2,105 results on '"Pulmonary Fibrosis physiopathology"'
Search Results
2. The Role of Inflammation and Fibrosis in Interstitial Lung Disease Treatment Decisions.
- Author
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Behr J, Salisbury ML, Walsh SLF, Podolanczuk AJ, Hariri LP, Hunninghake GM, Kolb M, Ryerson CJ, Cottin V, Beasley MB, Corte T, Glanville AR, Adegunsoye A, Hogaboam C, Wuyts WA, Noth I, Oldham JM, Richeldi L, Raghu G, and Wells AU
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- Humans, Pulmonary Fibrosis physiopathology, Pulmonary Fibrosis etiology, Clinical Decision-Making, Lung Diseases, Interstitial physiopathology, Lung Diseases, Interstitial etiology, Inflammation
- Published
- 2024
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3. Use of Race-Specific Equations in Pulmonary Function Tests Impedes Potential Eligibility for Care and Treatment of Pulmonary Fibrosis.
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Adegunsoye A, Bachman WM, Flaherty KR, Li Z, and Gupta S
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- Humans, Male, Female, Middle Aged, Aged, Vital Capacity, United States, Eligibility Determination methods, Respiratory Function Tests, Pulmonary Fibrosis physiopathology, Pulmonary Fibrosis therapy, Pulmonary Fibrosis ethnology, Hispanic or Latino statistics & numerical data, Registries, Patient Selection, White People statistics & numerical data, Black or African American, Lung Diseases, Interstitial physiopathology, Lung Diseases, Interstitial therapy, Lung Diseases, Interstitial ethnology, Lung Diseases, Interstitial diagnosis, Reference Values, Lung Transplantation
- Abstract
Rationale: The use of race-specific reference values to evaluate pulmonary function has long been embedded into clinical practice; however, there is a growing consensus that this practice may be inappropriate and that the use of race-neutral equations should be adopted to improve access to health care. Objectives: To evaluate whether the use of race-neutral equations to assess percent predicted forced vital capacity (FVC%
pred ) impacts eligibility for clinical trials, antifibrotic therapy, and referral for lung transplantation in Black, Hispanic/Latino, and White patients with interstitial lung disease (ILD). Methods: FVC%pred values for patients from the Pulmonary Fibrosis Foundation Patient Registry were calculated using race-specific (Hankinson and colleagues, 1999), race-agnostic (Global Lung Function Initiative [GLI]-2012), and race-neutral (GLI-2022 or GLI-Global) equations. Eligibility for ILD clinical trials (FVC%pred >45% and <90%), antifibrotic therapy (FVC%pred >55% and <82%), and lung transplantation referral (FVC%pred <70%) based on GLI-2022 and GLI-2012 equations were compared with those based on the Hankinson 1999 equation. Results: Baseline characteristics were available for 1,882 patients (Black, n = 104; Hispanic/Latino, n = 103; White, n = 1,675), and outcomes were evaluated in 1,531 patients with FVC%pred within ±90 days of registry enrollment (Black, n = 78; Hispanic/Latino, n = 72; White, n = 1,381). Black patients were younger at the time of consent and more likely to be female compared with Hispanic/Latino or White patients. Compared with GLI-2022, the Hankinson 1999 equation misclassified 22% of Black patients, 14% of Hispanic/Latino patients, and 12% of White patients for ILD clinical trial eligibility; 21% of Black patients, 17% of Hispanic/Latino patients, and 19% of White patients for antifibrotic therapy eligibility; and 6% of Black patients, 14% of Hispanic/Latino patients, and 12% of White patients for lung transplantation referral. Similar trends were observed when comparing the GLI-2012 and Hankinson 1999 equations. Conclusions: Misclassification of patients for critical interventions is highly prevalent when using the Hankinson 1999 equation and highlights the need to consider adopting the race-neutral GLI-2022 equation for enhanced accuracy and more equitable representation in pulmonary health care. Our results make a compelling case for reevaluating the use of race as a physiological variable and emphasize the pressing need for continuous innovation to ensure equal and optimal care for all patients regardless of their race or ethnicity. Clinical trial registered with www.clinicaltrials.gov (NCT02758808).- Published
- 2024
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4. Safety and feasibility of pulmonary rehabilitation in patients hospitalized with post-COVID-19 fibrosis: A feasibility study.
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Nair SP, Augustine A, Panchabhai C, Patil S, Parmar K, and Panhale VP
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- Humans, Male, Female, Middle Aged, Prospective Studies, Hospitalization, SARS-CoV-2, Aged, Pulmonary Fibrosis rehabilitation, Pulmonary Fibrosis physiopathology, Breathing Exercises methods, Adult, Pandemics, Feasibility Studies, COVID-19 complications, COVID-19 rehabilitation
- Abstract
Background: Emerging data suggest a spectrum of pulmonary complications from COVID-19, ranging from dyspnea to difficult ventilator weaning and fibrotic lung damage. Prolonged hospitalization is known to significantly affect activity levels, impair muscle strength and reduce cardiopulmonary endurance., Objective: To assess the feasibility and safety of inpatient pulmonary rehabilitation (PR) and to explore effects on functional capacity, physical performance, fatigue levels, and functional status., Design: A prospective feasibility study., Setting: Inpatient unit of a tertiary care hospital., Participants: Twenty-five hospitalized patients diagnosed with post-COVID-19 fibrosis referred for PR., Intervention: Individualized PR intervention including breathing exercises, positioning, strengthening, functional training, and ambulation twice a day for 6 days a week., Outcome Measures: One-minute sit-to-stand test (STST), Short Physical Performance Battery (SPPB), Fatigue Assessment Scale (FAS), and Post-COVID-19 Functional Status Scale (PCFS)., Results: Twenty-five participants (19 males, 6 females) with a mean age of 54.2 ± 13.4 years were enrolled. Sixteen completed the two-point assessment after undergoing in-patient PR of mean duration 14.8 ± 9 days. PR led to a significant improvement in all functional outcomes that is, STST (from 7.1 ± 4.3 repetitions to 14.2 ± 2.1 repetitions, SPPB (from 5 ± 2.8 to 9.4 ± 1.5), FAS (from 33.3 ± 10.8 to 25.8 ± 4.7) at the p ≤ .001, and PCFS (from 3.6 ± 0.9 to 2.9 ± 1.2, p ≤ .05)., Conclusion: Early initiation of PR for hospitalized patients with COVID-19 fibrosis was safe, well tolerated, and feasible and may improve functional status., (© 2023 American Academy of Physical Medicine and Rehabilitation.)
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- 2024
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5. Simultaneous Positron Emission Tomography and Molecular Magnetic Resonance Imaging of Cardiopulmonary Fibrosis in a Mouse Model of Left Ventricular Dysfunction.
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Moon BF, Zhou IY, Ning Y, Chen YI, Le Fur M, Shuvaev S, Akam EA, Ma H, Solsona CM, Weigand-Whittier J, Rotile N, Hariri LP, Drummond M, Boice AT, Zygmont SE, Sharma Y, Warburton RR, Martin GL, Blanton RM, Fanburg BL, Hill NS, Caravan P, and Penumatsa KC
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- Animals, Mice, Myocardium pathology, Myocardium metabolism, Pulmonary Fibrosis diagnostic imaging, Pulmonary Fibrosis physiopathology, Pulmonary Fibrosis metabolism, Pulmonary Fibrosis etiology, Ventricular Function, Left, Male, Lung diagnostic imaging, Lung pathology, Lung physiopathology, Lung metabolism, Multimodal Imaging methods, Collagen metabolism, Ventricular Remodeling, Lysine analogs & derivatives, Positron-Emission Tomography methods, Disease Models, Animal, Fibrosis, Ventricular Dysfunction, Left diagnostic imaging, Ventricular Dysfunction, Left physiopathology, Ventricular Dysfunction, Left etiology, Ventricular Dysfunction, Left metabolism, Magnetic Resonance Imaging methods
- Abstract
Background: Aging-associated left ventricular dysfunction promotes cardiopulmonary fibrogenic remodeling, Group 2 pulmonary hypertension (PH), and right ventricular failure. At the time of diagnosis, cardiac function has declined, and cardiopulmonary fibrosis has often developed. Here, we sought to develop a molecular positron emission tomography (PET)-magnetic resonance imaging (MRI) protocol to detect both cardiopulmonary fibrosis and fibrotic disease activity in a left ventricular dysfunction model., Methods and Results: Left ventricular dysfunction was induced by transverse aortic constriction (TAC) in 6-month-old senescence-accelerated prone mice, a subset of mice that received sham surgery. Three weeks after surgery, mice underwent simultaneous PET-MRI at 4.7 T. Collagen-targeted PET and fibrogenesis magnetic resonance (MR) probes were intravenously administered. PET signal was computed as myocardium- or lung-to-muscle ratio. Percent signal intensity increase and Δ lung-to-muscle ratio were computed from the pre-/postinjection magnetic resonance images. Elevated allysine in the heart ( P =0.02) and lungs ( P =0.17) of TAC mice corresponded to an increase in myocardial magnetic resonance imaging percent signal intensity increase ( P <0.0001) and Δlung-to-muscle ratio ( P <0.0001). Hydroxyproline in the heart ( P <0.0001) and lungs ( P <0.01) were elevated in TAC mice, which corresponded to an increase in heart (myocardium-to-muscle ratio, P =0.02) and lung (lung-to-muscle ratio, P <0.001) PET measurements. Pressure-volume loop and echocardiography demonstrated adverse left ventricular remodeling, function, and increased right ventricular systolic pressure in TAC mice., Conclusions: Administration of collagen-targeted PET and allysine-targeted MR probes led to elevated PET-magnetic resonance imaging signals in the myocardium and lungs of TAC mice. The study demonstrates the potential to detect fibrosis and fibrogenesis in cardiopulmonary disease through a dual molecular PET-magnetic resonance imaging protocol.
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- 2024
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6. Evaluating the impact of type 2 diabetes mellitus on pulmonary vascular function and the development of pulmonary fibrosis.
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Mzimela N, Dimba N, Sosibo A, and Khathi A
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- Humans, Prediabetic State complications, Prediabetic State physiopathology, Prediabetic State metabolism, Animals, Lung physiopathology, Lung pathology, Diabetes Mellitus, Type 2 complications, Diabetes Mellitus, Type 2 physiopathology, Pulmonary Fibrosis physiopathology
- Abstract
The increasing prevalence of type 2 diabetes mellitus (T2DM) is a significant worldwide health concern caused by sedentary lifestyles and unhealthy diets. Beyond glycemic control, T2DM impacts multiple organ systems, leading to various complications. While traditionally associated with cardiovascular and microvascular complications, emerging evidence indicates significant effects on pulmonary health. Pulmonary vascular dysfunction and fibrosis, characterized by alterations in vascular tone and excessive extracellular matrix deposition, are increasingly recognized in individuals with T2DM. The onset of T2DM is often preceded by prediabetes, an intermediate hyperglycemic state that is associated with increased diabetes and cardiovascular disease risk. This review explores the relationship between T2DM, pulmonary vascular dysfunction and pulmonary fibrosis, with a focus on potential links with prediabetes. Pulmonary vascular function, including the roles of nitric oxide (NO), prostacyclin (PGI2), endothelin-1 (ET-1), thromboxane A2 (TxA2) and thrombospondin-1 (THBS1), is discussed in the context of T2DM and prediabetes. Mechanisms linking T2DM to pulmonary fibrosis, such as oxidative stress, dysregulated fibrotic signaling, and chronic inflammation, are explained. The impact of prediabetes on pulmonary health, including endothelial dysfunction, oxidative stress, and dysregulated vasoactive mediators, is highlighted. Early detection and intervention during the prediabetic stage may reduce respiratory complications associated with T2DM, emphasizing the importance of management strategies targeting blood glucose regulation and vascular health. More research that looks into the mechanisms underlying pulmonary complications in T2DM and prediabetes is needed., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Mzimela, Dimba, Sosibo and Khathi.)
- Published
- 2024
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7. Validation of a Dyspnea Visual Analog Scale in Fibrotic Interstitial Lung Disease.
- Author
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Bevanda L, Mok V, Lin K, Assayag D, Fisher JH, Johannson KA, Khalil N, Kolb M, Manganas H, Marcoux V, Sadatsafavi M, Wong AW, and Ryerson CJ
- Subjects
- Humans, Male, Female, Middle Aged, Aged, Prospective Studies, Severity of Illness Index, Prognosis, Registries, Surveys and Questionnaires, Vital Capacity, Pulmonary Fibrosis complications, Pulmonary Fibrosis physiopathology, Dyspnea etiology, Dyspnea diagnosis, Lung Diseases, Interstitial complications, Lung Diseases, Interstitial physiopathology, Lung Diseases, Interstitial diagnosis, Quality of Life, Visual Analog Scale
- Abstract
Rationale: A visual analog scale (VAS) is a simple and easily administered tool for measuring the impact of disease; however, little is known about the use of a dyspnea VAS in interstitial lung disease (ILD). Objectives: To validate the use of a dyspnea VAS in a large and heterogeneous cohort of patients with fibrotic ILD, including its minimal clinically important difference (MCID), responsiveness to change, and prognostic significance. Methods: Patients with fibrotic ILD were identified from a large prospective registry. The validity of a 100-mm dyspnea VAS was assessed by testing its correlation in change score with other measures of ILD severity, including the University of California San Diego Shortness of Breath Questionnaire, the King's Brief Interstitial Lung Disease quality of life questionnaire Breathlessness and Activities Domain, the European Quality of Life VAS, forced vital capacity, and diffusing capacity of the lung for carbon monoxide. The responsiveness of the dyspnea VAS was qualitatively confirmed on the basis of there being an observable difference in the change in dyspnea VAS across tertiles of change in anchor variables. The MCID in dyspnea VAS was calculated using both anchor (linear regression) and distribution (one-half standard deviation) approaches, with anchors including the above variables that had a correlation with dyspnea VAS (| r | ≥ 0.30). The association of dyspnea VAS with time to death or transplant was determined. Results: The cohort included 826 patients with fibrotic ILD, including 127 patients with follow-up measurements at 6 months. The mean baseline dyspnea VAS was 53 ± 24 mm. Dyspnea VAS change scores were moderately correlated with the University of California San Diego Shortness of Breath Questionnaire (| r | = 0.55) and the King's Brief Interstitial Lung Disease quality of life questionnaire Breathlessness and Activities Domain (| r | = 0.44) and weakly correlated with the European Quality of Life VAS (| r | = 0.19), forced vital capacity percent predicted (| r | = 0.21), and diffusing capacity of the lung for carbon monoxide percent predicted (| r | = 0.05). The MCID was 2.7 to 4.5 using the more reliable anchor-based methods and 12.0 based on distribution-based methods. Dyspnea VAS was associated with time to death or transplant in unadjusted models and after adjustment for age and sex (hazard ratios, 1.16 and 1.15, respectively; P < 0.05 for both). Conclusions: This study provides support for the use of the dyspnea VAS in patients with fibrotic ILD, with an estimated anchor-based MCID of 5 mm.
