthy subjects, both men and women, received 800 mg berberine in a powder or emulsified form by vitamin E TPGS or Quillaja extract using a randomized crossover design. Blood samples were collected 12 hours after a dose. Berberine and its metabolites in plasma were analyzed with and without hydrolysis by glucuronidase and sulfatase on UHPLC-MS/MS. The area under the curve (AUC
- Published
- 2022
19. Natural antimicrobial delivery systems: Formulation, antimicrobial activity, and mechanism of action of quillaja saponin-stabilized carvacrol nanoemulsions.
- Author
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Ryu, Victor, McClements, David J., Corradini, Maria G., Yang, Jason Szuhao, and McLandsborough, Lynne
- Subjects
- *
QUILLAJA , *CARVACROL , *EMULSIONS , *ANTI-infective agents , *DRUG delivery systems , *BIOCHEMICAL mechanism of action - Abstract
The objective of this research was to develop antimicrobial nanoemulsions using quillaja saponin as a natural surfactant, carvacrol as an antimicrobial agent, and medium chain triglyceride (MCT) oil as a ripening inhibitor. Oil-in-water antimicrobial nanoemulsions were fabricated by homogenizing 10 wt% oil phase (carvacrol and MCT) with 90 wt% aqueous phase (quillaja saponin in 5 mM sodium citrate buffer, pH 3.5). The physical stability and antimicrobial activity of these systems was compared to nanoemulsions formed using a synthetic non-ionic surfactant (Tween 80). Stable nanoemulsions with small mean droplet diameters ( d < 150 nm) could be formed by optimizing oil phase composition and surfactant level. The antimicrobial activity of carvacrol nanoemulsions formulated with Q-Naturale ® was higher than that formulated with Tween 80. Dialysis and chromatography were used to monitor the transfer of carvacrol from one hydrophobic phase to another, to mimic transport from oil droplets to bacterial cell membranes. The antimicrobial activity depended on the concentration and type of surfactant used and the ability of surfactant micelles to transfer carvacrol through the aqueous phase. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
20. Effect of saponins of Quillaja saponaria extracts in combination with Pseudomonas protegens to control Gaeumannomyces graminis var. tritici in wheat.
- Author
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González-Castillo, Jonathan A., Quezada-D'Angelo, Tamara P., Silva-Aguayo, Gonzalo I., and Moya-Elizondo, Ernesto A.
- Subjects
- *
SAPONINS , *QUILLAJA , *PSEUDOMONAS , *WHEAT farming , *PLANT diseases - Abstract
Extracts rich in saponins from Quillaja saponaria Mol. and populations of rhizobacteria from the genus Pseudomonas, which produce antimicrobial compounds, have been associated with reduction of the fungus Gaeumannomyces graminis var. tritici, the causal agent of take-all disease, which is responsible for severe loss of wheat (Triticum aestivum L.) crops worldwide. However, there is a limited background on the interaction between these bacteria and natural triterpenoids. The aim of this research was to assess the effect of saponin rich extract on Pseudomonas protegens strains 2,4-diacetylphloroglucinol-producers under in vitro and in plant conditions and determining the synergistic effect to be used together to control G. graminis var. tritici in wheat plants. We determined that 8% and 90% of saponins rich Q. saponaria extracts have a differential effect on P. protegens according to their purity (P ≤ 0.05). On wheat seedlings, quillaja extract with 90% of saponins did not affect the three antagonistic bacterial strain populations assessed, but affected biofilm formation at saponins concentration of 7360 mg L-1. Pseudomonas protegens strains had a variable antagonist activity in wheat plants, and controlled the fungus when were combined with different concentrations of pure Q. saponaria extract, with the concentration of 1840 ppm reducing the take-all disease in 32.5% with respect to the control inoculated with G. graminis var. tritici (P ≤ 0.05). However, no synergistic effects when the plant extract was combined with the bacterial strains were observed. These results showed the promising and complexity of combining bacterial and plant extract to develop a biopesticide, which could control this fungal disease. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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- View/download PDF
21. Simultaneous determination of six bioactive saponins from Rhizoma Panacis Japonici in rat plasma by UHPLC-MS/MS: Application to a pharmacokinetic study.
- Author
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Zheng, Hong, Qiu, Feng, Zhao, Hui, Chen, Jie, Wang, Lei, and Zou, Haiyan
- Subjects
- *
SAPONINS , *PHARMACOKINETICS , *GINSENOSIDES , *ELECTROSPRAY ionization mass spectrometry , *FORMIC acid , *GLUCOSIDES , *QUILLAJA - Abstract
A specific, sensitive and rapid ultra high performance liquid chromatography-tandem mass spectrometric (UHPLC-MS/MS) method was developed and validated for simultaneous determination of six major bioactive constituents in Rhizoma Panacis Japonici (RPJ), including oleanolic acid-type chikusetsusaponin V, IV, hemsgiganoside B, damarane-type ginsenoside Rb1, Rg1 and Re in rat plasma, using estazolam as the internal standard (IS). Plasma samples were pretreated with methanol/acetonitrile (1:1, V/V) for protein precipitation. Chromatographic separation was performed on an Agilent Eclipse Plus C 18 column, using a gradient mobile phase consisting of methanol and 0.1% formic acid aqueous solution. A tandem mass spectrometric detection with an electrospray ionization (ESI) interface was conducted via multiple reaction monitoring (MRM) under positive ionization mode. For all the six analytes of interest, the calibration curves were linear in the concentration range of 2.00–500 ng/mL with r ≥ 0.9956. The intra- and inter-day precisions (in terms of relative standard deviation, RSD) were all below 10.2% and the accuracies (in terms of relative error, RE) were within −5.0% to 6.3% for all six analytes. Extraction recovery, matrix effect and stability data all met the acceptance criteria of FDA guideline for bioanalytical method validation. The developed method was applied to the pharmacokinetic study in rat. After oral administration of the total saponins from RPJ, six analytes were quickly absorbed into the blood and presented the phenomenon of double peaks. Among the six analytes, ginsenoside Rb1 showed slowest elimination from plasma with a t 1/2z of 16.00 h, while that of the others were between 1.72 and 5.62 h. In conclusion, the developed method was successfully used to simultaneously analyze major oleanolic acid-type and damarane-type saponins of RPJ in rat plasma after oral administration. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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- View/download PDF
22. Fabrication of Origanum compactum essential oil nanoemulsions stabilized using Quillaja Saponin biosurfactant.
- Author
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Sedaghat Doost, Ali, Devlieghere, Frank, Dirckx, Ann, and Van der Meeren, Paul
- Subjects
- *
FABRICATION (Manufacturing) , *ORIGANUM , *ESSENTIAL oils , *QUILLAJA , *BIOSURFACTANTS - Abstract
Abstract: There is an increased interest for essential oil nanoemulsions as natural food preservatives. We utilized sugar‐based surfactants including Quillaja Saponin (QS), Sucrose Monopalmitate (SMP), and Octyl Modified Starch (OMS). The outcomes presented that nanoemulsions could be successfully formed. Whereas oregano emulsions stabilized by SMP exposed to salt and acidic pH were highly unstable, QS could form more stable colloidal systems. Oregano nanoemulsions containing a very low amount of sunflower oil (SO) were not susceptible to Ostwald ripening during 60 days of storage at 20 °C. The optimization of the production process through response surface methodology revealed that incorporation of only 6.9 wt % SO at a microfluidization pressure of 73.5 MPa was sufficient to form a long‐term stable nanoemulsion with no changes in antibacterial activity. This work gives valuable and practical information to produce a natural antimicrobial agent potentially applicable in food and beverage, as well as cosmetics and pharmaceutical industries. Practical applications: Recently, there has been a great demand by not only food consumers but also manufacturers for products containing natural ingredients. In this study, a promising nanoemulsion delivery system for oregano as an essential oil model with long‐term stability using a biosurfactant (Quillaja Saponin) was successfully produced by microfluidization. We optimized the formulation in terms of energy requirement and costs, which are important factors from an industry point of view. The fabricated colloidal dispersions have antimicrobial as well as flavoring and potential antioxidant applications in different products including food and beverages, as well as pharmaceutical and beauty products. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
23. Solubilisation of Hydrophobic Drugs by Saponins.
- Author
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VINAROV, Z., RADEVA, DENITSA, KATEV, V., TCHOLAKOVA, SLAVKA, and DENKOV, N.
