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92 results on '"Purba, Ambrosius"'

1. Role of Hypoxic Secretome from Mesenchymal Stem Cells in Enhancing Tissue Repair: Regulatory Effects on HIF-1α, VEGF, and Fibroblast in a Sphincterotomy Rat Model.

Author

Gaol, Leecarlo Millano Lumban, Purba, Ambrosius, Diposarosa, Rizki, and Pratiwi, Yuni Susanti

Subjects
LABORATORY rats, MESENCHYMAL stem cells, FECAL incontinence, ANUS, EXTRACELLULAR vesicles
Abstract

Background: Fecal incontinence (FI) is the inability to control bowel movements, resulting in fecal leakage. If left untreated, FI can seriously impact the long-term well-being of individuals affected. Recently, using secretome has become a promising new treatment method. The secretome combines growth factors released outside cells during stem cell development, such as mesenchymal stem cells. It consists of soluble proteins, nucleic acids, fats, and extracellular vesicles, which contribute to different cell processes. The primary aim is to assess the impact of hypoxic secretome administration on accelerating wound healing through the HIF-1α pathway in a post-sphincterotomy rat model. Methods: The study was conducted with two distinct groups of 10 rats each, the control and treatment groups, which were injected with hypoxic secretome at 0.3 mL. The inclusion criteria for the rats were as follows: male gender, belonging to the Sprague-Dawley strain, aged between 12 to 16 weeks, with an average body weight ranging from 240 to 250 grams. Results: There was an increase in HIF-1α gene expression in both groups. The treatment group 37 was significantly higher on day 42 (p = 0.001). VEGF increased significantly in the treatment 38 group on day 42 (p = 0.015). The neovascularization score increased significantly in the treatment 39 group during the first 24 hours (p = 0.004). The fibroblast score increased significantly in the 40 treatment group in the first 24 hours (p = 0.000) and 42 days (p = 0.035). After being given secretome, there was a higher increase in % collagen area and collagen area (μm2) in the treatment group compared to the control group (27,77 vs 11.01) and (419.027,66 vs 186.694,16). Conclusion: The use of hypoxic secretome has a significant effect as a choice for the treatment of anal sphincter injury after sphincterotomy through the HIF-1α-VEGF-Fibroblast pathway. [ABSTRACT FROM AUTHOR]

Published
2024
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7. Measurable training program to improve physical performance: literature review

Author

Fachrul Ihsan, Muhammad, Purba, Ambrosius, Goenawan, Hanna, Satia Graha, Ali, and Womsiwor, Daniel

Subjects
physical components, yoga, mda levels and il-6 levels, Materials Science (miscellaneous), GV557-1198.995, education, Business and International Management, sport, Industrial and Manufacturing Engineering, Sports
Abstract

This study aims to determine the appropriate training program and portion in order to improve the physical performance of athletes.This literature review inquiry was obtained from various PubMed and Google Scholar databases by entering keywords, namely: Sports, Yoga, Basic Physical Components, MDA and IL6. This inquiry reviewed 33 articles published in national and international online journals and have been accredited. The results literature review done on prior studies show basic physical components can be optimally improved through exercises based on the FITT formula, namely interval training, fartlek, weight training, circuit training, plyometrix, rope jump and PNF. In addition, showed that aerobic and anaerobic exercises decreased MDA levels. However, the control group experienced a very significant increase in MDA levels. High-intensity exercise was found able to trigger releasing inflammatory mediators such as TNF-ά and IL-6. Furthermore, some literature showed that long-term yoga practice can reduce MDA levels and IL-6 levels as it as it increases antioxidant levels and anti imflamatory levels. This literature review is expected to provide comprehensive explanation of good and measurable training programs for athletes, coaches and sports institutions. Thereby, athletes’ physical performance can be optimally improved and injuries can be avoided so that athletes get sports achievements.

Published
2021
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9. Expression of TNF-α, IL-1β, and Macrophages in Intermittent Hypobaric Hypoxia Exposure in Post-Tooth Extraction Socket Healing Process in Rats.

