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32 results on '"Quedenau, Claudia"'

2. Long-read sequencing identifies a common transposition haplotype predisposing for CLCNKB deletions

Author

Tschernoster, Nikolai, Erger, Florian, Kohl, Stefan, Reusch, Björn, Wenzel, Andrea, Walsh, Stephen, Thiele, Holger, Becker, Christian, Franitza, Marek, Bartram, Malte P., Kömhoff, Martin, Schumacher, Lena, Kukat, Christian, Borodina, Tatiana, Quedenau, Claudia, Nürnberg, Peter, Rinschen, Markus M, Driller, Jan H., Pedersen, Bjørn P., Schlingmann, Karl P., Hüttel, Bruno, Bockenhauer, Detlef, Beck, Bodo, and Altmüller, Janine

Published
2023
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3. Increased risk of severe clinical course of COVID-19 in carriers of HLA-C*04:01

Author

Weiner, January, Suwalski, Phillip, Holtgrewe, Manuel, Rakitko, Alexander, Thibeault, Charlotte, Müller, Melina, Patriki, Dimitri, Quedenau, Claudia, Krüger, Ulrike, Ilinsky, Valery, Popov, Iaroslav, Balnis, Joseph, Jaitovich, Ariel, Helbig, Elisa T, Lippert, Lena J, Stubbemann, Paula, Real, Luis M, Macías, Juan, Pineda, Juan A, Fernandez-Fuertes, Marta, Wang, Xiaomin, Karadeniz, Zehra, Saccomanno, Jacopo, Doehn, Jan-Moritz, Hübner, Ralf-Harto, Hinzmann, Bernd, Salvo, Mauricio, Blueher, Anja, Siemann, Sandra, Jurisic, Stjepan, Beer, Juerg H, Rutishauser, Jonas, Wiggli, Benedikt, Schmid, Hansruedi, Danninger, Kathrin, Binder, Ronald, Corman, Victor M, Mühlemann, Barbara, Arkal, Rao Arjun, Fragiadakis, Gabriela K, Mick, Eran, COMET, Consortium, Calfee, Carolyn S, Erle, David J, Hendrickson, Carolyn M, Kangelaris, Kirsten N, Krummel, Matthew F, Woodruff, Prescott G, Langelier, Charles R, Venkataramani, Urmila, García, Federico, Zyla, Joanna, Drosten, Christian, Alice, Braun, Jones, Terry C, Suttorp, Norbert, Witzenrath, Martin, Hippenstiel, Stefan, Zemojtel, Tomasz, Skurk, Carsten, Poller, Wolfgang, Borodina, Tatiana, Pa-COVID, Study Group, Ripke, Stephan, Sander, Leif E, Beule, Dieter, Landmesser, Ulf, Guettouche, Toumy, Kurth, Florian, and Heidecker, Bettina

Subjects
Biomedical and Clinical Sciences, Clinical Sciences, Coronaviruses, Emerging Infectious Diseases, Human Genome, Clinical Research, Infectious Diseases, Genetics, 2.1 Biological and endogenous factors, 2.5 Research design and methodologies (aetiology), Good Health and Well Being, SARS-CoV-2, COVID-19, Human Leukocyte Antigen&nbsp, intubation, Human Leukocyte Antigen, Clinical sciences, Health services and systems, Public health
Abstract

