1. Preparation of ceftiofur-encapsulated hen-egg low-density lipoproteins and their antibacterial effects on intracellular Staphylococcus aureus.
- Author
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Zhao Y, Mao W, Liu B, Wang YF, Zhang SY, Guo LL, Qian YH, Gong ZG, Zhao JM, Yang XL, Qu GG, Hasi SR, Bai YT, and Cao JS
- Subjects
- Animals, Mice, Cattle, Female, Drug Carriers chemistry, Staphylococcal Infections drug therapy, Eggs, Staphylococcus aureus drug effects, Lipoproteins, LDL metabolism, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Cephalosporins pharmacology, Cephalosporins pharmacokinetics, Cephalosporins chemistry, Chickens
- Abstract
Hen egg low-density lipoprotein (heLDL), as alternative of serum-derived LDL, was used as drug delivery system of ceftiofur (CEF). The CEF-loaded hen egg low-density lipoprotein (CEF-heLDL) with complete apolipoprotein structure and high drug loading rate was synthesized, possesses suitable particle size. CEF-heLDL undergoes cellular uptake and colocalizes with lysosomes in vitro. An intracellular infection model of the bovine endometrial epithelial cells and a coeliac-induced inflammation model of mice by Staphylococcus aureus (S. aureus) were established, and significantly lower intracellular S. aureus levels of CEF-heLDL group than CEF-free group (P < 0.001) was observed. The antibacterial efficacy was sustained for 24 h. Up to 400 mg/kg of CEF-heLDL, 20 times the clinical practice, were intraperitoneally administrated, and no significant toxicity signs on mice were observed. HeLDLs is an effective, safe, and cheap drug carrier, and could also be used for transmembrane delivering other antibiotics., Competing Interests: Declaration of competing interest CJS, MW, BYT, ZSY, LB, YJL, HYL, HSSR, LPF, WYF, and Gll are co-inventors on a patent application for a fusion method for yolk low-density lipoprotein and ceftiofur, which is available for licensing. The other authors declare no competing interests., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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