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Large-scale open-pit coal mining will cause ecological problems such as land excavation and occupation, groundwater drainage, soil erosion, vegetation destruction, air pollution, and regional landscape damage. The selection of existing open-pit coal mine ecological restoration technologies is relatively limited, and there is a lack of systematic research on ecological total factors. In this paper, the concept of “damage reduction mining and systematic restoration” was proposed, and the connotation and technical approach of ecological mining of open-pit coal mine were expounded. The key scientific issues and basic theoretical framework of ecological mining of open-pit coal were proposed. Then the whole life cycle ecological impairment mining technology and the coordination system restoration technology of ecological elements (water, soil, vegetation, landscape) of “pre-harvest ecological design-mining during mining loss-postharvest ecological restoration”were developed. At last, the theory and technical system of open-pit coal mine ecological mining was established, and a generalizable and replicable open-pit mine ecological mining mode was formed. The results show that in the semi-arid area, the ecological restoration technology has significant effects. The area of land excavation and occupation loss is reduced by 60003 m2 every year, the ecological restoration period of the dump site is advanced by more than one year, and the vegetation coverage rate increases by 41.78% compared with the background value in the semi-arid area of the Shengli open-pit mine demonstration area. A surface reservoir with a water storage capacity of 400 000 cubic meters has been built, and the seasonal and graded utilization of mine water has been realized. In the extremely cold area of Baorixiler, the area occupied by land excavation is reduced by 106 672 m2 per year, the number of new species has increased from 14 to 21, the soil moisture content has increased by 10%, the annual soil erosion rate has decreased by 59.6%, and the vegetation coverage rate has increased by 37.96% compared with the background value in the demonstration area of the Baorixiler open-pit mine. An open-pit coal mine underground reservoir with a water storage capacity of 1.22 million square meters has been built, realizing the “winter storage and summer use” of mine water.

In view of the large-scale and efficient coal mining in ecologically fragile areas, which causes a large scope, long cycle and high intensity of ecological impacts, and the lack of a systematic technology that integrates the whole cycle of mining, source reduction and ecological elements, which leads to high costs, low efficiency and poor results in the ecological restoration of mining areas. This paper puts forward the concept of ecological protection coal mining, the damage conduction pathway of surface mining and the damage conduction model of shaft mining, the quantitative analysis method of ecological damage and the decision-making method of ecological restoration in mining areas. It has developed land-saving and loss-reducing mining for open pit mines, ecological stratigraphic reconstruction, distributed water resources collection, storage and use, and synergistic restoration technologies based on the "ecological restoration window period". It has researched and developed technologies for damage conduction control in well mining, ecological function restoration of aquifers, construction of assembled wedge-shaped groundwater reservoir dams, and ecological restoration of subsidence zones in time zones. An ecologically protected open pit mining demonstration project has been completed in the mengdong mining area, with the vegetation cover in the baorixile open pit mine demonstration area increasing from 30% to 59% of the pre-exploitation area, and that in the shengli open pit mine demonstration area from 5% to 39% of the pre-exploitation area. A demonstration project for ecologically protected coal mining in the shengdong wells has been completed, with vegetation cover increasing from less than 10% to 70 % of the pre-exploitation area. The research results provide scientific and technological support for eco-protective coal mining and have wide application and promotion value.

As a commonly used traditional Chinese medicine formula for treating rheumatoid arthritis (RA), Sishen Decoction (SSD) has anti-inflammatory, analgesic and swelling relief effects. However, at present, the pharmacodynamic basis of SSD and its mechanism of treating RA have not been clarified, and further research is needed. Analyzing the pharmacological basis of SSD was the aim of our study and further elucidate its therapeutic mechanism and potential targets for treating RA. LC‒MS was used to identify the high content and characteristic chemical components of SSD. On this basis, a network of pharmacological analysis was established between the chemical structure and RA. According to the predicted possible pathways and targets, in vivo pharmacodynamic experiments and related pathway analysis were conducted. Finally, the possible targets and mechanisms of SSD in treating RA were analyzed. Identified 78 compounds from SSD by LC-MS, including 23 flavonoids, 19 phenolic acids, 9 monoterpenoids and 26 other compounds. Network pharmacological analysis based on pharmacodynamic substances revealed that the most likely interaction pathway between SSD and RA was the PI3K/AKT/mTOR pathway. Foot swelling and inflammatory factors (IL-6, IL-10, IL-18, TGF, TNF-α, VEGF) in model mice were shown to be significantly improved in vivo. WB and qPCR experiments proved that SSD could significantly regulate the pathway of PI3K/AKT/mTOR. The interaction between SSD and AKT target was further analyzed by multispectroscopy. This study revealed that SSD alleviates RA by regulating the pathway of PI3K/AKT/mTOR and preliminarily revealed the pharmacodynamic mechanism of SSD for the first time.

The coal mining changes the overburden boundary conditions and causes the redistribution of the in-situ stress and the deformation, movement, fracture of overburden rock. The damage conducts from the bottom to top in overburden and causes the damage of aquifers, surface appurtenances, and mining area ecosystem. Besides, while the modern mining ensures the energy demand of social development, it also causes great damage to groundwater , ecological environment and construction/construction facilities in mining areas. In order to solve the problem of large-scale coal mining damage and the safe coordination of protected objects in the mining area, based on the systematic analysis of the damage reduction mining theory and technology and long-term practice worldwide, according to the concept of “source damage reduction” and “pre-mining source control, mining optimization reduction and post-mining collaborative repair” for the entire mining process reduction, a pre-mining, mid-mining, post-mining reduction technology is proposed as the main measures for reducing mining damage throughout the entire mining process, which includes the three types and four technologies: “source reduction technology based on mining process parameter optimization, hard rock pre-fracturing-soft rock grouting modification and postmining water conduction fracture sealing and water control technology, as well as maintaining the stability of the overburden bearing structure during mining”. And the theoretical and technical system for reducing mining damage in underground coal mines has been constructed, and the focus and direction of research on reducing mining damage has also been clarified. In terms of mining damage monitoring, due to the significant spatiotemporal differences in monitoring technology, it is difficult to achieve a synchronous monitoring of surface deformation and cracks in the same space-time, as well as an integrated monitoring of multiple elements of the surface ecological environment. Therefore, it is still necessary to propose the targeted methods for correcting and processing asynchronous data from the multi-source monitoring of coal mining induced damage. In terms of damage conduction theory, based on the theory of overburden damage conduction, a mining damage field model is proposed from the perspective of energy indicators. Combined with the differences in the combination of soft-hard rock layers and the media of rock-soil layers, a unified damage field quantity and cross interface conduction theory for the multiple factor indicators of “overburden-aquifer-surface-ecology” is proposed. The 12401 longwall face of the Shangwan coalmine has adopted the measures of pre-mining parameter optimization and grouting in the caving zone during mining, the maximum surface settlement amount has been relatively reduced by 25%, and the surface cracks have been adjusted from step-like cracks to dynamic small cracks. In addition, since the technical implementation at the Shendong mining area in 2010, the vegetation coverage has increased from an average of 31% to a maximum of 74.1%.

