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7 results on '"Qu Yan-gang"'

1. Quantum dots immunofluorescence histochemical detection of EGFR gene mutations in the non-small cell lung cancers using mutation-specific antibodies

Author

Qu,Yan-Gang, Zhang,Qian, Pan,Qi, Zhao,Xian-Da, Huang,Yan-Hua, Chen,Fu-Chun, Chen,Hong-Lei, Qu,Yan-Gang, Zhang,Qian, Pan,Qi, Zhao,Xian-Da, Huang,Yan-Hua, Chen,Fu-Chun, and Chen,Hong-Lei

Abstract

Yan-Gang Qu,1 Qian Zhang,2 Qi Pan,3 Xian-Da Zhao,4 Yan-Hua Huang,2 Fu-Chun Chen,3 Hong-Lei Chen41Department of Pathology, The Central Hospital of Enshi Autonomous Prefecture, Enshi, 2Department of Molecular Pathology, Wuhan Nano Tumor Diagnosis Engineering Research Center, Wuhan, Hubei, People’s Republic of China; 3Department of Thoracosurgery, Traditional Chinese Medical Hospital of Wenling, Wenling, Zhejiang, People’s Republic of China; 4Department of Pathology, School of Basic Medical Science, Wuhan University, Wuhan, Hubei, People’s Republic of ChinaBackground: Epidermal growth factor receptor (EGFR) mutation status plays an important role in therapeutic decision making for non-small cell lung cancer (NSCLC) patients. Since EGFR mutation-specific antibodies (E746-A750del and L858R) have been developed, EGFR mutation detection by immunohistochemistry (IHC) is a suitable screening test. On this basis, we want to establish a new screening test, quantum dots immunofluorescence histochemistry (QDs-IHC), to assess EGFR gene mutation in NSCLC tissues, and we compared it to traditional IHC and amplification refractory mutation system (ARMS).Materials and methods: EGFR gene mutations were detected by QDs-IHC, IHC, and ADx-ARMS in 65 cases of NSCLC composed of 55 formalin-fixed, paraffin-embedded specimens and ten pleural effusion cell blocks, including 13 squamous cell carcinomas, two adenosquamous carcinomas, and 50 adenocarcinomas.Results: Positive rates of EGFR gene mutations detected by QDs-IHC, IHC, and ADx-ARMS were 40.0%, 36.9%, and 46.2%, respectively, in 65 cases of NSCLC patients. The sensitivity of QDs-IHC when detecting EGFR mutations, as compared to ADx-ARMS, was 86.7% (26/30); the specificity for both antibodies was 100.0% (26/26). IHC sensitivity was 80.0% (24/30) and the specificity was 92.31% (24/26). When detecting EGFR mutations, QDs-IHC and ADx-ARMS had perfect consistency (κ=

Published
2014

3. Research of symmetrical voltages and phase of three-phase inverter with dual-loop control

Author

Zou Yunping, Qu Yan-gang, Chen Yao-jun, Zhong Yan-ping, and Lai Xiang-dong

Subjects
Engineering, business.industry, Circuit design, Topology (electrical circuits), Hardware_PERFORMANCEANDRELIABILITY, Magnetic circuit, Phase-locked loop, Sine wave, Control theory, Hardware_INTEGRATEDCIRCUITS, Inverter, business, Pulse-width modulation, Voltage
Abstract

In terms of the conventional inverter doesn't match all unbalanced loads, a topology and strategy of nature- decoupling in circuit and magnetic circuit is proposed for aeronautic and military equipment field. Each phase voltage is controlled independently by PI controller and double-loop controller with voltage and current instantaneous feedback, and phase angle by closed-loop control with PLL , which make the output voltage and phase angle track the references sine wave. Pulse width and phase angle of reference sine wave are modulated by voltage-loop and phase-loop, which ensures phase and voltage of the inverter to symmetry. At first, output unsymmetrical mechanism of a traditional inverter is analyzed in this paper; Second, its main circuit topology, construction of voltage current feedback circuits, its control principle and implementation method are discussed; then, its mathematic models are set up and analyzed; At last, simulation and experiment results of 30 kW three-phase inverter is obtained that proves this circuit design to be feasible.

