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OBJECTIVES Ascending to altitudes >2500 m may lead to acute mountain sickness (AMS). METHODS The demographics, height, weight, body mass index (BMI), smoking, and alcohol consumption of 104 healthy controls were collected in Chengdu (500 m). Heart rate (HR), saturation of pulse oxygen (SpO2), and AMS-related symptoms were collected in Hoh Xil (4200 m). A headache with Lake Louise score ≥3 was defined as AMS. RESULTS The incidence of AMS was 60.58%. AMS group had a lower SpO2 and higher HR than non-AMS group. Alcohol consumption seemed a risk factor for AMS. There was no difference in the BMI, age, height, weight, and smoking between AMS and non-AMS groups. The most common AMS symptom was headache, followed by dyspnea, insomnia, dizziness, lassitude, and anorexia. Women were prone to suffer from dizziness. The value of SpO2 and HR was improved both in AMS and non-AMS groups after hypoxia acclimatization, and the value showed greater improvement in AMS group. Oxygen therapy decreased the AMS-induced tachycardia, which had no any effect on SpO2 and symptom alleviating time. CONCLUSION Lower SpO2 and higher HR following exposure to high altitude were associated with AMS susceptibility. The anthropometric data changes were larger in AMS group than non-AMS group before and after hypoxia acclimatization.

Objective The experiment aimed to determine the standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in 10 brown rice samples fed to pigs, and to construct predictive models for SID of CP and AA based on the physical characteristics and chemical composition of brown rice. Methods Twenty-two cannulated pigs (initial body weight: 42.0±1.2 kg) were assigned to a replicated 11×3 incomplete Latin square design, including an N-free diet and 10 brown rice diets. Each period included 5 d adaptation and 2 d ileal digesta collection. Chromic oxide was added at 0.3% to all the diets as an indigestible marker for calculating the ileal CP and AA digestibility. Results The coefficients of variation of all detected indices for physical characteristics and chemical composition, except for bulk weight, dry matter (DM) and gross energy, in 10 brown rice samples were greater than 10%. The SID of CP, lysine (Lys), methionine, threonine (Thr), and tryptophan (Trp) in brown rice was 77.2% (62.6% to 85.5%), 87.5% (80.3% to 94.3%), 89.2% (78.9% to 98.9%), 55.4% (46.1% to 67.6%) and 92.5% (86.3% to 96.3%), respectively. The best prediction equations for the SID of CP, Lys, Thr, and Trp were as following, SIDCP = −664.181+8.484×DM (R2 = 0.40), SIDLys = 53.126+6.031×ether extract (EE)+0.893×thousand-kernel volume (R2 = 0.66), SIDThr = 39.916+7.843×EE (R2 = 0.41), and SIDTrp = −361.588+4.891×DM+0.387×total starch (R2 = 0.85). Conclusion Overall, a great variation exists among 10 sources of brown rice, and the thousand-grain volume, DM, EE, and total starch can be used as the key predictors for SID of CP and AA.

Abstract Background Lupus nephritis (LN) is the most common cause of kidney injury in systemic lupus erythematosus (SLE) patients and is associated with increased mortality. DNA methylation, one of the most important epigenetic modifications, has been reported as a key player in the pathogenesis of SLE. Hence, our article aimed to explore DNA methylation in CD4+ T cells from LNs to identify additional potential biomarkers and pathogenic genes involved in the progression of LN. Methods Our study enrolled 46 SLE patients with or without kidney injury and 23 healthy controls from 2019 to 2022. CD4+ T cells were sorted for DNA methylation genotyping and RNA-seq. Through bioinformatics analysis, we identified the significant differentially methylated CpG positions (DMPs) only in the LN group and validated them by Bisulfite PCR. Integration analysis was used to screen for differentially methylated and expressed genes that might be involved in the progression of LN, and the results were analyzed via cell experiments and flow cytometry. Results We identified 243 hypomethylated sites and 778 hypermethylated sites only in the LN cohort. Three of these DMPs, cg08332381, cg03297029, and cg16797344, were validated by Bisulfite PCR and could be potential biomarkers for LN. Integrated analysis revealed that the expression of BCL2L14 and IFI27 was regulated by DNA methylation, which was validated by azacytidine (5-aza) treatment. The overexpression of BCL2L14 in CD4+ T cells might induce renal fibrosis and inflammation by regulating the differentiation and function of Tfh cells. Conclusion Our study identified novel aberrant DMPs in CD4+ T cells only in LN patients and DNA methylation-regulated genes that could be potential LN biomarkers. BCL2L14 is likely involved in the progression of LN and might be a treatment target.

