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Abstract Background Targeting oncogenic histone modification by histone deacetylase inhibitors (HDACis) demonstrates promising prospects in clinical cancer treatment, whereas a notable proportion of patients cannot benefit from HDACi therapy. This study aims to explore how HDACi influences the tumor microenvironment, in order to identify potential targets for reversing the resistance to HDACi therapies. Methods Macrophage infiltration was compared between HDACi-responding and HDACi-nonresponding cancer patients. The impact of HDACis on the phagocytic capacity of macrophages was investigated through macrophage-tumor cell co-culture system. CD47 expression in tumor cell lines and patient-derived organoids was evaluated by quantitative polymerase chain reaction (QPCR) and flow cytometry. Mechanistic studies were conducted through co-immunoprecipitation (co-IP) and chromatin immunoprecipitation (ChIP). The synergistic effect of HDACis and CD47 neutralizing antibody was assessed in subcutaneous murine tumor models. Bioinformatics approaches were adopted to analyze how macrophage infiltration determines the prognostic significance of CD47 expression in cancer patients. Results High macrophage infiltration is a determinant of therapeutic non-response to HDACi, cancer patients who did not respond to HDACi exhibit massive infiltration of tumor-associated macrophages (TAMs). TAM depletion reversed the resistance to HDACi therapy. Mechanistically, HDACi impaired the phagocytic capacity of macrophages against tumor cells through epigenetically upregulating CD47 expression. Reciprocally, HDACi-upregulated CD47 polarized macrophages towards a pro-tumor M2 phenotype through SIRPα ligation. In tumor-bearing mice, HDACi monotherapy only marginally delayed tumor progression, while the concurrent neutralization of CD47 exhibited potent anti-tumor effect through re-educating TAMs towards a tumoricidal phenotype. In cancer patients, CD47 was found to determine the prognostic significance of TAMs. Conclusions Our study offers a rationale for targeting macrophage infiltration or blocking CD47 to sensitize HDACi therapies in cancer patients.

The type and content of clay minerals in tight sandstone reservoirs of the He-1 member in the Jin-30 well area of Hangjinqi in the northern margin of the Ordos Basin have a significant effect on irreducible water saturation. On the basis of core observation, the petrological characteristics, clay mineral types and occurrence forms, pore structure and irreducible water distribution of the target layer by means of X-ray diffraction analysis, high-resolution scanning electron microscopy, casting thin section analysis, one-dimensional nuclear magnetic resonance experiment and high-pressure mercury injection experiment were studied. The results show that: (1) The average content of clay minerals in the reservoir is 18.36%, and the clay minerals mainly include kaolinite, illite, chlorite and illite/smectite mixed layers. (2) There are differences in the types of clay minerals in different lithofacies: the clay minerals in lithic quartz sandstone are mainly feldspar altered kaolinite, and feldspar intragranular dissolution pores, and kaolinite intergranular pores are developed. The lithic sandstone is mainly composed of lithic and matrix altered illite, and the intragranular dissolution pores filled with illite are developed. The BRIEF REPORT is available for this paper at http://www.ykcs.ac.cn/en/article/doi/10.15898/j.ykcs.202407150157.

Climate change threatens China’s rice production, making it crucial to assess the impact of climate change and climate year type (CYT) on rice production across regions to safeguard food security. The impact of climate change under nine CYTs with different combinations of temperature and precipitation on two rice cropping systems, including single rice and double rice (early and late rice) was evaluated. The results indicate that: (1) the Northeast region was expected to undergo the greatest warming of 2.03–2.48 °C, and future climate conditions would be dominated by Warm-Humid, Warm-Normal, and Warm-Dry CYTs across all regions. (2) Climate change would significantly shorten anthesis days after sowing and maturity days after sowing of single rice by 6–12 days and 9–24 days, with little change observed for late rice (< 1 day). Late rice yield suffered more from climate change compared to single and early rice yield, declining by 8.8 %–16.13 %. (3) Different CYTs exhibited varying impacts on rice yields. Yields were projected to decrease by approximately 4.765 % to 18.645 % in Warm-Humid, Warm-Normal, and Warm-Dry CYTs. Conversely, the Northeast region was anticipated to experience an increase in yield. (4) Relationships between rice yield and meteorological factors varied by region, variety, and CYT. Among the nine CYTs, high killing degree days, mean daily temperature, mean solar radiation and warm spell duration index were the main factors influencing changes in rice yield, explaining nearly 80 % of yield change. Our results would help to develop adaptation strategies in different regions and rice cropping systems.

Abstract Natural products, with their extensive chemical diversity, distinctive biological activities, and vast reservoirs, provide a robust foundation for advancing cancer therapeutics. A comprehensive phytochemical investigation of the skins from Bufo bufo gargarizans afforded two new bufadienolide derivatives identified as bufalactamides A and B (1–2), along with six known compounds: argentinogenin (3), desacetylcinobufagin (4), desacetylcinobufaginol (5), cinobufaginol (6), bufalin (7) and gamabufalin (8). The structural elucidation of these compounds was meticulously carried out by analyses of spectroscopic data (1D and 2D NMR, HR-ESIMS), and comparison with the literature data. Plausible biosynthetic pathways for the new compounds were also discussed. Moreover, the cytotoxicity of the compounds was investigated using various cancer cell lines, including lung cancer (A549), colon cancer (HCT-116), liver cancer (SK-Hep-1), and ovarian cancer (SKOV3). Our research findings indicated that compounds 3, and 6–8 exhibit potent cytotoxic activity (IC50 100 µM). From this data, we conducted a comprehensive analysis of the structure-activity relationships among these compounds.

For improving the dynamic management performance of construction cost, the dynamic control model of cost of construction based on fuzzy neural network is studied. Consider the effect of resource allocation, construction progress and construction quality on the cost of project in construction stage, and build the cost control index for the project in stage of construction. The fuzzy logic model is used in selecting the main cost related indicators from project cost control index system at the construction stage as input neurons of the BP NN, and the BP neural network is employed to output the results of prediction of cost at the construction stage. The project cost prediction results are set as the data basis for the dynamic control of the project cost, determine the most optimistic cost, the most probable cost and the most pessimistic cost of project cost in stage of construction through key chain method, and set buffers to realize dynamic control of project cost in stages of construction. The evaluation results prove that the developed method will be able to accurately predict the project cost in stages of construction, and the cost saved in the construction phase of the project is more than 300000 yuan.

Abstract Urbanization combined with global climate change, exacerbates the urban thermal environment and hinders sustainable urban development. However, the complex relationships between land surface temperature (LST) and urban morphology are being further understood, particularly in relation to different urban development patterns, distinct topography, and 3D building morphology. Thus, this study conducted a comparative study in Chengdu and Chongqing, Southwest China. We explored the impact of comprehensive factors (including socio-economic factos, topography, land use composition, and building morphology) on LST by employing the methods of linear regression, geographical detector model, and the boosted regression trees. Our results suggest that (1) high LST was mainly observed in the central part of Chengdu but it presented multicenter aggregation trend in Chongqing; (2) Socio-economic factors were the dominant variables affecting LST in both cities; (3) land use composition and building morphology showed distinct contributions to LST among the two cities; and (4) 3D building management was more effective in Chengdu than in Chongqing. A better understanding of the impact of various influencing factors on LST will enable policy makers and planners to develop appropriate strategies for constructing climate-adaptive cities and mitigating urban heat.

