Search

Objective: To compare fixed‐time artificial insemination (FTAI) conception rates and serum progesterone concentrations at the time of FTAI for cows treated with the original Ovsynch program (OV) with those treated with a modified Ovsynch (MO) program. Design This was a randomised clinical trial. Methods: The study used five split‐calving, pasture‐based dairy herds in Southwest Victoria, Australia. Controls (n = 851) received the OV program: day 0 gonadotropin‐releasing hormone, day 7 prostaglandin F2α (PGF), day 9 gonadotropin‐releasing hormone and FTAI at day 10. The treatment group (n = 852) received a MO program with an additional prostaglandin injection on day 8. Subsets of cows from each group were sampled for blood progesterone at the time of FTAI. Results: The treatment group demonstrated FTAI conception rates that were 7% (95% confidence interval 2%–12%) greater than the control group. After adjusting for the effect of age, days in milk at Mating Start Date and herd, the odds of conception using FTAI was 1.36 (95% confidence interval 1.12–1.66) times greater for treatment group cows compared with control group cows. The variability of serum progesterone concentrations at the time of FTAI was significantly less for treatment group cows compared with control group cows. Conclusion: For Holstein‐Friesian and Holstein‐Friesian cross‐bred cows managed in pasture‐based dairy herds in southern Australia, a MO protocol, including a second injection of prostaglandin F2α on day 8, increased FTAI conception rates compared with cows receiving the OV protocol. [ABSTRACT FROM AUTHOR]

We conducted a retrospective cohort study to validate the efficacy of the Australian multitrait fertility estimated breeding value (EBV). We did this by determining its associations with phenotypic measures of reproductive performance (i.e., submission rate, first service conception rate, and early calving). Our secondary aim was to report the associations between these reproductive outcomes and management and climate-related factors hypothesized to affect fertility. Our study population included 38 pasture-based dairy herds from the northern Victorian irrigation region in Australia. We collected records for 86,974 cows with 219,156 lactations and 438,578 mating events from the date on which managers started herd recording until December 2016, comprising both fertility-related data such as insemination records, calving dates, and pregnancy test results, and systems-related data such as production, herd size, and calving pattern. We also collected hourly data from 2004 to 2017 from the closest available weather station to account for climate-related factors (i.e., temperature humidity index; THI). Multilevel Cox proportional hazard models were used to analyze time-to-event outcomes (days to first service, days to cow calving following the planned herd calving start date), and multilevel logistic regression models for binomial outcomes (conception to first service) in the Holstein-Friesian and Jersey breeds. A 1-unit increase in daughter fertility EBV was associated with a 5.4 and 8.2% increase in the daily hazard of calving in the Holstein-Friesian and Jersey breeds respectively. These are relative increases (i.e., a Holstein-Friesian herd with a 60% 6-wk in-calf rate would see an improvement to 63.2% with a 1-unit increase in herd fertility EBV). Similar results were obtained for submission and conception rate. Associations between 120-d milk yield and reproductive outcome were complicated by interactions with 120-d protein percentage and calving age, depending on the breed and outcome. In general, we found that the reproductive performance of high milk-yielding animals deteriorated faster with age than low milk-yielding animals, and high protein percentage exacerbated the differences between low and high milk-yielding animals. Climate-related factors were also associated with fertility, with a 1-unit increase in maximum THI decreasing first service conception rate by 1.2% for Holstein-Friesians but having no statistically significant association in the Jersey breed. However, THI had a negative association in both breeds on the daily hazard of calving. Our study validates the efficacy of the daughter fertility EBV for improving herd reproductive performance and identifies significant associations between 120-d milk and protein yields and THI on the fertility of Australian dairy cows., (The Authors. Published by Elsevier Inc. and Fass Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).)