- Subjects
- *
SAPONINS , *HYDROPHOBIC interactions , *DRUG solubility , *MOLECULAR structure , *QUILLAJA , *HIGH performance liquid chromatography - Abstract
Poor aqueous solubility limits the bioavailability of hydrophobic drugs. Thus, there is an increasing interest in new micelle-forming, drug-solubilizing molecules that can offer improved effectiveness and safety. The effect of 13 saponin extracts on the solubility of fenofibrate and danazol was studied and the relationship between saponin molecular structure and drug solubilisation capacity was assessed. Drug solubility was measured by high-performance liquid chromatography and saponin solubilisation capacity was compared to the conventional surfactant Brij-35. Saponins from Quillaja saponaria and Camellia oleifera improved the aqueous solubility of danazol and fenofibrate by more than two orders of magnitude. For danazol, the solubilisation capacity of the best saponins was 2-3 times higher than Brij-35, whereas for fenofibrate, it was slightly lower than the reference surfactant. Both drugs were solubilized very effectively by bidesmosidic oleanane saponins, whereas dammarane, mono- and tri-desmosidic oleanane saponins and steroid (furostanol and spirostanol) saponins had no effect on drug solubility. Exceptions were fenusterol, a furostanol saponin and escin, a monodesmosidic oleanane saponin, which solubilised danazol only. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
24. Concentration effect of Quillaja saponin – Co-surfactant mixtures on emulsifying properties.
- Author
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Reichert, Corina L., Salminen, Hanna, Badolato Bönisch, Gabriela, Schäfer, Christian, and Weiss, Jochen
- Subjects
- *
QUILLAJA , *SURFACE active agents , *STABILIZING agents , *OIL-water interfaces , *LECITHIN , *FOOD additives - Abstract
Hypothesis This study examined the emulsifying properties of mixed surfactant systems of Quillaja saponins and food-grade co-surfactants (Na-caseinate, pea protein, rapeseed lecithin, and egg lecithin). We hypothesized to these mixtures may build mixed adsorption layers and thus enhance emulsion stabilization. Experiments Oil-in-water emulsions (10%, pH 7) were prepared with different concentrations of co-surfactants (0.1–5.0%) alone or mixed with Quillaja saponins (0.05 or 0.5%). Dynamic interfacial tension measurements were performed to characterize the behavior of the surfactants at an oil-water interface. Findings Low Quillaja saponin concentrations led to either no changes or substantial increases in particle sizes of protein stabilized emulsions, but d 43 -values decreased in lecithin stabilized emulsions at low lecithin concentrations. The dominating effect of Quillaja saponins at high concentrations led to formation of small droplets (d 43 ≤ 2 µm) in all emulsions, except with 2.5% pea proteins. All co-surfactants showed synergistic or additive effects with respect to interfacial tension reductions upon addition of Quillaja saponins (except for egg lecithin with 0.005% Quillaja saponin addition). The results indicated a competing effect for saponin–protein interfaces, but formation of mixed saponin–lecithin interfaces, thus showing that the emulsion stabilization and interfacial properties can be tuned by specific binary surfactant mixtures. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
25. Evaluation of plant-derived preparations and microorganisms as seed treatments for control of covered kernel smut of sorghum (Sporisorium sorghi).
- Author
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Moharam, Moustafa H. A., Stephan, Dietrich, and Koch, Eckhard
- Subjects
- *
BACILLUS amyloliquefaciens , *TRICHODERMA harzianum , *SEED treatment - Published
- 2018
- Full Text
- View/download PDF
26. Quillaja saponaria Saponins with Potential to Enhance the Effectiveness of Disinfection Processes in the Beverage Industry.
- Author
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Antolak, Hubert, Mizerska, Urszula, Berłowska, Joanna, Otlewska, Anna, and Kręgiel, Dorota
- Subjects
QUILLAJA ,DISINFECTION & disinfectants ,BEVERAGE industry - Abstract
This study examines the in vitro effect of Quillaja saponaria extracts on Asaia spp. planktonic cells and biofilms, in comparison and combination with two disinfectants: peracetic acid and N-ethyl-N,N-dimethylhexadecylammonium bromide. The growth of six bacterial strains was evaluated spectrophotometrically. Biofilm eradication was determined using the plate count method and luminometry. The planktonic cells were characterized by relatively high resistance to peracetic acid and higher sensitivity to N-ethylo-N,N-dimethylohexadecylioamonium bromide. In almost all the tested strains, growth was inhibited by 0.125% (v/v) peracetic acid and 0.0313% (w/v) quaternary ammonium compound. However, combinations of cell pretreatment using saponin and peracetic acid action were the most efficient against both planktonic and biofilm cells. The minimum inhibitory concentrations for peracetic acid were 4-8 times lower than those for bacterial strains without preliminary saponin action. Eradication of Asaia spp. biofilms reduced the number of living cells by 4-5 logarithmic units. These results demonstrate the synergetic action of saponin extract and disinfectant, and could be useful in the development of industrial strategies against Asaia spp. biofilms. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
27. Leaf saponins of Quillaja brasiliensis enhance long-term specific immune responses and promote dose-sparing effect in BVDV experimental vaccines.