Abstract TNF inhibitors have been used to treat autoimmune and autoinflammatory diseases. Here we report an unexpected mechanism underlying the therapeutic effects of TNF inhibitors in Behçet’s disease (BD), an autoimmune inflammatory disorder. Using serum metabolomics and peripheral immunocyte transcriptomics, we find that polymorphonuclear neutrophil (PMN) from patients with BD (BD-PMN) has dysregulated mevalonate pathway and subsequently increased farnesyl pyrophosphate (FPP) levels. Mechanistically, FPP induces TRPM2-calcium signaling for neutrophil extracellular trap (NET) and proinflammatory cytokine productions, leading to vascular endothelial inflammation and damage. TNF, but not IL-1β, IL-6, IL-18, or IFN-γ, upregulates TRPM2 expression on BD-PMN, while TNF inhibitors have opposite effects. Results from mice with PMN-specific FPP synthetase or TRPM2 deficiency show reduced experimental vasculitis. Meanwhile, analyses of public datasets correlate increased TRPM2 expressions with the clinical benefits of TNF inhibitors. Our results thus implicate FPP-TRPM2-TNF/NETs feedback loops for inflammation aggravation, and novel insights for TNF inhibitor therapies on BD.

Thermal barrier coatings are essential for enhancing the performance and efficiency of high-performance systems, such as aerospace engines and gas turbines, by providing thermal protection at high temperatures. This study investigates YTaO4 ceramic, a material with notable high-temperature stability, low thermal conductivity, and strong corrosion resistance, making it a promising alternative for thermal barrier coatings. However, precise measurement of YTaO4’s thermal conductivity under high-temperature conditions poses a significant challenge. To address this, we employed the 3-Omega method, a transient technique well-suited for accurately measuring thermal conductivity across a wide temperature range from 300 to 700 K. The results indicate a decreasing trend in thermal conductivity with increasing temperature, exhibiting YTaO4’s potential as a high-temperature thermal barrier coating material with superior thermal insulation properties. This study not only demonstrates the utility of the 3-Omega method for characterizing thermal properties at high temperatures but also highlights its value for advancing research in high-performance materials tailored for thermal management in advanced engineering applications.

In recent years, there has been a pronounced increase in the frequency of extreme weather events. To comprehensively examine the impact of extreme weather on ecosystem services within the Wuhan Urban Agglomeration (WUA), this study utilized meteorological station data, the Mann-Kendall (MK) test, and the Standardized Precipitation-Evapotranspiration Index (SPEI) to quantify the variation trends in heatwaves (HW) and droughts from 1961 to 2020. Then the Integrated Valuation of Ecosystem Services and Trade-offs (InVEST) model was employed to evaluate and compare the differences in water yield and climate regulation ecosystem services under various HW, droughts, and HW-drought combination scenarios. The results show that over the past 60 years, the temperature, duration, and frequency of HW have significantly increased in the WUA. Specifically, the highest HW temperature, total HW days, HW frequency, and average HW temperature showed changing trend of +0.17 ℃/decade, +1.4 day/decade, +0.19 event/decade, and +0.07 ℃/decade, respectively. The year 2000 was identified as a mutation year for HW, characterized by increased frequency and heightened severity thereafter. The SPEI value exhibited an insignificant upward trend, with 1980 marked as a mutation year, indicating a decreasing trend in drought occurrences after 1980. Heatwaves have a weakening effect on both water yield and climate regulation services, while drought significantly weakened water yield and had a relatively modest effect on climate regulation. During HW-drought composite period, the average monthly water yield showed a notable discrepancy of 60 mm compared to humid years. Besides, as heatwaves intensify, the area of low aggregation for ecosystem services expands, whereas the area of high aggregation decreases. This study provides a preliminary understanding of the impact of urban extreme weather on urban ecosystem services under changing climatic conditions.

Abstract Spatiotemporal structured light has opened up new avenues for optics and photonics. Current spatiotemporal manipulation of light mostly relies on phase-only devices such as liquid crystal spatial light modulators to generate spatiotemporal optical fields with unique photonic properties. However, simultaneous manipulation of both amplitude and phase of the complex field for the spatiotemporal light is still lacking, limiting the diversity and richness of achievable photonic properties. In this work, a simple and versatile spatiotemporal holographic method that can arbitrarily sculpt the spatiotemporal light is presented. The capabilities of this simple yet powerful method are demonstrated through the generation of fundamental and higher-order spatiotemporal Bessel wavepackets, spatiotemporal crystal-like and quasi-crystal-like structures, and spatiotemporal flat-top wavepackets. Fully customizable spatiotemporal wavepackets will find broader application in investigating the dynamics of spatiotemporal fields and interactions between ultrafast spatiotemporal pulses and matters, unveiling previously hidden light-matter interactions and unlocking breakthroughs in photonics and beyond.

Abstract As the main reservoir of coalbed gas in southeastern Sichuan, the mudstone of the Permian Longtan Formation has been drilled to obtain industrial gas, but the level of exploration and development is low. The researches on the types of lithological assemblages, reservoir characteristics, and gas-bearing properties are poor, which limits the evaluation and selection of the sweet point area for the marine-continental transitional shale gas. In this paper, by comparing the differential of different lithological distribution in the well L3, multiple discriminant functions and logging interpretation models for different lithology are established to determine the classification criteria of lithological assemblage types of shale formations. Based on the experimental results of high-temperature and high-pressure isothermal adsorption, the reservoir space distribution and gas-bearing characteristics of mudstone in different lithological assemblages are compared and analyzed. It is indicated that the four lithological assemblage types are found in the Permian Longtan Formation, including thick mudstone with the interlayer of coalbed (Type I), rich mudstone with the interlayer of sandstone and thin coalbed (Type II), sandstone interbedded with mudstone with the interlayer of coalbed (Type III), and limestone interbedded with sandstone with the interlayer of mudstone (Type IV), which are superimposed with each other. The different pore structure characteristics of mudstone in different lithological assemblages is the main influencing factor of differential gas-bearing property. The dominant lithological assemblages are Type I and Type II. Coalbed and carbonaceous mudstone are the source rock and primary storage space of adsorbed gas. Moreover, with low porosity and permeability, high breakthrough pressure and the strong sealing capacity of regional mudstone, it is easy to form the “microtrap” to store the natural gas. The sealing capacity of mudstone provides a favorable condition for gas preserve. Under the dynamic condition of hydrocarbon generation, the pressure storage box is formed, accompanied with the fine reservoir spaces and gas-bearing contents.

AlCoCrFeMo0.05Ni2 high-entropy alloy (HEA) has great potential for high-temperature applications. However, its FCC matrix will decompose during annealing, which seriously limits its high-temperature applications. Here, the lattice distortion, Gibbs free energy change, phonon spectra, density of states, and bond length distribution of the FCC matrix were calculated by first-principles calculations. The findings indicate that the primary barrier to the formation of a single homogeneous solid solution in the alloy is more closely associated with Gibbs free energy rather than the degree of lattice distortion. The vibration frequency of Al is not in sync with the overall structure, making it prone to desolvation at elevated temperatures. Furthermore, an analysis of bond lengths reveals that the Al–Ni bond length is significantly shorter than the theoretical value, facilitating the formation of a (Ni, Al)-rich phase. In contrast, the longer bond lengths of Al–Cr and Al–Mo facilitate the detachment of Cr and Mo from the (Ni, Al)-rich phase, resulting in the formation of (Cr, Mo)-rich phase. This in-depth investigation sheds light on the phase decomposition mechanism of high-entropy alloys, offering valuable insights for future research in this field.

