44 results on '"Quellard N"'
Search Results
2. Ultrastructural study of rat hippocampus after chronic administration of aluminum l-glutamate: an acceleration of the aging process
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Deloncle, R, Huguet, F, Fernandez, B, Quellard, N, Babin, Ph, and Guillard, O
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- 2001
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3. Classification et prise en charge thérapeutique des gammapathies monoclonales de signification rénale
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Javaugue, V., primary, Bouteau, I., additional, Sirac, C., additional, Quellard, N., additional, Diolez, J., additional, Colombo, A., additional, Desport, E., additional, Ecotière, L., additional, Goujon, J.-M., additional, Fermand, J.-P., additional, Touchard, G., additional, Jaccard, A., additional, and Bridoux, F., additional
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- 2018
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4. Glomérulonéphrites à dépôts non organisés non Randall de chaîne légère d’immunoglobuline monoclonale : une série de sept cas
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Javaugue, V., primary, Domenger, C., additional, Bender, S., additional, Cohen, C., additional, Frémeaux-Bacchi, V., additional, Quellard, N., additional, Goujon, J.M., additional, Touchard, G., additional, Sirac, C., additional, and Bridoux, F., additional
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- 2017
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5. Pronostic de la néphropathie à cylindres myélomateux : étude rétrospective de 69 patients
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Ecotière, L., primary, Debiais, C., additional, Desport, E., additional, Goujon, J.M., additional, Gombert, J.M., additional, Quellard, N., additional, Touchard, G., additional, and Bridoux, F., additional
- Published
- 2011
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6. Le probenecid prévient la nécrose tubulaire aiguë et la fibrose interstitielle dans un modèle murin de la néphropathie aux acides aristolochiques
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Baudoux, T., primary, Pozdzik, A., additional, Arlt, V.M., additional, Hougardy, J.M., additional, de Prez, E., additional, Quellard, N., additional, Goujon, J.M., additional, and Nortier, J.L., additional
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- 2011
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7. Crystal-storing histiocytosis with renal Fanconi syndrome: pathological and molecular characteristics compared with classical myeloma-associated Fanconi syndrome
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El Hamel, C., primary, Thierry, A., additional, Trouillas, P., additional, Bridoux, F., additional, Carrion, C., additional, Quellard, N., additional, Goujon, J.-M., additional, Aldigier, J.-C., additional, Gombert, J.-M., additional, Cogne, M., additional, and Touchard, G., additional
- Published
- 2010
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8. Étude ultrastructurale des membranes épirétiniennes idiopathiques colorées au bleu trypan
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Balayre, S., primary, Boissonnot, M., additional, Fernandez, B., additional, Quellard, N., additional, Babin, P., additional, and Dighiero, P., additional
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- 2005
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9. Cadmium-containing granules in kidney tissue of the Atlantic white-sided dolphin (Lagenorhyncus acutus) off the Faroe Islands
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Gallien, I, primary, Caurant, F, additional, Bordes, M, additional, Bustamante, P, additional, Miramand, P, additional, Fernandez, B, additional, Quellard, N, additional, and Babin, P, additional
- Published
- 2001
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10. Changes in Helicobacter pylori ultrastructure and antigens during conversion from the bacillary to the coccoid form
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Benaissa, M, primary, Babin, P, additional, Quellard, N, additional, Pezennec, L, additional, Cenatiempo, Y, additional, and Fauchère, J L, additional
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- 1996
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11. Chronic administration of aluminium L-glutamate in young mature rats: effects on iron levels and lipid peroxidation in selected brain areas
- Author
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Deloncle, R., Huguet, F., Babin, P., Fernandez, B., Quellard, N., and Guillard, O.
- Published
- 1999
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12. AL Amyloidosis
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Desport Estelle, Bridoux Frank, Sirac Christophe, Delbes Sébastien, Bender Sébastien, Fernandez Béatrice, Quellard Nathalie, Lacombe Corinne, Goujon Jean-Michel, Lavergne David, Abraham Julie, Touchard Guy, Fermand Jean-Paul, and Jaccard Arnaud
- Subjects
AL amyloidosis ,Immunoglobulinic amyloidosis ,« Primary » amyloidosis ,Medicine - Abstract
Abstract Definition of the disease AL amyloidosis results from extra-cellular deposition of fibril-forming monoclonal immunoglobulin (Ig) light chains (LC) (most commonly of lambda isotype) usually secreted by a small plasma cell clone. Most patients have evidence of isolated monoclonal gammopathy or smoldering myeloma, and the occurrence of AL amyloidosis in patients with symptomatic multiple myeloma or other B-cell lymphoproliferative disorders is unusual. The key event in the development of AL amyloidosis is the change in the secondary or tertiary structure of an abnormal monoclonal LC, which results in instable conformation. This conformational change is responsible for abnormal folding of the LC, rich in β leaves, which assemble into monomers that stack together to form amyloid fibrils. Epidemiology AL amyloidosis is the most common type of systemic amyloidois in developed countries with an estimated incidence of 9 cases/million inhabitant/year. The average age of diagnosed patients is 65 years and less than 10% of patients are under 50. Clinical description The clinical presentation is protean, because of the wide number of tissues or organs that may be affected. The most common presenting symptoms are asthenia and dyspnoea, which are poorly specific and may account for delayed diagnosis. Renal manifestations are the most frequent, affecting two thirds of patients at presentation. They are characterized by heavy proteinuria, with nephrotic syndrome and impaired renal function in half of the patients. Heart involvement, which is present at diagnosis in more than 50% of patients, leading to restrictive cardiopathy, is the most serious complication and engages prognosis. Diagnostic methods The diagnosis relies on pathological examination of an involved site showing Congo red-positive amyloid deposits, with typical apple-green birefringence under polarized light, that stain positive with an anti-LC antibody by immunohistochemistry and/or immunofluorescence. Due to the systemic nature of the disease, non-invasive biopsies such as abdominal fat aspiration should be considered before taking biopsies from involved organs, in order to reduce the risk of bleeding complications. Differential diagnosis Systemic AL amyloidosis should be distinguished from other diseases related to deposition of monoclonal LC, and from other forms of systemic amyloidosis. When pathological studies have failed to identify the nature of amyloid deposits, genetic studies should be performed to diagnose hereditary amyloidosis. Management Treatment of AL amyloidosis is based on chemotherapy, aimed at controlling the underlying plasma clone that produces amyloidogenic LC. The hematological response should be carefully checked by serial measurements of serum free LC. The association of an alkylating agent with high-dose dexamethasone has proven to be effective in two thirds of patients and is considered as the current reference treatment. New agents used in the treatment of multiple myeloma are under investigation and appear to increase hematological response rates. Symptomatic measures and supportive care is necessary in patients with organ failure. Noticeably, usual treatments for cardiac failure (i.e. calcium inhibitors, β-blockers, angiotensin converting enzyme inhibitors) are inefficient or even dangerous in patients with amyloid heart disease, that should be managed using diuretics. Amiodarone and pace maker implantation should be considered in patients with rhythm or conduction abnormalities. In selected cases, heart and kidney transplantation may be associated with prolonged patient and graft survival. Prognosis Survival in AL amyloidosis depends on the spectrum of organ involvement (amyloid heart disease being the main prognosis factor), the severity of individual organs involved and haematological response to treatment.
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- 2012
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13. New clinical forms of hereditary apoA-I amyloidosis entail both glomerular and retinal amyloidosis.
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Colombat M, Aldigier JC, Rothschild PR, Javaugue V, Desport E, Frouget T, Goujon JM, Rioux-Leclercq N, Quellard N, Rerolle JP, Paraf F, Beugnet C, Tiple A, Durrbach A, Samuel D, Brézin A, Bridoux F, and Valleix S
- Subjects
- Adult, Apolipoprotein A-I genetics, Humans, Male, Retina, Amyloidosis diagnosis, Amyloidosis genetics, Amyloidosis, Familial genetics, Kidney Diseases diagnosis, Kidney Diseases genetics
- Abstract
Apolipoprotein A1 amyloidosis (ApoAI) results from specific mutations in the APOA1 gene causing abnormal accumulation of amyloid fibrils in diverse tissues. The kidney is a prominent target tissue in ApoAI amyloidosis with a remarkable selectivity for the renal medulla. Here, we investigated six French families with ApoAI Glu34Lys, p.His179Profs∗47, and a novel p.Thr185Alafs∗41 variant revealing unprecedented clinical association of a glomerular with a retinal disease. Comprehensive clinicopathological, molecular and proteomics studies of numerous affected tissues ensured the correlation between clinical manifestations, including novel unrecognized phenotypes, and apoA-I amyloid deposition. These ophthalmic manifestations stemmed from apoA-I amyloid deposition, highlighting that the retina is a previously unrecognized tissue affected by ApoAI amyloidosis. Our study provides the first molecular evidence that a significant fraction of ApoAI amyloidosis cases with no family history result from spontaneous neomutations rather than variable disease penetrance. Finally, successful hepatorenal transplantation resulted in a life- and vision-saving measure for a 32-year-old man with a hitherto unreported severe ApoAI amyloidosis caused by the very rare Glu34Lys variant. Our findings reveal new modes of occurrence and expand the clinical spectrum of ApoAI amyloidosis. The awareness of glomerular and ocular manifestations in ApoAI amyloidosis should enable earlier diagnosis and avoid misdiagnosis with other forms of renal amyloidosis. Thus, documented apoA-I amyloid deposition in the retina offers new biological information about this disease and may change organ transplantation practice to reduce retinal damage in patients with ApoAI amyloidosis., (Published by Elsevier Inc.)
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- 2020
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14. Renal toxicities associated with pembrolizumab.
