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Oncogene HER2 is amplified in 20%-25% of human breast cancers and 6.1%-23.0% of gastric cancers, and HER2-directed therapy significantly improves the outcome for patients with HER2-positive cancers. However, drug resistance is still a clinical challenge due to primary or acquired mutations and drug-induced negative regulatory feedback. In this study, we discovered a potent irreversible HER2 kinase inhibitor, CHMFL-26, which covalently targeted cysteine 805 of HER2 and effectively overcame the drug resistance caused by HER2 V777L, HER2 L755S, HER2 exon 20 insertions, and p95-HER2 truncation mutations. CHMFL-26 displayed potent antiproliferation efficacy against HER2-amplified and mutant cells through constant HER2-mediated signaling pathway inhibition and apoptosis induction. In addition, CHMFL-26 suppressed tumor growth in a dose-dependent manner in xenograft mouse models. Together, these results suggest that CHMFL-26 may be a potential novel anti-HER2 agent for overcoming drug resistance in HER2-positive cancer therapy.

Through the Suizhong 36-1 oilfield core acidification experiments, we studied the different acid systems on the dissolution rate of rock samples and determine the preferred suitable for different circumstances Suizhong 36-1 oilfield cuttings acid system[1].

For the unique geological features in Haita area, we optimized the synthesis conditions of slurry fluid loss additive ZJK103 and evaluated its performance. The experimental results showed that under different water conditions, water quality had no effect on fluid loss of ZJK103; The fluid loss additive had no adverse impact on compressive strength of cement stone; With same mixing water and different conserving water, the compressive strength did not change much. ZJK103 had better thickening effect than other average fluid loss additives. According to the performance of slurry system, the indoor synthetic slurry filtrate reducer could meet the quality requirements of Haita region cementing.

Low temperature early strength cement slurry is a slurry in order to meet the well cementation in low-temperature conditions. Daqing weather is characterized by the silica fume, solid alcohols, amines and anionic polymers prepared a certain proportion, got a new low temperature early strength agent, It can greatly accelerate the hydration rate of cement and promote the formation of the cement paste early strength; and no chlorine ions, able to overcome the problem caused by traditional chlorine early strength agent casing corrosion.

The solid free completion fluid is the KCl-polymer system, it has the good inhibitory and stabilize the clay. The corrosion rate is 0.0775 g/m2·h, for different permeability core, the degree of injury is less than 20%.Use completion fluid to reduce oil layer damage of main mechanism for the effect of k+, inhibition of polymer and the synergy of K + and polymer.

Gemini surfactants are chemical compounds known as new generation of surfactants that their molecules has two hydrophilic groups ,two hydrophobic groups and a spacer.They have low CMC value and high surface activity.Gemini surfactants are inapproachable by any surfactants that are used for TOR nowadays.This essay is meant to solve the problems faced in the territory by using the remarkably high surface activity,introducing Gemini surfactants to TOR and launch series of experiments.Meanwhile,it provides technical and theoretical support for applying Gemini surfactants into the field.

Aimed at the cement slurry leak problem in the leakiness area cementing of Jilin oilfield, the fiber cement slurry system was developed. The general performance of fiber cement slurry, such as the pressure strength, thickness time, filter loss, flowing ability and water separate rate, etc was analysis in the room condition. The preventing and block leak ability was evaluated. The room and field tests prove that the ability of the fiber cement slurry is best. It can make the field cementing requirement in the leakiness formation and has the best effect of preventing and block leak ability.

In order to better protect the Daqing Oilfield Sanan oil field development, make experimental analysis and field applications of Sanan's core.Through the relationship between rheology and fluid loss ,achieve JGN best dosage of the entire completion fluid system, completion rheological law and evaluated regular patten.The effect of field application is obvious, improve strength of the oil production , the skin factor and injection pressure is reduced, and made better protect the reservoir effect.