- Author
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Cibulski, Samuel, Rivera-Patron, Mariana, Suárez, Norma, Pirez, Macarena, Rossi, Silvina, Yendo, Anna Carolina, de Costa, Fernanda, Gosmann, Grace, Fett-Neto, Arthur, Roehe, Paulo Michel, and Silveira, Fernando
- Subjects
- *
BOVINE viral diarrhea , *SAPONINS , *QUILLAJA , *VIRAL vaccines , *IMMUNE response , *DRUG dosage , *PREVENTION , *THERAPEUTICS - Abstract
Saponin-based adjuvants are promising adjuvants that enhance both humoral and T-cell-mediated immunity. One of the most used natural products as vaccine adjuvants are Quillaja saponaria bark saponins and its fraction named Quil A®. Despite that, its use has been restricted for human use due to safety issues. As an alternative, our group has been studying the congener species Quillaja brasiliensis saponins and its performance as vaccine adjuvants, which have shown to trigger humoral and cellular immune responses comparable to Quil A® but with milder side effects. Here, we studied a semi purified aqueous extract (AE) and a previously little characterized saponin-enriched fraction (QB-80) from Q. brasiliensis as vaccine adjuvants and an inactivated virus (bovine viral diarrhea virus, BVDV) antigen co-formulated in experimental vaccines in mice model. For the first time, we show the spectra pattern of the Q. brasiliensis saponins by MALDI-TOF, a novel and cost-effective method that could be used to characterize different batches during saponins production. Both AE and QB-80 exhibited noteworthy chemical similarities to Quil A®. In addition, the haemolytic activity and toxicity were assessed, showing that both AE and QB-80 were less toxic than Quil A®. When subcutaneously inoculated in mice, both fractions promoted long-term strong antibody responses encompassing specific IgG1 and IgG2a, enhanced the avidity of IgG antibodies, induced a robust DTH reaction and significantly increased IFN-ɣ production in T CD4 + and T CD8 + cells. Furthermore, we have proven herein that AE has the potential to promote dose-sparing, substantially reducing the dose of antigen required for the BVDV vaccines and still eliciting a mixed Th1/Th2 strong immune response. Based on these results, and considering that AE is a raw extract, easier and cheaper to produce than commercially available saponins, this product can be considered as candidate to be escalated from experimental to industrial uses. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
28. Quillaja saponaria bark saponin protects Wistar rats against ferrous sulphate-induced oxidative and inflammatory liver damage.
- Author
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Ahmed Abdel-Reheim, Mustafa, Messiha, Basim Anwar Shehata, and Abo-Saif, Ali Ahmed
- Subjects
- *
QUILLAJA , *SAPONINS , *MEDICINAL plants , *HEPATOTOXICOLOGY , *LIVER disease treatment , *LABORATORY rats - Abstract
Context:Saponins from different sources are historically reported in Chinese medicine to possess many beneficial effects. However, insufficient experimental data are available regarding the hepatoprotective potential ofQuillajabark saponin. Objective:The protective effect ofQuillaja saponariaMolina (Quillajaceae) bark triterpenoid saponin against iron-induced hepatotoxicity is compared to the standardN-acetylcysteine in adult male Wistar rats. Materials and methods:Animals were divided into (six) groups, namely a normal control, anN-acetylcysteine control (300 mg/kg/day, p.o., 10 days), a saponin control (100 mg/kg/day, p.o., for 10 days), a hepatotoxicity control (two doses of ferrous sulphate, 30 mg/kg/day each, i.p., on 9th and 10th day), anN-acetylcysteine plus ferrous sulphate (standard treatment) and a saponin plus ferrous sulphate (test treatment) group. Hepatocyte integrity loss markers (serum ALT, AST, ALP, GGT and LDH), oxidative stress markers (hepatic MDA, GSH and NOx), dyslipidaemic markers (serum TC and TG) and hepatocyte functioning markers (serum bilirubin and albumin) were assessed. Results:Quillajabark saponin decreased iron-induced elevation of ALT (reaching 57% of hepatotoxicity control), AST (66%), ALP (76%), GGT (60%), LDH (54%), MDA (65%), NOx(77%), TC (70%), TG (54%), and total (54%), direct (54%) and indirect (54%) bilirubin, coupled with increased GSH (219%) and albumin (159%) levels. Histopathological study strongly supported biochemical estimations, while immunohistochemical study showed marked effect on eNOS and iNOS expression. Conclusions:Quillajabark saponin has a good hepatoprotective effect. Amelioration of oxidative stress and suppression of NOS expression, with resultant maintenance of hepatocyte integrity and functioning, may explain this beneficial effect. [ABSTRACT FROM PUBLISHER]
- Published
- 2017
- Full Text
- View/download PDF
29. Emulsification efficacy of Quillaja saponins at very low concentration: Model development and role of alcohols.
- Author
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Schober, Amanda, Zhang, Jian, Subramaniam, Anand, and Normand, Valery
- Subjects
- *
QUILLAJA , *BIOSURFACTANTS , *EMULSIONS , *ALCOHOLS (Chemical class) , *LIMONENE - Abstract
The present study aims at quantifying interfacial coverage of a biosurfactant ( Quillaja saponins) and understanding the impact of flavor and fragrance alcohols on emulsification efficacy of the biosurfactant in a surfactant-oil-matrix system. Emulsions were prepared using limonene, alkanes (C 8 , C 12 , and C 16 ) or limonene ̶ alcohol (linalool and C 6 C 10 alcohols) mixtures at different ratios as oil phase stabilized by Quillaja saponins at very low concentrations (0.005–0.05% w/w). Droplet size was measured and size distributions were numerized to determine surface and volume average droplet diameters of bimodal emulsions. Using a model developed in the present study, Quillaja saponins showed an interfacial coverage of 5.0 × 10 6 cm 2 /g and a head surface of 1.37 nm 2 with a lay-on configuration at interface. The model proved to discriminate between surface active (alcohols) and non-active (alkanes) compounds. The apparent interfacial coverage of saponins increased linearly with increasing alcohol concentration. The type of alcohol (terpene alcohol vs. medium chain alcohols) and alcohol chain length (C 6 C 10 ) showed little impact on emulsification efficacy of Quillaja saponins. The molar ratio of heptanol to saponin at interface increased from 0 to 8.6 corresponding to 0–30% w/w heptanol in limonene. This study revealed that the distribution of alcohol at interface was mainly driven by partitioning in the surfactant-oil-matrix system. The practical implication of the present study is to enhance emulsification efficacy of Quillaja Saponins at very low concentration by incorporating surface active compounds, i.e. flavor or fragrance alcohols. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
30. Effect of dried and extrudate of bitter gourd fruit on epithelial microflora in raw chicken legs meat.
- Author
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Abdelkarim, H., Rukayadi, Y., Abdulkarim, S. M., and Sulaiman, R.
- Subjects
SAPONINS ,QUILLAJA ,FICUS (Plants) ,LACTOBACILLUS ,PHENOLIC resins - Abstract
Plants have been used recently to eliminate bacterial growth in food products. This study was undertaken to test the in vitro sanitizing effect of crude extract from bitter gourd (BG) fruit on the growth of native microorganisms in raw chicken leg meat. Hot air dried BG and extrudate extracts at 1% concentration and exposure times of (5, 10 and 15 min) were used to treat the samples using dilution method. Results showed that BG extrudate had a slightly stronger bactericidal activity against the microflora than the B.G. hot air drying treatment, especially, on E. coli at all exposure time. Overall, there is no significant difference between the treatments; Total Plate Count (TPC), Escherichia coli, Bacillus cereus, Staphylococcus aureus. The best reduction time of microflora by hot air dried extract was at (15 min) except for B. cereus was at (5 min) and for extrudate extract was at (5 min) except for E. coli was at (10 min). In conclusion, bitter gourd extract could be used as an important natural sanitizer for rinsing raw food matrials such chicken meat. [ABSTRACT FROM AUTHOR]
- Published
- 2017
31. A laboratory study of a novel bio-based nonionic surfactant to mitigate clay swelling
- Author
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Saeed Khezerloo-ye Aghdam, Mohammad Hossein Ahmadi, and Alireza Kazemi
- Subjects
Thermogravimetric analysis ,020209 energy ,Clay swelling ,Quillaja saponin ,Energy Engineering and Power Technology ,02 engineering and technology ,Contact angle ,020401 chemical engineering ,Pulmonary surfactant ,Bio-based surfactant ,Geochemistry and Petrology ,0202 electrical engineering, electronic engineering, information engineering ,Zeta potential ,medicine ,TA703-712 ,Petroleum refining. Petroleum products ,0204 chemical engineering ,biology ,Chemistry ,Geology ,Engineering geology. Rock mechanics. Soil mechanics. Underground construction ,Wettability alteration ,Geotechnical Engineering and Engineering Geology ,biology.organism_classification ,Surface coating ,Fuel Technology ,Chemical engineering ,Quillaja ,Particle size ,Swelling ,medicine.symptom ,TP690-692.5 - Abstract
This study reports the applicability of quillaja saponin (QS) as a vigorous and environmentally friendly shale swelling inhibitor. QS is a natural surfactant, which is extracted from herbal sources. The inhibition strength of this surfactant was assessed through various experiments, such as sedimentation, inhibition, filtration, particle size, Scanning Electron Microscope (SEM) images, and cutting recovery. Data obtained from these tests illustrated that QS greatly inhibits clays from swelling. The optimal concentration for QS in this intend was 10 g/L. Compatibility of this surfactant with other common additives was also investigated, which showed that it is totally compatible. Finally, the potential inhibition mechanism was assessed through thermal gravimetric analysis (TGA), zeta potential, and contact angle measurement experiments. Surface coating, and wettability alteration of clay particles to the oil-wet state was recognized as the most probable mechanism.