The northern sand-proof belt is the key zone for preventing and controlling desertification and sandification in China, and it is the core area of the “Three Norths” project, which is characterized by infertile soil, high wind speed, drought and water shortage, sparse vegetation, and low ecological resilience, and the contradiction between large-scale open-pit coal mining and ecological protection is prominent, which directly affects the regional ecological security and the national energy security, and there is an urgent need to carry out the research on the ecological protection and restoration of large-scale open-pit mining areas. The study of ecological protection and restoration of large-scale open-pit mining area is urgently needed. To address the problems of land destruction, dust and sand, falling water level, soil sanding, and vegetation degradation caused by large-scale open-pit coal mining in the northern sand belt, the study is based on the area of the northern sand belt and the size of the open-pit coal mine. Taking the large-scale surface coal mining area in Inner Mongolia, which has the largest area and proportion of surface coal production in the northern sand belt, as the research area, the research idea of “loss-reducing mining - three-dimensional water preservation - soil creation and soil revitalization - systematic restoration - integrated supervision and management” is revealed. Adhering to the research idea of “loss-reducing mining - three-dimensional water conservation - soil creation and living soil - system restoration - integrated supervision”, the research area reveals the transmission mechanism of geotechnical damage and ecological degradation of large-scale surface mines, the mechanism of ecological loss-reducing mining, the mechanism of ecological self-sustainability, and develops key technologies such as loss-reducing mining with ecological protection, ecological design, water resource protection and comprehensive utilization, erosion-resistant geomorphological remodeling, fine reconstruction of the living soil layer in the discharge site, soil improvement, and quality enhancement and capacity increase, and joint restoration of fungus-algae-grass, ecosystem degradation supervision and other key technologies, form an ecological protection and restoration technology system suitable for the open-pit coal mine area in the northern sand-proof belt, and build a large-scale integrated demonstration area for ecological protection and restoration of the open-pit mine area. We will create a low-cost, high-efficiency, sustainable and replicable ecological protection and restoration model for large-scale open-pit coal mining areas, provide support for scientific mining and ecological safety in large-scale open-pit coal mining areas in the northern sand-proof belt of China, and realize the goal of coordinating energy supply and ecological protection in coal development and the goal of “developing the gold and silver mountains and recreating the green mountains and green water”.

In China, groundwater loss caused by underground coal mining is becoming increasingly serious. The key to groundwater restoration is to repair mining-induced water-conducting fractures (WCFs) in the overlying strata. In this study, the adsorption–consolidation sealing characteristics of chemical precipitates were used to conduct permeability reduction (PR) experiments, including adding mixed CaCO3 and Fe(OH)3 to a sandstone specimen with a single fracture at room temperature. An aqueous solution of Na2CO3 was used as the simulated groundwater, and a solution of mixed CaCl2 and FeCl2 was used as the repair reagent to simulate the water seepage conditions of a fractured rock mass. The two aqueous solutions were simultaneously injected into a single-fractured rock specimen at a constant flow rate. The experimental results show that the Fe(OH)3 colloid encapsulated CaCO3 crystals in a mixed precipitate, reducing the overall structural stability of the mixed precipitate and restricting repair and PR efficiency. However, the Fe(OH)3 precipitate had better PR efficiency in the initial stage of the experiment. Therefore, a better scheme was put forward to repair the WCF, utilizing a mixed Fe(OH)3 and CaCO3 precipitate with a molar ratio close to 1:4 in the early stage and a single CaCO3 precipitate in the later stage.

Cytospora canker caused by Cytospora chrysosperma has a wide range of hazards in Xinjiang, seriously affecting the development of Xinjiang’s characteristic forest and fruit industries. Climate change affects the distribution of fungal species and may exacerbate the risk of forest diseases such as cytospora canker. The present study was conducted on C. chrysosperma and makes predictions of potentially suitable area based on 133 records of C. chrysosperma distribution points and 47 environmental factors. We applied the MaxEnt model adjusted by the Kuenm package for feature class parameters (FC) and regularization multipliers (RM) to explore the main environmental factors affecting the geographical distribution of C. chrysosperma. And then we predicted its spatial distribution pattern and centroid change trend in potentially suitable area under three different Representative Concentration Pathways in the current scenario, 2041–2070, and 2071–2,100. The results showed that the optimal model with parameters FC = LQ and RM = 0.3 had the lowest model complexity and overfitting, and the model predicted with very high accuracy, AUC = 0.971 ± 0.0019. Considering the percent contribution, permutation importance of jackknife and single-factor response curve, the main environmental factors affecting its geographical distribution are precipitation seasonality (bio15), aspect cosine (aspectcosine), monthly variability in potential evapotranspiration (PETseasonality), and mean monthly potential evapotranspiration of the coldest quarter (PETColdestQuarter), with a cumulative contribution rate reaching 70.8%. Under the current climate scenarios, the total suitable area for C. chrysosperma is 82.35 × 104 km2, and the highly suitable area is 5.49 × 104 km2, accounting for 6.67% of the total suitable area, primarily located in the Tacheng, Yili, and Changji regions. Meanwhile, centroid transfer analysis indicates a tendency for its distribution to migrate towards lower latitudes under future climatic conditions. The MaxEnt model proposed in this study can be used to predict the distribution and risk of C. chrysosperma in Xinjiang and provide guidance for the prevention and control of cytospora canker.

