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35 results on '"Quagliarini, Erica"'

4. Multifunctional FFP2 Face Mask for White Light Disinfection and Pathogens Detection using Hybrid Nanostructures and Optical Metasurfaces

Author

Zaccagnini, Federica, primary, De Biase, Daniela, additional, Bovieri, Francesca, additional, Perotto, Giovanni, additional, Quagliarini, Erica, additional, Bavasso, Irene, additional, Mangino, Giorgio, additional, Iuliano, Marco, additional, Calogero, Antonella, additional, Romeo, Giovanna, additional, Singh, Dharmendra Pratap, additional, Pierini, Filippo, additional, Caracciolo, Giulio, additional, Petronella, Francesca, additional, and De Sio, Luciano, additional

Published
2024
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10. Mechanistic Insights into the Superior DNA Delivery Efficiency of Multicomponent Lipid Nanoparticles: An In Vitro and In Vivo Study

Author

Quagliarini, Erica, primary, Wang, Junbiao, additional, Renzi, Serena, additional, Cui, Lishan, additional, Digiacomo, Luca, additional, Ferri, Gianmarco, additional, Pesce, Luca, additional, De Lorenzi, Valentina, additional, Matteoli, Giulia, additional, Amenitsch, Heinz, additional, Masuelli, Laura, additional, Bei, Roberto, additional, Pozzi, Daniela, additional, Amici, Augusto, additional, Cardarelli, Francesco, additional, Marchini, Cristina, additional, and Caracciolo, Giulio, additional

Published
2022
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13. Multiplexed Detection of Pancreatic Cancer by Combining a Nanoparticle-Enabled Blood Test and Plasma Levels of Acute-Phase Proteins

Author

Caputo, Damiano, primary, Coppola, Alessandro, additional, Quagliarini, Erica, additional, Di Santo, Riccardo, additional, Capriotti, Anna Laura, additional, Cammarata, Roberto, additional, Laganà, Aldo, additional, Papi, Massimiliano, additional, Digiacomo, Luca, additional, Coppola, Roberto, additional, Pozzi, Daniela, additional, and Caracciolo, Giulio, additional

Published
2022
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15. Efficient Delivery of DNA Using Lipid Nanoparticles

Author

Cui, Lishan, primary, Renzi, Serena, additional, Quagliarini, Erica, additional, Digiacomo, Luca, additional, Amenitsch, Heinz, additional, Masuelli, Laura, additional, Bei, Roberto, additional, Ferri, Gianmarco, additional, Cardarelli, Francesco, additional, Wang, Junbiao, additional, Amici, Augusto, additional, Pozzi, Daniela, additional, Marchini, Cristina, additional, and Caracciolo, Giulio, additional

Published
2022
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18. Reproducibility of Biomolecular Corona Experiments: A Primer for Reliable Results.

Author

Giulimondi, Francesca, Quagliarini, Erica, Digiacomo, Luca, Renzi, Serena, Palmieri, Valentina, Papi, Massimiliano, Pozzi, Daniela, and Caracciolo, Giulio

Subjects
*SCIENTIFIC community, *RESEARCH teams, *STANDARDIZATION, *EXPERIMENTAL design, *NANOSTRUCTURED materials
Abstract

The biomolecular corona is a key component controlling the identity of nanomaterials in physiological environments. Studies aimed at identifying factors shaping the biomolecular corona have proliferated in the last decade but have been performed by research groups with different backgrounds. Efforts made within the scientific community to guarantee the reproducibility of experimental data have identified protocol standardization as an indispensable step for advancing knowledge in this arena. To contribute to fulfill this gap, here the relevance of interoperator variability in biomolecular corona studies and the benefits arising from automated systems usage are explored. Moreover, the role of molecular crowding during nanoparticle‐biofluid incubation and the effect of washing the pellet during corona isolation are thoroughly investigated. It is believed that the findings will help researchers enhance the accuracy of experimental design and reporting. [ABSTRACT FROM AUTHOR]

Published
2023
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21. Microfluidic Formulation of DNA-Loaded Multicomponent Lipid Nanoparticles for Gene Delivery

Author

Quagliarini, Erica, primary, Renzi, Serena, additional, Digiacomo, Luca, additional, Giulimondi, Francesca, additional, Sartori, Barbara, additional, Amenitsch, Heinz, additional, Tassinari, Valentina, additional, Masuelli, Laura, additional, Bei, Roberto, additional, Cui, Lishan, additional, Wang, Junbiao, additional, Amici, Augusto, additional, Marchini, Cristina, additional, Pozzi, Daniela, additional, and Caracciolo, Giulio, additional

