33 results on '"Qi, Jun-Shan"'
Search Results
2. Effects of permanent left bundle branch area pacing on QRS duration and short-term cardiac function in pacing-indicated patients with left bundle branch block
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Qi-Jun Shan, Hai Xu, Xiu-Juan Zhou, Qing Chang, Ling Ji, Chun Chen, Zhi-Xin Jiang, and Ning-Ning Wang
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Medicine - Published
- 2021
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3. Intestinal Barrier Dysfunction Exacerbates Neuroinflammation via the TLR4 Pathway in Mice With Heart Failure
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Jun-Yu Huo, Wan-Ying Jiang, Ting Yin, Hai Xu, Yi-Ting Lyu, Yuan-Yuan Chen, Meng Chen, Jie Geng, Zhi-Xin Jiang, and Qi-Jun Shan
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heart failure ,neuroinflammation ,intestinal barrier ,lipopolysaccharide ,TLR4 ,Physiology ,QP1-981 - Abstract
AimsThe present study aimed to investigate alterations in neuroinflammation after heart failure (HF) and explore the potential mechanisms.MethodsMale wild-type (WT) and Toll-like receptor 4 (TLR4)-knockout (KO) mice were subjected to sham operation or ligation of the left anterior descending coronary artery to induce HF. 8 weeks later, cardiac functions were analyzed by echocardiography, and intestinal barrier functions were examined by measuring tight junction protein expression, intestinal permeability and plasma metabolite levels. Alterations in neuroinflammation in the brain were examined by measuring microglial activation, inflammatory cytokine levels and the proinflammatory signaling pathway. The intestinal barrier protector intestinal alkaline phosphatase (IAP) and intestinal homeostasis inhibitor L-phenylalanine (L-Phe) were used to examine the relationship between intestinal barrier dysfunction and neuroinflammation in mice with HF.ResultsEight weeks later, WT mice with HF displayed obvious increases in intestinal permeability and plasma lipopolysaccharide (LPS) levels, which were accompanied by elevated expression of TLR4 in the brain and enhanced neuroinflammation. Treatment with the intestinal barrier protector IAP significantly attenuated neuroinflammation after HF while effectively increasing plasma LPS levels. TLR4-KO mice showed significant improvements in HF-induced neuroinflammation, which was not markedly affected by intestinal barrier inhibitors or protectors.ConclusionHF could induce intestinal barrier dysfunction and increase gut-to-blood translocation of LPS, which could further promote neuroinflammation through the TLR4 pathway.
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- 2021
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4. Renal Denervation Attenuates Neuroinflammation in the Brain by Regulating Gut-Brain Axis in Rats With Myocardial Infarction
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Jun-Yu Huo, Wan-Ying Jiang, Yi-Ting Lyu, Lin Zhu, Hui-Hui Liu, Yuan-Yuan Chen, Meng Chen, Jie Geng, Zhi-Xin Jiang, and Qi-Jun Shan
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renal denervation ,myocardial infarction ,neuroinflammation ,intestinal injury ,gut microbiota ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Aims: The development of neuroinflammation deteriorates the prognosis of myocardial infarction (MI). We aimed to investigate the effect of renal denervation (RDN) on post-MI neuroinflammation in rats and the related mechanisms.Methods and Results: Male adult Sprague-Dawley rats were subjected to sham or ligation of the left anterior descending coronary artery to induce MI. One week later, the MI rats received a sham or RDN procedure. Their cardiac functions were analyzed by echocardiography, and their intestinal structures, permeability, and inflammatory cytokines were tested. The intestinal microbiota were characterized by 16S rDNA sequencing. The degrees of neuroinflammation in the brains of rats were analyzed for microglia activation, inflammatory cytokines, and inflammation-related signal pathways. In comparison with the Control rats, the MI rats exhibited impaired cardiac functions, intestinal injury, increased intestinal barrier permeability, and microbial dysbiosis, accompanied by increased microglia activation and pro-inflammatory cytokine levels in the brain. A RDN procedure dramatically decreased the levels of renal and intestinal sympathetic nerve activity, improved cardiac functions, and mitigated the MI-related intestinal injury and neuroinflammation in the brain of MI rats. Interestingly, the RDN procedure mitigated the MI-increased intestinal barrier permeability and pro-inflammatory cytokines and plasma LPS as well as ameliorated the gut microbial dysbiosis in MI rats. The protective effect of RDN was not significantly affected by treatment with intestinal alkaline phosphatase but significantly reduced by L-phenylalanine treatment in MI rats.Conclusions: RDN attenuated the neuroinflammation in the brain of MI rats, associated with mitigating the MI-related intestinal injury.
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- 2021
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5. Trimethylamine N-oxide facilitates the progression of atrial fibrillation in rats with type 2 diabetes by aggravating cardiac inflammation and connexin remodeling
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Wan-Ying Jiang, Jun-Yu Huo, Sheng-Chan Wang, Yan-Di Cheng, Yi-Ting Lyu, Zhi-Xin Jiang, and Qi-Jun Shan
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Male ,Inflammation ,Methylamines ,Diabetes Mellitus, Type 2 ,Physiology ,Atrial Fibrillation ,Animals ,Atrial Remodeling ,General Medicine ,Biochemistry ,Connexins ,Rats ,Diabetes Mellitus, Experimental - Abstract
Diabetes is an independent risk factor for atrial fibrillation (AF). This study aimed to elucidate the pathophysiology of diabetes-related AF from the perspective of the gut microbial metabolite trimethylamine N-oxide (TMAO). In the present study, male rats received either a normal diet to serve as the control group or a high-fat diet/streptozotocin to induce type 2 diabetes mellitus. Then, diabetic rats were divided into two groups based on the presence or absence of 3,3-dimethyl-1-butanol (DMB, a specific TMAO inhibitor) in drinking water: the diabetic cardiomyopathy (DCM) group and the DCM + DMB group. Eight weeks later, compared with control rats, rats in the DCM group exhibited gut microbiota dysbiosis and systemic TMAO elevation. The inflammatory cytokines IL-1β, IL-6, and TNF-α were markedly increased in the atria of rats in the DCM group. Downregulated expression of connexin 40 and lateralized distribution of connexin 43 were also observed in the atria of DCM rats. AF inducibility was significantly higher in DCM rats than in control rats. Furthermore, DMB treatment effectively ameliorated atrial inflammation and connexin remodeling while markedly reducing plasma TMAO levels. DMB treatment also decreased the vulnerability of diabetic rats to AF. In conclusion, TMAO might promote atrial inflammation and connexin remodeling in the development of diabetes, which may play a key role in mediating diabetes-related AF.