Water resources are fundamental for sustaining natural ecosystems and human activities, playing a critical role in the sustainable development of the regional environment. Under the dual pressures of human activities and climate change, however, the stress on water resources has become increasingly evident, emerging as one of the greatest global risks for the next decade. In this study, by applying the water stress index, Lorenz curve, and Theil index, we explored the spatiotemporal patterns and inequality distribution characteristics of water resource stress across two scales: catchment and basin. Additionally, we used partial least squares regression to identify the key factors influencing water resource stress. The results indicated significant regional variations in water stress across China during 2002 to 2020. At the catchment scale, areas with a water stress index greater than 0.4 were distributed in the eastern, northeastern and northwestern regions. While at the basin scale, a north–south pattern emerged with lower stress in the south and higher stress in the north. The Haihe and Huaihe river basins exhibited the highest water stress. The Lorenz curve deviated significantly from the line of absolute equality, indicating a high degree of inequality in regional water resource stress. The Theil index increased from 1.26 to 1.50, showing a slight upward trend in inequality. Analysis of the driving factors revealed that the Yellow River Basin was primarily influenced by GDP and population, the Songhua River Basin was affected by population and urban land use, and the Southwest River Basin is driven mainly by vegetation cover. Overall, precipitation was the most critical driver affecting water stress, predominantly exerting a negative influence. This study provides a theoretical basis for alleviating regional water stress and offers valuable insights for the scientific planning and management of water resources.

Abstract Although aspirin can reduce the incidence of colorectal cancer (CRC), there is still uncertainty about its significance as a treatment for CRC, and the mechanism of aspirin in CRC is not well understood. In this study, we used aspirin to prevent AOM/DSS-induced CRC in mice, and the anti-CRC efficacy of aspirin was assessed using haematoxylin and eosin (H&E) staining and by determining the mouse survival rate and tumour size. 16S rDNA sequencing, flow cytometry (FCM), and Western blotting were also conducted to investigate the changes in the gut microbiota, tumour immune microenvironment, and apoptotic proteins, respectively. The results demonstrated that aspirin significantly exerted anti-CRC effects in mice. According to 16S rDNA sequencing, aspirin regulated the composition of the gut microbiota and dramatically reduced the abundance of Enterococcus cecorum. FCM demonstrated that there were more CD155 tumour cells and CD4 + CD25 + Treg cells showed increased TIGIT levels. Moreover, increased TIGIT expression on Treg cells is associated with reduced Treg cell functionality. Importantly, the inhibition of Treg cells is accompanied by the promotion of CD19 + GL-7 + B cells, CD8 + T cells, CD4 + CCR4 + Th2 cells, and CD4 + CCR6 + Th17 cells. Overall, aspirin prevents colorectal cancer by regulating the abundance of Enterococcus cecorum and TIGIT + Treg cells.

Based on the data of outcrop, core, logging, gas testing, and experiments, the natural gas accumulation and aluminous rock mineralization integrated research was adopted to analyze the controlling factors of aluminous rock series effective reservoirs in the Ordos Basin, NW China, as well as the configuration of coal-measure source rocks and aluminous rock series reservoirs. A natural gas accumulation model was constructed to evaluate the gas exploration potential of aluminous rock series under coal seam in the basin. The effective reservoirs of aluminous rock series in the Ordos Basin are composed of honeycomb-shaped bauxites with porous residual pisolitic and detrital structures, with the diasporite content of greater than 80% and dissolved pores as the main storage space. The bauxite reservoirs are formed under a model that planation controls the material supply, karst paleogeomorphology controls diagenesis, and land surface leaching improves reservoir quality. The hot humid climate and sea level changes in the Late Carboniferous–Early Permian dominated the development of a typical coal-aluminum-iron three-stage stratigraphic structure. The natural gas generated by the extensive hydrocarbon generation of coal-measure source rocks was accumulated in aluminous rock series under the coal seam, indicating a model of hydrocarbon accumulation under the source. During the Upper Carboniferous–Lower Permian, the relatively low-lying area on the edge of an ancient land or island in the North China landmass was developed. The gas reservoirs of aluminous rock series, which are clustered at multiple points in lenticular shape, are important new natural gas exploration fields with great potential in the Upper Paleozoic of North China Craton.

Abstract Optical spatiotemporal vortices with transverse photon orbital angular momentum (OAM) have recently become a focal point of research. In this work we theoretically and experimentally investigate optical spatiotemporal vortices with radial and azimuthal quantum numbers, known as spatiotemporal Laguerre-Gaussian (STLG) wavepackets. These 3D wavepackets exhibit phase singularities and cylinder-shaped edge dislocations, resulting in a multi-ring topology in its spatiotemporal profile. Unlike conventional ST optical vortices, STLG wavepackets with non-zero $${{{{{\boldsymbol{p}}}}}}$$ p and $${{{{{\boldsymbol{l}}}}}}$$ l values carry a composite transverse OAM consisting of two directionally opposite components. We further demonstrate mode conversion between an STLG wavepacket and an ST Hermite-Gaussian (STHG) wavepacket through the application of strong spatiotemporal astigmatism. The converted STHG wavepacket is de-coupled in intensity in space-time domain that can be utilized to implement the efficient and accurate recognition of ultrafast STLG wavepackets carried various $${{{{{\boldsymbol{p}}}}}}$$ p and $${{{{{\boldsymbol{l}}}}}}.$$ l . This study may offer new insights into high-dimensional quantum information, photonic topology, and nonlinear optics, while promising potential applications in other wave phenomena such as acoustics and electron waves.

AIM: To investigate systemic immune-inflammation index (SII), neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR) levels in patients with type 2 diabetes at different stages of diabetic retinopathy (DR). METHODS: This retrospective study included 141 patients with type 2 diabetes mellitus (DM): 45 without diabetic retinopathy (NDR), 47 with non-proliferative diabetic retinopathy (NPDR), and 49 with proliferative diabetic retinopathy (PDR). Complete blood counts were obtained, and NLR, PLR, and SII were calculated. The study analysed the ability of inflammatory markers to predict DR using receiver operating characteristic (ROC) curves. The relationships between DR stages and SII, PLR, and NLP were assessed using multivariate logistic regression. RESULTS: The average NLR, PLR, and SII were higher in the PDR group than in the NPDR group (P=0.011, 0.043, 0.009, respectively); higher in the NPDR group than in the NDR group (P

Abstract Thermoelectrics have great potential for use in waste heat recovery to improve energy utilization. Moreover, serving as a solid-state heat pump, they have found practical application in cooling electronic products. Nevertheless, the scarcity of commercial Bi2Te3 raw materials has impeded the sustainable and widespread application of thermoelectric technology. In this study, we developed a low-cost and earth-abundant PbS compound with impressive thermoelectric performance. The optimized n-type PbS material achieved a record-high room temperature ZT of 0.64 in this system. Additionally, the first thermoelectric cooling device based on n-type PbS was fabricated, which exhibits a remarkable cooling temperature difference of ~36.9 K at room temperature. Meanwhile, the power generation efficiency of a single-leg device employing our n-type PbS material reaches ~8%, showing significant potential in harvesting waste heat into valuable electrical power. This study demonstrates the feasibility of sustainable n-type PbS as a viable alternative to commercial Bi2Te3, thereby extending the application of thermoelectrics.