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Izzedine H, Mathian A, Champiat S, Picard C, Mateus C, Routier E, Varga A, Malka D, Leary A, Michels J, Michot JM, Marabelle A, Lambotte O, Amoura Z, Soria JC, Kaaki S, Quellard N, Goujon JM, and Brocheriou I
- Abstract
Objective: Expanded clinical experience with patients treated by pembrolizumab has accumulated. However, renal toxicities associated with this anti-programmed cell death 1 agent are poorly described because kidney histology is rarely sought. As a nephrology referral centre, we aimed to describe the clinic-biological and histopathological characteristics of pembrolizumab-related nephropathy and its response to treatment., Methods: We conducted a monocentric large case series study, including all pembrolizumab-treated cancer patients presenting a renal toxicity addressed to our centre from 2015 to 2017., Results: A total of 12 patients (7 men) out of 676 pembrolizumab-treated patients (incidence 1.77%) were included (median age 69.75 years). Patients were referred for acute kidney injury ( n = 10) and/or proteinuria ( n = 2). A kidney biopsy was performed in all patients, with a median duration of use of 9 months (range 1-24 months) after the beginning of treatment. Biopsy showed that four patients had acute interstitial nephritis (AIN), whereas five had acute tubular injury (ATI) alone, one had minimal change disease (MCD) and ATI, and one had MCD alone. Pembrolizumab withdrawal coupled with corticosteroid therapy was the most effective treatment for kidney function recovery. Drug reintroduction resulted in a more severe recurrence of AIN in one patient who required maintenance of pembrolizumab. Two patients died of cancer progression with one of them developing severe renal failure requiring dialysis., Conclusion: In our series, ATI, AIN and MCD are the most frequent forms of kidney involvement under pembrolizumab therapy. Kidney dysfunction is usually isolated but can be severe. Use of corticosteroids in case of AIN improves the glomerular filtration rate.
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- 2019
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15. Cutibacterium acnes protects Candida albicans from the effect of micafungin in biofilms.
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Bernard C, Renaudeau N, Mollichella ML, Quellard N, Girardot M, and Imbert C
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- Antifungal Agents metabolism, Biofilms drug effects, Candida albicans growth & development, Flow Cytometry, Humans, Micafungin metabolism, Microbiological Techniques, Microscopy, Electron, Scanning, Propionibacterium acnes growth & development, Antifungal Agents pharmacology, Biofilms growth & development, Candida albicans drug effects, Micafungin pharmacology, Microbial Interactions, Microbial Viability drug effects, Propionibacterium acnes metabolism
- Abstract
The aim of this study was to investigate the ability of Candida albicans and Cutibacterium acnes to grow together as a polymicrobial biofilm in vitro and to examine the influence of C. acnes on C. albicans susceptibility to micafungin. Mature 72-h-old single-species biofilms of C. albicans and polymicrobial biofilms involving both C. albicans and C. acnes were formed in brain-heart infusion and were observed by scanning electronic microscopy. Moreover, 24-h-old single-species and polymicrobial biofilms were treated for 24 h with micafungin (concentrations ranging from 0.75 mg/L to 12 mg/L) and the antibiofilm activity of micafungin was evaluated on fungal cells by flow cytometry following addition of propidium iodide. The results showed that C. albicans and C. acnes formed a polymicrobial biofilm in the tested conditions and that bacterial presence did not modify fungal viability. Micafungin induced a fungal mortality rate ranging from 70-95% in C. albicans single-species biofilms and from 35-40% in C. acnes-C. albicans polymicrobial biofilms. Mortality induced by micafungin was significantly reduced (P < 0.05 for micafungin at 6 mg/L and P < 0.001 for other micafungin concentrations) in polymicrobial conditions compared with single-species biofilms. In conclusion, this study showed that C. albicans and C. acnes are able to form polymicrobial biofilms together in a synergistic way and that this organisation increases yeast resistance to micafungin., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Published
- 2018
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16. Mott cell interstitial nephritis revealing Waldenström macroglobulinaemia.
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Moktefi A, Dudreuilh C, Goujon JM, Quellard N, Zini JM, Audard V, and Rémy P
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- Aged, Humans, Male, Nephritis, Interstitial blood, Nephritis, Interstitial diagnosis, Nephritis, Interstitial pathology, Waldenstrom Macroglobulinemia blood, Waldenstrom Macroglobulinemia diagnosis, Waldenstrom Macroglobulinemia pathology
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- 2018
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17. [Classification and therapeutic management of monoclonal gammopathies of renal significance].
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Javaugue V, Bouteau I, Sirac C, Quellard N, Diolez J, Colombo A, Desport E, Ecotière L, Goujon JM, Fermand JP, Touchard G, Jaccard A, and Bridoux F
- Subjects
- Amyloidosis complications, Amyloidosis epidemiology, Amyloidosis pathology, Amyloidosis therapy, Diagnostic Techniques and Procedures, Humans, Kidney pathology, Kidney Diseases classification, Kidney Diseases etiology, Kidney Diseases therapy, Paraproteinemias classification, Paraproteinemias complications, Paraproteinemias therapy
- Abstract
Two categories of renal disorders associated with monoclonal gammopathies are to be distinguished, according to the characteristics of the underlying B-cell clone. The first group of renal diseases always occurs in the setting of high tumor mass with production of large amounts of monoclonal immunoglobulins. The main complication is the so-called myeloma cast nephropathy, which almost invariably complicates high tumor mass myeloma. The second group includes all renal disorders caused by a monoclonal immunoglobulin secreted by a nonmalignant B-cell clone, and currently referred as a "monoclonal gammopathy of renal significance (MGRS)". This term was introduced to distinguish monoclonal gammopathies that are responsible for the development of kidney damage from those that are truly benign. The spectrum of renal diseases in MGRS is wide and its classification relies on the localization of renal lesions, either glomerular or tubular, and on the pattern of ultrastructural organization of immunoglobulin deposits. Physicochemical characteristics of the pathogenic monoclonal immunoglobulin are probably involved in their propensity to deposit or precipitate in the kidney, as illustrated by the high rate of recurrence of each specific type after kidney transplantation. Early diagnosis and efficient chemotherapy targeting the causal B-cell clone are mandatory to improve renal prognosis and patient survival., (Copyright © 2017 Société Nationale Française de Médecine Interne (SNFMI). Published by Elsevier SAS. All rights reserved.)
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- 2018
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18. Decoding cold ischaemia time impact on kidney graft: the kinetics of the unfolded protein response pathways.
- Author
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Le Pape S, Pasini-Chabot O, Couturier P, Delpech PO, Volmer R, Quellard N, Ploeg R, Hauet T, and Thuillier R
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- Animals, Humans, Kidney pathology, Mice, Mice, Knockout, Swine, Cold Ischemia, Gene Expression Regulation, Kidney metabolism, Kidney Transplantation, Unfolded Protein Response
- Abstract
The relationship between cold ischaemia time (CIT) and adverse outcome is now acknowledged. However, the underlying mechanisms remain to be defined, which slows the development of adapted therapeutics and diagnostics. We explored the impact of CIT in both preclinical and in vitro models of preservation. We determined that the endoplasmic reticulum (ER) and its stress response (unfolded protein response, UPR) were regulated in close association with CIT; the eIF2α-ATF4 pathway was inhibited early (1-8 h) at the detriment of cell survival, while the ATF6 pathway was activated late (12-24 h) and associated with cell death. The IRE1α-XBP1 branch was activated at reperfusion only if CIT extended beyond 8 h, and had a dual role on cell fate - deleterious through IRE1's RNase activity and beneficial through IRE1α other roles. Finally, the pro-apoptotic factor CHOP was a common target of both ATF6 and IRE1α pathways and was associated with elongated CIT and increased cell death. Microarray analysis of human transplanted kidney confirmed that UPR markers were regulated by CIT and that CHOP was associated with adverse outcome. We show that UPR could be a critical pathway explaining the relationship between CIT and graft outcome, highlighting the potential for UPR-based therapeutics and diagnostics to improve transplantation.
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- 2018
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19. Proliferative lupus nephritis in the absence of overt systemic lupus erythematosus: A historical study of 12 adult patients.
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Touzot M, Terrier CS, Faguer S, Masson I, François H, Couzi L, Hummel A, Quellard N, Touchard G, Jourde-Chiche N, Goujon JM, and Daugas E
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- Adolescent, Adult, Biomarkers urine, Creatinine urine, Disease Progression, Female, Follow-Up Studies, Humans, Immunosuppression Therapy, Kidney pathology, Lupus Nephritis pathology, Male, Microscopy, Electron, Middle Aged, Proteinuria urine, Retrospective Studies, Treatment Outcome, Young Adult, Lupus Nephritis diagnosis, Lupus Nephritis therapy
- Abstract
Severe lupus nephritis in the absence of systemic lupus erythematosus (SLE) is a rare condition with an unclear clinical presentation and outcome.We conducted a historical observational study of 12 adult (age >18 years) patients with biopsy-proven severe lupus nephritis or lupus-like nephritis without SLE immunological markers at diagnosis or during follow-up. Excluded were patients with chronic infections with HIV or hepatitis B or C; patients with a bacterial infectious disease; and patients with pure membranous nephropathy. Electron microscopy was retrospectively performed when the material was available. End points were the proportion of patients with a complete response (urine protein to creatinine ratio <0.5 g/day and a normal or near-normal eGFR), partial response (≥50% reduction in proteinuria to subnephrotic levels and a normal or near-normal eGFR), or nonresponse at 12 months or later after the initiation of the treatment.The study included 12 patients (66% female) with a median age of 36.5 years. At diagnosis, median creatinine and proteinuria levels were 1.21 mg/dL (range 0.5-11.6) and 7.5 g/day (1.4-26.7), respectively. Six patients had nephrotic syndrome and acute kidney injury. Renal biopsy examinations revealed class III or class IV A/C lupus nephritis in all cases. Electron microscopy was performed on samples from 5 patients. The results showed mesangial and subendothelial dense deposits consistent with LN in 4 cases, and a retrospective diagnosis of pseudo-amyloid fibrillary glomerulonephritis was made in 1 patient.Patients received immunosuppressive therapy consisting of induction therapy followed by maintenance therapy, similar to treatment for severe lupus nephritis. Remission was recorded in 10 patients at 12 months after the initiation of treatment. One patient reached end-stage renal disease. After a median follow-up of 24 months, 2 patients relapsed.Lupus nephritis in the absence of overt SLE is a nosological entity requiring careful etiological investigation, including systematic electron microscopy examination of renal biopsies to rule out fibrillary glomerulonephritis. In this series, most patients presented with severe glomerulonephritis, which was highly similar to lupus nephritis at presentation and in terms of response to immunosuppressive therapy.