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- 2022
6. Renal denervation ameliorates post-infarction cardiac remodeling in rats through dual regulation of oxidative stress in the heart and brain
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Wan-Ying Jiang, Jun-Yu Huo, Chu Chen, Ran Chen, Tian-Tian Ge, Qing Chang, Jing-Wen Hu, Jie Geng, Zhi-Xin Jiang, and Qi-Jun Shan
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Renal denervation ,Angiotensin receptor neprilysin inhibitors ,Cardiac remodeling ,Oxidative stress ,Hypothalamus ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Myocardial remodeling is the key step in the development of ischemic cardiomyopathy. We aimed to compare effects of renal denervation (RDN) with those of angiotensin receptor neprilysin inhibitors (ARNi) on cardiac remodeling after myocardial infarction (MI), and explore underlying mechanism. Methods: Sprague-Dawley rats (n = 40; male) were subjected to ligation of left anterior descending coronary artery to induce MI; six rats served as controls. ARNi was administered at a dose of 60 mg/kg/day for 4 weeks starting 1 week after MI. An RDN/Sham-RDN procedure was performed 1 week after MI. Rats in all groups were studied 5 weeks after MI. Echocardiography was used to evaluate cardiac function. Masson staining and TUNEL staining were used to determine the extent of cardiac remodeling. Indicators of oxidative stress in heart and brain were used to analyze the potential mechanisms involved. Results: Five weeks after MI, both RDN and ARNi significantly improved cardiac function and cardiac remodeling; however, RDN was superior to ARNi at attenuating myocardial apoptosis. Compared to ARNi, RDN was also more effective at decreasing the abnormal oxidative stress caused by MI; this was especially true in case of the brain and was confirmed by evaluating the changes in reactive oxygen species (ROS) levels and other oxidative stress parameters following MI. Conclusions: RDN is not inferior to ARNi with respect to the improvement of cardiac remodeling in rats with ischemic cardiomyopathy. The effect of RDN might be associated with effective inhibition of oxidative stress in both the heart and brain.
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- 2019
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7. The effect of graphite oxide on the thermoelectric properties of polyaniline
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Zhao, Yuan, Tang, Guang-Shi, Yu, Zhong-Zhen, and Qi, Jun-Shan
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- 2012
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8. Dendrographium multiseptatum sp. nov. from China
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Ma, Li-Guo, primary, Zhang, Yue-Li, additional, Zhang, Bo, additional, Qi, Kai, additional, Li, Chang-Song, additional, and Qi, Jun-Shan, additional
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- 2020
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9. Meta-Analysis of Efficacy and Safety of Apixaban in Patients Undergoing Catheter Ablation for Atrial Fibrillation
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Dasheng, Lu, Qian, Liu, Kai, Wang, Q I, Zhang, and Qi-Jun, Shan
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Male ,Pyridones ,Incidence ,Comorbidity ,Middle Aged ,Postoperative Hemorrhage ,Causality ,Treatment Outcome ,Fibrinolytic Agents ,Risk Factors ,Thromboembolism ,Atrial Fibrillation ,Catheter Ablation ,Humans ,Pyrazoles ,Female ,Aged - Abstract
The efficacy and safety of apixaban in patients undergoing catheter ablation (CA) for atrial fibrillation (AF) are little investigated.The Cochrane Central Register of Controlled Trials (CENTRAL), MEDLINE, and EMBASE were searched up to September 2015. Four literatures comparing apixaban with vitamin K antagonists (VKAs) were included. Data were pooled in Review Manager Software, using Mantel-Haenszel methods with a fixed-effects model. The funnel plots and Egger's test were used to examine publication bias. Heterogeneity was assessed using the I(2) test. Risk ratios (RR) and 95% confidence intervals (CI) of each study were calculated and pooled.No significant differences were observed in rates of total bleeding (RR = 0.91, 95% CI [0.57, 1.46], I(2) = 0.0%), thromboembolic complications (RR = 0.75, 95% CI [0.03, 18.22], I(2) = 0.0%), or total events (RR = 0.90, 95% CI [0.56, 1.44], I(2) = 0.0%) between apixaban and VKAs group. The frequency of major bleeding was similar between apixaban and VKAs group (RR = 1.34, 95% CI [0.34, 5.30], I(2) = 0.0%).Apixaban was as effective and safe as VKAs in the periprocedural period of CA.
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- 2015
10. [Catheter ablation of epicardial accessory pathway associated with coronary sinus musculature]
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Wei-zhu, Ju, Ming-long, Chen, Bing, Yang, Feng-xiang, Zhang, Hong-wu, Chen, Jin-bo, Yu, Kai, Gu, Qi-jun, Shan, Jian-gang, Zou, Chun, Chen, Xiao-feng, Hou, and Ke-jiang, Cao
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Adult ,Male ,Young Adult ,Catheter Ablation ,Coronary Sinus ,Humans ,Female ,Middle Aged ,Pericardium ,Follow-Up Studies ,Retrospective Studies - Abstract
To report the single-center clinical experience of catheter ablation of epicardial accessory pathway associated with coronary sinus musculature.The data of 721 cases of left sided accessory pathway ablation were retrospectively analyzed. Ablation in the coronary sinus was performed in 17 (2.4 %) cases [11 males, mean age (37 ± 11) years].Among the 17 cases, the accessory pathway was successfully ablated in middle cardiac vein and posterior lateral coronary sinus in 11 and 6 cases, respectively. Deverticulum of middle cardiac vein was seen in 2 cases. Mean time required to block the accessory pathway was (4.7 ± 2.7) s. An accessory pathway potential could be recorded at the target site in 10 out of 17 patients (59%). During a mean (21 ± 16) months follow up, only one patient experienced recurrence who was successfully cured by a second ablation session. No procedure related complication was reported.About 2.4% of left accessory pathway may have epicardial connection locating at middle cardiac vein or lateral part of the coronary sinus and require epicardial ablation. The epicardial ablation is safe and effective, warrants an excellent long-term results.
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- 2013
11. [Long-term outcomes and factors for predicting ventricular arrhythmia in patients with catecholaminergic polymorphic ventricular tachycardia]
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Sheng-Yong, Yu, Jian-Hua, Shen, Jing-Ping, Lu, Dong-Sheng, Zhao, Gang, Zhang, Jie, Geng, and Qi-Jun, Shan
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Adult ,Male ,Electrocardiography ,Treatment Outcome ,Adolescent ,Adrenergic beta-Antagonists ,Electrocardiography, Ambulatory ,Tachycardia, Ventricular ,Humans ,Female ,Child - Abstract
To evaluate factors for predicting ventricular arrhythmia, the clinical effect of drugs on patients with catecholaminergic polymorphic ventricular tachycardia (CPVT), and their long-term outcomes.Six patients diagnosed with CPVT underwent a series of electrocardiograms and 24-hour Holter monitoring. β-blockers were recommended for all patients, while some patients were also prescribed propafenone and 1 patient underwent catheter-based renal sympathetic denervation (RDN). The characteristics of electrocardiogram, arrhythmia and long-term outcomes were monitored.Syncope episodes did not occur any longer in 1 patient on β-blocker, but recurred in 3 other patients and 2 patients died (one due to his cessation of metoprolol for 3 months). Inverted and/or bifid T waves and abnormal U wave were observed in the precordial leads. T wave alternans was observed in 4 patients in the precordial leads. These abnormal electrocardiogram features disappeared or diminished with β-blocker treatment. All spontaneous episodes of ventricular tachycardia occurred prior to sinus tachycardia and frequent polymorphic premature ventricular contractions.Bifid and/or inverted T waves, T wave alternans and abnormal U waves together with sinus tachycardia and frequent premature ventricular contractions are indicator for predicting ventricular arrhythmia and assessing the effect of β-blockers. Compliance with β-blocker treatment is a strong indicator of outcome.