- Published
- 2021
32. A Bioinformatics Tool for Efficient Retrieval of High-Confidence Terpene Synthases (TPS) and Application to the Identification of TPS in Coffea and Quillaja
- Author
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Douglas S, Domingues, Liliane S, Oliveira, Samara M C, Lemos, Gian C C, Barros, and Suzana T, Ivamoto-Suzuki
- Subjects
Alkyl and Aryl Transferases ,Terpenes ,Quillaja ,Computational Biology ,Humans ,Coffea ,Phylogeny - Abstract
Terpenoids are a class of compounds that are found in all living organisms. In plants, some terpenoids are part of primary metabolism, but most terpenes found in plants are classified as specialized metabolites, encoded by terpene synthases (TPS). It is not obvious how to assign the putative product of a given TPS using bioinformatics tools. Phylogenetic analyses easily assign TPS into families; however members of the same TPS family can synthetize more than one terpenoid-and, in many biotechnological applications, researchers are more interested in the product of a given TPS rather than its phylogenetic profile. Automated protein annotation can be used to classify TPS based on their products, despite the family they belong to. Here, we implement an automated bioinformatics method, search_TPS, to identify TPS proteins that synthesize mono, sesqui and diterpenes in Angiosperms. We verified the applicability of the method by classifying wet lab validated TPS and applying it to find TPS proteins in Coffea arabica, C. canephora, C. eugenioides, and Quillaja saponaria. Search_TPS is a computational tool based on PERL scripts that carries out a series of HMMER searches against a curated database of TPS profile hidden Markov models. The tool is freely available at https://github.com/liliane-sntn/TPS .
- Published
- 2022
33. Soapbark Triterpenes: Quillaja brasiliensis Cell Culture Sapogenin and Free Sterol Analysis by GCMS
- Author
-
Yve V S, Magedans and Michael A, Phillips
- Subjects
Sterols ,Sapogenins ,Quillaja ,Cell Culture Techniques ,Phytosterols ,Saponins ,Gas Chromatography-Mass Spectrometry ,Triterpenes - Abstract
Triterpene saponins of the genus Quillaja (Quillajaceae) are known for their immunoadjuvant, hypocholesterolemic, and anti-inflammatory activity. Plant cell cultures are useful for the study of saponin metabolism and industrial production of these bioactive compounds. While structurally related phytosterols are primary metabolites essential to growth and development, saponins are responsive to pathogen and abiotic stress, fulfilling roles in plant specialized metabolism. For cell culture production of saponins, phytosterols may be considered a competing pathway which relies on a common pool of cytosolic isoprenoid precursors.Understanding the metabolic allocation of resources between these two related pathways is key to maximizing saponin production in in vitro production systems. Sterols and saponins naturally occur in multiple conjugated forms, which complicate separation and quantification. The acid hydrolysis of conjugated sterols and saponins to their free forms is a useful technique to simplify their analysis by gas chromatography. Here we provide the workflow for the quantification of free sterols and sapogenins in cell cultures of Quillaja brasiliensis .
- Published
- 2022
34. Formulation of IMXQB: Nanoparticles Based on Quillaja brasiliensis Saponins to be Used as Vaccine Adjuvants
- Author
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Mariana, Rivera-Patron, Samuel P, Cibulski, Iris, Miraballes, and Fernando, Silveira
- Subjects
Mammals ,Adjuvants, Immunologic ,Malaria Vaccines ,Quillaja ,Animals ,Humans ,Nanoparticles ,Saponins ,Adjuvants, Vaccine ,Quillaja Saponins ,ISCOMs - Abstract
Adjuvants are essential components of subunit, recombinant, nonreplicating and killed vaccines, as they are substances that boost, shape, and/or enhance the immune response triggered by vaccination. Saponins obtained from the Chilean Q. saponaria tree are used as vaccine adjuvants in commercial vaccines, although they are scarce and difficult to obtain. In addition, tree felling is needed during its extraction, which has ecological impact. Q. brasiliensis leaf-extracted saponins arise as a more sustainable alternative, although its use is still limited to preclinical studies. Despite the remarkable immunostimulating properties of saponins, they are toxic to mammalian cells, due to their intrinsic characteristics. For these reasons they are mostly used in veterinary vaccines, although recently the Q. saponaria purified saponin QS-21 has been included in adjuvant systems for human vaccines, such as Mosquirix and Shingrix (GSK). In order to abrogate the toxicity of the saponins fractions, they can be formulated as immunostimulating complexes (ISCOMs). ISCOM-matrices are cage-like nanoparticles of approximately 40 nm, formulated combining saponins and lipids, without antigen, and are great adjuvants able to promote Th1-biased immune responses in a safe manner. Herein we describe how to formulate ISCOM-matrices nanoparticles using Q. brasiliensis purified saponin fractions (IMXQB) by the dialysis method. In addition, we indicate how to verify the appropriate size and homogeneity of the formulated nanoparticles.