Published
2008

4. A feed-forward compensation strategy of SPWM Matrix Converter under abnormal input voltage conditions

Author

Zou Yunping, Chen Yao-jun, Zhong Yan-ping, Lai Xiang-dong, and Qu Yan-gang

Subjects
Engineering, business.industry, Feed forward, Compensation (engineering), Control theory, Compensation principle, Line (geometry), Electronic engineering, business, MATLAB, computer, Realization (systems), Pulse-width modulation, computer.programming_language, Voltage
Abstract

The feed-forward control scheme based on SPWM matrix converter is proposed. As a device without DC line, the AC line side abnormal inputs can deteriorate its output characteristics. Tri-carrier is prior modulated by measured envelop of line voltages, to let the pulse width of SPWM get different compensation in each pulse. This paper analyzes compensation principle, introduces realization method, and compares output behaviors of without compensation with compensation by Matlab/Simulink. Analyses and simulations show that the scheme modifies effectively output characteristics of MC (Matrix Converter) under abnormal input conditions. It is simple not only easy to realize through hardware but also to digital, and simple algorithm.

Published
2008

7. Quantum dots immunofluorescence histochemical detection of EGFR gene mutations in the non-small cell lung cancers using mutation-specific antibodies.

Author

Qu YG, Zhang Q, Pan Q, Zhao XD, Huang YH, Chen FC, and Chen HL

Subjects
Antibodies, Monoclonal, ErbB Receptors chemistry, Humans, Mutation genetics, Real-Time Polymerase Chain Reaction, Carcinoma, Non-Small-Cell Lung chemistry, ErbB Receptors genetics, Fluorescent Antibody Technique methods, Lung Neoplasms chemistry, Quantum Dots chemistry
Abstract

Background: Epidermal growth factor receptor (EGFR) mutation status plays an important role in therapeutic decision making for non-small cell lung cancer (NSCLC) patients. Since EGFR mutation-specific antibodies (E746-A750del and L858R) have been developed, EGFR mutation detection by immunohistochemistry (IHC) is a suitable screening test. On this basis, we want to establish a new screening test, quantum dots immunofluorescence histochemistry (QDs-IHC), to assess EGFR gene mutation in NSCLC tissues, and we compared it to traditional IHC and amplification refractory mutation system (ARMS)., Materials and Methods: EGFR gene mutations were detected by QDs-IHC, IHC, and ADx-ARMS in 65 cases of NSCLC composed of 55 formalin-fixed, paraffin-embedded specimens and ten pleural effusion cell blocks, including 13 squamous cell carcinomas, two adenosquamous carcinomas, and 50 adenocarcinomas., Results: Positive rates of EGFR gene mutations detected by QDs-IHC, IHC, and ADx-ARMS were 40.0%, 36.9%, and 46.2%, respectively, in 65 cases of NSCLC patients. The sensitivity of QDs-IHC when detecting EGFR mutations, as compared to ADx-ARMS, was 86.7% (26/30); the specificity for both antibodies was 100.0% (26/26). IHC sensitivity was 80.0% (24/30) and the specificity was 92.31% (24/26). When detecting EGFR mutations, QDs-IHC and ADx-ARMS had perfect consistency (κ  =0.882; P<0.01). Excellent agreement was observed between IHC and ADx-ARMS when detecting EGFR mutations (κ  =0.826; P<0.01)., Conclusion: QDs-IHC is a simple and standardized method to detect EGFR mutations with its high sensitivity and specificity, as compared with real-time polymerase chain reaction. In addition, the development of specific antibodies against EGFR mutation proteins might be useful for the diagnosis and treatment of lung cancer.

Published
2014
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