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- 2013
12. [Clinical features of six patients with catecholaminergic polymorphic ventricular tachycardia]
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Dong-sheng, Zhao, Jian-hua, Shen, Jing-ping, Lu, Sheng-yong, Yu, Gang, Zhang, Jie, Geng, and Qi-jun, Shan
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Male ,Electrocardiography ,Young Adult ,Catecholamines ,Adolescent ,Tachycardia, Ventricular ,Humans ,Female ,Child - Abstract
To describe the clinical features of 6 patients with catecholaminergic polymorphic ventricular tachycardia.Clinical data including signs and symptoms, electrocardiograms, Holter monitoring electrocardiograms and echocardiography was analyzed. Definite diagnosis was made based on the mutations of RYR2 and CASQ2.From July 2002 to March 2010, 6 consecutive patients referred to our center because of syncope [4 males, mean age (13.0 ± 4.2) years] were diagnosed with CPVT by clinical evaluation and genetic testing. Their electrocardiograms showed T waves with notch or bimodal and tall U waves in right chest leads. There was no J wave, no ST-segment deviation, no prolongation or shortening of QT interval. We captured the so-called "bidirectional and(or) polymorphic ventricular tachycardia (bVT and pVT)" in 2 out of 6 patients by ECG, in 5 out of 6 patients by 24-hours Holter monitor, in 3 out of 6 patients by exercise test. All patients received β blockers and no syncope occurred during the 3 months follow-up after discharge from hospital.CPVT is an inherited cardiac channelopathy characterized by syncope and(or) sudden death relatived to motion. The ECG shows T wave alteration and tall U wave in right chest leads. The mode of its onset is bVT and(or) pVT, and can be captured by Holter easily. β blocker is a safe and effective remedy for suppressing its attack.
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- 2013
13. [Catheter ablation of focal atrial tachycardia:the topographic distribution and long-term outcome]
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Ye, Xia, Wei-zhu, Ju, Ming-long, Chen, Bing, Yang, Feng-xiang, Zhang, Hong-wu, Chen, Yu-min, Sun, Xiao-feng, Hou, Chun, Chen, Jian-gang, Zou, Qi-jun, Shan, and Ke-jiang, Cao
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Adult ,Male ,Tachycardia, Ectopic Atrial ,Young Adult ,Adolescent ,Catheter Ablation ,Humans ,Female ,Middle Aged ,Child ,Aged ,Retrospective Studies - Abstract
To explore the topographic distribution and long-term outcome of catheter ablation for focal atrial tachycardia (AT).The data of 207 patients who underwent electrophysiologic study for AT were retrospectively analyzed.A total of 200 AT were identified in 185 patients. The most common site for AT was ostium of the coronary sinus (23.8%), followed by crista terminalis (20.5%), perinodal area (20.0%), cava vena (17.8%), annulus (13.0%), and appendage (10.3%). Eighty percent AT originated from the right atrium, 17.8% originated from the left atrium. AT originated from the left atrium was more common in male than in female (25.0% vs. 13.3%, P = 0.042), while AT originated from the right atrium was more common in female than in male (69.4% vs. 86.7%, P = 0.004). Among the 185 patients, acute success ablation rate was 93.5% (n = 173). The acute success rate in the conventional mapping group was lower than that in the three-dimensional mapping group (79.3% vs. 96.5%, P0.01). During a median of 36 months follow up, the AT recurred in 20 patients (success ablation rate 88.4%). Success ablation rate was similar between the conventional mapping group and the three-dimensional mapping group (P0.05).Focal AT commonly originates from ostium of coronary sinus, crystal terminalis, perinodal area, and cava veins. There is a gender related difference in the distribution of focal AT. The radiofrequency catheter ablation yields a satisfying success rate and very low complication rate and could be the first line choice for treating ATs in experienced electrophysiological center.
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- 2012
14. [Clinical characteristics of congenital ventricular aneurysm and diverticula in inland China]
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Hong-wu, Chen, Ming-long, Chen, Bing, Yang, Wei-zhu, Ju, Feng-xiang, Zhang, Xiao-feng, Hou, Chun, Chen, Qi-jun, Shan, Jian-gang, Zou, Dong-jie, Xu, and Ke-jiang, Cao
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Adult ,Aged, 80 and over ,Heart Defects, Congenital ,Male ,China ,Adolescent ,Heart Ventricles ,Infant ,Middle Aged ,Diverticulum ,Young Adult ,Child, Preschool ,Humans ,Female ,Heart Aneurysm ,Child ,Aged - Abstract
To summarize the clinical characteristics of congenital ventricular aneurysm and diverticula in inland China.To identify the literature of congenital aneurysm and diverticula from Wanfang, China National Knowledge Infrastructure (CNKI) and PubMed databases, and to analyze the clinical characteristics of congenital aneurysm and diverticula from January of 2001 to December of 2009.A total of 116 patients [78 men, 1 - 80 (33.5 ± 21.3) years old] with congenital aneurysm or diverticula were included in 109 articles. Twenty-five patients (13 men) were congenital ventricular aneurysm, including a family of 4 patients. Ninety-one patients (65 men) were congenital ventricular diverticula. One hundred patients were detected by echocardiography during medical examination, 34 patients combined with other cardiac anomalies, 4 of which with extracardiac structures. There were 8 patients with ventricular arrhythmia, 8 patients with thrombosis, 2 patients died of cardiac rupture, 4 patients died of sudden death, surgical operation was performed in 46 patients and 3 patients received ablation procedure. All patient did not receive implantable cardioverter defibrillator (ICD) implantation.Congenital ventricular aneurysm or diverticulum is a rare cardiac malformation. Most congenital left ventricular aneurysms and diverticula are asymptomatic and detected by echocardiography. Congenital ventricular aneurysm or diverticulum may cause ventricular tachycardia, ventricular wall rupture, systemic embolization or sudden death, which had to be treated individually.
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- 2012
15. [Impact of metoprolol use in the treatment of patients with electrical-storm after cardioverter defibrillators implantation]
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Jin-bo, Yu, Bing, Yang, Dong-jie, Xu, Ming-long, Chen, Qi-jun, Shan, Jian-gang, Zou, Chun, Chen, Feng-xiang, Zhang, Xiao-feng, Hou, Wen-qi, Li, Rong, Zhang, and Ke-jiang, Cao
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Adult ,Aged, 80 and over ,Male ,Dose-Response Relationship, Drug ,Electric Countershock ,Middle Aged ,Prognosis ,Defibrillators, Implantable ,Young Adult ,Tachycardia, Ventricular ,Humans ,Female ,Anti-Arrhythmia Agents ,Aged ,Metoprolol ,Retrospective Studies - Abstract
To explore the effectiveness of the metoprolol dosage adjustment on reducing the incidence of electrical-storm (ES) in patients with Implantable Cardioverter Defibrillators (ICDs).Data from patients with ICD implantation between Jan, 2003 and Jun, 2006 in our hospital were retrospectively analyzed. ES was defined as either ≥ 3 times of ventricular tachyarrhythmias (VTAs) resulting in ICD therapy or VTAs lasting more than 30 s detected by ICD without any therapy within 24 hours.During a follow-up period of (27.5 ± 21.2) months, ES was recorded in 39 cases [34 males, average age (52.0 ± 13.1) years] out of 119 patients (32.8%) and 9 patients died after ES. During the period of storm attack, ES was successfully controlled in 25/30 patients by various interventions, including predisposing factors corrected in 5 cases, ICD reprogramming and antiarrhythmic drugs therapy optimized in 16 cases (one received intravenous injection of metoprolol), and VTAs eliminated by catheter ablation in 4 cases. ES was spontaneously resolved in the remaining 5 cases. In the chronic phase, 2 patients with Brugada syndrome were treated with Quinidine mono-therapy while the dosage of metoprolol was adjusted in the remaining 23 patients and the dosage of metoprolol was increased gradually from (26.8 ± 13.9) mg/d to (88.9 ± 53.5) mg/d without any adverse effects (9 patients received also oral amiodarone 200 mg/d). Post dosage adjustment, the total VTA episodes [(1.9 ± 1.7) times/month vs. (0.8 ± 0.6) times/month, P = 0.004], incidence of antitachycardia pacing therapies [(4.2 ± 3.8) runs/month vs. (2.3 ± 2.0) runs/month, P = 0.003], as well as electrical cardioversion or defibrillation [(1.1 ± 0.9) times/month vs. (0.4 ± 0.2) times/month, P = 0.001] were significantly decreased. ES was not controlled until a extremely high dosage [225 - 300 (255.3 ± 41.7) mg/d] of metoprolol was reached in the remaining 5 patients.Metoprolol use is essential and its dosage should be individualized in the majority of ICD recipients with ES. In approximately 1/6 patients, the dosage of metoprolol should be higher than 200 mg/d.