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- 2024
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8. Repetitive invasive lung function maneuvers do not accentuate experimental fibrosis in mice.
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Röpke T, Aschenbrenner F, Knudsen L, Welte T, Kolb M, and Maus UA
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- Animals, Mice, Lung physiopathology, Lung pathology, Pulmonary Fibrosis physiopathology, Pulmonary Fibrosis pathology, Mice, Inbred C57BL, Male, Transforming Growth Factor beta1 metabolism, Idiopathic Pulmonary Fibrosis physiopathology, Idiopathic Pulmonary Fibrosis pathology, Respiratory Function Tests, Disease Models, Animal
- Abstract
Assessment of lung function is an important clinical tool for the diagnosis and monitoring of chronic lung diseases, including idiopathic pulmonary fibrosis (IPF). In mice, lung function maneuvers use algorithm-based ventilation strategies including forced oscillation technique (FOT), negative pressure-driven forced expiratory (NPFE) and pressure-volume (PV) maneuvers via the FlexiVent system. This lung function test (LFT) is usually performed as end-point measurement only, requiring several mice for each time point to be analyzed. Repetitive lung function maneuvers would allow monitoring of a disease process within the same individual while reducing the numbers of laboratory animals. However, its feasibility in mice and impact on developing lung fibrosis has not been studied so far. Using orotracheal cannulation without surgical exposure of the trachea, we examined the tolerability to repetitive lung function maneuvers (up to four times) in one and the same mouse, both under healthy conditions and in a model of AdTGF-β1 induced lung fibrosis. In essence, we found that repetitive invasive lung function maneuvers were well tolerated and did not accentuate experimental lung fibrosis in mice. This study contributes to the 3R principle aiming to reduce the numbers of experimental animals used in biomedical research., (© 2024. The Author(s).)
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- 2024
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9. Lysophosphatidic acid receptor 1 inhibition: a potential treatment target for pulmonary fibrosis.
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Volkmann ER, Denton CP, Kolb M, Wijsenbeek-Lourens MS, Emson C, Hudson K, Amatucci AJ, Distler O, Allanore Y, and Khanna D
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- Humans, Animals, Molecular Targeted Therapy, Lung drug effects, Lung physiopathology, Lung metabolism, Antifibrotic Agents therapeutic use, Lysophospholipids metabolism, Treatment Outcome, Pulmonary Fibrosis drug therapy, Pulmonary Fibrosis metabolism, Pulmonary Fibrosis physiopathology, Phosphodiesterase Inhibitors therapeutic use, Receptors, Lysophosphatidic Acid antagonists & inhibitors, Receptors, Lysophosphatidic Acid metabolism, Signal Transduction drug effects, Phosphoric Diester Hydrolases metabolism, Idiopathic Pulmonary Fibrosis drug therapy, Idiopathic Pulmonary Fibrosis metabolism
- Abstract
Lysophosphatidic acid (LPA)-mediated activation of LPA receptor 1 (LPAR1) contributes to the pathophysiology of fibrotic diseases such as idiopathic pulmonary fibrosis (IPF) and systemic sclerosis (SSc). These diseases are associated with high morbidity and mortality despite current treatment options. The LPA-producing enzyme autotaxin (ATX) and LPAR1 activation contribute to inflammation and mechanisms underlying fibrosis in preclinical fibrotic models. Additionally, elevated levels of LPA have been detected in bronchoalveolar lavage fluid from patients with IPF and in serum from patients with SSc. Thus, ATX and LPAR1 have gained considerable interest as pharmaceutical targets to combat fibrotic disease and inhibitors of these targets have been investigated in clinical trials for IPF and SSc. The goals of this review are to summarise the current literature on ATX and LPAR1 signalling in pulmonary fibrosis and to help differentiate the novel inhibitors in development. The mechanisms of action of ATX and LPAR1 inhibitors are described and preclinical studies and clinical trials of these agents are outlined. Because of their contribution to numerous physiologic events underlying fibrotic disease, ATX and LPAR1 inhibition presents a promising therapeutic strategy for IPF, SSc and other fibrotic diseases that may fulfil unmet needs of the current standard of care., Competing Interests: Conflict of interest: E.R. Volkmann has received consulting fees from Boehringer Ingelheim, CSL Behring, GSK and Roche; speaking fees (unbranded disease state lectures) from Boehringer Ingelheim; and grant support from Boehringer Ingelheim, Corbus, Forbius, Horizon Therapeutics (now Amgen, Inc), Kadmon and Prometheus. C.P. Denton has received research grants to his institution from Arxx Therapeutics, GSK, Horizon (now Amgen, Inc) and Servier; and consulting fees from Acceleron, Arxx Therapeutics, Bayer, Boehringer Ingelheim, CSL Behring, Galapagos, GSK, Janssen, Lilly, Roche and Sanofi. M. Kolb reports grants from Boehringer Ingelheim, the Canadian Institute for Health Research, Structure Therapeutics and United Therapeutics; and personal fees from AbbVie, Algernon, Bellerophon, Boehringer Ingelheim, Cipla, CSL Behring, DevPro Biopharma, European Respiratory Journal, Horizon (now Amgen, Inc), LabCorp, Nitto Denko, Pieris, Roche, Structure Therapeutics and United Therapeutics. M.S. Wijsenbeek-Lourens reports grants to her institution from AstraZeneca/Daiichi-Sankyo, Boehringer Ingelheim, the Dutch Lung Foundation, the Dutch Pulmonary Fibrosis Patients Association, Hoffmann-La Roche, The Netherlands Organisation for Health Research and Development, Sarcoidosis.nl and the Thorax Foundation; and consultancy fees paid to her institution from AstraZeneca, Boehringer Ingelheim, BMS, CSL Behring, Galapagos, Galecto, Hoffmann-La Roche, Horizon Therapeutics (now Amgen, Inc), Kinevant Sciences, Molecure, NeRRe, Novartis, PureTech, Thyron, Trevi and Vicore. C. Emson and K. Hudson are employees of Horizon Therapeutics (now Amgen, Inc) and own stock. A.J. Amatucci was employed at Horizon Therapeutics (now Amgen, Inc) during development of this manuscript. O. Distler has/had a consultancy relationship with and/or has received research funding from and/or has served as a speaker for the following companies in the area of potential treatments for systemic sclerosis and its complications in the last 3 calendar years: 4P-Pharma, AbbVie, Acceleron, Alcimed, Altavant, Amgen, AnaMar, Argenx, Arxx, AstraZeneca, Blade, Bayer, Boehringer Ingelheim, Corbus, CSL Behring, Galderma, Galapagos, Glenmark, Gossamer, Horizon, Janssen, Kymera, Lupin, Medscape, Merck, Miltenyi Biotec, Mitsubishi Tanabe, Novartis, Orion, Prometheus, Redxpharma, Roivant, Topadur and UCB; has a patent issued “mir-29 for the treatment of systemic sclerosis” (US8247389, EP2331143); and is co-founder of CITUS AG. Y. Allanore has received consulting fees from AbbVie, AstraZeneca, Boehringer Ingelheim, Janssen, Galderma, Medsenic and Prometheus. D. Khanna has received consulting fees from AbbVie, Amgen, AstraZeneca, Boehringer Ingelheim, Chemomab, CSL Behring, GSK, Merck, Novartis, Prometheus, Roche and UCB; and grant support from BMS, Boehringer Ingelheim, Horizon Therapeutics (now Amgen, Inc), Pfizer and Prometheus., (Copyright ©The authors 2024.)
- Published
- 2024
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10. Stress-strain curve and elastic behavior of the fibrotic lung with usual interstitial pneumonia pattern during protective mechanical ventilation.
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Tonelli R, Rizzoni R, Grasso S, Cortegiani A, Ball L, Samarelli AV, Fantini R, Bruzzi G, Tabbì L, Cerri S, Manicardi L, Andrisani D, Gozzi F, Castaniere I, Smit MR, Paulus F, Bos LDJ, Clini E, and Marchioni A
- Subjects
- Humans, Male, Female, Middle Aged, Aged, Elasticity, Ventilator-Induced Lung Injury physiopathology, Pulmonary Fibrosis physiopathology, Pulmonary Fibrosis metabolism, Respiratory Mechanics physiology, Stress, Mechanical, Lung Diseases, Interstitial physiopathology, Models, Theoretical, Respiration, Artificial adverse effects, Respiratory Distress Syndrome physiopathology, Lung physiopathology, Lung pathology
- Abstract
Patients with acute exacerbation of lung fibrosis with usual interstitial pneumonia (EUIP) pattern are at increased risk for ventilator-induced lung injury (VILI) and mortality when exposed to mechanical ventilation (MV). Yet, lack of a mechanical model describing UIP-lung deformation during MV represents a research gap. Aim of this study was to develop a constitutive mathematical model for UIP-lung deformation during lung protective MV based on the stress-strain behavior and the specific elastance of patients with EUIP as compared to that of acute respiratory distress syndrome (ARDS) and healthy lung. Partitioned lung and chest wall mechanics were assessed for patients with EUIP and primary ARDS (1:1 matched based on body mass index and PaO
2 /FiO2 ratio) during a PEEP trial performed within 24 h from intubation. Patient's stress-strain curve and the lung specific elastance were computed and compared with those of healthy lungs, derived from literature. Respiratory mechanics were used to fit a novel mathematical model of the lung describing mechanical-inflation-induced lung parenchyma deformation, differentiating the contributions of elastin and collagen, the main components of lung extracellular matrix. Five patients with EUIP and 5 matched with primary ARDS were included and analyzed. Global strain was not different at low PEEP between the groups. Overall specific elastance was significantly higher in EUIP as compared to ARDS (28.9 [22.8-33.2] cmH2 O versus 11.4 [10.3-14.6] cmH2 O, respectively). Compared to ARDS and healthy lung, the stress/strain curve of EUIP showed a steeper increase, crossing the VILI threshold stress risk for strain values greater than 0.55. The contribution of elastin was prevalent at lower strains, while the contribution of collagen was prevalent at large strains. The stress/strain curve for collagen showed an upward shift passing from ARDS and healthy lungs to EUIP lungs. During MV, patients with EUIP showed different respiratory mechanics, stress-strain curve and specific elastance as compared to ARDS patients and healthy subjects and may experience VILI even when protective MV is applied. According to our mathematical model of lung deformation during mechanical inflation, the elastic response of UIP-lung is peculiar and different from ARDS. Our data suggest that patients with EUIP experience VILI with ventilatory setting that are lung-protective for patients with ARDS., (© 2024. The Author(s).)- Published
- 2024
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11. Antifibrotic therapy in progressive pulmonary fibrosis: a review of recent advances.
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Naqvi M, Hannah J, Lawrence A, Myall K, West A, and Chaudhuri N
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- Humans, Vital Capacity, Lung Diseases, Interstitial physiopathology, Lung Diseases, Interstitial diagnosis, Lung Diseases, Interstitial therapy, Lung Diseases, Interstitial drug therapy, Treatment Outcome, Disease Progression, Pulmonary Fibrosis physiopathology, Pulmonary Fibrosis therapy, Pulmonary Fibrosis drug therapy, Pulmonary Fibrosis diagnosis, Antifibrotic Agents therapeutic use
- Abstract
Introduction: Progressive pulmonary fibrosis (PPF) is a manifestation of a heterogenous group of underlying interstitial lung disease (ILD) diagnoses, defined as non-idiopathic pulmonary fibrosis (IPF) progressive fibrotic ILD meeting at least two of the following criteria in the previous 12 months: worsening respiratory symptoms, absolute decline in forced vital capacity (FVC) more than or equal to 5% and/or absolute decline in diffusing capacity for carbon monoxide (DLCO) more than or equal to 10% and/or radiological progression., Areas Covered: The authors subjectively reviewed a synthesis of literature from PubMed to identify recent advances in the diagnosis and characterisation of PPF, treatment recommendations, and management challenges. This review provides a comprehensive summary of recent advances and highlights future directions for the diagnosis, management, and treatment of PPF., Expert Opinion: Recent advances in defining the criteria for PPF diagnosis and licensing of treatment are likely to support further characterisation of the PPF patient population and improve our understanding of prevalence. The diagnosis of PPF remains challenging with the need for a specialised ILD multidisciplinary team (MDT) approach. The evidence base supports the use of immunomodulatory therapy to treat inflammatory ILDs and antifibrotic therapy where PPF develops. Treatment needs to be tailored to the specific underlying disease and determined on a case-by-case basis.
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- 2024
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12. Pursuing Clinical Predictors and Biomarkers for Progression in ILD: Analysis of the Pulmonary Fibrosis Foundation (PFF) Registry.
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Chang SE, Jia G, Gao X, Schiffman C, Gupta S, Wolters P, and Neighbors M
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- Humans, Male, Female, Middle Aged, Vital Capacity, Aged, Pulmonary Fibrosis physiopathology, Pulmonary Fibrosis diagnosis, Pulmonary Surfactant-Associated Protein D blood, Lung physiopathology, Predictive Value of Tests, Chitinase-3-Like Protein 1 blood, Chemokines, CC, Osteopontin, Receptor for Advanced Glycation End Products blood, Idiopathic Pulmonary Fibrosis physiopathology, Idiopathic Pulmonary Fibrosis diagnosis, Disease Progression, Biomarkers, Registries, Lung Diseases, Interstitial physiopathology, Lung Diseases, Interstitial diagnosis
- Abstract
Introduction: Pulmonary fibrosis is a characteristic of various interstitial lung diseases (ILDs) with differing etiologies. Clinical trials in progressive pulmonary fibrosis (PPF) enroll patients based on previously described clinical criteria for past progression, which include a clinical practice guideline for PPF classification and inclusion criteria from the INBUILD trial. In this study, we compared the ability of past FVC (forced vital capacity) progression and baseline biomarker levels to predict future progression in a cohort of patients from the PFF Patient Registry., Methods: Biomarkers previously associated with pathobiology and/or progression in pulmonary fibrosis were selected to reflect cellular senescence (telomere length), pulmonary epithelium (SP-D, RAGE), myeloid activation (CXCL13, YKL40, CCL18, OPN) and fibroblast activation (POSTN, COMP, PROC3)., Results: PFF or INBUILD-like clinical criteria was used to separate patients into past progressor and non-past progressor groups, and neither clinical criterion appeared to enrich for patients with greater future lung function decline. All baseline biomarkers measured were differentially expressed in patient groups compared to healthy controls. Baseline levels of SP-D and POSTN showed the highest correlations with FVC slope over one year, though correlations were low., Conclusions: Our findings provide further evidence that prior decline in lung function may not predict future disease progression for ILD patients, and elevate the need for molecular definitions of a progressive phenotype. Across ILD subtypes, certain shared pathobiologies may be present based on the molecular profile of certain biomarker groups observed. In particular, SP-D may be a common marker of pulmonary injury and future lung function decline across ILDs., (© 2024. The Author(s).)