Corn (C), wheat (W), and paddy rice (PR) are important energy sources and are commonly used in feed production for swine. This study mainly focuses on the variation and regularities of microbiota and metabolites in the gastrointestinal tract (GIT) of pigs in response to C, W, and PR. A total of 18 pigs were allotted into three dietary groups with six replicated pigs and received diets containing C, W, or PR as the sole energy source, respectively. The results showed that digestive parts significantly affected the diversity of microbial communities. Cereal grain sources significantly influenced the β-diversity of microbial communities in the colon and rectum. Campylobacterota and Proteobacteria are mainly distributed in the duodenum, Lactobacillus in the jejunum, and Bacteroidota in the colon and rectum. The W diet increased the Bacteroidota, Spirochaetota, and Prevotellaceae_NK3B31_group abundances and showed the highest concentrations of all short-chain fatty acids (SCFAs) in the hindgut. Fibrobacterota, Bacteroidota, Spirochaetota, Prevotellaceae_NK3B31_group, Prevotella, and Treponema in the colon or rectum were positively correlated with acetate, propionate, butyrate, and total SCFAs. These findings suggested that aerobic bacteria and facultative anaerobes in the foregut will gradually be replaced by anaerobes in the hindgut. The W diet had the best fermentability and was beneficial to the colonization of microbial communities that mainly used carbohydrates. The hindgut flora of the PR diet group may be more balanced with fewer potential pathogenic bacteria. Many microbial communities have been identified to contribute positively to the SCFA production of the hindgut. Collectively, our study revealed the spatial variation regularities of GIT microbial communities in an adult pig model and provided new insights into GIT microbiota and responses of metabolites to cereal grain diets.

Abstract Objective To explore the application effect of the direct reporting system of adverse nursing events and special continuous nursing quality improvement measures in the management of these adverse events. Methods The implementation time of continuous nursing improvement based on the direct reporting system was the demarcation point. We retrospectively collected and analyzed nursing adverse event reports and hospitalization data from Xiangtan Central Hospital before implementation (2015–2018) and after implementation (2019–2022). The active reporting rate of adverse events, the composition of these events and the processing time were compared between the two groups. Results The rate of active reporting of adverse events before the implementation was lower than that after the implementation (6.7% vs. 8.1%, X 2 = 25.561, P

Objective: The experiment was conducted to determine the apparent or standardized ileal digestibility (AID or SID) of crude protein (CP) and amino acids (AA) in 10 rapeseed cake samples fed to pigs, and to construct predictive models for the SID of CP and AA based on the chemical composition of rapeseed cakes. Methods: Twenty-two cannulated pigs (initial body weight: 39.8 ± 1.2 kg) were assigned to two 11 × 3 incomplete Latin square designs, including an N-free diet and 10 diets containing rapeseed cake. Each experimental period included 5 days of adaptation and 2 days of ileal digesta collection. Titanium dioxide (TiO2) was added at 0.3% to all the diets as an indigestible marker for calculating the ileal CP and AA digestibility. Results: The coefficients of variation (CV) of the content of crude fat (EE), crude fiber (CF), neutral detergent fiber (NDF), acid detergent fiber (ADF), and total glucosinolates (TGS) in 10 samples of rapeseed cake were greater than 10%. The standardized ileal digestibility (SID) of crude protein (CP), lysine (Lys), methionine (Met), threonine (Thr), and tryptophan (Trp) in rapeseed cake was 73.34% (61.49 to 81.12%), 63.01% (41.41 to 73.10%), 69.47% (50.55 to 88.16%), 79.61% (74.41 to 87.58%), and 94.43% (91.34 to 97.20%), respectively. The best prediction equations for SIDCP, SIDLys, and SIDVal were as follows: SIDCP = 90.124 − 0.54NDF (R2 = 0.58), SIDLys = 100.107 − 1.229NDF (R2 = 0.94), and SIDVal = 151.012 − 2.990TGS (R2 = 0.57). Conclusion: Overall, great variation exists among the 10 rapeseed cakes, and the NDF, TGS, and heating temperature can be used as the key predictors for the SID of CP and AA.

