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In total, 177 of 245 terrestrial carnivores are described as solitary, and much of carnivore ecology is built on the assumptions that interactions between adult solitary carnivores are rare. We employed Global Positioning System (GPS) technology and motion-triggered cameras to test predictions of land-tenure territoriality and the resource dispersion hypothesis in a territorial carnivore, the puma Puma concolor. We documented 89 independent GPS interactions, 60% ofwhich occurred at puma kills (n = 53), 59 camera interactions, 11 (17%) ofwhich captured courtship behaviors, and 5 other interactions (1 F-F, 3 M-F, and 1 M-M). Mean minimum weekly contact rates were 5.5 times higher in winter, the season when elk Cervus elaphus were aggregated at lower elevations and during which puma courtship primarily occurred. In winter, contacts rates were 0.6 ± 0.3 (standard deviation (SD)) interactions/week vs. 0.1 ± 0.1 (SD) interactions/week during summer. The preponderance of interactions at food sources supported the resource dispersion hypothesis, which predicts that resource fluxes can explain temporary social behaviors that do not result in any apparent benefits for the individuals involved. Conspecific tolerance is logical when a prey is so large that the predator that killed it cannot consume it entirely, and thus, the costs of tolerating a conspecific sharing the kill are less than the potential costs associated with defending it and being injured. Puma aggregations at kills numbered as high as 9, emphasizing the need for future research on what explains tolerance among solitary carnivores. [ABSTRACT FROM AUTHOR]

We employed global positioning system (GPS) locations of 18 marked cougars and genetic material from 68 cougars in the Southern Yellowstone Ecosystem to test our current assumptions about the social organization of this non-cooperative, solitary carnivore. We tested whether variable numbers of spatial associations over 7 years of our project could be explained by increasing numbers of wolves in the system, decreasing numbers of prey, changes in cougar density, the mean genetic relatedness between interacting individuals, or the timing of the breeding season. We documented 92 spatial associations and an additional 190 incidents of spatial overlap. Our models suggested only season influenced the number of associations in any given year, and a greater frequency of associations occurred in the breeding season. Nevertheless, the proportion of associations that were male-female (M-F) remained consistent across breeding and non-breeding seasons, suggesting M-F associations may not entirely be driven by mating opportunities; alternatively, the frequency of associations may have been driven by snow depths and the distributions of cougar prey in winter. The mean r value for female-female spatial associations was 0.087 (defined as 'unrelated') and did not significantly differ from M-F or M-M associations. In conclusion, our genetic research revealed not only matrilineal lines in the Southern Yellowstone Ecosystem but also immigration by new females. Subadult males primarily emigrated out of the system, but one male remained philopatric. Our results highlighted the notion that solitary cougars are associating, and potentially interacting, on the landscape with regularity and predictability. Further, our results raised critical questions: If cougars are associating more frequently than previously believed, why do they do so and are these interactions typically agonistic? [ABSTRACT FROM AUTHOR]

We conducted the first long-term and large-scale study of demographic characteristics and reproductive behavior in a wild jaguar (Panthera onca) population. Data were collected through a combination of direct observations and camera trapping on a study area that operates both as a cattle ranch and ecotourism destination. Jaguars exhibited two birth peaks: April/May and October/November, that are the end and the beginning of the wet season in the Pantanal, respectively. The average litter size was 1.43 ± 0.65. Single cubs made up a total of 65.7% of the births, and we found a slight predominance of females (1.15:1 ratio) in litters. The mean age at independence was 17.6 ± 0.98 months, with sex-biased dispersal, with all males (n = 27) leaving the natal home range and 63.6% of females exhibiting philopatry. The interbirth intervals were 21.8 ± 3.2 months and the mean age at first parturition was 31.8 ± 4.2 months. Our results estimated a lifetime reproductive success for female jaguars of 8.13 cubs. Our observations also indicate that female jaguars can display mating behavior during cub rearing or pregnancy, representing 41.4% of the consorts and copulations recorded. We speculate that this behavior has evolved as a defense against infanticide and physical harm to the female. To our knowledge, this is the first time that such behavior is described for this species. All aggressive interactions between females involved the presence of cubs, following the offspring–defense hypothesis, that lead to territoriality among females in mammals, regardless of food availability. In the face of growing threats to this apex predator, this work unveils several aspects of its natural history, representing a baseline for comparison with future research and providing critical information for population viability analysis and conservation planning in the long term. [ABSTRACT FROM AUTHOR]

The ability to non-invasively monitor stress hormone levels in free-ranging animals could significantly aid in conservation and management efforts. Our objective in this study was to demonstrate the effectiveness of assay of fecal corticoid metabolites in detecting a stress response in cougars (Puma concolor). Fecal samples were collected from 9 captive cougars before and after an artificial stressor. Steroid hormones were extracted from the samples. Adrenal corticoid metabolite concentrations of the resulting extracts were quantified using cortisol and corticosterone assays. Results indicated that fecal glucocorticoid metabolite levels increased significantly 24 hours after the stressor in 6 of the 9 individuals. Behavior correlated with the hormonal response; all cougars that displayed a flight response to the stressor also had elevated stress hormone measures. Elevated levels of stress hormones were not observed in individuals that did not attempt to flee. We have demonstrated with this study that measurement of fecal hormone metabolites is sufficiently sensitive to detect an adrenal response to stress in cougars and could be applied in the field to monitor stress levels in free-ranging populations. [ABSTRACT FROM AUTHOR]

Presents information on a study which examined the habitat use and reproductive ecology of the Ocellated Turkey in Tikal National Park, Guatemala. Study area and methods; Results; Discussion.

Humans have been controlling carnivore numbers for centuries. Predator hunting, however, may indirectly influence predator‐prey dynamics unintentionally by influencing the age‐ and sex‐structure of predator populations that exhibit intraspecific (IS) variation in prey selection. We tested for IS in a small population of pumas in the Greater Yellowstone Ecosystem, United States, and identified foraging strategies shared by multiple individuals. Further, we tested extrinsic and intrinsic variables that explained differences in foraging strategy. Our top model was composed of a single intrinsic characteristic, Age. In short, the older the animal, the larger the prey it specialized upon. Our provocative results suggest that the current controversial strategy of increasing puma culling to aid mule deer, as currently underway in Colorado, may in fact exacerbate problems for mule deer by changing the age‐structure of the puma population to predominantly younger animals that are more likely to hunt deer over elk. [ABSTRACT FROM AUTHOR]

Top-down effects of apex predators are modulated by human impacts on community composition and species abundances. Consequently, research supporting top-down effects of apex predators occurs almost entirely within protected areas rather than the multi-use landscapes dominating modern ecosystems. Here, we developed an integrated population model to disentangle the concurrent contributions of a reintroduced apex predator, the grey wolf, human hunting and prey abundances on vital rates and abundance of a subordinate apex predator, the puma. Increasing wolf numbers had strong negative effects on puma fecundity, and subadult and adult survival. Puma survival was also influenced by density dependence. Overall, puma dynamics in our multi-use landscape were more strongly influenced by top-down forces exhibited by a reintroduced apex predator, than by human hunting or bottom-up forces (prey abundance) subsidized by humans. Quantitatively, the average annual impact of human hunting on equilibrium puma abundance was equivalent to the effects of 20 wolves. Historically, wolves may have limited pumas across North America and dictated puma scarcity in systems lacking sufficient refugia to mitigate the effects of competition. [ABSTRACT FROM AUTHOR]