Under the background of dual-carbon national strategy and the widespread application of new-generation information technologies, systematization, precision, and intellectualization are the important development directions for the governance of mine ecological environment. Informatization of the governance of mine ecological environment in China has been carried out in a relatively rich and long-term way, but there are still problems such as lack of comprehensive monitoring system, inaccurate modeling of ecological evolution, ecological governance relying on expert experience, and no decision-making tools on informatization. Accordingly, in this study, the digital twin of mine ecological environment (DTME) is proposed, and its general framework, functional features and key technologies for system construction are introduced. The DTME is formally defined as a twin system of the physical mine ecosystem in virtual space by mapping three aspects: ecological elements, evolutionary processes, and impact mechanisms. The DTME utilizes spaceborne, airborne, and ground multi-source monitoring devices to dynamically detect the changes of ecological elements in the mine area, and constructs the complex driving models of the ecological evolution based on the artificial intelligence. The DTME supports the whole process of the scientific governance of mine ecological environment through program optimization or feedback control. Furthermore, the general framework of the DTME is introduced, including physical mine ecological environment (physical entity), monitoring terminal, twin data, information link, cloud-edge-terminal service, virtual mine ecological environment (virtual entity), and control terminal. Guided by real-life application requirements, the DTME can achieve mine ecological quality dynamic monitoring and health examination, mine ecological evolution simulation and impact factor analysis, prediction of mine ecological changes, mine ecological risk warning, intelligent grading and zoning of ecological restoration, mine ecological restoration effect evaluation, and mine ecological environment supervision. Finally, it is illustrated that the construction of the digital twin system of mine ecological environment involves four key technologies: digital base of mine ecosystem, digital scene of mine ecosystem, simulation and prediction of mine ecological evolution, and intelligent decision of mine ecological governance. The application of DTME can support the sensing, representation, monitoring, simulation, emulation, prediction, and control of the whole governance process of mine ecological environment, which is promising to significantly improve the quality and efficiency of governance.

Aquifer protection in high-intensity coal mining is difficult to green and safe-mining, due to less knowledge of the response law of local seepage-flow in the mining area after aquifer damaged for the protection method and techniques. Adopting the coupling analysis idea of local multi-field source, the study focuses on the response law of mining-seepage field and aimed to revel coupling relationship between locally mining and the seepage field response and construct local mining groundwater system used to analyze the time-space evolution law of mining-seepage field and its cumulative effects, with the limited hydrological data including ground and underground observation. Based on application analysis of typical case, the analysis method of mining-seepage field is pro-posed, suitable for groundwater protection and engineering restoration in the mining area. The results show: ① Based on coupling relation of “mining excitation-overburden strain-seepage field response” and the energy transfer relationship from mining kinetic and seepage potential energy, the mining-seepage coupling mechanism is proposed. The mining seepage system (MSS) is constructed for describing the state of the operative local seepage field being divided into conduction zone, disturbance zone and radiation zone; ② The simplified mining-seepage effect models are established, including the “columnar seepage model” of the conduction zone and the “well-seepage” state model of the disturbance-radiation zone. Relative analyzing parameters are proposed, such as the miming-seepage coupling coefficient, the mining-seepage flow, the apparent water conductivity, distance of source-measuring point for describing statues of mining-seepage field, and aquifer damage, the risk of water-reserving safe-mining and mining height used for the groundwater protection analysis; ③ Revealed that the mining-seepage coupling coefficient is the key factor affecting the characteristics of the local mining-seepage response, featured that the hard rock overburden is larger than soft rock in coupling strength and influence range, and that the low-conductivity aquifer is narrower than high-conductivity in mining seepage field response area, higher in the response frequency and closer in the cumulative influence distance. Timing variation of mining-seepage flow and the water conductivity are characteristics of the periodicity, amplitude fluctuation and in-rush-flow pulsation of the reactive response; ④ Based on mining-seepage coupling cumulative effect, the identification process of the conduction area is proposed, containing the equilibrium analyzing method for“suppling, run-off, excretion”of mining area and the constraint-equilibrium method for induced-seepage flow of the mining face. Analysis method of overburden thickness, mining risk and mining height proposed, aimed to control mining damage of heterogeneous aquifer; ⑤ The application indicates that the mining-seepage disturbs directly the III aquifer and affects the II aquifer, and the cumulative impact range exceeds respectively 2 km and 6 km, during mining period (2012–2019); that III’ mining-seepage flow is significantly lower than that of II aquifer with obvious synergistic response of mining-seepage, its total amount of the flow close to the actual mine water in the same period; that the initial section W01 mining face is the main conducting zone, with mining-seepage flow sourced from direct“excretion” of III aquifer and indirect supply of II aquifer; that total amount of other mining areas was stable in low-level, mainly from the seepage of III aquifer mining seepage and a small part from the cross-layer replenishment of II aquifer due to periodic subsidence fractures; that strategy of “Protecting II aquifer by Controlling III” and the flexible recovery mode are adopted in the case of large and thick multi-aquifers, in which safe mining height in the western and eastern areas was increased to about 8−12 m and 16 m, respectively, lead to obvious effect on the aquifers protection confirmed by the significantly decreased abnormal level of seepage in the continuation mining area and the phenomenon of no water inrush.

In the published article [...]

The objective phenomenon of seepage reduction and self-healing is easy to occur in the mining fractured rock mass after long-term evolution process of coal seam after mining. In survey of this phenomenon, taking the No. 12401 work face of Bulianta Coal Mine and the No. 52306 work face of Daliuta Coal Mine in Shendong as typical test areas, the engineering exploration and theoretical research on the self-healing law in different areas of overburden were carried out. The evolution law of long-term self-healing process of overburden after mining was revealed. The results show that the fractured overburden of the two coal mines had achieved good self-healing effects 15 years and 7.3 years after mining, respectively. The areas with water conduction within the original water-conducting fractured zone have been significantly reduced. Although the caved zone still conducts water, the height has also been reduced. By taking the ratio of the vertical length of the self-healing section in overburden from the original water diversion to the water isolation to the height of the water-conducting fractured zone as the self-healing recovery rate for statistics, it is obtained that the self-healing recovery rate of No. 12401 work face in the overburden near the mining boundary and in the middle of the dip has reached 59.5% and 53.5% respectively, and the self-healing recovery rate of 52306 work face in the corresponding area is 25.8% and 47.5% respectively. Due to that No. 12401 work face has mined for a longer time and has a larger coal seam burial depth, its self-healing effect of fractured overburden is obviously better. Affected by the occurrence difference of different strata in the vertical direction of the overburden and the initial damasge degree in different areas in the horizontal direction, the distribution of the self-healing area is often discrete and discontinuous, in which the interval of the unrepaired area generally corresponds to the position of the key stratum or thick and hard rock layer, and the shorter the post mining life and the closer to the mining boundary, the more significant the dispersion. The self-healing effect of mining fractured rock mass is actually a process of permeability reduction and evolution under the comprehensive influence of multiple factors. For the mining conditions in Shendong coalfield, the residual settlement caused by stress compaction plays a leading role in the initial process within 1.5 to 2 years after coal mining, which is relatively significant in the overburden in the middle of the mining area. Then, the self-healing process has been dominated by the water-rock or water-gas-rock interactions in goaf. That is, the filling and sealing effect of the derivatives produced in the relevant process on the fractures and the roughness reduction of the fracture surface induced by hydraulic erosion directly affect the self-healing results. Therefore, whether there is long-term water seepage in the overburden after mining determines the difficulty of self-healing of fractured rock mass. Water conducting fractures in the overburden of most mines in Shendong are generally directly connected to the Quaternary loose layer or surface, and in recent years, there is abundant rainfall, which provides superior conditions for the generation of water-rock or water-gas-rock interactions and the promotion of self-healing effect.

