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17 results on '"Puppel, Sven Holger"'

1. Characterization and evaluation of gene fusions as a measure of genetic instability and disease prognosis in prostate cancer

Author

Schimmelpfennig, Carolin, Rade, Michael, Füssel, Susanne, Löffler, Dennis, Blumert, Conny, Bertram, Catharina, Borkowetz, Angelika, Otto, Dominik J., Puppel, Sven-Holger, Hönscheid, Pia, Sommer, Ulrich, Baretton, Gustavo B., Köhl, Ulrike, Wirth, Manfred, Thomas, Christian, Horn, Friedemann, Kreuz, Markus, and Reiche, Kristin

Published
2023
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2. ProstaTrend—A Multivariable Prognostic RNA Expression Score for Aggressive Prostate Cancer

Author

Kreuz, Markus, Otto, Dominik J., Fuessel, Susanne, Blumert, Conny, Bertram, Catharina, Bartsch, Sophie, Loeffler, Dennis, Puppel, Sven-Holger, Rade, Michael, Buschmann, Tilo, Christ, Sabina, Erdmann, Kati, Friedrich, Maik, Froehner, Michael, Muders, Michael H., Schreiber, Stephan, Specht, Michael, Toma, Marieta I., Benigni, Fabio, Freschi, Massimo, Gandaglia, Giorgio, Briganti, Alberto, Baretton, Gustavo B., Loeffler, Markus, Hackermüller, Jörg, Reiche, Kristin, Wirth, Manfred, and Horn, Friedemann

Published
2020
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3. TERT expression is associated with metastasis from thin primaries, exhausted CD4+ T cells in melanoma and with DNA repair across cancer entities

Author

Kuhn, Christina Katharina, primary, Meister, Jaroslawna, additional, Kreft, Sophia, additional, Stiller, Mathias, additional, Puppel, Sven-Holger, additional, Zaremba, Anne, additional, Scheffler, Björn, additional, Ullrich, Vivien, additional, Schöneberg, Torsten, additional, Schadendorf, Dirk, additional, and Horn, Susanne, additional

Published
2023
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6. TERTexpression is associated with metastasis from thin primaries, exhausted CD4+ T cells in melanoma and with DNA repair across cancer entities

Author

Kuhn, Christina Katharina, primary, Meister, Jaroslawna, additional, Kreft, Sophia, additional, Stiller, Mathias, additional, Puppel, Sven-Holger, additional, Zaremba, Anne, additional, Scheffler, Björn, additional, Ullrich, Vivien, additional, Schöneberg, Torsten, additional, Schadendorf, Dirk, additional, and Horn, Susanne, additional

Published
2023
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8. NEXT-GENERATION SEQUENCING REVEALS TRANSCRIPT CLUSTERS WITH PROGNOSTIC POTENTIAL FOR PROSTATE CANCER: MP02-03

Author

Horn, Friedemann, Christ-Breulmann, Sabina, Puppel, Sven-Holger, Buschmann, Tilo, Reiche, Kristin, Specht, Michael, Bertram, Catharina, Friedrich, Maik, Binder, Stefanie, Blumert, Conny, Hackermüller, Jörg, Kreuz, Markus, Löffler, Markus, Toma, Marieta I., Muders, Michael, Baretton, Gustavo B., Fröhner, Michael, Füssel, Susanne, and Wirth, Manfred

Published
2016
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9. Deep sequencing and automated histochemistry of human tissue slice cultures improve their usability as preclinical model for cancer research

Author

Haehnel, Susann, Reiche, Kristin, Loeffler, Dennis, Horn, Andreas, Blumert, Conny, Puppel, Sven-Holger, Kaiser, Nicole, Rapp, Felicitas, Rade, Michael, Horn, Friedemann, Meixensberger, Juergen, Bechmann, Ingo, Gaunitz, Frank, Winter, Karsten, and Publica

Subjects
Histocytochemistry, Sequence Analysis, RNA, Gene Expression Profiling, Science, High-Throughput Nucleotide Sequencing, Immunohistochemistry, Article, CNS cancer, Humans, Medicine, Cancer models, Glioblastoma, Transcriptome, ddc:600
Abstract

Scientific reports 9(1), 19961 (2019). doi:10.1038/s41598-019-56509-5, Published by Macmillan Publishers Limited, part of Springer Nature, [London]

Published
2019
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10. Database for queries of melanoma cohorts with molecular and clinical data.