The basic prescription of the potassium sulfate drilling fluid is: 5% bentonite+0.4%Na2CO3+5.0%K2SO4+1000ppmDCL+200ppmDesco+100ppmAlcome+500ppmSAP+1000ppmLT+1000ppmKEL. The content of K+ is 50000mg/L. The drilling fluid has appropriate viscosity, gel and filter loss. The shale rolling recovery rate is bigger than 85%. The static swelling rate is smaller than 2%. The corn damage is less than 20%. The mechanism of protect formation is the K+ ability of preventing the clay from hydrating and swelling, the adsorption ability of formation protecting agent and the cooperating role of K+ and formation protecting agent. The potassium sulfate drilling fluid was used in fang-3 outer section of the Song-fang-tun oilfield. It was shown that the drilling fluid could meet the drilling demand in this oilfield.

An acid self-diverting agent compound by betaine type surfactant and quaternary ammonium salt type surfactant was synthesized and evaluated. Found that when the pH value was higher than 1, acid viscosity rose rapidly, and the pH value up to 3, acid viscosity reached its maximum value (300mPa s), and can effectively made the acid diverse.When the pH value was 6, the system completely gelouted, easy to flowback; When added the self-diverting additives, acid system had better temperature resistance; With the elevated of concentration of the self-diverting agent, the apparent viscosity of acid increased, when the concentration of the self-diverting agent was more than 5%, the acid apparent viscosity increased little, gradually stabilized, but all above 300mPas, meet the industry standard requirements.

Determine the best formula of the double excitation inorganic adjustable plugging agent: 15% slag + 0.8% activator + 4% dispersant + 2.5% retarder, with a curing time of 32h, and a curing strength over 1.4Mpa. The initial viscosity of adjustable plugging agent is 36mPa.s, and after curing for eighteen hours in 85 degrees Celsius , viscosity is less than 320mPa.s. For cores with different permeability, the water plugging rate of adjustable plugging agent is more than 90%.

In this paper,we used the heavy oil of Du 84,53-55 well in Liaohe oil field,analyzed of the impact factors of the heavy oil emulsion viscosity.When the droplet size of the emulsion is reduced,the viscosity of the heavy oil emulsion increases,and the non-Newtonian obvious.Heavy oil emulsions exhibit Newtonian fluid properties at low shear rate,exhibits shear-thinning properties in the heavy oil emulsion under high shear rate.Heavy oil emulsion viscosity decreases with the increasing concentration of viscosity reducer,when the viscosity reducer concentration of more than 0.25%,heavy oil emulsion viscosity declines slowed.

A long period of development in Haituozi exploration area, downhole conditions are constantly changing, increasingly complex, and the original cementing technology is difficult to solve the region's low pressure leakage problems. For the special circumstances of the region, we set up a special research team, focus on starting from the the preferred cement slurry system, and improve stability, toughness, plugging, leak-proof ability of the slurry system performance; Another optimization cementing design and perfect cementing supporting measures effectively solve the region's dropout problem.

In-depth development of a block of Sudan, effectively develop the rich reserves of the major reservoirs, increase crude oil production, and reduce the cost of drilling.This horizon has a high penetration, high formation temperature, easy to collapse, gas channeling and complex problem, there is a great test of this case to horizontal wells cementing.Therefore, develop a new expansion slurry system,which has the feature of high-strength, low water loss, gas channeling effect, solves the region problems of cementing technical. A block of Sudan formation pressure coefficient is low, fracture pressure is small, strata is sulfur loose sandstone, and stratigraphic diagenetic is poor, easy to collapse and necking.With the deepening of the development ,these issues are more and more obvious.In order to achieve the best results in the horizontal well cementing operations, we research from geological data, well structure, cementing formulations and process in-depth and meticulous. Solve the high permeability formation temperature, easy to collapse, gas channeling technical problems, develope a new type of high-strength, low water loss, micro-expansion slurry system, and effectively solve the block problem of horizontal wells cementing .