- Published
- 2011
16. An algorithm to predict the site of origin of focal atrial tachycardia
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Zhi-Yong, Qian, Xiao-Feng, Hou, Dong-Jie, Xu, Bing, Yang, Ming-Long, Chen, Chun, Chen, Feng-Xiang, Zhang, Qi-Jun, Shan, Ke-Jiang, Cao, and Jian-Gang, Zou
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Adult ,Male ,Tachycardia, Ectopic Atrial ,Treatment Outcome ,Body Surface Potential Mapping ,Catheter Ablation ,Humans ,Reproducibility of Results ,Female ,Diagnosis, Computer-Assisted ,Sensitivity and Specificity ,Algorithms ,Aged - Abstract
Only a few algorithms for predicting the site of origin of focal atrial tachycardia (AT) have been reported. We aimed to develop a new and more effective algorithm.Surface 12-lead electrocardiograms were collected during tachycardia and sinus rhythm in 61 patients who received successful radiofrequency ablation. P-wave polarities, durations, and amplitudes were analyzed. Predictive values of the most significant parameters were determined. An algorithm was then developed and prospectively evaluated in 30 new consecutive AT patients.Thirty-six percent (22/61) of the foci were located at the ostium of coronary sinus (CS). Other common foci included pulmonary veins (PVs, n = 15), right atrial appendage (RAA, n = 7), parahisian area (n = 7), and crista terminalis (CT, n = 3). Positive P waves in inferior leads (II, III, and aVF) and a negative P wave in lead aVR indicated high atrial origins (high CT, superior PVs, and RAA, defined as Area A), with a sensitivity of 95% and a specificity of 90%. Negative P waves in inferior leads and a positive P wave in lead aVR suggested right low septal origins (CS ostium and inferior tricuspid annulus, defined as Area B), with good sensitivity and specificity (88% and 89%, respectively). This new P-wave diagnostic algorithm correctly identified the site of origin in 90% of AT cases.Combination of data from multiple leads and regrouping of sites of origin provides a better predictive value.
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- 2010
17. Right-sided free wall accessory pathway refractory to conventional catheter ablation: lessons from 3-dimensional electroanatomic mapping
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Ke Jiang Cao, Chuan Huan Zhang, Bing Yang, Hongwu Chen, Jie Cheng, Jun Ping Sun, Fengxiang Zhang, Jun You Cui, Wei Zhu Ju, Ming Long Chen, Qi Jun Shan, Chun Chen, Yu Tao Xi, Jian Gang Zou, and Xiao Feng Hou
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Tachycardia ,Adult ,Male ,Electroanatomic mapping ,medicine.medical_specialty ,Adolescent ,medicine.medical_treatment ,Echocardiography, Three-Dimensional ,Catheter ablation ,Accessory pathway ,Atrial Function, Right ,Free wall ,Refractory ,Physiology (medical) ,Internal medicine ,medicine ,Tachycardia, Supraventricular ,Humans ,cardiovascular diseases ,business.industry ,Body Surface Potential Mapping ,Atrial activation ,Middle Aged ,cardiovascular system ,Cardiology ,Catheter Ablation ,Female ,Tricuspid Valve ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Orthodromic ,Follow-Up Studies - Abstract
Right Free Wall Accessory Pathway Ablation. Introduction: The aim of this study was to delineate the electroanatomic substrates of right-sided free wall (RFW) accessory pathways (APs) that were refractory to conventional catheter ablation utilizing 3-dimensional (3-D) mapping. Methods and Results: Eleven patients with RFW APs that failed initial conventional catheter ablation(s) by a mean of 1.9 ± 0.5 attempts were enrolled in the study. Electroanatomic mapping of the right atrium was performed during orthodromic reciprocating tachycardia in 3 patients and right ventricular pacing in 8 patients. The earliest atrial activation site, which represented the atrial insertion of the AP, was separated from the tricuspid annulus by an average of 14.3 ± 3.9 mm, and the local activation time was 27.8 ± 17.0 ms earlier than that of the corresponding annular point. One patient exhibited an AP with wide branching on the atrial side. RF ablation with an irrigated catheter successfully interrupted AP conduction in all patients without complications. Conclusions: RFW APs resistant to conventional catheter ablation might be due to unique anatomic AP features such as more epicardial course at the annulus level with atrial insertion distant from the tricuspid annulus. Electroanatomic mapping is helpful to accurately localize the atrial insertion sites of these APs and facilitates catheter ablation. (J Cardiovasc Electrophysiol, Vol. 21, pp. 1317-1324, December 2010)
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- 2010
18. [Prevalence of Epsilon wave in patients with arrhythmogenic right ventricular cardiomyopathy]
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Jing, Wang, Bing, Yang, Ming-long, Chen, Qi-jun, Shan, Jian-gang, Zou, Chun, Chen, Feng-xiang, Zhang, Xiao-feng, Hou, Hong-wu, Chen, Wei-zhu, Ju, Kai, Chen, and Ke-jiang, Cao
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Adult ,Male ,Electrocardiography ,Young Adult ,Adolescent ,Prevalence ,Humans ,Female ,Middle Aged ,Arrhythmogenic Right Ventricular Dysplasia ,Aged - Abstract
To investigate the prevalence of Epsilon wave in patients with arrhythmogenic right ventricular cardiomyopathy (ARVC).The epsilon wave was detected in 32 patients [24 men, mean age (42.3 +/- 13.3) years] with ARVC using three different electrocardiography (ECG) recording methods: standard twelve leads ECG (S-ECG), right precordial leads ECG (R-ECG) and Fontaine bipolar precordial leads ECG (F-ECG). The Epsilon wave was defined as wiggler, small spike wave and smooth potential between the end of the QRS complex and the beginning of the ST segment.Epsilon wave was detected in 37.5%, 37.5% and 50.0% patients with ARVC by S-ECG, R-ECG and F-ECG respectively. The detection rates derived from the three recording methods were similar (P0.05). The Epsilon wave was only detectable by S-ECG in one case, by R-ECG in three cases, and by F-ECG in five cases. The detection rate of Epsilon wave was 50.0% by combined use of S-ECG and R-ECG (SR-ECG), 56.3% by combined use of S-ECG and F-ECG (SF-ECG), and 65.6% by combined use of the three recording methods (SRF-ECG). The detection rate was significantly higher by SF-ECG (56.3%) and SRF-ECG (65.6%) than by S-ECG alone (37.5%, all P0.05). Most Epsilon waves detected by the S-ECG, R-ECG and F-ECG were small spiked waves.Combined use of S-ECG, F-ECG and R-ECG could increase the detection rate of Epsilon wave in patients with ARVC.
