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Two experiments were conducted to determine the potential for the essential oil blend Agolin Ruminant L (Agolin) to reduce enteric methane (CH4) emissions from beef cattle when delivered via the drinking water. Experiment 1 evaluated aqueous solutions of Agolin (50 mg/L) and a non-protein nitrogen and mineral solution (uPRO ORANGE [uPRO]; 1.7 mL/L) individually and in combination, where Agolin was added to concentrated uPRO at 3, 4.5, 6% Agolin (w/w) prior to dilution with water at 1.7 mL/L, for a total of five treatments. These were incubated for 48 h with a medium-quality Rhodes grass (Chloris gayana) hay substrate, with gas production, CH4 concentration in gas, and digestibility measured in vitro. In Exp. 2, Droughtmaster steers (n = 24) were fed a basal diet of Rhodes grass hay and were allocated to one of three water treatments (n = 8/treatment) supplemented with either uPRO (2.27 mL uPRO/L water), or one of two inclusion rates of Agolin in combination with uPRO (2.27 mL uPRO and 6 µL Agolin/L water or 2.27 mL uPRO and 24 µL Agolin/L water) with enteric CH4 emissions, feed and water intake, and live weight gain (LWG) measured over 56 d. In Exp. 1, the inclusion of Agolin in uPRO at 6% w/w resulted in a reduction in CH4 production (15.8%; P = 0.003) and the proportion of CH4 in the gas produced (24.5%; P < 0.001). In Exp. 2, steers consuming the lower quantity of Agolin via drinking water had a 16.4% (P = 0.0027) reduction in CH4 production over the experiment, declining from 140 g/d during week 1 to 117 g/d in week 8. This inclusion rate of Agolin in the drinking water also resulted in a 25 g (17.6%) CH4/d decrease in emissions by steers compared to control steers (P = 0.0205). However, no significant differences in CH4 yield (g CH4/kg dry matter intake), or CH4 intensity (g CH4/kg LWG) by steers was observed between treatments. These results demonstrate that Agolin reduces CH4 emissions when mixed in aqueous solution under in vitro and in vivo conditions, providing a potential method to reduce enteric CH4 emissions from cattle in extensive production systems., (© The Author(s) 2024. Published by Oxford University Press on behalf of the American Society of Animal Science.)

A reduction in voluntary feed intake is observed in ruminants consuming nutrient-deficient diets, such as those with a low CP or P content, and has been attributed to active metabolic regulation, rather than a physical constraint. The hypothalamus is the key integrator of feed intake regulation in mammals. The objectives of this experiment were to (1) establish a model of metabolic feed intake regulation in ruminants consuming diets of variable CP and P content, and (2) determine key biochemical pathways and influential points of regulation within the hypothalamus. Merino wethers [n = 40; 23.7 ± 1.4 kg liveweight (mean ± SD)] were fed one of five dietary treatments (n = 8/treatment) for 63 days in individual pens. The treatments included targeted combinations of high (H) and low (L) CP (110 and 55 g/kg DM) and high and low P (2.5 and 0.7 g/kg DM) with 9 MJ metabolisable energy (ME) per kg DM which were fed ad libitum (UMEI; unrestricted ME intake) resulting in four experimental diets (HCP-HP-UMEI, LCP-HP-UMEI, HCP-LP-UMEI and LCP-LP-UMEI). An additional nutritional treatment (HCP-HP-RMEI) restricted intake of the HCP-HP diet to an equivalent ME intake of wethers consuming the LCP-LP-UMEI treatment. Wethers offered the LCP-HP-UMEI, HCP-LP-UMEI and LCP-LP-UMEI treatments consumed 42, 32 and 49% less total DM (P ≤ 0.05), respectively than the HCP-HP-UMEI treatment, and this was not attributable to any physical limitation of the rumen. Plasma concentrations of urea nitrogen and inorganic phosphate indicated that these nutrient deficiencies were successfully established. To assess potential mechanisms, RNA-seq was conducted on samples from the arcuate nucleus (ARC), ventromedial hypothalamus and lateral hypothalamus of the wethers, yielding a total of 301, 8 and 148 differentially expressed genes across all pairwise comparisons, respectively. The expression of NPY, AGRP and CARTPT, known for their regulatory role in mammalian feed intake regulation, had a similar transcriptional response in the ARC of wethers consuming nutrient-deficient treatments and those consuming a ME-restricted treatment, despite these wethers expressing behaviours indicative of satiated and hungry states, respectively. In addition, genes involved with glycolysis (TPI1), the citric acid cycle (CS, OGDH, GLUD1, GOT1) and oxidative phosphorylation (COX5A, ATP5MC1, ATP5F1B, ATP5MC3) were downregulated in the ARC of wethers fed a nutrient deficient (LCP-LP-UMEI) relative to the non-deficient (HCP-HP-UMEI) treatment. In summary, a model for voluntary feed intake restriction was established to determine genome-wide molecular changes in the hypothalamus of young ruminants., (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)