Classroom behavior detection is a key task in constructing intelligent educational environments. However, the existing models are still deficient in detail feature capture capability, multi-layer feature correlation, and multi-scale target adaptability, making it challenging to realize high-precision real-time detection in complex scenes. This paper proposes an improved classroom behavior detection algorithm, YOLO-AMM, to solve these problems. Firstly, we constructed the Adaptive Efficient Feature Fusion (AEFF) module to enhance the fusion of semantic information between different features and improve the model’s ability to capture detailed features. Then, we designed a Multi-dimensional Feature Flow Network (MFFN), which fuses multi-dimensional features and enhances the correlation information between features through the multi-scale feature aggregation module and contextual information diffusion mechanism. Finally, we proposed a Multi-Scale Perception and Fusion Detection Head (MSPF-Head), which significantly improves the adaptability of the head to different scale targets by introducing multi-scale feature perception, feature interaction, and fusion mechanisms. The experimental results showed that compared with the YOLOv8n model, YOLO-AMM improved the mAP0.5 and mAP0.5-0.95 by 3.1% and 4.0%, significantly improving the detection accuracy. Meanwhile, YOLO-AMM increased the detection speed (FPS) by 12.9 frames per second to 169.1 frames per second, which meets the requirement for real-time detection of classroom behavior.

IntroductionTai Chi Chuan (TCC) is a traditional Chinese mind–body exercise widely adopted in Chinese communities and colleges. However, the mindful essence of TCC is rarely emphasized during popularization. This makes it difficult for beginners to benefit from it. The present study aimed to examine the effects of a Mindfulness-enhanced Tai Chi Chuan (MTCC) intervention, which enhances mindfulness components embedded within TCC, on mental and physical health among beginners.MethodsA randomized controlled trial was conducted with 119 healthy college students new to Tai Chi Chuan training. Participants were assigned to either the MTCC group or the TCC group. Both interventions consisted of 10 weekly 90-min training sessions, with the MTCC group emphasizing and enhancing mindfulness components. Outcome measures included mindfulness, depression, anxiety, stress, and physical fitness, assessed at baseline and post-intervention.ResultsThe results showed that the MTCC group had significantly greater improvements than the TCC group in mindfulness, anxiety, stress, and health—and skill-related physical fitness. There were no significant differences between the two groups in depression.ConclusionThe findings suggest that compared to TCC, MTCC can effectively promote individuals’ physical fitness and provide additional benefits to mental well-being. MTCC can be recommended as an accessible and beneficial intervention for beginners to improve mental health and strengthen their bodies.Clinical review registrationhttps://www.chictr.org.cn/, identifier ChiCTR2200058175.

Abstract Atmospheric rivers (ARs) deliver significant and essential precipitation to the western United States (US) with consequential interannual variability. The intensity and frequency of ARs strongly influence reservoir levels, mountain snowpack, and groundwater recharge, which are key drivers of water‐resource availability and natural hazards. Between October 2022 and April 2023, western states experienced exceptionally heavy precipitation from several families of powerful ARs. Using observations of surface‐loading deformation from Global Navigation Satellite Systems, we find that terrestrial water‐storage gains exceeded 100% of normal within vital California watersheds. Independent water‐storage solutions derived from different data‐analysis and inversion methods provide an important measure of precision. The sustained storage increases, which we show are closely associated with ARs at daily‐to‐weekly timescales, alleviated both meteorological and hydrological drought conditions in the region, with a lag in hydrological‐drought improvements. Quantifying water‐storage recovery associated with extreme precipitation after drought advances understanding of an increasingly variable hydrologic cycle.

Bi(Te, Se)-based compounds have attracted lots of attention for nearly two centuries as one of the most successful commercial thermoelectric (TE) materials due to their high performance at near room temperature. Compared with 3D bulks, 2D thin films are more compatible with modern semiconductor technology and have unique advantages in the construction of micro- and nano-devices. For device applications, high average TE performance over the entire operating temperature range is critical. Herein, highly c-axis-oriented N-type Bi(Te, Se) epitaxial thin films have been successfully prepared using the pulsed laser deposition technology by adjusting the deposition temperature. The film deposited at ∼260 °C demonstrate a remarkable average power factor (PFave) of ∼24.4 μW·cm−1·K−2 over the temperature range of 305–470 K, higher than most of the state-of-the-art Bi(Te, Se)-based films. Moreover, the estimated average zT value of the film is as high as ∼0.81. We then constructed thin-film TE devices by using the above oriented Bi(Te, Se) films, and the maximum output power density of the device can reach up to ∼30.1 W/m2 under the temperature difference of 40 K. Predictably, the outstanding average TE performance of the highly oriented Bi(Te, Se) thin films will have an excellent panorama of applications in semiconductor cooling and power generation.

Abstract In contemporary large wind farms, the combination of condition-based maintenance (CBM) and time-based maintenance (TBM) has become a prevalent approach in preventive maintenance, which is an indispensable part to ensure the safe, stable and environmental operation of equipment. However, the utilization of an inappropriate maintenance strategy may result in over-maintenance or under-maintenance, leading to unstable equipment operation. Furthermore, the majority of preventive maintenance involves replacement maintenance, which may have adverse effects on the performance of wind turbines with excessive maintenance time. Therefore, this paper takes the gearbox as a case study to introduce the incomplete maintenance parameters into the failure rate function to establish a state model based on the stochastic differential equation (SDE) and describing the state change of incomplete maintenance. And then simulating the state model of the gearbox and the joint preventive maintenance strategy of TBM and CBM through examples, resulting the time-based incomplete maintenance (TBIM) is proposed based on the TBM and the incomplete maintenance, and a new joint preventive maintenance strategy incorporating TBIM and CBM is proposed. Through developing the decision-making process of the maintenance strategy to optimize the inappropriate maintenance which including over-maintenance and under-maintenance and simulating the optimized preventive maintenance strategy to compare with that of TBM and CBM and verify the superiority and effectiveness of the proposed maintenance method.

Background: Although substantial efforts have been made by researchers to develop drugs, a disappointing reality is that the emergence of drug resistance is an unavoidable reality for the majority of patients. In recent years, emerging evidence suggests a connection between drug resistance and immune dysregulation. Summary: As a ubiquitously distributed, versatile innate immune cell, macrophages play essential roles in maintaining tissue homeostasis in a steady state. Nevertheless, it is becoming aware that macrophages undermine the action of therapeutic drugs across various disease types. Reprogramming macrophage function has been proven to be effective in restoring patient responsiveness to treatment. Herein, we comprehensively reviewed how macrophages respond to drugs and the mechanisms by which they contribute to treatment unresponsiveness in cancer, inflammatory diseases, and metabolic diseases. In addition, future prospects in macrophage-based combination therapy were discussed. Key Messages: Targeting macrophages is a promising strategy for overcoming drug resistance in immune disorders.