- Published
- 2009
19. [Detection of gene mutations of SCN5A in 7 patients with Brugada syndrome]
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Bin-bin, Yuan, Qi-jun, Shan, Bing, Yang, Ming-long, Chen, Jian-gang, Zou, Chun, Chen, Dong-jie, Xu, and Ke-jiang, Cao
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Adult ,Male ,DNA Mutational Analysis ,Mutation ,Humans ,Muscle Proteins ,Exons ,Middle Aged ,Sodium Channels ,Brugada Syndrome ,NAV1.5 Voltage-Gated Sodium Channel - Abstract
Brugada syndrome is linked to sodium channel mutations and could induce arrhythmias that even lead to sudden death. The purpose of this study was to detect if there was gene mutation of SCN5A in 7 patients with Brugada syndrome and explore the molecular genetic characteristics of this disease.Genomic DNA was extracted from peripheral blood of all 7 patients with Brugada syndrome and 41 pairs of PCR primers were designed to amplify all the 28 exons of SCN5A.There was no novel mutation in exons of Gene SCN5A in these patients with Brugada syndrome.Brugada syndrome might associated gene mutation or other mechanisms independent of SCN5A gene mutation.
- Published
- 2008
20. [The incessant bundle branch reentrant ventricular tachycardia in a patient with aortic valve replacement: a case report]
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Qi-jun, Shan, Ming-long, Chen, and Jian-gang, Zou
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Adult ,Heart Valve Prosthesis Implantation ,Heart Valve Prosthesis ,Tachycardia, Reciprocating ,Tachycardia, Ventricular ,Humans ,Female - Published
- 2008
21. [Tpeak-Tend interval and risk of cardiac events in patients with Brugada syndrome]
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Jin-feng, Wang, Qi-jun, Shan, Bing, Yang, Ming-long, Chen, Jian-gang, Zou, Chun, Chen, Dong-jie, Xu, and Ke-jiang, Cao
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Adult ,Male ,Electrocardiography ,Risk Factors ,Ventricular Fibrillation ,Humans ,Middle Aged ,Brugada Syndrome - Abstract
To evaluate the possible association between Tpeak-Tend (Tp-e) interval in surface standard ECG and cardiac events in patients with Brugada syndrome (BrS).Tp-e interval in surface standard ECG was compared between BrS patients (n = 23, all males) and paroxysmal supraventricular tachycardia (PSVT) patients (n = 20, all males) as well as between BrS patients with (n = 16) or without (n = 7) cardiac events.There was significant difference in Tp-e interval between BrS patients and PSVT patients [(109.57 +/- 22.86) ms vs. (88.50 +/- 13.08) ms, P0.05]. Tp-e interval was also significantly longer in BrS patients with cardiac events (syncope, clinical ventricular fibrillation and induced VF during electrophysiological study) than BrS patients without cardiac events [(118.12 +/- 20.40) ms vs. (90.00 +/- 15.27) ms, P0.05] while Tp-e interval was similar between BrS patients without cardiac events and PSVT patients (P0.05).The prolongation of Tp-e (or = 120 ms) was associated with higher cardiac events in BrS patients.
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- 2007
22. [Electrophysiological findings and ablation strategies in patients with atrial tachyarrhythmias after left atrial circumferential ablation in the treatment of atrial fibrillation]
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Ming-long, Chen, Bing, Yang, Dong-jie, Xu, Jian-gang, Zou, Qi-jun, Shan, Chun, Chen, Hong-wu, Chen, Wen-qi, Li, and Ke-jiang, Cao
- Subjects
Adult ,Male ,Pulmonary Veins ,Tachycardia ,Atrial Fibrillation ,Catheter Ablation ,Humans ,Female ,Cardiac Electrophysiology ,Heart Atria ,Middle Aged ,Aged ,Follow-Up Studies - Abstract
To report the electrophysiological findings and the ablation strategies in patients with atrial tachyarrhythmias (ATAs) or atrial fibrillation (AF) recurrence after left atrial circumferential ablation (LACA) in the treatment of AF.91 patients with AF had LACA procedure from April 2004 to May 2006, 19 of which accepted the second ablation procedure due to ATAs or AF recurrence. In all the 19 patients [17 male, 2 female, age 25 - 65 (53 +/- 12) years], 11 presented with paroxysmal AF before the first ablation procedure, 2 with persistent AF and 6 with permanent AF. Pulmonary vein potentials (PVP) were investigated in both sides in all the patients.Delayed PVP was identified inside the left circular line in 5 patients, in the right in 1 and both in 2 during sinus rhythm. "Gap" conduction was found and successfully closed guided by circular mapping catheter. In 3 cases, irregular left atrial tachycardia was caused by fibrillation rhythm inside the left ring via decremental "gap" conduction. Reisolation was done successfully again guided by 3-D mapping and made the left atrium in sinus rhythm but the fibrillation rhythm was still inside the left ring. Pulmonary vein tachycardia with 1:1 conduction to the left atrium presented in one case and reisolation stopped the tachycardia. No PVP was discovered in both sides in 4 patients but other tachycardias could be induced, including two right atrial scar related tachycardias, two supraventricular tachycardias mediated by concealed accessory pathway, one cavo-tricuspid isthmus dependent atrial flutter and one focal atrial tachycardia near the coronary sinus ostium. All the tachycardias in these 4 patients were successfully ablated with the help of routine and 3-D mapping techniques. In the rest 3, which were in AF rhythm, LACA was successfully done again. After a mean follow-up of 4 - 26 (11.5 +/- 8.5) months, 16 patients were symptom free without anti-arrhythmic drug therapy; 1 of them had frequent palpitation attack with Holter recording of atrial premature contractions; 2 of them with permanent AF became paroxysmal in one, and still in AF in the other.Reconduction between the left atrium and the pulmonary veins is the dominant factor for post-LACA ATAs and AF recurrence. Other forms of atrial tachycardias or supraventricular tachycardias may coexist with AF or sometimes trigger AF. LACA can not sufficiently modify AF substrate in some permanent AF patients.
- Published
- 2007
23. [Time distribution of ventricular arrhythmias in patients with Brugada syndrome]
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Bing, Yang, Ke-jiang, Cao, Qi-jun, Shan, Yun, Xia, Jing, Tu, Ming-long, Chen, Jian-gang, Zou, Dong-jie, Xu, Ku-lin, Li, and Chun, Chen
- Subjects
Adult ,Male ,Electrocardiography ,Risk Factors ,Ventricular Fibrillation ,Humans ,Middle Aged ,Brugada Syndrome ,Follow-Up Studies ,Time - Abstract
To study the characterization of time distribution of ventricular arrhythmias in patients with Brugada syndrome (BrS) using Holter monitoring and ICD follow-up.Patients with BrS [all male, mean age (41.07 +/- 11.49) years], were divided into ventricular fibrillation (VF) group (n = 7) and no ventricular fibrillation (N-VF) group (n = 7). Premature ventricular capture (PVC) and VF episodes were detected by Holter monitoring and ICD recording.The 24 hours total number of PVCs ranged from 0 to 74 (mean 9.61 +/- 17.23) in most of the patients and were similar between VF group and N-VF group. The percentage of PVC episodes in VF group was significantly higher than that in N-VF group from nocturnal time to early morning (22:00 to 7:00, 98.67% vs. 44.14%, P0.01). There were total 75 VF episodes during (23.18 +/- 17.96) months' follow-up in 5 patients with BrS, 93.3% of which occurred from nocturnal time to early morning (22:00 to 7:00).The episodes of PVC were enriched from nocturnal time to early morning in BrS patients, this time distribution could be a new noninvasive risk stratification factor for BrS. The episodes of VF in BrS patients were also enriched from nocturnal time to early morning and this time characteristic of episodes of VF could be used to guide drug therapy.