Sandy-clay mixtures are commonly used in civil engineering projects, such as transportation and water resources, due to their unique construction properties. While the macromechanical characteristics of these mixtures under saturated conditions are well understood, their behavior in unsaturated states is still not fully elucidated. This study aims to investigate the mechanical behavior of sand–clay mixtures under unsaturated conditions by examining the influence of varying sand content and matric suction on their shear strength. The research includes unsaturated shear strength tests and nuclear magnetic resonance (NMR) experiments to analyze the pore characteristics within the mixtures at different sand content levels. The correlation between changes in pore structure and shear strength is systematically analyzed. The unsaturated shear strength of the mixtures is predicted using the Fredlund shear strength formula and compared with the measured values. The results indicate that the unsaturated shear strength of the mixture decreases as the sand content increases but stabilizes once the sand content reaches 20%. Below 20% sand content, the changes in micropore distribution are not significant with increasing sand content. However, beyond 20% sand content, there is a noticeable increase in the ratio of large pores and a decrease in the proportion of small pores. The sand content of 20% represents a critical threshold for the strength and porosity variations in the mixture. Under constant matric suction, the unsaturated shear strength of the mixture improves with the increase in sand content. However, the predictive accuracy of the Fredlund shear strength formula gradually diminishes as the sand content increases.

ObjectiveTo evaluate the application of maternal and child health (MCH) handbook in Guangdong province for providing evidence to local promotion of MCH handbook utilization. MethodsUsing convenient sampling and a question-naire self-designed on the basis of reach, efficacy, adoption, implementation, and maintenance (RE-AIM) framework, we conducted an on-site on-line anonymous survey among 2 548 would-be mothers/pregnant women/puerpera visiting MCH institutions at 15 prefectures across Guangdong province and in-depth interviews among professionals responsible for issues about MCH handbook application in 6 MCH institutions at prefecture level during March – April 2021. ResultsOf the 2 384 valid participants completing the questionnaire survey, 82.16%, 67.72%, and 64.48% reported the awareness, registration, and utilization of MCH handbook. Not knowing details of the MCH handbook was reported by 25.58% of the participants being aware of the handbook but not having the registration for the handbook's utilization; not being aware of and not knowing application approach of the handbook were reported by 36.36% and 23.38% of the participants being registered holder of the handbook but not yet using the handbook. There were 92.50% of the participants being registered holder of the handbook completed their registration during pregnancy. Among the participants having used the handbook, 33.27% and 43.68% reported high and moderate satisfaction to the role of the handbook; 92.84% considered that the application of the handbook resulted in a desired effect; while, 33.46% expressed the worry for potential leakage of personal information. Among the participants having used the handbook and with maternal school involvement, 84.01% reported recognizing the significance of the MCH handbook by taking part in programs of maternal school. Impaired and delayed information sharing associated with the handbook utilization among the MCH institutions were reported by professionals undertaking in-depth interviews. In all the MCH handbook registrants, 58.65% were registered for the application of the handbook of both paper and electronic versions. Regional disparity was revealed in responsibilities assigned to the MCH institutions registering the handbook holders. Not knowing application approach of the handbook were reported by 10.09% of the participants having used the handbook. In terms of maintenance in application of the handbook, reading through the handbook, completing all self-documenting content in the handbook, and applying the handbook two times or more in a week were reported by 24.54%, 18.82%, and 36.33% of the participants having used handbook. Impaired interconnection in the information system for MCH handbook application was indicated by the professionals interviewed. ConclusionThe efficacy of MCH handbook application is good in Guangdong province, but publicity of the handbook, unification of MCH institutions′ responsibilities and optimization of information system for MCH handbook utilization still need to be improved. Meanwhile, it is necessary to explore the integration mechanism with maternal systematic management and children systematic management in basic public health service projects.

In recent years, rehabilitation robots have been developed and used in rehabilitation training for patients with hemiplegia. In this paper, a rehabilitation training robot with variable damping is designed to train patients with hemiplegia to recover upper limb function. Firstly, a magnetorheological joint damper (MR joint damper) is designed for the rehabilitation training robot, and its structural design and dynamic model are tested theoretically and experimentally. Secondly, the rehabilitation robot is simplified into a spring-damping system, and the rehabilitation training controller for human movement is designed. The rehabilitation robot dynamically adjusts the excitation current according to the feedback speed and human–machine interaction torque, so that the rehabilitation robot always outputs a stable torque. The magnetorheological joint damper acts as a clutch to transmit torque safely and stably to the robot joint. Finally, the upper limb rehabilitation device is tested. The expected torque is set to 20 N, and the average value of the output expected torque during operation is 20.02 N, and the standard deviation is 0.635 N. The output torque has good stability. A fast (0.5 s) response can be achieved in response to a sudden motor speed change, and the average expected output torque is 20.38 N and the standard deviation is 0.645 N, which can still maintain the stability of the output torque.