Published
2021
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24. Protein corona profile of graphene oxide allows detection of glioblastoma multiforme using a simple one-dimensional gel electrophoresis technique: a proof-of-concept study

Author

Di Santo, Riccardo, primary, Quagliarini, Erica, additional, Digiacomo, Luca, additional, Pozzi, Daniela, additional, Di Carlo, Angelina, additional, Caputo, Damiano, additional, Cerrato, Andrea, additional, Montone, Carmela Maria, additional, Mahmoudi, Morteza, additional, and Caracciolo, Giulio, additional

Published
2021
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31. Functionalization of gold nanoparticles with peptidomimetics as a targeted therapy for multiple sclerosis

Author

Ramírez-Jiménez, Rafael, Quagliarini, Erica, Bussolari, Francesca, Asín, Laura, Martín-Rapún, Rafael, Gentilucci, Luca, Fuente, Jesús M. de la, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), and Gobierno de Aragón

Abstract

Póster presentado a la 2nd Spanish Conference on Biomedical Applications of Nanomaterials (SBAN), celebrada en Madrid del 6 al 7 de junio de 2019., Multiple Sclerosis (MS) is an inflammatory autoimmune disease of the central nervous system that can lead to severe disability. The wide variety of symptoms characteristic for MS such as numbness, weakness, paresis, visual problems, severe fatigue and depression and also intellectual and cognitive impairments are caused by by brain and spinal cord's inflammation that damages the myelin sheath. During the last years, significant progress has been made in understanding how pathogenic leukocytes migrate from the periphery to the CNS, and the critical roles played by the α4ß1and α4ß7 integrins have been studied extensively in animal models, and in patients in clinical trials. Thus, the counter ligands of these integrins (VCAM1 and MAdCAM1) are a perfect target to prevent the inflammatory process. Gold nanoparticles (AuNPs) show low toxicity and excellent optical properties, they represent a very powerful tool in imaging and therapy applications. In this work, we propose the functionalization of AuNPs with five different integrins ligand-like peptidomimetics to target the pathogenic leukocytes and create a challenging device for imaging and therapy of MS. Preliminary outcomes in in vitro experiments will be presented in order to lay the foundations for future study., This project has received funding from MCINN (Spain) (project "SAF2014-54763-C2-1-R"), from Gobierno de Aragón (predoctoral contract of Rafael Ramírez Jiménez) and from Fondo Social of Gobierno de Aragón (Spain).

Published
2019

32. Funcionalización de nanopartículas de oro con peptidomiméticos como posible terapia para la esclerosis múltiple

Author

Ramírez-Jiménez, Rafael, Quagliarini, Erica, Bussolari, Francesca, Asín, Laura, Martín-Rapún, Rafael, Gentilucci, Luca, and Fuente, Jesús M. de la

Abstract

Resumen del póster presentado a la 8ª Jornada de Jóvenes Investigadores (Química y Física) de Aragón, celebrada en Zaragoza el dia 22 de noviembre de 2018., La esclerosis múltiple es una enfermedad crónica autoinmune que se caracteriza por la desmielinización del sistema nervioso central lo que daña severamente su funcionalidad. Se relaciona con procesos de inflamación causados por linfocitos T autorreactivos que sobreexpresan las integrinas α4β1 y α4β7, gracias a las cuales llegan al cerebro cruzando la barrera hematoencefálica. Así, los ligandos de estas integrinas (VCAM1 and MAdCAM1) pueden ser una diana perfecta para prevenir estos procesos de inflamación. Las nanopartículas de oro presentan baja toxicidad y propiedades que las hacen idóneas para aplicaciones en terapia y diagnóstico. En este trabajo proponemos la funcionalización con seis peptidomiméticos de los ligandos VCAM1 and MAdCAM1 para que interaccionen con las integrinas presentes en células T autorreactivas y crear un instrumento de imagen y terapia de la esclerosis múltiple. Los peptidomiméticos se acoplaron sobre nanoesferas de oro de 14 nm recubiertas con ácido ω-mercaptopolietilenglicol-α-carboxílico. La funcionalización se llevó a cabo a través de formación de un enlace amida para lo que se estudiaron diferentes condiciones de reacción. Se realizó la caracterización físico-química de las nanopartículas funcionalizadas y se estudió su internalización en células Jurkat (línea inmortalizada de linfocitos T humanos) encontrando una mayor internalización de las nanopartículas funcionalizadas.