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- 2017
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20. DNAJB9 Is a Specific Immunohistochemical Marker for Fibrillary Glomerulonephritis.
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Nasr SH, Vrana JA, Dasari S, Bridoux F, Fidler ME, Kaaki S, Quellard N, Rinsant A, Goujon JM, Sethi S, Fervenza FC, Cornell LD, Said SM, McPhail ED, Herrera Hernandez LP, Grande JP, Hogan MC, Lieske JC, Leung N, Kurtin PJ, and Alexander MP
- Abstract
Introduction: Fibrillary glomerulonephritis (FGN) is a rare disease with unknown pathogenesis and a poor prognosis. Until now, the diagnosis of this disease has required demonstration of glomerular deposition of randomly oriented fibrils by electron microscopy that are Congo red negative and stain with antisera to Igs. We recently discovered a novel proteomic tissue biomarker for FGN, namely, DNAJB9., Methods: In this work, we developed DNAJB9 immunohistochemistry and tested its sensitivity and specificity for the diagnosis of FGN. This testing was performed on renal biopsy samples from patients with FGN (n = 84), amyloidosis (n = 21), a wide variety of non-FGN glomerular diseases (n = 98), and healthy subjects (n = 11). We also performed immunoelectron microscopy to determine whether DNAJB9 is localized to FGN fibrils., Results: Strong, homogeneous, smudgy DNAJB9 staining of glomerular deposits was seen in all but 2 cases of FGN. The 2 cases that did not stain for DNAJB9 were unique, as they had glomerular staining for IgG only (without κ or λ) on immunofluorescence. DNAJB9 staining was not observed in cases of amyloidosis, in healthy subjects, or in non-FGN glomerular diseases (with the exception of very focal staining in 1 case of smoking-related glomerulopathy), indicating 98% sensitivity and > 99% specificity. Immunoelectron microscopy showed localization of DNAJB9 to FGN fibrils but not to amyloid fibrils or immunotactoid glomerulopathy microtubules., Conclusion: DNAJB9 immunohistochemistry is sensitive and specific for FGN. Incorporation of this novel immunohistochemical biomarker into clinical practice will now allow more rapid and accurate diagnosis of this disease.
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- 2017
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21. An observational study of phagocytes and Klebsiella pneumoniae relationships: different behaviors.
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Maisonneuve E, Cateau E, Delouche M, Quellard N, and Rodier MH
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- Acanthamoeba castellanii microbiology, Cell Line, Coculture Techniques, Gentamicins pharmacology, Host-Pathogen Interactions immunology, Humans, Acanthamoeba castellanii metabolism, Host-Pathogen Interactions physiology, Klebsiella pneumoniae growth & development, Macrophages immunology, Phagocytosis physiology
- Abstract
Klebsiella pneumoniae is a bacterium that can be in relation with free living amoebae like Acanthamoeba castellanii in natural environments such as soil and water. This pathogen, which is responsible for community-acquired pneumonia and for nosocomial infections, also has interactions with host defense mechanisms like macrophages. As it has been shown that A. castellanii shares some traits with macrophages, in particular the ability to phagocyte bacteria, we have studied the uptake and the fate of the bacteria after contact with the two phagocytic cells. In our conditions, K. pneumoniae growth was increased in coculture in presence of A. castellanii or Thp-1 macrophagic cells and bacterial development was also increased by A. castellanii supernatant. In addition, we showed that the presence of the bacteria had a negative effect on the macrophages whereas it does not affect amoeba viability. Using gentamicin, which kills bacteria outside cells, we showed that only macrophages were able to internalize K. pneumoniae. This result was confirmed by electron microscopy. We have consequently reported some differences in bacterial uptake and internalization between a free living amoeba and macrophagic cells, highlighting the fact that results obtained with this amoebal model should not be extrapolated to the relationships between K. pneumoniae and macrophages., (Copyright © 2017. Published by Elsevier Masson SAS.)
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- 2017
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22. In vitro culture and phenotypic and molecular characterization of gastric stem cells from human stomach.
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Garcia M, Chomel JC, Mustapha P, Tran CT, Garnier M, Paris I, Quellard N, Godet J, Cremniter J, Bennaceur-Griscelli A, Lecron JC, Turhan AG, Burucoa C, and Bodet C
- Subjects
- Adult, Cell Differentiation, Epithelial Cells microbiology, Epithelial Cells physiology, Female, Gene Expression Profiling, Genetic Markers, Helicobacter pylori pathogenicity, Humans, Male, Microarray Analysis, Middle Aged, Real-Time Polymerase Chain Reaction, Cell Culture Techniques methods, Gastric Mucosa cytology, Stem Cells physiology
- Abstract
Background: Human gastric mucosa shows continuous self-renewal via differentiation from stem cells that remain poorly characterized., Methods: We describe an original protocol for culture of gastric stem/progenitor cells from adult human stomach. The molecular characteristics of cells were studied using TaqMan low-density array and qRT-PCR analyses using the well-characterized H1 and H9 embryonic stem cells as reference. Epithelial progenitor cells were challenged with H. pylori to characterize their inflammatory response., Results: Resident gastric stem cells expressed specific molecular markers of embryonic stem cells (SOX2, NANOG, and OCT4), as well as others specific to adult stem cells, particularly LGR5 and CD44. We show that gastric stem cells spontaneously differentiate into epithelial progenitor cells that can be challenged with H. pylori. The epithelial progenitor response to H. pylori showed a cag pathogenicity island-dependent induction of matrix metalloproteinases 1 and 3, chemokine (CXCL1, CXCL5, CXCL8, CCL20) and interleukine 33 expression., Conclusion: This study opens new outlooks for investigation of gastric stem cell biology and pathobiology as well as host-H. pylori interactions., (© 2016 John Wiley & Sons Ltd.)
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- 2017
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23. Unravelling the immunopathological mechanisms of heavy chain deposition disease with implications for clinical management.
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Bridoux F, Javaugue V, Bender S, Leroy F, Aucouturier P, Debiais-Delpech C, Goujon JM, Quellard N, Bonaud A, Clavel M, Trouillas P, Di Meo F, Gombert JM, Fermand JP, Jaccard A, Cogné M, Touchard G, and Sirac C
- Subjects
- Aged, Aged, 80 and over, Biopsy, Bortezomib therapeutic use, Drug Therapy, Combination, Female, Fluorescent Antibody Technique, France, Glomerulonephritis drug therapy, Glomerulonephritis immunology, Glomerulonephritis pathology, Heavy Chain Disease drug therapy, Heavy Chain Disease genetics, Humans, Immunoglobulin alpha-Chains analysis, Immunoglobulin gamma-Chains genetics, Immunoglobulin kappa-Chains analysis, Immunoglobulin lambda-Chains analysis, Kidney drug effects, Kidney Diseases drug therapy, Kidney Diseases pathology, Male, Middle Aged, Nephrotic Syndrome drug therapy, Nephrotic Syndrome immunology, Nephrotic Syndrome pathology, Paraproteinemias drug therapy, Paraproteinemias immunology, Polymerase Chain Reaction, Renal Insufficiency, Chronic drug therapy, Renal Insufficiency, Chronic immunology, Renal Insufficiency, Chronic pathology, Retrospective Studies, Treatment Outcome, Heavy Chain Disease immunology, Heavy Chain Disease pathology, Immunoglobulin gamma-Chains analysis, Kidney immunology, Kidney pathology, Kidney Diseases immunology
- Abstract
Randall-type heavy chain deposition disease (HCDD) is a rare disorder characterized by tissue deposition of a truncated monoclonal immunoglobulin heavy chain lacking the first constant domain. Pathophysiological mechanisms are unclear and management remains to be defined. Here we retrospectively studied 15 patients with biopsy-proven HCDD of whom 14 presented with stage 3 or higher chronic kidney disease, with nephrotic syndrome in 9. Renal lesions were characterized by nodular glomerulosclerosis, with linear peritubular and glomerular deposits of γ-heavy chain in 12 patients or α-heavy chain in 3 patients, without concurrent light chain staining. Only 2 patients had symptomatic myeloma. By serum protein electrophoresis/immunofixation, 13 patients had detectable monoclonal gammopathy. However, none of these techniques allowed detection of the nephrotoxic truncated heavy chain, which was achieved by immunoblot and/or bone marrow heavy chain sequencing in 14 of 15 patients. Serum-free kappa to lambda light chain ratio was abnormal in 11 of 11 patients so examined. Immunofluorescence studies of bone marrow plasma cells showed coexpression of the pathogenic heavy chain with light chain matching the abnormal serum-free light chain in all 3 tested patients. Heavy chain sequencing showed first constant domain deletion in 11 of 11 patients, with high isoelectric point values of the variable domain in 10 of 11 patients. All patients received chemotherapy, including bortezomib in 10 cases. Renal parameters improved in 11 patients who achieved a hematological response, as assessed by normalization of the free light chain ratio in 8 cases. Tissue deposition in HCDD relates to physicochemical peculiarities of both variable and constant heavy chain domains. Early diagnosis and treatment with bortezomib-based combinations appear important to preserve renal prognosis. Thus, monitoring of serum-free light chain is an indirect but useful method to evaluate the hematological response., (Copyright © 2016 International Society of Nephrology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2017
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24. Ultrastructural study of liver and lead tissue concentrations in young mallard ducks (Anas platyrhynchos) after ingestion of single lead shot.