Abstract Non-technical summary To address the issues of declining groundwater levels and the degradation of soil ecological functions caused by open-pit coal mining in China. Based on theoretical analysis, laboratory experiments, on-site monitoring, mathematical modeling, and other means, the concept of coal ecological protection mining of ‘damage reduction mining, three-dimensional protection, systematic restoration’ is proposed. The mining concept has achieved remarkable ecological restoration effects, leading the scientific and technological progress of safe, efficient and green mining in open-pit coal mines. Technical summary The mechanism of damage propagation among ‘rock-soil-water’ ecological elements in open-pit coal mining was revealed. Adopting comprehensive damage-reducing mining technology throughout the entire stripping process, mining and drainage, shengli open-pit coal mine has doubled its production capacity, and reduced the land excavation and damage by 60 mu/year, reduced the mining area by 1,128 mu, and raised the groundwater level by 2.6–6 m, and the ecological restoration of the drainage field was advanced by more than 1 year. Adopting the three-dimensional water storage technology involves underground reservoirs, aquifer reconstruction, and near-surface distributed water storage units, baorixile open-pit mine has built the world's first open-pit underground water reservoir, with a water storage capacity of 1.22 million m3, and the speed of groundwater level restoration has been increased by more than 70%. By adopting the systematic restoration technology of geomorphology-soil-vegetation in the discharge site, the soil water content in the demonstration area has been increased by 52%, the survival rate of plants has been increased by 34%, and the vegetation coverage has been increased by more than 40%. Social media summary Damage-reducing mining and systematic ecological restoration in open-pit coal mining are essential for the safe, efficient and green development of coal.

In the long-term evolution process after the development of the water-conducting fractures in overburden, the self-healing phenomenon will occur, in which the water-conducting seepage capacity will gradually decrease. It is of great significance to study the self-healing mechanisms of water-conducting fractures for scientifically guiding the ecological function restoration of groundwater in mining areas. Based on the engineering detection results of self-healing of water-conducting fractures in No. 12401 working face of Bulianta coal mine in 15 years after mining, the mechanisms of repairing and reducing permeability of water-conducting fractures caused by chemical precipitation generated by the mixing of different groundwater from different layers after mining are studied. The results show that the water-conducting fractures in the overburden had directly developed into the Quaternary loose layer, which had connected multiple aquifers in the strata. Due to that the shallow groundwater contains more Ca2+, while the bedrock groundwater contains more CO3 2− and HCO3 −, the CaCO3 precipitation will be produced when the two kinds of groundwater meet and mix in the mining overburden. The sediment migrates with water and is continuously adsorbed on the fracture surface, the scaling process of inclusion co sedimentation consolidation occurs. After a long time of accumulation, the scale or inclusion with certain corrosion resistance is finally formed to plug and repair the fractures. The indoor test shows that the self-healing and permeability reduction effect of the water-conducting fractures caused by this process is stable and rapid. The absolute permeability of the fractured rock specimen is reduced from 0.09×10−15 m2 to 0.0025×10−15 m2 after nearly two months of mixed flow of simulated groundwater with two different water quality, and there is no obvious permeability fluctuation under the condition of 1.5 MPa water pressure. Because the confluence and mixing of different groundwater mainly occur in the fractured rock mass near the mining boundary, the self-healing process of water-conducting fractures and its effect in different areas of overburden will be significantly different. The self-healing of overburden water-conducting fractures in the middle area of No.12401 working face is mainly caused by the water-rock or water-gas-rock interactions caused by rainfall infiltration, while the water-conducting fractures near the mining boundary are dominated by the confluence mixed flow reaction of different groundwater and the water-rock or water-gas-rock interactions, so the self-healing effect of the latter should be obviously better. The field detection results show that after 15 years of self-healing evolution, the water-conducting fractures in the overburden near the mining boundary have been self-repaired in the depth range of 86.7 m to 179.1 m, while the overburden in the middle of the mining area still has local unrepaired phenomena in the corresponding self-healing area, with the depth range of 110.0 m to 118.6 m, which confirms the good repair effect of the chemical precipitation generated by the intersection and mixing of different groundwater.

Abstract The application of drip irrigation has been paid more and more attention, but there was lack of systematic comparative analysis between drip irrigation and conventional border irrigation method for maize, currently. A 7-year field study from 2015 to 2021 evaluated the effects of drip irrigation (DI, 540 mm) or conventional border irrigation method (BI, 720 mm) on maize growth, water use efficiency (WUE) as well as profitability. The results showed the plant height, leaf area index, yield, WUE and economic benefit of maize with DI had significantly higher than BI. The dry matter translocation, the dry matter transfer efficiency and contribution of dry matter translocation to grain with DI showed significant increase of 27.44%, 13.97% and 7.85% compared to BI, respectively. In comparison to conventional border irrigation, the yield of drip irrigation increased by 14.39%, as well as WUE and irrigation water use efficiency (IWUE) increased by 53.77% and 57.89%. The net return and economic benefit of drip irrigation was 1998.87 and 756.58 USD$ hm−1 higher than that of BI. Drip irrigation increased net return and benefit/cost ratio by 60.90% and 22.88% compared with BI. These results demonstrate that the drip irrigation can effectively improve the growth, yield, WUE and economic benefit of maize in northwest China. Therefore, drip irrigation can be used for maize cultivation to increase crop yield and WUE in northwest China, which has cut down on irrigation water about 180 mm.

