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31 results on '"Puszko, Anna"'

1. Insights from structure-activity relationships and the binding mode of peptidic α-ketoamide inhibitors of the malaria drug target subtilisin-like SUB1

Author

Legru, Alice, Batista, Fernando A., Puszko, Anna K., Bouillon, Anthony, Maurel, Manon, Martinez, Mariano, Ejjoummany, Abdelaziz, Ortega Varga, Laura, Adler, Pauline, Méchaly, Ariel, Hadjadj, Margot, Sosnowski, Piotr, Hopfgartner, Gérard, Alzari, Pedro M., Blondel, Arnaud, Haouz, Ahmed, Barale, Jean-Christophe, and Hernandez, Jean-François

Published
2024
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6. Stimuli-Responsive Oligourea Molecular Films

Author

Grempka, Arkadiusz, Dziubak, Damian, Puszko, Anna K., Bachurska-Szpala, Paulina, Ivanov, Maxim, Vilarinho, Paula M., Pulka-Ziach, Karolina, and Sek, Slawomir

Abstract

We have designed and synthesized a helical cysteamine-terminated oligourea foldamer composed of ten urea residues featuring side carboxyl and amine groups. The carboxyl group is located in proximity to the C-terminus of the oligourea and hence at the negative pole of the helix dipole. The amine group is located close to the N-terminus and hence at the positive pole of the helix dipole. Beyond the already remarkable dipole moment inherent in oligourea 2.5 helices, the incorporation of additional charges originating from the carboxylic and amine groups is supposed to impact the overall charge distribution along the molecule. These molecules were self-assembled into monolayers on a gold substrate, allowing us to investigate the influence of an electric field on these polar helices. By applying surface-enhanced infrared reflection–absorption spectroscopy, we proved that molecules within the monolayers tend to reorient themselves more vertically when a negative bias is applied to the surface. It was also found that surface-confined oligourea molecules affected by the external electric field tend to rearrange the electron density at urea groups, leading to the stabilization of the resonance structure with charge transfer character. The presence of the external electric field also affected the nanomechanical properties of the oligourea films, suggesting that molecules also tend to reorient in the ambient environment without an electrolyte solution. Under the same conditions, the helical oligourea displayed a robust piezoresponse, particularly noteworthy given the slender thickness of the monolayer, which measured approximately 1.2 nm. This observation demonstrates that thin molecular films composed of oligoureas may exhibit stimulus–responsive properties. This, in turn, may be used in nanotechnology systems as actuators or functional films, enabling precise control of their thickness in the range of even fractions of nanometers.

Published
2024
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10. Additional file 3 of Neuropilin 1 and Neuropilin 2 gene invalidation or pharmacological inhibition reveals their relevance for the treatment of metastatic renal cell carcinoma

Author

Dumond, Aurore, Brachet, Etienne, Durivault, Jérôme, Vial, Valérie, Puszko, Anna K., Lepelletier, Yves, Montemagno, Christopher, Pagnuzzi-Boncompagni, Marina, Hermine, Olivier, Garbay, Christiane, Lagarde, Nathalie, Montes, Matthieu, Demange, Luc, Grépin, Renaud, and Pagès, Gilles

Abstract

Additional file 3: Fig. S2. Effects of NRP1 or NRP2 gene invalidation in RENCA cells. (A) NRP1 and NRP2 protein levels were evaluated by flow cytometry in control (RENCA), in #NRP1 4.1.7 and #NRP2 5.1.8 clones. (B) Effects of NRPs KO on RENCA cell metabolic activity measured by MTT assays. (C) Effects of NRPs KO in RENCA cells on the VEGFA and VEGFC protein levels measured by ELISA. *p

Published
2021
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11. Additional file 6 of Neuropilin 1 and Neuropilin 2 gene invalidation or pharmacological inhibition reveals their relevance for the treatment of metastatic renal cell carcinoma

Author

Dumond, Aurore, Brachet, Etienne, Durivault, Jérôme, Vial, Valérie, Puszko, Anna K., Lepelletier, Yves, Montemagno, Christopher, Pagnuzzi-Boncompagni, Marina, Hermine, Olivier, Garbay, Christiane, Lagarde, Nathalie, Montes, Matthieu, Demange, Luc, Grépin, Renaud, and Pagès, Gilles

Abstract

Additional file 6: Fig. S5. In-vivo effects of NRPa-308 on mice weight. The weight of nude mice xenografted with 786-O cells and treated with increasing doses of NRPa-308 was evaluated once a week.