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Pineau A, Fauconneau B, Plouzeau E, Fernandez B, Quellard N, Levillain P, and Guillard O
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- Animals, Diet, Eating, Environmental Pollutants metabolism, Female, Lead metabolism, Liver ultrastructure, Male, Microscopy, Electron, Transmission veterinary, Spectrophotometry, Atomic veterinary, Tissue Distribution, Ducks metabolism, Environmental Pollutants toxicity, Lead toxicity, Liver drug effects
- Abstract
Lead (Pb) represents a serious threat to wildlife and ecosystems. The aim of this study was to examine the subcellular effects of dietary Pb pellet ingestion on mallard (Anas platyrhynchos) livers. After ingestion of a single Pb shot (LS4 size class: 0.177 ± 0.03 g) in 41 mallard ducks (22 males and 19 females) versus 10 controls (5 males and 5 females), all 7-week old, a morphologic study was conducted by TEM (transmission electron microscopy) of liver at the subcellular level. The results in treated mallards showed at a magnification of 2500 X that hepatic parenchyma was altered as evidenced by intralysosomal electron-dense deposits, which are compatible with Pb deposits. Further, at a higher magnification (15,000 X) in both genders, deterioration of mitochondria was observed in which the crests and, to a lesser extent, outer membrane were lysed. While the rough endoplasmic reticulum was fragmented, intracytoplasmic electron-dense material compatible with Pb deposits was maximally visible, thereby underscoring the deeply destructive effect of this metal on the subcellular architecture of the liver. In addition, applying an optimized and validated method in a clean room using electrothermal atomic absorption spectrophotometer (ETAAS) with Zeeman background correction, the objective was to improve and refine certain indispensable measurements pertaining to Pb impregnation in tissues other than liver such as kidneys, bones, and feathers of mallards. Data demonstrated show that compared with controls, Pb accumulation increases significantly, not only in the liver (3-fold), but also in the bones and the feathers (14-fold). No significant difference was noted between males and females. Bearing in mind the marked subcellular toxicity attributed to Pb, this study reinforces present-day arguments advocating limitation of game consumption.
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- 2017
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25. Blocking TGF-β Signaling Pathway Preserves Mitochondrial Proteostasis and Reduces Early Activation of PDGFRβ+ Pericytes in Aristolochic Acid Induced Acute Kidney Injury in Wistar Male Rats.
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Pozdzik AA, Giordano L, Li G, Antoine MH, Quellard N, Godet J, De Prez E, Husson C, Declèves AE, Arlt VM, Goujon JM, Brochériou-Spelle I, Ledbetter SR, Caron N, and Nortier JL
- Subjects
- Acute Kidney Injury chemically induced, Acute Kidney Injury prevention & control, Animals, Antibodies, Neutralizing immunology, Antibodies, Neutralizing pharmacology, Aristolochic Acids, Blotting, Western, Cell Line, Cells, Cultured, Endothelial Cells drug effects, Endothelial Cells metabolism, Homeostasis drug effects, Humans, Kidney Tubules, Proximal drug effects, Kidney Tubules, Proximal metabolism, Male, Models, Biological, Myofibroblasts drug effects, Myofibroblasts metabolism, Pericytes drug effects, Rats, Wistar, Signal Transduction drug effects, Smad Proteins metabolism, Time Factors, Transforming Growth Factor beta immunology, Acute Kidney Injury metabolism, Mitochondrial Proteins metabolism, Pericytes metabolism, Receptor, Platelet-Derived Growth Factor beta metabolism, Transforming Growth Factor beta metabolism
- Abstract
Background: The platelet-derived growth factor receptor β (PDGFRβ)+ perivascular cell activation becomes increasingly recognized as a main source of scar-associated kidney myofibroblasts and recently emerged as a new cellular therapeutic target., Aims: In this regard, we first confirmed the presence of PDGFRβ+ perivascular cells in a human case of end-stage aristolochic acid nephropathy (AAN) and thereafter we focused on the early fibrosis events of transforming growth factor β (TGFβ) inhibition in a rat model of AAN., Materials and Methods: Neutralizing anti-TGFβ antibody (1D11) and its control isotype (13C4) were administered (5 mg/kg, i.p.) at Days -1, 0, 2 and 4; AA (15 mg/kg, sc) was injected daily., Results: At Day 5, 1D11 significantly suppressed p-Smad2/3 signaling pathway improving renal function impairment, reduced the score of acute tubular necrosis, peritubular capillaritis, interstitial inflammation and neoangiogenesis. 1D11 markedly decreased interstitial edema, disruption of tubular basement membrane loss of brush border, cytoplasmic edema and organelle ultrastructure alterations (mitochondrial disruption and endoplasmic reticulum edema) in proximal tubular epithelial cells. Moreover, 1D11 significantly inhibited p-PERK activation and attenuated dysregulation of unfolded protein response (UPR) pathways, endoplasmic reticulum and mitochondrial proteostasis in vivo and in vitro., Conclusions: The early inhibition of p-Smad2/3 signaling pathway improved acute renal function impairment, partially prevented epithelial-endothelial axis activation by maintaining PTEC proteostasis and reduced early PDGFRβ+ pericytes-derived myofibroblasts accumulation.
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- 2016
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26. Prognostic value of kidney biopsy in myeloma cast nephropathy: a retrospective study of 70 patients.
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Ecotière L, Thierry A, Debiais-Delpech C, Chevret S, Javaugue V, Desport E, Belmouaz S, Quellard N, Kaaki S, Michel Goujon J, Fermand JP, Touchard G, and Bridoux F
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- 2016
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27. Kidney diseases associated with monoclonal immunoglobulin M-secreting B-cell lymphoproliferative disorders: a case series of 35 patients.
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Chauvet S, Bridoux F, Ecotière L, Javaugue V, Sirac C, Arnulf B, Thierry A, Quellard N, Milin S, Bender S, Goujon JM, Jaccard A, Fermand JP, and Touchard G
- Subjects
- Acute Kidney Injury etiology, Acute Kidney Injury immunology, Acute Kidney Injury pathology, Adult, Aged, Aged, 80 and over, Amyloid immunology, Amyloidosis etiology, Amyloidosis immunology, Amyloidosis pathology, Antibodies, Monoclonal immunology, Cohort Studies, Female, Glomerulonephritis, Membranoproliferative etiology, Glomerulonephritis, Membranoproliferative immunology, Glomerulonephritis, Membranoproliferative pathology, Humans, Kidney Diseases immunology, Kidney Diseases pathology, Kidney Neoplasms immunology, Kidney Neoplasms pathology, Lymphoma, B-Cell immunology, Lymphoma, B-Cell pathology, Lymphoproliferative Disorders complications, Lymphoproliferative Disorders immunology, Male, Middle Aged, Nephritis, Interstitial etiology, Nephritis, Interstitial immunology, Nephritis, Interstitial pathology, Nephrotic Syndrome etiology, Nephrotic Syndrome immunology, Nephrotic Syndrome pathology, Paraproteinemias immunology, Paraproteinemias pathology, Retrospective Studies, Waldenstrom Macroglobulinemia immunology, Waldenstrom Macroglobulinemia pathology, B-Lymphocytes immunology, Immunoglobulin M immunology, Kidney Diseases etiology, Kidney Neoplasms complications, Lymphoma, B-Cell complications, Paraproteinemias complications, Waldenstrom Macroglobulinemia complications
- Abstract
Background: Kidney diseases associated with immunoglobulin M (IgM) monoclonal gammopathy are poorly described, with few data for patient outcomes and renal response., Study Design: Case series., Setting & Participants: 35 patients from 8 French departments of nephrology were retrospectively studied. Inclusion criteria were: (1) detectable serum monoclonal IgM, (2) estimated glomerular filtration rate (eGFR) < 60mL/min/1.73m(2) and/or proteinuria with protein excretion > 0.5g/d and/or microscopic hematuria, and (3) kidney biopsy showing monoclonal immunoglobulin deposits and/or lymphomatous B-cell renal infiltration. All patients received chemotherapy, including rituximab-based regimens in 8 cases., Predictors: Patients were classified into 3 groups according to renal pathology: glomerular AL amyloidosis (group 1; n=11), nonamyloid glomerulopathies (group 2; n=15, including 9 patients with membranoproliferative glomerulonephritis), and tubulointerstitial nephropathies (group 3; n=9, including cast nephropathy in 5, light-chain Fanconi syndrome in 3, and isolated tumor infiltration in 1)., Outcomes: Posttreatment hematologic response (≥50% reduction in serum monoclonal IgM and/or free light chain level) and renal response (≥50% reduction in 24-hour proteinuria or eGFR≥30mL/min/1.73m(2) in patients with glomerular and tubulointerstitial disorders, respectively)., Results: Nephrotic syndrome was observed in 11 and 6 patients in groups 1 and 2, respectively. Patients in group 3 presented with acute kidney injury (n=7) and/or proximal tubular dysfunction (n=3). Waldenström macroglobulinemia was present in 26 patients (8, 12, and 6 in groups 1, 2, and 3, respectively). Significant lymphomatous interstitial infiltration was observed in 18 patients (4, 9, and 5 patients, respectively). Only 9 of 29 evaluable patients had systemic signs of symptomatic hematologic disease (2, 5, and 2, respectively). In groups 1, 2, and 3, respectively, hematologic response was achieved after first-line treatment in 3 of 9, 9 of 10, and 5 of 6 evaluable patients, while renal response occurred in 5 of 10, 9 of 15, and 5 of 8 evaluable patients., Limitations: Retrospective study; insufficient population to establish the impact of chemotherapy., Conclusions: IgM monoclonal gammopathy is associated with a wide spectrum of renal manifestations, with an under-recognized frequency of tubulointerstitial disorders., (Copyright © 2015 National Kidney Foundation, Inc. Published by Elsevier Inc. All rights reserved.)