Top-down effects of apex predators are modulated by human impacts on community composition and species abundances. Consequently, research supporting top-down effects of apex predators occurs almost entirely within protected areas rather than the multi-use landscapes dominating modern ecosystems. Here, we developed an integrated population model to disentangle the concurrent contributions of a reintroduced apex predator, the grey wolf, human hunting and prey abundances on vital rates and abundance of a subordinate apex predator, the puma. Increasing wolf numbers had strong negative effects on puma fecundity, and subadult and adult survival. Puma survival was also influenced by density dependence. Overall, puma dynamics in our multi-use landscape were more strongly influenced by top-down forces exhibited by a reintroduced apex predator, than by human hunting or bottom-up forces (prey abundance) subsidized by humans. Quantitatively, the average annual impact of human hunting on equilibrium puma abundance was equivalent to the effects of 20 wolves. Historically, wolves may have limited pumas across North America and dictated puma scarcity in systems lacking sufficient refugia to mitigate the effects of competition. [ABSTRACT FROM AUTHOR]

Camera trapping is the standard field method of monitoring cryptic, low-density mammal populations. Typically, researchers run camera surveys for 60 to 90 days and estimate density using closed population spatially explicit capture-recapture (SCR) models. The SCR models estimate density, capture probability (g0), and a scale parameter (σ) that reflects ranging behaviour. We used a year of camera data from 20 camera stations to estimate the density of male jaguars (Panthera onca) in the Cockscomb Basin Wildlife Sanctuary in Belize, using closed population SCR models. We subsampled the dataset into 276 90-day sessions and 186 180-day sessions. Estimated density fluctuated from 0.51 to 5.30 male jaguars / 100 km2 between the 90-day sessions, with comparatively robust and precise estimates for the 180-day sessions (0.73 to 3.75 male jaguars / 100 km2). We explain the variation in density estimates from the 90-day sessions in terms of temporal variation in social behaviour, specifically male competition and mating events during the three-month wet season. Density estimates from the 90-day sessions varied with σ, but not with the number of individuals detected, suggesting that variation in density was almost fully attributable to changes in estimated ranging behaviour. We found that the models overestimated σ when compared to the mean ranging distance derived from GPS tracking data from two collared individuals in the camera grid. Overestimation of σ when compared to GPS collar data was more pronounced for the 180-day sessions than the 90-day sessions. We conclude that one-off ('snap-shot') short-term, small-scale camera trap surveys do not sufficiently sample wide-ranging large carnivores. When using SCR models to estimate the density from these data, we caution against the use of poor sampling designs and/or misinterpretation of scope of inference. Although the density estimates from one-off, short-term, small-scale camera trap surveys may be statistically accurate within each short-term sampling period, the variation between density estimates from multiple sessions throughout the year illustrate that the estimates obtained should be carefully interpreted and extrapolated, because different factors, such as temporal stochasticity in behaviour of a few individuals, may have strong repercussions on density estimates. Because of temporal variation in behaviour, reliable density estimates will require larger samples of individuals and spatial recaptures than those presented in this study (mean +/- SD = 14.2 +/- 1.2 individuals, 37.7 +/- 4.7 spatial recaptures, N = 276 sessions), which are representative of, or higher than published sample sizes. To satisfy the need for larger samples, camera surveys will need to be more expansive with a higher density of stations. In the absence of this, we advocate longer sampling periods and subsampling through time as a means of understanding and describing stability or variation between density estimates. [ABSTRACT FROM AUTHOR]

Summary: We tested for plague (Yersinia pestis) in a puma population in the Greater Yellowstone Ecosystem (GYE) over 9 years, overlapping a case when a boy in the area became infected with plague. Antibodies to Y. pestis were detected in 8 of 17 (47%) pumas tested by complement-enzyme-linked immunosorbent assay, and the organism itself was detected in 4 of 11 (36%) pumas tested after necropsy. Neither puma sex nor age was significantly associated with Y. pestis exposure or mortality, although our sample size was small. The overall prevalence of exposure we recorded was similar to that found along the western slope of Colorado, which is adjacent to the Four Corners region, a known plague hotspot in the USA. This suggests that: (1) Y. pestis may be present at higher levels in the GYE than previously assumed; (2) plague is a significant source of mortality for local pumas (6.6% of sub-adult and adult mortality); and (3) pumas may be a useful sentinel for potential risk of plague exposure to humans throughout the West. We would also emphasize that hunters and others handling pumas in this region should be made aware of the possibility of exposure. [ABSTRACT FROM AUTHOR]

Reflects on the joint Russia-United States Siberian Tiger conservation project in Russian Far East, Russia. Aim of the project; Threat facing Siberian tigers; Obstacles faced by the project; Knowledge gained from the marking the tigers.

Abstract: Humans are primary drivers of declining abundances and extirpation of large carnivores worldwide. Management interventions to restore biodiversity patterns, however, include carnivore reintroductions, despite the many unresolved ecological consequences associated with such efforts. Using multistate capture–mark–recapture models, we explored age‐specific survival and cause‐specific mortality rates for 134 pumas (Puma concolor) monitored in the Greater Yellowstone Ecosystem during gray wolf (Canis lupus) recovery. We identified two top models explaining differences in puma survivorship, and our results suggested three management interventions (unsustainable puma hunting, reduction in a primary prey, and reintroduction of a dominant competitor) have unintentionally impacted puma survival. Specifically, puma survival across age classes was lower in the 6‐month hunting season than the 6‐month nonhunting season; human‐caused mortality rates for juveniles and adults, and predation rates on puma kittens, were higher in the hunting season. Predation on puma kittens, and starvation rates for all pumas, also increased as managers reduced elk (Cervus elaphus) abundance in the system, highlighting direct and indirect effects of competition between recovering wolves and pumas over prey. Our results emphasize the importance of understanding the synergistic effects of existing management strategies and the recovery of large, dominant carnivores to effectively conserve subordinate, hunted carnivores in human‐dominated landscapes. [ABSTRACT FROM AUTHOR]

Protected areas serve as population strongholds for many large carnivores, with multi‐use landscapes along their borders forming the front‐lines of wildlife conservation. Understanding large carnivore population dynamics within working landscapes is difficult where anthropogenic mortality is high and unregulated. This study focused on working ranches, where killing jaguars (Panthera onca) and their prey was prohibited, to gain insight into jaguar population potential across multi‐use landscapes. Faced with forest fragmentation, presence of domestic livestock, and dynamic land‐use practices, we expected jaguar populations in working landscapes to be predominantly male and transient, with low cub production, and inflated population densities in remnant forest patches, versus protected areas where we expected native forest habitat and stable jaguar territories. Using camera traps and spatial‐capture recapture analyses, we observed that male jaguars demonstrated larger‐scale movements and were more detectable than females (0.07 ± 0.01 SE vs. 0.02 ± 0.01 SE) in both working and protected landscapes. Female jaguars in ranches traveled farther than females in parks. Carnivore density increased with forest cover and wild prey activity, decreased with domestic prey activity, and was marginally higher in ranches (4.08 individuals/100 km2 ± 0.73 SE) than in parks (3.59 individuals/100 km2 ± 0.64 SE). Females outnumbered males in both landscapes (2.20–2.60 females/100 km2 vs. ~1.60 males/100 km2), although local male density reached up to 11.00 males/100 km2 in ranches (vs. 3.50 males/100 km2 in parks). While overall jaguar density was patchier in protected areas (x̿ = 0.69 parks, 0.54 ranches), inter‐annual patchiness was higher within ranches (Moran's I = 0.49–0.60 ranches, 0.69–0.70 parks), reflecting changes in cattle management. Despite major habitat alterations, working landscapes can support carnivore densities equivalent to (or exceeding that of) unmodified forest habitat, provided that wildlife‐tolerant ranching practices are maintained. [ABSTRACT FROM AUTHOR]

Presents an article on the Jaguar Conservation Program of the Wildlife Conservation Society in Sierra Madre, Mexico. Protection of the natural heritage of jaguars; Utilization of heat-sensitive trip-cameras for the study of jaguars; Commitment to the protection of the jaguar ecology.