Published
2021
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12. Additional file 2 of Neuropilin 1 and Neuropilin 2 gene invalidation or pharmacological inhibition reveals their relevance for the treatment of metastatic renal cell carcinoma

Author

Dumond, Aurore, Brachet, Etienne, Durivault, Jérôme, Vial, Valérie, Puszko, Anna K., Lepelletier, Yves, Montemagno, Christopher, Pagnuzzi-Boncompagni, Marina, Hermine, Olivier, Garbay, Christiane, Lagarde, Nathalie, Montes, Matthieu, Demange, Luc, Grépin, Renaud, and Pagès, Gilles

Abstract

Additional file 2: Fig. S1. Study of down-regulation of NRPs by shRNA in 786-O cells. (A-B) Effects of the downregulation of NRP by shRNA on NRP1 and NRP2 mRNA expression measured by qPCR. (C) Effects on cell metabolic activity measured by MTT assays. (D) Down-regulation of NRPs decreased cell migration. Bevacizumab increased this effect for NRP1 down-regulation. (E) Down-regulation of NRPs had no effect on VEGFA and VEGFC production measured by ELISA. *p

Published
2021
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13. Additional file 1 of Neuropilin 1 and Neuropilin 2 gene invalidation or pharmacological inhibition reveals their relevance for the treatment of metastatic renal cell carcinoma

Author

Dumond, Aurore, Brachet, Etienne, Durivault, Jérôme, Vial, Valérie, Puszko, Anna K., Lepelletier, Yves, Montemagno, Christopher, Pagnuzzi-Boncompagni, Marina, Hermine, Olivier, Garbay, Christiane, Lagarde, Nathalie, Montes, Matthieu, Demange, Luc, Grépin, Renaud, and Pagès, Gilles

Abstract

Additional file 1: Table S1. List of oligonucleotides used in qPCR experiments. Table S2. Recapitulative table of the expression of NRP1 and NRP2 in KO and knock-down cells. Table S3. Recapitulative table of the expression of VEGFA and VEGFC in KO and knock-down cells. Table S4. NRP1 and NRP2 binding site descriptors computed with DogSite Scorer (Nb: number, HBA: Hydrogen Bond Acceptor, HBD: Hydrogen Bond Donor, AA: amino acids).

Published
2021
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14. Additional file 5 of Neuropilin 1 and Neuropilin 2 gene invalidation or pharmacological inhibition reveals their relevance for the treatment of metastatic renal cell carcinoma

Author

Dumond, Aurore, Brachet, Etienne, Durivault, Jérôme, Vial, Valérie, Puszko, Anna K., Lepelletier, Yves, Montemagno, Christopher, Pagnuzzi-Boncompagni, Marina, Hermine, Olivier, Garbay, Christiane, Lagarde, Nathalie, Montes, Matthieu, Demange, Luc, Grépin, Renaud, and Pagès, Gilles

Abstract

Additional file 5: Fig. S4. NRPs KO in RENCA tumor cells inhibited experimental RCC growth in immunodeficient mice. (A) Experimental tumors in nude mice were obtained after injection of 3 × 105 control (Ctrl, 10 mice) or NRPs KO RENCA cells (5 mice for each condition). One NRP1 KO clone (4.1 7) and one NRP2 KO clone (5.1 8) were injected. Tumor volume at the indicated times is presented. Each curve stands for an individual mouse.