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- 2015
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28. Activity of Six Essential Oils Extracted from Tunisian Plants against Legionella pneumophila.
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Chaftar N, Girardot M, Quellard N, Labanowski J, Ghrairi T, Hani K, Frère J, and Imbert C
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- Anti-Bacterial Agents chemistry, Artemisia chemistry, Citrus sinensis chemistry, Dose-Response Relationship, Drug, Juniperus chemistry, Microbial Sensitivity Tests, Oils, Volatile chemistry, Plant Oils chemistry, Rosmarinus chemistry, Ruta chemistry, Structure-Activity Relationship, Thymus Plant chemistry, Tunisia, Anti-Bacterial Agents isolation & purification, Anti-Bacterial Agents pharmacology, Legionella pneumophila drug effects, Oils, Volatile isolation & purification, Oils, Volatile pharmacology, Plant Oils isolation & purification, Plant Oils pharmacology
- Abstract
The aim of this study was to investigate the composition of six essential oils extracted from Tunisian plants, i.e., Artemisia herba-alba Asso, Citrus sinensis (L.) Osbeck, Juniperus phoenicea L., Rosmarinus officinalis L., Ruta graveolens L., and Thymus vulgaris L., and to evaluate their activity against Legionella pneumophila (microdilution assays). Eight Legionella pneumophila strains were studied, including the two well-known serogroup 1 Lens and Paris strains as controls and six environmental strains isolated from Tunisian spas belonging to serogroups 1, 4, 5, 6, and 8. The essential oils were generally active against L. pneumophila. The activities of the A. herba-alba, C. sinensis, and R. officinalis essential oils were strain-dependent, whereas those of the J. phoenicea and T. vulgaris oils, showing the highest anti-Legionella activities, with minimum inhibitory concentrations (MICs) lower than 0.03 and lower than or equal to 0.07 mg/ml, respectively, were independent of the strains' serogroup. Moreover, the microorganisms treated with T. vulgaris essential oil were shorter, swollen, and less electron-dense compared to the untreated controls. Isoborneol (20.91%), (1S)-α-pinene (18.30%) β-phellandrene (8.08%), α-campholenal (7.91%), and α-phellandrene (7.58%) were the major components isolated from the J. phoenicea oil, while carvacrol (88.50%) was the main compound of the T. vulgaris oil, followed by p-cymene (7.86%). This study highlighted the potential interest of some essential oils extracted from Tunisian plants as biocides to prevent the Legionella risk., (Copyright © 2015 Verlag Helvetica Chimica Acta AG, Zürich.)
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- 2015
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29. A mouse model recapitulating human monoclonal heavy chain deposition disease evidences the relevance of proteasome inhibitor therapy.
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Bonaud A, Bender S, Touchard G, Lacombe C, Srour N, Delpy L, Oblet C, Druilhe A, Quellard N, Javaugue V, Cogné M, Bridoux F, and Sirac C
- Subjects
- Amino Acid Sequence, Animals, Bortezomib, Disease Models, Animal, Gene Expression, Genetic Loci, Heavy Chain Disease genetics, Heavy Chain Disease immunology, Heavy Chain Disease pathology, Humans, Immunoglobulin Heavy Chains genetics, Immunoglobulin Heavy Chains immunology, Kidney Diseases genetics, Kidney Diseases immunology, Kidney Diseases pathology, Kidney Glomerulus drug effects, Kidney Glomerulus immunology, Kidney Glomerulus metabolism, Kidney Glomerulus pathology, Mice, Mice, Transgenic, Molecular Sequence Data, Plasma Cells drug effects, Plasma Cells immunology, Plasma Cells metabolism, Plasma Cells pathology, Proteasome Endopeptidase Complex drug effects, Proteasome Endopeptidase Complex metabolism, Protein Aggregation, Pathological genetics, Protein Aggregation, Pathological immunology, Protein Aggregation, Pathological pathology, Protein Structure, Tertiary, Sequence Deletion, Unfolded Protein Response drug effects, Unfolded Protein Response genetics, Unfolded Protein Response immunology, Antineoplastic Agents pharmacology, Boronic Acids pharmacology, Heavy Chain Disease drug therapy, Immunoglobulin Heavy Chains chemistry, Kidney Diseases drug therapy, Proteasome Inhibitors pharmacology, Protein Aggregation, Pathological drug therapy, Pyrazines pharmacology
- Abstract
Randall-type heavy chain deposition disease (HCDD) is a rare disorder characterized by glomerular and peritubular amorphous deposits of a truncated monoclonal immunoglobulin heavy chain (HC) bearing a deletion of the first constant domain (CH1). We created a transgenic mouse model of HCDD using targeted insertion in the immunoglobulin κ locus of a human HC extracted from a HCDD patient. Our strategy allows the efficient expression of the human HC in mouse B and plasma cells, and conditional deletion of the CH1 domain reproduces the major event underlying HCDD. We show that the deletion of the CH1 domain dramatically reduced serum HC levels. Strikingly, even with very low serum level of truncated monoclonal HC, histologic studies revealed typical Randall-type renal lesions that were absent in mice expressing the complete human HC. Bortezomib-based treatment resulted in a strong decrease of renal deposits. We further demonstrated that this efficient response to proteasome inhibitors mostly relies on the presence of the isolated truncated HC that sensitizes plasma cells to bortezomib through an elevated unfolded protein response (UPR). This new transgenic model of HCDD efficiently recapitulates the pathophysiologic features of the disease and demonstrates that the renal damage in HCDD relies on the production of an isolated truncated HC, which, in the absence of a LC partner, displays a high propensity to aggregate even at very low concentration. It also brings new insights into the efficacy of proteasome inhibitor-based therapy in this pathology., (© 2015 by The American Society of Hematology.)
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- 2015
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30. Morphological Study of the Encystment and Excystment of Vermamoeba vermiformis Revealed Original Traits.
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Fouque E, Yefimova M, Trouilhé MC, Quellard N, Fernandez B, Rodier MH, Thomas V, Humeau P, and Héchard Y
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- Microscopy, Video, Time Factors, Lobosea cytology, Lobosea physiology, Spores, Protozoan cytology, Spores, Protozoan physiology
- Abstract
Free-living amoebae are ubiquitous protozoa commonly found in water. Among them, Acanthamoeba and Vermamoeba (formerly Hartmannella) are the most represented genera. In case of stress, such as nutrient deprivation or osmotic stress, these amoebae initiate a differentiation process, named encystment. It leads to the cyst form, which is a resistant form enabling amoebae to survive in harsh conditions and resist disinfection treatments. Encystment has been thoroughly described in Acanthamoeba but poorly in Vermamoeba. Our study was aimed to follow the encystment/excystment processes by microscopic observations. We show that encystment is quite rapid, as mature cysts were obtained in 9 h, and that cyst wall is composed of two layers. A video shows that a locomotive form is likely involved in clustering cysts together during encystment. As for Acanthamoeba, autophagy is likely active during this process. Specific vesicles, possibly involved in ribophagy, were observed within the cytoplasm. Remarkably, mitochondria rearranged around the nucleus within the cyst, suggesting high needs in energy. Unlike Acanthamoeba and Naegleria, no ostioles were observed in the cyst wall suggesting that excystment is original. During excystment, large vesicles, likely filled with hydrolases, were found in close proximity to cyst wall and digest it. Trophozoite moves inside its cyst wall before exiting during excystment. In conclusion, Vermamoeba encystment/excystment displays original trends as compare to Acanthamoeba., (© 2014 The Author(s) Journal of Eukaryotic Microbiology © 2014 International Society of Protistologists.)
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- 2015
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31. Stenotrophomonas maltophilia and Vermamoeba vermiformis relationships: bacterial multiplication and protection in amoebal-derived structures.
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Cateau E, Maisonneuve E, Peguilhan S, Quellard N, Hechard Y, and Rodier MH
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- Amoeba physiology, Animals, Stenotrophomonas maltophilia growth & development, Water Microbiology, Amoeba microbiology, Stenotrophomonas maltophilia physiology
- Abstract
Stenotrophomonas maltophilia, a bacteria involved in healthcare-associated infections, can be found in hospital water systems. Other microorganisms, such as Free Living amoebae (FLA), are also at times recovered in the same environment. Amongst these protozoa, many authors have reported the presence of Vermamoeba vermiformis. We show here that this amoeba enhances S. maltophilia growth and harbors the bacteria in amoebal-derived structures after 28 days in harsh conditions. These results highlight the fact that particular attention should be paid to the presence of FLA in hospital water systems, because of their potential implication in survival and growth of pathogenic bacterial species., (Copyright © 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
- Published
- 2014
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32. Impairment of autophagy in the central nervous system during lipopolysaccharide-induced inflammatory stress in mice.