BackgroundSince the beginning of the coronavirus disease 2019 (COVID-19) pandemic, there has been increasing urgency to identify pathophysiological characteristics leading to severe clinical course in patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Human leukocyte antigen alleles (HLA) have been suggested as potential genetic host factors that affect individual immune response to SARS-CoV-2. We sought to evaluate this hypothesis by conducting a multicenter study using HLA sequencing.MethodsWe analyzed the association between COVID-19 severity and HLAs in 435 individuals from Germany (n = 135), Spain (n = 133), Switzerland (n = 20) and the United States (n = 147), who had been enrolled from March 2020 to August 2020. This study included patients older than 18 years, diagnosed with COVID-19 and representing the full spectrum of the disease. Finally, we tested our results by meta-analysing data from prior genome-wide association studies (GWAS).FindingsWe describe a potential association of HLA-C*04:01 with severe clinical course of COVID-19. Carriers of HLA-C*04:01 had twice the risk of intubation when infected with SARS-CoV-2 (risk ratio 1.5 [95% CI 1.1-2.1], odds ratio 3.5 [95% CI 1.9-6.6], adjusted p-value = 0.0074). These findings are based on data from four countries and corroborated by independent results from GWAS. Our findings are biologically plausible, as HLA-C*04:01 has fewer predicted bindings sites for relevant SARS-CoV-2 peptides compared to other HLA alleles.InterpretationHLA-C*04:01 carrier state is associated with severe clinical course in SARS-CoV-2. Our findings suggest that HLA class I alleles have a relevant role in immune defense against SARS-CoV-2.FundingFunded by Roche Sequencing Solutions, Inc.

Published
2021

4. Increased risk of severe clinical course of COVID-19 in carriers of HLA-C*04:01

Author

Weiner, January, 3rd, Suwalski, Phillip, Holtgrewe, Manuel, Rakitko, Alexander, Thibeault, Charlotte, Müller, Melina, Patriki, Dimitri, Quedenau, Claudia, Krüger, Ulrike, Ilinsky, Valery, Popov, Iaroslav, Balnis, Joseph, Jaitovich, Ariel, Helbig, Elisa T, Lippert, Lena J, Stubbemann, Paula, Real, Luis M, Macías, Juan, Pineda, Juan A, Fernandez-Fuertes, Marta, Wang, Xiaomin, Karadeniz, Zehra, Saccomanno, Jacopo, Doehn, Jan-Moritz, Hübner, Ralf-Harto, Hinzmann, Bernd, Salvo, Mauricio, Blueher, Anja, Siemann, Sandra, Jurisic, Stjepan, Beer, Juerg H., Rutishauser, Jonas, Wiggli, Benedikt, Schmid, Hansruedi, Danninger, Kathrin, Binder, Ronald, Corman, Victor M, Mühlemann, Barbara, Arjun Arkal, Rao, Fragiadakis, Gabriela K., Mick, Eran, COMET, Consortium, Calfee, Carolyn S., Erle, David J., Hendrickson, Carolyn M., Kangelaris, Kirsten N., Krummel, Matthew F., Woodruff, Prescott G., Langelier, Charles R., Venkataramani, Urmila, García, Federico, Zyla, Joanna, Drosten, Christian, Alice, Braun, Jones, Terry C, Suttorp, Norbert, Witzenrath, Martin, Hippenstiel, Stefan, Zemojtel, Tomasz, Skurk, Carsten, Poller, Wolfgang, Borodina, Tatiana, Pa-COVID, Study Group, Ripke, Stephan, Sander, Leif E, Beule, Dieter, Landmesser, Ulf, Guettouche, Toumy, Kurth, Florian, and Heidecker, Bettina

Published
2021
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8. SARS-CoV-2 infection dynamics revealed by wastewater sequencing analysis and deconvolution

Author

Schumann, Vic-Fabienne, primary, de Castro Cuadrat, Rafael Ricardo, additional, Wyler, Emanuel, additional, Wurmus, Ricardo, additional, Deter, Aylina, additional, Quedenau, Claudia, additional, Dohmen, Jan, additional, Faxel, Miriam, additional, Borodina, Tatiana, additional, Blume, Alexander, additional, Freimuth, Jonas, additional, Meixner, Martin, additional, Grau, José Horacio, additional, Liere, Karsten, additional, Hackenbeck, Thomas, additional, Zietzschmann, Frederik, additional, Gnirss, Regina, additional, Böckelmann, Uta, additional, Uyar, Bora, additional, Franke, Vedran, additional, Barke, Niclas, additional, Altmüller, Janine, additional, Rajewsky, Nikolaus, additional, Landthaler, Markus, additional, and Akalin, Altuna, additional