Adsorption isotherms of new type cationic Gemini surfactant named CGS on quartz sand has been got by using solution concentration difference method. The effect of different factors on adsorption, such as temperature, pH value and salinity, are studied too. The adsorption isotherm of CGS on quartz sand accorded with Langmuir shape. The adsorption quantity increased with the increasing of surfactant concentration at an adagio rate, then the adsorption quantity increased fast, and it reached adsorption saturation when mass concentration was 150 mg/L, and saturated absorption was 6.3 mg/g. The adsorption quantity of CGS increased with the increasing of salt concentration. When heighten temperature and pH value, the adsorption quantity of CGS decreased.

In this paper, aiming at the Fuyu high angle holes cementing technical issues, in-depth study the quick-setting, high early strength and micro-expansion anti-channeling slurry system. Use this system in Fuyu region high angle holes cementing engineering, the completion of 10 high angle holes were all qualified, 8 of which were quality well, the pass rate of 100% and quality rate of 80%.Solve the difficult problem of the cementation quality and packer formation of the cement sheath, ensure the cementing Quality of Fuyu city high angle wells, which has an important guide and reference on Jilin Oilfield in the future development of the deep horizontal and cementing technology.

Cationic Gemini surfactant concentration, the inorganic salts added and the pH value of surface tension obtained cationic gemini surfactant critical micelle concentration is 0.4mmol / L;by adding three kinds of inorganic salts NaCl, MgCl2, and Na2SO4 ,which Na2SO4 has the greatest impact on surface tension, followed by MgCl2.The surface minimum tension of the pH ranged from 9 to 11 , indicating that the surface activity of cationic gemini surfactants achieved the highest.

A cationic Gemini surfactant ethane-1, 2-bis (Cetyl dimethyl brominated ammonium), denoted 16-2-16 was synthesized from ethane and Hexadecyl dimethyl amine. The properties of the product were characterized, and the physical and chemical properties were determined. The results showed that the synthetic product was the target product, and its surface tension was 3.4×10-4N/m, and the CMC was 2.2×10-4mol/L at 25°C. At the same time, its synergistic effect with common surfactants was studied; the results showed that the synthetic product had good synergic effect.

Liaohe oil field block 58 for Huancai, the efficiency of production of thickened oil is low, and the efficiency of displacement is worse, likely to cause other issues. Researching and developing an type of Heavy Oil Viscosity Reducer for exploiting. The high viscosity of W/O emulsion changed into low viscosity O/W emulsion to facilitate recovery, enhanced oil recovery. Through the experiment determine the viscosity properties of Heavy Oil Viscosity Reducer. The oil/water interfacial tension is lower than 0.0031mN•m-1, salt-resisting is good. The efficiency of viscosity reduction is higher than 90%, and also good at 180°C.

MFR high temperature profile control agent consisted of 6.5% wood Fortunately sulfonic acid calcium (Ca-LS) and 6.5% crosslinker modified furfural resin (MFR),and the gel solution formed a stable performance gel in the 150~300°C.The higher the temperature, the shorter the gelation time; and the longer the heating time, the higher the gel strength. The gel is very stable at 300°C, and is well in acid and salt resistance. The core experiments showed that the blocking agent's blockage ratio is more than 99% ; the units of the breakthrough pressure was greater than 100 kPa / cm,which the gel solution is a good performance of high-temperature profile control agent.

The main problem dehydration system of XinMu Oil Field at present is high dehydration temperatures,high the amount of demulsifier, increase output moisture content year by year and more and more frequent fluctuations.Through the reform to establish a dehydration subsystems,and composite demulsifier alone to deal with special oil to eliminate the influence of special crude oil to dehydration system.Filter out Daqing produced 169＃ demulsifier 150mg·L-1, when add oxalic acid 200mg·L-1 and fungicide 100mg·L-1 the demulsifier of good dehydration effect.After processing crude oil water content of less than 5%,sewage oil content of less than 20mg·L-1, which has solved the dehydration problems of troubled XinMu for many years.