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François A, Terro F, Quellard N, Fernandez B, Chassaing D, Janet T, Rioux Bilan A, Paccalin M, and Page G
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- Animals, Biomarkers metabolism, Cerebral Cortex metabolism, Cerebral Cortex pathology, Cerebral Cortex ultrastructure, Cytokines metabolism, Female, Hippocampus metabolism, Hippocampus pathology, Hippocampus ultrastructure, Inflammation metabolism, Inflammation Mediators metabolism, Lipopolysaccharides, Male, Mice, Signal Transduction, TOR Serine-Threonine Kinases metabolism, Autophagy, Central Nervous System pathology, Inflammation pathology, Stress, Physiological
- Abstract
Background: Current evidence suggests a central role for autophagy in many neurodegenerative diseases including Alzheimer's disease, Huntington's disease, Parkinson's disease and amyotrophic lateral sclerosis. Furthermore, it is well admitted that inflammation contributes to the progression of these diseases. Interestingly, crosstalks between autophagy and inflammation have been reported in vitro and at the peripheral level such as in Crohn's disease. However, the impact of systemic inflammation on autophagic components in the brain remains to be documented. Therefore, this study monitored autophagy markers after acute and chronic lipopolysaccharide (LPS)-induced inflammatory stress in mice., Results: We showed that acute inflammation, 24 h post-intraperitoneal 10 mg/kg LPS, substantially increased cytokine production (Interleukin(IL)-1β, Tumor necrosis factor (TNF)-α and IL-6), decreased the levels of autophagy markers (Beclin-1, p62 and LC3 II) and reduced p70S6K activation in cortex and hippocampus. In hippocampus, IL-1β levels and LC3 II expression were positively and highly correlated and a negative correlation was noted between TNF-α levels and p70S6K activation. Chronic inflammation by injection of 0.5 mg/kg LPS every three days during three months led to a moderate IL-1β production and decreased TNF-α levels. Interestingly, Beclin-1 and LC3 II levels decreased while those of p62 increased. Cortical IL-1β levels positively correlated with Beclin-1 and LC3 II and on the contrary inversely correlated with p62., Conclusion: The present study is the first showing links between IL-1β-mediated inflammation and autophagy in the brain. It could open to new therapeutic strategies in brain diseases where regulation impairment of inflammation and autophagy progress with the severity of diseases.
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- 2014
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33. Longitudinal follow-up of autophagy and inflammation in brain of APPswePS1dE9 transgenic mice.
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François A, Rioux Bilan A, Quellard N, Fernandez B, Janet T, Chassaing D, Paccalin M, Terro F, and Page G
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- Amyloid beta-Protein Precursor genetics, Animals, Apoptosis Regulatory Proteins metabolism, Beclin-1, Brain metabolism, Brain ultrastructure, Disease Models, Animal, Female, Humans, Longitudinal Studies, Male, Mice, Mice, Transgenic, Microtubule-Associated Proteins genetics, Microtubule-Associated Proteins metabolism, Mutation genetics, Presenilin-1 genetics, Receptors, Immunologic metabolism, Signal Transduction genetics, TOR Serine-Threonine Kinases metabolism, Autophagy genetics, Brain pathology, Cytokines metabolism, Encephalitis genetics, Encephalitis pathology, Encephalitis physiopathology, Gene Expression Regulation genetics
- Abstract
Background: In recent years, studies have sought to understand the mechanisms involved in the alteration of autophagic flux in Alzheimer's disease (AD). Alongside the recent description of the impairment of lysosomal acidification, we wanted to study the relationships between inflammation and autophagy, two physiological components deregulated in AD. Therefore, a longitudinal study was performed in APPswePS1dE9 transgenic mice at three, six and twelve months of age., Methods: Autophagic markers (Beclin-1, p62 and LC3) and the activation of mammalian Target of Rapamycin (mTOR) signaling pathway were quantified by western blot. Cytokine levels (IL-1β, TNF-α and IL-6) were measured by ELISA. Transmission electron microscopy was performed to detect autophagic vacuoles. Mann-Whitney tests were used to compare wild-type (WT) versus APPswePS1dE9 mice. Longitudinal changes in parameters were analyzed with a Kruskal-Wallis test followed by a post-hoc Dunn's test. Correlation between two parameters was assessed using a Spearman test., Results: Compared to 12-month old WT mice, 12-month old APPswePS1dE9 mice had higher levels of IL-1β and TNF-α, a greater inhibition of the mTOR signaling pathway and lower levels of Beclin-1 expression both in cortex and hippocampus. Regarding the relationship of the various parameters in 12-month old APPswePS1dE9 mice, Beclin-1 rates were positively correlated with IL-1β and TNF-α levels. And, on the contrary, TNF-α levels were inversely correlated with the levels of mTOR activation. Altogether, these results suggest that inflammation could induce autophagy in APPswePS1dE9 mice. However, these transgenic mice displayed a large accumulation of autophagic vesicles within dystrophic neurons in cortex and hippocampus, indicating a terminal failure in the autophagic process., Conclusions: This first demonstration of relationships between inflammation and autophagy in in vivo models of AD should be taken into account in new therapeutic strategies to prevent inflammation and/or stimulate autophagy in advanced neurodegenerative process such as AD.
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- 2014
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34. Kidney protection by hypothermic total liquid ventilation after cardiac arrest in rabbits.
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Tissier R, Giraud S, Quellard N, Fernandez B, Lidouren F, Darbera L, Kohlhauer M, Pons S, Chenoune M, Bruneval P, Goujon JM, Ghaleh B, Berdeaux A, and Hauet T
- Subjects
- Animals, Disease Models, Animal, Kidney physiopathology, Kidney Function Tests, Rabbits, Treatment Outcome, Heart Arrest complications, Hypothermia, Induced methods, Kidney Diseases complications, Kidney Diseases prevention & control, Liquid Ventilation methods
- Abstract
Background: Total liquid ventilation (TLV) with perfluorocarbons has been shown to induce rapid protective cooling in animal models of myocardial ischemia and cardiac arrest, with improved neurological and cardiovascular outcomes after resuscitation. In this study, the authors hypothesized that hypothermic TLV can also limit kidney injury after cardiac arrest., Methods: Anesthetized rabbits were submitted to 15 min of untreated ventricular fibrillation. After resuscitation, three groups of eight rabbits each were studied such as (1) life support plus hypothermia (32°-33 °C) induced by cold TLV (TLV group), (2) life support without hypothermia (control group), and (3) Sham group (no cardiac arrest). Life support was continued for 6 h before euthanasia and kidney removal., Results: Time to target esophageal temperature was less than 5 min in the TLV group. Hypothermia was accompanied by preserved renal function in the TLV group as compared with control group regarding numerous markers including creatinine blood levels (12 ± 1 vs. 16 ± 2 mg/l, respectively; mean ± SEM), urinary N-acetyl-β-(D)-glucosaminidase (1.70 ± 0.11 vs. 3.07 ± 0.10 U/mol of creatinine), γ-glutamyltransferase (8.36 ± 0.29 vs. 12.96 ± 0.44 U/mol of creatinine), or β2-microglobulin (0.44 ± 0.01 vs. 1.12 ± 0.04 U/mol of creatinine). Kidney lesions evaluated by electron microscopy and conventional histology were also attenuated in TLV versus control groups. The renal-protective effect of TLV was not related to differences in delayed inflammatory or immune renal responses because transcriptions of, for example, interferon-γ, tumor necrosis factor-α, interleukin-1β, monocyte chemoattractant protein-1, toll-like receptor-2, toll-like receptor-4, and vascular endothelial growth factor were similarly altered in TLV and control versus Sham., Conclusion: Ultrafast cooling with TLV is renal protective after cardiac arrest and resuscitation, which could increase kidney availability for organ donation.
- Published
- 2014
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35. Leptospira Interrogans induces fibrosis in the mouse kidney through Inos-dependent, TLR- and NLR-independent signaling pathways.
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Fanton d'Andon M, Quellard N, Fernandez B, Ratet G, Lacroix-Lamandé S, Vandewalle A, Boneca IG, Goujon JM, and Werts C
- Subjects
- Animals, Carrier State immunology, Carrier State microbiology, Female, Fibrosis immunology, Fibrosis microbiology, Immunohistochemistry, Kidney immunology, Kidney microbiology, Kidney pathology, Kidney Diseases immunology, Kidney Diseases microbiology, Leptospirosis immunology, Leptospirosis microbiology, Mice, Mice, Inbred C57BL, Mice, Transgenic, Real-Time Polymerase Chain Reaction, Receptors, G-Protein-Coupled metabolism, Toll-Like Receptors metabolism, Fibrosis pathology, Kidney Diseases pathology, Leptospira interrogans immunology, Leptospirosis pathology, Nitric Oxide Synthase Type II metabolism, Signal Transduction
- Abstract
Background: Leptospira (L.) interrogans are bacteria responsible for a worldwide reemerging zoonosis. Rodents carry L. interrogans asymptomatically in their kidneys and excrete bacteria in the urine, contaminating the environment. Humans get infected through skin contact and develop a mild or severe leptospirosis that may lead to renal failure and fibrosis. L. interrogans provoke an interstitial nephritis, but the induction of fibrosis caused by L. interrogans has not been studied in murine models. Innate immune receptors from the TLR and NLR families have recently been shown to play a role in the development and progression of tissue fibrosis in the lung, liver and kidneys under different pathophysiological situations. We recently showed that TLR2, TLR4, and NLRP3 receptors were crucial in the defense against leptospirosis. Moreover, infection of a human cell line with L. interrogans was shown to induce TLR2-dependent production of fibronectin, a component of the extracellular matrix. Therefore, we thought to assess the presence of renal fibrosis in L. interrogans infected mice and to analyze the contribution of some innate immune pathways in this process., Methodology/principal Findings: Here, we characterized by immunohistochemical studies and quantitative real-time PCR, a model of Leptospira-infected C57BL/6J mice, with chronic carriage of L. interrogans inducing mild renal fibrosis. Using various strains of transgenic mice, we determined that the renal infiltrates of T cells and, unexpectedly, TLR and NLR receptors, are not required to generate Leptospira-induced renal fibrosis. We also show that the iNOS enzyme, known to play a role in Leptospira-induced interstitial nephritis, also plays a role in the induction of renal fibrosis., Conclusion/significance: To our knowledge, this work provides the first experimental murine model of sustained renal fibrosis induced by a chronic bacterial infection that may be peculiar, since it does not rely on TLR or NLR receptors. This model may prove useful to test future therapeutic strategies to combat Leptospira-induced renal lesions.