Abstract Dust pollution is one of the most severe environmental issues in open pit mines, hindering green mining development. Open pit mine dust has characteristics of multiple dust-generating points, is irregular, influenced by climatic conditions, and has a high degree of distribution with a wide dispersion range in three dimensions. Consequently, evaluating the quantity of dust dispersion and controlling environmental pollution are crucial for supporting green mining. In this paper, dust monitoring above the open pit mine was carried out with an unmanned aerial vehicle (UAV) on board. The dust distribution patterns above the open pit mine were studied in different vertical and horizontal directions at different heights. The results show that the temperature changes less in the morning and more at noon in winter. At the same time, the isothermal layer becomes thinner and thinner as the temperature rises, which makes it easy for dust to spread. The horizontal dust is mainly concentrated at 1300 and 1550 elevations. The dust concentration is polarized at 1350–1450 elevation. The most serious exceedance is at 1400 elevation, with TSP (the concentration of total suspended particulate), PM10 (particulates with aerodynamic diameter

Mulching is a widely used agricultural water conservation measure in the semiarid regions of Northwest China. In order to explore the response process of different film mulching methods to soil microorganisms, we characterized the effect of different film mulching methods on soil microbial diversity and community structure characteristics in the root zone of drip-irrigated maize during the heading and maturity stages using high-throughput sequencing of 16SrDNA and ITS amplicons combined with bioinformatics analysis. Full mulching (FM) was contrasted to controls of no mulching (NM) and half-mulching (HM), yielding an order of microbial diversity, abundance, and evenness scores of HM > FM > NM. The HM and FM treatments reduced the relative abundance of Proteobacteria and Actinobacteria (the most abundant bacteria) in the bacterial community structure but increased that of Acidobacteria and Chloroflexi. In the fungal community structure, HM decreased the abundance of Sordariomycetes but increased that of Eurotiomycetes (the most abundant fungi). The abundance and community structure of bacteria were significantly correlated with soil temperature and those of fungi with pH. HM improved network complexity and competitive relationships among bacteria, while FM increased the relationship between fungal groups and the symbiosis of fungal communities. HM significantly increased maize yield (20.37% and 6.01% above NM and FM, respectively). In summary, full mulching was more favorable than no mulching for soil microbial diversity and community structure composition, but soil microbial diversity and yield responded better to half-mulching. These results provide a background for improving the yield of drip-irrigated maize and protecting the microbial ecosystems of farmland soils.

Abstract Starting from the source of mining, scientific understanding of surface damage law and assessment method in longwall mining of shallow coal seam is conducive to solving the problems of geological hazards and deterioration of the ecological environment, and promoting the coordinated development of efficient coal mining and environmental protection. Based on numerical simulation and theoretical analysis, the surface damage process and spatiotemporal evolution of fracture field are discussed. The influencing factors and assessment method of surface damage are clarified. The results show that surface damage undergone the immediate roof caving stage, the fracture and instability stage of main roof, the spatial amplification stage of separation layer, the instability stage of surface damage control layer and the mining damage stability stage. Under the critical extraction condition, the cracks above the goaf are divided into the crack area outside the cut, the crack area inside the cut, the re-compaction area in the middle goaf, the crack area behind the longwall face, and the crack area in front of the longwall face. The overburden reaches critical failure ahead of surface critical mining. The sensitivity of loose layer thickness to surface subsidence coefficient is greater than that of mining thickness to surface subsidence coefficient. Surface damage control should be adjusted to local conditions, and finally realize zoning treatment and zoning repair. Through the three-step method of "longwall face rapid advancing method, local grouting reinforcement overburden method and zoning treatment ground fissures method", the surface damage control of 12,401 longwall face is realized. This research provides theoretical guidance and application value for surface ecological restoration in similar mining area.

Rotundic acid (RA), an ursane-type pentacyclic triterpene acid isolated from the dried barks of Ilex rotunda Thunb. (Aquifoliaceae), possesses diverse bioactivities. To further study its pharmacokinetics, a simple and sensitive liquid chromatography with triple quadrupole mass spectrometry (LC-QqQ-MS/MS) method was developed and validated to quantify RA concentration in rat plasma and tissue using etofesalamide as an internal standard (IS). Plasma and tissue samples were subjected to one-step protein precipitation. Chromatographic separation was achieved on a ZORBAX Eclipse XDB-C18 column (4.6 mm × 50 mm, 5 μm) under gradient conditions with eluents of methanol:acetonitrile (1:1, V/V) and 5 mM ammonium formate:methanol (9:1, V/V) at 0.5 mL/min. Multiple reaction monitoring transitions were performed at m/z 487.30 → 437.30 for RA and m/z 256.10 → 227.10 for IS in the negative mode. The developed LC-QqQ-MS/MS method exhibited good linearity (2–500 ng/mL) and was fully validated in accordance with U.S. Food and Drug Administration bioanalytical guidelines. Dose proportionality and bioavailability in rats were determined by comparing pharmacokinetic data after single oral (10, 20, and 40 mg/kg) and intravenous (10 mg/kg) administration of RA. Tissue distribution was studied following oral administration at 20 mg/kg. The results showed that the absolute bioavailability of RA after administration at different doses ranged from 16.1% to 19.4%. RA showed good dose proportionality over a dose range of 10–40 mg/kg. RA was rapidly absorbed in a dose-dependent manner and highly distributed in the liver. In conclusion, this study is the first to systematically elucidate the absorption and distribution characteristics of RA in rats, which can provide additional information for further development and evaluation of RA in drug metabolism and pharmacokinetic studies.

The role of coal mine underground reservoirs (CMUR) in ‘guide storage and use’ greatly solves the problem of mine water waste. Replenishment and prediction of reservoir water sources provide an important support for the successful development of key technologies concerning CMUR. To study water replenishment for CMUR, the hydrogeological conditions of the Shendong mining area were investigated as an example. Based on the relationship between the development height of the ‘two belts’ of shallow coal seams and the relative occurrence of location of aquifers, the aquifers are generalized and classified according to occurrences location. Taking Bulianta coal mine as the research background, a discrete element fluid–solid coupling numerical simulation model is constructed to analyze the development characteristics of mining-induced fractures after coal seam mining, and the water replenishment channel of the CMUR is determined. On this basis, analysis is made on the change law of water pressure in the aquifer, aquifuge and coal seam roof under mining action. Taking hydraulic head pressure and water velocity as the judgment basis, prediction and evaluation are made on the water replenishment capacity of CMUR. The research results can provide references for coal mine safety production and mine water protection and utilization. HIGHLIGHTS Based on the water-conducting fractured zone, the occurrence type of ‘coal-water’ is generalized.; The change law of aquifer under different ‘coal-water’ conditions is revealed.; The source and amount of water supply for CMUR were obtained under different conditions.;

In this paper, the open-pit coal mine in semi-arid grassland was taken as the research object. The water samples of the open-pit coal mine and its surrounding areas were collected and the hydrochemical parameters were detected. The temporal and spatial distribution characteristics of the parameters such as pH, electrical conductivity, and dissolved oxygen in the groundwater were studied. The results showed that the groundwater in the study area was alkaline and brackish water. Climate factors might have a certain impact on the pH, conductivity, and dissolved oxygen of groundwater. The pH value of groundwater in the mining area was higher than that in the surrounding pastoral area, while the conductivity value of the mining area was between the pastoral area in the west and the Xilin river area in the east. The parameters of pH, conductivity and total dissolved solids of the four monitoring wells around the mining area showed a slow change or stable phenomenon in the vertical direction with the increase of groundwater depth. This study is of great significance to understand the characteristics of groundwater chemistry in mining areas and the rational development and utilization of groundwater resources.