- Published
- 2022
35. In Vitro Gastrointestinal Stability of Lipophilic Polyphenols is Dependent on their Oil–Water Partitioning in Emulsions: Studies on Curcumin, Resveratrol, and Quercetin
- Author
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Bingjing Zheng, David Julian McClements, and Hualu Zhou
- Subjects
0106 biological sciences ,biology ,010401 analytical chemistry ,food and beverages ,General Chemistry ,Resveratrol ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,Bioavailability ,chemistry.chemical_compound ,chemistry ,Polyphenol ,Quillaja ,Lipid droplet ,Curcumin ,Food science ,Solubility ,General Agricultural and Biological Sciences ,Quercetin ,010606 plant biology & botany - Abstract
Many lipophilic polyphenols have low bioavailability because of their poor solubility and chemical stability within the human gut. The encapsulation of these polyphenols within digestible lipid droplets can improve their solubility and stability. However, there is currently a poor understanding of how the molecular and physicochemical properties of specific polyphenols impact these characteristics. In this study, the factors influencing the solubility and stability of different polyphenols (curcumin, resveratrol, and quercetin) under simulated gastrointestinal conditions were examined when they were delivered in the form of soybean oil-in-water nanoemulsions containing quillaja saponin-coated droplets (d32 ≈ 0.15 μm; ζ = -63 mV; pH 5). The polyphenols were loaded into the lipid droplets using a pH-driven method, which is based on the pH-dependent electrical charge, oil-water partitioning, and water-solubility of these molecules. The encapsulation efficiency of all three polyphenols was relatively high (75-87%). However, their chemical stability under gastrointestinal conditions (i.e., the % remaining after exposure to gastrointestinal conditions) differed considerably: quercetin (44%), curcumin (92%), and resveratrol (100%). This effect was mainly attributed to the lower logD value of quercetin (2.17) than those of resveratrol (3.39) and curcumin (4.12). As a result, a high fraction (>50%) of quercetin was located within the aqueous gastrointestinal fluids, where it would be more prone to chemical degradation or precipitation. The fraction of the polyphenols solubilized in the gastrointestinal fluids (bioaccessibility) followed a different trend: curcumin (57%) < quercetin (73%) < resveratrol (76%). This effect was attributed to the chemical instability and/or binding of curcumin with other molecules in the simulated intestinal conditions. These results provide useful information for designing nanoemulsion-based delivery systems to improve the efficacy of lipophilic polyphenols.
- Published
- 2021
36. Bioaccessibility of oil-soluble vitamins (A, D, E) in plant-based emulsions: impact of oil droplet size
- Author
-
Hualu Zhou, Zhiyun Zhang, David Julian McClements, and Yunbing Tan
- Subjects
0301 basic medicine ,Vitamin ,Retinyl Esters ,medicine.medical_treatment ,Biological Availability ,Capsules ,In Vitro Techniques ,03 medical and health sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,medicine ,Vitamin D and neurology ,Vitamin E ,Food science ,Particle Size ,Vitamin D ,Micelles ,Vitamin E Acetate ,Drug Carriers ,030109 nutrition & dietetics ,biology ,04 agricultural and veterinary sciences ,General Medicine ,Lipid Metabolism ,biology.organism_classification ,040401 food science ,Soybean Oil ,Gastrointestinal Tract ,Drug Liberation ,Solubility ,chemistry ,Quillaja ,Oil droplet ,Emulsion ,Digestion ,Emulsions ,Diterpenes ,Hydrophobic and Hydrophilic Interactions ,Lipid digestion ,Food Science - Abstract
We systematically investigated the impact of oil droplet diameter (≈0.15, 1.6, and 11 μm) on the bioaccessibility of three oil-soluble vitamins (vitamin A palmitate, vitamin D, and vitamin E acetate) encapsulated within soybean oil-in-water emulsions stabilized by quillaja saponin. Lipid digestion kinetics decreased with increasing droplet size due to the reduction in oil-water interfacial area. Vitamin bioaccessibility decreased with increasing droplet size from 0.15 to 11 μm: 87 to 39% for vitamin A; 76 to 44% for vitamin D; 77 to 21% for vitamin E. Vitamin bioaccessibility also decreased as their hydrophobicity and molecular weight increased, probably because their tendency to remain inside the oil droplets and/or be poorly solubilized by the mixed micelles increased. Hydrolysis of the esterified vitamins also occurred under gastrointestinal conditions: vitamin A palmitate (∼90%) and vitamin E acetate (∼3%). Consequently, the composition and structure of emulsion-based delivery systems should be carefully designed when creating vitamin-fortified functional food products.
- Published
- 2021
37. Occupational immediate type allergy to soapnut and quillaja bark
- Author
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Ingrid Sander, Rolf Merget, and Monika Raulf
- Subjects
Allergy ,Low protein ,Saponin ,Case Report ,soapnut ,complex mixtures ,quillaja bark ,Medicine ,occupational ,saponin ,General Environmental Science ,chemistry.chemical_classification ,biology ,Inhalation ,Traditional medicine ,business.industry ,General Engineering ,asthma ,biology.organism_classification ,medicine.disease ,chemistry ,Bronchial hyperresponsiveness ,Quillaja ,visual_art ,Exhaled nitric oxide ,visual_art.visual_art_medium ,General Earth and Planetary Sciences ,Bark ,business - Abstract
A 58-year-old non-atopic chemical worker complained about work-related asthma and rhinoconjunctivitis about 4 years after exposure to quillaja bark and soapnut. Bronchial hyperresponsiveness was demonstrated after withdrawal of medication for 12 hours. Skin prick tests with extracts from quillaja bark and soapnut from the workplace were positive, but ImmunoCAP was positive only with quillaja bark, probably due to the low protein content of the extract from soapnut. Sensitizations to quillaja bark and soapnut, but not to saponin were demonstrated by immunoblot. An inhalation test with a dosimeter was positive with the soapnut extract. A link between disease and exposure was documented by serial measurements of exhaled nitric oxide at and off work, despite preventive measures. A diagnosis of occupational allergy due to quillaja bark and soapnut was made. Further exposure reduction was recommended.
- Published
- 2021
38. IMXQB-80: A Quillaja brasiliensis saponin-based nanoadjuvant enhances Zika virus specific immune responses in mice
- Author
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Matheus Fabião de Lima, Paulo Michel Roehe, Fernando Silveira, Samuel Paulo Cibulski, Diogo O. Souza, Marcelo Sobral da Silva, Yuri Mangueira do Nascimento, Patrícia Sesterheim, Josean Fechine Tavares, Gabriela Casanova, Ana Paula Muterle Varela, and Thais Fumaco Teixeira
- Subjects
medicine.medical_treatment ,030231 tropical medicine ,Saponin ,Immunoadjuvant ,Quillaja Saponins ,Microbiology ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Adjuvants, Immunologic ,Antigen ,medicine ,Saponaria ,Animals ,030212 general & internal medicine ,chemistry.chemical_classification ,General Veterinary ,General Immunology and Microbiology ,biology ,Zika Virus Infection ,Quillaja ,Immunity ,Public Health, Environmental and Occupational Health ,Zika Virus ,Saponins ,biology.organism_classification ,Infectious Diseases ,chemistry ,biology.protein ,Molecular Medicine ,Antibody ,Adjuvant - Abstract
Vaccine adjuvants are compounds that enhance/prolong the immune response to a co-administered antigen. Saponins have been widely used as adjuvants for many years in several vaccines – especially for intracellular pathogens – including the recent and somewhat revolutionary malaria and shingles vaccines. In view of the immunoadjuvant potential of Q. brasiliensis saponins, the present study aimed to characterize the QB-80 saponin-rich fraction and a nanoadjuvant prepared with QB-80 and lipids (IMXQB-80). In addition, the performance of such adjuvants was examined in experimental inactivated vaccines against Zika virus (ZIKV). Analysis of QB-80 by DI-ESI-ToF by negative ion electrospray revealed over 29 saponins that could be assigned to known structures existing in their congener Q. saponaria, including the well-studied QS-21 and QS-7. The QB-80 saponins were a micrOTOF able to self-assembly with lipids in ISCOM-like nanoparticles with diameters of approximately 43 nm, here named IMXQB-80. Toxicity assays revealed that QB-80 saponins did present some haemolytical and cytotoxic potentials; however, these were abrogated in IMXQB-80 nanoparticles. Regarding the adjuvant activity, QB-80 and IMXQB-80 significantly enhanced serum levels of anti-Zika virus IgG and subtypes (IgG1, IgG2b, IgG2c) as well as neutralized antibodies when compared to an unadjuvanted vaccine. Furthermore, the nanoadjuvant IMXQB-80 was as effective as QB-80 in stimulating immune responses, yet requiring fourfold less saponins to induce the equivalent stimuli, and with less toxicity. These findings reveal that the saponin fraction QB-80, and particularly the IMXQB-80 nanoadjuvant, are safe and capable of potentializing immune responses when used as adjuvants in experimental ZIKV vaccines.