- Published
- 2014
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36. Probenecid prevents acute tubular necrosis in a mouse model of aristolochic acid nephropathy.
- Author
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Baudoux TE, Pozdzik AA, Arlt VM, De Prez EG, Antoine MH, Quellard N, Goujon JM, and Nortier JL
- Subjects
- Animals, Atrophy, Biomarkers blood, Cell Proliferation drug effects, Cell Survival drug effects, Creatinine blood, Cytoprotection, DNA Adducts metabolism, Disease Models, Animal, Fibrosis, Kidney Tubular Necrosis, Acute blood, Kidney Tubular Necrosis, Acute chemically induced, Kidney Tubular Necrosis, Acute pathology, Kidney Tubules metabolism, Kidney Tubules ultrastructure, Male, Mice, Mice, Inbred C57BL, Nephritis, Interstitial blood, Nephritis, Interstitial chemically induced, Nephritis, Interstitial pathology, Organic Anion Transporters antagonists & inhibitors, Organic Anion Transporters metabolism, Proliferating Cell Nuclear Antigen metabolism, Time Factors, Aristolochic Acids, Kidney Tubular Necrosis, Acute prevention & control, Kidney Tubules drug effects, Nephritis, Interstitial prevention & control, Probenecid pharmacology, Protective Agents pharmacology
- Abstract
Experimental aristolochic acid nephropathy is characterized by early tubulointerstitial injury followed by fibrosis, reproducing chronic lesions seen in humans. In vitro, probenecid inhibits aristolochic acid entry through organic anion transporters, reduces specific aristolochic acid-DNA adduct formation, and preserves cellular viability. To test this in vivo, we used a mouse model of aristolochic acid nephropathy displaying severe tubulointerstitial injuries consisting of proximal tubular epithelial cell necrosis associated to transient acute kidney injury followed by mononuclear cell infiltration, tubular atrophy, and interstitial fibrosis. Treatment with probenecid prevented increased plasma creatinine and tubulointerstitial injuries, and reduced both the extent and the severity of ultrastructural lesions induced by aristolochic acid, such as the loss of brush border, mitochondrial edema, and the disappearance of mitochondrial crests. Further, the number of proliferating cell nuclear antigen-positive cells and total aristolochic acid-DNA adducts were significantly reduced in mice receiving aristolochic acid plus probenecid compared with mice treated with aristolochic acid alone. Thus, we establish the nephroprotective effect of probenecid, an inhibitor of organic acid transporters, in vivo toward acute proximal tubular epithelial cell toxicity in a mouse model of aristolochic acid nephropathy.
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- 2012
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37. Fanconi syndrome and chronic kidney disease in paroxysmal nocturnal hemoglobinuria: effect of eculizumab therapy.
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Moumas E, Bridoux F, Leroy F, Belmouaz S, Randriamalala E, Desport E, Dreyfus B, Delbès S, Quellard N, and Touchard G
- Subjects
- Aged, Chronic Disease, Female, Hemoglobinuria, Paroxysmal drug therapy, Humans, Male, Middle Aged, Antibodies, Monoclonal, Humanized therapeutic use, Fanconi Syndrome etiology, Hemoglobinuria, Paroxysmal complications, Kidney Diseases etiology
- Abstract
The association of Fanconi syndrome (FS) and chronic kidney disease (CKD) has been rarely described during the course of paroxysmal nocturnal hemoglobinuria (PNH). We report 2 patients with PNH and CKD associated with proximal tubule dysfunction, which manifested as full-blown FS in one case. In both patients, abnormal iron load within the kidneys was demonstrated by magnetic resonance imaging, which correlated with diffuse and numerous hemosiderin inclusions within proximal tubular cells. After 12 months, eculizumab treatment resulted in significant decrease in the kidney iron load in both cases. Glomerular filtration rate improved in one case and was stabilized in the other, in whom pretreatment kidney biopsy had shown severe extensive interstitial fibrosis. However, symptoms of proximal tubular dysfunction persisted in both patients. These data suggest that hemosiderin deposition in proximal tubules is probably an important mechanism involved in the development of FS, an under recognized and early manifestation of CKD in PNH. Prolonged treatment with eculizumab may improve long-term renal function in PNH patients with CKD.
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- 2012
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38. Glomerulonephritis with isolated C3 deposits and monoclonal gammopathy: a fortuitous association?
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Bridoux F, Desport E, Frémeaux-Bacchi V, Chong CF, Gombert JM, Lacombe C, Quellard N, and Touchard G
- Subjects
- Adult, Aged, Complement Pathway, Alternative, Female, Follow-Up Studies, Glomerulonephritis, Membranoproliferative immunology, Glomerulonephritis, Membranoproliferative pathology, Glomerulonephritis, Membranoproliferative therapy, Humans, Male, Middle Aged, Retrospective Studies, Complement C3 analysis, Glomerulonephritis, Membranoproliferative etiology, Paraproteinemias complications
- Abstract
Background and Objectives: Glomerular deposition of monoclonal Ig has been exceptionally described as the cause of membranoproliferative glomerulonephritis, through activation of the complement alternative pathway (CAP)., Design, Setting, Participants, & Measurements: We retrospectively studied six adults with monoclonal gammopathy and glomerulonephritis (GN) characterized by isolated C3 deposits., Results: All patients presented with hematuria, associated with chronic renal failure and proteinuria in five patients, three of whom had nephrotic syndrome. Five patients had monoclonal gammopathy of undetermined significance and one had smoldering myeloma. The serum monoclonal IgG (κ four of six, λ two of six) was associated with light chain (LC) proteinuria in five patients. Four patients had low serum C3 and/or factor B levels. C4, factor H (CFH), and I protein levels were normal in five of five patients; none had detectable C3NeF. IgG anti-CFH activity was positive in one case. No mutations in CFH, CFI, and MCP genes were identified in four of four patients. Deposits were intramembranous, subepithelial, and mesangial by electron microscopy, and stained positive for C3 (six of six), properdin, and CFH (two of two) but negative for Ig LC and heavy chains, C4, and C1q (6/6) by immunofluorescence. Five patients progressed to end-stage renal disease over a median period of 47 months, despite chemotherapy in four patients. In one patient, monoclonal λLC deposits were observed on a follow-up kidney biopsy after 4 years., Conclusions: GN with isolated glomerular C3 deposits might represent an unusual complication of plasma cell dyscrasia, related to complement activation through an autoantibody activity of the monoclonal Ig against a CAP regulator protein.
- Published
- 2011
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39. TLR4- and TLR2-mediated B cell responses control the clearance of the bacterial pathogen, Leptospira interrogans.
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Chassin C, Picardeau M, Goujon JM, Bourhy P, Quellard N, Darche S, Badell E, d'Andon MF, Winter N, Lacroix-Lamandé S, Buzoni-Gatel D, Vandewalle A, and Werts C
- Subjects
- Animals, B-Lymphocyte Subsets pathology, Female, Genetic Predisposition to Disease, Inflammation Mediators metabolism, Inflammation Mediators physiology, Leptospira interrogans immunology, Leptospirosis pathology, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Signal Transduction genetics, Signal Transduction immunology, Toll-Like Receptor 2 deficiency, Toll-Like Receptor 2 genetics, Toll-Like Receptor 4 deficiency, Toll-Like Receptor 4 genetics, B-Lymphocyte Subsets immunology, B-Lymphocyte Subsets microbiology, Leptospira interrogans growth & development, Leptospirosis immunology, Leptospirosis microbiology, Toll-Like Receptor 2 physiology, Toll-Like Receptor 4 physiology
- Abstract
Leptospirosis is a widespread zoonosis caused by pathogenic Leptospira interrogans that are transmitted by asymptomatic infected rodents. Leptospiral lipoproteins and LPS have been shown to stimulate murine cells via TLRs 2 and 4. Host defense mechanisms remain obscure, although TLR4 has been shown to be involved in clearing Leptospira. In this study, we show that double (TLR2 and TLR4) knockout (DKO) mice rapidly died from severe hepatic and renal failure following Leptospira inoculation. Strikingly, the severe proinflammatory response detected in the liver and kidney from Leptospira-infected DKO mice appears to be independent of MyD88, the main adaptor of TLRs. Infection of chimeric mice constructed with wild-type and DKO mice, and infection of several lines of transgenic mice devoid of T and/or B lymphocytes, identified B cells as the crucial lymphocyte subset responsible for the clearance of Leptospira, through the early production of specific TLR4-dependent anti-Leptospira IgMs elicited against the leptospiral LPS. We also found a protective tissue compartmentalized TLR2/TLR4-mediated production of IFN-gamma by B and T lymphocytes, in the liver and kidney, respectively. In contrast, the tissue inflammation observed in Leptospira-infected DKO mice was further characterized to be mostly due to B lymphocytes in the liver and T cells in the kidney. Altogether these findings demonstrate that TLR2 and TLR4 play a key role in the early control of leptospirosis, but do not directly trigger the inflammation induced by pathogenic Leptospira.
- Published
- 2009
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40. The Na, K-ATPase alpha3-isoform specifically localizes in the Schmidt-Lanterman incisures of human nerve.