Reproductive performance in dairy cattle has declined over the last 50 years as an unintended consequence of selection for high milk yield. Since the early 2000s, dairy geneticists have released successive versions of fertility estimated breeding values (EBV) to assist in reversing this trend. At the herd level, fertility EBV can help managers accelerate improvements in reproductive performance by acting as a second selection criteria when used in tandem with a breeding index. However, use of the fertility EBV in sire selection currently varies between herd managers. The aim of this study was to better understand the reasons why herd managers choose or do not choose to select high-fertility EBV sires, using the Theory of Planned Behavior (TPB) as a social research framework. Thirty-five Victorian dairy herd managers were recruited as part of a larger study investigating the daughter fertility Australian Breeding Value and interviewed using a series of questions examining TPB constructs. The interviews were recorded and transcribed using template analysis. A wide range of herd manager types were enrolled into the study, with representation from diverse systems. Out of the 35 herd managers, 27 included fertility in their list of high-priority breeding objectives. A wide variation in results was consistent with previous studies that have demonstrated marked heterogeneity in herd manager attitudes toward bull selection. Herd manager-perceived barriers to selection of sires with high daughter fertility EBV included a lack of high daughter fertility bulls with other desirable traits, a lack of trust in the fertility EBV or in the Australian EBV system, difficulty in interpreting international proofs, information overload, semen prices, low bull reliability, and difficulty in understanding bull catalogs. Not all herd managers found the process problematic, however, particularly if a breeding consultant was employed to select all or most of the sires. Herd manager-perceived barriers for choosing to select daughter fertility as a breeding objective include a lack of awareness of the EBV, a lack of interest in genetics in general, low confidence in the impact of genetic selection for fertility, and a feeling that fertility was not important for their production system. The results of this study suggest that animal geneticists and on-farm service providers need to work together to allow the opportunities arising from appropriate use of fertility EBV to be realized more broadly across the dairy industry., (Copyright © 2021 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

Aims: The objectives of this study were to estimate the prevalence of digital dermatitis (DD) in Victoria, Australia, and to investigate which organisms are consistent with typical DD lesions. The prevalence and causative pathogens of DD are not clear yet in Australia and this paper is one of the first to explore these questions in this country., Methods: Examination and sampling of limbs was undertaken at three knackeries in Victoria, Australia. Limbs were classified as normal (N), active DD-lesion (A), dried or chronic DD-lesion (D) or suspected case of DD (S). A total of 823 cows were examined. Six skin biopsies were taken at each knackery, from which DNA was extracted for diversity profiling. Histochemical staining of samples was performed on eight of the skin biopsies., Results: DD was detected in 29.8% of all cows. The prevalence of DD was significantly higher in dairy cows (32.2%) than in beef cows (10.8%). The differential abundance of Treponema-species was significantly increased in dried lesions, compared with the normal skin biopsies. Actinobacteria, Proteobacteria, Firmicutes and Tenericutes were found to be significantly different in abundance in the DD lesions compared with normal skin biopsies. Silver staining of samples showed only mild inflammation and in two samples organisms with morphology consistent with Spirochaetes were detected., Conclusions: The calculated prevalence indicates that DD is present in Victoria, Australia. The results of diversity profiling showed that the presence of Treponema-species was significantly different between the samples of DD lesions and normal skin., (© 2019 Australian Veterinary Association.)