The global repercussions of the COVID-19 pandemic on economies and public health worldwide have been profound. This study aims to examine the developmental trends of the COVID-19 pandemic, establish predictive models, and provide insights for effective control measures against potential future disease outbreaks. Considering the coexistence of both linear and nonlinear factors in COVID-19 data, conventional single-machine learning and traditional forecasting models encounter challenges in accurately predicting pandemic trends. To enhance the precision of COVID-19 pandemic predictions by integrating linear and nonlinear factors, this study proposes three combined forecasting models: CNN-LSTM-ARIMA, TCN-LSTM-ARIMA, and SSA-LSTM-ARIMA. These models leverage the strengths of deep learning in capturing nonlinear factors and the capabilities of the traditional ARIMA model in handling linear factors. Initially, LSTM and ARIMA models are used to model and predict the COVID-19 pandemic in Quebec, Canada. Subsequently, CNN models, TCN models, and the Sparrow Search Algorithm are employed to integrate predictions from the LSTM and ARIMA models. Comparative analyses of the three combined models, it was found that the CNN-LSTM-ARIMA model exhibits the highest predictive accuracy, with an MSE of 7048.26, RMSE of 83.95, MAE of 61.18, MAPE of 0.16, and $R^{2}$ of 0.95. To validate the applicability and stability of the CNN-LSTM-ARIMA model in predicting COVID-19 pandemics, Italian COVID-19 pandemic data was employed. The three combined forecasting models are established and evaluated using model evaluation metrics. The results affirm that the CNN-LSTM-ARIMA model remains the optimal choice, underscoring its high stability and suitability for COVID-19 pandemic forecasting endeavors.

Abstract The components of wind turbines are complex in structure and the working environment is harsh, which makes wind turbines face problems such as high failure rates and high maintenance costs. In this paper, the stochastic differential equation model has been established for the harsh operating environment of wind turbines, and used Brownian motion to simulate random disturbances; aiming at the problem of high failure rate of wind turbines, based on Weibull distribution, a new model has been established by combining operating time and equipment state to calculate the failure rate; in the analysis of monitoring data, the Higher-Order Moment method and Bayesian method were used to solve the parameters. The opportunity maintenance threshold curve and preventive maintenance threshold curve were obtained by analyzing Time-Based Maintenance and Condition-Based Maintenance. Therefore, the Condition-Based Opportunistic Maintenance strategy was obtained. The effectiveness of the proposed method was finally verified by arithmetic examples.

The rapid increase in population and economy, coupled with accelerated urbanization, is placing immense pressure on the water–energy–food (WEF) system. In this context, the water–energy–food nexus framework has emerged, recognizing the interdependencies and interactions among water, energy, and food systems, with the aim of optimizing resource management through cross-sectoral collaboration to promote sustainable development. Understanding the spatio-temporal differentiation patterns of the WEF nexus and elucidating the driving mechanisms behind changes in their coupling relationships is essential. This knowledge is crucial for ensuring the security of each subsystem and enhancing the overall sustainability of interconnected systems through coordinated efforts. To address these challenges, this study first established evaluation indicators for water, energy, and food security to quantify their levels and spatio-temporal dynamics. Subsequently, the degrees of coupling coordination within the WEF nexus were calculated. Finally, the WEF nexus’s spatial correlations were analyzed by using a spatial autocorrelation model. Spatial econometric models then identified key factors affecting its coordination. The results revealed significant spatial heterogeneity in water, energy, and food security across mainland China’s provinces. From 2002 to 2022, water security improved substantially in 87% of the provinces, while energy security began to improve in the eastern regions following a phase of high consumption. Food security saw significant enhancements, particularly in Inner Mongolia and the northeastern provinces. The overall coupling coordination of the WEF nexus improved across 30 provinces, progressing toward primary coordination. However, Henan and Anhui provinces experienced fluctuations in WEF nexus coordination. Spatial correlation analysis showed upward trends and increased clustering in WEF nexus coordination. Factors such as economic development and population positively influenced coordination, while economic agglomeration, education, and effective irrigation area had negative effects. This study elucidates the complex interconnections and key influencing factors within the WEF nexus, providing a reference framework and practical recommendations for equitable resource management.

Relocating poor people in mountainous areas to centralized settlement sites is an important poverty alleviation policy implemented by China and a key measure to promote stable poverty alleviation and sustainable rural development for the poor. Based on the survey data of 405 peasant households in the Panxi Area in 2021, this study constructed a structural equation model (SEM) to explore the influencing factors of peasant households’ willingness to relocate to a concentrated residence. The results showed the following: (1) Of the 405 peasant households surveyed, 20.98% were unwilling to move to centralized resettlement sites, making it more difficult to carry out the relocation policy for poverty alleviation. (2) Living environment, living conditions, important social groups, the economic benefits, living benefits, and survival benefits brought by concentrated residences, governments, and the village committees significantly influenced the willingness to relocate to a concentrated residence. In contrast, agricultural income, ecological benefits, and value benefits brought by the concentrated residence had little effect on the willingness to relocate to a concentrated residence. (3) Land force, human force, cognitive force, and national force significantly positively affected the peasant households’ willingness to relocate to a concentrated residence. This study is of great significance in promoting the implementation of poverty alleviation and relocation policy, improving the efficiency of relocation and promoting the wellbeing of peasant households.

Abstract Background Malnutrition and subsequent alterations in body composition (BC), particularly sarcopenia, are common but not yet elucidated in patients with inflammatory bowel disease (IBD); we aimed to detail the changes in BC and the characteristics of co‐occurrence of malnutrition and sarcopenia in IBD patients and to investigate its effect on quality of life. Methods This study was a multicentre, prospective, observational study involving four tertiary referral hospitals in China. The following data were collected from consecutive IBD inpatients: demographic information, medical history, recent weight change, handgrip strength (HGS) and BC parameters by bioelectrical impedance analysis (BIA). Nutritional assessments were performed through stepwise screening (Nutritional Risk Screening 2002) and diagnosis (World Health Organization‐related body mass index [BMI], subjective global assessment, European Society for Clinical Nutrition and Metabolism 2015 and Global Leadership Initiative on Malnutrition [GLIM] criteria). The quality of life was assessed by the Inflammatory Bowel Disease Questionnaire. IBD patients were compared with 1:1 sex‐, age‐ and BMI‐matched healthy controls (MHC). Results A total of 238 IBD patients (177 Crohn's disease [CD] and 61 ulcerative colitis [UC]), 68.5% male, with a mean age of 38.5 ± 14.0 years and a mean BMI of 19.8 ± 3.5 kg/m2, were recruited. Compared with MHC (n = 122), IBD patients showed significant deterioration in BC and physical function, characterized by muscle depletion (appendicular skeletal muscle mass index [ASMI], 8.0 ± 1.3 vs. 6.7 ± 1.2 kg/m2, Δ% −15.0% [−22.0%, −10.0%], P