- Author
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Rigoard P, Tartarin F, Buffenoir K, Chaillou M, Fares M, D'Houtaud S, Wager M, Giot JP, Quellard N, Fernandez B, Lapierre F, and Maixent JM
- Subjects
- Amino Acid Sequence, Humans, Microscopy, Confocal, Microscopy, Fluorescence, Models, Molecular, Molecular Sequence Data, Myelin Sheath enzymology, Protein Conformation, Schwann Cells enzymology, Sodium-Potassium-Exchanging ATPase analysis, Sural Nerve enzymology
- Abstract
Introduction: To our knowledge, there is little reference in literature with regards to alpha3-isoform of Na+,K+-ATPase in human peripheral nerves. The aim of this study was to determine the expression of the neuronal alpha3-isoform of Na+,K+-ATPase in human sural nerves from patients with a permanent medullary central nervous system injury., Materials and Methods: We studied the immunolocalization of alpha3-isoform of Na+,K+-ATPase using a polyclonal antibody against the amino sequence near the phosphorylation site of the alpha3-isoforms of Na+,K+-ATPase using immunohistochemistry and confocal laser scanning microscopy. An antibody specific for alpha2-isoform of Na+,K+-ATPase was used to label the Schwann cells., Results: Morphometric analysis of longitudinal section of human sural nerves showed that the alpha3-isoform of Na+,K+-ATPase was distributed along the length of axolemma. The myelin sheath of the Schwann cells showed clearly a distribution of alpha3- but not alpha2-isoforms of Na+,K+-ATPase at the level of Schmidt-Lanterman incisures., Conclusion: The human sural nerve shows a specific localization of the Na+,K+-ATPase alpha3-isoform in the Schmidt-Lanterman incisures of Schwann cells in addition to its localization in axonal membranes.
- Published
- 2007
41. Fanconi's syndrome induced by a monoclonal Vkappa3 light chain in Waldenstrom's macroglobulinemia.
- Author
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Bridoux F, Sirac C, Hugue V, Decourt C, Thierry A, Quellard N, Abou-Ayache R, Goujon JM, Cogné M, and Touchard G
- Subjects
- Aged, Amino Acid Sequence, Antibodies, Monoclonal genetics, Antibodies, Monoclonal urine, Base Sequence, Biopsy, Bone Marrow pathology, Fanconi Syndrome etiology, Fanconi Syndrome pathology, Genes, Immunoglobulin, Germ-Line Mutation, Humans, Immunoglobulin M blood, Immunoglobulin Variable Region genetics, Immunoglobulin Variable Region immunology, Immunoglobulin kappa-Chains genetics, Kidney pathology, Kidney Failure, Chronic etiology, Kidney Tubules, Proximal immunology, Kidney Tubules, Proximal pathology, Male, Molecular Sequence Data, Protein Structure, Tertiary, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Trypsin metabolism, Waldenstrom Macroglobulinemia genetics, Waldenstrom Macroglobulinemia immunology, Antibodies, Monoclonal immunology, Fanconi Syndrome immunology, Immunoglobulin kappa-Chains immunology, Waldenstrom Macroglobulinemia complications
- Abstract
Fanconi's syndrome (FS) is a disorder of sodium-dependent proximal tubule reabsorption, which may complicate plasma cell disorders producing a free monoclonal light chain (LC). FS often occurs in the setting of smoldering myeloma and features cytoplasmic crystalline inclusions of monoclonal kappa LC in proximal tubular cells and malignant plasma cells. Although the clinical and pathological presentation may vary, including lack of crystal formation, monoclonal kappa LCs that underlie FS show a striking genetic and biochemical homogeneity: they almost always belong to the Vkappa1 subgroup of variability and originate from 2 germline genes, O2/O12 or O8/O18. Their variable domain sequences present unusual hydrophobic residues, responsible for the resistance to proteolysis, which leads to LC accumulation in the endocytic compartment of proximal tubule cells. We report a patient with slowly progressive Waldenstrom's macroglobulinemia and full-blown FS with accumulation of a monoclonal kappa LC within proximal tubules, but no detectable crystalline organization. This LC, which belonged to the unusual Vkappa3 subgroup and derived from the L2/L16 germline gene, showed no common substitution with previously described FS kappaI LC and was sensitive to trypsin digestion. These data show that molecular and biochemical characteristics of kappa LCs in patients with FS are more heterogeneous than initially suspected. Mechanisms other than resistance of LCs to endosomal proteolysis probably are involved in the pathogenesis of FS-associated plasma cell dyscrasias.
- Published
- 2005
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42. [Ultrastructural study of epiretinal membrane stained by trypan blue: 15 case reports].
- Author
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Balayre S, Boissonnot M, Fernandez B, Quellard N, Babin P, and Dighiero P
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- Aged, Aged, 80 and over, Female, Humans, Male, Middle Aged, Prospective Studies, Trypan Blue, Retina ultrastructure
- Abstract
Introduction: Idiopathic epiretinal membrane results from detachment of the posterior hyaloid and is believed to be related to naturally occurring defects in the internal limiting membrane (ILM) of the retina. Vitrectomy and peeling are the treatment of choice. Trypan blue 0.15% (TB) stains epiretinal membrane and internal limiting membrane. It allows selective and complete removal, facilitating surgery, with less retinal damage. An ultrastructural study was conducted showing ultrastructural features of idiopathic epiretinal membranes (ERM) and those of the internal limiting membrane and its connections with the retinal side., Material and Methods: After pars plana vitrectomy and induction of posterior vitreous detachment, 0.2 ml TB 0.15% was injected over the ERM in an air-filled eye. The stained tissue was peeled with intraocular forceps. Specimens were at once collected in 4% glutaraldehyde for a transmission electron microscopy study., Results: TB may allow complete and easier ERM and ILM peeling. The staining does not present toxic effects. The major cellular contingent is represented by glial cells, participating actively in neocollagen synthesis. Their presence supports the hypothesis of a migratory movement of retinal cells toward the vitreoretinal side., Conclusion: The presence of an intact internal limiting membrane, the absence of optical fibers belonging to the under retina, and the absence of any sign of apoptosis make TB a useful staining agent for ERM and ILM peeling.
- Published
- 2005
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43. Expression, localization, and thyrotropin regulation of cathepsin D in human thyroid tissues.
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Métayé T, Kraimps JL, Goujon JM, Fernandez B, Quellard N, Ingrand P, Barbier J, and Bégon F
- Subjects
- Adult, Aged, Cells, Cultured, Female, Humans, Immunoblotting, Immunohistochemistry, Isoenzymes metabolism, Male, Middle Aged, Reference Values, Thyroid Diseases metabolism, Thyroid Gland cytology, Tissue Distribution, Cathepsin D metabolism, Thyroid Gland drug effects, Thyroid Gland metabolism, Thyrotropin pharmacology
- Abstract
Enzymatic activity and isoform expression of cathepsin D (cath D) were studied in 107 cytosols from various human thyroid tissues including 21 normal tissues, 12 cold benign nodules, 17 toxic adenomas, 22 samples from Graves' disease patients, and 35 thyroid carcinomas. Cath D assay was optimized for human thyroid tissues. We found that mean cath D specific activities, expressed as units per milligrams protein minus thyroglobulin, were higher in carcinomas (P = 0.0001), toxic adenomas (P = 0.0001), and specimens from Graves' disease patients (P = 0.0001) than in normal thyroid tissues. Mean cath D activity in carcinomas was also significantly different from that in cold benign nodules (P < 0.001) and Graves' disease tissues (P < 0.05) but not from that of toxic adenomas. To determine possible mechanisms by which the observed increase in cath D activity might be regulated, we used Western blotting to measure relative amounts of cath D isoforms in the various thyroid tissues. We found that the 31-kDa major processing form of cath D was significantly increased in carcinomas and toxic adenomas compared with normal tissues (P < 0.01), cold benign nodules (P < 0.05), and Graves' disease tissues (P < 0.05). A positive correlation of cath D activity with relative expression of the 31-kDa form (r = 0.67, P = 0.0001) was observed in 104 thyroid cytosols. These data demonstrate a deregulation at the protein level, with resulting increases in cath D activity. Immunogold labeling of cath D showed particle concentration in lysosomes or phagosomes in both normal follicles and papillary carcinoma cells, indicating that cath D localization was not altered by malignant transformation in human thyroid cells. TSH induced cath D synthesis and secretion in extracellular fluid of normal human thyroid cells in primary culture; TSH had little effect on intracellular cath D level. In conclusion, TSH-induced cath D synthesis may explain high cath D levels in Graves' disease tissues and toxic adenomas, because these tissues possess a permanently stimulated cAMP transduction pathway. Furthermore, the overexpression of cath D in thyroid carcinomas in comparison with normal controls adds further arguments for the potential role of cath D in tumor growth and metastasis.
- Published
- 1997
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44. Detection of myosin immunoanalogue in the yeast Candida albicans.
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Ghazali M, Rodier MH, el Moudni B, Quellard N, and Jacquemin JL
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- Candida albicans immunology, Candida albicans ultrastructure, Cell Compartmentation, Cross Reactions, Fluorescent Antibody Technique, Immunoblotting, Microscopy, Immunoelectron, Molecular Weight, Myosins immunology, Candida albicans chemistry, Myosins isolation & purification
- Abstract
Detection and localization of myosin immunoanalogue protein in the yeast Candida albicans were achieved by immunoblotting, indirect immunofluorescence assay, and immunoelectron microscopy. A polypeptide with an M(r) about 110,000, from cytosolic extract and insoluble fraction in the corresponding membrane pellet, was reacted with polyclonal and monoclonal antibodies raised against vertebrate muscle myosin. This protein was located by immunofluorescence and immunoelectron microscopy in the cell cortex along the plasmalemma, in the cytoplasm, and in the septum corresponding to bud scar region situated between the yeast-mother cell and the bud.
- Published
- 1995
- Full Text
- View/download PDF
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