Published
2022
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9. De Novo-Whole Genome Assembly of the Roborovski Dwarf Hamster (Phodopus roborovskii) Genome: An Animal Model for Severe/Critical COVID-19

Author

Andreotti, Sandro, primary, Altmüller, Janine, additional, Quedenau, Claudia, additional, Borodina, Tatiana, additional, Nouailles, Geraldine, additional, Teixeira Alves, Luiz Gustavo, additional, Landthaler, Markus, additional, Bieniara, Maximilian, additional, Trimpert, Jakob, additional, and Wyler, Emanuel, additional

Published
2022
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10. De Novo-Whole Genome Assembly of the Roborovski Dwarf Hamster (Phodopus roborovskii) Genome: An Animal Model for Severe/Critical COVID-19

Author

Andreotti, Sandro, Altmueller, Janine, Quedenau, Claudia, Borodina, Tatiana, Nouailles, Geraldine, Teixeira Alves, Luiz Gustavo, Landthaler, Markus, Bieniara, Maximilian, Trimpert, Jakob, Wyler, Emanuel, Andreotti, Sandro, Altmueller, Janine, Quedenau, Claudia, Borodina, Tatiana, Nouailles, Geraldine, Teixeira Alves, Luiz Gustavo, Landthaler, Markus, Bieniara, Maximilian, Trimpert, Jakob, and Wyler, Emanuel

Abstract

The Roborovski dwarf hamster Phodopus roborovskii belongs to the Phodopus genus, one of the seven within Cricetinae subfamily. Like other rodents such as mice, rats, or ferrets, hamsters can be important animal models for a range of diseases. Whereas the Syrian hamster from the genus Mesocricetus is now widely used as a model for mild-to-moderate coronavirus disease 2019, Roborovski dwarf hamster shows a severe-to-lethal course of disease upon infection with the novel human coronavirus severe acute respiratory syndrome coronavirus 2.

Published
2022

11. Amorfrutins are potent antidiabetic dietary natural products

Author

Weidner, Christopher, de Groot, Jens C., Prasad, Aman, Freiwald, Anja, Quedenau, Claudia, Kliem, Magdalena, Witzke, Annabell, Kodelja, Vitam, Han, Chung-Ting, Giegold, Sascha, Baumann, Matthias, Klebl, Bert, Siems, Karsten, Müller-Kuhrt, Lutz, Schürmann, Annette, Schüler, Rita, Pfeiffer, Andreas F. H., Schroeder, Frank C., Büssow, Konrad, and Sauer, Sascha

Published
2012

12. MALE SEX, HLA-C*04:01 AND COVID-19: A RISKY CONSTELLATION

Author

Suwalski, Phillip, primary, Patriki, Dimitri, additional, Quedenau, Claudia, additional, Borodina, Tatiana, additional, Beer, Juerg H., additional, Wiggli, Benedikt, additional, Guettouche, Toumy, additional, Landmesser, Ulf, additional, and Heidecker, Bettina, additional

Published
2022
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13. SARS-CoV-2 infection dynamics revealed by wastewater sequencing analysis and deconvolution

Author

Schumann, Vic-Fabienne, primary, de Castro Cuadrat, Rafael Ricardo, additional, Wyler, Emanuel, additional, Wurmus, Ricardo, additional, Deter, Aylina, additional, Quedenau, Claudia, additional, Dohmen, Jan, additional, Faxel, Miriam, additional, Borodina, Tatiana, additional, Blume, Alexander, additional, Meixner, Martin, additional, Grau, José Horacio, additional, Liere, Karsten, additional, Hackenbeck, Thomas, additional, Zietzschmann, Frederik, additional, Gnirss, Regina, additional, Böckelmann, Uta, additional, Uyar, Bora, additional, Franke, Vedran, additional, Barke, Niclas, additional, Altmüller, Janine, additional, Rajewsky, Nikolaus, additional, Landthaler, Markus, additional, and Akalin, Altuna, additional