There are several alternative hypotheses about the effects of territoriality, kinship and prey availability on individual carnivore distributions within populations. The first is the land-tenure hypothesis, which predicts that carnivores regulate their density through territoriality and temporal avoidance. The second is the kinship hypothesis, which predicts related individuals will be clumped within populations, and the third is the resource dispersion hypothesis, which suggests that resource richness may explain variable sociality, spatial overlap or temporary aggregations of conspecifics., Research on the socio-spatial organization of animals is essential in understanding territoriality, intra- and interspecific competition, and contact rates that influence diverse ecology, including disease transmission between conspecifics and courtship behaviours., We explored these hypotheses with data collected on a solitary carnivore, the cougar ( Puma concolor), from 2005 to 2012 in the Southern Yellowstone Ecosystem, Wyoming, USA. We employed 27 annual home ranges for 13 cougars to test whether home range overlap was better explained by land tenure, kinship, resource dispersion or some combination of the three., We found support for both the land tenure and resource dispersion hypotheses, but not for kinship. Cougar sex was the primary driver explaining variation in home range overlap. Males overlapped significantly with females, whereas the remaining dyads (F-F, M-M) overlapped significantly less. In support for the resource dispersion hypothesis, hunting opportunity (the probability of a cougar killing prey in a given location) was often higher in overlapping than in non-overlapping portions of cougar home ranges. In particular, winter hunt opportunity rather than summer hunt opportunity was higher in overlapping portions of female-female and male-female home ranges., Our results may indicate that solitary carnivores are more tolerant of sharing key resources with unrelated conspecifics than previously believed, or at least during periods of high resource availability. Further, our results suggest that the resource dispersion hypothesis, which is typically applied to social species, is applicable in describing the spatial organization of solitary carnivores. [ABSTRACT FROM AUTHOR]

Conservation of carnivores involves finding solutions to minimize habitat loss and human-wildlife conflict. Understanding the nature of land-use economics can allow us to mitigate both threats. In the Pantanal, the two main economic activities are cattle ranching and ecotourism, each of which directly and indirectly affect the persistence of jaguars (Panthera onca). To understand how the geography of these economic activities is related to jaguar populations, we developed a jaguar distribution model (JDM), livestock density model, and ecotourism lodge density model for the Pantanal. Due to the recent wildfires within the Pantanal, we also assess the impact of burnt areas that are suitable for jaguars, cattle ranching, and tourism. Our JDM indicate that 64% of the Pantanal holds suitable habitat for jaguars. However, jaguar habitat suitability was positively correlated with ecotourism, but negatively correlated with areas most suitable for intensive cattle-ranching. This demonstrates a biome-wide scenario compatible with jaguar conservation. Of particular concern, recent wildfires overlap most suitable areas for jaguars. If wildfires become increasingly frequent, this would represent a serious threat to jaguars and many other wildlife populations. We emphasize the global importance of the Pantanal wetland ecoregion as a key stronghold for long-term jaguar conservation. [ABSTRACT FROM AUTHOR]

We assessed whether use of 2 methods, intensive very high frequency (VHF) radiotelemetry and Global Positioning System (GPS) cluster sampling, yielded similar estimates of cougar (Puma concolor) kill rates in Yellowstone National Park, 1998-2005. We additionally determined biases (underestimation or overestimation of rates) resulting from each method. We used modeling to evaluate what characteristics of clusters best predicted a kill versus no kill and further evaluated which predictor(s) minimized effort and the number of missed kills. We conducted 16 VHF ground predation sequences resulting in 37 kill intervals (KIs) and 21 GPS sequences resulting in 84 KIs on 6 solitary adult females, 4 maternal females, and 5 adult males. Kill rates (days/kill and biomass [kg] killed/day) did not differ between VHF and GPS predation sampling methods for maternal females, solitary adult females, and adult males. Sixteen of 142 (11.3%) kills detected via GPS clusters were missed through VHF ground-based sampling, and the kill rate was underestimated by an average of 5.2 (95% CI = 3.8-6.6) days/kill over all cougar social classes. Five of 142 (3.5%) kills identified by GPS cluster sampling were incorrectly identified as the focal individual's kill from scavenging, and the kill rate was overestimated within the adult male social class by an average of 5.8 (95% CI = 3.0-8.5) days/ungulate kill. The number of nights (locations between 2000 hours and 0500 hours) a cougar spent at a cluster was the most efficient variable at predicting predation, minimizing the missed kills, and minimizing number of extra clusters that needed to be searched. In Yellowstone National Park, where competing carnivores displaced cougars from their kills, it was necessary to search extra sites where a kill may not have been present to ensure we did not miss small, ungulate prey kills or kills with displacement. Using predictions from models to assign unvisited clusters as no kill, small prey kill, or large prey kill can bias downward the number of kills a cougar made and bias upward kills made by competitors that displace cougars or scavenge cougar kills. Our findings emphasize that field visitation is crucial in determining displacement and scavenging events that can result in biases when using GPS cluster methods in multicarnivore systems. [ABSTRACT FROM AUTHOR]

In a companion article (Beier et al. 2006), we identified 2 sets of unreliable inferences that may compromise efforts to conserve the Florida panther (Puma concolor coryi). In spite of serious flaws in methodology and interpretation, these unreliable conclusions have appeared in prominent, peer-refereed scientific journals and have been repeatedly cited and miscited in support of panther conservation. Future editors and referees may reduce these errors by insisting on adherence to an Introduction, Methods, Results, and Discussion (IMRAD) format; checking improbable assertions attributed to earlier papers; and refusing to allow scientific inference in publication formats not subject to scientific peer review (e.g., editorials). We urge conservation biologists to view science as an adaptive process and to use the method of multiple working hypotheses (Chamberlin 1890) that are now a central feature of adaptive resource management (Walters 1986, Williams et al. 2002). We advocate a workshop approach, similar to that used for analysis of data for the northern spotted owl (Strix occidentalis; Anderson et al. 1999), to deal with scientific disagreement where, as in the case with panthers, stakeholders have entrenched points of view. Finally, we recommend the creation of an independent Scientific Steering Committee to address long-term issues of future research and monitoring of Florida panthers. [ABSTRACT FROM AUTHOR]

Abstract Leopards, Panthera pardus, are widely distributed across southern Asia and sub-Saharan Africa. The extent and phylogeographic patterns of molecular genetic diversity were addressed in a survey of 77 leopards from known geographical locales representing 13 of the 27 classical trinomial subspecies. Phylogenetic analysis of mitochondrial DNA sequences (727 bp of NADH5 and control region) and 25 polymorphic microsatellite loci revealed abundant diversity that could be partitioned into a minimum of nine discrete populations, tentatively named here as revised subspecies: P. pardus pardus, P. p. nimr, P. p. saxicolor, P. p. fusca, P. p. kotiya, P. p. delacouri, P. p. japonensis, P. p. orientalis and P. p. melas. However, because of limited sampling of African populations, this may be an underestimate of modern phylogeographic population structure. Combined phylogeographic and population diversity estimates support an origin for modern leopard lineages 470 000–825 000 years ago in Africa followed by their migration into and across Asia more recently (170 000–300 000 years ago). Recent demographic reductions likely have led to genetic impoverishment in P. p. orientalis and in the island subspecies P. p. kotiya. [ABSTRACT FROM AUTHOR]