- Published
- 2021
39. Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Biji Kelor (Moringa oleifera Lam.) dengan Metode Spektrofotometri UV-Vis
- Author
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Joni Tandi, Tien Wahyu Handayani, and Yulistien Yusuf
- Subjects
chemistry.chemical_classification ,Traditional medicine ,biology ,Saponin ,Orange (colour) ,biology.organism_classification ,Rotary evaporator ,Moringa ,chemistry.chemical_compound ,chemistry ,Quillaja ,Tannic acid ,Maceration (wine) ,Tannin - Abstract
This study aims to determine the content and total levels of secondary metabolites of ethanol extract of moringa seed (Moringa oleifera Lam.). Extraction of moringa seed using the maceration method to obtain a filtrate. The filtrate obtained was concentrated using a rotary evaporator to obtain a concentrated extract. The extract was tested qualitatively for alkaloids, flavonoids, saponins, and tannins using a suitable reagent with the test parameters. The quantitative test was using UV-Visible spectrophotometry. Alkaloids using test parameters equivalent total alkaloid quinine, flavonoids use parameter test equivalent of the total flavonoids quercetin, saponin using test parameters from Quillaja total saponins and tannins quantitative bark using test parameters total tannin tannic acid equivalent. The result showed that moringa seed positive for alkaloids characterized by orange deposition, flavonoids are characterized by the formation of the yellow color orange, saponin their stable foam, and tannins are marked in black. Quantitative test results alkaloids of 916,87 µg/g, flavonoids of 0.255%, saponin of 6.367%, and tannin of 3,724.5 µg/g. Keywords: Moringa oleifera Lam., secondary metabolites, spectrophotometry
- Published
- 2020
40. Oil–Water Interfacial-Directed Spontaneous Self-Assembly of Natural Quillaja Saponin for Controlling Interface Permeability in Colloidal Emulsions
- Author
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Xiao-Wei Chen, Chuan-Guo Ma, Xiao-Quan Yang, and Shangde Sun
- Subjects
Materials science ,biology ,Intermolecular force ,Stacking ,General Chemistry ,biology.organism_classification ,Molecular dynamics ,Colloid ,Chemical engineering ,Quillaja ,Amphiphile ,Emulsion ,Self-assembly ,General Agricultural and Biological Sciences - Abstract
Assembly of amphiphiles at the interface of two immiscible fluids is of great scientific and technological interest in offering efficient routes to smart vehicles for functional deliveries. Natural Quillaja saponin (QS) has gathered widespread interest within the scientific community as a result of its unique interfacial properties. Herein, spontaneously interface-driven self-assembly (SIDSA) of QS at the oil-water interface was systematically studied by morphology and spectroscopy. It was found to self-assemble into a micrometer-scale network in helical fibers by combined intermolecular π-π stacking and hydrogen bonding among saponins at the liquid-liquid interface. From SIDSA, multilayer films on the surfaces of dispersed droplets were formed and enhanced emulsion stability. Interfacial QS-based films on droplet surfaces were also shown to confine interfacial diffusion processes by serving as transport barriers. Furthermore, they can be exploited to control the release of volatiles from the dispersed liquid phase by regulating the interface film, which is shown by molecular dynamics to occur through a hydrogen-bonded mechanism. These results provide new insight into the interfacial assembly structure that can enable unique controllable release in a broad range of applications in food, beverages, pharmaceuticals, and cosmetics.
- Published
- 2020
41. Structure Elucidation of Triterpenoid Saponins Found in an Immunoadjuvant Preparation of
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Federico, Wallace, Carolina, Fontana, Fernando, Ferreira, and Cristina, Olivaro
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Magnetic Resonance Spectroscopy ,Adjuvants, Immunologic ,Quillaja ,Humans ,Saponins ,Mass Spectrometry ,Triterpenes - Abstract
An immunoadjuvant preparation (named Fraction B) was obtained from the aqueous extract of
- Published
- 2022
42. Effects of N-acylation on the immune adjuvanticity of analogs of the Quillaja saponins derivative GPI-0100
- Author
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Dante J. Marciani
- Subjects
Mice ,Infectious Diseases ,General Veterinary ,General Immunology and Microbiology ,Adjuvants, Immunologic ,Acylation ,Immunoglobulin G ,Quillaja ,Public Health, Environmental and Occupational Health ,Molecular Medicine ,Animals ,Saponins ,Quillaja Saponins - Abstract
Modification of the 3-glucuronic acid (GlcA) residue from the Quillaja saponin (QS) adjuvants by N-acylation, yields derivatives with linear alkylamides that show structural and functional changes. Structural, since the relatively unreactive added hydrophobic alkyl chains may modify these glycosides' conformation and micellar structure. Functional, because altering the availability of proposed pharmacophores, like fucose (Fucp) and aldehyde groups, to interact with their cellular receptors, may change these glycosides' adjuvanticity. While deacylated QS (DS-QS) adjuvants bias the response toward a sole anti-inflammatory Th2 immunity against an antigen, their N-alkylated derivatives carrying octyl to dodecylamide residues, modify that response to a pro-inflammatory Th1 immunity. As shown by their IgG2a/IgG1 titer ratios, which are higher than those for Th2 immunity. A result of the fact that in mice, the IgG2a levels are dependent on the direct influence of secreted interferon-γ (IFN-γ), a crucial Th1 cytokine. But addition of the longer and more lipophilic tetradecylamide group, yields derivatives that like DS-QS induce Th2 immunity, as shown by their low IgG2a/IgG1 ratio. Results that imply that changes in these analogs' conformation and micellar structure, would affect the immunomodulatory properties or adjuvanticity of N-acylated DS-QS. Physical changes that may alter the availability of groups like Fucp, to bind to its presumed dendritic cells' lectin receptor DC-SIGN; an essential step in the stimulation of Th2 immunity. Structural properties that in an aqueous environment, would depend on these glycosides' balance of their hydrophilic and lipophilic moieties (HLB), and the interactions of the newly introduced alkyl chain with the native QS' lipophilic triterpene aglycone and hydrophilic oligosaccharide chains. A situation that would explain these new derivatives' qualitative and quantitative changes in adjuvanticity.