Published
2021
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14. De Novo Whole Genome Assembly of the Roborovski Dwarf Hamster (Phodopus roborovskii) Genome, an Animal Model for Severe/Critical COVID-19

Author

Andreotti, Sandro, primary, Altmüller, Janine, additional, Quedenau, Claudia, additional, Borodina, Tatiana, additional, Nouailles, Geraldine, additional, Alves, Luiz Gustavo Teixeira, additional, Landthaler, Markus, additional, Bieniara, Maximilian, additional, Trimpert, Jakob, additional, and Wyler, Emanuel, additional

Published
2021
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15. Polylox barcoding reveals haematopoietic stem cell fates realized in vivo

Author

Pei, Weike, Feyerabend, Thorsten B., Rssler, Jens, Wang, Xi, Postrach, Daniel, Busch, Katrin, Rode, Immanuel, Klapproth, Kay, Dietlein, Nikolaus, Quedenau, Claudia, Chen, Wei, Sauer, Sascha, Wolf, Stephan, Hfer, Thomas, and Rodewald, Hans-Reimer

Subjects
Hematopoietic stem cells -- Physiological aspects, DNA barcoding -- Analysis, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Author(s): Weike Pei [1]; Thorsten B. Feyerabend [1]; Jens Rssler [2]; Xi Wang [2, 3]; Daniel Postrach [1]; Katrin Busch [1]; Immanuel Rode [1]; Kay Klapproth [1]; Nikolaus Dietlein [1]; [...]

Published
2017
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18. Resolving Fates and Single-Cell Transcriptomes of Hematopoietic Stem Cell Clones by PolyloxExpress Barcoding

Author

Pei, Weike, primary, Shang, Fuwei, additional, Wang, Xi, additional, Fanti, Ann-Kathrin, additional, Greco, Alessandro, additional, Busch, Katrin, additional, Klapproth, Kay, additional, Zhang, Qin, additional, Quedenau, Claudia, additional, Sauer, Sascha, additional, Feyerabend, Thorsten B., additional, Höfer, Thomas, additional, and Rodewald, Hans-Reimer, additional

Published
2020
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19. Resolving fate and transcriptome of hematopoietic stem cell clones

Author

Pei, Weike, primary, Shang, Fuwei, additional, Wang, Xi, additional, Fanti, Ann-Kathrin, additional, Greco, Alessandro, additional, Busch, Katrin, additional, Klapproth, Kay, additional, Zhang, Qin, additional, Quedenau, Claudia, additional, Sauer, Sascha, additional, Feyerabend, Thorsten B., additional, Höfer, Thomas, additional, and Rodewald, Hans-Reimer, additional

Published
2020
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22. DNA sonication inverse PCR for genome scale analysis of uncharacterized flanking sequences.

Author

Alquezar‐Planas, David E., Löber, Ulrike, Cui, Pin, Quedenau, Claudia, Chen, Wei, Greenwood, Alex D., and Johnston, Susan

Subjects
SONICATION, MOBILE genetic elements, ETHNOBIOLOGY, BIOLOGICAL systems, DNA, GENOMES
Abstract