The soil in Yuncheng Salt Lake has serious salinization and the biogeographic environment affects the composition and distribution of special halophilic and salt-tolerant microbial communities in this area. Therefore, this study collected soils at distances of 15, 30, and 45 m from the Salt Lake and used non-saline soil (60 m) as a control to explore the microbial composition and salt tolerance mechanisms using metagenomics technology. The results showed that the dominant species and abundance of salt-tolerant microorganisms changed gradually with distance from Salt Lake. The salt-tolerant microorganisms can increase the expression of the Na+/H+ antiporter by upregulating the Na+/H+ antiporter subunit mnhA-G to respond to salt stress, simultaneously upregulating the genes in the betaine/proline transport system to promote the conversion of choline into betaine, while also upregulating the trehalose/maltose transport system encode genes to promote the synthesis of trehalose to resist a high salt environment.

Water supply prediction and control is one of the key issues in exploitation of coal mine underground reservoirs (CMUR). In order to investigate the water supply for underground reservoirs in coal mines, the aquifers were classified into three types (types I, II, and III) according to the relative location of aquifers. The discrete element fluid structure coupling numerical simulation model is constructed according to the classification results. The numerical simulation parameters are inversed based on the surface subsidence data and the advance support pressure, and then, the disturbed characteristics and water pressure variation law of different types of aquifers are studied. The research results show that the maximum water inflow of type III, type II, and type I aquifers is 739 m3/h, 377 m3/h, and 279 m3/h, respectively. The dynamic calculation process of underground reservoir capacity under mining disturbance is refined, and the regulation and storage methods of underground reservoir are preliminarily put forward.

Groundwater distribution influenced by mining activities is hard to be tracked due to the lack of accurate and real-time monitoring data. To address the groundwater dynamic change data under the mining conditions of open-pit coal mines, a three-dimensional automatic observation network of groundwater was constructed, and this observation network takes open-pit as the center, showing a radioactive distribution, based on hydrogeological conditions, and observes different aquifers. The data was analyzed by integrating hydrology, mining activity, and meteorological data. Analytic hierarchy process was adopted to reveal the underground disturbance influencing factors, and suggestions for groundwater protection were given. The results show that the dynamic variations of the groundwater with time can be divided into 3 types (precipitation affected, unaffected type, and mining affected types). The drainage operation during the open-pit mining triggered the drop of groundwater level, resulting in a cone of depression. The maximum drawdown of the central groundwater level was 60 m. And the disturbance mainly occurred in the mining area where the maximum groundwater disturbance radius was 8 km. Artificial drainage was the main disturbance factor of groundwater, and the range of groundwater level drop within the disturbance radius was closely related to the distance from the open-pit coal mine and the drainage volume. The closer the distance to the mines and the higher the drainage volume led to the deeper the groundwater level drop. This study shows the importance of the three-dimensional observation network of groundwater and provides a good reference for groundwater resources protection and ecological restoration in open-pit coal mines.

To explore the influence law and sensitivity of various factors on the height of the water-conducting fracture zone, and further predict the development height of the water-conducting fracture zone, taking the field survey and collected actual measured data as the research foundation, establishing mathematical model, regression analysis, and field measurement were adopted comprehensively. Based on the Data Processing System (DPS), influence factors of the development height of the water-conducting fracture zone were analyzed. By introducing sensitivity coefficient, a sensitivity quantification mathematical model was established, and sensitivity ranking was obtained. The regression relationship between each factor and the height of the water-conducting fracture zone was obtained by single factor analysis. Based on this, the prediction formula was proposed and successfully used in the field practice. Results show that the sensitivity coefficient of mining thickness, ratio coefficient of hard rock lithology, mining depth, and length of working face was 0.75, 0.69, 0.66, and 0.58, respectively. The dispersion degree of working face length and mining depth was greater than that of ratio coefficient of hard rock lithology and mining thickness. To some extent, it also reflects that the sensitivity of working face length and mining depth to the height of the water-conducting fracture zone was the weakest, which was consistent with the previous conclusions. Using the multiple regression mathematical model, a nonlinear statistical relationship between the height of the water-conducting fracture zone and ratio coefficient of hard rock lithology, mining thickness, working face length, and mining depth was obtained. This research provides some scientific basis and guidance for safe and efficient underwater mining.

Longwall mechanized top coal caving mining (LMTCCM) in extra-thick coal seams has its own characteristics. The law of mining pressure and overlying strata failure height in extra-thick coal seams are much larger than those of medium-thick and thick coal seams. The key stratum structure morphology also has an important influence on the law of overlying strata movement and stability of surrounding rock. Based on the engineering geological conditions, this paper used the method of theoretical analysis and numerical simulation to study the key stratum structure morphology of LMTCCM in extra-thick coal seams. The results show that under the condition of LMTCCM in extra-thick coal seams, the key stratum forms the structure of low cantilever beam and high hinged rock beam. With the increase of coal seam thickness, the breaking position of cantilever beam is closer to the coal wall. Through theoretical calculation, it is obtained that the breaking length of cantilever beam is 31.5 m and the breaking position of cantilever beam is 15.4 m away from coal wall. With the increase of cycle, key strata will undergo the evolution law from the generation of longitudinal cracks to the hinged structure and then to the cantilever beam structure. The breakage of key strata will cause the expansion of longitudinal cracks and the overall synchronous movement of overlying strata. With the increase of coal seam thickness, the distribution of longitudinal cracks will gradually transfer from the upper part of goaf to the deep part of coal body in space and increase in quantity. This research is of great significance for improving the stability of overlying strata and ensuring the safe and efficient mining of extra-thick coal seams.

Quite a number of shallow buried coal seams (SBCS) are distributed in China. The overburden is easily damaged due to the mining of SBCS, resulting in water resources loss and surface damage. Taking 12403 working face of Wulanmulun coal mine in Shendong mining area as an example, this paper analyzed the overburden migration and failure characteristics after mining SBCS with thick loose layer based on actual measurement data and simulation results. The results show that the subsidence of strata in caving zone has no skewness feature along strike direction, while the subsidence of strata in fracture zone and bending subsidence zone shows skewness subsidence phenomenon. An interface exists in the overburden, and the movements of upper and lower strata at the interface have different characteristics. The cracks penetrating the whole strata exist in bedrock, not in aeolian sand. The height of water flowing fracture zone is 35.74 m–62.89 m according to the loss of fluid in the borehole and consistent with the results of numerical simulation and similar simulation. This study can provide a reference for the prediction of the height of water flowing fracture zone and the overburden migration in mining SBCS.