Published
2018

34. Synergistic Analysis of Protein Corona and Haemoglobin Levels Detects Pancreatic Cancer.

Author

Caputo, Damiano, Digiacomo, Luca, Cascone, Chiara, Pozzi, Daniela, Palchetti, Sara, Di Santo, Riccardo, Quagliarini, Erica, Coppola, Roberto, Mahmoudi, Morteza, and Caracciolo, Giulio

Subjects
PROTEIN analysis, PANCREATIC tumors, HEMOGLOBINS, POINT-of-care testing, EARLY detection of cancer, DUCTAL carcinoma, TUMOR markers, NANOPARTICLES
Abstract

Simple Summary: Pancreatic ductal adenocarcinoma (PDAC) is often diagnosed at an advanced stage and is burdened by poor prognosis. Its early diagnosis is rare because the disease is frequently asymptomatic for a long time. Our research aims to identify a useful and simple diagnostic tool for early PDAC detection. The combination of user-friendly, nanotechnology-based tools and standard blood tests showed high accuracy in discriminating PDAC patients from healthy subjects. If confirmed in large-cohort studies, these findings could represent an innovative and non-invasive method with a potential impact in clinical practice in early detection of PDAC. Simultaneous detection of multiple analytes from a single biological sample is gaining more attention in the development of more reliable and point-of-care diagnostic devices. We developed a multiplexed strategy that combined outcomes of clinical biomarkers with analysis of the protein corona that forms around graphene oxide sheets upon exposure to patient's plasma. As a paradigmatic case study, we selected pancreatic ductal adenocarcinoma (PDAC), mainly because of the absence of effective detection strategies that resulted in an extremely low five-year survival rate after diagnosis (<10%). Association of protein corona analysis and haemoglobin levels discriminated PDAC patients from healthy volunteers in up to 90% of cases. If further confirmed in larger-cohort studies, this approach may be used in the detection of PDAC. [ABSTRACT FROM AUTHOR]

Published
2021
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35. Enhancing Immune Responses against SARS-CoV-2 Variants in Aged Mice with INDUK: A Chimeric DNA Vaccine Encoding the Spike S1-TM Subunits.

Author

Cui L, Wang J, Orlando F, Giacconi R, Malavolta M, Bartozzi B, Galeazzi R, Giorgini G, Pesce L, Cardarelli F, Quagliarini E, Renzi S, Xiao S, Pozzi D, Provinciali M, Caracciolo G, Marchini C, and Amici A

Abstract

Currently available vaccines against COVID-19 showed high efficacy against the original strain of SARS-CoV-2 but progressively lower efficacy against new variants. In response to emerging SARS-CoV-2 strains, we propose chimeric DNA vaccines encoding the spike antigen, including a combination of selected key mutations from different variants of concern. We developed two DNA vaccines, pVAX-S1-TM-D614G and pVAX-S1-TM-INDUK (INDUK), encoding the SARS-CoV-2 S1 spike subunit in fusion with the transmembrane region that allows protein trimerization as predicted by in silico analysis. pVAX-S1-TM-D614G included the dominant D614G substitution, while the chimeric vaccine INDUK contained additional selected mutations from the Delta (E484Q and L452R) and Alpha (N501Y and A570D) variants. Considering that aging is a risk factor for severe disease and that suboptimal vaccine responses were observed in older individuals, the immunogenicity of pVAX-S1-TM-D614G and INDUK was tested in both young and aged C57BL/6 mice. Two vaccine doses were able to trigger significant anti-SARS-CoV-2 antibody production, showing neutralizing activity. ELISA tests confirmed that antibodies induced by pVAX-S1-TM-D614G and INDUK were able to recognize both Wuhan Spike and Delta variant Spike as trimers, while neutralizing antibodies were detected by an ACE2:SARS-CoV-2 Spike S1 inhibitor screening assay, designed to assess the capacity of antibodies to block the interaction between the viral spike S1 protein and the ACE2 receptor. Although antibody titer declined within six months, a third booster dose significantly increased the magnitude of humoral response, even in aged individuals, suggesting that immune recall can improve antibody response durability. The analysis of cellular responses demonstrated that vaccination with INDUK elicited an increase in the percentage of SARS-CoV-2-specific IFN-γ producing T lymphocytes in immunized young mice and TNF-α-producing T lymphocytes in both young and aged mice. These findings not only hold immediate promise for addressing evolving challenges in SARS-CoV-2 vaccination but also open avenues to refine strategies and elevate the effectiveness of next-generation vaccines., Competing Interests: The authors declare no competing financial interest., (© 2024 The Authors. Published by American Chemical Society.)

Published
2024
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