This study proposes a dual-coil magnetorheological torsional vibration damper (MRTVD) and verifies the effectiveness of semi-active damping control to suppress the shaft system’s torsional vibration via experimental research. Firstly, the mechanical model of the designed MRTVD and its coupling mechanical model with the rotating shaft system are established. Secondly, the torsional response of the shaft system is obtained via resonance experiments, and the influence of the current on the torsional characteristics of the magnetorheological torsional damper is analyzed. Finally, the MRTVD is controlled using the skyhook control approach. The experimental results demonstrate that when the main shaft passes through the critical speed range at various accelerations, the amplitude of the shaft’s torsional vibration decreases by more than 15%, and the amplitude of the shaft’s torsional angular acceleration decreases by more than 22%. These conclusions validate the inhibitory effect of MRTVD on the main shaft’s torsional vibrations under skyhook control.

The Xiong’an New Area, following the precedent of the Shenzhen Special Economic Zone and Shanghai Pudong New Area, marks a significant development. This study introduces a method to optimize the feature variable selection for Sentinel-2 images from 2016 to 2022, aiming for precise land-use classification in Xiong’an using machine learning. The classification reveals substantial growth in the infrastructure and aquatic areas in Rongcheng and Xiongxian counties, outpacing Anxin from 2016 to 2022. The Remote Sensing-Based Ecological Index (RSEI) indicates a generally stable yet improving ecological landscape, especially in denser areas like Xiongxian and Rongcheng, aligning regional development with ecological enhancement. EOF analysis shows a spatial ecological division, with positive RSEI values in the western regions and negative values in the east, along with temporal fluctuations indicating a decline in the west and an increase in the east since 2017. Additionally, the RSEI’s short-cycle fluctuations emphasize the dynamic ecological state of the area, influenced by both long-term trends and transient factors.

Cardiovascular diseases rank as the leading cause of death worldwide and are a major contributor to disability, posing a significant threat to human health. Organoids offer a partial simulation of the structure and function of the tissue of origin. It is a promising model that can supplement the disadvantages of two-dimensional culture and animal models. Due to the complexity of heart development, the research of cardiac organoids is still maturing. The advancement of technology has helped address certain challenges, but it has also unveiled new issues and complexities. This paper summarizes the application of organoids technology in the cardiovascular field, the common construction methods of cardiac organoids, and the latest progress of cardiac organoids in the fields of disease model construction, cardiac development research, drug research, and regenerative medicine. The future development and challenges of cardiac organoids are also addressed.

Introduction: The COVID-19 global pandemic is far from ending. There is an urgent need to identify applicable biomarkers for early predicting the outcome of COVID-19. Growing evidences have revealed that SARS-CoV-2 specific antibodies evolved with disease progression and severity in COIVD-19 patients. Objectives: We assumed that antibodies may serve as biomarkers for predicting the clinical outcome of hospitalized COVID-19 patients on admission. Methods: By taking advantage of a newly developed SARS-CoV-2 proteome microarray, we surveyed IgG responses against 20 proteins of SARS-CoV-2 in 1034 hospitalized COVID-19 patients on admission and followed till 66 days. The microarray results were further correlated with clinical information, laboratory test results and patient outcomes. Cox proportional hazards model was used to explore the association between SARS-CoV-2 specific antibodies and COVID-19 mortality. Results: Nonsurvivors (n = 955) induced higher levels of IgG responses against most of non-structural proteins than survivors (n = 79) on admission. In particular, the magnitude of IgG antibodies against 8 non-structural proteins (NSP1, NSP4, NSP7, NSP8, NSP9, NSP10, RdRp, and NSP14) and 2 accessory proteins (ORF3b and ORF9b) possessed significant predictive power for patient death, even after further adjustments for demographics, comorbidities, and common laboratory biomarkers for disease severity (all with p trend

BackgroundPrevious studies showed conflicting evidence on the association between the intake of dietary branched-chain amino acid (BCAA) and the risk of cardiovascular disease (CVD). However, this relationship has not been studied in patients with type 2 diabetes. Therefore, we evaluated the effects of total and individual dietary BCAA (leucine, isoleucine, and valine) intake on CVD risk among individuals with type 2 diabetes in China.Materials and methodsA total of 419 patients with type 2 diabetes who have been diagnosed with CVD (within 2 weeks) were recruited between March 2013 and September 2015 in China. Cases with CVD were 1:1 matched to controls with type 2 diabetes but without CVD by age (±5 years) and sex. A validated 79-item semiquantitative food frequency questionnaire (FFQ) was administered to assess the participants' dietary data. Total dietary BCAA per individual was the summation of the daily intake of isoleucine, leucine, and valine. OR and corresponding CIs were computed by conditional logistic regression models adjusted for potential confounders.ResultsMedian values of the daily intake of total BCAA were 11.87 g, with an interquartile range of 10.46–13.15 g for cases, and 12.47 g, with an interquartile range of 11.08–13.79 g for controls (P = 0.001). Dietary BCAA was inversely related to CVD risk after multivariable adjustment (OR Q4−Q1 = 0.23, 95%CI = 0.10, 0.51, P trend