Author

Linawati, Linawati, Sitam, Suhardjo, Mulyawan, Wawan, Purba, Ambrosius, Syawqie, Achmad, Handharyani, Ekowati, Yuli Subiakto, and Amaliya, Amaliya

Subjects
GENE expression, HEALING, SPRAGUE Dawley rats, MACROPHAGES, TOOTH socket
Abstract

The aim of this study was to investigate expression of TNF-a, IL-1ß, and macrophages in intermittent hypobaric hypoxia exposure in post tooth extraction socket healing process in rats. Forty-five healthy adult male Sprague Dawley rats were used in this study. Tooth extraction was performed on the maxillary left first molar, then randomly divided into 9 groups, namely the intermittent hypobaric hypoxia (IHH) group which was given exposure to hypobaric hypoxia (HH) for 30 minutes every day in the Hypobaric Chamber at an altitude of 18. 000 feet for 1 time HH, 3 times HH, 5 times HH, and 7 times HH, and the normoxia group that was not exposed to HH, and the control group were terminated on days 0, 1, 3, 5 and 7. Molecular changes in mRNA expression were measured by RT-PCR extracted from socket tissue after rat tooth extraction to evaluate the expression of TNF-a mRNA and IL-1ß mRNA, as well as histological changes by Hematoxylin and Eosin (H&E) staining to evaluate macrophages. Molecular and histological parameters were calculated at the end of each experiment on days 0,1,3,5, and 7 after tooth extraction as repair phase of the socket healing process. In this study, it was found that the expression of TNF-a mRNA, IL-1ß mRNA and the number of macrophages, began to increase significantly after 1 time exposure to HH on day 1, and reached the highest peak after 3 times exposures to HH on day 3, this increase was higher than the control group and the normoxia group, then after 5 times exposures to HH on day 5 there was a decrease, and further decreased after 7 times exposures to HH on day 7, this decrease also occurred faster approaching the control group than the normoxia group. Repeated or intermittent exposure to hypobaric hypoxic conditions induces a protective response, can make cells adapt to hypoxia. Several inflammatory cytokines and inflammatory processes, such as inflammatory cell migration and monocyte differentiation into macrophages, are modulated under hypoxic conditions. TNF-a and IL-1ß are proinflammatory cytokines produced by macrophages in early wounds, and play an important role in inflammation and immune responses in wound healing. The results showed that changes in TNF-a and IL-1ß mRNA expression were in line with changes in the number of macrophages in the socket after tooth extraction under hypobaric hypoxic conditions, and there was a gradual mechanism of cell adaptation under intermittent hypobaric hypoxic conditions which could affect the wound healing process after tooth extraction. [ABSTRACT FROM AUTHOR]

Published
2023

11. Effect of nutmeg extract on the white adipose tissue (WAT) browning process of aging rats

Author

Pratiwi, Yuni Susanti, primary, Siannoto, Melisa, additional, Goenawan, Hanna, additional, Sylviana, Nova, additional, Tarawan, Vita Murniati, additional, Nurhayati, Titing, additional, Setiawan, Iwan, additional, Nugraha, Gaga Irawan, additional, Dwipa, Lazuardhi, additional, Purba, Ambrosius, additional, Supratman, Unang, additional, and Lesmana, Ronny, additional

Published
2022
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27. Effect of nutmeg extract on the white adipose tissue (WAT) browning process of aging rats.

Author

Pratiwi, Yuni Susanti, Siannoto, Melisa, Goenawan, Hanna, Sylviana, Nova, Tarawan, Vita Murniati, Nurhayati, Titing, Setiawan, Iwan, Nugraha, Gaga Irawan, Dwipa, Lazuardhi, Purba, Ambrosius, Supratman, Unang, and Lesmana, Ronny

Subjects
WHITE adipose tissue, WEIGHT loss, NUTMEG tree, BROWN adipose tissue, PEROXISOME proliferator-activated receptors, RATS, AGING
Abstract

