1. Three-dimensional architecture of granulosa cell derived from oocyte cumulus complex, revealed by FIB-SEM
- Author
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Chongyi Shu, Yiqi Yu, Xiaopan Chen, Jiansheng Guo, Yier Zhou, Dandan Wu, Tianyun Yang, Yuhang Fan, Qiongxiao Huang, and Jing Shu
- Subjects
Oocyte cumulus complex ,Granulosa cells ,Focused ion beam scanning electronmicroscopy ,3D reconstruction ,Organelles ,Intercellular connections ,Gynecology and obstetrics ,RG1-991 - Abstract
Abstract The oocyte cumulus complex is mainly composed of an oocyte, the perivitelline space, zona pellucida and numerous granulosa cells. The cumulus granulosa cells (cGCs) provide a particularly important microenvironment for oocyte development, regulating its growth, maturation and meiosis. In this study, we studied the internal structures and cell-to-cell connections of mouse cGCs using focused ion beam scanning electron microscopy (FIB-SEM). We reconstructed three-dimensional models to display characteristic connections between the oocyte and cGCs, and to illustrate various main organelles in cGCs together with their interaction relationship. A special form of cilium identified in granulosa cell was never reported in previous literature.
- Published
- 2023
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