1. Mechanism of Inhibitory Effect of Essential Oil from Valerianae Jatamansi Rhizoma et Radix on Activation of Microglia.
- Author
-
Qingde LONG, Nanyun YANG, Fanli ZENG, Xu ZHANG, Xiaoying WU1, and Xiangchun SHEN
- Subjects
- *
ESSENTIAL oils , *MICROGLIA , *GENE expression , *INTERLEUKIN-6 , *WESTERN immunoblotting , *PROTEIN expression , *INTERLEUKIN-1 - Abstract
[ Objectives ] To investigate the inhibitory effect and possible mechanism of essential oil from Valerianae Jatamansi Rhizoma et Radix on microglia activation induced by lipopolysaccharide $ LPS ). [ Methods ] The LPS-induced microglia activation model was established and treated with different doses of essential oil from Valerianae Jatamansi Rhizoma et Radix. MTT assay was used to detect cell viability, ELISA to detect IL-6 secretion, RT-PCR to detect mRNA expression levels of IL-6, IL-1, NF-kB, and IkBcc, Western blotting to detect the protein expression of IL-6, IL-1,NF-kB, IkBcc, and their phosphorylated products. [ Results] Compared with the norma control group, the model group showed increased IL-6 content $ A<0.01), upregulated mRNA and protein levels of IL-6, IL-1", and NF-kB $ A<0.01), and elevated ratio of P-IkBcc/IkBcc $ A < 0. 05). Compared with the model group, 4 and 2 xg /L essential oil from Valerianae Jatamansi Rhizoma et Radix reduced the content of IL-6 $ A < 0. 05), while 4, 2, and 1 xg /L essential oil from Valerianae Jatamansi Rhizoma et Radix down-regulated the mRNA and protein levels of IL-6, IL-1 and NF-kB to varying degrees $ A < 0. 05 or A < 0. 01), up-regulate the mRNA expression of IkBcc $ A <0. 05 or A <0.01), and decreased the ratio of P-IkBcc/IkBcc $ A <0.05 or A <0.01). [ Conclusions] Essential oil from Valerianae Jatamansi Rhizoma et Radix can inhibit LPS-induced microglia activation, and its mechanism may be related to the inhibition of the NF-kB/IkBcc signaling pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF