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The purpose of this experiment was to study the effects of dietary microencapsulated compound probiotics on the growth performance, immune function, and intestinal flora of weaned piglets. Twenty-four healthy weaned piglets (Landrace x Rongchang) with similar weight were randomly divided into a control group and experimental group, with three replicates in each group and four pigs in each replicate. The control group was fed with corn-soybean meal basal diet, while the experimental group was fed with 0.1% microencapsulated compound probiotics, including Saccharomyces cerevisiae, Lactobacillus plantarum and Bacillus subtilis. The trial period was 42 days. The results showed that compared with the control group, the average daily gain, average daily feed intake, and feed-to-weight ratio of piglets in the experimental group had no significant difference, but the serum growth hormone content tended to increase. Compared with the control group, the concentrations of IgA, IgG, and IgM in the experimental group had no significant difference, but the concentrations of interleukin-1 and interleukin-6 decreased significantly (P<0.05). The relative abundance of Muribaculaceae, ruminal bacteria, Christenium and other bacteria in the experimental group increased significantly. The study indicates that adding microencapsulated compound probiotics in diet increased the content of growth hormone in piglets, reduced the content of inflammatory molecules, increased the richness and diversity of intestinal microorganisms. [ABSTRACT FROM AUTHOR]

To investigate the characteristics of rhesus monkey embryonic stem cells and to promote their clinical application, the differentiation and proliferation of rosettes neural stem cells from GFP marked rhesus monkey embryonic stem cells were studied The results showed that: 1) A stable and high-efficient neural differentiation system was established. More than 95% of the embryonic stem cells were differentiated into neural stem cells on the 12(th) days of differentiation; 2) the rosettes neural stem cells differentiated from the rhesus monkey embryonic stem cells could maintain their rosettes-shape by proliferating with bFGF/EGF; 3) the neural stem cells could differentiate into neurons after transplanted into the rhesus monkey brain. In conclusion, the rosettes neural stem cells differentiated from rhesus monkey embryonic stem cells could maintain their characteristics after proliferation with bFGF/EGF and they could survive and differentiate into neurons after transplanted into the rhesus monkey brain, which strongly supports the clinical application of neural stem cells in the future.

In the present study, the differences between addictive memory and ordinary memory were investigated by morphine-induced and food-induced conditioned place preference (CPP) in adolescent and adult mice. The result showed that: 1) morphine-induced CPP could be established in adult mice but not in adolescent mice. 2) food-induced CPP could be established both in adolescent and adult mice. This study indicated that between adolescent and adult mice, there was no difference in ordinary memory, but for addictive memory, they were quite different. Our data suggested that the development of addictive memory and ordinary memory system in mouse brain might not be paralleled.