Gram-positive Streptomyces bacteria can produce valuable secondary metabolites. Streptomyces genomes include huge unknown silent natural product (NP) biosynthetic gene clusters (BGCs), making them a potential drug discovery repository. To collect antibiotic-producing bacteria from unexplored areas, we identified Streptomyces sp. CS-7 from mountain soil samples in Changsha, P.R. China, which showed strong antibacterial activity. Complete genome sequencing and prediction in silico revealed that its 8.4 Mbp genome contains a total of 36 BGCs for NPs. We purified two important antibiotics from this strain, which were structurally elucidated to be mayamycin and mayamycin B active against Staphylococcus aureus. We identified functionally a BGC for the biosynthesis of these two compounds by BGC direct cloning and heterologous expression in Streptomyces albus. The data here supported this Streptomyces species, especially from unexplored habitats, having a high potential for new NPs.

BackgroundLupus nephritis (LN) is one of the most common and serious complications of systemic lupus erythaematosus (SLE). Genetic factors play important roles in the pathogenesis of LN and could be used to predict who might develop LN. The purpose of this study was to screen for susceptible candidates of LN across the whole genome in the Han Chinese population.Methods592 LN patients and 453 SLE patients without renal damage were genotyped at 492,970 single nucleotide polymorphisms (SNPs) in the genome-wide association study (GWAS). Fifty-six SNPs were selected for replication in an independent cohort of 188 LN and 171 SLE without LN patients. Further quantitative real-time (qRT) PCR was carried out in 6 LN patients and 6 healthy controls. Gene-based analysis was conducted using the versatile gene-based test for GWAS. Subsequently, enrichment and pathway analyses were performed in the DAVID database.ResultsThe GWAS analysis and the following replication research identified 9 SNPs showing suggestive correlation with LN (P

The purpose of this study was to solve the problem that the damping of rubber or silicone oil torsional dampers used in crankshafts is not adjustable and cannot effectively control torsional vibration at different resonant frequencies. Based on the controlled rheological properties of magnetorheological (MR) smart materials, this study designed a new type of variable damping MR torsional damper (MRTD) and proposed a semi-active control method to effectively control the torsional vibration of the crankshaft under multiple harmonic resonances. First, a mechanical model of the MRTD and a lumped parametric mass model of the crankshaft system were developed, and the resonance frequency harmonic range of the crankshaft system operation was determined by the torsional vibration characteristics analysis. Then a semi-active skyhook control method for the MRTD was proposed, and a joint control simulation analysis was performed using Amesim and Matlab software. The torsional vibration control effects of the crankshaft system with no damper, MRTD with different damping coefficients, and MRTD with skyhook control under acceleration and uniform speed conditions were comprehensively investigated. The simulation results indicated that the skyhook damping control significantly reduced the torsional vibration amplitude under both acceleration and uniform speed conditions, verifying the effectiveness of the skyhook-based control strategy for MRTD.

Shock loads can pose a great threat to personnel or instruments, and efficient control of the buffering process is an effective means of reducing damage from shock energy. In this paper, magneto-rheological grease was used as the internal controllable material of the buffer to address the turbulence and settling problems of conventional magneto-rheological fluid. A bending and folding back magnetic circuit is proposed, and the magnetic circuit simulation was verified. The corresponding dynamic mechanical model was established, and the mechanical response characteristics of the buffer under impact load were also simulated dynamically. The mechanical properties of the designed and processed device were tested, and a variable current control method was used to improve the performance of the shock resistance of the buffer. The response of the magnetorheological grease buffer under different drop hammer impacts was investigated. The buffering effect and controllability of the buffer were analyzed by comparing the acceleration, velocity, and top-end cap displacement at the same drop hammer height for different current magnitudes. The results show that the buffer performance of the buffer gradually improved as the current increased. The response time of the designed new magnetorheological buffer was determined by the jump time of the peak damping force to be 9 ms. Lastly, the controllability was verified by manually and automatically adjusting the current magnitude, and the results were compared with those at 300 mm drop hammer height and 0.5 A current magnitude, and the continuous variable current control was found to be effective. This provides a feasible reference for scholars to study optimal buffer control.