Ecosystem engineers create physical changes in abiotic and biotic material, and through this process control the availability of resources for other species. Predators that abandon large portions of their prey may be ecosystem engineers that create habitat for carrion-dependent invertebrates that utilize carcasses during critical life-history periods. Between 04-May-2016 and 04-Oct-2016, we sampled beetle assemblages at 18 carcasses of prey killed by pumas and matching control sites in the southern Greater Yellowstone Ecosystem, USA, to measure the extent to which beetle families utilized these carcass "habitats". We used generalized linear-mixed models and linear-mixed effect models to examine changes in beetle abundance, species richness, and Simpson's Index of Diversity. We estimated kill rates and carrion production rates for individual pumas to better assess the impact of pumas on invertebrate communities. We collected 24,209 beetles representing 215 species. We identified eight beetle families that had significantly higher abundance at carcasses than control sites. Carcasses had a statistically large to very large effect (determined using Cohen's d) on beetle abundance, richness, and diversity for the initial 8 weeks of sampling. Our research revealed strong effects of an ecosystem engineer on beetle assemblages while highlighting the potential role of apex predators in creating and modifying physical habitats for carrion-dependent species. This suggests that there may be consequences for invertebrate communities where apex predators exist at reduced numbers or have been eradicated. The ecological role of invertebrates is often overlooked, yet they are essential taxa that provide critical ecological services upon which we depend. [ABSTRACT FROM AUTHOR]

Abstract: Evaluating range‐wide habitat use by a target species requires information on species occurrence over broad geographic regions, a process made difficult by species rarity, large spatiotemporal sampling domains and imperfect detection. We address these challenges in an assessment of habitat use for jaguars (Panthera onca) outside protected areas in Central America. Occurrence records were acquired within 12 putative corridors using interviews with knowledgeable corridor residents. We developed a Bayesian hierarchical occupancy model to gain robust inference, allowing for heterogeneity introduced in the sampling process over space and time, using records of jaguar occurrence prone to false positives and false negatives. Probability of false detection of jaguars increased with the number of interviews conducted per unit (from 5.42% to 7.74% given <4 and ≥4 observers per unit). True probability of detection (p = 0.58) increased with the number of days interviewees spent in a survey unit per year. Failing to account for false positives biased predicted habitat use high (c. 1.8×), especially where occurrence records were sparse. Probability of site use by jaguars increased with greater forest cover, prey richness, and distance from human settlements, and decreased with greater agricultural cover, elevation, and distance from protected areas. Site use probabilities averaged 0.15–0.97 by corridor, providing relatively fine‐scale resolution of predicted jaguar occurrence consistent with known patterns of jaguar gene flow across Central America. Model validation, accounting for both false positives and negatives in the observation process, indicated moderate correspondence between model‐predicted observations and actual observations for withheld data (0.65, 95% CRI 0.59–0.71), with sensitivity and specificity rates of 0.69 (0.61–0.77) and 0.59 (0.50–0.68), respectively. These results demonstrate that reliable predictions can be achieved despite the complexity of large‐scale, interview‐based analyses of species occurrence. Synthesis and applications. Our Bayesian hierarchical occupancy model accommodated heterogeneity caused by typical sampling inequities and idiosyncrasies associated with interview data, yielding robust estimates of jaguar habitat use. Our approach is applicable to any wide‐ranging and readily identifiable species and has particular utility for rare species in human‐dominated landscapes where traditional survey techniques (e.g. camera traps) may be impractical. [ABSTRACT FROM AUTHOR]

Carrion promotes biodiversity and ecosystem stability, and large carnivores provide this resource throughout the year. In particular, apex felids subordinate to other carnivores contribute more carrion to ecological communities than other predators. We measured vertebrate scavenger diversity at puma ( Puma concolor ) kills in the Greater Yellowstone Ecosystem, and utilized a model-comparison approach to determine what variables influenced scavenger diversity (Shannon's H ) at carcasses. We documented the highest vertebrate scavenger diversity of any study to date (39 birds and mammals). Scavengers represented 10.9% of local birds and 28.3% of local mammals, emphasizing the diversity of food-web vectors supported by pumas, and the positive contributions of pumas and potentially other subordinate, apex felids to ecological stability. Scavenger diversity at carcasses was most influenced by the length of time the carcass was sampled, and the biological variables, temperature and prey weight . Nevertheless, diversity was relatively consistent across carcasses. We also identified six additional stalk-and-ambush carnivores weighing > 20 kg, that feed on prey larger than themselves, and are subordinate to other predators. Together with pumas, these seven felids may provide distinctive ecological functions through their disproportionate production of carrion and subsequent contributions to biodiversity. We urge conservation managers to increase support for these species, as a means of prioritizing resources to best ensure the persistence of carrion in natural systems. [ABSTRACT FROM AUTHOR]

The article focuses on a study regarding adaptive social strategies in a solitary carnivore. It mentions that cost-benefit trade-offs for individuals participating in social behaviors are the basis for current theories on the evolution of social behaviors and societies. It also mentions the use of network analysis of conspecific tolerance at food sources to provide evidence that a solitary carnivore.

Much of our understanding of the effects top predators play in structuring ecological communities are from studies documenting ecological changes following the recovery or reintroduction of large carnivores. Reintroduced predators, for example, may create unanticipated competition scenarios that influence local carnivore guilds. Here, we tested whether newly released Mexican gray wolves in Chihuahua, Mexico interacted with resident pumas, as a first step in exploring potential competition between the two species. We employed GPS data and novel methods offered by MoveMine 2.0 to quantify attraction-repulsion between individual wolves and pumas during two time periods: the first when wolves were free roaming, and the second when they restricted their movements to an area around their den and rendezvous site to protect and provide for pups. In summary, attraction-repulsion analyses conducted with MoveMine provided meaningful outputs, but we would highlight the importance of fieldwork to interpret interactions and significance values calculated between pairs. We found that high attraction values resulted from mutual attraction between wolves in the same pack, between wolves and one puma they repeatedly harassed, as well as between two pumas that repeatedly exhibited social tolerance for each other. Thus, the analyses offered by MoveMine provided a powerful means of identifying interactions and assessing attraction-repulsion relevant to questions of intra- and interspecific competition, but required intimate knowledge of the species studied and the interactions between them to interpret outputs correctly. [ABSTRACT FROM AUTHOR]

In this study, we estimate life history parameters and abundance for a protected jaguar population using camera-trap data from a 14-year monitoring program (2002–2015) in Belize, Central America. We investigated the dynamics of this jaguar population using 3,075 detection events of 105 individual adult jaguars. Using robust design open population models, we estimated apparent survival and temporary emigration and investigated individual heterogeneity in detection rates across years. Survival probability was high and constant among the years for both sexes (φ = 0.78), and the maximum (conservative) age recorded was 14 years. Temporary emigration rate for the population was random, but constant through time at 0.20 per year. Detection probability varied between sexes, and among years and individuals. Heterogeneity in detection took the form of a dichotomy for males: those with consistently high detection rates, and those with low, sporadic detection rates, suggesting a relatively stable population of ‘residents’ consistently present and a fluctuating layer of ‘transients’. Female detection was always low and sporadic. On average, twice as many males than females were detected per survey, and individual detection rates were significantly higher for males. We attribute sex-based differences in detection to biases resulting from social variation in trail-walking behaviour. The number of individual females detected increased when the survey period was extended from 3 months to a full year. Due to the low detection rates of females and the variable ‘transient’ male subpopulation, annual abundance estimates based on 3-month surveys had low precision. To estimate survival and monitor population changes in elusive, wide-ranging, low-density species, we recommend repeated surveys over multiple years; and suggest that continuous monitoring over multiple years yields even further insight into population dynamics of elusive predator populations. [ABSTRACT FROM AUTHOR]