The objectives of this study were to assess antibody and cellular immune responses in first-lactation dairy heifers reared under a pasture-based production system and to investigate associations between immune competence and stress responsiveness, health, and productivity. A commercial vaccine was used to induce antibody and cellular immune responses and, based on measured responses, animals were classified as above average, average, and below average for each trait independently and in combination (overall immune competence). Overall immune competence phenotypic rankings were generated by combining standardized residual values for antibody and cellular responses measured in individual heifers. Cortisol responses to handling and yarding were used to assess stress responsiveness, worm egg counts were used to assess internal parasite burden, somatic cell counts to assess udder health and estimates of total daily milk volume, and milk fat and milk protein contents to assess productivity. A total of 393 Holstein-Friesian and Holstein-Friesian × Jersey crossbred heifers from 2 herds practicing seasonal calving in a pasture-fed production system were enrolled in the study. The immune competence phenotypes of individual heifers were assessed before their first mating and their subsequent performance during their first lactation was monitored. Animals with below-average antibody and cellular immune competence had higher serum cortisol concentrations compared with their counterparts following handling, suggesting they had a reduced ability to cope with management-induced stress. Furthermore, a significant negative (favorable) correlation was observed between antibody responses and stress responsiveness. Similarly, correlations between antibody responses and internal parasite burden were significant and negative (favorable). No correlations were observed between overall immune competence and milk yield, milk fat, and milk protein content., (Copyright © 2019 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

In pasture-based, seasonally calving dairy herds of southern Australia, the mating period usually consists of an initial artificial insemination period followed by a period of natural service using herd bulls. The primary objective of this study was to identify associations between individual bull- and herd-level management factors and bull fertility as measured by a pre- and postmating bull breeding soundness evaluation (BBSE). Multivariable mixed effects logistic regression models were used to identify factors associated with bulls being classified as high risk of reduced fertility at the premating and postmating BBSE. Bulls older than 4 yr of age at the premating BBSE were more likely to be classified high risk compared with bulls less than 4 yr of age. Bulls that were in herds in which concentrates were fed before mating were more likely to be classified as high risk at the postmating BBSE compared with bulls that were in herds where concentrates were not fed. Univariable analyses also identified areas in need of further research, including breed differences between dairy bulls, leg conformation and joint abnormalities, preventative hoof blocking for bulls, and mating ratios., (Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

In the pasture-based, seasonally calving dairy herds of southern Australia, the mating period usually consists of an initial artificial insemination period followed by a period of natural service using herd bulls. Bull breeding soundness evaluations (BBSE) were performed on 256 bulls from 32 dairy herds in southwest Victoria, using guidelines produced by the Australian Cattle Veterinarians, before and immediately after a single natural mating period. At the same time, herd managers were questioned regarding the management of the bulls. The objectives of this study were to describe the management practices of dairy herd bulls; to describe the causes of increased risk of reduced fertility in dairy herd bulls, as measured by a standard BBSE; and to describe the reasons for bull removal by herd managers during mating. At the premating BBSE, 19.5% of bulls were classified as high risk of reduced fertility, mostly due to physical abnormalities and reduced semen quality. At the postmating BBSE, 36.5% of bulls were classified as high risk of reduced fertility, mostly due to physical abnormalities, primarily lameness. Of the bulls used, 15.9% were removed from normal mating use by the herd manager, predominantly due to lameness and injuries. A premating BBSE is recommended in dairy herd bulls to identify bulls at risk of reduced fertility. Lameness is the most common problem in dairy herd bulls during the natural mating period, and risk factors associated with lameness in these bulls should be identified to better manage herd bulls., (Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

One of the major challenges for dairy producers is to produce, harvest, and store high-quality colostrum and feed it to their replacement heifer calves. Limited published data are available in Australia regarding the relationship between colostrum management, hygiene, and quality. The objectives of this study were to investigate (1) the colostrum storage and handling practices carried out on farm; (2) the immunoglobulin concentration and bacterial composition of colostrum being fed to replacement dairy heifer calves; (3) the percentage of colostrum being fed to replacement dairy heifer calves that meet industry recommendations; and (4) risk factors for bacterial contamination of colostrum. The study was carried out on 24 dairy farms located near Rochester, Victoria, Australia. Two hundred forty colostrum samples were collected (10 samples per farm). Each farm harvested and stored first-milking colostrum under normal farm conditions. A 10-mL sample of the colostrum was collected in a sterile container immediately before feeding, and a Brix refractometer reading was taken. The samples were then frozen at -4°C and submitted for bacterial concentration analysis. Fifty-eight percent of colostrum samples met the recommended industry standard of a total plate count (TPC) of <100,000cfu/mL, and 94% of colostrum samples met the recommended industry standard of total coliform count (TCC) of 10,000cfu/mL. However, when all the current industry recommendations for TPC, TCC, and Brix refractometer percentage for colostrum quality were considered, only 23% of the samples met all standards. These findings demonstrate that a large number of calves were at risk of receiving colostrum of poor quality, with high bacterial loads that may have interfered with the acquisition of transfer of passive immunity and affected calf health. Further investigation is required to identify the farm-specific factors that may influence the level of bacterial contamination of colostrum. Recommendations as a result of this study include refrigeration of excess colostrum shortly (within 1h) after collection and thorough disinfection of the calf feeding apparatus before use., (Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