To reveal the transient temperature distribution pattern inside the magnetorheological grease (MRG) torsional vibration damper and explore the relationship between the current and internal temperature of the device, the transient temperature simulation analysis of the MRG device was conducted in this study. Firstly, a theoretical heat transfer model of MRG torsional vibration damper with dual heat source structural feature was established based on the Bingham constitutive model and the temperature-dependent viscosity characteristic of MRG. Then, the transient temperature field model of the MRG torsional vibration damper was developed by the finite element method, the temperature field distribution and temperature–time variation characteristics of the MRG torsional vibration damper at 0A, 1A, and 2A working conditions were analyzed, and the effects of viscosity and slip factors on the temperature rise of the device were investigated. The simulation results show that the temperature rise of MRG in the working domain is the fastest, but a gradual slowing of the temperature rise rate. The magnitude and rate of temperature rise are maximum when the 1A current is applied to the torsional vibration damper. Finally, the current–temperature curve is obtained by fitting the simulation results. The results of the analysis reveal the internal temperature distribution and temperature rise characteristics of the torsional vibration damper, which provide a theoretical basis for the structural optimization and control strategy design of the MRG torsional vibration damper considering temperature as a factor.

ObjectivesTo quantify the burden and variation trends of cancers in children under 5 years at the global, regional, and national levels from 1990 to 2019.MethodsEpidemiological data for children under 5 years who were diagnosed with any one childhood cancer were obtained from the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) from 1990 to 2019. The outcomes were the absolute numbers and rates of incidence, prevalence, mortality, and disability-adjusted life-years (DALYs) for different types of cancer.ResultsIn 2019, 8,774,979.1 incident cases (95% uncertainty interval [UI]: 6,243,599.2 to11,737,568.5) and 8,956,583.8 (6,446,323.9 to 12,364,520.8) prevalent cases of cancer in children under 5 years were identified worldwide; these cancers resulted in 44,451.6 (36,198.7 to 53,905.9) deaths and 3,918,014.8 (3,196,454.9 to 4,751,304.2) DALYs. From 1990 to 2019, although the numbers of incident and prevalent cases only decreased by −4.6% (−7.0 to −2.2) and −8.3% (−12.6 to −3.4), respectively, the numbers of deaths and DALYs clearly declined by −47.8% (−60.7 to −26.4) and −47.7% (−60.7 to −26.2), respectively. In 2019, the middle sociodemographic index (SDI) regions had the highest incidence and prevalence, whereas the low SDI regions had the most mortality and DALYs. Although all of the SDI regions displayed a steady drop in deaths and DALYs between 1990 and 2019, the low-middle and low SDI regions showed increasing trends of incidence and prevalence. Leukemia remained the most common cancer globally in 2019. From 1990 to 2019, the burdens of leukemia, liver cancer, and Hodgkin's lymphoma declined, whereas the incidence and prevalence of other cancers grew, particularly testicular cancer.ConclusionsThe global childhood cancer burden in young children has been steadily decreasing over the past three decades. However, the burdens and other characteristics have varied across different regions and types of cancers. This highlights the need to reorient current treatment strategies and establish effective prevention methods to reduce the global burden of childhood cancer.

The purpose of this study is to provide a convenient optimization design method for magnetorheological torsional vibration absorbers (MR-TVA) suitable for automotive engines, which is a damper matching design method that takes into account the needs of the engine operating conditions. In this study, three kinds of MR-TVA with certain characteristics and applicability are proposed: axial single-coil configuration, axial multi-coil configuration and circumferential configuration. The magnetic circuit model, damping torque model and response time model of MR-TVA are established. Then, under the constraints of weight, size and inertia ratio, according to different torsional vibration conditions, the MR-TVA mass, damping torque and response time are multi-objective optimized in two directions. The optimal configurations of the three configurations are obtained from the intersection of the two optimal solutions, and the performance of the optimized MR-TVA is compared and analyzed. The results show that the axial multi-coil structure has large damping torque and the shortest response time (140 ms), which is suitable for complex working conditions. The damping torque of the axial single coil structure is generally large (207.05 N.m), which is suitable for heavy load conditions. The circumferential structure has a minimum mass (11.03 kg) and is suitable for light load conditions.