This study investigated the effect of five post-weaning supplementation strategies and two weaning weight groups on long-term growth, puberty and pregnancy percentage of Brahman crossbred heifers. Early-weaned (118 ± 6 kg liveweight) and normally-weaned (183 ± 6 kg liveweight) heifers were allocated to group pens (n = 4 and n = 5/pen for early- and normally-weaned respectively) and offered one of five levels of post-weaning protein supplementation: 0, 1, 2.5, 5 and 10 g of supplement/kg liveweight.day with ad libitum access to a low quality sabi grass (Urochloa mosambicensis) hay during the first dry season (169 days) after weaning. After the post-weaning supplementation period, all heifers grazed the same pastures as a single mob until the end of the experiment and were exposed to fertile bulls from January to May 2016. During the first dry season, supplement intake had a positive linear effect on liveweight gain and hip width gain with no difference in the response between weaning groups. Overall, heifers with higher supplement intakes (i.e. 5 and 10 g/kg) had higher hip height gain (P < 0.005), hip width gain (P < 0.001), body condition score (P < 0.001), and concentration of insulin-like growth factor-1 (P = 0.001), triiodothyronine (P = 0.04) and insulin (P = 0.05) in plasma compared to unsupplemented heifers. These changes resulted in thicker proliferative and hypertrophic zones (both P = 0.03) of the tuber coxae growth plate, larger diameter of terminal hypertrophic chondrocytes (both P = 0.004) at the end of the post-weaning supplementation period when comparing the highest level of supplementation with unsupplemented group. Unsupplemented heifers from both weaning weight groups demonstrated compensatory liveweight gain over the first wet season while evidence of catch-up growth in skeletal dimensions was observed in the second wet season. The main determining factor for pregnancy status of two-year-old Brahman crossbred heifers was pre-mating liveweight (P < 0.001), the pre-mating liveweight was in turn affected by post-weaning supplementation (P = 0.02) or weaning weight group (P < 0.001). This study further demonstrated the positive relationship between premating weight and the occurrence of pregnancy, with an approximate 300 kg pre-mating liveweight required to achieve approximately 80% (67.1-90.3% for a 95% confidence interval) probability of pregnancy in two-year-old Brahman crossbred heifers mated for 4 months., Competing Interests: The authors have declared that no competing interests exist.