Common health problems observed during peripartum include milk fever, mastitis, fatty liver disease, ketosis, dystocia, retained placenta, metritis, hypomagnesaemia and abomasal displacements. The increased incidence of health problems observed during the periparturient period can be partly attributed to suboptimal immune responses. Factors contributing to decreased periparturient immunity include the act of parturition itself, impaired leukocytic activity, effects of colostrogenesis and lactogenesis, and associated hypocalcemia and negative energy balance. Nutritional and other management strategies represent a relevant short-term strategy aimed at improving the health and welfare of the transitioning cow. Additionally, it is important to consider improving the health of dairy herds through the genetic selection of animals with enhanced robustness by identifying those with superior disease resistance or resilience in the face of infection. As a consequence these animals are better able to cope with the production and environmental stresses. These may provide long-term selection strategies for improving the health and welfare of the transitioning cow particularly when combined with sound management practices, allowing dairy cattle to reach their full genetic potential., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

The objective of this study was to determine the repeatability of ranking Holstein-Friesian heifers reared in an Australian pasture-based production system for immune responses (IR) when ranking was based on secondary versus tertiary IR. Further objectives were to investigate associations between IR and stress responsiveness, ADG and resistance to internal parasites. A total of 100 heifers were IR phenotyped at 5 to 6 mo of age and again at 12 to 13 mo of age using commercial vaccine antigens to induce measurable IR. Antibody production to tetanus toxoid (TT) was used to assess antibody-mediated IR (AMIR), and delayed-type hypersensitivity reactions to vaccine antigens were used to assess cell-mediated IR (CMIR). Changes in serum cortisol and haptoglobin were used to assess stress responsiveness and fecal egg counts used as a measure of resistance to internal parasites. Based on testing, animals were categorized as either average to above-average (High) or low responders for IR. Secondary and tertiary AMIR were well correlated (r = 0.651, adjusted R(2) = 0.418, P < 0.0001), whereas correlations between secondary and tertiary CMIR were poor (r = 0.078, R(2) = –0.004, P = 0.450). A Cohen kappa (κ) test of agreement was used to test the consistency of ranking of individual animal for IR and, therefore, the ability to consistently identify low immune responder animals within the herd across test periods. The consistency of ranking (High versus low) was moderately high for AMIR (κ = 0.445), poor for CMIR (κ = –0.055), and fair to moderate for combined IR (κ = 0.395). High AMIR phenotype animals had significantly higher serum cortisol concentrations than their low immune responder counterparts (P = 0.045). A similar relationship was observed in heifers categorized for CMIR, with High CMIR responders having higher serum cortisol concentrations than their low responder counterparts (P = 0.008). High AMIR calves had a higher ADG compared with low AMIR calves (0.72 ± 0.02 versus 0.66 ± 0.06 kg/d; P = 0.009). Serum haptoglobin concentrations and worm egg counts were very low and could not be used to investigate associations with immune responsiveness. It is concluded that secondary and tertiary antibody responses to TT were well correlated in the Holstein-Friesian heifers in this study and that by using the testing procedure described here, low antibody responders were able to be consistently identified in the herd.