Abstract Background Bone health and fracture risk are known to be correlated with stiffness. Both micro-finite element analysis (μFEA) and mechanical testing of additive manufactured phantoms are useful approaches for estimating mechanical properties of trabecular bone-like structures. However, it is unclear if measurements from the two approaches are consistent. The purpose of this work is to evaluate the agreement between stiffness measurements obtained from mechanical testing of additive manufactured trabecular bone phantoms and μFEA modeling. Agreement between the two methods would suggest 3D printing is a viable method for validation of μFEA modeling. Methods A set of 20 lumbar vertebrae regions of interests were segmented and the corresponding trabecular bone phantoms were produced using selective laser sintering. The phantoms were mechanically tested in uniaxial compression to derive their stiffness values. The stiffness values were also derived from in silico simulation, where linear elastic μFEA was applied to simulate the same compression and boundary conditions. Bland-Altman analysis was used to evaluate agreement between the mechanical testing and μFEA simulation values. Additionally, we evaluated the fidelity of the 3D printed phantoms as well as the repeatability of the 3D printing and mechanical testing process. Results We observed good agreement between the mechanically tested stiffness and μFEA stiffness, with R 2 of 0.84 and normalized root mean square deviation of 8.1%. We demonstrate that the overall trabecular bone structures are printed in high fidelity (Dice score of 0.97 (95% CI, [0.96,0.98]) and that mechanical testing is repeatable (coefficient of variation less than 5% for stiffness values from testing of duplicated phantoms). However, we noticed some defects in the resin microstructure of the 3D printed phantoms, which may account for the discrepancy between the stiffness values from simulation and mechanical testing. Conclusion Overall, the level of agreement achieved between the mechanical stiffness and μFEA indicates that our μFEA methods may be acceptable for assessing bone mechanics of complex trabecular structures as part of an analysis of overall bone health.

AIM:To explore the effect of intravitreal injection FasL inhibitors on corneal apoptosis, Fas, FasL expression, Treg numbers in blood and lymph nodes and rejection index in rats after corneal transplantation.METHODS:A total of 24 SD rats(24 eyes)who received penetrating keratoplasty were randomly divided into two groups: PBS group received intravitreal injection of PBS(12 rats, 12 eyes)and FasL inhibitor group(12 rats, 12 eyes). Rejection index was recorded every week and blood samples and lymph node were collected at 1, 3 and 5wk after surgery to analyze the proportions of Treg. Corneal tissue was collected for detecting the expression of Fas and FasL and number of apoptosis.RESULTS: The expression of Fas, FasL in FasL inhibitor group decreased significantly compared with the PBS group(all P

Abstract Background Guillain-Barré syndrome (GBS) is an acute inflammatory neuropathy. The Erasmus GBS Respiratory Insufficiency Score (EGRIS) and the modified Erasmus GBS Outcome Score (mEGOS) are prognostic models used in the prediction of mechanical ventilation and outcome. Thus far, there are only few biomarkers for the prognosis prediction of GBS patients, and albumin level is one that is promising. Methods Patients diagnosed with GBS from 2013 to 2022 at Renmin Hospital, Wuhan University, China, were included. Patients hospitalized between 2016 and 2022 underwent short- and long-term follow-ups. The correlations between EGRIS/mEGOS and mechanical ventilation and outcome were evaluated. Serum albumin level was examined the day after admission. Furthermore, we also investigated whether the level of serum albumin was useful in predicting disease severity or poor outcome. Results In all, 145 patients were enrolled. Nineteen patients (13.1%) who required mechanical ventilation had higher Hughes GBS disability score (HGDS) at admission and discharge (P

In American men, the incidence of prostate cancer (PC) is the highest among all types of cancer, making it the second leading cause of mortality associated with cancer. For advanced or metastatic PC, antiandrogen therapies are standard treatment options. The administration of these treatments unfortunately carries the potential risk of inducing neuroendocrine prostate cancer (NEPC). Neuroendocrine differentiation (NED) serves as a crucial indicator of prostate cancer development, encompassing various factors such as phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR), Yes-associated protein 1 (YAP1), AMP-activated protein kinase (AMPK), miRNA. The processes of autophagy and ferroptosis (an iron-dependent form of programmed cell death) play pivotal roles in the regulation of various types of cancers. Clinical trials and preclinical investigations have been conducted on many signaling pathways during the development of NEPC, with the deepening of research, autophagy and ferroptosis appear to be the potential target for regulating NEPC. Due to the dual nature of autophagy and ferroptosis in cancer, gaining a deeper understanding of the developmental programs associated with achieving autophagy and ferroptosis may enhance risk stratification and treatment efficacy for patients with NEPC.

In 2020, the European Medicines Agency approved subcutaneous (SC) vedolizumab (VDZ) for the maintenance treatment of adult patients with moderate to severe inflammatory bowel disease (IBD). This article reviews the efficacy, safety, persistence, pharmacology, patient satisfaction, and economic implications of transitioning to SC VDZ treatment and explores whether SC formulations can be recommended by the same guidelines as intravenous (IV) formulations. Clinical trials and real-world evidence indicate that transitioning from IV to SC VDZ in patients with IBD maintains clinical, biochemical, and patient-reported clinical remission and is well-tolerated, with no new safety issues identified, except for injection site reactions. Moreover, SC VDZ has an exposure–response relationship and low immunogenicity, is economical, and provides a high level of patient satisfaction. Owing to these advantages, transitioning may be advisable. In the future, more studies are needed to clarify the exact role of SC VDZ in IBD treatment, including optimization and transitioning strategies and individualized treatments based on baseline characteristics.

In quantum metrology researching, weak measurement has been widely applied in many aspects, providing weak-value amplification and noise suppressing ability. Recently, several modified schemes of weak measurement have been proposed, such as inverse, almost-balanced, and biased weak measurement. In this study, we enhance a spin-exchange relaxation-free (SERF) magnetometer using the biased weak measurement metrology technique. We establish and simulate a biased weak measurement (BWM)-enhanced SERF magnetometer model. By adjusting the pre-coupling process and analyzing the Fisher information, higher sensitivity to magnetic fields is verified in this study. 0.003 fT can be achieved through adjusting pre-coupling process and extinction ratio, which is higher than the best sensitivity of SERF magnetometer in existence. Our work paves the way for embedding weak measurement techniques into atomic magnetometer sensors and greatly improving their sensitivity.

Abstract We investigate the thermodynamical properties of color-flavor locked (CFL) quark matter at zero temperature, finite temperature, and strong magnetic field by using quasiparticle model. We find that considering CFL quark phase can significantly change the equation of state (EOS) as well as the properties of quark matter in quark stars (QSs) at finite temperature or under magnetic field within quasiparticle model. In particular, our results have shown that we can provide the large QSs within CFL quark phase from quasiparticle model by satisfying both the upper limit of $$\Lambda _{1.4}

Abstract Improving health and delaying aging is the focus of medical research. Previous studies have shown that mesenchymal stem cell (MSC) senescence is closely related to organic aging and the development of aging-related diseases such as osteoarthritis (OA). m6A is a common RNA modification that plays an important role in regulating cell biological functions, and ALKBH5 is one of the key m6A demethylases. However, the role of m6A and ALKBH5 in MSC senescence is still unclear. Here, we found that the m6A level was enhanced and ALKBH5 expression was decreased in aging MSCs induced by multiple replications, H2O2 stimulation or UV irradiation. Downregulation of ALKBH5 expression facilitated MSC senescence by enhancing the stability of CYP1B1 mRNA and inducing mitochondrial dysfunction. In addition, IGF2BP1 was identified as the m6A reader restraining the degradation of m6A-modified CYP1B1 mRNA. Furthermore, Alkbh5 knockout in MSCs aggravated spontaneous OA in mice, and overexpression of Alkbh5 improved the efficacy of MSCs in OA. Overall, this study revealed a novel mechanism of m6A in MSC senescence and identified promising targets to protect against aging and OA.