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- 2024
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13. Proportion and predictors of FVC decline in patients with interstitial lung disease.
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Macmurdo MG, Ji X, Pimple P, Olson AL, Milinovich A, Martyn-Dow B, Pande A, Zajichek A, Bauman J, Bender S, Conoscenti C, Sugano D, Kattan MW, and Culver DA
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- Humans, Male, Female, Retrospective Studies, Vital Capacity physiology, Middle Aged, Aged, Risk Factors, Pulmonary Fibrosis physiopathology, Pulmonary Fibrosis complications, Pulmonary Fibrosis epidemiology, Incidence, Lung Diseases, Interstitial physiopathology, Lung Diseases, Interstitial epidemiology, Lung Diseases, Interstitial complications, Disease Progression
- Abstract
Rationale: The proportion of patients who develop progressive pulmonary fibrosis (PPF), along with risk factors for progression remain poorly understood., Objectives: To examine factors associated with an increased risk of developing PPF among patients at a referral center., Methods: We identified patients with a diagnosis of interstitial lung disease (ILD) seen within the Cleveland Clinic Health System. Utilizing a retrospective observational approach we estimated the risk of developing progression by diagnosis group and identified key clinical predictors using the FVC component of both the original progressive fibrotic interstitial lung disease (PFILD) and the proposed PPF (ATS) criteria., Results: We identified 5934 patients with a diagnosis of ILD. The cumulative incidence of progression over the 24 months was similar when assessed with the PFILD and PPF criteria (33.1 % and 37.9 % respectively). Of those who met the ATS criteria, 9.5 % did not meet the PFILD criteria. Conversely, 4.3 % of patients who met PFILD thresholds did not achieve the 5 % absolute FVC decline criteria. Significant differences in the rate of progression were seen based on underlying diagnosis. Steroid therapy (HR 1.46, CI 1.31-1.62) was associated with an increased risk of progressive fibrosis by both PFILD and PPF criteria., Conclusion: Regardless of the definition used, the cumulative incidence of progressive disease is high in patients with ILD in the 24 months following diagnosis. Some differences are seen in the risk of progression when assessed by PFILD and PPF criteria. Further work is needed to identify modifiable risk factors for the development of progressive fibrosis., Competing Interests: Declaration of competing interest The study is funded by Boehringer Ingelheim Pharmaceuticals, Inc (BIPI). The authors meet criteria for authorship as recommended by the International Committee of Medical Journal Editors (ICMJE). Maeve Macmurdo, Xinge Ji, Alex Milinovich, Blaine Martyn-Dow, Aman Pande, Alex Zajichek, Janine Bauman, David Sugano, Michael W. Kattan and Daniel A. Culver are employed by the Cleveland Clinic which received research support from BIPI to collaborate on this project. Pratik Pimple, Amy L. Olson, Shaun Bender, and Craig Conoscenti are employees of BIPI. Daniel Culver received honoraria from BIPI for his participation in a Steering Committee, from Pliant for his participation in an Adjudication Committee and coverage for travel costs from Roche. All other members of the Cleveland Clinic study team cited no other disclosures., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
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- 2024
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14. Interstitial Lung Disease and Progressive Pulmonary Fibrosis: a World Trade Center Cohort 20-Year Longitudinal Study.
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Cleven KL, Zeig-Owens R, Mueller AK, Vaeth B, Hall CB, Choi J, Goldfarb DG, Schecter DE, Weiden MD, Nolan A, Salzman SH, Jaber N, Cohen HW, and Prezant DJ
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- Humans, Longitudinal Studies, Male, Middle Aged, Female, Incidence, Vital Capacity, Adult, Prevalence, Risk Factors, New York City epidemiology, Gastroesophageal Reflux epidemiology, Occupational Exposure adverse effects, Smoking adverse effects, Smoking epidemiology, Aged, Time Factors, Emergency Responders statistics & numerical data, September 11 Terrorist Attacks, Lung Diseases, Interstitial epidemiology, Lung Diseases, Interstitial physiopathology, Disease Progression, Pulmonary Fibrosis epidemiology, Pulmonary Fibrosis physiopathology
- Abstract
Purpose: World Trade Center (WTC) exposure is associated with obstructive airway diseases and sarcoidosis. There is limited research regarding the incidence and progression of non-sarcoidosis interstitial lung diseases (ILD) after WTC-exposure. ILD encompasses parenchymal diseases which may lead to progressive pulmonary fibrosis (PPF). We used the Fire Department of the City of New York's (FDNY's) WTC Health Program cohort to estimate ILD incidence and progression., Methods: This longitudinal study included 14,525 responders without ILD prior to 9/11/2001. ILD incidence and prevalence were estimated and standardized to the US 2014 population. Poisson regression modeled risk factors, including WTC-exposure and forced vital capacity (FVC), associated with ILD. Follow-up time ended at the earliest of incident diagnosis, end of study period/case ascertainment, transplant or death., Results: ILD developed in 80/14,525 FDNY WTC responders. Age, smoking, and gastroesophageal reflux disease (GERD) prior to diagnosis were associated with incident ILD, though FVC was not. PPF developed in 40/80 ILD cases. Among the 80 cases, the average follow-up time after ILD diagnosis was 8.5 years with the majority of deaths occurring among those with PPF (PPF: n = 13; ILD without PPF: n = 6)., Conclusions: The prevalence of post-9/11 ILD was more than two-fold greater than the general population. An exposure-response gradient could not be demonstrated. Half the ILD cases developed PPF, higher than previously reported. Age, smoking, and GERD were risk factors for ILD and PPF, while lung function was not. This may indicate that lung function measured after respirable exposures would not identify those at risk for ILD or PPF., (© 2024. The Author(s).)
- Published
- 2024
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15. Do Ultrafine Particles Carry Any Weight When It Comes to Progression of Pulmonary Fibrosis?
- Author
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Frampton MW
- Subjects
- Humans, Particle Size, Disease Progression, Pulmonary Fibrosis physiopathology, Particulate Matter adverse effects
- Published
- 2024
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16. Key learnings from the INBUILD trial in patients with progressive pulmonary fibrosis.
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Mira-Avendano I and Kaye M
- Subjects
- Humans, Vital Capacity, Severity of Illness Index, Treatment Outcome, Randomized Controlled Trials as Topic, Glucocorticoids administration & dosage, Glucocorticoids adverse effects, Glucocorticoids therapeutic use, Lung drug effects, Lung physiopathology, Lung diagnostic imaging, Lung Diseases, Interstitial drug therapy, Lung Diseases, Interstitial physiopathology, Lung Diseases, Interstitial diagnosis, Idiopathic Pulmonary Fibrosis drug therapy, Idiopathic Pulmonary Fibrosis physiopathology, Idiopathic Pulmonary Fibrosis diagnosis, Male, Protein Kinase Inhibitors adverse effects, Protein Kinase Inhibitors therapeutic use, Protein Kinase Inhibitors administration & dosage, Indoles adverse effects, Indoles therapeutic use, Indoles administration & dosage, Disease Progression, Pulmonary Fibrosis drug therapy, Pulmonary Fibrosis physiopathology, Tomography, X-Ray Computed
- Abstract
In a patient with interstitial lung disease (ILD) of known or unknown etiology other than idiopathic pulmonary fibrosis (IPF), progressive pulmonary fibrosis (PPF) is defined by worsening lung fibrosis on high-resolution computed tomography (HRCT), decline in lung function, and/or deterioration in symptoms. The INBUILD trial involved 663 patients with PPF who were randomized to receive nintedanib or placebo. The median exposure to trial medication was approximately 19 months. The INBUILD trial provided valuable learnings about the course of PPF and the efficacy and safety of nintedanib. The relative effect of nintedanib on reducing the rate of forced vital capacity decline was consistent across subgroups based on ILD diagnosis, HRCT pattern, and disease severity at baseline, and between patients who were and were not taking glucocorticoids or disease-modifying anti-rheumatic drugs and/or glucocorticoids at baseline. The adverse events most frequently associated with nintedanib were gastrointestinal, particularly diarrhea, but nintedanib was discontinued in only a minority of cases. The results of the INBUILD trial highlight the importance of prompt detection and treatment of PPF and the utility of nintedanib as a treatment option.
- Published
- 2024
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17. [Fibrophysematous lung changes and microbiota changes in senile persons.]
- Author
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Kuzminov OM, Musienko SK, Pushkin AS, Davydova EP, Evnevich YV, and Saginbaev UR
- Subjects
- Humans, Aged, 80 and over, Male, Female, Lung microbiology, Tomography, X-Ray Computed methods, Pulmonary Fibrosis physiopathology, Pulmonary Fibrosis microbiology, Pulmonary Fibrosis diagnosis, Pulmonary Fibrosis etiology, Aging physiology, Pulmonary Emphysema microbiology, Pulmonary Emphysema physiopathology, Pulmonary Emphysema diagnosis, Microbiota physiology
- Abstract
Research on the condition of the lungs in senile people is an urgent task. This is due to the fact that degenerative or age-associated changes in the respiratory system play an important role in the formation of senile asthenia syndrome and a decrease in the age-related viability of the body as a whole. CT-scans of patients aged 80-90 years were analyzed (n=31). Age-associated changes were evaluated: the presence of linear fibrosis, increased pulmonary pattern by the type of reticular (reticular) changes, the presence of gross fibrous reticular changes with cystic cavities and air bullae (by the type of «cellular lung»), as well as the presence of pulmonary emphysema. Most naturally, senile people show changes characteristic of linear pulmonary fibrosis and emphysema. The progression of the process leads to diffuse reticular changes in the interalveolar and intersegmental septa and, in adverse cases, to the formation of gross changes in the type of «cellular lung». Fibro-emphysematous changes are significantly more common in men. A microbiological study of the microbiota of the lower respiratory tract in elderly people was also carried out (n=16). When studying the microbiocenosis of the lower respiratory tract in elderly people, the following data were obtained: resident microflora was found in 71% and clinically significant microorganisms were found in 29%.
- Published
- 2024
18. Circadian regulation of pulmonary disease: the importance of timing.
- Author
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Cunningham PS, Jackson C, Chakraborty A, Cain J, Durrington HJ, and Blaikley JF
- Subjects
- Humans, Asthma physiopathology, Pulmonary Disease, Chronic Obstructive physiopathology, Pulmonary Fibrosis physiopathology, Respiratory Tract Infections physiopathology, Time Factors, Clinical Trials as Topic, Research Design, Circadian Clocks physiology, Lung Diseases physiopathology
- Abstract
Circadian regulation causes the activity of biological processes to vary over a 24-h cycle. The pathological effects of this variation are predominantly studied using two different approaches: pre-clinical models or observational clinical studies. Both these approaches have provided useful insights into how underlying circadian mechanisms operate and specifically which are regulated by the molecular oscillator, a key time-keeping mechanism in the body. This review compares and contrasts findings from these two approaches in the context of four common respiratory diseases (asthma, chronic obstructive pulmonary disease, pulmonary fibrosis, and respiratory infection). Potential methods used to identify and measure human circadian oscillations are also discussed as these will be useful outcome measures in future interventional human trials that target circadian mechanisms., (© 2023 The Author(s).)
- Published
- 2023
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19. TREM-1 exacerbates bleomycin-induced pulmonary fibrosis by aggravating alveolar epithelial cell senescence in mice.
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Xiong JB, Duan JX, Jiang N, Zhang CY, Zhong WJ, Yang JT, Liu YB, Su F, Zhou Y, Li D, Yang HH, and Guan CX
- Subjects
- Animals, Mice, Bleomycin toxicity, Hydrogen Peroxide metabolism, Myeloid Cells, Alveolar Epithelial Cells pathology, Pulmonary Fibrosis metabolism, Pulmonary Fibrosis physiopathology, Triggering Receptor Expressed on Myeloid Cells-1 metabolism
- Abstract
Our previous study showed that triggering receptors expressed on myeloid cell-1 (TREM-1) was upregulated in bleomycin (BLM)-induced pulmonary fibrosis (PF) mouse model. However, the role of TREM-1 in the development of PF and its underlying mechanism remain unclear. Herein, we report that the prophylactical blockade of TREM-1 using a decoy peptide dodecapeptide (LR12) exerted protective effects against BLM-induced PF in mice, with a higher survival rate, attenuated tissue injury, and less extracellular matrix deposition. Interestingly, therapeutic blockade of TREM-1 at the early stage of fibrosis also attenuated BLM-induced PF, suggesting a non-inflammatory effect. More importantly, we observed that TREM-1 blockade with LR12 significantly reduced the expression of the senescence-relative protein, including p16, p21, p53, and γ-H2AX in the lungs of PF mice. Notably, TREM-1 was upregulated in alveolar epithelial cells (AECs) and correlated with the levels of senescence markers in BLM-treated mice. In vitro, activating TREM-1 with an agonistic antibody exacerbated BLM-induced senescence in MLE12 cells, a murine AEC cell line. Furthermore, prophylactic or therapeutic blockade of TREM-1 protected MLE12 cells from senescence induced by BLM or H
2 O2 . In conclusion, our findings elucidate a pro-fibrotic effect of TREM-1 by inducing AECs senescence in PF, providing a potential strategy for fibrotic disease treatment., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)- Published
- 2022
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20. Inducible factors and interaction of pulmonary fibrosis induced by prenatal dexamethasone exposure in offspring rats.