The growing world population and increasing life expectancy are driving the need to improve the quality of blood transfusion, organ transplantation, and preservation. Here, to improve the ability of red blood cells (RBCs) for normothermic machine perfusion, a biocompatible blood silicification approach termed "shielding-augmenting RBC-in-nanoscale amorphous silica (SARNAS)" has been developed. The key to RBC surface engineering and structure augmentation is the precise control of the hydrolysis form of silicic acid to realize stabilization of RBC within conformal nanoscale silica-based exoskeletons. The formed silicified RBCs (Si-RBCs) maintain membrane/structural integrity, normal cellular functions (e.g., metabolism, oxygen-carrying capability), and enhance resistance to external stressors as well as tunable mechanical properties, resulting in nearly 100% RBC cryoprotection. In vivo experiments confirm their excellent biocompatibility. By shielding RBC surface antigens, the Si-RBCs provide universal blood compatibility, the ability for allogeneic mechanical perfusion, and more importantly, the possibility for cross-species transfusion. Being simple, reliable, and easily scalable, the SARNAS strategy holds great promise to revolutionize the use of engineered blood for future clinical applications. [ABSTRACT FROM AUTHOR]

Abstract There is a dearth of effective parameters for selecting potentially transplantable liver grafts from expanded-criteria donors. In this study, we used a nuclear magnetic resonance (NMR) relaxation analyzer-based assay to assess the viability of ex vivo livers obtained via porcine donation after circulatory death (DCD). Ex situ normothermic machine perfusion (NMP) was utilized as a platform for viability test of porcine DCD donor livers. A liver-targeted contrast agent, gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA), was injected into the perfusate during NMP, and the dynamic biliary excretion of the Gd-EOB-DTPA was monitored by measuring the longitudinal relaxation time (T1). The longitudinal relaxation rate (R1) of the bile was served as a parameter. The delay of increase in biliary R1 during early stage of NMP indicated the impaired function of liver grafts in both warm and cold ischemia injury, which was correlated with the change of alanine aminotransferase. The preservative superiority in cold ischemia of dual hypothermic oxygenated machine perfusion could also be verified by assessing biliary R1 and other biochemical parameters. This study allows for the dynamic assessment of the viability of porcine DCD donor livers by combined usage of ex situ NMP and NMR relaxation time based assay, which lays a foundation for further clinical application.

Compared with the central nervous system, the adult peripheral nervous system possesses a remarkable regenerative capacity, which is due to the strong plasticity of Schwann cells (SCs) in peripheral nerves. After peripheral nervous injury, SCs de-differentiate and transform into repair phenotypes, and play a critical role in axonal regeneration, myelin formation, and clearance of axonal and myelin debris. In view of the limited self-repair capability of SCs for long segment defects of peripheral nerve defects, it is of great clinical value to supplement SCs in necrotic areas through gene modification or stem cell transplantation or to construct tissue-engineered nerve combined with bioactive scaffolds to repair such tissue defects. Based on the developmental lineage of SCs and the gene regulation network after peripheral nerve injury (PNI), this review summarizes the possibility of using SCs constructed by the latest gene modification technology to repair PNI. The therapeutic effects of tissue-engineered nerve constructed by materials combined with Schwann cells resembles autologous transplantation, which is the gold standard for PNI repair. Therefore, this review generalizes the research progress of biomaterials combined with Schwann cells for PNI repair. Based on the difficulty of donor sources, this review also discusses the potential of “unlimited” provision of pluripotent stem cells capable of directing differentiation or transforming existing somatic cells into induced SCs. The summary of these concepts and therapeutic strategies makes it possible for SCs to be used more effectively in the repair of PNI.

The conventional pneumatic soft robot has the problem of insufficient stiffness, while in the magnetorheological soft robot, the magnetic field provided by electromagnet has the disadvantage of oversized structure and poor flexibility. This paper presents a variable stiffness pneumatic soft robot based on magnetorheological grease (MRG) to solve these problems. Its three soft fingers cooperate with the adjustable gripper to adjust the gripping range for the robot hand, and it is used to provide gripping driving force through the bending drive. The MRG layer is designed on the gripping surface to provide adaptivity and rigid support for the gripped objects. A magnetic-air structure consisting of a Halbach array and Halbach array actuator is designed inside the soft fingers to provide a flexible magnetic field for the MRG layer. Theoretical and simulation analysis is carried out, and the results show that the state of the MRG changes and the stiffness of the clamping surface changes under the working pressure of 30 kPa. Finally, the experiment further proves the variable and high adaptivity of the surface stiffness of the gripping surface to reduce the damage to the gripped objects.