Lindsley developed the "say all fast minute every day shuffled" (SAFMEDS) procedure in the late 1970s to enhance the typical use of flash cards (Graf & Auman, 2005). The acronym specifically guides the learner's behavior when using flash cards. A review of SAFMEDS research indicates its successful use with children, college students, and older adults with and without disabilities. The literature also indicates that SAFMEDS procedures are not well documented and have multiple variations, limiting practitioners' ability to know what procedures to use and when. The purpose of this study was to evaluate the effects of a basic SAFMEDS procedure and four supplementary SAFMEDS procedures on the rates of correct and incorrect responding to unfamiliar Russian words and Chinese characters in college students. The results of the study suggest that the basic SAFMEDS procedure produced some learning (i.e., increases in correct responding and decreases in incorrect responding), but all of the supplementary procedures led to greater increases in the number of correct responses per 1-min timing. Further research evaluating differences in performance across the supplementary procedures is warranted., Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest., (© Association for Behavior Analysis International 2021.)

The effects of live weight on carcass characteristics and meat quality of Australian Rangeland goats were determined. Fifty-two intact-male kid goats were fed Mitchell grass hay and finisher pellets ad libitum for 42 days. Prior to slaughter, kids were categorised into live weight groups: 'Heavy' (≈33.1 kg) or 'Light' ≈ 24.3 kg). Fifteen kids per group were randomly selected, slaughtered and carcass characteristics measured. The longissimus lumborum (LL) and the biceps femoris (BF) muscles were removed for quality measurements. The Heavy group had higher dressing, hindquarters, non-carcass component and offal percentages (P < 0.05). Live weight had no effect on proportion of carcass cuts, muscle meat colour, cooking loss, Warner-Bratzler shear force, total fat or ash (P > 0.05), but influenced the moisture and protein content in the LL and the moisture content in the BF (P < 0.05). The findings suggest that live weight of entire male Rangeland goats had no effect on meat quality characteristics., (Crown Copyright © 2021. Published by Elsevier Ltd. All rights reserved.)

In recent years, there has been discussion of the need for training behavior analysts in collaboration skills. There is some consensus that these skills are needed to improve outcomes with clients and with colleagues. Specifically, students of behavior analysis and behavior-analytic practitioners need to learn the skills associated with functioning effectively in interdisciplinary teams. At Melmark, a model of training in this skill set has been developed and integrated into supervision modules for those seeking Board Certified Behavior Analyst certification. In this model, supervisees are exposed to information about the expertise and value of the allied professions of speech-language pathology, occupational therapy, physical therapy, and developmental pediatrics. Rotations are done within these fields to expose trainees to the expertise of these fields and to build skills in collaboration with members of those professions. Trainees are also assigned tasks that require them to identify the contributions of other professions and display appropriate collaborative behavior. Future directions for the model are discussed, including extending it across the organization and developing performance-based assessments and social validity measures., Competing Interests: Conflict of InterestThere are no conflicts of interest or competing interests for any of the authors., (© Association for Behavior Analysis International 2021, corrected publication 2021.)

The objective of this study was to investigate the effect of diet crude protein (CP) content and metabolisable energy (ME) intake on skeletal growth and associated parameters of growing steers prior to and during compensatory growth in weight and catch-up growth in skeletal elongation. The experiment was a factorial design with two cattle genotypes [Brahman crossbred (BX, 178 ± 6 kg) and Holstein-Friesian (HF, 230 ± 34 kg)] and three nutritional treatments; high CP content and high ME intake (HCP-HME), high CP content and low ME intake (HCP-LME) and low CP content and low ME intake (LCP-LME) with the ME intake of HCP-LME matched to that of LCP-LME. Nutritional treatments were imposed over a 103 d period (Phase 1), and after this, all steers were offered ad libitum access to the HCP-HME nutritional treatment for 100 d (Phase 2). Steers fed the high CP content treatment with a low ME intake, showed higher hip height gain (P = 0.04), larger terminal hypertrophic chondrocytes (P = 0.02) and a higher concentration of total triiodothyronine in plasma (P = 0.01) than steers with the same ME intake of the low CP content treatment. In addition, the low CP treatment resulted in significant decreases in bone volume (P = 0.03), bone surface area (P = 0.03) and the concentration of bone-specific alkaline phosphatase in plasma (P < 0.001) compared to steers fed the HCP-HME treatment. A significant interaction between genotype and nutritional treatment existed for the concentration of thyroxine (T4) in plasma where HF steers fed LCP-LME had a lower T4 concentration in plasma (P = 0.05) than BX steers. All steers with a restricted ME intake during Phase 1 demonstrated compensatory growth during Phase 2. However, HF steers fed the LCP treatment during Phase 1 showed a tendency (P = 0.07) for a greater LWG during Phase 2 without any increase in dry matter intake. Results observed at the growth plate and hip height growth suggest that catch-up growth in cattle may also be explained by the growth plate senescence hypothesis. Contrary to our initial hypothesis, the results demonstrate that greater CP intake during ME restriction does not increase compensatory gain in cattle during re-alimentation., Competing Interests: The authors have declared that no competing interests exist.