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Zhang Z, Chen H, Yu P, Ge C, Fang M, Zhao X, Geng Q, and Wang H
- Subjects
- Animals, Disease Models, Animal, Female, Fetal Development genetics, Humans, Male, Pregnancy, Prenatal Exposure Delayed Effects genetics, Rats, Rats, Wistar, Sex Factors, Dexamethasone toxicity, Disease Susceptibility chemically induced, Fetal Development drug effects, Prenatal Exposure Delayed Effects physiopathology, Pulmonary Fibrosis chemically induced, Pulmonary Fibrosis physiopathology
- Abstract
This study aimed to investigate the correlation between prenatal dexamethasone exposure (PDE) and susceptibility to pulmonary fibrosis in offspring. Healthy female Wistar rats were given dexamethasone (0.2 mg/kg.d) or an equal volume of normal saline subcutaneously from 9 to 20 days after conception. Some of their female offspring underwent ovariectomy (OV) at 22 weeks after birth. All animals were euthanized at 28 weeks after birth. The morphological changes related to pulmonary fibrosis and extracellular matrix-related gene expression were detected, and Two-way ANOVA analyzed the interaction between PDE and OV. The results showed that adult offspring rats in FD group (female rats with PDE treatment) had early pulmonary fibrosis changes, such as pulmonary interstitial thickening, and increased expression of type IV collagen (COL4), α -smooth muscle actin (α-SMA) and fibronectin (FN) in lung tissues compared with those in FC group (female rats with saline treatment). In addition, adult offspring rats in FDO group (female rats with PDE and OV treatment) showed signs of pulmonary fibrosis, including apparent extracellular matrix deposition, increased lung injury scores (P<0.01, P<0.05), and extracellular matrix related gene expression (P<0.01, P<0.05), compared with rats in FDS (female rats with PDE treatment alone) or rats in FCO group (female rats with OV treatment alone). Moreover, PDE and OV had an interactive effect on the development of pulmonary fibrosis in female adult offspring. This study first reported the correlation between PDE and susceptibility to pulmonary fibrosis in female offspring rats, as well as the synergistic effect of PDE and OV in this pathological event, which provided a basis for further understanding of the pathogenesis of fetal originated pulmonary fibrosis., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
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21. Reciprocal regulation of IL-33 receptor-mediated inflammatory response and pulmonary fibrosis by TRAF6 and USP38.
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Yi XM, Li M, Chen YD, Shu HB, and Li S
- Subjects
- Autophagy, Down-Regulation, Humans, Inflammation metabolism, Interleukin-33 metabolism, Pulmonary Fibrosis chemically induced, Pulmonary Fibrosis metabolism, Signal Transduction, Ubiquitin-Specific Proteases genetics, Ubiquitination, Inflammation physiopathology, Interleukin-33 physiology, Intracellular Signaling Peptides and Proteins metabolism, Pulmonary Fibrosis physiopathology, Ubiquitin-Specific Proteases metabolism
- Abstract
The warning cytokine interleukin-33 receptor (IL-33R) mediates local inflammatory responses and plays crucial roles in the pathogenesis of immune diseases such as pulmonary fibrosis and rheumatoid arthritis. Whether and how IL-33R is regulated remain enigmatic. Here, we identified ubiquitin-specific protease 38 (USP38) as a negative regulator of IL-33R–mediated signaling. USP38 deficiency promotes interleukin-33 (IL-33)–induced downstream proinflammatory responses in vitro and in vivo. Usp38
−/− mice are more susceptible to inflammatory damage and death and developed more serious pulmonary fibrosis after bleomycin treatment. USP38 is constitutively associated with IL-33R and deconjugates its K27-linked polyubiquitination at K511, resulting in its autophagic degradation. We further show that the E3 ubiquitin ligase tumor necrosis factor receptor–associated factor 6 (TRAF6) catalyzes K27-linked polyubiquitination of IL-33R at K511, and that deficiency of TRAF6 inhibits IL-33–mediated signaling. Our findings suggest that K27-linked polyubiquitination and deubiquitination of IL-33R by TRAF6 and USP38 reciprocally regulate IL-33R level and signaling, which represents a critical mechanism in the regulation of IL-33–triggered lung inflammatory response and pulmonary fibrosis.- Published
- 2022
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22. Protective effect of dapsone against bleomycin-induced lung fibrosis in rat.
- Author
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Yousefi-Manesh H, Noori T, Asgardoon MH, Derakhshan MH, Tavangar SM, Sheibani M, Shirooie S, and Dehpour AR
- Subjects
- Animals, Anti-Inflammatory Agents administration & dosage, Anti-Inflammatory Agents pharmacology, Antioxidants administration & dosage, Antioxidants pharmacology, Bleomycin toxicity, Catalase metabolism, Dapsone administration & dosage, Disease Models, Animal, Glutathione Peroxidase metabolism, Histocytochemistry, Lung cytology, Lung pathology, Oxidative Stress drug effects, Peroxidase metabolism, Rats, Rats, Wistar, Superoxide Dismutase metabolism, Bleomycin adverse effects, Dapsone pharmacology, Pulmonary Fibrosis chemically induced, Pulmonary Fibrosis drug therapy, Pulmonary Fibrosis physiopathology
- Abstract
Idiopathic pulmonary fibrosis (IPF) is a chronic interstitial disease of the lung tissue that causes symptoms such as coughing and asthma. It is caused by inflammatory factors and oxidative stress. In vivo model of IPF is induced by bleomycin (BLM,) a chemotherapeutic agent. We have investigated the effect of dapsone on bleomycin-induced IPF in adult male Wistar rats due to its anti-inflammatory and anti-oxidative stress effects. The animals were randomly divided into 5 groups (Control, BLM, BLM + dapsone 1, BLM + Dapsone 3, BLM + Dapsone 10). The control group received normal water and food. In the fibrosis group, bleomycin (BLM) (5 mg/kg) was used to induce pulmonary fibrosis by intratracheal administration. Three groups of animals were treated daily with single doses of 1, 3, and 10 mg dapsone by intraperitoneal injection 1 h after receiving BLM for 2 weeks. The activity of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), and oxidative stress markers such as myeloperoxidase (MPO), malondialdehyde (MDA), protein carbonyl (PC) and nitrite were measured to evaluate bleomycin and therapeutic effect of dapsone. The histological assays of lung tissues were done by Hematoxylin-eosin (H & E) and Masson's trichrome staining. BLM reduced the activity of oxidative enzymes and increased the oxidative stress markers, while treatment with dapsone has reversed the results. In addition, the total number of cells as inflammatory cells such as neutrophils and eosinophils were examined. It has been indicated BLM increased these cells, and dapsone decreased them. The results of H & E and Masson's trichrome staining showed that dapsone reduced inflammation and alveolar wall thickness and BLM-induced pulmonary fibrosis. According to the findings of this study, dapsone seems to have therapeutic effects on pulmonary fibrosis through its anti-inflammatory and anti-oxidative stress properties and reduction of the toxic effects of bleomycin., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2022
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23. Histone methyltransferase SETDB1 inhibits TGF-β-induced epithelial-mesenchymal transition in pulmonary fibrosis by regulating SNAI1 expression and the ferroptosis signaling pathway.
- Author
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Liu T, Xu P, Ke S, Dong H, Zhan M, Hu Q, and Li J
- Subjects
- A549 Cells, Animals, Bleomycin, Epithelial-Mesenchymal Transition drug effects, Gene Knockdown Techniques, Histone-Lysine N-Methyltransferase genetics, Humans, Promoter Regions, Genetic physiology, Pulmonary Fibrosis chemically induced, Rats, Sprague-Dawley, Rats, Epithelial-Mesenchymal Transition physiology, Ferroptosis physiology, Histone-Lysine N-Methyltransferase metabolism, Pulmonary Fibrosis physiopathology, Snail Family Transcription Factors metabolism, Transforming Growth Factor beta pharmacology
- Abstract
The epithelial-mesenchymal transition (EMT) is an important pathological process in the occurrence of pulmonary fibrosis. Changes in histone methylation modifications of key genes play an important role in this process. As a histone methyltransferase, the regulatory mechanism and role of SET domain bifurcated 1 (SETDB1) in pulmonary fibrosis remain unclear. We found that SETDB1 inhibited EMT and that cells attenuated the expression of SETDB1 to relieve this inhibition during transforming growth factor-β (TGF-β)-induced EMT. Silencing SETDB1 expression significantly enhanced the mesenchymal phenotype induced by TGF-β and the expression and deposition of fibronectin and significantly reduced the expression of E-cadherin. The decrease in E-cadherin expression and the induction of EMT led to increased lipid reactive oxygen species (ROS) and ferrous ions, which induced ferroptosis. Chromatin immunoprecipitation (ChIP) results showed that SETDB1 regulates the expression of Snai1 by catalyzing the histone H3 lysine 9 trimethylation (H3K9me3) of Snai1, the main transcription factor that initiates the process of EMT, and thus, indirectly regulates E-cadherin. Surprisingly, when examining the effect of overexpressed SETDB1 on EMT, we found that overexpressed SETDB1 alleviated EMT and also caused ferroptosis. We suggest that the overexpression of SETDB1 partially reverses the mesenchymal phenotype to an epithelial state, while those cells that fail to reverse are depleted by ferroptosis. In conclusion, the histone methylase SETDB1 regulates Snai1 epigenetically, driving EMT gene reprogramming and ferroptosis in response to TGF-β. However, there are unexplored links between the epigenetic reprogramming and transcriptional processes that regulate EMT in a TGF-β-dependent manner., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2022
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24. Therapeutic potential of targeting cathepsin S in pulmonary fibrosis.
- Author
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Yoo Y, Choi E, Kim Y, Cha Y, Um E, Kim Y, Kim Y, and Lee YS
- Subjects
- Animals, Cathepsins metabolism, Drug Development, Extracellular Matrix metabolism, Humans, Hydrogen-Ion Concentration, Molecular Targeted Therapy, Pulmonary Fibrosis physiopathology, Cathepsins antagonists & inhibitors, Pulmonary Fibrosis drug therapy
- Abstract
Cathepsin S (CTSS), a lysosomal protease, belongs to a family of cysteine cathepsin proteases that promote degradation of damaged proteins in the endolysosomal pathway. Aberrant CTSS expression and regulation are associated with the pathogenesis of several diseases, including lung diseases. CTSS overexpression causes a variety of pathological processes, including pulmonary fibrosis, with increased CTSS secretion and accelerated extracellular matrix remodeling. Compared to many other cysteine cathepsin family members, CTSS has unique features that it presents limited tissue expression and retains its enzymatic activity at a neutral pH, suggesting its decisive involvement in disease microenvironments. In this review, we investigated the role of CTSS in lung disease, exploring recent studies that have indicated that CTSS mediates fibrosis in unique ways, along with its structure, substrates, and distinct regulation. We also outlined examples of CTSS inhibitors in clinical and preclinical development and proposed CTSS as a potential therapeutic target for pulmonary fibrosis., (Copyright © 2021 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Published
- 2022
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25. Iron Deficiency Is Associated With More Severe Pulmonary Vascular Disease in Pulmonary Hypertension Caused by Chronic Lung Disease.
- Author
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Tatah J, Keen JL, Prisco SZ, Pritzker M, Thenappan T, and Prins KW
- Subjects
- Aged, Chronic Disease, Female, Humans, Hypertension, Pulmonary etiology, Iron Deficiencies metabolism, Lung Diseases complications, Lung Diseases, Interstitial complications, Lung Diseases, Interstitial physiopathology, Male, Middle Aged, Pulmonary Disease, Chronic Obstructive complications, Pulmonary Disease, Chronic Obstructive physiopathology, Pulmonary Emphysema complications, Pulmonary Emphysema physiopathology, Pulmonary Fibrosis complications, Pulmonary Fibrosis physiopathology, Pulmonary Wedge Pressure physiology, Receptors, Transferrin metabolism, Severity of Illness Index, Sleep Apnea, Central complications, Sleep Apnea, Central physiopathology, Sleep Apnea, Obstructive complications, Sleep Apnea, Obstructive physiopathology, Vascular Resistance physiology, Vascular Stiffness physiology, Hypertension, Pulmonary epidemiology, Hypertension, Pulmonary physiopathology, Iron Deficiencies epidemiology, Lung Diseases physiopathology
- Published
- 2022
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26. Type 1 inflammatory endotype relates to low compliance, lung fibrosis, and severe complications in COVID-19.
- Author
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Hasegawa T, Nakagawa A, Suzuki K, Yamashita K, Yamashita S, Iwanaga N, Tamada E, Noda K, and Tomii K
- Subjects
- Aged, Biomarkers blood, COVID-19 blood, COVID-19 virology, Cluster Analysis, Disease Progression, Female, Humans, Inflammation blood, Inflammation complications, Lung Compliance, Male, Middle Aged, Pulmonary Fibrosis blood, Respiratory Distress Syndrome blood, Respiratory Distress Syndrome complications, SARS-CoV-2 physiology, COVID-19 complications, COVID-19 physiopathology, Inflammation pathology, Pulmonary Fibrosis complications, Pulmonary Fibrosis physiopathology
- Abstract
Background: Coronavirus disease 2019 (COVID-19) is an acute respiratory disease; approximately 5% of patients developing severe COVID-19. It is known that cytokine release is associated with disease severity, but the relationship between the different clinical phenotypes and inflammatory endotypes is not well understood., Objective: This study investigated the association between inflammatory biomarker-based endotypes and severe COVID-19 phenotypes., Methods: Interleukin (IL) -6, C-reactive protein (CRP), C-X-C motif chemokine (CXCL) 9, IL-18, C-C motif chemokine (CCL) 3, CCL17, IL-10, and vascular endothelial growth factor (VEGF) were measured in 57 COVID-19 patients, and their association with clinical characteristics was examined using a cluster analysis., Results: Significantly higher blood levels of the eight inflammatory markers were noted in patients who developed acute respiratory distress syndrome (ARDS) than in those who did not develop ARDS (non-ARDS). Using a cluster analysis, the patient groups were classified into four clusters, of which two had patients with high IL-6 and CRP levels. In the cluster with high levels of Type 1 (T1) inflammatory markers such as CXCL9 and IL-18, 85% of the patients had ARDS, 65% of the patients developed acute kidney injury (AKI), and 78% of the patients developed pulmonary fibrosis., Conclusions: In the cluster with high levels of T1 inflammatory markers, the patients frequently suffered from tissue damage, manifested as ARDS and AKI. Our findings identified distinct T1 inflammatory endotypes of COVID-19 and suggest the importance of controlling inflammation by monitoring T1 biomarkers and treating accordingly to limit the severity of the disease., (Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2021
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27. Renin angiotensin aldosterone system in pulmonary fibrosis: Pathogenesis to therapeutic possibilities.