- Published
- 2022
43. Produção de saponinas triterpênicas bioativas em cultivos celulares de Quillaja brasiliensis
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Magedans, Yve Verônica da Silva and Fett Neto, Arthur Germano
- Subjects
Vaccines ,Quillaja ,Saponinas ,Adjuvants ,Terpenos ,Cultura de celulas - Abstract
Quillaja brasiliensis é uma espécie arbórea conhecida popularmente como pau-sabão, que produz saponinas triterpênicas em suas folhas. As saponinas de Q. brasiliensis apresentam pronunciada atividade adjuvante em vacinas veterinárias experimentais, de forma comparável ao produto comercial Quil-A®, obtido de cascas de Quillaja saponaria. Adjuvantes são adicionados às vacinas inativadas para aumentar a potência e duração da resposta imune, garantindo a segurança e eficiência dessas formulações. Tendo em vista a possibilidade da utilização de Q. brasiliensis para a obtenção de saponinas com potencial uso comercial e considerando a necessidade de maior conhecimento sobre a produção e acúmulo sustentável de metabólitos ativos em geral, esta tese visou definir procedimentos para estabelecer culturas celulares de Q. brasiliensis e avaliar sua competência e viabilidade para produção de saponinas. Extensa análise da literatura de metabolismo especializado de plantas resultou em dois artigos de revisão, um voltado a saponinas imunoadjuvantes de Quillaja sp. e outro, à biotecnologia e biossíntese de saponinas triterpênicas. Cultivos celulares foram estabelecidos, sendo que o ciclo de cultivo de suspensões celulares foi definido como sendo de 21 dias. A produção de saponinas acompanhou o acúmulo da massa seca celular, e conteúdo máximo de saponinas coincide com o final da fase exponencial de crescimento. A caracterização química inicial das saponinas produzidas por suspensões celulares foi obtida. Sete compostos foram tentativamente identificados por LC-MS, e os padrões de fragmentação indicam que ácido quiláico foi a principal aglicona presente. As saponinas purificadas de suspensões celulares foram testadas em um ensaio com vacinas adjuvantadas contra o vírus da gripe. Houve um aumento de anticorpos em indivíduos vacinados com vacinas adjuvantadas com extratos celulares, principalmente IgG total e IgG2, contudo em um nível menor que as formulações adjuvantadas com compostos purificados de folhas de Q. brasiliensis. Além disso, protocolos de quantificação de saponinas e esteróis por GC-MS foram desenvolvidos. Estes protocolos irão subsidiar estudos de fluxo metabólico e avaliação do investimento de carbono nos pools desses compostos em suspensões celulares. Em suma, cultivos celulares de Q. brasiliensis constituem uma fonte de saponinas bioativas e representam uma excelente plataforma para o estudo da biossíntese destes produtos naturais. Quillaja brasiliensis is a tree species popularly known as soap-tree, which produces triterpenoid saponins in its leaves. Saponins purified from leaves of Q. brasiliensis show pronounced adjuvant activity in experimental veterinary vaccines, comparable to the commercial product Quil-A®. Quil-A is purified from barks and woods of Quillaja saponaria. Adjuvants are added to inactivated vaccines to increase potency and extent of the immune response, ensuring safety, and increasing formulation efficacy. Considering the possible use of Q. brasiliensis for production of bioactive saponins and considering the need for advancing the knowledge about the sustainable supply of active metabolites in general, this thesis aimed at defining procedures to establish cell cultures of Q. brasiliensis as a potential source of saponins. Extensive analyses of the literature on plant specialized metabolism resulted in two review articles, one examining immunoadjuvant saponins of Quillaja sp. and the other focusing on plant triterpene saponin biotechnology and biosynthesis. Q. brasiliensis cell cultures were successfully established. Cell suspension culture cycle lasted 21 days, and the production of saponins followed the accumulation of cell dry mass. Chemical characterization of saponins produced by cell suspensions showed seven compounds tentatively identified by LC-MS. Fragmentation patterns indicated quillaic acid as the main aglycone present in cell extracts. Saponins purified from cell suspensions were tested in an assay for adjuvant activity, using formulations against the influenza virus. There was an increase in antibodies in groups that received vaccines prepared with cell extracts, mainly total IgG and IgG2, even though to a lesser extent than those seen for formulations that received saponins fractions purified from leaves of Q. brasiliensis. In addition, protocols for quantification of saponins and sterols by GC-MS were developed. These methods will allow studies of metabolic flux analyses and the evaluation of carbon investment in the pools of these compounds in cell suspensions. To sum up, Q. brasiliensis cell cultures constitute a source of bioactive saponins and can provide a platform for the study of the biosynthesis of these natural products.
- Published
- 2022
44. Stabilization and functionalization of aqueous foams by Quillaja saponin-coated nanodroplets.
- Author
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Chen, Xiao-Wei, Yang, Dan-Xia, Zou, Yuan, and Yang, Xiao-Quan
- Subjects
- *
QUILLAJA , *DROPLETS , *SAPONINS , *GAS-liquid interfaces , *FOAM - Abstract
We report evidence for stabilization and functionalization of aqueous foams stabilized by Quillaja saponin (QS)-coated nanodroplets. In contrast to foams stabilized by QS, stabilized the foams of QS-coated nanodroplets showed superior foamability, stability and multi-functional characteristics. Specifically, the half-life time of the foam stabilized by nanodroplets was approximately 4 times that of saponin. The microstructure observation indicates the nanodroplets from assembly of saponin around oil droplet were strong attachment at the gas-liquid interface and stabling a large gas-liquid interfacial area in a hierarchical structure. The surface dynamic adsorption and large deformation rheology were performed, revealed that QS nanodroplets were almost irreversibly adsorbed at air-liquid interface and exhibited less surface desorption and high elastic-viscous response to a large mechanical deformation. These nanodroplets stabilized foams presented a large capacity for loading hydrophobic flavors and nutrients (e.g., β -carotene and curcumin), which could be used to create a new class of foam food products with sustained release of flavors and/or health benefit functionality. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
45. Influence of homogenization on physical properties of model coffee creamers stabilized by quillaja saponin.
- Author
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Chung, Cheryl, Sher, Alexander, Rousset, Philippe, and McClements, David Julian
- Subjects
- *
STABILIZING agents , *FOOD emulsifiers , *QUILLAJA , *COFFEE processing , *CREAMING (Chemistry) - Abstract
There is a growing demand for use of natural ingredients in food manufacturing. This study utilized a natural emulsifier, quillaja saponin (1%) to fabricate non-dairy model creamer emulsions (containing 10% medium chain triglycerides oil). Varying homogenization conditions, ranging from a high-shear mixer to passing through a microfluidizer at 20,000 psi, were applied to fabricate emulsions. The effect of particle size on the appearance, tristimulus color coordinates, and electrical characteristics of the model creamers and white coffee drinks were investigated. The average droplet size varied from 0.2 to 16 μm. All model creamers had whitish milk-like appearance and the white coffee solutions had light brown color. All systems were physically stable except for the systems with largest oil droplets (1.8 and 16 μm), which had creaming. The lightness, L* (whiteness) of the model creamer and the white coffee increased with decreasing oil droplet size, as smaller droplets scatter more light. Decreasing the oil droplet size led to lower zeta potential (from − 73 to − 54 mV) due to lesser negative charge group accumulated on the interfacial layer of the droplets. The oil droplets were also found to be stable to aggregation in hot acidic coffee solutions prepared using model hard water. Overall, this study found that oil droplets stabilized with natural plant-based surfactant have potential for application in liquid coffee creamers and their stability and whitening power were dependent on the droplet size. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