The field of behavior analysis has defined its scope of practice through credentialing and licensure efforts. However, scope of competence in behavior analysis has received little discussion. Scope of competence refers to activities that the individual practitioner can perform at a certain criterion level (e.g., the functional analysis is conducted accurately and safely, a skill acquisition program includes critical program components and establishes accurate stimulus control). Given the successful efforts of behavior analysts in growth and recognition of the field, it is time for a robust conversation about scope of competence for the field of behavior analysis. This discussion can clarify how behavior analysts self-evaluate their own scope of competence and how they might expand their scope of competence if the needs of consumers require practitioners to expand into new areas., Competing Interests: The authors declare that they have no conflicts of interest.This article does not contain any studies with human participants or animals performed by any of the authors.

Baer, Wolf, and Risley (1968) indicated "technological" was one of seven core dimensions of applied behavior analysis (ABA). They described this dimension as being met if interventions were described well enough to be implemented correctly. Often in the applied settings, a behavior plan is the method by which interventions are communicated to staff and parents for implementation. The necessary components of a behavior plan have been discussed in relation to compliance with regulations (e.g., Vollmer, Iwata, Zarcone, & Rodgers, Research in Developmental Disabilities 13:429-441, 1992), in school settings (e.g., Horner, Sugai, Todd, & Lewis-Palmer, Exceptionality: A Special Education Journal 8:205-215, 2000), and other applied settings (e.g., Tarbox et al., Research in Autism Spectrum Disorder 7:1509-1517, 2013) for the last 25 years. The purpose of this research is to review the literature regarding components of behavior plans and synthesize it with a recent survey of behavior analysts regarding essential components of behavior plans. The results are discussed in light of training, treatment fidelity implications (i.e., Registered Behavior Technician Task List), public policy development (e.g., state initiative for a single behavior plan template), and research opportunities (e.g., comparison of different visual structures)., Competing Interests: The Behavior Analyst Certification Board distributed the survey to all registrants allowing solicitation for no cost. One author, Alissa A. Conway, is part of a team consulting with the state of Michigan to develop a uniform behavior plan template. She is not compensated for her consultation time.The Beacon Services internal review committee reviewed this research protocol and indicated that it did not pose any significant risks to participants.

Behavior analysts likely can evaluate multiple organizations prior to accepting a job due to recent increases in the number of organizations providing ABA services. We argue that evaluating the ethical values of an organization is paramount during the job search process. We provide strategies for evaluating the ethical values of an organization prior to employment and describe considerations from the pre-application process through contract negotiations. Ultimately, we add to the growing body of literature that provides guidance for common problems behavior analysts may face over the course of their careers., Competing Interests: Compliance with Ethical StandardsThe authors declare that they have no conflicts of interest.This article does not contain any studies with human participants or animals performed by any of the authors.