Olfactory communication among mammals remains poorly studied yet may be key to understanding their ecology. This is particularly true for mammalian carnivores, which rely extensively on scent marking for communication. Previous research suggests that carnivore latrines play a large role in both intra- and inter-specific communication. Despite the apparent complexity of mammal use of latrines, little work has examined behavior patterns of species that visit latrines. We used motion-triggered video cameras to study use and behavior of mammals at ocelot (Leopardus pardalis) latrines in Costa Rica. We documented temporal patterns of use by the focal species (ocelots), diversity of mammalian species using latrines, and behaviors that occurred at these sites. Ocelots showed peaks in visitation every 60-67 days and a shorter gap between intersexual versus intrasexual visits, supporting the idea that ocelot latrines are used to communicate information about reproductive status. Fourteen terrestrial mammal species visited the latrines, and these species engaged in a variety of behaviors, including mark investigation, scent marking, and acceptance of scent marks. The complexity and frequency of behaviors by nonfocal species suggest that latrines may play as important a role in communication for these other species as they do for ocelots. [ABSTRACT FROM AUTHOR]

Infanticide is an antagonistic behavior that may provide an evolutionary benefit for the perpetrator. Cases of infanticide have rarely been reported in Neotropical carnivores. The objective of this study was to provide empirical evidence of infanticide in a local jaguar ( Panthera onca) population in the Brazilian Pantanal. We present infanticide data from opportunistic sampling. Each month, from 2013 to 2015, we monitored pastures for evidence of livestock carcasses. A remotely triggered camera trap was placed at encountered fresh cattle carcasses. Through this monitoring, two cases of infanticide were recorded. Both cases occurred in close proximity to a cattle carcass, a large prey item that may attract multiple jaguars-including females with cubs around age of weaning-and increase the risk of infanticide. Our observations provide new empirical evidence of jaguar social behavior and intraspecific competitive interactions, potentially driven by locally high population density and resource provision. [ABSTRACT FROM AUTHOR]

Much of our understanding of the effects top predators play in structuring ecological communities are from studies documenting ecological changes following the recovery or reintroduction of large carnivores. Reintroduced predators, for example, may create unanticipated competition scenarios that influence local carnivore guilds. Here, we tested whether newly released Mexican gray wolves in Chihuahua, Mexico interacted with resident pumas, as a first step in exploring potential competition between the two species. We employed GPS data and novel methods offered by MoveMine 2.0 to quantify attraction-repulsion between individual wolves and pumas during two time periods: the first when wolves were free roaming, and the second when they restricted their movements to an area around their den and rendezvous site to protect and provide for pups. In summary, attraction-repulsion analyses conducted with MoveMine provided meaningful outputs, but we would highlight the importance of fieldwork to interpret interactions and significance values calculated between pairs. We found that high attraction values resulted from mutual attraction between wolves in the same pack, between wolves and one puma they repeatedly harassed, as well as between two pumas that repeatedly exhibited social tolerance for each other. Thus, the analyses offered by MoveMine provided a powerful means of identifying interactions and assessing attraction-repulsion relevant to questions of intra- and interspecific competition, but required intimate knowledge of the species studied and the interactions between them to interpret outputs correctly. [ABSTRACT FROM AUTHOR]

The impact of extensive changes in land use and climate on species has led to an increasing focus on large-scale conservation planning. However, these plans are often static conservation prescriptions set against a backdrop of rapidly changing environments, which suggests that large-scale information on threats can improve the functionality of planning efforts. Jaguars ( Panthera onca ) are the focus of a range-wide conservation strategy extending from Mexico to Argentina that consists of jaguar conservation units (JCUs) and modeled corridors. Recent deforestation is a major threat to jaguar populations, but forest loss has not been systematically assessed across the entire jaguar network. In this study, we quantified the amount and rate of deforestation in JCUs and corridors between 2000 and 2012. JCUs lost 37,780 km 2 forest (0.93%) at an increasing rate of 149.2 km 2 yr −2 , corridors lost 45,979 km 2 (4.43%) at a decreasing rate of 40.1 km 2 yr −2 , and levels of forest fragmentation increased in corridors. Protected sections of JCUs and corridors lost less forest than unprotected sections, suggesting efforts to increase protected status of jaguar conservation areas are warranted. Higher deforestation in corridors indicates difficulties in maintaining connectivity of jaguar populations, and suggests the need for increased engagement with communities within corridor landscapes. Assessment of spatial variability of anthropogenic threats within the jaguar network may improve jaguar conservation by informing network prioritization and function. [ABSTRACT FROM AUTHOR]

Mesoamerican jaguars (Panthera onca) have been extirpated from over 77% of their historic range, inhabiting fragmented landscapes at potentially reduced population sizes. Maintaining and restoring genetic diversity and connectivity across human-altered landscapes has become a major conservation priority; nonetheless large-scale genetic monitoring of natural populations is rare. This is the first regional conservation genetic study of jaguars to primarily use fecal samples collected in the wild across five Mesoamerican countries: Belize, Costa Rica, Guatemala, Honduras, and Mexico. We genotyped 445 jaguar fecal samples and examined patterns of genetic diversity and connectivity among 115 individual jaguars using data from 12 microsatellite loci. Overall, moderate levels of genetic variation were detected (NA = 4.50 ± 1.05, AR = 3.43 ± 0.22, HE = 0.59 ± 0.04), with Mexico having the lowest genetic diversity, followed by Honduras, Guatemala, Belize, and Costa Rica. Population-based gene flow measures (FST = 0.09 to 0.15, Dest = 0.09 to 0.21), principal component analysis, and Bayesian clustering applied in a hierarchical framework revealed significant genetic structure in Mesoamerican jaguars, roughly grouping individuals into four genetic clusters with varying levels of admixture. Gene flow was highest among Selva Maya jaguars (northern Guatemala and central Belize), whereas genetic differentiation among all other sampling sites was moderate. Genetic subdivision was most pronounced between Selva Maya and Honduran jaguars, suggesting limited jaguar movement between these close geographic regions and ultimately refuting the hypothesis of contemporary panmixia. To maintain a critical linkage for jaguars dispersing through the Mesoamerican landscape and ensure long-term viability of this near threatened species, we recommend continued management and maintenance of jaguar corridors. The baseline genetic data provided by this study underscores the importance of understanding levels of genetic diversity and connectivity to making informed management and conservation decisions with the goal to maintain functional connectivity across the region. [ABSTRACT FROM AUTHOR]