Abstract Sustained inflammatory invasion leads to joint damage and progressive disability in several autoimmune rheumatic diseases. In recent decades, targeting M1 macrophage polarization has been suggested as a promising therapeutic strategy for autoimmune arthritis. P300/CBP-associated factor (PCAF) is a histone acetyltransferase (HAT) that exhibits a strong positive relationship with the proinflammatory microenvironment. However, whether PCAF mediates M1 macrophage polarization remains poorly studied, and whether targeting PCAF can protect against autoimmune arthritis in vivo remains unclear. Commonly used drugs can cause serious side effects in patients because of their extensive and nonspecific distribution in the human body. One strategy for overcoming this challenge is to develop drug nanocarriers that target the drug to desirable regions and reduce the fraction of drug that reaches undesirable targets. In this study, we demonstrated that PCAF inhibition could effectively inhibit M1 polarization and alleviate arthritis in mice with collagen-induced arthritis (CIA) via synergistic NF-κB and H3K9Ac blockade. We further designed dextran sulfate (DS)-based nanoparticles (DSNPs) carrying garcinol (a PCAF inhibitor) to specifically target M1 macrophages in inflamed joints of the CIA mouse model via SR-A–SR-A ligand interactions. Compared to free garcinol, garcinol-loaded DSNPs selectively targeted M1 macrophages in inflamed joints and significantly improved therapeutic efficacy in vivo. In summary, our study indicates that targeted PCAF inhibition with nanoparticles might be a promising strategy for treating autoimmune arthritis via M1 macrophage polarization inhibition.

Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease, resulting in a huge medical burden worldwide. Accumulating evidence suggests that the gut microbiome and bile acids play pivotal roles during the development of NAFLD. Patients with NAFLD exhibit unique signatures of the intestinal microbiome marked by the priority of Gram-negative bacteria, decreased ratio of Firmicutes/Bacteroidetes (F/B), and increased Prevotella and Lachnospiraceae. The intestinal microbiota is involved in the metabolism of bile acids. Ursodeoxycholic acid (UDCA) is a key determinant in maintaining the dynamic communication between the host and gut microbiota. It generally shows surprising therapeutic potential in NAFLD with several mechanisms, such as improving cellular autophagy, apoptosis, and mitochondrial functions. This action is based on its direct or indirect effect, targeting the farnesoid X receptor (FXR) and various other nuclear receptors. This review aims to discuss the current studies on the involvement of the microbiome–UDCA interface in NAFLD therapy and provide prospective insights into future preventative and therapeutic approaches for NAFLD.

Abstract Most patients with inflammatory bowel disease (IBD) develop anemia, which is attributed to the dysregulation of iron metabolism. Reciprocally, impaired iron homeostasis also aggravates inflammation. How this iron‐mediated, pathogenic anemia‐inflammation crosstalk is regulated in the gut remains elusive. Herein, it is for the first time revealed that anemic IBD patients exhibit impaired production of short‐chain fatty acids (SCFAs), particularly butyrate. Butyrate supplementation restores iron metabolism in multiple anemia models. Mechanistically, butyrate upregulates ferroportin (FPN) expression in macrophages by reducing the enrichment of histone deacetylase (HDAC) at the Slc40a1 promoter, thereby facilitating iron export. By preventing iron sequestration, butyrate not only mitigates colitis‐induced anemia but also reduces TNF‐α production in macrophages. Consistently, macrophage‐conditional FPN knockout mice exhibit more severe anemia and inflammation. Finally, it is revealed that macrophage iron overload impairs the therapeutic effectiveness of anti‐TNF‐α antibodies in colitis, which can be reversed by butyrate supplementation. Hence, this study uncovers the pivotal role of butyrate in preventing the pathogenic circuit between anemia and inflammation.

Abstract Histiocytic sarcoma is an aggressive haematopoietic malignancy accounting for less than 1% of haematolymphoid neoplasms with a diagnosis based on morphology and immunophenotype of tissue biopsies with a very poor prognosis. Here, we report a 45‐year‐old man who was diagnosed with primary pulmonary histiocytic sarcoma with systemic metastases, with partial remission (PR) treated with cyclophosphamide, doxorubicin, vincristine and prednisone (CHOP) chemotherapy, but it relapsed soon after therapy above. Tests demonstrated that TMB was 21 Muts/Mb PD‐L1 expression was 90% positive, and the disease has been well‐controlled over 3 years using immune checkpoint inhibitors (nivolumab and pembrolizumab). Bioinformatic pan‐cancer analysis verified that there was the highest genetic alteration frequency of PD‐L1 in which amplification accounted for the majority of sarcoma tumour samples. Following that, we found that the genetic alteration of PD‐L1 was associated with poor prognosis in sarcoma patients in terms of overall survival (OS) (p = 1.51 × 10−4), progress‐free survival (PFS) (p = 4.90 × 10−2) and disease‐specific survival (DSS) (p = 4.90 × 10−2). To our knowledge, this may be the first reported case with high PD‐L1 expression in primary pulmonary histiocytic sarcoma who may benefit from immunotherapy such as nivolumab and pembrolizumab significantly and safely.

Summary: The development of castration-resistant prostate cancer (CRPC) is driven by intricate genetic and epigenetic mechanisms. Traf2- and Nck-interacting kinase (TNIK) has been reported as a serine/threonine kinase associated with tumor cell proliferation or unfavorable cancer behavior. The microarray approach revealed a substantial upregulation of TNIK expression levels, enabling us to investigate the functional behaviors of the TNIK gene in CRPC. Specifically, we discovered that AR suppresses TNIK gene transcription in LNCaP and C4-2 cells by forming a complex with H3K27me3. Following the reduction of AR levels induced by androgen deprivation therapy (ADT), TNIK is recruited to activate EGFR signaling through phosphorylation in C4-2 cells, thereby promoting CRPC progression. Our findings unveil a regulatory role of AR as a repressor for TNIK while also highlighting how TNIK activates the EGFR pathway via phosphorylation to drive CRPC progression. Consequently, targeting TNIK may represent an appealing therapeutic strategy for CRPC.

The vertical distribution of rocks’ radioactive heat production rate in the continental crust is the basis for exploring the deep thermal structure and explaining terrestrial heat flow distribution characteristics. In this paper, taking the Bohai Bay Basin as an example, according to the content of U, Th and K in the rocks and the lithologic composition of each structural layer of the crust, calculating the range of radioactive heat-generating elements in each structural layer. Then, systematically analyze the distribution characteristics of heat production rate in the crust. Finally, establish the vertical distribution model of heat production rate and discuss the relationship between heat flow and heat production rate. The results show that the rock heat production rate is mainly related to lithology. U and Th are primarily enriched in the upper crust with exponential distribution, while K content is unchanged. Under exponential crustal heat production rate distribution, the DU, DTh and DA in the crust are 15.195 km, 15.29 km and 21.11 km, respectively. A linear relationship exists between heat flow and heat production rate but cannot infer the thermal condition of the middle and lower crust.