Author

Philip, Manuel, Puppel, Sven Holger, Stiller, Mathias, Paschen, Annette, Roesch, Alexander, Zaremba, Anne, Schadendorf, Dirk, and Horn, Susanne

Subjects
*DATABASES, *MELANOMA, *GENE expression, *IMMUNE checkpoint inhibitors
Abstract

Large genome‐scale studies are deposited in various public sequence repositories. However, their access and analysis can be non‐trivial to infrequent users. Here, we present a new database connecting whole transcriptomes with clinical data for straight‐forward access and analysis of patient‐specific samples. Users can perform association tests of survival and gene expression across different cohorts, identify cell‐type expressions, or correlate the presence of immune cells. In summary, we present a new data hub for bench scientists to perform replication and discovery studies. [ABSTRACT FROM AUTHOR]

Published
2023
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11. The Role of lncRNAs TAPIR-1 and -2 as Diagnostic Markers and Potential Therapeutic Targets in Prostate Cancer

Author

Friedrich, Maik, primary, Wiedemann, Karolin, additional, Reiche, Kristin, additional, Puppel, Sven-Holger, additional, Pfeifer, Gabriele, additional, Zipfel, Ivonne, additional, Binder, Stefanie, additional, Köhl, Ulrike, additional, Müller, Gerd A., additional, Engeland, Kurt, additional, Aigner, Achim, additional, Füssel, Susanne, additional, Fröhner, Michael, additional, Peitzsch, Claudia, additional, Dubrovska, Anna, additional, Rade, Michael, additional, Christ, Sabina, additional, Schreiber, Stephan, additional, Hackermüller, Jörg, additional, Lehmann, Jörg, additional, Toma, Marieta I., additional, Muders, Michael H., additional, Sommer, Ulrich, additional, Baretton, Gustavo B., additional, Wirth, Manfred, additional, and Horn, Friedemann, additional

Published
2020
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13. The Role of lncRNAs TAPIR-1 and -2 as Diagnostic Markers and Potential Therapeutic Targets in Prostate Cancer

Author

Friedrich, Maik, Wiedemann, Karolin, Reiche, Kristin, Puppel, Sven-Holger, Pfeifer, Gabriele, Zipfel, Ivonne, Binder, Stefanie, Köhl, Ulrike, Müller, Gerd A., Engeland, Kurt, Aigner, Achim, Füssel, Susanne, Fröhner, Michael, Peitzsch, Claudia, Dubrovska, Anna, Rade, Michael, Christ, Sabina, Schreiber, Stephan, Hackermüller, Jörg, Lehmann, Jörg, Toma, Marieta I., Muders, Michael H., Sommer, Ulrich, Baretton, Gustavo B., Wirth, Manfred, Horn, Friedemann, Friedrich, Maik, Wiedemann, Karolin, Reiche, Kristin, Puppel, Sven-Holger, Pfeifer, Gabriele, Zipfel, Ivonne, Binder, Stefanie, Köhl, Ulrike, Müller, Gerd A., Engeland, Kurt, Aigner, Achim, Füssel, Susanne, Fröhner, Michael, Peitzsch, Claudia, Dubrovska, Anna, Rade, Michael, Christ, Sabina, Schreiber, Stephan, Hackermüller, Jörg, Lehmann, Jörg, Toma, Marieta I., Muders, Michael H., Sommer, Ulrich, Baretton, Gustavo B., Wirth, Manfred, and Horn, Friedemann