The dehydrationrate of Daqing crude oil was not stable and the amount of chemical demulsifier used too much, which caused serious environmental pollution and enlarged the difficulty of dealing with crude oil. Therefore, PRJ-1 bio-demulsifier was selected to combine with chemical demulsifiers SP-169 and GT-D01, respectively, to resolve the crude oil emulsion of Daqing. The results showed that PRJ-1 can combine with SP-169 efficiently. The demulsification efficiency was high and dehydrated water was clear. However, the demulsification efficiency increased a little with GD-D01. The study shows that efficient synergetic effect between PRJ-1 and SP-169 was confirmed. The suitable proportionality of combinational demulsifier is 1:1 and the demulsifying effect can meet requirement of oilfield demulsifiers currently.

Bio-demulsifier has not been applied in industrial scale due to its production cost. In order to reduce the production cost and improve the efficiency of demulsification, cheap materials such as oilfield sludge, workshop waste oil and edible waste oil were used in experiments as carbon sources to culture PRJ-1 demulsifying bacteria. As a result, cell concentrations of 5.4g/L, 3.7g/L and 4.2g/L were gotten, which were higher than the concentration of 2.6g/L cultivated by using paraffin. PRJ-1 demulsifying bacteria were used to prepare the demulsifying solution with a concentration of 10g/L, which was used in the demulsification experiment on Liaohe crude oil with a water cut of 80%. With 100 ml/L being added into the crude oil, the demulsification efficiency at 12h reached 91%, which was higher than that of 100mg/L chemical demulsifier. Demulsification efficiency of 96% can be achieved by using the combination of 60ml/L demulsifying bacteria and 40mg/L chemical demulsifier, furthermore, the demulsifying speed was higher and dehydrated water was clearer.

Persicae Semen (PS), a traditional Chinese medicine, has been widely used for the syndrome of blood stasis in China since the Eastern Han Dynasty. In the present study, we developed an HPLC-UV fingerprint analysis method for the quality control of PS. The HPLC fingerprint was performed on Shimadzu Inertsil C18 column (4.6 mm x 250 mm, 5 microm) at 35 degrees C. The mobile phases were composed of acetonitrile and water using a gradient elution. The flow rate was 1.0 mL x min(-1), and the detection wavelength was set at 210 nm. The fingerprint method was validated according to the Guidelines for Traditional Chinese Medicine Injection Fingerprint, and applied to determine 41 batches representative herbs collected from Xinjiang of China. The chromatographic peaks were characterized by UPLC-Q-TOF-MS, and nine of them were identified by comparison with the literature and/or reference standards. In order to classify and assess the samples, hierarchical clustering analysis and partial least square discriminant analysis were performed based on the common chromatographic peaks, and the samples were geographically classified into two classes, with six chemical compounds as classification markers which were significantly different between the two classes (P0.05).

The sample digested by microwave are of some merits: simple, rapid, saving agents, nonpollution and being easily digested. Microwave-digested sample with HNO3-H2O2 and AAS method were established for the determination of Fe, Ca, Mg, Zn, Mn, Cu, Na, K, Ni, Cd, Pb and Cr in ephedrine extraction. In this paper, the selection of digestion conditions, including pressure, power, acids and time of digestion was evaluated. According to standard sample GBW08501 Tea-leaves, we checked the method accuracy and precision. The accuracy values are in the range of 91.1%-105%. The RSDs of flame atomic spectrometry and graphite furnace atomic spectrometry are in the range of 0.2%-2.3% and less than 7%, respectively. The results are very consistent with the the reference values. It proved that the method has satisfactory precision and accuracy. The proposed method is simple, rapid, of nonpollution and safe. It was has been applied to the determination of trace elements in ephedrine extraction and provided useful data for production in factory.