46. Stability of Emulsions Using a New Natural Emulsifier: Sugar Beet Extract (Beta vulgaris L.).
- Author
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Ralla, Theo, Salminen, Hanna, Edelmann, Matthias, Dawid, Corinna, Hofmann, Thomas, and Weiss, Jochen
- Abstract
This study describes the influence of environmental stresses on the stability of emulsions prepared by a natural sugar beet extract ( Beta vulgaris L.). The emulsion stabilizing performance was compared to that of Quillaja extract, which is widely used within the food and beverage industry as natural surfactant. We investigated the influence of pH, ionic strength, heating and freeze-thawing on the mean particle size, ζ-potential and microstructure of oil-in-water emulsions (10% w/w oil, 0.75% w/w emulsifier). The emulsions stabilized by the anionic sugar beet extract were stable at pH 5-8 and against thermal treatments up to 60 °C. However, the prepared emulsions were unstable at acidic (pH 2-4) and basic pH conditions (pH 9), at high temperature (>60 °C), and at salt additions (> 0.1 M NaCl / CaCl). Moreover, they also phase separated upon freeze-thawing. Our results show that sugar beet extract is capable of stabilizing emulsions and may therefore be suitable as natural emulsifier for selected applications in the food and beverage industry. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
47. Sorption of Quillaja saponaria Molina saponin with chitosan under equilibrium conditions.
- Author
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Smuseva, S., Mironenko, N., Brezhneva, T., Selemenev, V., and Grechkina, M.
- Subjects
- *
QUILLAJA , *MEDICINAL plants , *SAPONINS , *CHITOSAN , *CHITIN - Abstract
Equilibrium sorption of saponin from Quillaja saponaria Molina with chitosan has been analyzed. The shape of the sorption isotherm is determined by the competing processes of the glycoside association in the solution and its absorption with chitosan, as evidenced by the calculated absorption and association energies and the curves of chitosan dehydration accompanying the saponin sorption. Analysis of the chitosan surface during the saponin sorption by means of atomic force microscopy has revealed the structure-morphology features depending on the glycoside concentration in the external solution. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
48. Emulsifying properties of β-lactoglobulin and Quillaja bark saponin mixtures: Effects of number of homogenization passes, pH, and NaCl concentration.
- Author
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de Faria, Janaína Teles, de Oliveira, Eduardo Basílio, Minim, Valéria Paula Rodrigues, and Minim, Luis Antonio
- Subjects
- *
FOOD emulsifiers , *LACTOGLOBULINS , *QUILLAJA , *SAPONINS , *ASYMPTOTIC homogenization , *OIL pollution of water - Abstract
The effects of number of homogenization passes, pH, and NaCl concentration on the formation and stability of oil-in-water emulsions comprising a mixture of a biosurfactant (Quillajabark saponin) and a globular protein (β-lactoglobulin) were investigated. The emulsions were characterized as to visual appearance, droplet size, droplet surface charge, and rheology. The emulsions obtained by different conditions (4, 6, or 8 passes; pH 7, 8, or 9; and 0, 100, or 200 mmol L−1of NaCl) were polydisperse, presented relatively small average droplet sizes (z-average < 323 nm) as well as negative droplet charge (between –20 and –79.6 mV) in all evaluated conditions. Regardless of the number of homogenization passes, the emulsions exhibited low apparent viscosity and pseudoplastic behavior with small yield stress. Viscoelastic behavior was also observed, thus the emulsions were characterized as weak gels. Four homogenization passes were enough to obtain small droplets in the evaluated conditions. Droplet size was not significantly affected by NaCl concentration and pH (p> 0.05). On the other hand, the absolute ζ-potential values significantly decreased and increased upon increased NaCl content and pH, respectively. Regardless of the tested conditions, all emulsions had good stability against phase separation and droplet aggregation, since no significant changes in average droplet size were observed throughout storage (p> 0.05). In the presence of NaCl, in which droplet charge significantly decreased, emulsion was also stable. Thus, we can conclude that electrostatic repulsion as well as steric repulsion was responsible for stabilization. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
49. Performance of Quillaja bark saponin and β-lactoglobulin mixtures on emulsion formation and stability.
- Author
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de Faria, Janaína Teles, de Oliveira, Eduardo Basílio, Minim, Valéria Paula Rodrigues, and Minim, Luis Antonio
- Subjects
- *
QUILLAJA , *SAPONINS , *LACTOGLOBULINS , *EMULSIONS , *HYDROGEN-ion concentration - Abstract
The emulsifying properties of a mixture of Quillaja bark saponin (QBS) and β-lactoglobulin (β-lg) were evaluated under different pH conditions (7–9) and NaCl concentrations (0–200 mmol·L −1 ) and compared to the individual components. The formation and stabilization of oil-in-water emulsions were evaluated through visual analysis, droplet size distribution, droplet surface electrical charge (ζ-potential), and emulsion rheology. Both pH and NaCl concentration affected the properties of these emulsions in different ways, depending upon the QBS:β-lg ratio. QBS and/or β-lg emulsions had a relatively high negative droplet charge at pH ranging from 7 to 9 (−76.7 to −17.8 mV), which decreased in magnitude with decreasing pH or with increasing NaCl concentration. All emulsions were polydisperse and presented relatively small average droplet diameters (236–491 nm). Steady-state flow measurements revealed the non-Newtonian, shear-thinning behavior of all emulsions, which was properly described by the Herschel-Bulkley model with a small yield stress (12.9–214.8 mPa) and low apparent viscosity at 100 s −1 (1.81–2.97 mPa s). The emulsions were characterized as weak gels by dynamic oscillatory measurements. Most of the emulsions comprising QBS, β-lg, or a mixture of both were stable against droplet coalescence over a pH and NaCl concentration range. Regardless of the tested emulsifier, phase separation did not take place, although droplet creaming was observed. Emulsions comprising QBS and β-lg, both independently and mixed, showed similar emulsifying properties. However, the mixture of these appeared to provide emulsions with improved stability when compared to QBS and β-lg emulsions. The good stability of these emulsions can be attributed to the increased electrostatic repulsion and steric stabilization conferred by the two emulsifiers concurrently. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
50. Interactive effects of water stress, container size and fertilizer on survival, gas exchange and morphological traits of Quillaja saponaria seedlings.
- Author
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Espinoza, Sergio E., Santelices, Rómulo E., Cabrera, Antonio M., and Magni, Carlos R.
- Subjects
- *
QUILLAJA , *EFFECT of stress on plants , *GAS exchange in plants , *SEEDLINGS , *PLANT morphology , *FERTILIZERS - Abstract
Quillaja saponaria is a valuable commercial and ecological Chilean native species that has suffered considerably degradation countrywide; however, the determination of the optimum characteristics of the planting stock, an important aspect in restoration projects, has receive little attention. The aim of this study was to evaluate the effect of container size and dose of fertilizer on morphological and physiological responses of Q. saponaria seedlings cultivated in a nursery under well-watered and water restricted conditions. After 27 days growing under contrasting watering regimes, growth, biomass allocation, and gas exchange were measured in 6-month-old seedlings. We found that survival, height and diameter increment, and root dry weight were higher in the water stressed seedlings cultivated in large containers. The water stressed seedlings cultivated in large containers also had superior stomatal conductance, however when the fertilizer dose was decreased, this trait decreased considerably. Thus, a superior performance in a water-restricted environment could be expected in those seedlings cultivated in larger containers with low fertilizer. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
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