There are few available tools to comprehensively and economically identify uncharacterized flanking regions that are not extremely labour intensive and which exploit the advantages of emerging long‐read sequencing platforms.We describe SIP; a sonication‐based inverse PCR high‐throughput sequencing strategy to investigate uncharacterized flanking region sequences, including those flanking mobile DNA. SIP combines unbiased fragmentation by sonication and target enrichment by coupling outward facing PCR priming with long‐read sequencing technologies.We demonstrate the effectiveness of SIP by determining retroviral integrations which are high copy and challenging to characterize. We further describe SIP's workflow, examine retroviral (proviral) enrichment and characterize viral structural variants identified. When SIP was coupled with long‐read sequencing using the PacBio RS II platform, proviral integration was extensively characterized at high sequence depth per integration. By interrogating the sequence data, we were also able to test several intrinsic factors including SIP's propensity to form chimeric sequences and adapter ligation efficiencies.SIP is an adaption of a traditional molecular biology technique that can be used to characterize any unknown genomic flanking sequence or to extend any sequence for which only minimal sequence information is available. SIP can be applied broadly to study complex biological systems such as mobile genetic elements with high throughput. [ABSTRACT FROM AUTHOR]

Published
2021
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24. Integrative analysis revealed the molecular mechanism underlying RBM10-mediated splicing regulation: RBM10 mediated alternative splicing

Author

Wang, Yang, Wang, Yongbo, Kalscheuer, Vera, Wieczorek, Dagmar, Hu, Yuhui, Murakawa, Yasuhiro, Chen, Wei, Maaskola, Jonas, Hu, Hao, Jens, Marvin, Quedenau, Claudia, Landthaler, Markus, Fröhler, Sebastian, Ma, Yunxia, and Gogol-Döring, Andreas

Abstract

RBM10 encodes an RNA binding protein. Mutations in RBM10 are known to cause multiple congenital anomaly syndrome in male humans, the TARP syndrome. However, the molecular function of RBM10 is unknown. Here we used PAR-CLIP to identify thousands of binding sites of RBM10 and observed significant RBM10–RNA interactions in the vicinity of splice sites. Computational analyses of binding sites as well as loss-of-function and gain-of-function experiments provided evidence for the function of RBM10 in regulating exon skipping and suggested an underlying mechanistic model, which could be subsequently validated by minigene experiments. Furthermore, we demonstrated the splicing defects in a patient carrying an RBM10 mutation, which could be explained by disrupted function of RBM10 in splicing regulation. Overall, our study established RBM10 as an important regulator of alternative splicing, presented a mechanistic model for RBM10-mediated splicing regulation and provided a molecular link to understanding a human congenital disorder.

Published
2013
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25. Neural circular RNAs are derived from synaptic genes and regulated by development and plasticity

Author

You, Xintian, primary, Vlatkovic, Irena, additional, Babic, Ana, additional, Will, Tristan, additional, Epstein, Irina, additional, Tushev, Georgi, additional, Akbalik, Güney, additional, Wang, Mantian, additional, Glock, Caspar, additional, Quedenau, Claudia, additional, Wang, Xi, additional, Hou, Jingyi, additional, Liu, Hongyu, additional, Sun, Wei, additional, Sambandan, Sivakumar, additional, Chen, Tao, additional, Schuman, Erin M, additional, and Chen, Wei, additional

Published
2015
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26. Structural and functional characterization of human Iba proteins

Author

Schulze, Jörg O, Quedenau, Claudia, Roske, Yvette, Adam, Thomas, Schüler, Herwig, Behlke, Joachim, Turnbull, Andrew P, Sievert, Volker, Scheich, Christoph, Mueller, Uwe, Heinemann, Udo, Büssow, Konrad, and Max Delbrück Center for Molecular Medicine, Berlin, Germany.