SAFMEDS is an assessment and instructional strategy pioneered in the late 1970s by Ogden Lindsley. SAFMEDS was developed as an extension and improvement of flashcards. The aims of this article are to provide an overview of the literature related to SAFMEDS and to identify further research needs. The results of this review suggest that a great deal of research is still needed to clarify the SAFMEDS procedures and the benefits of SAFMEDS over traditional instruction. These conclusions are in line with broader criticisms of fluency-based instruction., Competing Interests: Conflict of InterestThe authors declare that they have no conflict of interest., (© Association for Behavior Analysis International 2017.)

A series of 3 experiments were conducted to evaluate the use of microalgae as supplements for ruminants consuming low-CP tropical grasses. In Exp. 1, the chemical composition and in vitro protein degradability of 9 algae species and 4 protein supplements were determined. In Exp. 2, rumen function and microbial protein (MCP) production were determined in steers fed speargrass hay alone or supplemented with , , , or cottonseed meal (CSM). In Exp. 3, DMI and ADG were determined in steers fed speargrass hay alone or supplemented with increasing amounts of NPN (urea combined with ammonia sulfate), CSM, or . In Exp. 1, the CP content of and (675 and 580 g/kg DM) was highest among the algae species and higher than the other protein supplements evaluated, and sp. had the highest crude lipid (CL) content (198 g/kg DM). In Exp. 2, supplementation increased speargrass hay intake, the efficiency of MCP production, the fractional outflow rate of digesta from the rumen, the concentration of NHN, and the molar proportion of branched-chain fatty acids in the rumen fluid of steers above all other treatments. acceptance by steers was low and this resulted in no significant difference to unsupplemented steers for all parameters measured for this algae supplement. In Exp. 3, ADG linearly increased with increasing supplementary N intake from both and NPN, with no difference between the 2 supplements. In contrast, ADG quadratically increased with increasing supplementary N intake from CSM. It was concluded that and may potentially be used as protein sources for cattle grazing low-CP pastures.

Maternal feed allowance during pregnancy can affect the development of the ovine placenta and fetus. The impact of variations in feed allowance prior to as well as throughout pregnancy has received less attention. Ewes were offered 0.6 (R), 1.2 (C) or 1.8 (AL) maintenance requirements from 89 days before conception until day 133 of pregnancy. Ewes were euthanised on days 50, 92 and 133 of pregnancy. Ewe live weight and body condition score, maternal and fetal metabolic and hormonal profiles, fetal body dimensions and organ weights, and the number, weight and morphology of placentomes were measured. Maternal live weight and condition score were lower in R compared to AL ewes at all stages of pregnancy (P<0.05). Plasma glucose and albumin concentrations of R ewes were significantly reduced (P<0.05) at mid and late gestation, respectively. Placental components were generally unresponsive to long term variations in maternal feed allowance. However, placental weight was significantly (P<0.05) correlated with fetal weight at days 50 (r=0.59) and 133 (r=0.69) of gestation. By late gestation growth-retarded singleton fetuses from R ewes were 19% lighter (P<0.05), with reduced abdominal (9%) and thoracic (10%) girths (P<0.05) but of similar crown-rump length compared with fetuses from AL ewes. These differences were associated with significantly reduced IGF-I concentrations in fetal plasma (P<0.05). In conclusion, maternal, placental and fetal adaptations to long established planes of variable maternal feed allowance were able to maintain fetal growth during early and mid-pregnancy while fetal growth restriction, associated with reduced fetal IGF-I levels, became apparent in late pregnancy.