- Author
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Gupta D, Kumar A, Mandloi A, and Shenoy V
- Subjects
- Angiotensin I metabolism, Angiotensin-Converting Enzyme 2 metabolism, Animals, Antifibrotic Agents therapeutic use, Humans, Lung drug effects, Lung pathology, Lung physiopathology, Molecular Targeted Therapy, Peptide Fragments metabolism, Proto-Oncogene Mas metabolism, Pulmonary Fibrosis drug therapy, Pulmonary Fibrosis pathology, Pulmonary Fibrosis physiopathology, Signal Transduction, Lung metabolism, Pulmonary Fibrosis metabolism, Renin-Angiotensin System drug effects
- Abstract
Pulmonary fibrosis is a devastating lung disease with multifactorial etiology characterized by alveolar injury, fibroblast proliferation and excessive deposition of extracellular matrix proteins, which progressively results in respiratory failure and death. Accumulating evidence from experimental and clinical studies supports a central role of the renin angiotensin aldosterone system (RAAS) in the pathogenesis and progression of idiopathic pulmonary fibrosis. Angiotensin II (Ang II), a key vasoactive peptide of the RAAS mediates pro-inflammatory and pro-fibrotic effects on the lungs, adversely affecting organ function. Recent years have witnessed seminal discoveries in the field of RAAS. Identification of new enzymes, peptides and receptors has led to the development of several novel concepts. Of particular interest is the establishment of a protective axis of the RAAS comprising of Angiotensin converting enzyme 2 (ACE2), Angiotensin-(1-7) [Ang-(1-7)], and the Mas receptor (the ACE2/Ang-(1-7)/Mas axis), and the discovery of a functional role for the Angiotensin type 2 (AT
2 ) receptor. Herein, we will review our current understanding of the role of RAAS in lung fibrogenesis, provide evidence on the anti-fibrotic actions of the newly recognized RAAS components (the ACE2/Ang-(1-7)/Mas axis and AT2 receptor), discuss potential strategies and translational efforts to convert this new knowledge into effective therapeutics for PF., (Copyright © 2021 Elsevier Ltd. All rights reserved.)- Published
- 2021
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28. Baseline Stiffness Modulates the Non-Linear Response to Stretch of the Extracellular Matrix in Pulmonary Fibrosis.
- Author
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Júnior C, Narciso M, Marhuenda E, Almendros I, Farré R, Navajas D, Otero J, and Gavara N
- Subjects
- Animals, Bleomycin, Disease Models, Animal, Elasticity, Male, Microscopy, Atomic Force, Rats, Sprague-Dawley, Rats, Extracellular Matrix physiology, Mechanotransduction, Cellular, Pulmonary Fibrosis physiopathology
- Abstract
Pulmonary fibrosis (PF) is a progressive disease that disrupts the mechanical homeostasis of the lung extracellular matrix (ECM). These effects are particularly relevant in the lung context, given the dynamic nature of cyclic stretch that the ECM is continuously subjected to during breathing. This work uses an in vivo model of pulmonary fibrosis to characterize the macro- and micromechanical properties of lung ECM subjected to stretch. To that aim, we have compared the micromechanical properties of fibrotic ECM in baseline and under stretch conditions, using a novel combination of Atomic Force Microscopy (AFM) and a stretchable membrane-based chip. At the macroscale, fibrotic ECM displayed strain-hardening, with a stiffness one order of magnitude higher than its healthy counterpart. Conversely, at the microscale, we found a switch in the stretch-induced mechanical behaviour of the lung ECM from strain-hardening at physiological ECM stiffnesses to strain-softening at fibrotic ECM stiffnesses. Similarly, we observed solidification of healthy ECM versus fluidization of fibrotic ECM in response to stretch. Our results suggest that the mechanical behaviour of fibrotic ECM under stretch involves a potential built-in mechanotransduction mechanism that may slow down the progression of PF by steering resident fibroblasts away from a pro-fibrotic profile.
- Published
- 2021
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29. Complications after discharge with COVID-19 infection and risk factors associated with development of post-COVID pulmonary fibrosis.
- Author
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Aul DR, Gates DJ, Draper DA, Dunleavy DA, Ruickbie DS, Meredith DH, Walters DN, van Zeller DC, Taylor DV, Bridgett DM, Dunwoody DR, Grubnic DS, Jacob DT, and Ean Ong DY
- Subjects
- Adult, COVID-19 epidemiology, Humans, Male, Middle Aged, Prevalence, Pulmonary Fibrosis diagnosis, Pulmonary Fibrosis physiopathology, Respiratory Function Tests, Risk Factors, United Kingdom epidemiology, COVID-19 complications, Lung physiopathology, Pandemics, Patient Discharge trends, Pulmonary Fibrosis etiology, SARS-CoV-2
- Abstract
Introduction: Survivors of COVID-19 infection may develop post-covid pulmonary fibrosis (PCF) and suffer from long term multi-system complications. The magnitude and risk factors associated with these are unknown., Objectives: We investigated the prevalence and risk factors associated with PCF and other complications in patients discharged after COVID-19 infection., Methods: Patients had phone assessment 6 weeks post hospital discharge after COVID-19 infection using a set protocol. Those with significant respiratory symptoms were investigated with a CTPA, Pulmonary Function Tests and echocardiogram. Prevalence of myalgia, fatigue, psychological symptoms and PCF was obtained. Risk factors associated with these were investigated., Results: A large number of patients had persistent fatigue (45.1%), breathlessness (36.5%), myalgia (20.5%) and psychological symptoms (19.5%). PCF was seen in 9.5% of the patients and was associated with persistent breathlessness at 6 weeks and inpatient ventilation [adjusted OR 5.02(1.76-14.27) and 4.45(1.27-15.58)] respectively. It was more common in men and in patients with peak CRP >171.5 mg/L, peak WBC count ≥12 × 10 9/L, severe inpatient COVID-19 CXR changes and CT changes. Ventilation was also a risk factor for persisting fatigue and myalgia, the latter was also more common in those with severe cytokine storm and severe COVID-19 inpatient CXR changes., Conclusions: All the patients discharged after COVID-19 should be assessed using a set protocol by a multidisciplinary team. Patients who had severe COVID-19 infection particularly those who were intubated and who have persistent breathlessness are at risk of developing PCF. They should have a CT Chest and have respiratory follow-up., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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30. Cardiac Fibrosis Is a Risk Factor for Severe COVID-19.
- Author
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Mustroph J, Hupf J, Baier MJ, Evert K, Brochhausen C, Broeker K, Meindl C, Seither B, Jungbauer C, Evert M, Maier LS, and Wagner S
- Subjects
- Adult, Aged, COVID-19 immunology, Female, Fibrosis, Heart Ventricles metabolism, Humans, Interleukin-1 Receptor-Like 1 Protein genetics, Interleukin-1 Receptor-Like 1 Protein metabolism, Male, Middle Aged, Myocardium metabolism, Neuropilin-1 genetics, Neuropilin-1 metabolism, Pulmonary Fibrosis immunology, Risk, Severity of Illness Index, Transforming Growth Factor beta1 genetics, Transforming Growth Factor beta1 metabolism, Viral Load, COVID-19 physiopathology, Heart Ventricles pathology, Myocardium pathology, Pulmonary Fibrosis physiopathology, SARS-CoV-2 physiology
- Abstract
Increased left ventricular fibrosis has been reported in patients hospitalized with coronavirus disease 2019 (COVID-19). It is unclear whether this fibrosis is a consequence of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infection or a risk factor for severe disease progression. We observed increased fibrosis in the left ventricular myocardium of deceased COVID-19 patients, compared with matched controls. We also detected increased mRNA levels of soluble interleukin-1 receptor-like 1 (sIL1-RL1) and transforming growth factor β1 (TGF-β1) in the left ventricular myocardium of deceased COVID-19 patients. Biochemical analysis of blood sampled from patients admitted to the emergency department (ED) with COVID-19 revealed highly elevated levels of TGF-β1 mRNA in these patients compared to controls. Left ventricular strain measured by echocardiography as a marker of pre-existing cardiac fibrosis correlated strongly with blood TGF-β1 mRNA levels and predicted disease severity in COVID-19 patients. In the left ventricular myocardium and lungs of COVID-19 patients, we found increased neuropilin-1 (NRP-1) RNA levels, which correlated strongly with the prevalence of pulmonary SARS-CoV-2 nucleocapsid. Cardiac and pulmonary fibrosis may therefore predispose these patients to increased cellular viral entry in the lung, which may explain the worse clinical outcome observed in our cohort. Our study demonstrates that patients at risk of clinical deterioration can be identified early by echocardiographic strain analysis and quantification of blood TGF-β1 mRNA performed at the time of first medical contact., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Mustroph, Hupf, Baier, Evert, Brochhausen, Broeker, Meindl, Seither, Jungbauer, Evert, Maier and Wagner.)
- Published
- 2021
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31. Experimental study on the effect of Si and P ion content in SiO2 exposure environment on the degree of pulmonary fibrosis.
- Author
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Hang W, Zhao J, Li Y, Wang L, and Li H
- Subjects
- Adult, Animals, Biomarkers analysis, Female, Humans, Male, Middle Aged, Occupational Diseases diagnosis, Occupational Diseases physiopathology, Rats, Rats, Sprague-Dawley, Silicosis physiopathology, Environmental Exposure adverse effects, Phosphorus analysis, Pulmonary Fibrosis chemically induced, Pulmonary Fibrosis physiopathology, Silicon analysis, Silicon Dioxide adverse effects, Silicosis diagnosis
- Abstract
Background: Silicosis is a public health issue in developing countries for long and cannot be completely cured., Objective: To study the changes of ion content with TNF-α and TGF-β expression in alveolar lavage fluid (BALF) at different time points in rats exposed to silica and to investigate their correlation with pulmonary fibrosis., Methods: 42 rats were randomly divided into control group (n = 12) and exposure group (n = 30). Tissues of right lower lungs were collected and fixed for further Hematoxylin-eosin (HE) and Masson staining. We collected the BALF to examine the inflammatory cytokines of TNF-α and TGF-β and measured the ion contents in BALF., Results: The increase of TNF-α level was earlier than TGF-β. The content of silica in BALF was significantly increased after exposure and reached the maximum at 7
th day, similar to the curve of cytokine TGF-β level. However, phosphorus ions increased quickly after gradual decline of silicon ion and roughly proportional to the curve of degree of fibrosis., Conclusions: Crystalline silica exposure can cause changes in TGF-β and TNF-α in BALF and accompanied with fibrosis and ions content variation. The abnormal expression of phosphorus ion may have significance in the occurrence and development of silicosis.- Published
- 2021
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32. GED-0507 attenuates lung fibrosis by counteracting myofibroblast transdifferentiation in vivo and in vitro.
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Speca S, Dubuquoy C, Rousseaux C, Chavatte P, Desreumaux P, and Spagnolo P
- Subjects
- A549 Cells, Animals, Bleomycin, Cell Line, Cell Survival drug effects, Humans, In Vitro Techniques, Inflammation, Lung drug effects, Mice, Mice, Inbred C57BL, PPAR gamma metabolism, Pulmonary Fibrosis physiopathology, Treatment Outcome, Cell Transdifferentiation, Myofibroblasts cytology, Propionates pharmacology, Pulmonary Fibrosis drug therapy
- Abstract
The development of more effective, better tolerated drug treatments for progressive pulmonary fibrosis (of which idiopathic pulmonary fibrosis is the most common and severe form) is a research priority. The peroxisome proliferator-activated receptor gamma (PPAR-γ) is a key regulator of inflammation and fibrosis and therefore represents a potential therapeutic target. However, the use of synthetic PPAR-γ agonists may be limited by their potentially severe adverse effects. In a mouse model of bleomycin (BLM)-induced pulmonary fibrosis, we have demonstrated that the non-racemic selective PPAR-γ modulator GED-0507 is able to reduce body weight loss, ameliorate clinical and histological features of pulmonary fibrosis, and increase survival rate without any safety concerns. Here, we focused on the biomolecular effects of GED-0507 on various inflammatory/fibrotic pathways. We demonstrated that preventive and therapeutic administration of GED-0507 reduced the BLM-induced mRNA expression of several markers of fibrosis, including transforming growth factor (TGF)-β, alpha-smooth muscle actin, collagen and fibronectin as well as epithelial-to-mesenchymal transition (EMT) and expression of mucin 5B. The beneficial effect of GED-0507 on pulmonary fibrosis was confirmed in vitro by its ability to control TGFβ-induced myofibroblast activation in the A549 human alveolar epithelial cell line, the MRC-5 lung fibroblast line, and primary human lung fibroblasts. Compared with the US Food and Drug Administration-approved antifibrotic drugs pirfenidone and nintedanib, GED-0507 displayed greater antifibrotic activity by controlling alveolar epithelial cell dysfunction, EMT, and extracellular matrix remodeling. In conclusion, GED-0507 demonstrated potent antifibrotic properties and might be a promising drug candidate for the treatment of pulmonary fibrosis., Competing Interests: PS serves as consultant for PPM Services S.A. Morbio Inferiore, a Swiss affiliate of Nogra Pharma Ltd http://www.nographarma.com/). Intestinal Biotech Development (IBD) provided support in the form of salaries for authors CD and CR. This does not alter our adherence to PLOS ONE policies on sharing data and materials. Concerning GED-0507 and IPF we declare the following patent and patent application numbers: U.S. Patent No. 8,153,841. U.S. Patent Application No. 20180065921.
- Published
- 2021
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33. miR-138 inhibits epithelial-mesenchymal transition in silica-induced pulmonary fibrosis by regulating ZEB2.
- Author
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Wu Q, Gui W, Jiao B, Han L, and Wang F
- Subjects
- A549 Cells, Animals, Cell Movement genetics, Disease Models, Animal, Environmental Pollutants administration & dosage, Environmental Pollutants toxicity, Epithelial Cells drug effects, Epithelial Cells metabolism, Fibrosis pathology, Gene Knockdown Techniques, Humans, Inhalation Exposure adverse effects, Male, Mice, Mice, Inbred C57BL, Pulmonary Fibrosis genetics, Pulmonary Fibrosis physiopathology, Silicon Dioxide administration & dosage, Silicosis physiopathology, Zinc Finger E-box Binding Homeobox 2 genetics, Zinc Finger E-box Binding Homeobox 2 metabolism, Epithelial-Mesenchymal Transition drug effects, MicroRNAs genetics, Pulmonary Fibrosis chemically induced, Silicon Dioxide toxicity
- Abstract
Silica dust is a common pollutant in the occupational environment, such as coal mines. Inhalation of silica dust can cause progressive pulmonary fibrosis and then silicosis. Silicosis is still one of the most harmful occupational diseases in the world, so the study of its pathogenesis is necessary for the treatment of silicosis. In this study, we constructed a mouse model of pulmonary fibrosis via intratracheal instillation of silica particles and identified the decreased expression of miR-138 in fibrotic lung tissues of mice. Moreover, the overexpression of miR-138 retarded the process of epithelial-mesenchymal transition (EMT) in a mouse model of silica particles exposure and epithelial cells stimulated by silica particles. Further studies showed that ZEB2 was one of the potential targets of miR-138, and the up-regulation of miR-138 reduced ZEB2 levels in mouse lung tissues and in epithelial cells. We next found that the expression levels of ɑ-SMA and Vimentin were significantly increased and E-cadherin levels were decreased after transfection with miR-138 inhibitor in epithelial cells. However, these effects were abated by the knockdown of ZEB2. Consistently, the increased migration ability of epithelial cells by miR-138 inhibitor transfection was also reversed by the knockdown of ZEB2. Collectively, we revealed that miR-138 significantly targeted ZEB2, thus inhibited the EMT process and mitigated the development of pulmonary fibrosis. miR-138 may be a potential target for the treatment of pulmonary fibrosis., (Copyright © 2021 Elsevier B.V. All rights reserved.)