This study experimentally investigated the self-healing behavior, referring to the naturally occurring water permeability decrease, of fractured rocks exposed to water-CO2-rock interaction (WCRI). The experiment was conducted on prefractured specimens of three rock types typical of the Shendong coalfield: coarse-grained sandrock, fine-grained sandrock, and sandy mudrock. During the experiment, which ran for nearly 15 months, all three specimens exhibited decreasing permeabilities. The coarse- and fine-grained sandrock specimens exhibited smooth decreases in permeability, with approximately parallel permeability time curves, whereas that of the sandy mudrock specimen decreased rapidly during the initial stage and slowly during later stages. The sandrock specimens were rich in feldspars, which were dissolved and/or corroded and involved in ionic exchange reactions with CO2 and groundwater, thereby generating secondary minerals (such as kaolinite, quartz, and sericite) or CaSO4 sediments. These derivative matters adhered to the fracture surface, thereby gradually repairing fractures and decreasing the water permeability of the fractured rocks. In comparison, the sandy mudrock had a high content of clay minerals, and the water-rock interaction caused rapid expansions of illite, mixed illite-smectite, and other clay minerals, thereby narrowing the fractures and causing the rapid permeability decrease during the initial stage. In later stages, the derivative matters generated by the dissolution and/or corrosion of feldspars and other aluminum silicate minerals in the mudrock filled and sealed the fractures, causing the slow permeability decreases during the later stages, as in the sandrock specimens. Neutral and basic groundwater conditions facilitated better self-healing of fractured mudrocks rich in clay minerals, whereas acidic groundwater conditions and the presence of CO2 facilitated better self-healing of fractured sandrocks. Thus, this study’s results are of significant value to aquifer restoration efforts in the Shendong coalfield and other ecologically vulnerable mining areas.

This study conducted land cover classification and inversion analysis to estimate land surface temperature, soil moisture, specific humidity, atmospheric water vapor density, and relative humidity using remote sensing and multi-source mining data. Using 1990–2020 data from the Shendong mining area in Inner Mongolia, China, the eco-environmental evolution and the ecological cumulative effects (ECE) of mining operations were characterized and analyzed at a long-term scale. The results show that while the eco-environment was generally stable, mining activities affected the eco-environment at the initial stage (1990–2000) to a certain degree. During the rapid development stage of coal mining, the eco-environment was severely damaged, and the ECE were significant at the temporal scale. The absolute value of the change rate of ecological parameters was increasing. Due to an increased focus on ecological restoration, starting in 2010, the environmental indicators gradually stabilized and the eco-environment improved considerably, ushering in a period of stability for coal mining activities. The absolute value of the change rate of ecological parameters became stable. Analysis of the change in eco-environmental indicators with distance and comparison to the contrast area showed the ECE characteristics from mining disturbance at the spatial scale. This study shows that remote sensing technology can be used to characterize the ECE from mining operations and analyze eco-environmental indicators, providing crucial information in support of ecological protection and restoration, particularly in coal mining areas.

The gold nanoclusters (Au-NCs) have emerged for delivery of various payloads such as small molecular drug and big molecular proteins due to their low immunogenicity, quick clearance and good biocompatibility. Even though they have shown great prospective in biomedical area, there are also some security problems which should be investigated further more. BRCAA1 mAb Au-NCs is a probe for gastric cancer diagnosis prepared by conjugating BRCAA1 monoclonal antibody with fluorescent Au-NCs. In this research, the renal excretion and effect on glomerulus filtration of BRCAA1 mAb AuNCs in rats were investigated. The result indicated that BRCAA1 mAb Au-NCs could be eliminated through the glomerulus filtration as early as 2 hr after the administration following the excretion of other proteins in the plasma such as immunoglobulin. Electron microscope image and pathological analysis of kidney showed that BRCAA1 mAb Au-NCs induced some structural injury such as capillaries endotheliocyte damage, pycnosis, karyorrhexis and hyalomitome acidophilia enhancement. Biochemical indexes including ALT, AST, BUN, TBIL, CK and CREA are abnormal in the period of 2 to 48 hr after the administration. The results of Western blotting and immunohistochemisty show the downregulation of three key proteins (ZO-1, Nephrin and Podocin) indicating the influence of BRCAA1 mAb Au-NCs on the foot processes of glomerular epithelial cells and shallow intercellular junctions or slit diaphragms.

Microseismic monitoring technology was used to study the real-time evolution of rock mass damage generated by a working face as it approached a fault in Dongjiahe Coal Mine. The influence of vertical zoning of overlying strata on damage at the fault was analyzed. Numerical simulation using finite element method based on meso-statistical damage theory was used to investigate the nonlinear and nonuniform failure behaviour of the rock mass near the fault. The response of the fault stress to excavation activity and the rule of fault activation were examined. The results show that the fault damage has segmental characteristics. Microcracks are first generated at the fractured zone that is divided into lower, middle, and upper sections, located 30∼70 m, 120∼180 m, and 230∼280 m above the coal seam, respectively. There was also a segmentation phenomenon in the stress response of fault. The risk of fault activation was evaluated by using the ratio of shear stress to the maximum principal stress. When the working face was 260 m and 140 m away from the fault, the activation risk at the upper-middle and lower sections began to increase, respectively. When the fault was within 60 m, the risk of fault activation was highest.

Deoxyglycychloxazol (TY501) is a glycyrrhetinic acid derivative which exhibits high anti-inflammatory activity and reduced pseudoaldosteronism compared to glycyrrhetinic acid. In this study, a sensitive and rapid liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was established for the quantitation of TY501 in rat plasma. Plasma samples were treated by precipitating protein with methanol and supernatants were separated by a Symmetry C8 column with the mobile phase consisting of methanol and 10 mM ammonium formate (containing 0.1% of formic acid) (90:10, v/v). The selected reaction monitoring (SRM) transitions were performed at m/z 647.4→191.2 for TY501 and m/z 473.3→143.3 for astragaloside aglycone (IS) in the positive ion mode with atmospheric pressure chemical ionization (APCI) source. Calibration curve was linear over the concentration range of 5–5000 ng/mL. The lower limit of quantification was 5 ng/mL. The mean recovery was over 88%. The intra- and inter-day precisions were lower than 6.0% and 12.8%, respectively, and the accuracy was within ±1.3%. TY501 was stable under usual storage conditions and handling procedure. The validated method has been successfully applied to a pharmacokinetic study after oral administration of TY501 to rats at a dosage of 10 mg/kg.