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- 2006
24. [Clinical efficacy of endoscopic epicardial microwave ablation for isolated atrial fibrillation]
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Biao, Yuan, Sheng, Zhao, Zhong, Zhao, Chen-jun, Huang, Hong-jun, Jiang, Qi-jun, Shan, and Ke-jiang, Cao
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Adult ,Male ,Treatment Outcome ,Atrial Fibrillation ,Catheter Ablation ,Humans ,Endoscopy ,Female ,Middle Aged ,Microwaves ,Follow-Up Studies - Abstract
To assess the efficacy of endoscopic epicardial microwave ablation, a new completely endoscopic technique, for isolated atrial fibrillation (AF).From July to October of 2004, 5 patients with isolated permanent AF underwent this procedure. We performed epicardial microwave ablation using FLEX10 probe under the guide of VasoView 5 endoscope. The probe was placed around left and right pulmonary vein orifices, the microwave application was set 65 W/90 s, and a continuous ablation line was obtained encircling the pulmonary veins. Echocardiography and electrocardiography were carried out in all patients before and after operation.Five patients received electrical conversion after ablation. Average ablation time was 35.0 min and average procedure time was 2.5 h without any procedure-related complications. At the 3-month, 6-month and 12-month follow-up, 4 patients (80%) were in sinus rhythm, associated with a reduced left atrial diameter and an improved cardiac function.Endoscopic epicardial microwave ablation for isolated atrial fibrillation appears to be safe and effective.
- Published
- 2006
25. [Left atrium linear lesion encircling pulmonary veins guided by EnSite-NavX and double-Lasso technique for paroxysmal atrial fibrillation]
- Author
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Ming-long, Chen, Jian-gang, Zou, Dong-jie, Xu, Bing, Yang, Chun, Chen, Qi-jun, Shan, Wen-qi, Li, and Ke-jiang, Cao
- Subjects
Adult ,Male ,Pulmonary Veins ,Atrial Fibrillation ,Catheter Ablation ,Humans ,Female ,Middle Aged ,Aged ,Follow-Up Studies - Abstract
To evaluate the efficacy of left atrium linear lesion encircling pulmonary veins (PV) guided by EnSite-NavX and double-Lasso technique for paroxysmal atrial fibrillation (PAF).Twenty-two patients (male 19, mean age of 48.5 years +/- 11.4 years) with symptomatic PAF were enrolled. After a geometry of the left atrium was reconstructed by EnSite-NavX system, PV ostia were marked on the map based on venography. Two Lasso catheters were placed within the ipsilateral superior and inferior PVs. Irrigated radiofrequency energy was applied at 0.5-1.0 cm of distance from the PV ostia. Continuous linear lesion was done to obtain the disappearance of pulmonary vein potentials. Patients were on propafenone and perindopril for three months after the procedure.The endpoint for ablation was reached in 21 Patients and 1 patient was not successful because of cardiac tamponade. The mean procedure time was 6.6 h +/- 1.3 h and the mean X-ray exposure time was 56.1 min +/- 18.0 min. After a mean 5.3 months +/- 2.7 months of follow-up, 10 patients were free of symptoms. Two patients had no PAF recurrence after the second procedure. Three patients had clinical recurrence of PAF in the first month. The total success rate in this study was 81% (17/21). Mortality was 0% and the overall complication rate was about 9% (2/22).Left atrium circumferential linear ablation surrounding PV ostia guided by EnSite-NavX and double-Lasso technique is effective in PAF, but some patients will need more than one procedure in order to achieve a success.
- Published
- 2006
26. [The electrophysiological study and implantable cardioverter defibrillator therapy for the patients with Brugada syndrome]
- Author
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Qi-jun, Shan, Bing, Yang, Ming-long, Chen, Jian-gang, Zou, Dong-jie, Xu, Chun, Chen, Ku-lin, Li, Pin-jun, Zhu, Xiao-bing, Wang, and Ke-jiang, Cao
- Subjects
Adult ,Electrophysiology ,Male ,Death, Sudden, Cardiac ,Ventricular Fibrillation ,Humans ,Middle Aged ,Brugada Syndrome ,Defibrillators, Implantable - Abstract
Clinical observation of electrophysiological study and implantable cardioverter defibrillator (ICD) therapy in patients with Brugada syndrome.Ten patients (all male) with Brugada wave (spontaneous or propafenone test positive in ECG) underwent electrophysiological study (EPS). The mean age was (41 +/- 10) years. They had no structural heart disease with echocardiogram and the angiogram work-up. The ICD implanted in the patients with EPS-induced ventricular fibrillation in those who were available.Three patients had the history of familial sudden cardiac death (SCD). Four patients had repeated syncope episodes, two of them had documented ventricular fibrillation during syncope episodes. The AH and HV intervals were 50 - 124 (86 +/- 21) ms and 41 - 84 (58 +/- 15) ms. The ventricular fibrillation was induced in four patients with syncope and atrioventricular reentry tachycardia in one patient with palpitation. Three patients had spontaneous or inducible atrial fibrillation. The ICD implanted in three patients with inducible ventricular fibrillation. Due to economic issue, one patient without ICD implantation had got SCD during follow-up. The patient with atrioventricular reentry tachycardia underwent a successful left atrioventricular accessory pathway ablation.The Brugada patients with syncope and high rate of inducible ventricular fibrillation in EPS are the high risk population for SCD, in whom ICD should implant promptly to prevent SCD.
- Published
- 2005
27. [Application of dynamic substrate mapping in ablation of ventricular tachycardias in arrhythmogenic right ventricular cardiomyopathy]
- Author
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Jian-Gang, Zou, Ke-Jiang, Cao, Bing, Yang, Ming-Long, Chen, Qi-Jun, Shan, Chun, Chen, and Wen-Qi, Li
- Subjects
Adult ,Male ,Catheter Ablation ,Tachycardia, Ventricular ,Humans ,Electrophysiologic Techniques, Cardiac ,Arrhythmogenic Right Ventricular Dysplasia - Abstract
To study the application of abnormal electrophysiological substrate mapping for guiding ablation of ventricular tachycardias in arrhythmogenic right ventricular cardiomyopathy (ARVC-VTs) using a non-contact mapping system.Dynamic substrate mapping was performed in three male ARVC patients during sinus rhythm. The sites of the earliest activation, exit point and activation sequence were mapped for each induced VT.Three different patterns of substrates were determined in 3 patients, which located in right ventricular outflow tract, anterior right ventricular wall, and anterolateral right ventricular wall, respectively. Five different clinical VTs [mean CL (348 +/- 65) ms] were induced. Of 5 VTs, three were originated from substrate or boundary of substrate, and two had a remote origin. One VT conducted through the substrate. Linear ablations were created between the sites of the earliest ventricular activation and the VT exit point, or across the critical isthmus. The five clinical VTs were successfully ablated. There were no VT recurrences during 20 months of follow-up.Defining the abnormal electrophysiologic VT substrates is useful for understanding the mechanisms of ARVC-VTs and determining an ablation strategy. Linear ablation across a critical isthmus or between the earliest activation and the exit point can effectively cure these arrhythmias.