Magnetic field gradients interfere with the coherence of the atomic ensemble and degrade the performance of the spin-exchange relaxation-free (SERF) atomic magnetometer. In this paper, the influence of magnetic field gradients is incorporated into the response model of single-beam atomic magnetometer, and the theoretical models of transverse relaxation rate, spin polarization and scale factor are established. Based on this model, we find that magnetic field gradients in different directions can cause varying degrees of degradation in the performance parameters of the magnetometer. The magnetic linewidths under different light power and magnetic field gradients are measured using the designed dedicated magnetic field gradients coils, and the spin polarization and magnetic field gradients relaxation rate are obtained through fitting. An unevenness and deviation of the curve are also observed in the experiments, indicating the presence of the magnetic field gradient of approximately 2 nT/cm in the magnetic shields. Furthermore, in a magnetic field gradient environment of 20 nT/cm, the mean of the long-term sensitivity decreased by 3.5 times and the standard deviation increased by 11 times. It shows that the magnetic field gradients will not only affect the signal-to-noise ratio, but also make the magnetometer more vulnerable to external interference when working. The sensitivity and stability of magnetometer will be greatly reduced. The research in this article provides a theoretical and experimental basis for eliminating the influence of magnetic field gradients and improving the accuracy of magnetic field measurements.

The present study aims to reveal wear characteristics of an impeller pump transporting the mixture of liquid and solid particles. An experimental work is conducted to detect patterns of wear of the pump components. The computational fluid dynamics (CFD) technique and the discrete element method (DEM) are jointly used to simulate the two-phase flow and wear of the pump components. After a 30-hour wear experiment, the inlet edge of the long blades exhibits wavy profiles, and the outlet part of the long blades is worn through. Regarding the volute, only the volute tongue is severely worn. At a particle diameter of 2.0 mm and a solid mass fraction of 10 %, the ratio of the average wear depth of the long blades to that of the volute reaches 64.5. Wear of the blade inlet edge depends on the normal cumulative contact energy, while the tangential cumulative contact energy contributes significantly to wear of the outlet part of the long blades. As the particle diameter increases from 1.0 mm to 3.0 mm, the average depth of wear of the long blades decreases monotonically by nearly 45 %, but the maximum depth of wear increases by about 34 %. The solid mass fraction serves as a key factor influencing wear of the pump components, especially when particles of large size are transported.

AIM: To examine the protection of ferulic acid (FA) against ionizing radiation (IR)-induced lens injury in rats, as well as the underlying mechanisms. METHODS: FA (50 mg/kg) was administered to rats for 4 consecutive days before they were given 10 Gy γ-radiation, as well as for 3 consecutive days afterward. Two weeks after radiation, the eye tissues were collected. Histological alterations were evaluated by hematoxylin-eosin staining. Enzyme linked immunosorbent assay (ELISA) was utilized to assess the activities of glutathione reductase (GR) and superoxide dismutase (SOD), as well as the levels of glutathione (GSH) and malondialdehyde (MDA) in the lenses. The protein and mRNA levels of Bcl-2, caspase-3, Bax, heme oxygenase-1 (HO-1), and glutamate-cysteine ligase catalytic subunit (GCLC) were quantified using Western blot and quantitative reverse transcription polymerase chain reaction, respectively. With nuclear extracts, the nuclear factor erythroid-2 related factor (Nrf2) protein expressions in the nuclei were also measured. RESULTS: Rats exposed to IR showed lens histological alterations which could be alleviated by FA. FA treatment reversed apoptosis-related markers in IR-induced lens, as evidenced by lower levels of Bax and caspase-3 and higher level of Bcl-2. Furthermore, IR induced oxidative damage manifested by decreased GSH level, increased MDA level, and decreased SOD and GR activities. FA boosted nuclear translocation of Nrf2 and increased the expressions of HO-1 and GCLC to inhibit oxidative stress, as evidenced by an increase in GSH, a decrease in MDA, and an increase in GR and SOD activities. CONCLUSION: FA may work well in preventing and treating IR-induced cataract through promoting the Nrf2 signal pathway to attenuate oxidative damage and cell apoptosis.

Abstract Background Chronic cerebral ischemia induces white matter injury (WMI) contributing to cognitive decline. Both astrocytes and microglia play vital roles in the demyelination and remyelination processes, but the underlying mechanism remains unclear. This study aimed to explore the influence of the chemokine CXCL5 on WMI and cognitive decline in chronic cerebral ischemia and the underlying mechanism. Methods Bilateral carotid artery stenosis (BCAS) model was constructed to mimic chronic cerebral ischemia in 7–10 weeks old male mice. Astrocytic Cxcl5 conditional knockout (cKO) mice were constructed and mice with Cxcl5 overexpressing in astrocytes were generated by stereotactic injection of adeno-associated virus (AAV). WMI was evaluated by magnetic resonance imaging (MRI), electron microscopy, histological staining and western blotting. Cognitive function was examined by a series of neurobehavioral tests. The proliferation and differentiation of oligodendrocyte progenitor cells (OPCs), phagocytosis of microglia were analyzed via immunofluorescence staining, western blotting or flow cytometry. Results CXCL5 was significantly elevated in the corpus callosum (CC) and serum in BCAS model, mainly expressed in astrocytes, and Cxcl5 cKO mice displayed improved WMI and cognitive performance. Recombinant CXCL5 (rCXCL5) had no direct effect on the proliferation and differentiation of OPCs in vitro. Astrocytic specific Cxcl5 overexpression aggravated WMI and cognitive decline induced by chronic cerebral ischemia, while microglia depletion counteracted this effect. Recombinant CXCL5 remarkably hindered microglial phagocytosis of myelin debris, which was rescued by inhibition of CXCL5 receptor C-X-C motif chemokine receptor 2 (CXCR2). Conclusion Our study revealed that astrocyte-derived CXCL5 aggravated WMI and cognitive decline by inhibiting microglial phagocytosis of myelin debris, suggesting a novel astrocyte-microglia circuit mediated by CXCL5-CXCR2 signaling in chronic cerebral ischemia.

Abstract Purpose To demonstrate the practicability of a portable instrument in assessing tear film breakup time (TFBUT): a lens attachment for smartphones (LAS). Methods By applying LAS in combination with the iPhone 12 pro, and a recordable slit-lamp microscope, we obtained TFBUT videos from 58 volunteers. The comparison between the conventional slit-lamp microscope and LAS by an experienced ophthalmologist. Moreover, we also invited an ophthalmic postgraduate student and an undergraduate student with no clinical experience to assess TFBUT in those videos. The inter-observer reliability was assessed using intraclass correlation coefficients (ICC). Results The TFBUT of 116 eyes in total was recorded. Reliability indexes were adequate. The Spearman’s correlation analysis and the intraclass correlation coefficient suggested a strong correlation between the 2 modalities (Right eye: Spearman’s r = 0.929, 95% confidence interval (CI) = 0.847–0.963, ICC = 0.978, p

Abstract Prostate cancer is a major disease that threatens men’s health. Its rapid progression, easy metastasis, and late castration resistance have brought obstacles to treatment. It is necessary to find new effective anticancer methods. Ferroptosis is a novel iron-dependent programmed cell death that plays a role in various cancers. Understanding how ferroptosis is regulated in prostate cancer will help us to use it as a new way to kill cancer cells. In this review, we summarize the regulation and role of ferroptosis in prostate cancer and the relationship with AR from the perspective of metabolism and molecular pathways. We also discuss the feasibility of ferroptosis in prostate cancer treatment and describe current limitations and prospects, providing a reference for future research and clinical application of ferroptosis.