In order to identify the nonlinear characteristics of the magnetorheological (MR) damper applied in multi-DOF vibration reduction platforms in the aerospace field in the modeling process, the least square support vector machine (LS-SVM) method is adopted, because LS-SVM can handle small-sample, high-dimensional characteristic problems. Firstly, the theory of the modeling method based on LS-SVM was illustrated including the genetic algorithm (GA) optimization method. Secondly, the characteristic curve of the MR damper was tested based on different conditions. Then, the current and historical input displacement, velocity, and current and the historical output are taken as the input of the LS-SVM model and the damping force of the current output is taken as the output of the model for model training. Meanwhile, the genetic algorithm is introduced to optimize the parameters of the LS-SVM model which affect the accuracy of the model, the penalty factor c=16.48, and the kernel parameter σ=3.39 after optimization. Finally, in order to verify the method adopted in the paper, the Simulink model was simulated in certain input conditions; by comparing the simulation and experimental values of this model, it is found that the maximum error is within 10 N and the average error is around 0.89 N, which is similar to the accuracy obtained in other works of literature, and the correctness of this model is verified.

Magnetorheological (MR) absorbers in the artillery recoil systems are usually used to dissipate the impact energy as much as possible and reduce the recoil force transmitted to the artillery carriage, while the firing stability of artillery during the buffer process is rarely considered. In this paper, we analyzed the firing stability characteristics of the fixed artillery and the field artillery systems and established corresponding mechanical models. Then, we proposed the ideal recoil F–v curves of these two kinds of artillery, respectively. The “platform effect” of recoil curve was taken as the recoil force control target of the fixed artillery, while based on the firing stability, the linear segmented recoil curve was drawn up as the ideal recoil buffer control target of the field artillery. To verify the feasibility and controllability of the designed multi-stage MR absorber in two kinds of recoil buffer system, the impact tests were conducted under different current loadings. The test results show that the designed MR absorber can realize different buffer control effects by changing the input current, but the ideal “platform effect” recoil curve of the fixed artillery cannot be completely realized due to small controllable damping force output. In the field artillery recoil system, the MR absorber can realize ideal recoil buffer control in the range of 0°–25° firing angles.

Luminal pH is an important functional feature of intracellular organelles. Acidification of the lumen of organelles such as endosomes, lysosomes, and the Golgi apparatus plays a critical role in fundamental cellular processes. As such, measurement of the luminal pH of these organelles has relevance to both basic research and translational research. At the same time, accurate measurement of intraorganellar pH in living cells can be challenging and may be a limiting hurdle for research in some areas. Here, we describe three powerful methods to measure rigorously the luminal pH of different intracellular organelles, focusing on endosomes, lysosomes, and the Golgi apparatus. The described methods are based on live imaging of pH-sensitive fluorescent probes and include: (1) A protocol based on quantitative, ratiometric measurement of endocytosis of pH-sensitive and pH-insensitive fluorescent conjugates of transferrin; (2) A protocol for the use of proteins tagged with a ratiometric variant of the pH-sensitive intrinsically fluorescent protein pHluorin; and (3) A protocol using the fluorescent dye LysoSensor™. We describe necessary reagents, key procedures, and methods and equipment for data acquisition and analysis. Examples of implementation of the protocols are provided for cultured cells derived from a cancer cell line and for primary cultures of mouse hippocampal neurons. In addition, we present strengths and weaknesses of the different described intraorganellar pH measurement methods. These protocols are likely to be of benefit to many researchers, from basic scientists to those conducting translational research with a focus on diseases in patient-derived cells.

The recent discovery of hepatitis E virus (HEV) strains in rabbits in the People’s Republic of China and the United States revealed that rabbits are another noteworthy reservoir of HEV. However, whether HEV from rabbits can infect humans is unclear. To study the zoonotic potential for and pathogenesis of rabbit HEV, we infected 2 cynomolgus macaques and 2 rabbits with an HEV strain from rabbits in China. Typical hepatitis developed in both monkeys; they exhibited elevated liver enzymes, viremia, virus shedding in fecal specimens, and seroconversion. Comparison of the complete genome sequence of HEV passed in the macaques with that of the inoculum showed 99.8% nucleotide identity. Rabbit HEV RNA (positive- and negative-stranded) was detectable in various tissues from the experimentally infected rabbits, indicating that extrahepatic replication may be common. Thus, HEV is transmissible from rabbits to cynomolgus macaques, which suggests that rabbits may be a new source of human HEV infection.