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- 2021
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34. Evaluation on epithelial-mesenchymal state and microRNAs focusing on isolated alveolar epithelial cells from bleomycin injured rat lung.
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Kawami M, Takenaka S, Kadekaru Y, Akai M, Konaka T, Yumoto R, and Takano M
- Subjects
- Alveolar Epithelial Cells cytology, Alveolar Epithelial Cells drug effects, Animals, Antibiotics, Antineoplastic administration & dosage, Antibiotics, Antineoplastic toxicity, Bleomycin administration & dosage, Lung Injury genetics, Lung Injury physiopathology, Male, MicroRNAs genetics, Pulmonary Fibrosis genetics, Pulmonary Fibrosis physiopathology, Rats, Rats, Sprague-Dawley, Time Factors, Bleomycin toxicity, Epithelial-Mesenchymal Transition drug effects, Lung Injury chemically induced, Pulmonary Fibrosis chemically induced
- Abstract
Several studies using bleomycin (BLM)-induced lung injury rat model revealed that epithelial-mesenchymal transition (EMT) contributes to pulmonary fibrosis. Conversely, microRNAs (miRNAs) are considered as useful markers of various diseases. In the present study, we aimed to characterize the EMT state through focusing on alveolar epithelial cells and identify the miRNAs that can be used as markers to predict pulmonary fibrosis using a BLM-induced lung injury rat model. Intratracheal administration of BLM increased hydroxyproline, a component of collagen, in lung tissues at day 14, but not at day 7. However, BLM induced EMT at day 7, which was accompanied with increased mRNA expression of α-smooth muscle actin, a representative EMT marker, in alveolar epithelium, thereby suggesting that EMT occurs prior to pulmonary fibrosis in alveolar epithelial cells. Using this rat model, the expression levels of several EMT-associated miRNAs were examined, and miR-222 was found to be upregulated in alveolar epithelial cells as well as bronchoalveolar lavage fluid from day 3. Our findings indicate that EMT in alveolar epithelial cells may occur before pulmonary fibrosis, and miR-222 may be used as a potential marker for early prediction of pulmonary fibrosis., (Copyright © 2021 Elsevier B.V. All rights reserved.)
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- 2021
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35. Pulmonary function changes in older adults with and without metabolic syndrome.
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Brandao-Rangel MAR, Moraes-Ferreira R, Oliveira-Junior MC, Santos-Dias A, Bachi ALL, Gabriela-Pereira G, de Oliveira Freitas S, Araújo-Rosa AC, Oliveira LVF, Frison CR, do Prado WL, Raju RP, Balagopal PB, and Vieira RP
- Subjects
- Aged, Anthropometry, Biomarkers metabolism, Cross-Sectional Studies, Cytokines metabolism, Female, Hand Strength, Humans, Inflammation, Male, Middle Aged, Muscle Strength, Oscillometry, Pulmonary Fibrosis physiopathology, Respiratory Physiological Phenomena, Lung physiopathology, Metabolic Syndrome physiopathology, Respiratory Function Tests
- Abstract
The low-grade inflammation associated with metabolic syndrome (MS) triggers functional and structural alterations in several organs. Whereas lung function impairment is well reported for older adult population, the effect of MS on functional and immunological responses in the lungs remains unclear. In this cross-sectional study we determined whether MS alters pulmonary function, and immunological responses in older adults with MS. The study sample consisted of older adults with MS (68 ± 3 years old; n = 77) and without MS (67 ± 3 years old; n = 77). Impulse oscillometry was used to evaluate airway and tissue resistance, and reactance. Biomarkers of inflammation and fibrosis were assessed in the blood and in breath condensate. The total resistance of the respiratory system (R5Hz; p < 0.009), and the resistance of the proximal (R20Hz; p < 0.001) and distal (R5Hz-R20Hz; p < 0.004) airways were higher in MS individuals compared to those without MS. Pro-inflammatory (leptin, IL-1beta, IL-8, p < 0.001; TNF-alpha, p < 0.04) and anti-inflammatory cytokines (adiponectin, IL-1ra, IL-10, p < 0.001), anti-fibrotic (relaxin 1, relaxin 3, Klotho, p < 0.001) and pro-fibrotic (VEGF, p < 0.001) factors were increased in sera and in breath condensate individuals with MS. The results show that MS adversely affect lung mechanics, function, and immunological response in older adults. The data offer a metabolic basis for the inflammaging of the lungs and suggest the lungs as a potential therapeutic target for controlling the immune response and delaying the onset of impaired lung function in older adults with MS., (© 2021. The Author(s).)
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- 2021
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36. Pleuroparenchymal fibroelastosis in a patient with systemic sclerosis.
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Ufuk F, Altinisik G, and Karasu U
- Subjects
- Aged, Bronchiectasis diagnostic imaging, Disease Progression, Elastic Tissue pathology, Female, Humans, Lung Diseases, Interstitial pathology, Lung Diseases, Interstitial physiopathology, Pleural Diseases pathology, Pleural Diseases physiopathology, Pulmonary Diffusing Capacity, Pulmonary Fibrosis drug therapy, Pulmonary Fibrosis pathology, Pulmonary Fibrosis physiopathology, Pyridones therapeutic use, Scleroderma, Systemic pathology, Scleroderma, Systemic physiopathology, Tomography, X-Ray Computed, Vital Capacity, Elastic Tissue diagnostic imaging, Lung Diseases, Interstitial diagnostic imaging, Pleural Diseases diagnostic imaging, Pulmonary Fibrosis diagnostic imaging, Scleroderma, Systemic diagnostic imaging
- Published
- 2021
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37. Mechanisms of Endothelial-to-Mesenchymal Transition Induction by Extracellular Matrix Components in Pulmonary Fibrosis.
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Grigorieva OA, Vigovskiy MA, Dyachkova UD, Basalova NA, Aleksandrushkina NA, Kulebyakina MA, Zaitsev IL, Popov VS, and Efimenko AY
- Subjects
- Animals, Cell Differentiation drug effects, Cells, Cultured, Decellularized Extracellular Matrix chemistry, Decellularized Extracellular Matrix metabolism, Decellularized Extracellular Matrix pharmacology, Endothelial Cells drug effects, Endothelial Cells physiology, Epithelial-Mesenchymal Transition drug effects, Extracellular Matrix metabolism, Female, Fibroblasts drug effects, Fibroblasts pathology, Fibroblasts physiology, Human Umbilical Vein Endothelial Cells, Humans, Lung drug effects, Lung metabolism, Lung pathology, Male, Mice, Mice, Inbred C57BL, Myofibroblasts drug effects, Myofibroblasts physiology, Pulmonary Fibrosis metabolism, Pulmonary Fibrosis physiopathology, Signal Transduction drug effects, Signal Transduction physiology, Tissue Scaffolds, Epithelial-Mesenchymal Transition physiology, Extracellular Matrix physiology, Pulmonary Fibrosis pathology
- Abstract
Idiopathic pulmonary fibrosis can be caused by different factors, including accumulation of pathological extracellular matrix (ECM) with abnormal composition, stiffness, and architecture in the lung tissue. We studied the effect of ECM produced by lung fibroblasts of healthy mice or mice with bleomycin-induced pulmonary fibrosis on the process of endothelialto- mesenchymal transition, one of the main sources of effector myofibroblasts in fibrosis progression. Despite stimulation of spontaneous and TGFβ-1-induced differentiation of fibroblasts into myofibroblasts by fibrotic ECM, the appearance of α-SMA, the main marker of myofibroblasts, and its integration in stress fibrils in endotheliocytes were not observed under similar conditions. However, the expression of transcription factors SNAI1 and SNAI2/Slug and the production of components of fibrotic ECM (specific EDA-fibronectin splice form and collagen type I) were increased in endotheliocytes cultured on fibrotic ECM. Endothelium also demonstrated increased cell velocity in the models of directed cell migration. These data indicate activation of the intermediate state of the endothelial-to-mesenchymal transition in endotheliocytes upon contact with fibrotic, but not normal stromal matrix. In combination with the complex microenvironment that develops during fibrosis progression, it can lead to the replenishment of myofibroblasts pool from the resident endothelium., (© 2021. Springer Science+Business Media, LLC, part of Springer Nature.)
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- 2021
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38. Pulmonary fibrosis and its related factors in discharged patients with new corona virus pneumonia: a cohort study.
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Li X, Shen C, Wang L, Majumder S, Zhang D, Deen MJ, Li Y, Qing L, Zhang Y, Chen C, Zou R, Lan J, Huang L, Peng C, Zeng L, Liang Y, Cao M, Yang Y, Yang M, Tan G, Tang S, Liu L, Yuan J, and Liu Y
- Subjects
- Adolescent, Adult, COVID-19 complications, COVID-19 diagnosis, China, Female, Host-Pathogen Interactions, Humans, Lung diagnostic imaging, Lung physiopathology, Male, Middle Aged, Prognosis, Pulmonary Fibrosis diagnostic imaging, Pulmonary Fibrosis physiopathology, Respiratory Function Tests, Time Factors, Tomography, X-Ray Computed, Young Adult, COVID-19 virology, Lung virology, Patient Discharge, Pulmonary Fibrosis virology, SARS-CoV-2 pathogenicity
- Abstract
Background: Thousands of Coronavirus Disease 2019 (COVID-19) patients have been discharged from hospitals Persistent follow-up studies are required to evaluate the prevalence of post-COVID-19 fibrosis., Methods: This study involves 462 laboratory-confirmed patients with COVID-19 who were admitted to Shenzhen Third People's Hospital from January 11, 2020 to April 26, 2020. A total of 457 patients underwent thin-section chest CT scans during the hospitalization or after discharge to identify the pulmonary lesion. A total of 287 patients were followed up from 90 to 150 days after the onset of the disease, and lung function tests were conducted about three months after the onset. The risk factors affecting the persistence of pulmonary fibrosis were identified through regression analysis and the prediction model of the persistence of pulmonary fibrosis was established., Results: Parenchymal bands, irregular interfaces, reticulation and traction bronchiectasis were the most common CT features in all COVID-19 patients. During the 0-30, 31-60, 61-90, 91-120 and > 120 days after onset, 86.87%, 74.40%, 79.56%, 68.12% and 62.03% patients developed with pulmonary fibrosis and 4.53%, 19.61%, 18.02%, 38.30% and 48.98% patients reversed pulmonary fibrosis, respectively. It was observed that Age, BMI, Fever, and Highest PCT were predictive factors for sustaining fibrosis even after 90 days from onset. A predictive model of the persistence with pulmonary fibrosis was developed based-on the Logistic Regression method with an accuracy, PPV, NPV, Sensitivity and Specificity of the model of 76%, 71%, 79%, 67%, and 82%, respectively. More than half of the COVID-19 patients revealed abnormal conditions in lung function after 90 days from onset, and the ratio of abnormal lung function did not differ on a statistically significant level between the fibrotic and non-fibrotic groups., Conclusions: Persistent pulmonary fibrosis was more likely to develop in patients with older age, higher BMI, severe/critical condition, fever, a longer viral clearance time, pre-existing disease and delayed hospitalization. Fibrosis developed in COVID-19 patients could be reversed in about a third of the patients after 120 days from onset. The pulmonary function of less than half of COVID-19 patients could turn to normal condition after three months from onset. An effective prediction model with an average area under the curve (AUC) of 0.84 was established to predict the persistence of pulmonary fibrosis in COVID-19 patients for early diagnosis., (© 2021. The Author(s).)
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- 2021
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39. Validation and minimum important difference of the UCSD Shortness of Breath Questionnaire in fibrotic interstitial lung disease.
- Author
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Chen T, Tsai APY, Hur SA, Wong AW, Sadatsafavi M, Fisher JH, Johannson KA, Assayag D, Morisset J, Shapera S, Khalil N, Fell CD, Manganas H, Cox G, To T, Gershon AS, Hambly N, Halayko AJ, Wilcox PG, Kolb M, and Ryerson CJ
- Subjects
- Aged, Canada epidemiology, Cohort Studies, Dyspnea physiopathology, Female, Humans, Lung Diseases, Interstitial physiopathology, Male, Middle Aged, Prospective Studies, Pulmonary Fibrosis diagnosis, Pulmonary Fibrosis epidemiology, Pulmonary Fibrosis physiopathology, Registries standards, Reproducibility of Results, Vital Capacity physiology, Dyspnea diagnosis, Dyspnea epidemiology, Lung Diseases, Interstitial diagnosis, Lung Diseases, Interstitial epidemiology, Surveys and Questionnaires standards
- Abstract
Rationale: The University of California, San Diego Shortness of Breath Questionnaire (UCSDSOBQ) is a frequently used domain-specific dyspnea questionnaire; however, there is little information available regarding its use and minimum important difference (MID) in fibrotic interstitial lung disease (ILD). We aimed to describe the key performance characteristics of the UCSDSOBQ in this population., Methods: UCSDSOBQ scores and selected anchors were measured in 1933 patients from the prospective multi-center Canadian Registry for Pulmonary Fibrosis. Anchors included the St. George's Respiratory Questionnaire (SGRQ), European Quality of Life 5 Dimensions 5 Levels questionnaire (EQ-5D-5L) and EQ visual analogue scale (EQ-VAS), percent-predicted forced vital capacity (FVC%), diffusing capacity of the lung for carbon monoxide (DLCO%), and 6-min walk distance (6MWD). Concurrent validity, internal consistency, ceiling and floor effects, and responsiveness were assessed, followed by estimation of the MID by anchor-based (linear regression) and distribution-based methods (standard error of measurement)., Results: The UCSDSOBQ had a high level of internal consistency (Cronbach's alpha = 0.97), no obvious floor or ceiling effect, strong correlations with SGRQ, EQ-5D-5L, and EQ-VAS (|r| > 0.5), and moderate correlations with FVC%, DLCO%, and 6MWD (0.3 < |r| < 0.5). The MID estimate for UCSDSOBQ was 5 points (1-8) for the anchor-based method, and 4.5 points for the distribution-based method., Conclusion: This study demonstrates the validity of UCSDSOBQ in a large and heterogeneous population of patients with fibrotic ILD, and provides a robust MID estimate of 5-8 points.
- Published
- 2021
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40. Airway compliance measurements in mouse models of respiratory diseases.