Published
2021
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16. Neuropilin 1 and Neuropilin 2 Gene Invalidation or Pharmacological Inhibition Reveals Their Relevance for the Treatment of Metastatic Renal Cell Carcinoma

Author

Dumond, Aurore, primary, Brachet, Etienne, additional, Durivault, Jérôme, additional, Vial, Valérie, additional, Puszko, Anna, additional, Lepelletier, Yves, additional, Montemagno, Christopher, additional, Pagnuzzi-Boncompagni, Marina, additional, Hermine, Olivier, additional, Garbay, Christiane, additional, Lagarde, Nathalie, additional, Montes, Matthieu, additional, Demange, Luc, additional, Grépin, Renaud, additional, and Pagès, Gilles, additional

Published
2020
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25. The effect of wool hydrolysates on squamous cell carcinoma cells in vitro. Possible implications for cancer treatment

Author

Damps, Tatsiana, primary, Laskowska, Anna Katarzyna, additional, Kowalkowski, Tomasz, additional, Prokopowicz, Monika, additional, Puszko, Anna Katarzyna, additional, Sosnowski, Piotr, additional, Czuwara, Joanna, additional, Konop, Marek, additional, Różycki, Krzysztof, additional, Borkowska, Joanna Karolina, additional, Misicka, Aleksandra, additional, and Rudnicka, Lidia, additional

Published
2017
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30. Urea-Peptide Hybrids as VEGF-A 165 /NRP-1 Complex Inhibitors with Improved Receptor Affinity and Biological Properties.

Author

Puszko, Anna K., Sosnowski, Piotr, Rignault-Bricard, Rachel, Hermine, Olivier, Hopfgartner, Gérard, Pułka-Ziach, Karolina, Lepelletier, Yves, and Misicka, Aleksandra

Subjects
*VASCULAR endothelial growth factors, *LIGAND binding (Biochemistry), *PEPTIDE bonds, *CELL membranes
Abstract

Neuropilin-1 (NRP-1), the major co-receptor of vascular endothelial growth factor receptor-2 (VEGFR-2), may also independently act with VEGF-A165 to stimulate tumour growth and metastasis. Therefore, there is great interest in compounds that can block VEGF-A165/NRP-1 interaction. Peptidomimetic type inhibitors represent a promising strategy in the treatment of NRP-1-related disorders. Here, we present the synthesis, affinity, enzymatic stability, molecular modeling and in vitro binding evaluation of the branched urea–peptide hybrids, based on our previously reported Lys(hArg)-Dab-Oic-Arg active sequence, where the Lys(hArg) branching has been modified by introducing urea units to replace the peptide bond at various positions. One of the resulting hybrids increased the affinity of the compound for NRP-1 more than 10-fold, while simultaneously improving resistance for proteolytic stability in serum. In addition, ligand binding to NRP-1 induced rapid protein stock exocytotic trafficking to the plasma membrane in breast cancer cells. Examined properties characterize this compound as a good candidate for further development of VEGF165/NRP-1 inhibitors. [ABSTRACT FROM AUTHOR]

Published
2021
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31. Urea-Peptide Hybrids as VEGF-A 165 /NRP-1 Complex Inhibitors with Improved Receptor Affinity and Biological Properties.

Author

Puszko AK, Sosnowski P, Rignault-Bricard R, Hermine O, Hopfgartner G, Pułka-Ziach K, Lepelletier Y, and Misicka A

Subjects
Binding Sites, Cell Line, Tumor, Cells, Cultured, Exocytosis drug effects, Humans, Ligands, Oligopeptides chemistry, Protein Binding drug effects, Urea chemistry, Neuropilin-1 metabolism, Oligopeptides pharmacology, Vascular Endothelial Growth Factor A metabolism
Abstract

Neuropilin-1 (NRP-1), the major co-receptor of vascular endothelial growth factor receptor-2 (VEGFR-2), may also independently act with VEGF-A 165 to stimulate tumour growth and metastasis. Therefore, there is great interest in compounds that can block VEGF-A165/NRP-1 interaction. Peptidomimetic type inhibitors represent a promising strategy in the treatment of NRP-1-related disorders. Here, we present the synthesis, affinity, enzymatic stability, molecular modeling and in vitro binding evaluation of the branched urea-peptide hybrids, based on our previously reported Lys( h Arg)-Dab-Oic-Arg active sequence, where the Lys( h Arg) branching has been modified by introducing urea units to replace the peptide bond at various positions. One of the resulting hybrids increased the affinity of the compound for NRP-1 more than 10-fold, while simultaneously improving resistance for proteolytic stability in serum. In addition, ligand binding to NRP-1 induced rapid protein stock exocytotic trafficking to the plasma membrane in breast cancer cells. Examined properties characterize this compound as a good candidate for further development of VEGF 165 /NRP-1 inhibitors.

Published
2020
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