Abstract

In search of new biomarkers suitable for the diagnosis and treatment of prostate cancer, genome-wide transcriptome sequencing was carried out with tissue specimens from 40 prostate cancer (PCa) and 8 benign prostate hyperplasia patients. We identified two intergenic long non-coding transcripts, located in close genomic proximity, which are highly expressed in PCa. Microarray studies on a larger cohort comprising 155 patients showed a profound diagnostic potential of these transcripts (AUC~0.94), which we designated as tumor associated prostate cancer increased lncRNA (TAPIR-1 and -2). To test their therapeutic potential, knockdown experiments with siRNA were carried out. The knockdown caused an increase in the p53/TP53 tumor suppressor protein level followed by downregulation of a large number of cell cycle- and DNA-damage repair key regulators. Furthermore, in radiation therapy resistant tumor cells, the knockdown leads to a renewed sensitization of these cells to radiation treatment. Accordingly, in a preclinical PCa xenograft model in mice, the systemic application of nanoparticles loaded with siRNA targeting TAPIR-1 significantly reduced tumor growth. These findings point to a crucial role of TAPIR-1 and -2 in PCa.

Published
2020

14. uap: Reproducible and Robust HTS Data Analysis

Author

Kämpf, Christoph, primary, Specht, Michael, additional, Scholz, Alexander, additional, Puppel, Sven-Holger, additional, Doose, Gero, additional, Reiche, Kristin, additional, Schor, Jana, additional, and Hackermüller, Jörg, additional

Published
2019
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16. Extracellular vesicle-associated miRNAs in ovarian cancer – design of an integrated NGS-based workflow for the identification of blood-based biomarkers for platinum-resistance.

Author

Kuhlmann, Jan Dominik, Chebouti, Issam, Kimmig, Rainer, Buderath, Paul, Reuter, Michael, Puppel, Sven-Holger, Wimberger, Pauline, and Kasimir-Bauer, Sabine

Subjects
VESICLES (Cytology), MICRORNA, OVARIAN cancer, WORKFLOW, BIOLOGICAL tags
Abstract

Background: Extracellular vesicle (EV)-associated microRNAs (miRNAs) have been suggested as promising biomarkers for blood-based cancer diagnosis. However, one of the major limitations for the use of EVs with diagnostic purpose is the lack of standardized EV-profiling techniques. In this regard, the objective of our study was to design an integrated next-generation sequencing (NGS)-based workflow for analyzing the signature of EV-associated miRNA in the plasma of platinum-resistant ovarian cancer patients. Methods: For EV-extraction, different enrichment methods were compared (ExoQuick vs. exoRNeasy). NGS was performed with the Illumina platform. Results: We established an integrated NGS-based workflow, including EV-enrichment with the ExoQuick system, which resulted in an optimal RNA-yield and consistent small RNA libraries. We applied this workflow in a pilot cohort of clinically documented platinum-sensitive (n=15) vs. platinum-resistant (n=15) ovarian cancer patients, resulting in a panel of mature EV-associated miRNAs (including ovarian cancer associated miR-181a, miR-1908, miR-21, miR-486 and miR-223), which were differentially abundant in the plasma of platinum-resistant patients. Conclusions: This is the first study, analyzing the profile of EV-associated miRNAs in platinum-resistant ovarian cancer patients. We provide rationale to further validate these miRNA candidates in an independent set of patients, in order to characterize their biomarker potential as predictors for platinum-resistance. [ABSTRACT FROM AUTHOR]

Published
2019
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17. MP02-03 NEXT-GENERATION SEQUENCING REVEALS TRANSCRIPT CLUSTERS WITH PROGNOSTIC POTENTIAL FOR PROSTATE CANCER

Author

Horn, Friedemann, primary, Christ-Breulmann, Sabina, additional, Puppel, Sven-Holger, additional, Buschmann, Tilo, additional, Reiche, Kristin, additional, Specht, Michael, additional, Bertram, Catharina, additional, Friedrich, Maik, additional, Binder, Stefanie, additional, Blumert, Conny, additional, Hackermüller, Jörg, additional, Kreuz, Markus, additional, Löffler, Markus, additional, Toma, Marieta I., additional, Muders, Michael, additional, Baretton, Gustavo B., additional, Fröhner, Michael, additional, Füssel, Susanne, additional, and Wirth, Manfred, additional

Published
2016
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