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4 results on '"Qualité des Produits Animaux Equipe Typicité Aromatique et Authentification (INRA)"'

1. Characterization of arabinogalactan-rich mucilage from Cereus triangularis cladodes

Author

E. Engel, Redouan Elboutachfaiti, Cédric Delattre, Laurent Poughon, Benjamin Petera, Guillaume Pierre, Philippe Michaud, Taratra Andrée Fenoradosoa, Anne Wadouachi, Faculté des Sciences, Université d'Antsiranana, B.P. 0, Antsiranana 201, Université d'Antsiranana (UNA), Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Glycochimie, des Antimicrobiens et des Agro-ressources - UMR CNRS 7378 (LG2A ), Université de Picardie Jules Verne (UPJV)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Glucides (LG), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Picardie Jules Verne (UPJV), Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), French government research program Investissements d'avenir' through IMobS3 Laboratory of Excellence, ANR-10-LABX-16-01, European Union through program Regional competitiveness and employment (ERDF-Auvergne region), Auvergne Region, Institut Pascal - Clermont Auvergne (IP), Sigma CLERMONT (Sigma CLERMONT)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Glycochimie, des antimicrobiens et des agro-ressources (LG2A ), Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Glucides (FRE 3517), Centre National de la Recherche Scientifique (CNRS), and Qualité des Produits Animaux Equipe Typicité Aromatique et Authentification (INRA)

Subjects
Cactaceae, Antioxidant, Polymers and Plastics, Arabinogalactan, Mucilage, medicine.medical_treatment, Biology, Polysaccharide, Galactans, Antioxidants, chemistry.chemical_compound, Materials Chemistry, medicine, Cladodes, Madagascar, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Food science, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], chemistry.chemical_classification, Plant Extracts, Organic Chemistry, Galactan, biology.organism_classification, 3. Good health, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], chemistry, Cereus, Biochemistry, Hydroxyl radical
Abstract

International audience; Cereus triangularis (Cactaceae) is a cactus used in food decoction as a traditional medicine in the North region of Madagascar to reduce stomach ache and intestinal diseases. Hydrocolloids were sequentially extracted from its cladodes with a yield of 24% (240 mg/g based on dried cladodes powder). Structural analyses has revealed that this polysaccharide with a molecular mass of 8430,000 g/mol was mainly composed of a galactan backbone of a (1 → 4) linked β-d-Galp residues probably substituted at position 3 by l-arabinofuranosyl residues. In vitro antioxidant activity of this arabinogalactan-rich fraction was detected and quantified by radical DPPH scavenging, hydroxyl radical scavenging, radical anion superoxide scavenging and reducing power method.

Published
2015
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2. The origin of Listeria monocytogenes 4b isolates is signified by subproteomic profiling

Author

Bruno Meunier, Mickaël Desvaux, Jean-Louis Berdagué, Christophe Chambon, Michel Hébraud, Emilie Dumas, Microbiologie Equipe Qualité et Sécurité des Aliments (INRA), Institut National de la Recherche Agronomique (INRA), Unité de Recherches sur les Herbivores (URH), Qualité des Produits Animaux Equipe Typicité Aromatique et Authentification (INRA), Plateforme Proteomique (INRA), Herbivores Equipe Croissance et Métabolisme du Muscle (INRA), SECurité des ALIments et Microbiologie, Institut National de la Recherche Agronomique (INRA)-École nationale d'ingénieurs des techniques des industries agricoles et alimentaires (ENITIAA)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Unité de Nutrition Humaine (UNH), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), and Unité de Microbiologie (MIC)

Subjects
Serotype, Proteomics, Proteome, Biophysics, Protein Array Analysis, Gene Expression, Extracellular proteome, Biology, medicine.disease_cause, Biochemistry, Peptide Mapping, Analytical Chemistry, Microbiology, 03 medical and health sciences, Listeria monocytogenes, Bacterial Proteins, medicine, Food microbiology, Humans, Cytoplasmic proteome, Electrophoresis, Gel, Two-Dimensional, Listeriosis, Serotyping, Peptide-mass fingerprint, Molecular Biology, Gene, 030304 developmental biology, Gel electrophoresis, 0303 health sciences, 030306 microbiology, MALDI-TOF mass spectrometry, Biodiversity, Serovar 4b, Genes, Bacterial, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Food Microbiology, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Abstract

International audience; Among the 13 known serovars of Listeria monocytogenes, strains exhibiting the serovar 4b are the most prevalently involved in epidemics of human listeriosis. The molecular reasons for the major involvement of serovar 4b strains in all major foodborne outbreaks in contrast to the lower prevalence of this serovar among food isolates remain indefinite. In order to provide further insight in the protein expression of L. monocytogenes 4b strains, the cytoplasmic and extracellular proteomes of L. monocytogenes 4b strains from different origins, i.e. environmental, clinical and asymptomatical carriage, were investigated by two-dimensional gel electrophoresis. Statistical hierarchical clustering analysis on subproteomic profiles clearly discriminated the strains according to their origin. Protein spots differentiating the subproteome patterns were identified using MALDI-TOF MS through their peptide mass fingerprint.