Ruminal pH and serum concentrations of haptoglobin (Hp) were measured in order to assess the risk of subacute ruminal acidosis (SARA) in grazing cows offered rolled wheat grain twice daily in the dairy at milking (Control group; n= 64), or as a partial mixed ration (PMR group; n= 64) on a feedpad. Cows were allocated various levels of the supplement (8, 10, 12 or 14 kg dry matter/day). Ruminal pH was measured in 16 rumen-fistulated cows (eight PMR and eight Control group cows), using indwelling pH meters, recording every 10 min for 14 days. Serum Hp was analysed in samples collected from 125 cows. No differences in ruminal pH or serum Hp concentration were found between treatment groups, or levels of feeding. It was concluded that, using ruminal pH patterns and Hp as markers of SARA at the feeding levels used in this study, there were no differences between grazing cows fed the supplement either as grain in the dairy or as a PMR fed on a feedpad., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

The effects of feeding and management systems on the health and welfare of grazing dairy cows were investigated by comparing the claw health of cows fed grain during milking and pasture silage in the paddock (Control), with cows fed a grain-based partial mixed ration (PMR) on a concrete feed pad. Cows were assessed on three occasions during lactation: (1) early lactation (20-81 days in milk [DIM]) before allocation to feeding treatments; (2) mid-lactation (97-158 DIM) immediately following an intensive feeding experiment, and (3) late lactation (173-243 DIM) several months after return to initial management groups. At the final examination, claw puncture resistance was measured. The results showed that for the most prevalent lesions (white line disease, paintbrush haemorrhage and traumatic bruising), there was no effect of feeding system or amount of supplement on the presence of the moderate to severe forms in early lactation, but cows were more likely to have a particular lesion at the second assessment if it was present in early lactation. Puncture resistance of the claw was not related to presence of a lesion for any of the most prevalent lesion types. It was concluded for this herd that for most indicators of claw health, there was no overall effect of different feeding systems (supplement fed during milking or on a feed pad) or amount of supplement., (Copyright © 2013. Published by Elsevier Ltd.)

Automated walk-over weighing systems can be used to monitor liveweights of cattle. Minimal literature exists to describe agreement between automated and static scales, and no known studies describe repeatability when used for daily measurements of dairy cows. This study establishes the repeatability of an automated walk-over cattle-weighing system, and agreement with static electronic scales, when used in a commercial dairy herd to weigh lactating cows. Forty-six lactating dairy cows from a seasonal calving, pasture-based dairy herd in southwest Victoria, Australia, were weighed once using a set of static scales and repeatedly using an automated walk-over weighing system at the exit of a rotary dairy. Substantial agreement was observed between the automated and static scales when assessed using Lin's concordance correlation coefficient. Weights measured by the automated walkover scales were within 5% of those measured by the static scales in 96% of weighings. Bland and Altman's 95% limits of agreement were -23.3 to 43.6 kg, a range of 66.9 kg. The 95% repeatability coefficient for automated weighings was 46.3 kg. Removal of a single outlier from the data set increased Lin's concordance coefficient, narrowed Bland and Altman's 95% limits of agreement to a range of 32.5 kg, and reduced the 95% repeatability coefficient to 18.7 kg. Cow misbehavior during walk-over weighing accounted for many of the larger weight discrepancies. The automated walk-over weighing system showed substantial agreement with the static scales when assessed using Lin's concordance correlation coefficient. This contrasted with limited agreement when assessed using Bland and Altman's method, largely due to poor repeatability. This suggests the automated weighing system is inadequate for detecting small liveweight differences in individual cows based on comparisons of single weights. Misbehaviors and other factors can result in the recording of spurious values on walk-over scales. Excluding outlier weights and comparing means of 7 consecutive daily weights may improve agreement sufficiently to allow meaningful assessment of small short-term changes in automated weights in individuals and groups of cows., (Copyright © 2013 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