- Published
- 2005
28. Antifungal Activity of Eleven Plant Essential Oils against Six Plant Pathogenic Fungi
- Author
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WU, Jian-Ting, primary, ZHANG, Yue-Li, additional, ZHANG, Bo, additional, ZHAO, Lian-Zhong, additional, LI, Chang-Song, additional, and QI, Jun-Shan, additional
- Published
- 2013
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- View/download PDF
29. Research on pathogen of peanut stem rot in Shandong Province.
- Author
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GUO Hong - can, ZHANG Yue - li, QI Jun - shan, ZHANG Bo, LI Lin, XU Zuo - ting, LI Chang - song, and ZHANG Guang - min
- Abstract
Six representative strains were selected from peanut stem rot plants collected from Shandong Province. Morphological characteristics, pathogenicity and biological characteristics of 6 representative strains were studied. Ribosomal DNA - ITS sequence analysis showed that the sequence homology of these 6 strains was up to 99% . The tested 6 isolates were confirmed as one species. The homology of DNA - ITS sequences of the 6 strains was up to more than 99% compared with Lasiodiplodia spp (synonyms Diplodia) in GenBank. According to morphological characteristics, pathogenicity test and biological characteristics of the 6 tested strains, and combined with the ribosomal rDNA - ITS sequence analysis, the pathogen of peanut stem rot from Shandong Province was identified as Diplodia gossypina [ABSTRACT FROM AUTHOR]
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- 2014
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30. Hypoxia reoxygenation induces premature senescence in neonatal SD rat cardiomyocytes.
- Author
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Feng-Xiang Zhang, Ming-Long Chen, Qi-Jun Shan, Jian-Gang Zou, Chun Chen, Bing Yang, Dong-Jie Xu, Yu Jin, and Ke-Jiang Cao
- Subjects
HEART cells ,HYPOXEMIA ,ELECTRON microscopes ,CELL proliferation ,MUSCLE cells ,RATS - Abstract
Aim: To investigate whether hypoxia reoxygenation induces premature senescence in neonatal Sprague-Dawley (SD) rat cardiomyocytes. Methods: Cardiomyocytes were isolated from neonatal SD rat heart and identified by immunohistochemistry. The control cultures were incubated at 37 °C in a humidified atmosphere of 5% CO
2 and 95% air. The hypoxic cultures were incubated in a modular incubator chamber filled with 1% O2 , 5% CO2 , and balance N2 for 6 h. The reoxygenated cultures were subjected to 1% O2 and 5% CO2 for 6 h, then 21% oxygen for 4, 8, 12, 24, and 48 h, respectively. Cell proliferation was determined using bromodeoxyuridine labeling. The ultrastructure of cardiomyocytes was observed by using an electron microscope. β-Galactosidase activity was determined by using a senescence β-galactosidase Staining Kit. p16INK4a and telomerase reverse transcriptase (TERT) mRN A levels were measured by real time quantitative PCR. TERT protein expression was determined by immunohistochemistry. Telomerase activities were assayed by using the Telo TAGGG Telomerase PCR ELISAplus kit. Results: The initial cultures consisted of pure cardiomyocytes identified by immunohistochemistry. The proportion of BrdU positive cells was reduced significantly in the hypoxia reoxygenation-treated group ( P<0.01). Under the condition of hypoxia reoxygenation, mitochondrial dehydration appeared; p16INK4a and TERT mRNA levels, β-galactosidase activity, TERT protein expression and telomerase activities were all significantly increased ( P<0.01 or P<0.05). Conclusion: These data indicate that premature senescence could be induced in neonatal SD rat cardiomyocytes exposed to hypoxia reoxygenation. Although TERT significantly increased, it could not block senescence. [ABSTRACT FROM AUTHOR]- Published
- 2007
- Full Text
- View/download PDF
31. First Report of Maize Seedling Blight Caused by Fusarium pseudograminearum in China.
- Author
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Jiang H, Ma LG, Qi K, Zhang YL, Zhang B, Ma G, and Qi JS
- Abstract
Maize ( Zea mays L.) is one of the most important food and feed crops in China, with a cultivation area of more than 40 million hectares (http://www.fao.org/faostat/en/#data/QC). In July 2021, a serious maize seeding blight occurred in Changjia Town, Gaoqing Country, Zibo City, Shandong Province, China, and the disease incidence was up to 50% in some fields. The root system of infected plants displayed poor development. The primary roots were brown and rotted. The leaves at the base of the plants were drying up, then the whole plant withered. To determine the cause agent of the disease, symptomatic roots of diseased seedlings were collected and surface-sterilized (70% ethanol for 30 s and 3% sodium hypochlorite (NaClO) for 90 s), subsequently rinsed three times with sterile distilled water, placed on potato dextrose agar (PDA), then incubated at 25°C for 2 days. Two cultures with similar morphological characteristics were purified through single-spore isolation technique and identified by morphology and molecular methods as Fusarium pseudograminearum O'Donnell & T. Aoki 1999. Plentiful macroconidia formed in 5-day-old carboxymethyl cellulose (CMC) cultures; microconidia were absent. Macroconidia were thick-walled and curved, usually 3- to 5- septa, 31.6 ± 0.6 μm × 4.8 ± 0.1 μm (n = 50). Colony pigmentation on PDA was pink to red, with white to pink aerial mycelia on PDA cultures was abundant and filled the petri dishes. For molecular identification, the rDNA internal transcribed spacer (ITS) gene and translation elongation factor 1 alpha (TEF-1α) gene of two isolates (SAIA41B and SAIA41C) were amplified with ITS1/ITS4 (White et al., 1990) and EF-1/EF-2 (O'Donnell et al., 1998), respectively. Blastn analysis of both the ITS sequence (accession numbers OM108101 and OM108102) and TEF-1α sequence (accession numbers OM142205 and OM142206) revealed 100% (481/481 bp for ITS and 637/637 bp for TEF-1α) sequence identity with the sequences of F. pseudograminearum reported in GenBank (MW699613 for ITS and JN862232 for TEF-1α). The molecular identification was further confirmed by the F. pseudograminearum species-specific PCR primers Fp1-1/Fp1-2 (Aoki and O'Donnell 1999). The expected 523-bp fragments were obtained for isolates SAIA41B and SAIA41C. In the pathogenicity test, healthy germinating maize roots (Zhengdan958) were inoculated with PDA culture blocks of isolate SAIA41C. Plants inoculated only with PDA culture blocks served as controls. Maize plants were put in petri dishes and placed in an incubator with a 12-h photoperiod at 25 oC and 100% relative humidity. Seven days later, roots of the plants inoculated with isolate SAIA41C were poorly developed and became brown necrotic and rotted, which were identical to the symptoms observed in the fields, whereas the roots of control plants were developed normally. The pathogen was re-isolated from the necrotic tissue of the inoculated roots but not from the control plants, and its identity was confirmed by PCR with the primes Fp1-1/Fp1-2 described above, fulfilling Koch's Postulates. To our knowledge, this is the first report of maize seedling blight caused by F. pseudograminearum in China. Our finding indicates the potential spread of F. pseudograminearum on maize, and more attention should be paid to prevention and control of maize seedling blight caused by F. pseudograminearum . The author(s) declare no conflict of interest. Acknowledgements: This research was supported by National Natural Science Foundation of China (No. 32102181), Shandong Provincial Natural Science Foundation (No. ZR2021QC059), Wheat Industry Technology System of Shandong Province (No. SDAIT-01-10), and Agricultural Science and Technology Innovation Project of SAAS (No. CXGC2021A38 and CXGC2021A33).
- Published
- 2022
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32. First report of Pythium aphanidermatum causing root rot of head lettuce in China.