The evolution and distribution of fracture network induced by mining is essential to determine the mechanical properties and permeability of disturbed rock mass. In this paper, the similar material model tests are employed to simulate the stress variation, cyclic breaking, and fracture formation and distribution status of the overlying strata with different loading conditions, rock properties, and mining process. The fractal dimension of mining-induced fracture network varied with mining advancing, and the evolvement laws of fracture network with mining advancing and different mining advancing footage are concerned and obtained. By establishing the relationship between the fractal dimension and the mining length in different horizontal and vertical zones, it demonstrates that the fractal dimensions in horizontal and vertical zones have a self-similar characteristic, and the distribution of the fractal dimension of the mining-induced fractures shows generally the “W”-type trend.

Phosphorus (P) is the main limiting nutrient in agriculture. Most of the P fertilizers applied to the soil remain ineffective because of the weak movement and transfer of P in the soil. Current agricultural production requires large amounts of P fertilizers, leading to the accumulation of residual P in the soil and serious ecological problems. Fixation of P is a vital reason for limited fertilizer effectiveness. Therefore, improving soil P effectiveness is the key to reducing soil P accumulation. This study explored the effects of different water-soluble P fertilizers on maize yield and economic benefits by comparing the relationships between soil P distribution and P absorption and utilization of maize in calcareous soil of Xinjiang province in China. The main results indicated that (1) the drip application of ammonium polyphosphate (APP) and urea phosphate (UP) was more beneficial for increasing the total P content in the deep soil layer; the drip application of APP was beneficial in increasing the available P content of the deep soil layer. (2) Dripping acid fertilizer was more conducive to reducing soil acidity and alkalinity (pH). (3) The application of different P fertilizers favoured the accumulation of P in all organs of maize, among which APP and UP were the most effective since they increased accumulation by 75.8% and 60.4% (at the flowering stage) and 98.2% and 62.3% (at the maturity stage), respectively, compared with CK (without P fertilizer). (4) pH was the main factor affecting P uptake and transformation, and the drip application of P fertilizer had the most significant effect on pH. (5) The application of different water-soluble P fertilizers increased maize yield and economic benefits. Among the five water-soluble P fertilizers selected in this study, APP had the greatest economic benefits, followed by UP and monoammonium phosphate (MAP); they increased yields by 31.6%, 24.5%, and 22.0%, respectively, compared with CK. [ABSTRACT FROM AUTHOR]

Mechanical softening behaviors of shale in CO2-water–rock interaction are critical for shale gas exploitation and CO2 sequestration. This work investigated the cross-scale mechanical softening of shale triggered by CO2-water–rock interaction. Initially, the mechanical softening of shale following 30 d of exposure to CO2 and water was assessed at the rock-forming mineral scale using nanoindentation. The mechanical alterations of rock-forming minerals, including quartz, muscovite, chlorite, and kaolinite, were analyzed and compared. Subsequently, an accurate grain-based modeling (AGBM) was proposed to upscale the nanoindentation results. Numerical models were generated based on the real microstructure of shale derived from TESCAN integrated minerals analyzer (TIMA) digital images. Mechanical parameters of shale minerals determined by nanoindentation served as input material properties for AGBMs. Finally, numerical simulations of uniaxial compression tests were conducted to investigate the impact of mineral softening on the macroscopic Young’s modulus and uniaxial compressive strength (UCS) of shale. The results present direct evidence of shale mineral softening during CO2-water–rock interaction and explore its influence on the upscale mechanical properties of shale. This paper offers a microscopic perspective for comprehending CO2-water-shale interactions and contributes to the development of a cross-scale mechanical model for shale.

Abstract Pest rodents persistently undermine crop yields and food security. Fertility control could be a viable alternative for managing rodent populations. This study investigates the antifertility effects of various concentrations of clarithromycin combined with 1.0 mg/kg quinestrol on male rodents to determine an effective contraceptive dose that minimizes quinestrol usage, addressing key concerns such as potential environmental residue, which may impact ecological balance, and poor palatability, which could reduce ingestion and limit the sterilant’s effectiveness. Male mice were divided into five groups and administered different doses of clarithromycin or clarithromycin and quinestrol for three consecutive days, while the control group received sunflower seed oil only. After seven days, organ weights, reproductive organ weights, sperm density, serum hormone levels, and CYP3A4 content in small intestinal and liver tissues were measured to assess persistent effects. Compared with the control group, all treatment groups had significant reductions in epididymal weight, seminal vesicle weight, and serum T and LH levels. Higher concentrations of clarithromycin (2 mg/kg and 10 mg/kg) significantly impacted reproductive metrics, including sperm density, organ weights, and serum LH and testosterone levels, though complete sterilization was not achieved, with more than 60 million cauda epididymal spermatozoa remaining. However, the combination demonstrated potential as an effective strategy for male fertility control. The combination of 2.0 mg/kg clarithromycin and quinestrol can mitigate organ enlargement seen with quinestrol alone. This combination also decreased total enzyme content, thereby diminishing quinestrol’s induction of CYP3A4, which may increase the sterilization effectiveness of the treatment.

Through the innovative combination of CO2 capture in the coal chemical industry and oil reservoir CO2 storage, Yanchang Petroleum has realized multiple benefits such as clean utilization of coal, carbon emission reduction, and crude oil production increase, and explored a new path for low-carbon development of coal chemical industry. The whole process integration technology and field practice of carbon capture, utilization, and storage (CCUS) from Yanchang Petroleum are systematically presented. For CO2 capture, the low-temperature methanol washing and low-cost CO2 capture technology of the coal chemical industry has been formed. The CO2 capture cost is 105 yuan/t, and the capture demonstration scale is 300000 t/a. For CO2 storage, the storage site selection and potential evaluation methods are established. The evaluation results showed that the theoretical CO2 storage capacity of the oilfield is 8.84×108 t, and the suitable storage area is mainly distributed in the west of the oilfield. In addition, the evolution laws of CO2 storage state across the time scale of storage are revealed. Under the water-gas alternation scenario, the proportions of CO2 storage capacity for structure, residual, solubility and mineral trapping corresponding to 10 years and 100 years is 24.38%, 27.19%, 48.38%, and 0.05%; and 15.09%, 38.65%, 45.77% and 0.49%, respectively. Then the sealing evaluation methods of the caprocks are created. It is specified that the Chang 4+5 cap layer in the Huaziping CO2 sequestration test area mainly belongs to type Ⅰ and type Ⅱ. Finally, a “Trinity” CO2 safety monitoring system is established. The results of safety monitoring show that, there is no CO2 geological leakage in the test area and the safety level of CO2 storage is Ⅰ. The safe and effective storage capacity of CO2 is 10.12×104 t in the field application. The technology of CO2 capture in the coal chemical industry and reservoir CO2 storage has achieved good effects of carbon reduction and crude oil production increase in field practice.