- Author
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Robichaud A, Fereydoonzad L, Collins SL, Loube JM, Ishii Y, Horton MR, Martin JG, and Mitzner W
- Subjects
- Animals, Antibiotics, Antineoplastic toxicity, Female, Lung Compliance, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Pulmonary Emphysema etiology, Pulmonary Fibrosis chemically induced, Respiratory Mechanics, Airway Resistance, Bleomycin toxicity, Pulmonary Emphysema pathology, Pulmonary Fibrosis physiopathology, Respiration Disorders complications
- Abstract
The quantification of airway compliance ( C
aw ) is essential to the study of airway alterations in disease models. However, the required measurements of airway pressure and volume are difficult to acquire in mice. We hypothesized that the inflation limb of full-range pressure-volume (PV) curves could be used to quantify Caw , as it contains a segment where only the airway tree is distended. The study objective was to assess the feasibility of the approach by analysis of full-range PV curves previously collected in three mouse models: an elastase model of emphysema, a genetic model spontaneously developing emphysema (leukotriene C4 synthase knockout; LTC4S-KO), and a bleomycin model of lung fibrosis. Attempts to validate results included Caw change relative to respiratory system compliance (Δ Caw /Δ C ), the minute work of breathing (mWOB), and the elastance at 20.5 Hz ( Ers _20.5) from prior respiratory mechanics measurements in the same subjects. Caw was estimated at 3% of total compliance in healthy mice or 2.3 ± 1 μL/cmH2 O ( n = 17). The technique detected changes in models of respiratory obstructive and restrictive diseases relative to control mice as well as differences in the two emphysema models studied. The changes in Caw were consistent with those seen in Δ Caw /Δ C , mWOB, or Ers _20.5, with some variations according to the model, as well as with results reported in the literature in humans and mice. Direct Caw measurements in subjects as small as mice could prove useful to further characterize other respiratory disease models associated with airway remodeling or to assess treatment effects.- Published
- 2021
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41. Short-Term Consequences of SARS-CoV-2-Related Pneumonia: A Follow Up Study.
- Author
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Boari GEM, Bonetti S, Braglia-Orlandini F, Chiarini G, Faustini C, Bianco G, Santagiuliana M, Guarinoni V, Saottini M, Viola S, Ferrari-Toninelli G, Pasini G, Bonzi B, Desenzani P, Tusi C, Malerba P, Zanotti E, Turini D, and Rizzoni D
- Subjects
- COVID-19 complications, COVID-19 diagnosis, Follow-Up Studies, Host-Pathogen Interactions, Humans, Italy, Lung pathology, Lung physiopathology, Lung Diseases, Interstitial diagnosis, Lung Diseases, Interstitial physiopathology, Prognosis, Prospective Studies, Pulmonary Diffusing Capacity, Pulmonary Fibrosis diagnosis, Pulmonary Fibrosis physiopathology, Quality of Life, Time Factors, COVID-19 virology, Lung virology, Lung Diseases, Interstitial virology, Pulmonary Fibrosis virology, SARS-CoV-2 pathogenicity
- Abstract
The aim of the study was to assess the short-term consequences of SARS-CoV-2-related pneumonia, also in relation to radiologic/laboratory/clinical indices of risk at baseline. This prospective follow-up cohort study included 94 patients with confirmed COVID-19 admitted to a medical ward at the Montichiari Hospital, Brescia, Italy from February 28th to April 30th, 2020. Patients had COVID-19 related pneumonia with respiratory failure. Ninety-four patients out of 193 survivors accepted to be re-evaluated after discharge, on average after 4 months. In ¼ of the patients an evidence of pulmonary fibrosis was detected, as indicated by an altered diffusing capacity of the lung for carbon monoxide (DLCO); in 6-7% of patients the alteration was classified as of moderate/severe degree. We also evaluated quality of life thorough a structured questionnaire: 52% of the patients still lamented fatigue, 36% effort dyspnea, 10% anorexia, 14% dysgeusia or anosmia, 31% insomnia and 21% anxiety. Finally, we evaluated three prognostic indices (the Brixia radiologic score, the Charlson Comorbidity Index and the 4C mortality score) in terms of prediction of the clinical consequences of the disease. All of them significantly predicted the extent of short-term lung involvement. In conclusion, our study demonstrated that SARS-CoV-2-related pneumonia is associated to relevant short-term clinical consequences, both in terms of persistence of symptoms and in terms of impairment of DLCO (indicator of a possible development of pulmonary fibrosis); some severity indices of the disease may predict short-term clinical outcome. Further studies are needed to ascertain whether such manifestations may persist long-term.
- Published
- 2021
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42. Oxygen supplementation during exercise improves leg muscle fatigue in chronic fibrotic interstitial lung disease.
- Author
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Marillier M, Bernard AC, Verges S, Moran-Mendoza O, O'Donnell DE, and Neder JA
- Subjects
- Female, Humans, Male, Pulmonary Fibrosis physiopathology, Exercise Tolerance physiology, Muscle Fatigue physiology, Oxygen Consumption physiology, Oxygen Inhalation Therapy methods, Pulmonary Fibrosis rehabilitation, Respiratory Muscles physiopathology
- Abstract
Background: Exercise-induced hypoxaemia is a hallmark of chronic fibrotic interstitial lung disease ( f -ILD). It remains unclear whether patients' severe hypoxaemia may exaggerate locomotor muscle fatigue and, if so, to what extent oxygen (O
2 ) supplementation can ameliorate these abnormalities., Methods: Fifteen patients (12 males, 9 with idiopathic pulmonary fibrosis) performed a constant-load (60% peak work rate) cycle test to symptom limitation (Tlim) while breathing medical air. Fifteen age-matched and sex-matched controls cycled up to patients' Tlim. Patients repeated the exercise test on supplemental O2 (42%±7%) for the same duration. Near-infrared spectroscopy assessed vastus lateralis oxyhaemoglobin concentration ((HbO2 )). Pre-exercise to postexercise variation in twitch force (∆Tw) induced by femoral nerve magnetic stimulation quantified muscle fatigue., Results: Patients showed severe hypoxaemia (lowest O2 saturation by pulse oximetry=80.0%±7.6%) which was associated with a blunted increase in muscle (HbO2) during exercise vs controls (+1.3±0.3 µmol vs +4.4±0.4 µmol, respectively; p<0.001). Despite exercising at work rates ∼ one-third lower than controls (42±13 W vs 66±13 W), ∆Tw was greater in patients (∆Tw/external work performed by the leg muscles=-0.59±0.21 %/kJ vs -0.25±0.19 %/kJ; p<0.001). Reversal of exertional hypoxaemia with supplemental O2 was associated with a significant increase in muscle (HbO2) , leading to a reduced decrease in ∆Tw in patients (-0.33±0.19 %/kJ; p<0.001 vs air). Supplemental O2 significantly improved leg discomfort (p=0.005)., Conclusion: O2 supplementation during exercise improves leg muscle oxygenation and fatigue in f -ILD. Lessening peripheral muscle fatigue to enhance exercise tolerance is a neglected therapeutic target that deserves clinical attention in this patient population., Competing Interests: Competing interests: MM was funded by a European Respiratory Society’s Long Term Fellowship and the John Alexander Stuart Fellowship, Queen’s University. JAN has been funded by the New Clinician Scientist Program from the Southeastern Ontario Academic Medical Association (SEAMO), Canada. His laboratory was established owing to the Canadian Foundation for Innovation’s John R. Evans Leaders Fund, Canada., (© Author(s) (or their employer(s)) 2021. No commercial re-use. See rights and permissions. Published by BMJ.)- Published
- 2021
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43. Pulmonary Apical Cap as a Potential Risk Factor for Pleuroparenchymal Fibroelastosis.
- Author
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Marinescu DC, English J, Sedlic T, Kliber A, Ryerson CJ, and Wong AW
- Subjects
- Aged, Biopsy methods, Cachexia diagnosis, Cachexia etiology, Coronary Artery Bypass adverse effects, Diagnosis, Differential, Disease Progression, Dyspnea diagnosis, Dyspnea etiology, Fatal Outcome, Humans, Long Term Adverse Effects pathology, Long Term Adverse Effects physiopathology, Male, Respiratory Function Tests methods, Tomography, X-Ray Computed methods, Intraoperative Complications pathology, Intraoperative Complications physiopathology, Lung diagnostic imaging, Lung pathology, Lung Diseases, Interstitial etiology, Lung Diseases, Interstitial pathology, Lung Diseases, Interstitial physiopathology, Lung Injury complications, Lung Injury pathology, Lung Injury physiopathology, Pulmonary Fibrosis etiology, Pulmonary Fibrosis pathology, Pulmonary Fibrosis physiopathology
- Abstract
Pleuroparenchymal fibroelastosis (PPFE) is a progressive and frequently fatal interstitial lung disease that involves the upper lobes. Although its cause remains unknown, the histopathologic evidence underlying PPFE bears striking resemblance to that of the pulmonary apical cap (PAC), a relatively common and benign entity. We describe the case of a patient with PAC that evolved into distinctly asymmetric PPFE over 6 years after unilateral surgical lung injury. Given the histologic similarity between these two conditions, we propose that these two entities underlie common biologic pathways of abnormal response to lung injury, with the presence of a PAC increasing susceptibility to the development of PPFE in the face of ongoing inflammatory insults. This case describes the histopathologic evolution of PAC to PPFE before and after an inciting injury., (Copyright © 2021 American College of Chest Physicians. Published by Elsevier Inc. All rights reserved.)
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- 2021
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44. Protective Effects of Thymoquinone, an Active Compound of Nigella sativa , on Rats with Benzo(a)pyrene -Induced Lung Injury through Regulation of Oxidative Stress and Inflammation.
- Author
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Alzohairy MA, Khan AA, Alsahli MA, Almatroodi SA, and Rahmani AH
- Subjects
- Animals, Antioxidants chemistry, Cholesterol chemistry, DNA Fragmentation, Intercellular Adhesion Molecule-1 biosynthesis, Interleukin-1beta biosynthesis, Interleukin-6 biosynthesis, Lung pathology, Male, Pulmonary Fibrosis physiopathology, Rats, Treatment Outcome, Tumor Necrosis Factor-alpha biosynthesis, Benzo(a)pyrene chemistry, Benzoquinones analysis, Benzoquinones pharmacology, Inflammation, Lipids chemistry, Lung drug effects, Lung Injury chemically induced, Nigella sativa metabolism, Nitric Oxide chemistry, Oxidative Stress, Pulmonary Fibrosis chemically induced
- Abstract
Benzopyrene [B(a)P] is a well-recognized environmental carcinogen, which promotes oxidative stress, inflammation, and other metabolic complications. In the current study, the therapeutic effects of thymoquinone (TQ) against B(a)P-induced lung injury in experimental rats were examined. B(a)P used at 50 mg/kg b.w. induced lung injury that was investigated via the evaluation of lipid profile, inflammatory markers, nitric oxide (NO), and malondialdehyde (MDA) levels. B(a)P also led to a decrease in superoxide dismutase (SOD) (34.3 vs. 58.5 U/mg protein), glutathione peroxidase (GPx) (42.4 vs. 72.8 U/mg protein), catalase (CAT) (21.2 vs. 30.5 U/mg protein), and total antioxidant capacity compared to normal animals. Treatment with TQ, used at 50 mg/kg b.w., led to a significant reduction in triglycerides (TG) (196.2 vs. 233.7 mg/dL), total cholesterol (TC) (107.2 vs. 129.3 mg/dL), and inflammatory markers and increased the antioxidant enzyme level in comparison with the group that was administered B(a)P only ( p < 0.05). B(a)P administration led to the thickening of lung epithelium, increased inflammatory cell infiltration, damaged lung tissue architecture, and led to accumulation of collagen fibres as studied through haematoxylin and eosin (H&E), Sirius red, and Masson's trichrome staining. Moreover, the recognition of apoptotic nuclei and expression pattern of NF-κB were evaluated through the TUNEL assay and immunohistochemistry, respectively. The histopathological changes were found to be considerably low in the TQ-treated animal group. The TUNEL-positive cells increased significantly in the B(a)P-induced group, whereas the TQ-treated group showed a decreased apoptosis rate. Significantly high cytoplasmic expression of NF-κB in the B(a)P-induced group was seen, and this expression was prominently reduced in the TQ-treated group. Our results suggest that TQ can be used in the protection against benzopyrene-caused lung injury.
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- 2021
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45. Acute exacerbation of fibrotic hypersensitivity pneumonitis: incidence and outcomes.
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Kang J, Kim YJ, Choe J, Chae EJ, and Song JW
- Subjects
- Aged, Alveolitis, Extrinsic Allergic diagnosis, Alveolitis, Extrinsic Allergic mortality, Alveolitis, Extrinsic Allergic physiopathology, Disease Progression, Dyspnea diagnosis, Dyspnea mortality, Dyspnea physiopathology, Female, Hospital Mortality, Humans, Incidence, Lung pathology, Lung physiopathology, Male, Middle Aged, Prognosis, Pulmonary Fibrosis diagnosis, Pulmonary Fibrosis mortality, Pulmonary Fibrosis physiopathology, Retrospective Studies, Risk Assessment, Risk Factors, Seoul epidemiology, Time Factors, Alveolitis, Extrinsic Allergic epidemiology, Dyspnea epidemiology, Pulmonary Fibrosis epidemiology
- Abstract
Background: Patients with fibrotic hypersensitivity pneumonitis (HP) show variable clinical courses, and some experience rapid deterioration (RD), including acute exacerbation (AE). However, little is known about AE in fibrotic HP. Here, we retrospectively examined the incidence, risk factors, and outcomes of AE in fibrotic HP., Methods: The incidence rates of AE were calculated in 101 patients with biopsy-proven HP. AE was defined as the worsening of dyspnoea within 30 days, with new bilateral lung infiltration and no evidence of infection or other causes of dyspnoea., Results: During follow-up (median: 30 months), 18 (17.8%) patients experienced AE. The 1, 3, and 5 year incidence rates of AE were 6.0, 13.6, and 22.8%, respectively. Lower diffusing capacity of the lung for carbon monoxide (DL
CO ) and a radiologic usual interstitial pneumonia (UIP)-like pattern were risk factors for AE. In-hospital mortality after AE was 44.4%. Median survival from diagnosis was significantly shorter in patients with AE (26.0 months) than in those with no-AE RD (55.0 months; p = 0.008) or no RD (not reached; p < 0.001). AE remained a significant predictor of all-cause mortality (hazard ratio, 8.641; 95% confidence interval, 3.388-22.040; p < 0.001) after adjustment for age, body mass index, lung function, lymphocyte levels in bronchoalveolar lavage fluid, and the presence of a UIP-like pattern., Conclusions: AE was not uncommon among patients with fibrotic HP and significantly affected prognosis. A lower DLCO value and radiologic UIP-like pattern at diagnosis were associated with the development AE in patients with fibrotic HP.- Published
- 2021
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46. Pirfenidone in patients with progressive fibrotic interstitial lung diseases other than idiopathic pulmonary fibrosis (RELIEF): a double-blind, randomised, placebo-controlled, phase 2b trial.
