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Chitin deacetylase (CDA) inhibitors were developed as novel antifungal agents because CDA participates in critical fungal physiological and metabolic processes and increases virulence in soilborne fungal pathogens. However, few CDA inhibitors have been reported. In this study, 150 candidate CDA inhibitors were selected from the commercial Chemdiv compound library through structure-based virtual screening. The top-ranked 25 compounds were further evaluated for biological activity. The compound J075-4187 had an IC50 of 4.24 ± 0.16 μM for

Myrosinase can hydrolyze glucosinolates to generate isothiocyanates, which have cancer prevention and anti-cancer properties. The main sources of myrosinase are cruciferous plants. To further improve the efficiency of isothiocyanates preparation, it is necessary to explore novel sources of myrosinases. In this study, we described a bacterium, Shewanella baltica Myr-37, isolated from marine mud, capable of producing a novel myrosinase (Smyr37) with a molecular weight of 100 kDa. The crude enzyme of Smyr37 showed the highest activity at 50 °C and pH 8.0. The sinigrin- and glucoraphanin-hydrolyzing activities of Smyr37 were 6.95 and 5.87 U/mg, respectively. Moreover, when the reaction temperature was 40 °C and pH was 7.0, the crude enzyme of Smyr37 could efficiently degrade glucoraphanin into sulforaphane within 25 min with a yield of 0.57 mg/mL. The corresponding conversion efficiency of sulforaphane from glucoraphanin was 89%. In summary, S. baltica Myr-37 myrosinase Smyr37, a novel myrosinase, can be used in the preparation of isothiocyanates.

With the stimulating demand of consumers for personalized garments, mass customization is gradually becoming prevalent. However, the automatic generation of personalized garment patterns remains core to mass customization. In this article, we propose a novel parametric apparel pattern-making method and achieve the automatic generation of personalized garment patterns in batches. First, we review the basic principle of biarc and present a new parametric pattern-making method based on biarc and ezdxf. Second, 1000 3D human models randomly generated by the SMPL-X parametric human model are clustered into 10 classes with the k-means clustering method, and 10 representative human models are selected from each cluster to generate their personalized garment pattern. Finally, the rationality of the personalized garment pattern is verified by virtual fitting. Our method has several advantages: (1) the proposed parametric biarc can be easily used to build parametric garment patterns without being limited by the style of the garment, (2) the proposed method is capable of generating personalized garment patterns in batches by imputing measurements from large numbers of individuals, and it only takes very little time, and (3) the personalized garment patterns can fit human bodies very well. The proposed method can be used to build parametric garment patterns and to achieve the batch generation of personalized patterns, improving the efficiency of garment customization and the quality of final products.

Developing chitinase suitable for the bioconversion of chitin to chitin oligosaccharides has attracted significant attention due to its benefits in environmental protection. In this study, chitinase from Aeromonas media CZW001 (AmChi) was purified and characterized. The molecular weight of AmChi was approximately 40 kDa. AmChi exhibited maximum catalytic activity at pH 8.0 with an optimum temperature of 55°C and showed broad stability between 15 and 65°C and between pH 5.0 and 9.0. AmChi was activated by Mg