Published
2009
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3. Comparative analysis of extracellular and intracellular proteomes of Listeria monocytogenes strains reveals a correlation between protein expression and serovar

Author

Bruno Meunier, Christophe Chambon, Mickaël Desvaux, Emilie Dumas, Michel Hébraud, Jean-Louis Berdagué, Microbiologie Equipe Qualité et Sécurité des Aliments (INRA), Institut National de la Recherche Agronomique (INRA), Herbivores Equipe Croissance et Métabolisme du Muscle (INRA), Qualité des Produits Animaux Equipe Typicité Aromatique et Authentification (INRA), Plateforme Proteomique (INRA), Unité de Recherches sur les Herbivores (URH), SECurité des ALIments et Microbiologie, Institut National de la Recherche Agronomique (INRA)-École nationale d'ingénieurs des techniques des industries agricoles et alimentaires (ENITIAA)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), and Unité de Microbiologie (MIC)

Subjects
Serotype, Proteome, Extracellular, Virulence, Biology, medicine.disease_cause, Peptide Mapping, Applied Microbiology and Biotechnology, Microbiology, 03 medical and health sciences, Bacterial Proteins, Listeria monocytogenes, Peptide mass fingerprinting, medicine, Cluster Analysis, Electrophoresis, Gel, Two-Dimensional, 030304 developmental biology, Serine protease, Gel electrophoresis, 0303 health sciences, Ecology, Bacteria, 030306 microbiology, Protein, Intracellular, Correlation, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Food Microbiology, biology.protein, Comparative study, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Food Science, Biotechnology
Abstract

Listeria monocytogenes , the etiologic agent of listeriosis, remains a serious public health concern, with its frequent occurrence in food environments coupled with a high mortality rate. Among the 13 serovars, human listeriosis is mostly associated with the serovar 4b, 1/2b, and 1/2a strains. To investigate the diversity of L. monocytogenes , the intracellular and extracellular proteins of 12 strains were analyzed by two-dimensional gel electrophoresis. These strains had different origins, belonged to different serovars (4b, 1/2a, and 1/2b), and presented with different levels of virulence in chicken embryos. The clustering of the strains in two groups based on proteomic patterns is in agreement with the L. monocytogenes phylogenetic lineages. Statistical analysis did not allow for identification of proteins specific to the isolate origin or the virulence level of the strains, but 26 and 21 protein spots were shown to be significantly overexpressed and underexpressed, respectively, in the six strains of serovar 1/2a (lineage II) compared to strains of serovar 1/2b or 4b. Moreover, a penicillin-binding protein was specific for serovar 1/2b and two protein spots identified as a serine protease were specific to serovar 4b. These protein spots, identified through peptide mass fingerprinting using matrix-assisted laser desorption ionization-time of flight mass spectrometry, were essentially found in the extracellular proteome and may have uses as potential markers for serotyping and risk analysis.

Published
2008
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4. Optical fiber-based synchronous fluorescence spectroscopy for bacterial discrimination directly from colonies on agar plates.

Author

Belal T, Romdhane K, Jean-Louis B, Tahar B, Eric D, and Françoise L

Abstract

The development of experimental conditions for rapid bacterial discrimination using fluorescence spectroscopy fingerprinting is presented. Colonies of Pseudomonas and related reference strains on agar plates were analyzed directly using an optic fiber coupled to a laboratory spectrofluorimeter. Spectra were collected using either classic fluorescence spectroscopy after excitation at 250 nm and 340 nm for aromatic amino acids and nucleic acids (AAA + NA) and nicotinamide adenine dinucleotide (NADH) respectively, or synchronous scanning in the excitation wavelength range 250-500 nm. Factorial discriminant analysis (FDA) showed 100% correct classification at the genus and species level from NADH spectra and 100% correct classification at the genus and species level for λ = 30, 70, 90 and 110 nm (cross-validation). Analysis of variance (ANOVA) confirmed that culture time (48 or 72 h) colony and optic fiber positioning had non-significant impacts on differences between species. The use of optical fiber-fluorescence spectroscopy for bacterial discrimination directly on colonies is fast, simple and reliable. The results are independent of culture growth phase and neither need reagent addition nor prior manual preparation of cells, thus eliminating all risk of human error or contamination during sample work-up.

Published
2011
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