Subjects
Models, Molecular, Calcium-Binding Proteins, Microfilament Proteins, Molecular Sequence Data, Crystallography, X-Ray, Actins, DNA-Binding Proteins, Hela Cells, Humans, Calcium, Amino Acid Sequence, Shigella, Dimerization, Sequence Alignment
Abstract

Iba2 is a homolog of ionized calcium-binding adapter molecule 1 (Iba1), a 17-kDa protein that binds and cross-links filamentous actin (F-actin) and localizes to membrane ruffles and phagocytic cups. Here, we present the crystal structure of human Iba2 and its homodimerization properties, F-actin cross-linking activity, cellular localization and recruitment upon bacterial invasion in comparison with Iba1. The Iba2 structure comprises two central EF-hand motifs lacking bound Ca2+. Iba2 crystallized as a homodimer stabilized by a disulfide bridge and zinc ions. Analytical ultracentrifugation revealed a different mode of dimerization under reducing conditions that was independent of Ca2+. Furthermore, no binding of Ca2+ up to 0.1 mM was detected by equilibrium dialysis. Correspondingly, Iba EF-hand motifs lack residues essential for strong Ca2+ coordination. Sedimentation experiments and microscopy detected pronounced, indistinguishable F-actin binding and cross-linking activity of Iba1 and Iba2 with induction of F-actin bundles. Fluorescent Iba fusion proteins were expressed in HeLa cells and co-localized with F-actin. Iba1 was recruited into cellular projections to a larger extent than Iba2. Additionally, we studied Iba recruitment in a Shigella invasion model that induces cytoskeletal rearrangements. Both proteins were recruited into the bacterial invasion zone and Iba1 was again concentrated slightly higher in the cellular extensions.

Published
2008

27. Integrative analysis revealed the molecular mechanism underlying RBM 10‐mediated splicing regulation

Author

Wang, Yongbo, primary, Gogol‐Döring, Andreas, additional, Hu, Hao, additional, Fröhler, Sebastian, additional, Ma, Yunxia, additional, Jens, Marvin, additional, Maaskola, Jonas, additional, Murakawa, Yasuhiro, additional, Quedenau, Claudia, additional, Landthaler, Markus, additional, Kalscheuer, Vera, additional, Wieczorek, Dagmar, additional, Wang, Yang, additional, Hu, Yuhui, additional, and Chen, Wei, additional

Published
2013
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28. Structural Basis for Complex Formation between Human IRSp53 and the Translocated Intimin Receptor Tir of Enterohemorrhagic E. coli

Author

de Groot, Jens C., primary, Schlüter, Kai, additional, Carius, Yvonne, additional, Quedenau, Claudia, additional, Vingadassalom, Didier, additional, Faix, Jan, additional, Weiss, Stefanie M., additional, Reichelt, Joachim, additional, Standfuß-Gabisch, Christine, additional, Lesser, Cammie F., additional, Leong, John M., additional, Heinz, Dirk W., additional, Büssow, Konrad, additional, and Stradal, Theresia E.B., additional

Published
2011
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29. Expression of protein complexes using multiple Escherichia coli protein co-expression systems: A benchmarking study

Author

Busso, Didier, primary, Peleg, Yoav, additional, Heidebrecht, Tatjana, additional, Romier, Christophe, additional, Jacobovitch, Yossi, additional, Dantes, Ada, additional, Salim, Loubna, additional, Troesch, Edouard, additional, Schuetz, Anja, additional, Heinemann, Udo, additional, Folkers, Gert E., additional, Geerlof, Arie, additional, Wilmanns, Matthias, additional, Polewacz, Andrea, additional, Quedenau, Claudia, additional, Büssow, Konrad, additional, Adamson, Rachel, additional, Blagova, Elena, additional, Walton, Julia, additional, Cartwright, Jared L., additional, Bird, Louise E., additional, Owens, Raymond J., additional, Berrow, Nick S., additional, Wilson, Keith S., additional, Sussman, Joel L., additional, Perrakis, Anastassis, additional, and Celie, Patrick H.N., additional

Published
2011
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32. A catalog of human cDNA expression clones and its application to structural genomics

Author

Büssow, Konrad, Quedenau, Claudia, Sievert, Volker, Tischer, Janett, Scheich, Christoph, Seitz, Harald, Hieke, Brigitte, Niesen, Frank, Götz, Frank, Harttig, Ulrich, and Lehrach, Hans

Abstract

We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.

Published
2004
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