The effect of varying short-term maternal feed intake during the peri-conception period on the development of ovine fetal muscle at mid-gestation was investigated. Superovulated donor Merino ewes (n = 24) were fed a roughage/grain pelleted diet (10.1 MJME/kg dry matter) at either 1.5x maintenance (H; high) or 0.5x maintenance (L; low) from 18 days before until 6 days after ovulation. Embryos were transferred to recipient ewes (n = 60) on day 6. Singleton fetuses were collected on day 75 of gestation and placental weights, fetal body dimensions and fetal organ and muscle weights recorded. The number, type and size of muscle fibres and the dry matter, RNA, DNA and protein content in the semitendinosus muscle were determined. Maternal feed intake did not influence body dimensions, organ development or muscle weights in the fetus. However, L feed intake decreased total muscle fibre number in the fetus by approximately 20% (P = 0.06) compared to H feed intake. This resulted from a reduced secondary to primary fibre ratio (P < 0.05) and indicated that secondary fibre formation occurred at a reduced rate in L fetuses. In addition, protein:DNA ratio tended to be lower in muscles of L fetuses (P < 0.1). It is concluded that restricting feed intake over the peri-conception period reduces or delays myogenesis in fetal sheep. The potential mechanisms by which nutritional availability during this period may influence subsequent myogenic development are discussed.

Approximately 480 deaf students (age 10 to 19 years) and 60 hearing students (age eight to 10 years) were asked to judge the grammaticality of sentences containing auxiliary verbs, of sentences where the verb had been deleted, and of sentences in which the verb tense was not marked. The results indicated that deaf students have considerable difficulty with the verb system of English. This difficulty was most pronounced in the formation of tense and voice and in agreement in number and tense. A possible ordering for the acquisition of tense did emerge; from earliest to latest it is: simple past, future, present progressive, perfective, and passive. Improvement in grammaticality judgments appeared to come from an increase in recognition that incorrect sentences were ungrammatical.

Four hundred and twenty-seven deaf students (age 10 to 19 years) and 60 hearing children (age eight to 10 years) judged the grammaticality of sample sentences which contained infinitival or gerundive complements. Results indicated improvement with increasing age for deaf students. Even the youngest hearing students consistently obtained higher scores than most of the deaf students. Although the function of the complement (as subject or object) did not make a difference in performance, the type of complement did. POSS-ing complements were easier than for-to complements. Verb type also influenced performance.

Four groups of deaf subjects between the age of 10-0 and 18-11 years were tested, employing the Test of Syntactic Ability, and the language subtests of the Stanford Achievement Test, in a study of the influence of early language and communication environment on later syntactic language ability. The groups, 18 subjects in each, were dichotomized by whether the parents were hearing or deaf and further subgrouped by the language ability of the parents if the parents were deaf, and by the amount and intensity of oral preschool training provided by the parents if the parents were hearing. The groups were labeled by the type of language used with them in infancy and early childhood: manual English, average manual, intensive oral, and average oral. Results showed significant superiority of the manual English group over the two oral groups on five of the six major test structures of the Test of Syntactic Ability. On the Stanford Achievement Test, the manual English group performed significantly better than the other three groups on all four subtests. The two manual groups performed significantly better than the two oral groups on every test measure employed.

Four hundred and eighty deaf students (age 10 to 18 years) and 60 hearing children (age eight to 10 years) were required to complete a stimulus sentence by selecting the appropriate pronoun from a list provided. The results indicated that subject and object case pronouns were easier than possessive adjectives, which were in turn easier than possessive pronouns and reflexives. Correct use of relative pronouns was the most difficult for both deaf and hearing subjects. Generalizations about the acquisition of the pronoun system must be limited, as it appeared that pronouns are mastered on a pronoun-by-pronoun basis rather than by categories (person, number, case) for both the deaf and hearing subjects.

This longitudinal study investigated the acquisition of question formation in spoken English and American Sign Language (ASL) by two young hearing children of deaf parents. The linguistic environment of the children included varying amounts of exposure and interaction with normal speech and with the nonstandard speech of their deaf parents. This atypical speech environment did not impede the children's acquisition of English question forms. The two children also acquired question forms in ASL that are similar to those produced by deaf children of deaf parents. The two languages, ASL and English, developed in parallel fashion in the two children, and the two systems did not interfere with each other. This dual language development is illustrated by utterances in which the children communicated a sentence in spoken English and ASL simultaneously, with normal English structure in the spoken version and sign language structure in the ASL version.