The aim of this study was to compare the reproductive performance, milk production, live weight, and body condition loss during early lactation of purebred Holstein (H) cows to Jersey x H (J x H) crossbred cows in 4 Victorian herds. Cows of H and J x H breeding were managed together within each herd, and all herds had a seasonally concentrated calving pattern that commenced in early spring (July). All crossbred cows included in the study were 25, 50, or 75% H and were considered collectively as J x H regardless of the sire and dam breeds used to reach those percentages. Each herd owner provided records of reproductive performance and milk production. Compared with H cows, J x H cows had higher first-service conception rates (52 vs. 42%), higher percentages confirmed pregnant by 6 (68 vs. 54%) and 14 wk (86 vs. 78%) after the first day of inseminating, and lower final not-in-calf rates (11 vs. 16%); however, these differences were not observed in all herds. A random selection of H and J x H cows had body condition assessed on 3 occasions between the start of calving and the first day of the artificial insemination program. The selected cows were also weighed on the final occasion. Overall, body condition scores were slightly higher for J x H cows than for H cows, but changes in body condition score between calving and the start of inseminating were similar between breed groups. The H cows were 40 kg heavier than J x H cows and had daily milk yields in early lactation that were 2.2 kg higher. Daily yields of milk fat and protein did not differ between H and J x H cows during the study period. The improved reproductive performance of J x H cows compared with H cows may render them more suitable for use in dairy herds with seasonally concentrated calving patterns. Their improved reproductive performance was not associated with differences in condition loss in early lactation.

Objective: To compare the effectiveness of three treatments for sole injuries in dairy cows and to evaluate the ease and speed of applying the treatments., Design: A randomised clinical trial., Procedure: The three forms of treatment were elevating the affected claw of a lame cow with a wooden block glued to the unaffected claw (39 cows), elevating the affected claw with a rubberised shoe glued to the unaffected claw (42 cows) or applying a padded bandage to the whole foot of a lame cow (31 cows)., Results: The percentages of cows that recovered from lameness at 3 and 7 days after treatment were significantly higher for cows treated with wooden blocks (P = 0.013 and P = 0.008) or rubberised shoes (P = 0.026 and P = 0.0003) than padded bandages. Cows treated with blocks had a recovery rate of 48.7% at day three and 65.8% at day seven, and cows treated with rubberised shoes, rates of 45.2% and 76.2%, respectively. Cows which had a bandage applied to the foot had recovery rates of 19.4% at day three and 32.3% at day seven. However, by day 14, there was no significant difference in recovery rate between the three treatments. Rubberised shoes were shown to offer a number of advantages over wooden blocks. The shoes were significantly quicker to attach than blocks (median of 9.5 minutes compared with 14.0 minutes, P < 0.0001) and remained on the claw longer (57.1% of rubberised shoes remained at 30 days compared to 30.8% of blocks, P = 0.025). The rubberised shoes also offered an advantage over wooden blocks for operator safety and health by reducing contact between the potentially hazardous adhesive used to attach both devices and the skin of the person applying the device., Conclusions: Rubberised shoes are a superior method of elevating an affected claw in conditions of lameness likely to respond to removal of weightbearing forces.

Cases of anthelmintic resistance on 3 goat farms in Gippsland were investigated. On the first farm Haemonchus contortus, Trichostrongylus colubriformis and Ostertagia (Teladorsagia) circumcincta were found to be resistant to fenbendazole, but the first 2 species were fully susceptible to levamisole. On the second farm a population of T. colubriformis, resistant to concurrent full doses of levamisole and a benzimidazole, was found to retain this resistance when transferred to sheep. On the third farm, heavy mortality due to Ostertagia and Trichostrongylus spp burdens was arrested only by the use of concurrent full doses of levamisole and oxfendazole; no single anthelmintic was found to be effective. A disturbing rise in the incidence of clinical helminthiosis, often accompanied by anthelmintic resistance, occurred on goat farms in Gippsland early in 1984.