Prey selection is exhibited by predator populations that kill a prey species disproportionate to its availability, or alternatively, individual predators that select prey disproportionate to the mean selection exhibited by their populations. Prey selection is a simple calculation when one can determine prey availability; however, measuring prey availability is challenging. We compared population- and individual-level prey selection as determined with two measures of prey availability for five ungulate species killed by pumas ( Puma concolor (L., 1771)) in the Southern Yellowstone Ecosystem, USA: (1) annual prey counts and (2) total prey killed by marked pumas. We also tested whether individual pumas in the population exhibited a narrower dietary niche breadth compared with their population as a whole. The two methods yielded different estimates of prey availability and highlighted the need to consciously match prey availability estimates with appropriate ecological questions. Prey counts may have overestimated elk ( Cervus canadensis (Erxleben, 1777)) abundance and underestimated deer abundance, whereas predation data may have better captured the influence of prey size on puma-specific prey vulnerability and availability. Prey counts were the more appropriate metric for analyzing population-level prey selection or differences in interspecific foraging, whereas total prey killed was the more appropriate metric for intraspecific comparisons. [ABSTRACT FROM AUTHOR]

Background: The agents of spotted fevers in Latin America are Rickettsia rickettsii, R. parkeri, Rickettsia sp. strain Atlantic rainforest, and R. massiliae. In Continental Central America, R. rickettsii remains the only known pathogenic tick-borne rickettsia. In the present study, ticks were collected from wild mammals in natural areas of Belize. Besides providing new data of ticks from Belize, we investigated rickettsial infection in some of these ticks. Our results provide ticks harboring rickettsial agents for the first time in Central America. Methods: Between 2010 and 2015, wild mammals were lived-trapped in the tropical broadleaf moist forests of central and southern Belize. Ticks were collected from the animals and identified to species by morphological and molecular analysis (DNA sequence of the tick mitochondrial 16S RNA gene). Some of the ticks were tested for rickettsial infection by molecular methods (DNA sequences of the rickettsial gltA and ompA genes). Results: A total of 84 ticks were collected from 8 individual hosts, as follows: Amblyomma pacae from 3 Cuniculus paca; Amblyomma ovale and Amblyomma coelebs from a Nasua narica; A. ovale from an Eira Barbara; A. ovale, Amblyomma cf. oblongoguttatum, and Ixodes affinis from a Puma concolor; and A. ovale, A. coelebs, A. cf. oblongoguttatum, and I. affinis from two Panthera onca. Three rickettsial agents were detected: Rickettsia amblyommii in A. pacae, Rickettsia sp. strain Atlantic rainforest in A. ovale, and Rickettsia sp. endosymbiont in Ixodes affinis. Conclusions: The present study provides unprecedented records of ticks harboring rickettsial agents in the New World. An emerging rickettsial pathogen of South America, Rickettsia sp. strain Atlantic rainforest, is reported for the first time in Central America. Besides expanding the distribution of 3 rickettsial agents in Central America, our results highlight the possible occurrence of Rickettsia sp. strain Atlantic rainforest-caused spotted fever human cases in Belize, since its possible vector, A. ovale, is recognized as one of the most important human-biting ticks in the Neotropical region. [ABSTRACT FROM AUTHOR]

Cougars (Puma concolor) are widely distributed throughout the western portion of North America and are generally described as solitary carnivores. Most cougar social interactions have been described as instances of parental care, intraspecific strife, or breeding. We report an apparent case of an adoption of orphaned juveniles in a wild cougar population. We used radiotelemetry and direct visual observations to document an adult female, her 3 dependent offspring, and 2 orphaned juvenile males physically interacting, sharing bed sites, and sharing kills in the late winter of 2007/2008. We consider the potential benefits and/or negative effects of these social interactions, and the role that relatedness and/or familiarity may play in the motivation for developing such associations. [ABSTRACT FROM AUTHOR]

Abstract: An understanding of how stressors affect dispersal attributes and the contribution of local populations to multi-population dynamics are of immediate value to basic and applied ecology. Puma (Puma concolor) populations are expected to be influenced by inter-population movements and susceptible to human-induced source–sink dynamics. Using long-term datasets we quantified the contribution of two puma populations to operationally define them as sources or sinks. The puma population in the Northern Greater Yellowstone Ecosystem (NGYE) was largely insulated from human-induced mortality by Yellowstone National Park. Pumas in the western Montana Garnet Mountain system were exposed to greater human-induced mortality, which changed over the study due to the closure of a 915km2 area to hunting. The NGYE’s population growth depended on inter-population movements, as did its ability to act as a source to the larger region. The heavily hunted Garnet area was a sink with a declining population until the hunting closure, after which it became a source with positive intrinsic growth and a 16× increase in emigration. We also examined the spatial and temporal characteristics of individual dispersal attributes (emigration, dispersal distance, establishment success) of subadult pumas (N =126). Human-caused mortality was found to negatively impact all three dispersal components. Our results demonstrate the influence of human-induced mortality on not only within population vital rates, but also inter-population vital rates, affecting the magnitude and mechanisms of local population’s contribution to the larger metapopulation. [Copyright &y& Elsevier]

We studied survival and causes of mortality of radiocollared cougars (Puma concolor) on the Greater Yellowstone Northern Range (GYNR) prior to (1987-1994) and after wolf (Canis lupus) reintroduction (1998-2005) and evaluated temporal, spatial, and environmental factors that explain variation in adult, subadult, and kitten survival. Using Program MARK and multimodel inference, we modeled cougar survival based on demographic status, season, and landscape attributes. Our best models for adult and independent subadults indicated that females survived better than males and survival increased with age until cougars reached older ages. Lower elevations and increasing density of roads, particularly in areas open to cougar hunting north of Yellowstone National Park (YNP), increased mortality risks for cougars on the GYNR. Indices of ungulate biomass, cougar and wolf population size, winter severity, rainfall, and individual characteristics such as the presence of dependent young, age class, and use of Park or Wilderness were not important predictors of survival. Kitten survival increased with age, was lower during winter, increased with increasing minimum estimates of elk calf biomass, and increased with increasing density of adult male cougars. Using our best model, we mapped adult cougar survival on the GYNR landscape. Results of receiver operating characteristic (ROC) analysis indicated a good model fit for both female (area under the curve [AUC] = 0.81, 95%CI = 0.70-0.92, n = 35 locations) and male cougars (AUC = 0.84, 95%CI = 0.74- 0.94, n = 49 locations) relative to hunter harvest locations in our study area. Using minimum estimates of survival necessary to sustain the study population, we developed a source-sink surface and we identify several measures that resource management agencies can take to enhance cougar population management based on a source-sink strategy. [ABSTRACT FROM AUTHOR]

Conventional methods for monitoring cougar, Puma concolor, populations involve capture, tagging, and radio-collaring, but these methods are time-consuming, expensive, and logistically challenging. For difficult-to-study species such as cougars, noninvasive genetic sampling (NGS) may be a useful alternative. The ability to identify individuals from samples collected through NGS methods provides many opportunities for developing population-monitoring tools, but the utility of these survey methods is dependent upon collection of samples and accurate genotyping of those samples. In January 2003, we initiated a 3-yr evaluation of NGS methods for cougars using a radio-collared population in Yellowstone National Park (YNP), USA. Our goals were to: 1) determine which DNA collection method, hair snares or snow tracking, provided a better method for obtaining samples for genetic analysis, 2) evaluate reliability of the genetic data derived from hair samples collected in the field, and 3) evaluate the potential of NGS for demographic monitoring of cougar populations. Snow tracking yielded more hair samples and was more cost effective than snagging hair with rub pads. Samples collected from bed sites and natural hair snags (e.g., branch tips, thorn bushes) while snow tracking accurately identified and sexed 22 individuals (9 F, 13 M). The ratio of the count from snow tracking to the count from radio-telemetry was 15:24 in winter 2004, 13:12 in 2005, and 22:29 for both years combined. Annual capture probabilities for obtaining DNA from snow tracking varied considerably between years for females (0.42 in 2004 and 0.88 in 2005) but were more consistent for males (0.77 in 2004 and 0.88 in 2005). Our results indicate that snow tracking can be an efficient, reliable NGS method for cougars in YNP and has potential for estimating demographic and genetic parameters of other carnivore populations in similar climates. [ABSTRACT FROM AUTHOR]