- Author
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Qi JS, Zhang B, Ma LG, Ma G, Qin SJ, Li CS, Xu ZT, Qi K, and Zhang YL
- Abstract
Head lettuce (Lactuca sativa L.) is an important crop for fresh consumption in China. In Shandong Province, head lettuce is planted in spring and in autumn each year. Because of the on-and-off rain for three weeks, head lettuce plants planted directly into the field in Jiyang City, in July 2017, 20% of the plants rapidly showed symptoms of rotting, water-soaked lesions on roots and stem bases, and then death. The diseased plants first appeared in low-lying areas prone to water accumulation. One-millimeter pieces were excised from water-soaked roots and stem bases, dipped in a 0.2% calcium hypochlorite solution for 10 min, then placed on V8 medium, and incubated in the dark at 28°C for 5 d. Two Pythium-like strains were isolated from the roots and stems. The isolates transferred to CMA and grown for 7 d, and the morphological characteristics of the two isolates on corn meal agar (CMA) were white with dense, cottony, aerial and well-branched mycelia. The two isolates produced sporangia, oogonia, antheridia and oospores. Most of the sporangia were lobate. The oogonia were smooth, nearly globose and terminal. Oospores were globose, smooth and aplerotic. The average dimensions of 50 oogonia and oospores respectively ranged from 19.5 to 25.2 (av. 23.1) µm and 17.8 to 22.3 (av. 19.9) µm. The antheridia were broadly sac-shaped. The isolates morphological characteristics were consistent with P. aphanidermatum (van der Plaats-Niterink, 1981). The COI gene and ITS region of the rDNA were amplified and sequenced using primers FM55/FM52R (Long et al. 2012) and ITS1/ITS4 (White et al. 1990), respectively. The two aligned COI sequences were identical for both isolates, as were the two ITS sequences. BLASTn analysis of the 1,133-bp COI sequence (accession no. MT952703) resulted in a 100% identity with accession number AY129164 from Lactuca sativa, which belongs to P. aphanidermatum, and the 808-bp ITS sequence (accession no. MT921597) showed a 99% identity with Genbank accession number HQ643442 belonging to P. aphanidermatum. Koch's postulates were conducted by first soaking corn kernels for 24 h in water, and then autoclaving for 2 h at 121˚C. Isolate SDHL-1 was grown on CMA for 10 days, after which agar plugs were transferred to the sterilized corn kernels and incubated at 28℃ for approximately 15 d, until the corn kernels were covered in white hyphae. Ten healthy head lettuce plants were transplanted into a sterilized loam potting soil artificially infested with the corn inoculum (3 g inoculum per 100 g loam mixture). Inoculated plants and noninoculated controls were maintained in a greenhouse at 28°C and 100% relative humidity with a 12-h photoperiod; the experiment was repeated once. All twenty inoculated plants exhibited symptoms within one week similar to those observed. Pythium aphanidermatum was recovered only from the water-soaked roots and stem bases of inoculated plants and the re-isolated cultures again identified based on morphological characteristics and sequencing of the ITS and COI genes. No symptoms were observed on the control plants. Sclerotinia sclerotiorum is reported to cause stem base rot of L. sativa in China (Zhou et al. 2011). To our knowledge, however, this is the first report of root rot of head lettuce caused by Pythium aphanidermatum. Identification of the pathogen will assist in devising strategies to reduce yield loss.
- Published
- 2021
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33. First Report of Root and Crown Rot of American Ginseng Caused by Pythium spinosum in China.
- Author
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Qi JS, Ma LG, Zhang B, Li CS, Xu ZT, Jingyan W, Qi K, and Zhang YL
- Abstract
American ginseng (Panax quinquefolius) is a perennial herb whose dried roots are used for health care products, medicine, and food in China (Yuan et al. 2010). Shandong Province is the main area growing American ginseng and contributes more than 50% of the production in China. Wendeng city, located in the east of Shandong Peninsula, is the primary production area of American ginseng in Shandong Province since it has four distinct seasons, sufficient light, loose soil (pH 5.5~7.0), and with thus a similar geographical environment and climate conditions to the American ginseng production area of the United States and Canada. In March 2016, 2-year old American ginseng plants that were planted directly into the ground in the greenhouses in Wendeng city, contained up to 6-10% stunted plants. Water-soaked lesions were observed on the crowns and the tips of fine roots. The leaves of the infected plants became scalded, dark green starting at the top of the plants and gradually move downward. Moreover, the leaves and petioles gradually curled withered and drooped, and the whole plant collapsed. Tissue samples, 10 mm in size, were excised from the water-soaked roots and crowns of diseased plants, rinsed under running water for 24 hours, dipped in a 0.2% calcium hypochlorite solution for 10 minutes, placed on sterile filter paper to dry and then placed on V8 medium (200 mL V8 Campbell Soup, 15 g agar, 0.2 g CaCO3, and 1 L distilled water) and incubated in the dark at 28 °C for 5 days. Five Pythium-like isolates which were arachnoid-cottony on cornmeal agar were isolated and they all produced hyphal swellings, oogonia, antheridia and oospores. Oospores were globose, smooth and plerotic, with some being aplerotic. The dimensions of hyphal swellings, oogonia and oospores respectively ranged from 9.0 to 21.3 (average 14.1) µm, 12.9 to 22.5 (average 18.2) µm, and 12.5 to 20.5 (average 16.7) µm. Finger-like projections were uniformly distributed on the walls of the oogonia and the antheridia were curved rods. The five Pythium-like isolates were identified as P. spinosum based on morphological characteristics (van der Plaats-Niterink, 1981). Genomic DNA was extracted from the isolates of the Pythium sp. using a DNA extraction kit (OMEGA, U.S.A.). The cytochrome c oxidase subunit I (COI) gene and internal transcribed spacer (ITS) region rDNA were amplified and sequenced using primers FM55/FM52R (Long et al. 2012) and ITS1/ITS4, respectively (White et al.1990). The five COI sequences were aligned and were identical for all five isolates, as well as the five ITS sequences. BLASTn analysis of the 538-bp COI sequence (accession no. MT822775) resulted in a 99% identity with that of the P. spinosum strain CBS122663 (accession no. HQ708832.1), and the 916-bp ITS sequence (accession no. MN847595) showed 100% identity with Genbank accession number AB217665 belonging to P. spinosum. Koch's postulates were confirmed. Corn kernels that had been soaked in water for 24 hours in water, autoclaved for 2 hours at 121˚C and allowed to cool were inoculated with agar plugs of P. spinosum grown on corn meal agar medium (CMA) for 10 days. The inoculated corn kernels were incubated at 28 ℃ for 13~15 days, until the corn kernels were covered with white hypha of P. spinosum. Ten healthy approximately 2-years old American ginseng plants growing in Wengdeng greenhouses were transplanted into a sterilized potting soil that was artificially infested with the corn inoculum (3 g inoculum per 100 g loam mixture). Inoculated and non-inoculated control plants were maintained in a greenhouse with a roof covered with sunshade net at 28 °C and 100% relative humidity. The experiment was repeated once. Four days after inoculation (DAI), the crown of inoculated plants developed water-soaked symptoms similar to those observed in field. No symptoms developed on the control plants. By 7 DAI, the inoculated fine roots and crowns showed water-soaked lesions identical to those observed in field, whereas control plants remained symptomless. The re-isolated isolate of P. spinosum was identical morphologically and by DNA sequence analysis to the original isolate. To our knowledge, this is the first report of root rot on American ginseng caused by P. spinosum in China and worldwide. Identification of the pathogen will assist in devising strategies to protect this important medicine plant from the pathogen, and to prevent yield losses.
- Published
- 2020
- Full Text
- View/download PDF
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