The white adipose tissue (WAT) browning process has become one of the promising methods for managing obesity. During this process, WAT is transformed into brown-like adipose tissue, which is also known as beige adipose tissue. The browning process can be activated by several inducers. One of the best candidates is peroxisome proliferator-activated receptor γ (PPARγ) agonist. Nutmeg (Myristica fragrans Houtt) is a natural PPARα/γ partial agonist that is known to contribute to the browning effect. This study aimed to explore the potential effect of nutmeg seed extract (NuSE) on body weight reduction and uncoupling protein (UCP)1, UCP2, UCP3, and peroxisome proliferator-activated receptor gamma coactivator-1 PGC-1α levels in aging rats. Eight male Wistar rats (80 weeks old) were divided into control and treatment groups. Both groups were fed a standard diet, and the treatment group was given 8.1 mg/kg body weight/day of NuSE via oral gavage for 12 weeks. After 12 weeks, the levels of UCP1, UCP2, UCP3, and PGC-1α from both inguinal WAT (iWAT) and interscapular brown adipose tissue (BAT) were examined. We observed that the administration of NuSE has no significant effect to the decreasement of rats body weights (p = 0.464), levels of UCP1 (p = 0.686), UCP2 (p = 0.360), UCP3 (p = 0.076), and PGC-1α (p = 0.200). [ABSTRACT FROM AUTHOR]

Published
2021
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31. Effect of Short-Term Endurance Exercise on COX IV and PGC-1a mRNA Expression Levels in Rat Skeletal Muscle

Author

Sylviana, Nova, primary, Natalia, Christina, additional, Goenawan, Hanna, additional, Pratiwi, Yuni Susanti, additional, Setiawan, Iwan, additional, Tarawan, Vita Murniati, additional, Nurhayati, Titing, additional, Rezano, Andri, additional, Juliati, Juliati, additional, Purba, Ambrosius, additional, Supratman, Unang, additional, and Lesmana, Ronny, additional

Published
2019
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39. Nutmeg Extract Increases Skeletal Muscle Mass in Aging Rats Partly via IGF1-AKT-mTOR Pathway and Inhibition of Autophagy

Author

Pratiwi, Yuni Susanti, primary, Lesmana, Ronny, additional, Goenawan, Hanna, additional, Sylviana, Nova, additional, Setiawan, Iwan, additional, Tarawan, Vita Murniati, additional, Lestari, Keri, additional, Abdulah, Rizky, additional, Dwipa, Lazuardhi, additional, Purba, Ambrosius, additional, and Supratman, Unang, additional

Published
2018
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41. Effect of Swimming Exercise to Cardiac PGC-1 α and HIF-1 α Gene Expression in Mice

Author

Sylviana, Nova, primary, Helja, Niswan, additional, Qolbi, Hadaral Hudanul, additional, Goenawan, Hanna, additional, Lesmana, Ronny, additional, Syamsunarno, Mas Rizky A A, additional, Agustina, Hasrayati, additional, Tiksnadi, Badai Batara, additional, Megantara, Imam, additional, Tarawan, Vita Murniati, additional, Setiawan, Setiawan, additional, Hernowo, Bethy, additional, Supratman, Unang, additional, and Purba, Ambrosius, additional

Published
2018
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45. Roselle (Hibiscus sabdariffa L.) calyces tea improves physical fitness of healthy adults.

Author

Lubis, Leonardo, Dewi, Gita Tiara, Supriyan, Alyadilla Nur Deyata, Aprinaldi, Aprinaldi, Purba, Ambrosius, and Diantini, Ajeng

Subjects
PHYSICAL fitness, ROSELLE, VERTICAL jump, OXYGEN consumption, TEA, HERBAL teas
Abstract

Roselle calyces (Hibiscus sabdariffa L.) is a plant that contains anthocyanin and flavonoids, which function as exogenous antioxidants for the human body to counteract excess oxidative stress. Roselle has anti-hypertensive, anti-cancer and anti-diabetic effects. The present study evaluated roselle to see whether it affects physical fitness. The components of physical fitness include strength, balance, right and left hand grip, vertical jump and VO2max (maximum oxygen consumption). A total of 30 subjects received 200 ml rosella tea for 30 days every morning and evening. Every week, subjects were assessed for physical fitness. Data analysis used paired t and Wilcoxon test according to the normality test results. The results showed significant improvements in strength (from 24.9 to 27.3 kg; P=0.025), balance (from 23.3 to 42.2 sec; P=0.004), right (from 31.8 to 35.1 kg; P<0.0001) and left hand grip (from 29.8 to 31.6 kg; P=0.020), vertical jump (from 38.6 to 41.1 m/sec; P=0.008) and VO2max (from 31.1 to 34.3 ml/kg/min; P=0.014). This demonstrated that roselle significantly improved six parameters of physical fitness and may be used as a supplement to improve physical fitness without severe side effects. [ABSTRACT FROM AUTHOR]

Published
2024
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