- Author
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Behr J, Prasse A, Kreuter M, Johow J, Rabe KF, Bonella F, Bonnet R, Grohe C, Held M, Wilkens H, Hammerl P, Koschel D, Blaas S, Wirtz H, Ficker JH, Neumeister W, Schönfeld N, Claussen M, Kneidinger N, Frankenberger M, Hummler S, Kahn N, Tello S, Freise J, Welte T, Neuser P, and Günther A
- Subjects
- Anti-Inflammatory Agents, Non-Steroidal pharmacology, Dose-Response Relationship, Drug, Double-Blind Method, Drug Monitoring methods, Early Termination of Clinical Trials, Female, Humans, Intention to Treat Analysis, Male, Middle Aged, Symptom Assessment statistics & numerical data, Lung Diseases, Interstitial diagnosis, Lung Diseases, Interstitial drug therapy, Lung Diseases, Interstitial physiopathology, Pulmonary Fibrosis diagnosis, Pulmonary Fibrosis drug therapy, Pulmonary Fibrosis physiopathology, Pyridones pharmacology, Respiratory Function Tests methods
- Abstract
Background: Pirfenidone has been shown to slow disease progression in patients with idiopathic pulmonary fibrosis (IPF). However, there are few treatment options for progressive fibrotic interstitial lung diseases (ILDs)) other than IPF. In view of the pathomechanistic and clinical similarities between IPF and other progressive fibrotic ILDs, we aimed to assess the efficacy and safety of pirfenidone in patients with four non-IPF progressive fibrotic ILDs., Methods: We did a multicentre, double-blind, randomised, placebo-controlled, parallel phase 2b trial (RELIEF) in 17 centres with expertise in ILD in Germany. Eligible participants were patients aged 18-80 years with progressive fibrotic ILD due to four diagnoses: collagen or vascular diseases (ie, connective tissue disease-associated ILDs), fibrotic non-specific interstitial pneumonia, chronic hypersensitivity pneumonitis, or asbestos-induced lung fibrosis. Other eligibility criteria included a forced vital capacity (FVC) of 40-90% predicted, a diffusing capacity of the lung for carbon monoxide of 10-90% predicted, and an annual decline of FVC of at least 5% predicted despite conventional therapy, based on at least three measurements within 6-24 months before enrolment. Patients who had received any previous antifibrotic therapy were excluded. We randomly assigned patients (1:1) to either oral pirfenidone (267 mg three times per day in week 1, 534 mg three times per day in week 2, and 801 mg three times per day thereafter) or matched placebo, added to their ongoing medication. Randomisation was done centrally using permuted block randomisation with varying block sizes stratified by the four diagnostic groups. Patients, investigators, statisticians, monitors, and the study coordinator were masked to treatment assignment until database closure. The placebo-controlled study period was 48 weeks (including up-titration). The primary endpoint was absolute change in percentage of predicted FVC (FVC % predicted) from baseline to week 48 in the intention-to-treat population, with imputation of missing data by the smallest sum of squared differences and attribution of deceased patients to the lowest rank in a rank ANCOVA model. Additionally, we did linear mixed-model repeated measures slope analyses of FVC % predicted longitudinal data over the course of the study as a prespecified sensitivity analysis and post-hoc sensitivity analyses of the primary endpoint in the intention-to-treat population using imputation methods of last observation carried forward [LOCF] and a regression-based multiple imputation procedure. Safety was assessed in all patients who received at least one dose of study medication. This trial is registered with EudraCT 2014-000861-32; DRKS00009822 and is no longer recruiting., Findings: Between April 5, 2016, and Oct 4, 2018, we randomly assigned 127 patients to treatment: 64 to pirfenidone, 63 to placebo. After 127 patients had been randomised, the study was prematurely terminated on the basis of an interim analysis for futility triggered by slow recruitment. After 48 weeks and in the overall population of 127 patients, rank ANCOVA with diagnostic group included as a factor showed a significantly lower decline in FVC % predicted in the pirfenidone group compared with placebo (p=0·043); the result was similar when the model was stratified by diagnostic group (p=0·042). A significant treatment effect was also observed when applying the LOCF and multiple imputation methods to analyses of the primary endpoint. The median difference (Hodges-Lehmann estimate) between pirfenidone and placebo groups for the primary endpoint was 1·69 FVC % predicted (95% CI -0·65 to 4·03). In the linear mixed-model repeated measures slope analysis of FVC % predicted, the estimated difference between treatment and placebo groups from baseline to week 48 was 3·53 FVC % predicted (95% CI 0·21 to 6·86) with imputation of deaths as prespecified, or 2·79 FVC % predicted (95% CI 0·03 to 5·54) without imputation. One death (non-respiratory) occurred in the pirfenidone group (2%) and five deaths (three of which were respiratory) occurred in the placebo group (8%). The most frequent serious adverse events in both groups were infections and infestations (five [8%] in the pirfenidone group, ten [16%] in the placebo group); general disorders including disease worsening (two [3%] in the pirfenidone group, seven [11%] in the placebo group); and cardiac disorders (one ([2%] in the pirfenidone group, 5 [8%] in the placebo group). Adverse events (grade 3-4) of nausea (two patients on pirfenidone, two on placebo), dyspnoea (one patient on pirfenidone, one on placebo), and diarrhoea (one patient on pirfenidone) were also observed., Interpretation: In view of the premature study termination, results should be interpreted with care. Nevertheless, our data suggest that in patients with fibrotic ILDs other than IPF who deteriorate despite conventional therapy, adding pirfenidone to existing treatment might attenuate disease progression as measured by decline in FVC., Funding: German Center for Lung Research, Roche Pharma., Competing Interests: Declaration of interests JB reports personal fees from Boehringer Ingelheim, Bristol Myers Squibb, Biogen, Galapagos, Promedior, Roche, and the German Center for Lung Research (DZL). SB reports personal fees and non-financial support from Roche, Bayer, Novartis, and Boehringer Ingelheim; personal fees from Merck Serono; and non-financial support from Teva, Gilead, Lucane Pharma, Actelion, CSL Behring, and Vertex. FB reports personal fees for consultancy, lecturing, and travel support from Boehringer Ingelheim and Roche. MC reports grants from DZL. JHF reports personal fees, grants, and non-financial support from Roche; and personal fees and non-financial support from Boehringer Ingelheim. AG reports grants from DZL, Boehringer Ingelheim, and Roche. MH reports grants from Actelion; honoraria for lectures from Actelion, Bayer Healthcare, Berlin Chemie, Boehringer Ingelheim, GlaxoSmithKline (GSK), Merck Sharp & Dohme (MSD), Novartis, OMT, Pfizer, and Roche; honoraria for advisory boards from Actelion, Bayer, Boehringer Ingelheim, GSK, MSD, and Roche; and honoraria for clinical trials from Actelion, Bayer, GSK, Pfizer, and United Therapeutics. NKn reports personal fees from Roche. DK reports personal fees from Roche; and personal fees and non-financial support from Boehringer Ingelheim. MK reports grants from DZL; and grants and personal fees from Boehringer Ingelheim and Roche. AP reports grants from DZL; grants, personal fees, and non-financial support from Boehringer Ingelheim and AstraZeneca; personal fees and non-financial support from Roche, Pliant, Chiesi Pharmaceuticals, and Nitto Denko; personal fees from Amgen; and non-financial support from Galapagos. KFR reports grants and personal fees from Boehringer Ingelheim and AstraZeneca; and personal fees from Novartis, Sanofi, Regeneron, Roche, and Chiesi Pharmaceuticals. TW reports grants from Roche; and personal fees from Boehringer Ingelheim, Roche, and Novartis. HWil reports personal fees for lectures or consultations from Actelion–Janssen, Bayer, Biotest, Boehringer Ingelheim, GSK, Pfizer, and Roche. HWir reports lecture fees from Roche. All other authors declare no competing interests., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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47. Perillyl alcohol suppresses monocrotaline-induced pulmonary arterial hypertension in rats via anti-remodeling, anti-oxidant, and anti-inflammatory effects.
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Beik A, Najafipour H, Joukar S, Rajabi S, Iranpour M, and Kordestani Z
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- Animals, Anti-Inflammatory Agents pharmacology, Antioxidants metabolism, Antioxidants pharmacology, Apoptosis drug effects, Blood Pressure drug effects, Cell Cycle drug effects, Disease Models, Animal, Heart Ventricles drug effects, Heart Ventricles pathology, Heart Ventricles physiopathology, Hemodynamics drug effects, Hypertrophy, Right Ventricular complications, Hypertrophy, Right Ventricular drug therapy, Hypertrophy, Right Ventricular physiopathology, Inflammation Mediators metabolism, Lung drug effects, Lung pathology, Lung physiopathology, Male, Monocrotaline, Monoterpenes pharmacology, Oxidative Stress drug effects, Pulmonary Arterial Hypertension chemically induced, Pulmonary Arterial Hypertension complications, Pulmonary Artery drug effects, Pulmonary Fibrosis complications, Pulmonary Fibrosis pathology, Pulmonary Fibrosis physiopathology, Rats, Wistar, Survival Analysis, Systole drug effects, Rats, Anti-Inflammatory Agents therapeutic use, Antioxidants therapeutic use, Monoterpenes therapeutic use, Pulmonary Arterial Hypertension drug therapy, Pulmonary Arterial Hypertension physiopathology, Vascular Remodeling drug effects
- Abstract
Background : Pulmonary arterial hypertension (PAH) is a disastrous disease that current treatments cannot prevent its progression. The present study investigated the effects of perillyl alcohol (PA), a natural monoterpene, on the experimental PAH in male Wistar rats. Methods : Rats divided into eight groups of control, Monocrotaline (MCT), MCT+vehicle, and MCT+PA with doses of 20, 30, 40, 50, and 60 mg/kg. PAH was induced by a single injection of monocrotaline (60 mg/kg) on day 0. The animals in the groups of MCT+vehicle and MCT+PA received the vehicle or PA from day 22 to 42 once a day. On day 43, under general anesthesia, right ventricular systolic pressure (RVSP), as an index of pulmonary artery systolic pressure, and the ratio of the right ventricle to the left ventricle plus septum weight, as the right ventricular hypertrophy index (RVHI), were measured. Also, some histological and biochemical indices were assessed in the lung tissue. Results : MCT significantly ( p < .001) enhanced the RVSP and RVHI compared to the control group (89.4 ± 8.2 vs 23 ± 3.3 mmHg & 0.63 ± 0.08 vs 0.26 ± 0.04 respectively). It also increased oxidative stress and inflammatory cytokines and reduced Bax/Bcl2 ratio. Treatment with PA significantly recovered RVSP and hypertrophy index and suppressed vascular cell proliferation, oxidant production, and inflammatory processes. Conclusion : PA exerted noticeable protective and curative effects against MCT-induced PAH and pulmonary vascular remodeling through inhibiting cellular proliferation, oxidative stress, and inflammation. Therefore, PA can be considered as a new therapeutic goal for the treatment of PAH.
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- 2021
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48. Clinical characteristics of non-idiopathic pulmonary fibrosis, progressive fibrosing interstitial lung diseases: A single-center retrospective study.
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Komatsu M, Yamamoto H, Kitaguchi Y, Kawakami S, Matsushita M, Uehara T, Kinjo T, Wada Y, Ichiyama T, Urushihata K, Ushiki A, Yasuo M, and Hanaoka M
- Subjects
- Adult, Aged, Aged, 80 and over, Humans, Immunosuppressive Agents therapeutic use, Japan epidemiology, Lung Diseases, Interstitial complications, Lung Diseases, Interstitial drug therapy, Lung Diseases, Interstitial mortality, Male, Middle Aged, Pulmonary Fibrosis drug therapy, Pulmonary Fibrosis etiology, Pulmonary Fibrosis mortality, Respiratory Function Tests, Retrospective Studies, Lung Diseases, Interstitial physiopathology, Pulmonary Fibrosis physiopathology
- Abstract
Competing Interests: The authors have no funding and conflicts of interests to disclose.
- Published
- 2021
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49. Antifibrotic drugs for pulmonary sarcoidosis: A treatment in search of an indication.
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Judson MA
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- Humans, Indoles therapeutic use, Pulmonary Fibrosis drug therapy, Pulmonary Fibrosis physiopathology, Sarcoidosis, Pulmonary physiopathology, Vital Capacity, Antifibrotic Agents therapeutic use, Pulmonary Fibrosis etiology, Pulmonary Fibrosis prevention & control, Sarcoidosis, Pulmonary complications, Sarcoidosis, Pulmonary drug therapy
- Published
- 2021
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50. Notch3 Deficiency Attenuates Pulmonary Fibrosis and Impedes Lung-Function Decline.
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Vera L, Garcia-Olloqui P, Petri E, Viñado AC, Valera PS, Blasco-Iturri Z, Calvo IA, Cenzano I, Ruppert C, Zulueta JJ, Prosper F, Saez B, and Pardo-Saganta A
- Subjects
- Actins metabolism, Animals, Bleomycin, Cell Differentiation, Cell Survival, Cells, Cultured, Disease Models, Animal, Disease Progression, Humans, Lung pathology, Lung physiopathology, Male, Mice, Inbred C57BL, Mice, Knockout, Myofibroblasts pathology, Phenotype, Pulmonary Fibrosis genetics, Pulmonary Fibrosis pathology, Pulmonary Fibrosis physiopathology, Receptor, Notch3 genetics, Mice, Collagen metabolism, Lung metabolism, Myofibroblasts metabolism, Pulmonary Fibrosis metabolism, Receptor, Notch3 deficiency
- Abstract
Fibroblast activation includes differentiation to myofibroblasts and is a key feature of organ fibrosis. The Notch pathway has been involved in myofibroblast differentiation in several tissues, including the lung. Here, we identify a subset of collagen-expressing cells in the lung that exhibit Notch3 activity at homeostasis. After injury, this activation increases, being found in αSMA-expressing myofibroblasts in the mouse and human fibrotic lung. Although previous studies suggest a contribution of Notch3 in stromal activation, in vivo evidence of the role of Notch3 in lung fibrosis remains unknown. In this study, we examine the effects of Notch3 deletion in pulmonary fibrosis and demonstrate that Notch3-deficient lungs are protected from lung injury with significantly reduced collagen deposition after bleomycin administration. The induction of profibrotic genes is reduced in bleomycin-treated Notch3- knockout lungs that consistently present fewer αSMA-positive myofibroblasts. As a result, the volume of healthy lung tissue is higher and lung function is improved in the absence of Notch3. Using in vitro cultures of lung primary fibroblasts, we confirmed that Notch3 participates in their survival and differentiation. Thus, Notch3 deficiency mitigates the development of lung fibrosis because of its role in mediating fibroblast activation. Our findings reveal a previously unidentified mechanism underlying lung fibrogenesis and provide a potential novel therapeutic approach to target pulmonary fibrosis.
- Published
- 2021
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