Spacing patterns of large carnivores can affect demographic parameters of populations that, in turn, influence effective population size. As a result, better understanding of spatial structure can provide insight into effective conservation strategies. We examined home-range size, spacing characteristics, and changes in land tenure of radiocollared Amur tigers (Panthera tigris altaica) on the Sikhote-Alin Biosphere Zapovednik, Russia, from 1992 to 2006. We predicted that both sexes would maintain spatially exclusive home ranges and that subadult female tigers would tend toward philopatry and males would disperse. Home ranges (95% fixed kernel estimates; mean ± SD) of resident females (n = 20 home ranges of 14 females; 390 ± 136 km²) were significantly (P = 0.003) smaller than those of males (n = 6 home ranges of 5 males; 1,385 ± 539 km²). Geometric mean overlap between adjacent females (0.11 ± 0.11 SD) did not differ from that between adjacent males (0.14 ± 0.12). All radiocollared male cubs dispersed (n = 7), but only 2 of 6 female cubs dispersed from their natal home ranges. When human-caused mortality was low, female tigers survived long enough to divide their home range with their daughters, resulting in smaller home ranges and a higher density of breeding females. All females reproduced in these smaller territories, suggesting that they maintained home ranges that were larger than needed to meet reproductive demands. However, when human-caused mortality was high, females often did not survive long enough to bequeath home ranges to daughters, and population density was apparently maintained well below carrying capacity. The impacts of poaching appear to extend beyond the direct loss of individuals, and therefore reserves must be well protected if they are to serve as source populations for adjacent, unprotected areas of tiger habitat. [ABSTRACT FROM AUTHOR]

We tested the hypothesis that sex-biased natal dispersal reduces close inbreeding in American black bears, a solitary species that exhibits nearly complete male dispersal and female philopatry. Using microsatellite DNA and spatial data from reproductively mature bears (≥ 4 years old), we examined the spatial genetic structure of two distinct populations in New Mexico from 1993 to 2000. As predicted, relatedness ( r) and the frequency of close relationships (parent–offspring or full siblings) decreased with distance among female dyads, but little change was observed among male or opposite-sex dyads. Neighbouring females were more closely related than neighbouring males. The potential for inbreeding was low. Most opposite-sex pairs that lived sufficiently close to facilitate mating were unrelated, and few were close relatives. We found no evidence that bears actively avoided inbreeding in their selection of mates from this nearby pool, as mean r and relationship frequencies did not differ between potential and actual mating pairs (determined by parentage analysis). These basic patterns were apparent in both study areas despite a nearly two-fold difference in density. However, the sex bias in dispersal was less pronounced in the lower-density area, based on proportions of bears with male and female relatives residing nearby. This result suggests that male bears may respond to reduced competition by decreasing their rate or distance of dispersal. Evidence supports the hypothesis that inbreeding avoidance is achieved by means of male-biased dispersal but also indicates that competition (for mates or resources) modifies dispersal patterns. [ABSTRACT FROM AUTHOR]

Reports on the effectiveness of using late fall hunting seasons to reduce the proportion of female black bears (Ursus americanus) in the harvest are limited, and the geographic scale over which the technique functions as intended has not been examined. During 1992-2000, we radio-equipped black bears in New Mexico, USA, obtained estimates of 175 den entry and 137 den emergence dates, and used New Mexico Department of Game and Fish harvest data (1985-2000) to test for differences in proportion of females in the harvest relative to denning chronology. Bears in northern New Mexico entered dens earlier and emerged later than bears in southern New Mexico (P ≤ 0.001). In northern New Mexico bears displayed the typical pattern of earlier entry and later emergence by reproductive females, proportion of females in the harvest varied over time as expected, and late fall seasons were effective (P ≤ 0.10). In contrast, denning chronology did not differ by sex in southern New Mexico, proportion of females in the harvest did not change over time, and late fall seasons were not effective (P ≥ 0.18). Manipulation of hunting season dates to influence female mortality can be an effective tool, however our study provides an example of an area where denning chronology did not differ by sex and late seasons were not effective. We also observed regional differences in timing of entrance and emergence, which suggest that scale of application may be key. In management jurisdictions that encompass ecologically distinct areas, cover a wide range of latitudes, or are mountainous, successful use of the technique may depend on knowledge of denning chronology at multiple locations and appropriate designation of hunting unit boundaries, season dates, and data analysis units. [ABSTRACT FROM AUTHOR]

At the request of the U.S. Fish and Wildlife Service (USFWS) and the Florida Fish and Wildlife Conservation Commission (FWC), we provide an independent assessment of the reliability of the scientific literature used to support conservation of Florida panthers (Puma concolor coryi). We independently reached similar conclusions about unreliable scientific inferences before discussing the issues with each other or with others. Although a quarter-century of research supports many published conclusions, 2 sets of unreliable inferences may compromise efforts to conserve the species The first is a set of 4 unreliable inferences that underlie the Panther Habitat Evaluation Model (PHEM), used by agencies to evaluate projects that may affect panther habitat. Specifically, the following assertions are unreliable: 1) panthers are forest obligates, 2) panthers require large (>500 ha) forest patches, 3) panthers are reluctant to cross 90-m gaps of nonforest habitat, and 4) the value of potential panther habitat declines linearly with distance to a population core in south Florida, USA. These assertions are unreliable because the analyses excluded (without mention or rationale) almost half the available data, compared used habitats to an inappropriate set of available habitats, made inferences about habitat preference without any data on available habitats, were based only on panther locations during daytime, ignored telemetry error, or suffered from other flaws. The second is a set of 2 unreliable inferences about panther demography prior to the genetic restoration effort initiated in 1995. Inferences that neonate survival was ≥0.84 and that the panther population was demographically vigorous prior to 1995 are flawed because the survival analysis ignored mortality during the first 4 months and because other inferences were based on numbers of births and deaths in samples of convenience rather than appropriate vital rates. These faulty inferences about panther demography brought unwarranted credibility to challenges of the genetic restoration program. Faulty inferences of both sets were repeated in subsequent scientific and popular articles; in several instances, previously published work was miscited. In its current (2002-2005) version, PHEM is unreliable and should not be used in decisions about panther habitat. Biologists should obtain better demographic estimates and fully analyze how the introgression program has affected these rates. [ABSTRACT FROM AUTHOR]

