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Teicoplanin, a glycopeptide antibiotic, is used for the treatment of severe staphylococcal infections. Teicoplanin is reported to have an ototoxic potential but its toxic effects on cochlea hair cells (HCs) remains unclear. TP53-induced glycolysis and apoptosis regulator (TIGAR) plays a key role to promote cell survival, our previous study shown that TIGAR protected inner ear spiral ganglion neuron against cisplatin injury. However, the role of TIGAR in mammalian HCs damage has not been explored yet. In this study, firstly, we found that teicoplanin induced significant cell loss of both HEI-OC1 cells and cochlea HCs in a dose-dependent manner in vitro. Next, we discovered that the expression of TIGAR was significantly decreased after teicoplanin treatment in HCs and HEI-OC1 cells. To explore the role of TIGAR in inner ear after teicoplanin damage, the expression of TIGAR was upregulated via recombinant adenovirus or knocked down by shRNA in HEI-OC1 cells, respectively. We found that the overexpression of TIGAR increased cell viability, decreased apoptosis and reduced intracellular reactive oxygen species (ROS) level after teicoplanin injury, whereas downregulation of TIGAR by shRNA decreased cell viability, exacerbated apoptosis and elevated ROS level. Finally, antioxidant treatment with N-acetyl-L-cysteine lowered ROS level, rescued cell loss as well as restored p38/phosphorylation-p38 expression levels induced by TIGAR deficiency in HEI-OC1 cells after teicoplanin injury. This study provides evidences that TIGAR might be a new potential target for prevention from the teicoplanin-induced ototoxicity.

Post-traumatic stress disorder (PTSD) is a debilitating psychiatric disorder. It can be difficult to discern the symptoms of PTSD and obtain an accurate diagnosis. Different magnetic resonance imaging (MRI) modalities focus on different aspects, which may provide complementary information for PTSD discrimination. However, none of the published studies assessed the diagnostic potential of multimodal MRI in identifying individuals with and without PTSD. In the current study, we investigated whether the complementary information conveyed by multimodal MRI scans could be combined to improve PTSD classification performance. Structural and resting-state functional MRI (rs-fMRI) scans were conducted on 17 PTSD patients, 20 trauma-exposed controls without PTSD (TEC) and 20 non-traumatized healthy controls (HC). Gray matter volume (GMV), amplitude of low-frequency fluctuations (ALFF), and regional homogeneity were extracted as classification features, and in order to integrate the information of structural and functional MRI data, the extracted features were combined by a multi-kernel combination strategy. Then a support vector machine (SVM) classifier was trained to distinguish the subjects at individual level. The performance of the classifier was evaluated using the leave-one-out cross-validation (LOOCV) method. In the pairwise comparison of PTSD, TEC, and HC groups, classification accuracies obtained by the proposed approach were 2.70, 2.50, and 2.71% higher than the best single feature way, with the accuracies of 89.19, 90.00, and 67.57% for PTSD vs. HC, TEC vs. HC, and PTSD vs. TEC respectively. The proposed approach could improve PTSD identification at individual level. Additionally, it provides preliminary support to develop the multimodal MRI method as a clinical diagnostic aid.

To realize the accurate positioning and quantitative volume measurement of tumor in head and neck tumor CT images, we proposed a level set method based on augmented gradient. With the introduction of gradient information in the edge indicator function, our proposed level set model is adaptive to different intensity variation, and achieves accurate tumor segmentation. The segmentation result has been used to calculate tumor volume. In large volume tumor segmentation, the proposed level set method can reduce manual intervention and enhance the segmentation accuracy. Tumor volume calculation results are close to the gold standard. From the experiment results, the augmented gradient based level set method has achieved accurate head and neck tumor segmentation. It can provide useful information to computer aided diagnosis.

Magnetic resonance imaging (MRI) is by nature a multi-modality technique that provides complementary information about different aspects of diseases. So far no attempts have been reported to assess the potential of multi-modal MRI in discriminating individuals with and without migraine, so in this study, we proposed a classification approach to examine whether or not the integration of multiple MRI features could improve the classification performance between migraine patients without aura (MWoA) and healthy controls. Twenty-one MWoA patients and 28 healthy controls participated in this study. Resting-state functional MRI data was acquired to derive three functional measures: the amplitude of low-frequency fluctuations, regional homogeneity and regional functional correlation strength; and structural MRI data was obtained to measure the regional gray matter volume. For each measure, the values of 116 pre-defined regions of interest were extracted as classification features. Features were first selected and combined by a multi-kernel strategy; then a support vector machine classifier was trained to distinguish the subjects at individual level. The performance of the classifier was evaluated using a leave-one-out cross-validation method, and the final classification accuracy obtained was 83.67% (with a sensitivity of 92.86% and a specificity of 71.43%). The anterior cingulate cortex, prefrontal cortex, orbitofrontal cortex and the insula contributed the most discriminative features. In general, our proposed framework shows a promising classification capability for MWoA by integrating information from multiple MRI features.