Eight traditional subspecies of tiger (Panthera tigris), of which three recently became extinct, are commonly recognized on the basis of geographic isolation and morphological characteristics. To investigate the species' evolutionary history and to establish objective methods for subspecies recognition, voucher specimens of blood, skin, hair, and/or skin biopsies from 134 tigers with verified geographic origins or heritage across the whole distribution range were examined for three molecular markers: (1) 4.0 kb of mitochondrial DNA (mtDNA) sequence; (2) allele variation in the nuclear major histocompatibility complex class II DRB gene; and (3) composite nuclear microsatellite genotypes based on 30 loci. Relatively low genetic variation with mtDNA, DRB, and microsatellite loci was found, but significant population subdivision was nonetheless apparent among five living subspecies. In addition, a distinct partition of the Indochinese subspecies P. t. corbetti into northern Indochinese and Malayan Peninsula populations was discovered. Population genetic structure would suggest recognition of six taxonomic units or subspecies: (1) Amur tiger P. t. altaica; (2) northern Indochinese tiger P. t. corbetti; (3) South China tiger P. t. amoyensis; (4) Malayan tiger P. t. jacksoni, named for the tiger conservationist Peter Jackson; (5) Sumatran tiger P. t. sumatrae; and (6) Bengal tiger P. t. tigris. The proposed South China tiger lineage is tentative due to limited sampling. The age of the most recent common ancestor for tiger mtDNA was estimated to be 72,000–108,000 y, relatively younger than some other Panthera species. A combination of population expansions, reduced gene flow, and genetic drift following the last genetic diminution, and the recent anthropogenic range contraction, have led to the distinct genetic partitions. These results provide an explicit basis for subspecies recognition and will lead to the improved management and conservation of these recently isolated but distinct geographic populations of tigers. Genetic analysis provides the basis for subspecies recognition among tigers, and will lead to improved conservation strategies for these endangered animals. [ABSTRACT FROM AUTHOR]

Eight traditional subspecies of tiger (Panthera tigris), of which three recently became extinct, are commonly recognized on the basis of geographic isolation and morphological characteristics. To investigate the species' evolutionary history and to establish objective methods for subspecies recognition, voucher specimens of blood, skin, hair, and/or skin biopsies from 134 tigers with verified geographic origins or heritage across the whole distribution range were examined for three molecular markers: (1) 4.0 kb of mitochondrial DNA (mtDNA) sequence; (2) allele variation in the nuclear major histocompatibility complex class II DRB gene; and (3) composite nuclear microsatellite genotypes based on 30 loci. Relatively low genetic variation with mtDNA, DRB, and microsatellite loci was found, but significant population subdivision was nonetheless apparent among five living subspecies. In addition, a distinct partition of the Indochinese subspecies P. t. corbetti into northern Indochinese and Malayan Peninsula populations was discovered. Population genetic structure would suggest recognition of six taxonomic units or subspecies: (1) Amur tiger P. t. altoko; (2) northern Indochinese tiger P. t. corbetti; (3) South China tiger P. t. amoyensis; (4) Malayan tiger P. t. jocksoni, named for the tiger conservationist Peter Jackson; (5) Sumatran tiger P. t. sumatroe; and (6) Bengal tiger P. t. tigris. The proposed South China tiger lineage is tentative due to limited sampling. The age of the most recent common ancestor for tiger mtDNA was estimated to be 72,000-108,000 y, relatively younger than some other Ponthera species. A combination of population expansions, reduced gene flow, and genetic drift following the last genetic diminution, and the recent anthropogenic range contraction, have led to the distinct genetic partitions. These results provide an explicit basis for subspecies recognition and will lead to the improved management and conservation of these recently isolated but distinct geographic populations of tigers. [ABSTRACT FROM AUTHOR]

Black bear (Ursus americanus) populations are most often managed based on sex and age composition of hunter-killed bears, but few studies have evaluated the method used to determine age. We assessed the accuracy and consistency of aging black bears in New Mexico using the cementum annuli technique by examining age estimates obtained for research-captured and hunter-killed bears. Age estimates were 80% accurate for 30 teeth obtained from 26 known-age bears. Age estimates were consistent with field estimates of age-class for 76% of 232 teeth obtained from captured bears when actual age was not known. Age estimates were consistent between paired teeth for 74% of 232 pairs taken from hunter-killed bears on the same day but for only 46% of 61 pairs taken from study bears during the same or different years. Errors or inconsistencies in age estimation increased with known or estimated age, certainty codes provided by the laboratory, and tooth breakage, but these 3 factors were correlated. Accuracy and consistency also were lower when teeth were removed during the den season. Evidence did not indicate any difference in accuracy and consistency between sexes. In comparisons of 2 blind samples obtained independently, estimated age composition of the harvest did not differ (P=0.92). Accuracy and consistency of the cementum annuli aging technique were relatively high for bears with known or estimated ages < 5 years old; therefore, the method appeared adequate for assessing age composition of annual hunter harvests in New Mexico. [ABSTRACT FROM AUTHOR]

The Greater Yellowstone Ecosystem contains the rare combination of an intact guild of native large carnivores, their prey, and differing land management policies (National Park versus National Forest; no hunting versus hunting). Concurrent field studies on large carnivores allowed us to investigate activities of humans and carnivores on Yellowstone National Park's (YNP) northern boundary. Prior to and during the backcountry big-game hunting season, we monitored movements of grizzly bears (Ursus arctos), wolves (Canis lupus), and cougars (Puma concolor) on the northern boundary of YNP. Daily aerial telemetry locations (September 1999), augmented with weekly telemetry locations (August and October 1999), were obtained for 3 grizzly bears, 7 wolves in 2 groups of 1 pack, and 3 cougars in 1 family group. Grizzly bears were more likely located inside the YNP boundary during the pre-hunt period and north of the boundary once hunting began. The cougar family tended to be found outside YNP during the pre-hunt period and moved inside YNP when hunting began. Wolves did not significantly change their movement patterns during the pre-hunt and hunting periods. Qualitative information on elk (Cervus elaphus) indicated they moved into YNP once hunting started, suggesting that cougars followed living prey or responded to hunting activity, grizzly bears focused on dead prey (e.g., gut piles, crippled elk), and wolves may have taken advantage of both. Measures of association (Jacob's Index) were positive within carnivore species but inconclusive among species. Further collaborative research and the use of new technologies such as Global Positioning System (GPS) telemetry collars will advance our ability to understand these species, the carnivore community and its interactions, and human influences on carnivores. [ABSTRACT FROM AUTHOR]

Several conceptual models describing patterns of prey selection by predators have been proposed, but such models rarely have been tested empirically, particularly with terrestrial carnivores. We examined patterns of prey selection by sympatric wolves (Canis lupus ) and cougars (Puma concolor ) to determine i) if both predators selected disadvantaged prey disproportionately from the prey population, and ii) if the specific nature and intensity of prey selection differed according to disparity in hunting behavior between predator species. We documented prey characteristics and kill site attributes of predator kills during winters 1999–2001 in Idaho, and located 120 wolf-killed and 98 cougar-killed ungulates on our study site. Elk (Cervus elephus ) were the primary prey for both predators, followed by mule deer (Odocoileus hemionus ). Both predators preyed disproportionately on elk calves and old individuals; among mule deer, wolves appeared to select for fawns, whereas cougars killed primarily adults. Nutritional status of prey, as determined by percent femur marrow fat, was consistently poorer in wolf-killed prey. We found that wolf kills occurred in habitat that was more reflective of the entire study area than cougar kills, suggesting that the coursing hunting behavior of wolves likely operated on a larger spatial scale than did the ambush hunting strategy of cougars. We concluded that the disparity in prey selection and hunting habitat between predators probably was a function of predator-specific hunting behavior and capture success, where the longer prey chases and lower capture success of wolf packs mandated a stronger selection for disadvantaged prey. For cougars, prey selection seemed to be limited primarily by prey size, which could be a function of the solitary hunting behavior of this species and the risks associated with capturing prime-aged prey. [ABSTRACT FROM AUTHOR]

Deals with a study which monitored the reproduction of female Amur tigers in the Sikhote-Alin Biosphere Zapovednik, Russia. Materials and methods; Results; Discussion.